BIOL2S32/Exam 3Fall2009%page 1 NAME:_Giroct Gain LD._Ol0F 7524) BIOL2533 Cell Biology Fall 2009 EXAM 3 | MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answors th | question. Cirle the answer on the exam and mark the appropriate box on the Scantrcn sea put your name and ID# on the Scantron answer sheet. R Scantron aro the final answers | accept. Each question is worth 2.5 points. Return both the exam and. | ‘scantron to me when you are finished. E 1 Which of the following is NOT a feature of the genetic code? a. degenerate d. triplet . universal, with rare exceptions @ 20 different codons for the 20 amino acids. . non-overlapping Vv 2. Which of the following is NOT associated with mRNA processing in eukaryotes? a. addition of a 5' methyl guanosine cap (@)removal of exons b. 3' polyadenylation e. both b and d ¢. removal of introns 3. ‘Anew antibiotic was identified that is able to bind and inactivate the tho factor associated with » ‘Sgime transcriptional processes; as a result this antibiotic would be expected to affect “a. initiation of transcription for all genes. ®. initiation of transcription for some genes. elongation by RNA polymerase at genes. (aeration of transcription at some genes. wet 29 diy oy sd @. termination of transcription at all genes. | 4. In eukaryctic cells the structural element is most functionally analogous to the Shine-Delgarno A (ribosomal binding site) sequence in prokaryotes is the: (Co) 7aholiy| gumnoane cap Onn ‘the Pribnow box 6. the TATA box in the core promoter the AAUAAA sequence in the mRNA «©, the poly A tail on mRNA + 5. Release factors of translation recognize which ofthe following codons? G a, AUG IGA: e. UUU GUA GGA Stop a. bacteria do not incorporate the DNA of other bacteria, b. attenuated rough bacteria can spontaneously revert back to virulent wild type bacteria. 8, DNA was conclusively the molecule of heredity (4. heat-killed bacteria could somehow “transform” live bacteria to a virulent form (smooth colonies). Ye. both ¢ and d are correct p 6. In 1928, Frederick Griffith's experiments with Streptococcus pneumonia suggested that Ee 7. Which expressions can be derived from of Chargaff's rules of bases within DNA? a. purines = pyrimidines d. bC+T cA= @all of the aboveBIOL2533/Exam 3/Fall2009/page 2 NAME: LD. a 8. DNA isolated from a newly identified bacterium has been ‘shown to have an adenine content of (C. 25%. Based upon this information, what is the %G+C within the DNA? = a. 0% J. 75% soe @. Answer cannot be derived from the information. '€)50% S. Following is part of the sequence of a segment of DNA. Using the known conventions of DNA { 6 Sequence representation, what is the correct sequence of the mRNA for which it codes? 5'CTTACGCGGTTAAC 3'-GAATGCGCCAATTG-5' GCATTC-3' d-6-CTTACGCGGTTAAC-3' b,) 5'CUUACGCGGUUAAC-3 e-S.GARTGCGCCAATTC-3 A _ 10. Inthe Sanger method of DNA sequencing, incorporation of the nucleotides stops because a. (C2 he dideoxynucleotide analogs have no 3'-OH group to continue DNA synthesis, “b* the dideoxynucleotide analogs have no 5OH group to continue DNA synthesis. . the dideoxynucleotide analogs have no 2-OH group to continue DNA synthesis, d. the mixture is heated to 92°C to stop DNA synthesis at specific time intervals. ®. the dideoxynucleotide analogs are in greater concentration than the actual nucleotides. 6 11. Which ofthe following DNA sequences could be a recognition ste fora restriction enzyme: a TCATCA d. AGGCGT G)GATATC crasnes e. TGCACA © GAATCC ace y 12. In eukaryotic transcription, TFIID (TBP) recognizes and.iods the site in the DNA. a. DPE (downstream promoter element) I TATA box b. BRE element ‘©. proximal promoter element . Inr element 13. The rate limiting step for transcription by RNA polymerase II is? binding of RNA polymerase II to the promoter © binding of TFIID (TBP) to the promoter. €. the phosphorylation of RNA polymerase by TFIIH 4. interaction of the sigma factor with the -10 box ¢. interaction of TFIIB and TFIIA with TFIID (TBP) RNA polymerase | d. RNA polymerase IV RNA polymerase I! e. RNA polymerase V ¢. RNA polymerase Ill {x mig Cukaryotic RNA polymerase synthesizes the larger rRNAs, like 28S, 18S and 5.8S RNAs? 2 (C15. The most abundant form of RNA is any cell is? a. hnRNAs 3. TRNAS: e. mRNAs b. tRNAs d. snRNAsBIOL2533/Exam 3/Fall2009/page 3 NAME: u 16. The greatest similarities among anticodons specifying the same amino acid occur ,and fh this are basis of the "Wobble hypothesis’. in the first two nucleotides of the triplet D. in the last two nucleotides of the triplet. c. in the first and third nucleotides of the triplet d. in the third nucleotide of the triplet €. in the middle nucleotide of the triplet few many histone molecules (individual protein molecules) are found in the nuclear histone core ticte? H2A a.1 8 © ats 4 10 “Dt a ons Mie 18. €aeh-transcription factor that stimulates transcription requires at least two domains that mediate "different aspects of their function. What are the minimum domains needed by a transcription factor to ‘stimalgie gene expression? the DNA-binding domain and the activation domain ‘the activation domain and the dimerization domain . the DNA-binding domain and a ligand-binding domain SX the RNA-binding domain and the activation domain ‘the RNA-binding domain and the repression domain A Wane, are four common DNA binding protein structures associated with transcription factors that we al looked at in class (Zn finger, helix-turn-helix, bZIP, and homeodomain). What recognizable structural Jement of the protein was common to all of these DNA-binding domains that inserted into the major groove of DNA, where it recognizes specific DNA base pair sequences by hydrogen-bonding? a. c-helix random coil b. parallel B-pleated sheet epee coil C. anti-parallel B-pleated sheet | 20. In what way does the bound IRP (iron regulatory protein) prevent translation? ~a, It causes degradation of the mRNA by exonucleases. It causes degradation of the mRNA by endonucleases. it interferes physically with the binding of a ribosome to the 5' end of the ferritin mRNA It interferes physically with the binding of a ribosome to the 3' end of the ferritin mRNA YR. It condenses the mRINA and causes it to bind to histones. Ave (C217 AtRINA has an anticodon with the sequence UAC. What would be the sequence of the complementary Codon, assuming the standard conventions of writing codons and anticodons. a. UAC 4. AUG 7 GUA e. ATG s UAC s b. (e pus 5 AUG B \ . In the trp operon, in the absence of tryptophan. a. the operator site is open to allow RNA polymerase binding b. the trp operon structural genes are transcribed C. the operator site is bound by the trp repressor to stop transcription aandb @.)a. bande[BIOL2538/Exam 1/Fall2009/page 4 NAME: u A 23. Approximately what percentage of the human genome sequence represents protein coding seq (a) 2% 4. 70% B. 50% e. virtually 100% ©. 60% G | 24..How are enhancers thought to stimulate transcription despite the large distance separating them from the core promoter? Enhancers can excise intervening DNA. & Enhancers and core promoters can be brought into ciose proximity because the intervening DNA can form a loop and held there through the interactions of DNA-bound proteins. . Enhancers can denature the DNA. d. Enhancers can search through DNA and bind directly to the associated core promoter. e. Enhancers bind RNA polymerase very tightly. A 25, Hoy-does binding of the Jac repressor to the lac operator block transcription initiation”? (a. lac repressor binding blocks RNA polymerase from interacting with DNA at the start site “b. lac repressor binding induces a DNase that cleaves the DNA at the transcription start site c. lac repressor binding causes a conformational change in RNA polymerase d. lac repressor binding induces a protease that degrades the sigma subunit of RNA polymerase @. none of the above C 26. In considering the Kozak consensus sequence for accurate AUG recognition in eukaryotic MRNA. {or transiation which base positions surrounding the AUG are most important. a. ~1 position and +4 position 4. -2 position and +5 position Px 2 position and +4 position €. -3 position and +5 position (c.)-3 position and +4 position ‘Adding or deleting nucleotides to the coding region of a gene in a DNA molecule affects the Teformation coded for by that gene. Which of the following additions or deletions would have the least G effect on the information coded for by that gene? a. adding one nucleotide to the gene. _d. deleting one nucleotide from the gene. b. adding two nucleotides to the gene. e. deleting two nucleotides from the gene. (Ce ddding three nucleotides to the gene <7 28. There are different enzymes responsible for linking amino acids to tRNAS and they are. Ce relorredbo ss £ ean a. ten, aminoacyHtRNA synthetases ten, amino acid transferases b. twenty, amino acid transferases twenty, aminoacyl-tRNA synthetases A c. sixty one, aminoacyl-tRNA synthetases 29. What role does glucocorticoid play in allowing a glucocorticoid receptor to enter the nucleus? '2) Binding of glucocorticoid causes the receptor to change shape, dissociates from HSP, and ‘enter the nucleus. . Binding of a glucocorticoid denatures the receptor allowing it through the nuclear pores. ‘c. The receptor is able to enter the nucleus in the absence of glucocorticoids. ~d. The glucocorticoid attaches a nuclear localization signal to the receptr. ~e. The receptor follows the glucocorticoid into the nucleus.BIOL2$33/Exam 3/Fall2009/page $ NAME: LD. 30. You isolate a new bacterium from soil and you are interested in characterizing the genome using restriction enzymes. The first enzyme chosen should be one that will cut the genome as infrequently as possible, allowing you to do more detailed fine mapping at a later date. Of the following choices, ie the best enzyme to choose for your task would be a, Haelll (GCC), -Sma\(Geecco) b. Mbol (GATC). lotl (GCGGCCGC). ¢. EcoRI (GAATTC). fc | 31. Except for the Met-tRNA used in translation initiation, all RNAs pass through the A, E, and P sites of the ribosome in a specific order. What is that order? a. Ato Eto P sites d. PtoEtoA sites b. PIO AtoE sites e. EtoP toAsites ©A0P 10 sites Match each of the following questions (32-35) with the appropriate statement about the mechanism of transcriptional repressor action a. competition b. quenching | c. interference | d. none of the above | e. all of the above | (| 82 Eunetions by blocking the assembly ofthe genera ranscription machinery atthe core promoter in eukaryotes, Re 6 || 83. This mechanism of repression involves the repressor protein directly binding the transcriptional | activator protein and preventing its association with DNA. (2 | | 34. This mechanism functions the repressor binding to the DNA in a manner that is mutually | exclusive to that of the transcriptional activator. | 85. This mechanism of repression involves the proteolytic degradation of the transcriptional activator D l to prevent its binding to DNA to stimulate transcription. | Match the indicated group(s) of organisms associated with the process or structure described | in questions 36 through 40. a. prokaryotes only c. prokaryotes and eukaryotes | | b. eukaryotes only d. none of the above (5 |26. The splicing of the mRNAs a common occurence. & | eo pr. AUG is the most common codon for translation. Yp [KR Uses RNA polymerase il to transcribe its RNAS. , C. [38 Contains 3 stop codons for translation termination, UAA, UAG, and UGA. C | Q |40. Has a -10 box and -35 box in the promoters of genes transcribed by RNA polymerase. fA |