SBSc Agribiotechnology year III: GMO Test

1. What are the main differences which exist between plasmid vector
pUC18 and pBR322. How are these vectors different from expression vectors?
(5+10 marks)
2. What are the different steps which are involved in the construction
of a cDNA library? (10 marks)
3. How is the polyethylene glycol method of plant transformation different
from electroporation? (7.5 marks)
4. What are the essential features which a transformation vector need
to have for it to express a gene as a transgene. (10 marks)
5. How are selectable markers different from reporter genes? (7.5 marks)