NAME: ___________________________________ RATING: _________________

GROUP NO.: _____________________________ DATE: ___________________

ACTIVITY 7

PREPARING CULTURE MEDIA

A culture media is a special medium used in microbiological laboratories to grow different kinds of
microorganisms. A growth or a culture medium is composed of different nutrients that are essential
for microbial growth.

Since there are many types of microorganisms, each having unique properties and requiring specific
nutrients for growth, there are many types based on what nutrients they contain and what function
they play in the growth of microorganisms.

A culture may be solid or liquid. The solid culture media is composed of a brown jelly like substance
known as agar. Different nutrients and chemicals are added to it to allow the growth of different
microorganisms.

LEARNING OBJECTIVES:
At the end of the exercise, the students should be able to
1. Prepare different types of culture media
2. Explain the five basic techniques to manipulate, grow and examine, and characterize
microorganisms in the laboratory.
3. Discuss the classification of culture media.
4. Confer the proper maintenance and disposal of cultures.

MATERIALS: Microbiology book and e-book

PROCEDURE: Answer the following research questions.

RESEARCH QUESTIONS

1. Illustrate, define and give the purposes of the following

a. Nutrient broth
b. Nutrient Agar
c. Nutrient Agar Slant
d. Nutrient Agar Deep or Butt

1
 2. Explain the five basic techniques to manipulate, grow and examine, and characterize
microorganisms in the laboratory.
3. Discuss the classification of media based on the basis of
a. consistency
b. composition
c. purpose/functional use/ application
3. Confer the proper maintenance and disposal of cultures.

REFERENCES: