The Methods for Studying Microorganisms

Objective

In this lesson we will learn the following concepts:

 Microbes are managed and characterized by using the Five I's

(inoculation, incubation, isolation, inspection and identification).
 Various cultures and media are used in studying microorganisms.
 The parts of a microscope and how they are used for magnifying
cells and their parts.
 The different staining procedures for viewing
cells.

Lecture

Introduction
Growing up you were probably told to wash your hands so you would not
become infected by germs. You probably only did so to stay out of trouble
because no matter how well you focused on your hands, you never saw a
germ on them. Today you realize that a germ is a microorganism, one of
many that surround us. Microorganisms cannot be seen with the naked
eye, but you can see them with the aide of a microscope. In this lesson
you'll learn how to view microorganisms under a microscope.

The Five I's

The Five I's is a method used to locate, grow, observe and characterize
microorganisms. It is not necessary to perform all of these steps, or to
perform them exactly in this order. In some cases, one may proceed right
from the sample to inspection, and in others, only inoculation and
incubation on special media are required. The first step is the collect your
specimen. Common specimens are body fluids, foods, water or soil.

One you have your specimen collected you can perform the Five
I's:
1. Inoculation: The sample is placed into a container of sterile medium
that provides microbes with the appropriate nutrients to sustain

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 growth.

2. Incubation: An incubar can be used to adjust the proper growth

conditions of a sample.

3. Isolation: The end result of inoculation and incubation is isolation of

the microbe.

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 4. Inspection: The cultures are observed for obvious growth
characteristics that could be useful in analyzing the specimen
contents.

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 5. Identification: Determine the type of microbe, usually to the level of
species.

Md Ahsanul Haque
B.Sc in Microbiology
Gono Bishwabidyalay
Department Of Microbiology (Batch-37th)
E-mail: ahsanul192@gmail.com