INTODUCTION

Seed is one of the most important unit in crop production. It has a very significant values

in every individual farmers as well as the agriculturist even in early days. Seed are associated

with micro-organisms which prove harmful and can affect its capacity to germinate. This

microorganism can be a member of Important seed borne pathogen/ microorganism such as

various fungi, bacteria, viruses, nematodes etc.

The associated micro-organism may be pathogenic, weak parasite or saprophytes. It may

affect the seed internally or externally or as concomitant contamination as sclerotia, galls, fungal

bodies, bacterial ooze, infected plant parts, soil particles etc. mixed with the seed. Human

welfare as well as agriculture production can be negatively affected by this seed borne pathogen.

OBJECTIVES:

• To know how to culture the seed-borne pathogen by means of blotter test technique.

• To identify the cultured seed-borne pathogen on particular species.

METHODOLOGY

Students will collect Ten (10) seeds of any legumes species, which will then be put into

Blotter test. The seeds will be observed for several days minimum of one week, until it shows

signs of pathogenic activity to determine the seed incidence. The mold, as a result of pathogenic

activity, will be viewed in a microscope for a closer observation.

 For the preparation of blotter test technique, place a piece of tissue inside the base of

dishwashing paste container or any suitable container will do. Wet the tissue with water, put the

legume seeds on the wet tissue inside the paste container, then cover the container and observed

any changes on the seed.

RESULT AND DISCUSSION

The legume seeds (Ipip-ipil) were collected from any mother tree, developed seed-borne

organism, Several days after the seeds were put to a Blotter test, 6 out of 15 seeds showed signs

of pathogen infection. The mold appeared with white to green color over the surfaced of seeds.

Some seed doesn’t show any pathogen infection instead it undergo germination due to water

soaking

The specimen was observed in a microscope with 10x magnification. The image on the

microscope shows that the specimen has growth of hyphae and development mycelia indicating

that the pathogen is a type of fungi.

CONCLUSION

The most common seed-borne pathogen infecting the seed of Ipil-ipil is the bread mold.

Not all the seed can be infected by seed-borne pathogen even if they are putted in the same

container. The seed stored for long period of time must properly dried and contained no moist to

avoid development of pathogen.

 DISEASE INCIDENCE OF SEED

Exercise No. 3

Presented to
Prof. NELIA C. CALI
Department of Forestry
College of Forestry and Environmental Studies
Mindanao State University

Marawi City

In Partial Fulfillment
of the Requirements for the Course
FBS 155 (Forest Pathology) - XxYy
First Semester, AY 2019-2020

By:

Samporna, Saifodin S.
December 2019