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Advances in production technology

OF

By

P. VASUDEV NAIK
Assistant professor & Ph D scholar
22 September 2015 P. V. NAIK (Hort)
 Botanical name : Mentha spp.
Family : Labiatae(Lamiaceae)
 English name : Mint
 Indian name : Pudina, Putiha (Sanskrit)
 Pudina (Hindi & Kannada)
 Putina (Tamil)
 Podina (Telugu)

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• Genus Mentha there are several commercially
grown species, varying in their major chemical
content, aroma and end use. Their oils and
derived aroma compounds are traded world-
wide.

22 September 2015 P. V. NAIK 3


The four most commonly cultivated species are

Ø Japanese Mint/Menthol Mint (M.arvensis)


Ø Peppermint (M.piperita)
Ø Spearmint (M. spicata)
Ø Bergamot mint (M. citrata)

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Silent feature of mint crop

• All species are herbaceous plants, readily


sending out runners (rainy season) and
Stolons (winter), which develop new roots and
shoots at the nodes and form plants.

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• The entire aerial shoots together with foliage
is a source of essential oil rich in menthol,
Carvone, Linalool and Linanyl acetate having
use in pharmaceutical preparations and
flavour industry.

22 September 2015 P. V. NAIK 6


• For the past four decades, mints are
commercially cultivated in India. Of these, the
Japanese Mint, yielding menthol is grown
extensively in northern India.

• Other major producing countries are China. USA


and Brazil and to a smaller extent Thailand and
Vietnam.

22 September 2015 P. V. NAIK 7


Origin

• The cultivation of mint originated from Brazil


and China.
• Subsequently, China and India overtook Brazil
and more recently India has taken the leading
position in cultivation of this essential oil
yielding plant.

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Botanical Description
 Mint is a perennial ascending herb growing about 60-80 cm. in height
and under favourable conditions may attain a height upto 100 cm.
 It is propagated mainly by its stolons. Leaves are lanceolate-oblong,
sharply toothed; petiole is small about 5mm. in length.
 The leaf lamina varies from 5 to 15 cm. The leaf surfaces mainly lower
side is covered with dense hairy growth of glandular trichomes.
 Flowers are borne in axillary and terminal verticillaster , abundant in
number , purplish in colour.
 The flowers are small with corolla measuring 4-5mm., calyx 2-3mm.,
narrowly deltoid and acuminate.
 It does not produce seed and propagation is through vegetative means
only.
22 September 2015 P. V. NAIK 9
Economic Importance

 Japanese Mint (Mentha arvensis sub sp haplocalyx


var. piperascense) is an aromatic perennial herb,
grown as an annual in sub-tropical parts of north
India.
 The over-ground herb (foliage) on distillation yields
an essential oil, containing high (75 – 80%) menthol
content. The oil has a bitter cooling taste, harsh
odour and is the principal source of menthol.
 It is used in combating cold, used as an ingredient in
cough drops and related pharmaceuticals,
dentifrices, cosmetics, mouth washes, scenting of
tobacco products and flavouring of beverages.
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Cultivated mint species and their main constituents

Common name Botanical name Main constituents of mentha oil

Japanese mint Mentha arvensis Menthol (70-80 %)


Pepper mint M. Piperita Menthol (35-50 %)
Bergamot mint M.citrata Linalool and linalyl acetate (45 %)
Spear mint M. Spicata Carvone (60-95%)
Scotch spear mint M. cardiaca Carvone (53-68 %)
Garden mint M. viridis Carvone (very less)

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Area and Production

• At present, Japnese mint is cultivated in India on


about 103,000 ha. of land with estimated
production of 19,400 tonnes of mint oil which
accounts for about 55% of total menthol mint
production in the world.

Source: Essential Oils Association of India (2013),

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Estimated area and production of mint in India and abroad.

Species Area (ha.) Production Total world Major Producing


(tonnes of Oil) production Countries
(India)
(tonnes of Oil)
(India)

Japanese 103,000 19,400 34,860 India,China, Brazil,USA


mint
8,500 280 9,780 USA, France, former USSR,
Peppermint Brazil, India

Bergamot 4,200 65 492 USA , Brazil, Thailand


mint
23,000 380 4,050 USA,China, former USSR,
Spearmint India

Source: Essential Oils Association of India (2013),

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MARKET ANALYSIS AND STRATEGY
Demand and Supply Patterns

• The Mint oils obtained from Mentha arvensis, Mentha


piperita, Mentha citrata and Mentha spicata have put
India on the world map.

