Lab Service Manual of Clinical Labs1

Ministry of Health Issue No.
:2
Central Health Laboratories Issue Date: 1/1/2017
Title/ Number: Issued By: Dr. Dina Lithy
Lab service manual (LSM) Page No.: 1 of 39
Ministry of Health& Population

Central Public Health Laboratories
Lab service manual

Prepared by Reviewed by Approved by
Dr. SafenazGhareb Dr. Dina Ramadan Dr. Ahmed Safwat

Deputy of Quality manger Quality manger Head of CPHL
January 2017
Ministry of Health Issue No.:2
The information provided in this manual was current as of January 2017. Any changes or
new information superseding the information in this manual will be sent to you by mail or you
can found the update on the website of CPHL www.healthlabs.net
Purpose:
 To provide an overview for the laboratory service of clinical laboratories MOH to
customers. And define the method of contacts with lab representatives.
 To define instructions for proper sample collection and handling.
This manual will be available to those responsible for primary sample collection and for our clients.
Policy:
Prior to testing, all samples received by the Laboratory are assessed for acceptability. Criteria for
rejection are in place and are designed both to ensure accurate identification of samples and to
assess for optimal testing quality. In each instance where a sample is rejected, the patient care
provider is to be notified of the rejection. Once rejected, regardless of the reason, it is unacceptable
to process any rejected sample for testing until the cause of rejection removed. Questions from
physicians are to be directed to the laboratory specialist/residents
Table of content:
S. N. Contents Page
1 INTRODUCTION 4
2 LABORATORY REQUEST FORMS/ SPECIMEN CONTAINERS 4
3 IDENTIFYING THE PATIENT: 5
4 LABELLING THE SAMPLE 8
5 SPECIFIC REQUIREMENTS FOR SAMPLES COLLECTION 8
6 TRANSPORTATION & STORAGE OF SAMPLES 9
7 REPORTING OF TEST RESULT 9
8 COMPLAIN 9
9 CUSTOMER SURVEY 9
10 Appendix 10
11 lists of available laboratory examination 11

1. introduction
1-Scope
Central health laboratory includes services primarily reported to evaluate specimens obtained from
patients to provide information to the treating physician. This information, coupled with
information obtained from requester
If after a test is ordered and performed, additional tests are ordered, these tests would be considered
separate of the ordered test and need another request.
2-Purpose:
To produce high quality lab service
3- Contact details
Address:19ElShakhRehanst –AbdeenCairo -
Workinghours:from9am –to 4 pm from Saturday – Thursday
Tel. (202)27948544-27950096 extension (401) Fax.
(202)27948544
4- Clinicalconsultant:
Dr: dalalMoneir
Tel: 01227432434
Email:d.moneir@hotmail.com
5- Quality manager: Dr. Dina Ramadan Lithy

Tel:01222661449
E-mail:dinalithy@yahoo.com
2. Laboratory request forms/ specimen containers:

1. Lab request form
Clinical data are useful for better service, so please fill all data required in our request form.
2. Procedures for requesting additional tests
If additional tests are required, another request form is a must.
We don`t accept phone request.
3. Repeating examination:
Repeating examination due to analytical failure or further examinations of the same primary
sample are done whenever needed.
3. Identifying the patient:

1. Clinical information:
Clinical information can help in identification of patient’s e.g.
 Clinical history and any correlated lab tests should mentioned or accompanied with the
request.
 Drug history
 Provisional diagnosis
2. Information provided to patients in relation to their own preparation

Special preparations are needed in some tests.
3. Instruction for patient collecting samples
Will be provided in waiting room
4. Patient consent
For disclosure clinical information and family history to relevant healthcare professional is needed
in case of use of referral lab .
‫‪Ministry of Health‬‬ ‫‪Issue No.:2‬‬
‫‪Central Health Laboratories‬‬ ‫‪Issue Date: 1/1/2017‬‬
‫‪Title/ Number:‬‬ ‫‪Issued By: Dr. Dina Lithy‬‬
‫)‪Lab service manual (LSM‬‬ ‫‪Page No.: 6 of 39‬‬
‫‪Detailed information on sample collection requirements‬‬
‫‪Patient identification‬‬
‫‪Tests requested‬‬
‫)‪Minimum volume 2 ml whole blood in plain tube (yellow cover‬‬
‫‪Time and date of the sample collection‬‬
‫‪Appropriate clinical data, when indicated‬‬
‫‪ . 4‬خطوات إستقبال العمالء إلجراء التحاليل الطبية‬

‫‪:4.1‬دخول العميل ‪-:‬‬
‫يدخل العميل من الباب الخلفى للمعامل (بوابة الفلكى) وياخذ رقم مسلسل من موظف االمن ثم يتوجه‬
‫الى الخزينه او مكتب المطالبات حسب نوع المعاملة‪.‬‬
‫‪:4.2‬انواع المعامالت ‪-:‬‬

‫‪ ‬مكتب المطالبات‪:‬‬
‫يتوجه العميل فى حالة تحويله من هيئة التأمين الصحى او العالج على نفقة الدولة الى مكتب‬
‫المطالبات بعد استيفاء االوراق المطلوبة (اصل جواب التامين الصحى او القرار‪ -‬صورة جواب التامين‬
‫الصحى او القرار‪ -‬صورة بطاقة او كارنيه التامين الصحى) ليتم اعتماد االوراق من الموظف المختص‬
‫ويقوم بتوجيه العميل الى غرفة سحب العينات‪.‬‬
‫‪ ‬الـخـزينــة‪:‬‬
‫يتوجه العميل فى حالة اجراء التحاليل الطبية على نفقته الخاصه الى خزينة االدارة المركزية والتى‬
‫من خاللها يقوم الموظف المختص بتقييم التحاليل الطبية وتسعيرها طبقا‬
‫( لقرار ‪ ) 205‬واعطاء العميل ايصال بالقيمة المدفوعة ومن ثم يقوم بتوجيه العميل الى غرفة سحب العينات‪.‬‬
‫‪:4.3‬غرف سحب العينات‪-:‬‬

