9/15/2016 Review Test Submission: quiz 2 – 1609_Protein Structure ...

Review Test Submission: quiz 2 – 1609_Protein Structure ...

Which of the following statement about the NMR magnet is NOT true? Selected Answer:

d.
Although cryoprobes increase the sensitivity of protein NMR, it also restricts the experimental temperature
to below 20 °C.
Answers:
a.
Tuning the probe is similar to tuning a radio to get a frequency match between the probe circuitry and the
RF transmitter.
b.
The superconducting coil which generates B0 is immersed in liquid helium.
c.
Most of the volume of an NMR spectrometer is taken up by liquid helium and liquid nitrogen.

d.
Although cryoprobes increase the sensitivity of protein NMR, it also restricts the experimental temperature
to below 20 °C.

Question 2

10 out of 10 points

Which nucleus is NOT commonly used for protein NMR in solution state? 

Selected Answer:

33
S

Which of the following is not true: 

Selected Answer:

In order to excite a transition from the alpha to the beta state in protons, radiofrequency (RF) pulses with a
frequency of 800 MHz are needed.

Answers:
All the protons within the magnets are precessing at 800 MHz.

In order to excite a transition from the alpha to the beta state in protons, radiofrequency (RF) pulses with a
frequency of 800 MHz are needed.

The computer controlling the magnetic has a 800 MHz CPU. 

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9/15/2016 Review Test Submission: quiz 2 – 1609_Protein Structure ...

Nuclear zeeman effect is larger on an 800 MHz spectrometer than a 600 MHz.

Question 4

10 out of 10 points

Which of the following statement is NOT true: 

Selected Answer:

The size of the bulk magnetization is proportional to gyromagnetic ratio.

Answers:
Gyromagnetic ratio the proportional factor for converting nuclear spin angular moment to magnetic moment

Gyromagnetic ratio can be positive and negative

The size of the bulk magnetization is proportional to gyromagnetic ratio. squared

Gyromagnetic ratio can be positive or negative. 

Question 5

10 out of 10 points

Which of the following is feasible way of detecting M0:

Selected Answer:

Because there is the large Bo in the same direction as Mo, we need to tip Mo away from Bo and detect the
precession of Mo around Bo. 

Answers:
Because Mo is very small, we can use an ultra sensitive compass. 

Because there is the large Bo in the same direction as Mo, we need to tip Mo away from Bo and detect the
precession of Mo around Bo. 

We can measure the heat absorbed by water in the magnet when we apply a resonant RF pulse. 

We can switch off Bo and instantly measure Mo before it relaxes way. 

Question 6

10 out of 10 points

In order to observe maximum NMR signal possible, we need to 

Selected Answer:
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9/15/2016 Review Test Submission: quiz 2 – 1609_Protein Structure ...

apply a resonant RF pulse that tips Mo exactly 90 degrees. 

Answers: apply strongest RF pulse possible at Larmor frequency.

apply a resonant RF pulse that tips Mo exactly 90 degrees. 

heat up the sample. 

weak RF pulse so selectively excite certain protons of interest. 

Question 7

10 out of 10 points

Which of the following statement about magnetic quantum number (m) is WRONG: 

Selected Answer:

Alpha state corresponds to m of -1/2 while beta state corresponds to 1/2. 

Answers:
m specifies the z-component of nuclear spin angular moment

can range from -I, -I+1, ... I. 

For deuterium, there are three possible m values. 

Alpha state corresponds to m of -1/2 while beta state corresponds to 1/2. 

Question 8

10 out of 10 points

Which of the following statement about rotating frame is FALSE? 

Selected Answer:

The rotating frame is not useful for understanding the effect of pulses in tipping Mo into xy plane. 

Answers:
The earth is a rotating frame as the earth rotates around its north-south axis every day, which can explains
the direction of air and water movement due to Coriolius force. 

A carousel is another rotating frame and the rotational motion of wooden horses disappears when you step
onto the carousel. 

In NMR, if we construct a rotating frame with Larmor frequency, in that frame, the spins are not precessing
any more and it is as if the Bo has been switched off. 

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9/15/2016 Review Test Submission: quiz 2 – 1609_Protein Structure ...

The rotating frame is not useful for understanding the effect of pulses in tipping Mo into xy plane. 

Question 9

10 out of 10 points

Which of the following statement is NOT true: 

Selected Answer:

Methyl groups usually have the largest chemical shift in the protein. 

Answers:
Amide protons have large chemical shift than alpha protons because nitrogens are more electron negative
than alpha carbons. 

Aromatic ring current can cause large changes in chemical shift. 

Methyl groups usually have the largest chemical shift in the protein. 

Chemical shifts can be measured in units of ppm or Hz but only ppm values are independent of Bo. 

Question 10

0 out of 10 points

Although RF pulses should have frequency of Larmor frequency (on the order hundreds of MHz), their B1
strength is also described in units of frequency, but usually on the order kHz, analogous Bo. Such frequency
describes how fast B1 can rotate the Mo. E.g., if an RF pulse has a B1 strength of 25 kHz, a 90 degree
pulse will be 

Selected Answer:

4 μs

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