• Of these, mint oil and menthol are primarily exported


whereas others meet the home requirement of the
industry.

• Despite our quantity of peppermint and spearmint being at


par with World Standards, we have not made any headway
in export trade because of fierce competition by USA. The
latter is also the largest consumer of these oils.
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Export of mint oils, menthol and other derivatives from
India (2010-11 to 2013-14).

2010-2011 2011-2012 2012-2013 2013-2014


Item

Q V Q V Q V Q V

Mint oil
1292.0 32.2 757.0 22.5 1515.4 53.9 1421.5 50.9

Menthol
1798.4 215.3 1967.7 314.8 12809.0 567.4 8956.7 498.8

DMO
934.0 89.5 1238.6 34.3 2326.3 39.0 754.1 27.9

Total
Value - 337.0 - 371.6 - 660.3 - 579.6

Source: Monthly Statistics of Foreign Trade of India, Vol II Export)


22 September 2015 QNAIK
P. V. = Quantity (tonnes) ; V = Value (Rs. crores)
• Fluctuations in price are common. Currently it
varies between ₹.550-650/Kg for oil and
₹.900-1200/Kg for menthol (July-August and
October) but the price is also governed by
demand and price prevailing in importing
countries and speculations on volumes and
crop condition within India.

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Analysis and Future Strategy
 India has attained position of primary and dominant
source of mint oil and menthol in the world market,
replacing China to a great extent due to (lower) price
structure and comparable quality.

 At present, the growth in demand of oil and menthol


in world trade is slow and unless its demand rises
significantly in flavouring sector (where natural
products are preferred), there is little scope for any
major expansion in cultivation area.
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Analysis and Future Strategy...

 However, because of rising input cost and expensive labour


in South China, its cultivation there could presumably shrink
and consequently Indian mint may find increasing larger
market in next several years. There can be significant
increase in area of cultivation due to this.

 The country has evolved a number of new high yielding


varieties which has helped to maintain India in a lead
position. At present, India produces 4000 – 5000 tonnes of
DMO (dementholized oil) annually which is sold at a low
price.

 This has several minor constituents which could be


fractionated and marketed at higher price. Similarly, some
low value constituents of the DMO could be synthesized into
high value end products to help industry realize better value
of the primary product.
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Analysis and Future Strategy...

 At present, Cis – 3 hexanol is fractionated from DMO


and exported at a high price. Its production started in
1994-95 and this pushed price of DMO from ₹.50 to 90
in that year itself.
 There is scope for producing L-limonene, 3–octonol,
pure menthone and isomenthone. Similarly, L-menthol,
neo-menthol, isomenthone can be converted into high
value menthol and esters of menthol, like methyl
isovalerate, menthol lactate, methyl salicylate which
fetches better price in trade.
 All this can sustain a high level of cultivation and
support export trade. The market demand for pure L-
menthol, iso-menthone, L-limonene, neo-menthol and
pinene is high.
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Agro-climatic Requirements

• Mint can be cultivated both in tropical and sub-tropical areas.

• The mean temperature between 20-400 C during major part of


the growing period and rainfall between 100-110 cm. (light
showers at planting stage and ample sunshine at the time of harvesting)
is ideal for its cultivation.

• Well drained loam or sandy loam soils rich in organic matter


having pH between 6 and 8.2 are ideally suited for its
cultivation.

• It can also be cultivated on both red and black soil. In case of


acidic soil having pH less than 5.5, liming is recommended.
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Japanese

Bergamot Mint Pepper

Spear

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JAPANESE MINT (Mentha arvensis)
 Highly branched and grow upto 1 meter tall
 Oil content -0.5 -1%
 Broad ovate leaves
 Presence of glandular hairs on leaves, stem and calyx

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Sr. No. Variety Characteristics given in literature
1. MAS-1 § It is a dwarf variety 30-45 cm. in height and early maturing variety.
§ Less prone to insects due to short height.
§ Menthol content-70-80%.
§ Yield: About 200 q/ha. of herbage & 125-150 kg. of oil /ha.
2. Hybrid-77 § Early maturing variety.
§ It is 50-60 cm. in height.
§ Less prone to diseases viz. leaf spot & rust diseases.
§ Menthol content-80-85%.
§ Yield: About 250 q/ha. of herbage & 120-150 kg. of oil /ha.
§ It is especially well adapted to sandy loam soils and drier climate than that of the tarai
region.
3. Shivalik § The recovery of oil from the herb is 0.4 -0.5 %.
(selection from § Menthol content: 65-70%.
Chinese cultivar) § This variety is highly suitable for obtaining second cut through ratooning.
§ It is particularly grown in tarai region of U.P. & Uttaranchal.
§ The herbage yield is 300q/ha while the essential oil yield amounts to about 180 kg/ha.
§ Highly sensitive to the fungal diseases and pests prevailing in the tarai area.

4 Kushal § New variety developed through tissue culture.


§ Matures within 90-100 days.
§ The crop remains free from pests and diseases (particularly rust and leaf blight).
§ The variety is most suitable for transplanting after wheat in semi-arid –sub- tropical
condition of UP and Punjab.
§ It can withstand waterlogging for few days.
§ Yield: 300-330 q. /ha. having oil yield upto 175-200 kg./ha.
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5. EC-41911 § This is an erect type variety, which is less affected by rains.
(selection from
§ It produces 236.5 q/ha of herbage and 125.2 kg/ha of oil with
Russian
70% menthol.
germplasm)
§ Oil is preferred in flavouring food items.
6. Gomti § It is sturdy, light red in colour.
§ Menthol content-78-80%.
§ Yield is less compared to other varieties. It is less adopted by
farmers.
7. Himalaya § Resistant to rust, blight, mildew and leaf spot diseases.
§ Crop is good; size of leaves is larger than other varieties.
§ Menthol content-78-80%.
§ The yield of herbage is 400q/ha and essential oil is 200-250 kg/
ha.
8. Kosi § Matures early in 90 days.
§ Resistant to rust, blight, mildew and leaf spot diseases.
§ Essential oil content 75-80 % menthol.
§ Yield: 200-250 kg. of oil /ha.
9. Saksham § Developed through tissue culture from cv. Himalaya.
§ Yield: 225-250 kg. of oil/ha. having menthol content more than
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80%.
PEPPER MINT (Mentha piperita)

 Height of 45 -80 cm tall


 Oil content is 0.3-0.7 %

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3. CIM-Indus
It yields high menthoferan to meet
industrial demand in perfumery and pharma
sector (US Patent filed NF 449/2002).

4. CIM – Madhuras
• A sweet smelling genotype of peppermint.
• Used in food additives, flavors and
pharmaceutical formulations.
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SPEAR MINT (Mentha spicata)

Perennial herb
Height of 90 cm tall
 Oil content 0.6 %
 High carvone content

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Spearmint

1. CIMAP-MSS-1
2. CIMAP-MSS-5
3. CIMAP-MSS-98
4. Punjab spearmint-1
5. Ganga
6. Neerkalka

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1. MSS-1 (released CIMAP, Lucknow)

• This is a selection from the spearmint cultivars


introduced from USA.

• It gives a fresh herbage of 535 q and yield 253


kg of oil per hectare in 3 cuttings containing
60 per cent carvone.

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2. MSS-5
• It is a selection from MSS-1 (CIMAP,
Lucknow).

• This variety has an yield potential of 600 q


fresh herb and 349 kg/ha of oil from 3 cuttings.

• The carvone percentage in the oil is 70.

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3. Punjab spearmint-1
• This variety is a clonal selection made at
CIMAP, Lucknow.

• This variety has a erect growth habit with


quadrangular purple green hairy stem.

• It produces 209 q/ha of herb having 0.57 per


cent oil and can yield 119.29 t/ha oil with 68
per cent carvone.

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4. Ganga
A multipurpose culinary variety of Mentha
spicata var. Viridis leading to number of
products like disinfectant antimicrobials (NF
135/2000 US patent filed).