‫‪ ‬المخدرات والعقاقير الطبية‪:‬‬
‫تستقبل غرفة سحب العينات رقم (‪ )1‬العمالء راغبى تحاليل المخدرات والعقاقير الطبية ويقوم موظف االستقبال‬
‫باستيفاء شروط اخذ العينة وتسجيل كافة البيانات والتحاليل المطلوبة على الكمبيوتر وطباعة باركود التحاليل وايصال‬
‫استالم النتائج ومن ثم يقوم بتوجيه العميل الى المكان المخصص لسحب العينة‪.‬‬
‫‪( ‬الهرمونات – ابحاث الدم – الكيمياء – الفيروسات) االطفـال ‪:‬‬
‫تستقبل غرفة سحب العينات رقم (‪ )7‬االطفال حتى سن ‪ 10‬سنوات والتى فيها يقوم موظف االستقبال باستيفاء‬
‫شروط اخذ العينة وتسجيل كافة البيانات والتحاليل المطلوبة على الكمبيوتر وطباعة باركود التحاليل وايصال استالم‬
‫‪Ministry of Health‬‬ ‫‪Issue No.:2‬‬
‫‪Central Health Laboratories‬‬ ‫‪Issue Date: 1/1/2017‬‬
‫‪Title/ Number:‬‬ ‫‪Issued By: Dr. Dina Lithy‬‬
‫)‪Lab service manual (LSM‬‬ ‫‪Page No.: 7 of 39‬‬
‫النتائج ومن ثم يقوم بتوجيه العميل الى المكان المخصص لسحب العينة حيث يقوم فنى المعمل بسحب العينة والتاكد من‬
‫تطبيق شروط اخذ العينة‪.‬‬
‫‪( ‬الهرمونات – ابحاث الدم – الكيمياء – الفيروسات) الكبـار‪:‬‬
‫تستقبل غرفة سحب العينات رقم (‪ )13‬العمالء التى تتجاوز اعمارهم الـ‪ 10‬سنوات والتى فيها يقوم موظف‬
‫االستقبال باستيفاء شروط اخذ العينة وتسجيل كافة البيانات والتحاليل المطلوبة على الكمبيوتر وطباعة باركود التحاليل‬
‫وايصال استالم النتائج ومن ثم يقوم بتوجيه العميل الى المكان المخصص لسحب العينة حيث يقوم فنى المعمل بسحب‬
‫العينة والتاكد من تطبيق شروط اخذ العينة‪.‬‬
‫‪:4.4‬غرفة استالم العينات الخارجية‪-:‬‬

‫تستقبل غرفة استالم العينات رقم (‪ )8‬العمالء والمندوبين القادمين بعينات خارجية من المستشفيات ويقوم موظف‬
‫االستقبال باستيفاء شروط استالم العينة وتسجيل كافة البيانات والتحاليل المطلوبة على الكمبيوتر وطباعة باركود‬
‫التحاليل وايصال استالم النتائج‬
‫‪2‬البيانات الواجب توافرها لمريض المعمل ‪:‬‬
‫‪ o‬االسم ثالثي ‪ :‬حتى ال يحدث خطأ أثناء أخذ العينات من حيث األسماء‪.‬‬
‫‪ o‬العنـــوان ‪ :‬المحافظة واالدارة التابع لها خاصة فى االطفال حديثى الوالدة حتى يمكننا متابعة الطفل‬
‫‪ o‬رقم التليفون ‪ :‬حتى يتثنى لطبيب المعمل االتصال بالمريض إلبالغه اذا استدعى إعادة التحليل فى بعض‬
‫الحاالت‪.‬‬
‫‪ o‬الســـن ‪ :‬ألنه يوجد لكل سن نسب طبيعية في الدم‪.‬‬
‫‪ o‬التحاليل المطلوبة‬
‫ال يقبل اى طلبات فحص بالتليفون وال يتم ابالغ النتائج تليفونيا تسلم النتائج للمريض موقعة من الطبيب المختص‬
Ministry of Health& population Issue No.:2
Central Public Health Laboratories Issue Date: 25/10/2015
4. Labeling the samples:

1. Identification of the primary sample
Sample received at the sample receiving unit, registered on Lab. information system.
2. Recording the identity of the person collecting the primary sample
The name of primary sample collector is registered on Lab. information system.
3. Labeling of primary sample:
After registering primary sample onLab.Informationsystem, unique identified number is
applied to primary sample container side. (Barcode)
5. Specific requirements for samples collection:

1. Primary sample collection instructions:
Blood Collection: Blood specimens will be collected in a manner that ensures proper
testing and reduces the possibility of cross contamination and erroneous results.
CPHL Specimens collected using the Vacationer system; Color coded collection tubes are
used according to the specimen type required namely serum, plasma or
wholebloodaccording to the specification of each test.
2.General rules for sample acceptance & rejection

 Unlabeled sample.
 Broken or leaking tube/container.
 Insufficient /incorrect details concerning to labeling or to request form.
 Sample collected in wrong tube.
 Request form and sample label do not match
 Inadequate sample type.
 Inadequate sample volume.
 Poor handling during transportation.
 Sample collected in non-sterile tube (for certain samples only mentioned before).
 Absence of swabs (for Influenza or corona samples).
 Absence of VTM (for Influenza or corona samples).
 Prolonged transport time &poor handling during transport
 Improper timing for sampling ( provided a list for time for specific samples)
NOTE:
 Rejected samples may be accepted after the reason of rejection is reconciled.
 Receipt of Unsuitable Specimen is prohibited
3. Receipt of Mislabeled or Unlabeled samples:

 Before receipt of sample, inform the collection person immediately that the
specimen is unlabeled or mislabeled.
 If the sample is not or cannot be identified, cancel the sample with the comment
(mislabeled sample) and sent back to the requester with notification.
4. Time needed between collection and received by the laboratory (transport

requirements, refrigeration, etc.………..) All samples should be transported in tightly
closed container in icebox
5.Disposal of waste material used in specimen collection

Needles sharp objects: safety box
Gloves and alcohol pads: red box
Urine and stool collection cups: red box
Any other waste: black box
6.Transportation and Storage of samples

The samples which received from places out of our sample collection unite must follow the
regulation of collection, package and transportation of it according to the type of sample
and the test needed to be examined as the following , Sample examined retained whenever
needed according to different lab tests and variable sample types and will be stored in
suitable condition
Regulation of collection and transportation of samples:
7. Reporting of test results:

1. Reporting of results:
Reporting of results done through written reports signed by specialist and approved by lab
director and delivered in reporting room.
The report can be sent either by fax or delivered to authorized person upon your request.
2. Telephone reports
Not available
3. Emergency reports:
The client or requester will be informed immediately in case of emergency report by phone
8. Complain
In case of any complain of our service contact the responsible person through complain
form .
9. Customer survey
The customer survey found in attachment
10. Appendices:
 Appendix 1: Request form
 Appendix 2: Request form for TB
 Appendix 3: Policy of central health laboratory
 Appendix4: Customer survey
 Appendix 5 complain
 Appendix 6: result form
 Appendix 7: delay report
11-lists of available laboratory examination
transpor
tation
Patient
Min. and
NO. Test Specimen type TAT Rejection Causes Preparatio Reference range Technique Clinical significance
quantity storage
n
conditio
ns
Up to the
mark of Unlabeled HbA : 96.7-98
Hb purple or ,mislabeled, Careful HbF : < 1 Diagnosis of
1 Whole EDTA blood 1W Ice box Chromatography
Electrophoresis lavender wrong tube, wrong history Hb A2 : 2.2- 3.5 thalassemia
top tube amount or clotted
Up to the
mark of
unlabeled ,mislabeled Between
purple or Diagnosis of G6PD
2 G6PD screening Whole EDTA blood 1W Ice box ,wrong tube, wrong hemolytic 4.6-13.5 u/g Hb Photometric
lavender deficiency
amount or clotted attacks
top tube
Careful
FII : 70120%
history
Up to the FVII :70-120%
including
mark of unlabeled ,mislabeled FX : 70-120% Diagnosis of
Coagulation Plasma from citrated drugs&
3 blue top 1W Ice box ,wrong tube, wrong 4FVIII: 70-1450% Bioactivity method coagulation factor
factor assay blood plasma or
tube amount or clotted FIX:70-4120% defects
blood
F5XI:70-120%
transfusio
FXII : 70-120%
n
Up to the unlabeled Careful Diagnosis coagulation
Plasma from citrated Ag. : 50-160 IU
VWF mark of 1W Ice box ,mislabeled, wrong history ELISA disorders ( VWF
4 blood Act. : 60-138 IU
blue top tube, wrong amount including deficiency)
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
tube or clotted drugs&
plasma or
blood
transfusio
n
Careful
history
Up to the unlabeled including Diagnosis coagulation
PT:75-100%
Plasma from citrated mark of ,mislabeled, wrong drugs& disorders, monitor
5 PT & PTT Same day Ice box PTT : 35-45 Bioactivity method
blood blue top tube, wrong amount plasma or heparin and oral
seconds
tube or clotted blood anticoagulants.
transfusio
n
Careful IgM : < 11
unlabeled ,mislabeled
Anticardiolipin history MPLU/ML Diagnosis of
6 Serum 0.5 ml 2W Ice box ,wrong tube, wrong ELISA
antibodies including Ig G : < 23 GpL hypercoagulable state
amount
drugs U/ML
Up to the Careful
Protein C and Plasma from citrated mark of history PC : 72-160 IU Diagnosis of
7 1W Ice box ,wrong tube, wrong ELISA
Protein S. blood blue top including PS : 60-150 IU hypercoagulable state.
amount or clotted
tube drugs
Up to the unlabeled Careful
Lupus Plasma from citrated mark of ,mislabeled, wrong history PT assay of patient Diagnosis of
8 Same day Ice box 31-44 seconds
anticoagulant blood blue top tube, wrong amount including &normal plasma hypercoagulable state
tube or clotted drugs
Careful
Up to the history
Platelet Plasma from citrated mark of including ADP : 70-110% Up to the mark of Diagnosis of Platelet
9 Same day Ice box ,wrong tube, wrong
aggregation blood blue top drugs& Rist. : 70- 110% blue top tube function
amount or clotted
tube plasma or
blood
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
transfusio
n
Up to the
unlabeled
mark of No special Manual staining & Diagnosis of types of
Reticulocyte ,mislabeled, wrong Adult: 0.5-2.5%
10 Whole EDTA blood purple or Same day Ice box preparatio microscopic anemia& monitor of
count tube, wrong amount Infant : 2-5%
lavender n examination iron therapy
or clotted
top tube
Up to the
mark of unlabeled ,mislabeled No special
Positive or Diagnosis of sickle cell
11 Sickling test Whole EDTA blood purple or Same day Ice box ,wrong tube, wrong preparatio Sickling test
Negative anemia
lavender amount or clotted n
top tube
Up to the
mark of unlabeled ,mislabeled No special
Identification of blood
12 ABO-Rh grouping Whole EDTA blood purple or Same day Ice box ,wrong tube, wrong preparatio Slide agglutination
group
lavender amount or clotted n
top tube
Up to the
unlabeled
mark of No special
,mislabeled, wrong Positive or
13 Malaria rapid test Whole EDTA blood purple or Same day Ice box preparatio Malaria rapid test Diagnosis of Malaria
tube, wrong amount Negative
lavender n
or clotted
top tube
Up to the unlabeled
No special st
Whole citrated mark of ,mislabeled, wrong 1 hour : up to 10 Up to the mark of
14 ESR Same day Ice box preparatio nd
blood black top tube, wrong amount 2 hour : up to 20 black top tube
n
tube or clotted
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
antigen antibody
No
unlabeled reaction by
special Diagnosis of
15 ASOT Serum 0.5 ml Same day Ice box ,mislabeled, wrong 0-200 quantitative & semi
tube, wrong amount
preparati rheumatic fever
quantitative latex
on
agglutination
antigen antibody
No
unlabeled Negative up to 6 reaction by CRP is used mainly
special
16 CRP Serum 0.5 ml Same day Ice box ,mislabeled, wrong mg/L quantitative & semi as a marker of
preparati
tube, wrong amount quantitative latex inflammation
on
agglutination
antigen antibody
No
unlabeled reaction by
17 RF Serum 0.5 ml Same day Ice box ,mislabeled, wrong 0-30 IU/mL quantitative & semi
preparati Rheumatoid disease.
tube, wrong amount quantitative latex
on
agglutination
antigen antibody
No
unlabeled Positive or reaction by
special
18 Monospot Serum 0.5 ml Same day Ice box ,mislabeled, wrong Negative quantitative & semi Diagnosis of IMN
preparati
tube, wrong amount ( qualitative) quantitative latex
on
agglutination
antigen antibody
No
unlabeled Positive or reaction by
19 Rose Waller Serum 0.5 ml Same day Ice box ,mislabeled, wrong Negative quantitative & semi
preparati Rheumatoid disease.
tube, wrong amount ( qualitative quantitative latex
on
agglutination
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
Normal Range :
4.2 - 6.2
Diabetic : > 7
Up to the
No Estimate the
Glycated mark of unlabeled
Whole EDTA special Good Control glycaemic control
20 hemoglobin ( purple or 48 h Ice box ,mislabeled, wrong Chromatography
blood preparati 5.5 - 6.8 over the past 3
HB A1c) lavender tube, wrong amount
on Fair Control month
top tube
:6.8 - 7.6
Poor Control :
Above 7.6 %
Iron measurements
Males : 65
are used in the
– 170 ug/dl
diagnosis and
Females : 5 0 –
treatment of iron
No 170 ug/dl
unlabeled deficiency anemia,
special Child : 50 Bichromatic rate
21 Iron Serum 0.5 ml 1W Ice box ,mislabeled, wrong hemochromatosis
preparati – 120 ug/dl technique
tube, wrong amount Iron determinations
on Infant : 40–
are Performed for
100 ug/dl
the diagnosis and
New born : 100
monitoring of
- 250 ug/dl
microcytic anemia.
Males : 250
– 450 ug/dl Useful in differential
No Females : 250 diagnosis of anemia,
Total iron Unlabeled
special – 450 ug/dl increased in
22 binding capacity Serum 0.5 ml 1W Ice box ,mislabeled, wrong Colorimetric
preparati Child : 250 thalassemia &
(TIBC) tube, wrong amount
on – 400 ug/dl decreased in iron
Infant : 100 – deficiency anemia.
400 ug/dl
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
No
Unlabeled Useful in differential
special
23 Ferritin Serum 0.5 ml 2W Ice box ,mislabeled, wrong 21-385 ng/ml ELISA diagnosis of
preparati
tube, wrong amount microcytic anemia.
on
Up to the Used to diagnose
Unlabeled No
mark of Cell Counter & anemia, infections
Complete blood Whole blood on ,mislabeled,wrong special According to
24 purple or Next day Ice box microscopic , leukemia and other
count (CBC) EDTA tube, wrong amount preparati sex & age
lavender examination hematological
or clotted on
top tube disorders.
Negative
No +1 weak
Unlabeled
special positive Immunofluorescenc Diagnosis of
25 ANA Serum 0.5 ml 1W Ice box ,mislabeled, wrong
preparati +2mod. Positive e immune disorders
tube, wrong amount
on +3Strong
positive
Negative
No +1 weak
Unlabeled
special positive Immunofluorescenc Diagnosis of
26 ASMA Serum 0.5 ml 1W Ice box ,mislabeled ,wrong
preparati +2mod. Positive e immune disorders
tube, wrong amount
on +3Strong
positive
Negative
Unlabeled No +1 weak
,mislabeled special positive Immunofluorescenc Diagnosis of
27 AMA Serum 0.5 ml 1W Ice box
,wrong tube, wrong preparati +2mod. Positive e immune disorders
amount on +3Strong
positive
Unlabeled No Negative Immunofluorescenc Diagnosis of
28 ASM Serum 0.5 ml 1W Ice box
,mislabeled, special +1 weak e immune disorders
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
wrong tube, wrong preparati positive
amount on +2mod. Positive
+3Strong
positive
Negative < 0-
200 IU/ml
Unlabeled No Weak positive
,mislabeled special 201-300 Diagnosis of
29 Ds DNA Serum 0.5 ml 1W Ice box ELISA
,wrong tube, wrong preparati Mod. Positive immune disorders
amount on 301-800
Strong positive
> 800
Unlabeled No
,mislabeled, special Negative <20u Immunofluorescenc Diagnosis of
30 ANCA Serum 0.5 ml 1W Ice box
wrong tube, wrong preparati Positive > 25u e immune disorders
amount on
Unlabeled No
,mislabeled, special Negative <20u Diagnosis of
31 LKM Serum 0.5 ml 1W Ice box ELISA
wrong tube, wrong preparati Positive > 25u immune disorders
amount on
Negative < 20u
Weak positive
Unlabeled No
20-39u
,mislabeled special Immunofluorescenc Diagnosis of
32 ACCP Serum 0.5 ml 1W Ice box Mod. Positive
,wrong tube, wrong preparati e immune disorders
40-59u
amount on
Strong positive
> 60u
3-5 2-8°c - Hemoptysis - Morning
33 Direct smear Pulmonary(sputum
3-5 ml days - Less than the sample Negative Ziel Nelsen stain
Preliminary
microscopy and BAL)and minimum amount before diagnosis of T.B.
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
(DSM). Extra pulmonary breakfast
8 2-8°c - Hemoptysis - Morning - Detection of
Culture by Pulmonary(sputum)a weeks - Less than the sample Ptroff Mycobacteria.
34 conventional 5-10 ml minimum amount before Negative
nd extra pulmonary Method -For Antibiotic
method. breakfast sensitivity testing.
Treatment of T.B. -
Drug Sensitivity Contaminated or very Patients
35 T.B. positive culture 4 weeks 2-8°c Proportional
testing (DST) weak culture Diagnosis and survey -
of MDR cases
Hemoptysis - Rapid Diagnosis of
Culture by MGIT
36 Sample suspension 3-5 ml 42 days 2-8°c Less than the - -------- Negative ---------------- Mycobacterium
960.
minimum amount tuberculosis Complex
Treatment of T.B.
Sensitinty by Contaminated or very Patients
37 -TB Positive culture 2-14 days 2-8°c - - -
MGIT 960. weak culture Diagnosis and survey -
of MDR cases
Culture,
Wash
Identification & Immedi Culture on Mac,
Long time after urethra
37 AMST Urine 7-10 ml ately ------ .Blood agar Diagnosis of UTI
days 3 Sampling with
(antimicrobial BR. MH.agar for AST
water
susceptibility test)
Culture,
Identification & Can be Came with out
Not need Diagnosis of
AMST on Cary transport media and SS agar
38 Stool 5-10 gm 3-5 days preparatio -------- food poisoning and
(antimicrobial Blair unknown time of BR. MH.agar for AST
n typhoid fever
susceptibility Media sampling
)test
Culture, 10 ml for 7-21 days Incubat Blood with out - Clean Culture on plates Diagnosis of PUO
Blood -
39 Identification & adult e after culture bottle site of Blood. , choc. Mac.
AMST 1.5-2ml in put the injection Confirmed tests for
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
(antimicrobial culture blood - Feverish gm +ve or gm-ve
susceptibility test) bottle for patient organisms
child
Culture, Culture on plates

In aims Diagnosis of
Identification & Throat , wound, ear, Blood. , choc. Mac.
media Dry swab without - No use pharyngitis
40 AMST and 3 days Confirmed tests for
transport media anti septic Wound infection and
(antimicrobial eye swab gm +ve or gm-ve
Eye infection
susceptibility test organisms
Put on - Not on TIM
Taken by
TIM -Sample taken more
Identification & physician
valid for than 1 hour Culture identification Diagnosis of
41 serotyping of CSF 1-2 ml 3 days not
6ms. At AST and can be PCR meningitis
Meningitis Technicia
room
n
temp
-Sample
as
Leather
can be any sample paper
(Leather, paper, Depend on …not
Detection of B. Culture on Blood
42 sputum,bl.) specimen 4days need Diagnosis of Anthrax
anthracis. agar.
type storage
- Human
sample
immedi
ately
Neisseria CSF for culture or ml 2-1 days3 Keep Unknown time of PCR For Diagnosis of
43 meningitides,Ha for PCR on sampling meningitis
emophilusinflue chocola
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
nza and te agar
or
crayovi
als
vails in
freezer
Cholera. Serotyping by 5days TCBS ------ Sterile or has Slide agg. Detect type of
44 antisera plates pathogenic cholera
organism
Sterility test for Any sample 4-5days No Not enough amount -------- Sterile or has Culture on different Identification of
any samples transpo - Open back age pathogenic plate media sterile and non-
Adequate
45 rt - Sample differ organism B.R. sterile specimen
amount
media from what written
in request
Fasting :
( 74 - 100 )
mg/dL Diagnosis
unlabeled Random : diabetesmellitus,
,mislabeled, Fasting ( up to 200 ) Colorimetric Enzyme neonatalhypoglycemia
46 Glucose Serum 2.0 ml 1D Ice box wrong tube, wrong from mg/dL Method .
amount or 6 – 8 H. Post Prandial Pancreatic islet cell
Hemolyzed less than carcinoma.
140mg/dL
Fasting 74 –
unlabeled Diagnosis diabetes
100 mg/dL
,mislabeled, Fasting mellitus ,neonatal
Glucose tolerance 30 min up to Colorimetric Enzyme
47 Serum, 2.0 ml 1D Ice box wrong tube, wrong from hypoglycemia .
test 190 mg/dL Method
amount or 10 – 16 H. Pancreatic islet cell
1 hr up to 180
Hemolyzed carcinoma.
mg/dL
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
1,30 hr up to 170
mg/dL
In the diagnosis and
treatment of
numerous renal and
unlabeled Men 3.4 - 7.0
metabolic disorders
,mislabeled, No special mg/dl
Uric Acid Colorimetric Enzyme including renal failure
48 Serum 2.0 ml 1D Ice box wrong tube, wrong preparatio
Method gout,leukemia,psoriasi
amount or n Women 2.4 - 5.7
s,starvation or other
Hemolyzed mg/dl .
wasting conditions
and patient receiving
cytotoxic drugs.
Urea measurements
unlabeled
are used in the
,mislabeled, No special
Urea Serum, EDTA& 10 - 50 mg/dl diagnosis and
49 2.0 ml 1D Ice box wrong tube, wrong preparatio Enzymatic Kinetic
heparin plasma treatment of certain
amount or n
renal and metabolic
Hemolyzed
diseases .
unlabeled Assessment of kidney
Males 0.8 –
Serum, EDTA& ,mislabeled, No special function diagnosis and
1.1mg/dl
50 Creatinine heparin 2.0 ml 1D Ice box wrong tube, wrong preparatio Colorimetric treatment of kidney
Females 0.5 – 0.9
plasma amount or n
mg/dl
Hemolyzed disease .
Increases in serum
PTH or vitamin D are
unlabeled
associated with hyper
Calcium (Total) Serum, EDTA& calcemia, which may
51 2.0 ml 1D Ice box wrong tube, wrong preparatio 8.1 - 10.4 mg/dl Colorimetric
heparin plasma lead to multiple
amount or n
myeloma and other
Hemolyzed
neoplastic diseases .
Hypocalcemia may be
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
observed in
hypoparathyroidism ,
nephrosis and
pancreatitis .
unlabeled
Diagnosis coagulation
Estimated 4.2 – 5.3 mg/dl disorders, monitor
52 Calcium (Ionized) ---- 1D Ice box wrong tube, wrong preparatio ---------------------------
heparin and oral
amount or n
anticoagulants.
Hemolyzed
unlabeled Diagnosis and
,mislabeled, No special treatment parathyroid
Serum, EDTA& Endpoint and signal
53 Phosphorus 2.0 ml 1D Ice box wrong tube, wrong preparatio 2.7 - 4.5 mg/dl and kidney diseases,
heparin plasma point calibration
amount or n and vitamin D
Hemolyzed Imbalance .
unlabeled
,mislabeled,
Diagnosis and
wrong tube, wrong No special
Serum, heparin 135 - 152 Ion Selective treatment OF
54 Sodium 2.0 ml 1D Ice box amount or preparatio
plasma mmol/l electrode dehydration and over
Hemolyzed n
hydration.
Diagnosis and
unlabeled
treatment of diabetic
Serum, heparin 3.5 - 5.2 m mol Ion Selective coma, renal
55 Potassium 2.0 ml 1D Ice box wrong tube, wrong preparatio
plasma /l electrode failure,severe fluid and
amount or n
electrolyte loss, effect
Hemolyzed
of certain drugs.
unlabeled Effect of Lithium on
No special
Serum, heparin ,mislabeled, Less than 0.2 Ion Selective other laboratory test
56 Lithium 3.0 ml 1D Ice box preparatio
plasma wrong tube, wrong mmol /l electrode values .
n
amount or Increased TSH in 30%
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
Hemolyzed of patient (
clinicallyeuthyroid ) .
Increased
parathormone with
resultant increased
serum calcium and
decreases phosphorus.
Decreased serum
testosterone.
May affect THR,
growth hormone ADH
Serum lithium values
may be increased by :
Decreased glomerular
filtration rate ( GFR)
(e.g. , aging )
Sodium deprivation
and dehydration.
Serum lithium values
may be decreased .
By increased GFR (e.g.
pregnancy
,hemodialysis )
In burn patient .
Diagnosis and
unlabeled Normal :
treatment of liver,
,mislabeled, No special Total Up To 1.0
Bilirubin (Total - Serum, EDTA& Colorimetric hemolytichematologic
57 2.0 ml 1D Ice box wrong tube, wrong preparatio mg/dl.
direct) heparin plasma al , and metabolic
amount or n Direct up to 0.2
disorders, hepatitis
Hemolyzed mg/dl
and gall bladder block.
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
unlabeled
Diagnosis and
,mislabeled, Men up to
Alanine No special treatment of certain
Serum, EDTA& wrong tube, wrong 40u/l IFCC
58 Aminotransferase 2.0 ml 1D Ice box preparatio liver diseases (eg viral
heparin plasma amount or Women up to
(ALT) n hepatitis and cirrhosis
Hemolyzed 31 u/l
) and heart diseases.
unlabeled Men up to
Myocardial infarction,
Aspartate ,mislabeled, No special L37u/l
Serum, EDTA& hepatic disease,
59 Aminotrasferase 2.0 ml 1D Ice box wrong tube, wrong preparatio Women up to 31 IFCC
heparin plasma muscular dystrophy
(AST) amount or n u/
and organ damage .
Hemolyzed
Diagnosis treatment
and investigation of
unlabeled hepatobiliary diseases
Adult :30 - 120
Alkaline ,mislabeled, No special and in bone disease
Serum, EDTA& u/l Colorimetric
60 phosphatase 2.0 ml 1D Ice box wrong tube, wrong preparatio associated with
heparin plasma Children : 47 -406
amount or n increased osteoblastic
u/l
Hemolyzed activity .diagnosis of
parathyroid and
intestinal disease.
Diagnosis and
unlabeled Normal : treatment of liver,
,mislabeled, No special Total Up To 1.0 hemolytichaematologi
Bilirubin (Total - Serum, EDTA& Colorimetric
61 2.0 ml 1D Ice box wrong tube, wrong preparatio mg/dl. cal , and metabolic
direct) heparin plasma
amount or n Direct up to 0.2 disorders, hepatitis
Hemolyzed mg/dl and gall bladder block.
Alanine unlabeled No special Men up to Diagnosis and

Serum, EDTA& IFCC
62 Aminotransferase 2.0 ml 1D Ice box ,mislabeled, preparatio 40u/l treatment of certain
heparin plasma
(ALT) wrong tube, wrong n Women up to liver diseases (eg viral
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
amount or 31 u/l hepatitis and cirrhosis
Hemolyzed ) and heart diseases.
Unlabeled Men up to
Myocardial infarction,
Aspartate ,mislabeled, No special L37u/l
Serum, EDTA& hepatic disease,
63 Aminotrasferase 2.0 ml 1D Ice box wrong tube, wrong preparatio Women up to 31 IFCC
heparin plasma muscular dystrophy
(AST) amount or n u/
and organ damage .
Hemolyzed
Diagnosis treatment
and investigation of
Unlabeled hepatobiliary diseases
Adult :30 - 120
Alkaline ,mislabeled, No special and in bone disease
Serum, EDTA& u/l Colorimetric
64 phosphatase 2.0 ml 1D Ice box wrong tube, wrong preparatio associated with
heparin plasma Children : 47 -406
amount or n increased osteoblastic
u/l
Hemolyzed activity .diagnosis of
parathyroid and
intestinal disease.
Diagnosis and
treatment of
Unlabeled
numerous diseases
Serum, EDTA& Colorimetric involving primarily the
65 Albumin 2.0 ml 1D Ice box wrong tube, wrong preparatio Adult 3.8 - 4.4
heparin plasma liver or kidneys.
amount or n g/dl Neonates :
Hemolyzed 3.8 - 4.2 g/dl
Diagnosis and
Unlabeled
treatment of diseases
Protein Serum, EDTA& Colorimetric involving the
66 2.0 ml 1D Ice box wrong tube, wrong preparatio 6.4 – 8.3 g/dl
totalSerum heparin plasma liver,kidney and bone
amount or n
marrow .
Hemolyzed
Metabolic or
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
nutritional
Diagnosis and
Unlabeled treatment of liver
Male : 11 - 50
Gamma Glutamyl ,mislabeled, No special diseases such as
Serum, EDTA& U/L Colorimetric
67 Transferase 2.0 ml 1D Ice box wrong tube, wrong preparatio alcoholic cirrhosis and
heparin plasma Females: 7 – 32
( GGT ) amount or n primary and secondary
U/L
Hemolyzed liver tumors.
Indicator for
theperformance of
No special
Estimated Estimated liver and kidneys in
68 A/G ratio ----- 1D ----- ------------- preparatio 1 – 2
the body, and can aid
n
in an early detection
of certain diseases
Unlabeled
,mislabeled, Diagnosis and
No special
Cholesterol wrong tube, wrong 115 - 200 Colorimetric Enzyme treatment of lipid
69 Serum 2.0 ml 1D Ice box preparatio
amount or mg/dl Method lipoprotein metabolic
n
Hemolyzed disorders
Diagnosis and
treatment of diseases
Unlabeled
involving lipid
,mislabeled, Fasting
Serum, EDTA& Colorimetric metabolism and
70 Triglycerides 0.5 ml 1D Ice box wrong tube, wrong from 12 - 36 - 150 mg/dl
heparin plasma various endocrine
amount or 14 H.
disorders e.g. diabetes
Hemolyzed
mellitus , nephritis and
liver obstruction .
Unlabeled No special . An inverse
Serum, EDTA&
71 HDL cholesterol 0.5ml 1D Ice box ,mislabeled, preparatio 35 – 70 mg/dl Colorimetric relationship between
heparin plasma
wrong tube, wrong n HDL-cholesterol (HDL-
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
amount or C)levels in serum and
Hemolyzed incidence/prevalence
of coronary heart
disease
- An relationship
between HDL-
cholesterol (HDL-
LDL cholesterol Estimated
72 ---- 1D ------------- ------------ 65 – 170 mg/dl ------------ C)levels in serum and
incidence/prevalence
of coronary heart
disease
0-4 day 290 -775 Diagnosis and
U/l treatment of liver
Unlabeledmislabeled, 4-10 days 545- diseases such as acute
Lactate No special
Whole blood on wrong tube, wrong 2000U/ viral hepatitis,cirrhosis
73 dehydrogenase 0.5 ml 1D Ice box preparatio Enzymatic
EDTA amount or 10-24 m 180-430 and metastatic
(LDH) n
Hemolyzed u/l carcinoma of the liver ,
>12 y 100 – 210 cardiac of the lung or
u/l kidneys.
Diagnosis and
Unlabeledmislabeled, treatment of diseases
No special
wrong tube, wrong of the pancreas such
74 Lipase Serum 0.5 ml 1D Ice box preparatio 5.6 – 51.3 U/L COLORIMETRIC
amount or as pancreatitis and
n
Hemolyzed obstruction of the
pancreatic duct .
3 Months : 67 – Determining inherited
Unlabeledmislabeled, 124 mg/dl or acquired
No special
wrong tube, wrong 6 Month : 74 – Immunoturbidimetric deficiencies .
75 C3 Serum 0.5 ml 1W Ice box preparatio
amount or 138 mg/dl assay Rise in a variety of
n
Hemolyzed 9 Month : 78 – inflammatory and
144 mg/dl necrotic disorders as
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
12Mont78h : – part of the acute-
150 mg/dl phase plasma protein
2 – 10 years 80 – response.
150 mg/dl
12 – 18 years 85 –
160 mg/dl
3 Months : 9 –
30.5 mg/dl
6 Month : 10 – 35 Determining inherited
mg/dl or acquired
Unlabeledmislabeled, 9 Month : 11.5- deficiencies. Rise in
No special
wrong tube, wrong 39 mg/dl Immunoturbidimetric inflammatory and
76 C4 Serum 0.5 ml 1W Ice box preparatio
amount or 12Month : 12 – assay necrotic disorders as
n
Hemolyzed 40 mg/dl part of acute-phase
2 – 10 years plasma protein
12.5 – 42.5 mg/dl response.
12 – 18 years 14 –
43
In AIDS serum B2M an
accurate predicator of
the progression of the
disease in HIV infected
group including
Unlabeledmislabeled,
No special haemophilias . Level
wrong tube, wrong
B2microglobin Serum 0.5 ml 1W Ice box preparatio 0.9 - 3.0 mg/l Turbidimetric above 5 mg/l indicate
amount or
n rapid progression and
Hemolyzed
poor prognosis in
Various myelomas,and
effectiveness of
treatment levels
77
below 4 mg/l indicate
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
better prognosis than
levels above 20 mg/l.
New born 1.5
Unlabeledmislabeled, IU/ml
No special
IgE Total Serum, EDTA& wrong tube, wrong Up to 1 Y. 15.0 Immunoturbidimetric Aid in the diagnosis of
78 0.5 ml 1W Ice box preparatio
heparin plasma amount or IU/ml assay allergic diseases.
n
Hemolyzed Children 1-5 Y.
60.0 IU/ml
Adults : 90 - 450
Measurement of IgA
mg/dl
aids in the diagnosis of
Unlabeledmislabeled, Children (8-10 Y.)
No special abnormal protein
Serum, EDTA& wrong tube, wrong : Immunoturbidimetric
79 IgA 0.5 ml 1W Ice box preparatio metabolism and
heparin plasma amount or 96 - 206 mg/dl assay
n body´s lack of ability
Hemolyzed Neonates (2 - 8
to resist infectious
D.) :
agents .
0.0 - 1.3 mg/dl
Adults : 800 -
1800 mg/dl Measurement of
Children (8-10 Y.) immunoglobulin G
Unlabeledmislabeled,
No special : aids in the diagnosis of
Serum, EDTA& wrong tube, wrong Immunoturbidimetric
89 IgG 0.5 ml 1W Ice box preparatio 855 - 1255 mg/dl abnormal protein
heparin plasma amount or assay
n Neonates (2 - 8 metabolism and the
Hemolyzed
D.) : body´s lack of ability
863 - 1465 to resist infections .
mg/dl
Adults : Men 60- Increased in : liver
250mg/dl disease
Unlabeled ,mislabeled No special
Serum, EDTA& Women 70 – 280 Immunoturbidimetric Chronic infection
90 IgM 0.5 ml 1W Ice box ,wrong tube, wrong preparatio
heparin plasma mg/dl assay Depressed in protein-
amount n
Children 8 10 Y. losing syndromes
74 – 142 mg/dl Non –IgM myeloma
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
Infancy ,early
Neonates (2 - 8 ,childhood
D.) :
5 – 37 mg/dl
Its primary use is in

diagnosis of preclinical
states of Wilson
disease .
Ceruloplasmin is also
an acute-phase recant
protein whose
concentration may rise
to as high as three
Unlabeledmislabeled, times the upper
No special
Ceruloplasmin Serum, EDTA& wrong tube, wrong Immunoturbidimetric reference limit during
91 0.5 ml 1W Ice box preparatio 20 - 60 mg/dl
heparin plasma amount or assay bacterial infections
n
Hemolyzed .concentration are also
raised in a variety of
neoplastic and
disease,primary biliary
cirrhosis,system lupus
erythematosis and
rheumatoid arthritis.
Stool analysis No special Parasite-Fasciola –

Random sample 1D. Ice box
preparatio Bilharzias –
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
n Ancylostoma –
Mass (carcinoma,,
adenoma )
Inflammation –
vascular disorders –
infection (T.B,
amebiasis ,hookworm
Alerts on
whipworm,
the
strongyloidiasis
patient's
ascariasis ) – epistaxis
Occult blood in eating Negative
92 Random sample 1D. Ice box Cartridge ,oropharynx .
stool dairy
Factitious,
products
coagulopathies.
only for 3
Asymptomatic
days
ulcerated lesion of G I
T.
Carcinoma of the
colon and large
adenoma .
Bact diagnosis
–Pyelonephritis-
No special
prostatitis
93 Urine analysis Random sample 1D Ice box preparatio
Candida –
n
Some cancer tumor
marked like
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
benceJonce protein in
multiple myeloma .
Detection of Glucose
in Diabetic patient
detection of ptn in
many disease like
G.nephritis or renal
failure.
Bile & urobilinogen
and ketone substance
.
kidney disease
Albumin/creatinin diabetic nephropathy.
94 Random sample 1D Ice box
e Ratio
kidney disease
Protein in 24 hr ;diabetic nephropathy
95 24 H. urine 1D Ice box
urine
Creatinine 0.5ml serum + R. Assessment of kidney