5. Neerkalka
Inter-specific hybrid between Mentha
arvensis and Mentha spicata with novel oil
quality, use in oral / dental care (US Patent PP
12,030).
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BERGAMOT MINT (Mentha citrata)

Branching, perennial herb


 60 cm tall
 oil content 0.4-0.5%
 oil emits lemon like smell

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1. Kiran
• This is a mutant selection developed by
mutation through gamma irradiation at
CIMAP, Lucknow.

• It has high quality oil content.

• This variety can yield 239 kg of oil per hectare


with 48 per cent linalool.

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Propagation

• Mint can be propagated vegetatively through stolons


and runners.

• by planting live juicy 8 to 10 cm.

• long stolons (underground stems) during early spring


season.

• The seed rate: 400-450 kg. of stolons per ha.

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Production of Stolons

• The plot should preferably be the best piece of land. It should be


given high level of FYM during land preparation.

• Around 200 sq.m. plot is required to produce stolons for 1


hectare.

• The nursery for the stolons is planted in August.

• Stolons are produced in autumn and are ready for use during the
months of January to March. To obtain the stolons , the soil is
opened manually or mechanically.
• These stolons can be used immediately or within a fortnight or so.
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Systems of Stolon production

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Sl. No. Materials Per acre Per hectare
1 Stolons (kg) 160 400
2 FYM (t) 16 40
Fertilizer (kg)
1 Spear mint N 48 120
P 20 50
K 16 40
2 Japanese mint N 64 160
P 20 50
K 16 40
3 Pepper mint N 50 125
P 20 50
K 16 40
4 Bergamot mint N 48 120
P 20 50
K 16 40
22 September 2015 P. V. NAIK 40
• In the plains, planting is done during winter months, whereas in
temperate climate, planting is done in autumn or spring from last week
of December to 1st week of March or from 1st week of January to 3rd
week of February.

• Late planting always gives poor yield.

• Mints require thoroughly ploughed, harrowed fine soil. All the stubbles
of weeds should be removed before the crop is planted.

• The stolons are cut into small pieces (7-10 cm) and planted in shallow
furrows of about 7-10 cm deep at a distance of 45-60 cm from row to
row manually or mechanically.

• Stolons are planted half way down on inner side of the ridges
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Commercial Raised bed methods of planting

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• Water requirement of mints is very high. Depending on soil and climatic
conditions the crop is irrigated 6-9 times before the first monsoon.

• The crop requires three irrigations after monsoon.

• Japanese mints require fifteen irrigations require getting maximum yield.

• Weed growth causes about 60 per cent reduction in herb and oil yield. Hence,
mints require weeding at regular intervals in the early stages of crop growth.

• Sinbar is effective as a post-emergence weedicide. Spray @ 1 kg per hectare.

• Organic mulch with combination of 0.5 kg oxyfluorfen herbicide per hectare


and weeding or Pendimethion herbicide at 1 kg per hectare and weeding has
been found to give excellent weed control throughout the crop growth

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Crop Rotation

 The rotation of mint crop with other food crops is


found to be a good way of controlling weeds.
Continuous cropping of any of the mints is not
advisable.

 The best rotation is Mint : Rice and Mint : Potatoes


and Mint : Vegetables : Peas etc. depending upon
cropping system followed in the region

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Scientific name of
Insects Nature of damage Control
insects
Hairy Diacrisia obliqua The caterpillar starts eating the Application of Thiodan
Caterpillar under-surface of the leaves. @1.7ml/ litre of water

Cutworms Agrotis flammatra Young plants are damaged at Soil treatment with
the collar region during spring Phorate 10g before planting.
season.

Red Pumpkin Aulocophora The pest feeds on the growing Spraying 5% dipterex
Beetle foevicollis leaves and buds.

Mint Leaf Syngamia The caterpillar folds the leaf in Two to three sprayings of
Roller abrupatalis the form of a roll and feed Thiodan @1.5ml/litre of
inside the leaf tissue during water at weekly intervals.
August-September. The edges of
the leaves are held together
22 September 2015 with silk-like filaments.
P. V. NAIK 46
Name of
Causal organism Symptoms Control
disease
Stolon Rot Macrophomina  During rainy season, stolon  Crop rotation. It is better to
phaseoli rot occurs on the follow 3-year-crop rotation
underground parts; the with rice, wheat and mint.
infected stolons show brown
 Treatment of the stolons
lesions which enlarge and
with 0.25% solution of
turn black, resulting in a soft
Captan or 0.1% Benlate, 0.3
decay.
% Agallol solution for 2 to 3
minutes before planting is a
preventive measure.

Fusarium Wilt Fusarium oxysporum  Affected leaves turn yellow,  Application of Benlate,
curled and finally dry. Bavistin and Topsin. @ 2g/l

Leaf blight Alternaria sp.  Cause loss of foliage during  Application of copper
summer season. fungicide @ 5%

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Leaf blight at early stage

Leaf blight at
severe stage

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Indices:
Generally the crop are harvested 100-120 days after planting
when the lower leaves start turning yellow. Further, harvesting should
be done in bright sunny weather.

 Harvesting consists of cutting the green herb by means of sickle 2-3 cm


above the ground.

A second harvest is obtained 80 days after the first harvest and the third
after about another 80 days.

 The first crop is ready by the end of June and the second in September or
October.

The average fresh yield from three cuttings is 20 to 25 t/ha


Essential 2015yield about 125-250 P.kg/ha.
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Mechanically harvesting of mint

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Shade drying

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Shade drying

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EXTRACTION AND UTILIZATION

o Harvested herb may be wilted in shade for a few hours


o Both fresh and dry herb used for distillation
o To get better result herbage should be shade dried for a
day before it is distilled
o Steam distillation usually preferred and duration of
distillation is 1.5-2 hours
o Impurities present in oil can removed by filtration
o Moisture present in oil can be removed by application
of anhydrous sodium sulphate
o GI or aluminium containers are suitable for long time
storage
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Extraction of Peppermint oil
Peppermint oil is extracted
from the whole plant
above ground just before
flowering.

The oil is extracted


commercially by steam
distillation
• Fresh or partly dried
plant herb
• Yield is 0.1 - 1.0 %.

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Water distillation

water

Water & steam Direct steam


distillation distillation

steam

water

Different
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P. V. NAIK apparatus 56
Steam distillation

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oil

water

oil

water

Different types of oil


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and water separators 58
P. V. NAIK
Supercritical fluid extraction (SFE)

It was performed by I. Gainar


et al. and was compared
with that of peppermint oil
isolated by hydro
distillation and found SFE is
better.

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Schematic diagram of SFE

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Microwave extraction
• The process is based on heat generated by ionic
conduction and/ or dipole rotation, and its efficiency
depends on the dielectric properties of the material.
• The extraction occurs when the water inside the plant
absorbs energy coming from the microwaves and
increases the pressure inside the material causing the
cell structure to break allowing the solvent to
penetrate into the matrix (Wang and Weller, 2006;
Chan et al., 2011; Eskilsson and Björklund, 2000;
Routray and Orsat, 2012).

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Industrial concrete production

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Plant for absolute production

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Ancient method for oil extraction

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Home extraction
• Easy method
• Suited for small sample extracts
• Time consuming
• Materials required
• Mason jar
• Mint leaves
• Grain alcohol or high-proof
• vodka
• Sieve, strainer, or cheesecloth
• Knife
• Funnel

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• Step 1: Mince fresh mint leaves with a knife. The finer the
leaves are chopped, the more surface area will be available
for oil extraction.
• Step 2: Place the chopped leaves into a glass mason jar. Fill
the jar with three parts of a high proof alcohol such as grain
alcohol or vodka for every one part minced leaves. The
alcohol will act as an agent to pull the oils out of the mint.
• Step 3: Cap the jar and shake well. Leave the container of
herbs to sit in a dark place for the next four to six weeks.
Shake the jar to stir up its continue every few days.
• Step 4: After the mint has soaked for four to six weeks,
separate the plant material from the liquid by pouring the
mixture into a clean bowl through a sieve or strainer. Wrap
the soaked leaves in a piece of fine cheese cloth and
squeeze out any captured extract.
• Step 5: Store the mint extract in small glass bottles or jars
in22 September
a cool 2015dark place. P. V. NAIK 66
Steps in extraction of mint oil

Step I Step II Step III

Step V
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Chemical constituents
• Various constituents of peppermint oil as per monographs of
International Pharmacopoeia are
 limonene (1.0-5.0%),
 cineole (3.5-14.0%),
 menthone (14.0-32.0%),
 menthofuran (1.0 -9.0%),
 isomenthone (1.5-10.0%),
 menthyl acetate (2.8-10.0%),
 isopulegol (max. 0.2%),
 menthol (30.0-55.0%),
 pulegone (max. 4.0%) and
 carvone (max. 1.0%).
• The ratio of cineole content to limonene content should be minimum
two.