96 1D Ice box
clearance Sample of urine function
* Small volume,
Ice box /
Adulterated,
8℃ Emit
Cannabinoid 1-4 working Not tightly sealed
97 Urine 20 ml 7days No needs Cut off 50 ug/ml &chromatography Addict
(Marijuana) days
Then on
-(2-8)℃
Ice box / Small volume, Emit
1-4 working
98 Opiates Urine 20 ml 8℃ Adulterated, No needs Cut off 300 ug/ml &chromatography Addict
days
7days Not tightly sealed
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
Then on
-(2-8)℃
Ice box / Small volume,
8℃ Adulterated, Emit
1-4 working
99 Barbiturates Urine 20 ml 7days Not tightly sealed No needs Cut off 300 ug/ml &chromatography Addict
days
Then on
-(2-8)℃
1-4 working
100 Benzodiazepines Urine 20 ml 7days Not tightly sealed No needs Cut off 200 ug/ml &chromatography Addict
days
Then on
-(2-8)℃
1-4 working
101 Amphetamines Urine 20 ml 7days Not tightly sealed No needs Cut off 500 ug/ml &chromatography Addict
days
Then on
-(2-8)℃
8℃7day Adulterated, Emit
1-4 working
102 Tramadol Urine 20 ml s Not tightly sealed No needs Cut off 200 ug/ml &chromatography Addict
days
Then on
-(2-8)℃
8℃ Adulterated,
1-4 working &chromatography
103 Cocaine Urine 20 ml 7days Not tightly sealed No needs Positive /Negative Addict
days
Then on
-(2-8)℃
Phencyclidine 1-4 working 8℃7day Adulterated, &chromatography
104 Urine 20 ml No needs Positive /Negative Addict
(PCP) days s Not tightly sealed
Then on
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
-(2-8)℃
Small volume,
Ice box /
Hemolyzed ,
8℃ Emit & Redox
1-4 working
105 Ethyl Alcohol serum 0.5-2 ml 2days No needs Positive /Negative Reaction May be Addict
days
Then on
-(2-8)℃
2 hrs
before the
Ice box / Small volume, next dose
8℃ Hemolyzed , Or at the
Emit Dose adjusted
106 Valproic acid serum 0.5-2 ml 4 hrs 2days mid time 50-100µg/ml
according to result
Then on between
-(2-8)℃ 2
consecuti
ve doses.
2 hrs
before the
Ice box / Small volume, next dose
8℃ Hemolyzed , Or at the
Dose adjusted
107 Carbamazepine serum 0.5-2 ml 4 hrs 2days mid time 4-12 µg/ml Emit
according to result
Then on between
-(2-8)℃ 2
consecuti
ve doses.
2 hrs
before the 10-20µg/ml
8℃ Hemolyzed ,
next dose adults 6- Dose adjusted
108 Phenytoin serum 0.5-2 ml 4 hrs 2days Emit
Or at the 14 µg/ml child according to result
Then on
mid time
-(2-8)℃
between
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
2
consecuti
ve doses.
1 cc whole
109 TSH serum blood uU/mL 9.1 - 0.8
sample
*Closed
1 cc whole
contain 2.69 - 1.05
110 FT3 serum blood For adults :
ers ng/mL
sample 24 hrs Detecting thyroid
*room No
1 cc whole For Electrochemoluminen malfunction
tempera General rejection specific 28.3 - 10.3
FT4 serum blood childern ces on cobas e411 Follow up of thyroid
111 ture(avo criteria preparatio Pmol/L
sample less than 2 and cobas e601 dysfunction patients
id n required
1 cc whole years : 3 under treatment
freezing
112 T3 serum blood hours Pmol/L 9.8 - 2.4
)
sample
1 cc whole
113 T4 serum blood ug/dL 16.0 - 6.5
sample
- (ELIZA)
-qualitative detection
-No or of antibodies to
incompletedata Human
sheet, -mis-matched ImmunoDefficiency
data in sheet &on Viruses (HIV) type 1
Cold Detection of
114 HIV 1/2 Ab Serum 0.5 ml 5 wds tube,-bad Non More than 1 (group M-O), type 2in
chain antibodies to the Virus
transportation human serum.
conditions, -not -chemiluminescent
enough quantity, - microparticle
broken tubes Immunoassay (CMIA)
for the simultaneous
qualitative detection
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
of HIV p24 antigen
and antibodies to
human immune
deficiency virus type
1 and/or type 2 (HIV-
11/HIV-2) in human
serum
Enzyme Linked
Immunoassay (ELIZA)
for in-vitro qualitative
detection of hepatitis
B surface antigen
(HBsAg) in human
serum.
Cold Detection of
115 HBsAg Serum 0.5 ml 3wds As above Non More than 1 And
chemiluminescent
Immunoassay (CMIA)
for the qualitative
detection of hepatitis
B surface antigen in
human serum
chemiluminescent
Immunoassay (CMIA)
Cold for the qualitative Detection of
116 HCV Ab Serum 0.5 ml 3wds As above Non More than 1
chain detection of antibody antibodies to the Virus
to hepatitis C virus in
human serum
Cold Above the cut off Detection of
117 (CMV) IgM/IgG. Serum 0.5 ml 3wds As above Non CMIA
chain ( Qualitative) antibodies to the Virus
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
118 anti-HAV. Serum 0.5 ml 3wds As above Non CMIA
119 anti-HBc . Serum 0.5 ml 3wds As above Non CMIA
120 anti-HBs. Serum 0.5 ml 3wds As above Non CMIA
Rubella virus Cold Above the cut off Detection of
121 Serum 0.5 ml 3wds As above Non CMIA and ELISA
IgG/IgM . chain ( Qualitative) antibodies to the Virus
122 Toxo IgG. Serum 0.5 ml 3wds As above Non CMIA
Measles virus Cold Above the cut off Detection of
123 Serum 0.5 ml 3wds As above Non ELISA
IgM. chain ( Qualitative) antibodies to the Virus
124 (RVF) IgG/IgM Serum 0.5 ml 3wds As above Non ELISA
125 Mumps IgM Serum 0.5 ml 3wds As above Non ELISA
Cold Indirect Detection of
126 Schistosomiasis Serum 0.5 ml 3wds As above Non More than 1/80
chain haemaglutination antibodies to the Virus
Cold Detection of
127 Syphilis Serum 0.5 ml 3wds As above Non More than 1/64 ELISA
As above& using
Cold Detection Limit= Detection of activity of
128 Rt- PCR HCV Serum 0.5 ml 5wds nonsterile tubes, no Non Rt-PCR
chain 21 IU/ml the virus
VTM or No swab
Cold Detection Limit= 9 Detection of activity of
129 Rt- PCR HBV Serum 0.5 ml 5wds As above Non Rt-PCR
chain IU/ml the virus
Cold Detection Limit= Detection of activity of
130 Rt- PCR HIV Serum 0.5 ml 5wds As above Non Rt-PCR
chain 50 IU/ml the virus
Above the cutting
nd Cold Detection of activity of
131 Influenza Oroph. Swab+ Serum 1.5 ml 2 day As above Non threshold ( Rt-PCR
chain the virus
Qualitative)
transpor
tation
Patient
Min. and
quantity storage
n
conditio
ns
Above the cutting Detection of activity of
Oroph. Swab+ Cold
132 Coronavirus 1.5 ml Same day As above Non threshold ( Rt-PCR the virus
Sputum chain
Qualitative)
Above the cutting
Cold Non Detection of activity of
133 RT-PCR Malaria serum 1.5 ml 2 days As above threshold ( Rt-PCR
chain the virus
Qualitative)
134 RT-PCR West Nile
RT-PCR
135
chikungunya
136 RT-PCR Mumps
137 RT-PCR Rift valley
138 RT-PCR Dengue
RT-PCR Above the cutting
Cold Detection of activity of
139 Respiratory panel Oroph. Swab+ Serum 1 ml 3 days As above Non threshold ( Rt-PCR
chain the virus
1 Qualitative)
RT-PCR
140
Respiratory RV16
Above the cutting
RT-PCR Gastro Detection of activity of
141 Stool -- 5 days As above Non threshold ( Rt-PCR
intestinal panel the virus
Qualitative)
RT-PCR Cold Positive band vs Detection of activity of
142 CSF 1 ml 7 days As above Non Rt-PCR
Meningitis chain gel pos marker the virus
Above the cutting
Cold Non Detection of activity of
143 RT-PCR Measles Swab 1 ml 3 days As above threshold ( Rt-PCR
chain the virus
Qualitative)
144 RT-PCR Rubella
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Ministry of Health Issue No.

Ministry of Health& Population

Lab service manual

Dr. SafenazGhareb Dr. Dina Ramadan Dr. Ahmed Safwat

2 LABORATORY REQUEST FORMS/ SPECIMEN CONTAINERS 4

3 IDENTIFYING THE PATIENT: 5

4 LABELLING THE SAMPLE 8

5 SPECIFIC REQUIREMENTS FOR SAMPLES COLLECTION 8

6 TRANSPORTATION & STORAGE OF SAMPLES 9

7 REPORTING OF TEST RESULT 9

11 lists of available laboratory examination 11

5- Quality manager: Dr. Dina Ramadan Lithy

2. Laboratory request forms/ specimen containers:

3. Identifying the patient:

2. Information provided to patients in relation to their own preparation

‫‪Detailed information on sample collection requirements‬‬

‫‪ . 4‬خطوات إستقبال العمالء إلجراء التحاليل الطبية‬

‫‪:4.2‬انواع المعامالت ‪-:‬‬

‫‪:4.3‬غرف سحب العينات‪-:‬‬

‫‪:4.4‬غرفة استالم العينات الخارجية‪-:‬‬

4. Labeling the samples:

5. Specific requirements for samples collection:

2.General rules for sample acceptance & rejection

 Broken or leaking tube/container.

 Insufficient /incorrect details concerning to labeling or to request form.

 Sample collected in wrong tube.

 Request form and sample label do not match

 Inadequate sample type.

 Inadequate sample volume.

 Poor handling during transportation.

 Absence of swabs (for Influenza or corona samples).

 Absence of VTM (for Influenza or corona samples).

 Prolonged transport time &poor handling during transport

3. Receipt of Mislabeled or Unlabeled samples:

4. Time needed between collection and received by the laboratory (transport

5.Disposal of waste material used in specimen collection

6.Transportation and Storage of samples

7. Reporting of test results:

11-lists of available laboratory examination

Culture, Culture on plates

Alanine unlabeled No special Men up to Diagnosis and

Its primary use is in

Stool analysis No special Parasite-Fasciola –

Creatinine 0.5ml serum + R. Assessment of kidney

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