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Standards for Evaluation
• International Pharmacopoeia monograph
• Relative density : 0.900 to 0.916.
• Refractive index : 1.457 to 1.467.
• Optical rotation : -10° to -30°.
• Specific gravity : 0.8997 to 0.9011
• Optical rotation : -370 11’ to 370 29’
• Acid value : maximum 1.4, determined on 5.0 g diluted in 50 ml of the
prescribed mixture of solvents.
• Fatty oils and resinified essential oils : Complies with the test for fatty oils and
resinified essential oils.
• Peppermint oil contains
• not less than 4.5 % w/w and not more than 10.0 per cent w/w of esters:
menthyl acetate, C12H22O2,
• not less than 44.0 per cent w/w of free alcohols: menthol, C10H20O, and
• not less than 15.0 per cent w/w and not more than 32.0 per cent w/w of
ketones: menthone, C10H18O.
• Chromatographic profiling of peppermint oil can be done with Gas
chromatography with flame ionization detector.
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Adultration
• Peppermint oil can be adulterated by addition of
much cheaper cornmint oil (Mentha arvensis).

• Peppermint oil blends well with: Although most


essential oils blend well with one another,
peppermint oil blends particularly well with
benzoic, eucalyptus, lavender, marjoram, lemon
and rosemary.

• Synthetic analogues of menthol can be prepared


in the labs
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Storage
Store in well-filled, tightly-closed, light-resistant
containers in a cool place.
Good quality PVC drum with screw caps: Short
term storage.
Galvanized iron drums or aluminium containers:
Long term storage.

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• Marketing:
– It is commonly sold in 1 oz. or 4 oz. bottles.

– US$ 13.50 for 10 ml

• Products

– Burners and vaporizers

– Blended oil or in the bath

– Mouthwash

– Cream or lotion

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Leaf proteome profiling of transgenic mint infected
with Alternaria alternata
• Ragini Sinha, Dipto Bhattacharyya, Aparupa Bose Majumdar, Riddhi Datta,
Saptarshi Hazra, Sharmila Chattopadhyay฀
Plant Biology Laboratory, Drug Development/Diagnostics & Biotechnology Division, CSIR-Indian Institute
of Chemical Biology, 4, Raja S.C.Mullick Road, Kolkata 700 032, India

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Ragini Sinha et. al., (2013)

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Hydrophobic derivatives of guar gum hydrolyzate
and gum Arabic as matrices for microencapsulation
of mint oil
Shatabhisa Sarkara, Sumit Guptab, Prasad S. Variyarb, Arun Sharmab, Rekha S. Singhala,∗
a. Food Engineering and Technology Department, Institute of Chemical Technology, Matunga,
Mumbai 400 019, India
b. Food Technology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India

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Paola Veronese et.al (2001)

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Paola Veronese et.al (2001)
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Paola Veronese et.al (2001)

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SOURCES OF INFORMATION

• Central Institute of Medicinal and Aromatic Plants,


Lucknow

• G. B. Pant University of Agriculture & Technology, Pant


Nagar – 263 145, District – Udham Singh Nagar,
Uttaranchal

• National Medicinal Plants Board, New

• Indian Horticulture Research Institute, Bangaluru.


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1. Dr. K Umesh, Professor of PMA, College of
Horticulture, Bangaluru.
2. Dr. Himabindu, Principle Scientiest, IIHR, Bangaluru
3. Dr V.K Rao, Principle Scientiest, IIHR, Bangaluru
4. Mr. Mahanthesh jogi P.hD (Hort) scholar.,
5. Mr. Udachappa pujare P.hD (Hort) scholar.,
6. Mr. saidulu, P.hD (Hort) scholar.,
7. www.google.com.
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