Supervisor, Hematology Department Chief Medical Technologist Designate Head, Laboratory Department

Republic of the Philippines Effectivity Date: Page
Department of Health Nov. 28, 2013 1 of 27

Cotabato Regional and Medical Center
Laboratory Department
Level II: Policy and Procedure Doc. Code:
Service: Ancillary Revision Revision
Process Owner: Hematology Section New No.
Title: Blood Collection Supersedes:
Purpose:
To establish and document policy and procedures for blood collection.
Scope:
This document covers the performance of blood collection starting from the receipt of request
until the delivery of blood samples to their respective sections.
Policy:
The laboratory department ensures that all patients with blood request for blood collection shall
be done by Medical Technologist on duty following the right procedure in performing such test.
Procedure:
The Medical Technologist on duty shall:
1. Prepare kit for blood collection.

2. Do handwashing.
3. Validate Clinical laboratory request form data with the patient
4. Wear Personal Protective Equipment
5. Perform blood extraction.
6. Deliver blood samples to respective sections.
Prepared by: Reviewed by: Approved by:
Venus S. Mendoza, RMT Priscilla E. Francisco, RMT Nena C. Salcedo-Lingayon, MD, FPSP
Supervisor, Hematology Department Chief Medical Technologist Designate Head, Laboratory Department
Level II: Work Instruction Doc. Code:
Process Owner: Hematology section New No.
Title: Blood Collection Supersedes:
Purpose: To provide instructions on blood collection.
Scope: This document covers the processes involved in blood collection.
Work Instruction:
1. Wash your hands with soap and water; then towel dry. Alcohol-based hand sanitizer may
be used generously.
2. Prepare all collection tubes needed and the appropriate syringe/s and needle/s
3. Check for completeness of data on request form
a. Requested tests
b. Patient information
c. Any special requirements
4. Review the request of the patient to determine the order of draw and the use of
appropriate collection tubes and syringe/s with needle.
5. Reassure and position the patient accordingly as you select the appropriate site.
6. Wear disposable gloves and start collecting the required blood specimen accordingly
a. Older children and adults
i. Identify the patient by asking for full name and birth date.
1. Double check the name through the patient’s wristband where
applicable
2. Validate name given with that written on the request form.
ii. Place tourniquet above the site, palpate and locate the vein
1. Disinfect the site with 70% isopropyl alcohol
2. Let the disinfectant evaporate
3. Do not repalpitate the puncture site
iii. Perform the venipuncture. Always follow the correct order of draw
recommended by CLSI Refer to
iv. Collect the specimen into the appropriate container up to the required test
tube marking.
v. Make 2 smears for CBC request.
vi. Remove tourniquet and withdraw the needle from the puncture site.
vii. Apply dry cotton ball over the puncture site and press the site for three
minutes or until the bleeding stops, then apply the adhesive tape over the
puncture site. Never ask the patient to bend the arm because of the risk of
hematoma formation.
viii. Assess for specimen acceptance or rejection. Identify the need to repeat
specimen collection, if necessary.
ix. Label the collection tubes at the bedside or collection area with:
1. Full name of patient.
2. Date of birth of patient.
3. Date and time of collection.
4. Initials of the phlebotomist.
x. Dispose of contaminated material into appropriate waste receptacles
xi. Remove and properly discard gloves
xii. Wash and dry your hands after collection
xiii. Promptly send the specimens with the completely filled-out request form
to the laboratory.
b. Newborn and infants
i. Identify the patient by asking for full name and birth date.
1. Double check the name through the patient’s wristband where
applicable
2. Validate name given with that written on the request form.
ii. Massage gently the heel to help increase blood flow
iii. Clean the site to be punctured with an alcohol swab. Dry the cleaned area
with a dry cotton ball. Hold the baby’s foot firmly to avoid sudden
movement.
iv. Using a sterile lancet, puncture the side of the heel.
v. Wipe away the first drop of blood with a piece of clean, dry cotton. Use
gentle pressure to produce a rounded drop of blood
vi. Fill the capillary tube/s or micro collection device/s only up to the mark
indicated.
vii. Make 2 smears for CBC request
viii. After collection, elevate the heel, place a piece of clean, dry cotton on the
puncture site, and hold it in place until the bleeding has stopped. Refer to
physician if bleeding does not stop.
ix. Label the collection tubes at the bedside or collection area with:
1. Full name of patient.
2. Date of birth of patient.
3. Date and time of collection.
4. Initials of the phlebotomist.
x. Dispose of contaminated material into appropriate waste receptacles
xi. Remove and properly discard gloves
xii. Wash and dry your hands after collection
xiii. Promptly send the specimens with the completely filled-out request form
to the laboratory.
Republic of the Philippines Effectivity Date: Page:

Department of Health November 28, 2013 5 of 27
Title: Complete Blood Count Supersedes:
Purpose: To established a documented policy and procedure for CBC at Hematology Section
Scope: This document covers all assigned Medical Technologists at Hematology section
involved in performing CBC from receipt of the sample to the release of the result.
Policy: The laboratory department shall ensure that all samples for CBC shall not be performed
without completing or accomplishing CBC request form.
Procedure:
The Medical Technologist shall:
1. Check the label of the tube and compare it with the name written in the request.
2. Check the correct volume requirement.
3. Check for the presence of clot in the sample.
4. Arrange the sample in the rack.
5. Load to the Analyzer.
6. Wait until the machine finish the process.
7. Check results in the machine.
8. Validate result.
9. Print and sign result.
10. Release result.

Department of Health 6 of 27
Level III: Work Instruction Doc. Code:
Title: Complete Blood Count Supersedes:
Purpose: To establish a documented work instruction for Complete Blood Count.
Scope: This document covers all Medical Technologists involved in performing CBC from
receipt of sample to release of result.
Work Instructions
CBC -Using XT-1800i Analyzer
The Medical Technologist on shall:
1. Received sample from the Phlebotomist

1.1 Check the volume required
1.2 Check for the presence of clot
1.3 Check the label of the tube with the name in the request
2. Load the sample to the rack

2.1 Load the rack to the machine
2.2 Click “sampler” in the machine
3. Wait till machine finish processing the samples

4. Encode name/data of patient according to arrangement in the rack
5. View results, click “completed”, click “name of patient” on the machine.
5.1 For additional action after doing an automated CBC refer to Table 1 page 14-
15 of the Handbook PCQACL’s Standardization and Harmonization of Complete
Blood Count in the Philippines 2012
5.2 Follow instructions given in the handbook
5.3 Do necessary corrections/validations
6. Approve results
7. Print results
8. Sign the result and stamp the facsimile of the Pathologist
9. Record result in the corresponding logbook
10. Endorse result to the laboratory aide in charge for passing out to the wards.
11. Endorse out-patient result to the encoder for release
CBC - using HMX Coulter
1. Receive samples from the Phlebotomist

2. Arrange the samples in the rack
2.1 Give the rack to the LIS encoder for assigning of barcode stickers and encoding of
patients identification
3. Load the rack with the sample to the analyzer.
4. Click “run samples”
5. Wait until the machine finish processing
6. View results in the computer
6.1 For additional action after doing an automated CBC refer to Table 1 page 14-15
of the Handbook PCQACL’s Standardization and Harmonization of Complete Blood
Count in the Philippines 2012
7. Approve result
8. Print result
9. Sign the result and stamp the facsimile of the Pathologist
10. Record the result in the corresponding logbook
12. Endorse out-patient result for release
CBC - 0.25 to 0.5 ml volume sample using XT-1800i Analyzer
1. Receive samples from the Phlebotomist

2. Arrange the sample in the rack
3. Click “manual menu” in the machine
4. Encode patients’ identification in the machine
5. Aspirate sample on the probe
6. Wait until machine finish processing the sample.
7. Encode complete data of patient.
8. View results in the computer
8.1 For additional action after doing an automated CBC refer to Table 1 page 14-
15 of the Handbook PCQACL’s Standardization and Harmonization of Complete
Blood Count in the Philippines 2012
9. Approve result
10. Print result
11. Sign the results and stamp the facsimile of the Pathologist
12. Record the result in the corresponding logbook
14. Endorse out-patient result for release

Title: Reticulocyte Count Supersedes:
Purpose: To establish a documented Working Instruction for Reticulocyte counting at the

Laboratory Department.
Scope: This document covers all Medical Technologists assigned at the Hematology Section
involved in performing Reticulocyte count.
Work Instruction:

1. Match name of patient in the request with the label of the tube.
2. Check sample for presence of clot.
3. Prepare and label test tube with patient’s identification.
4. Mix sample by inversion 4-6 times.
5. Transfer 100ul of sample and mix with 100ul of Brilliant Cresyl blue reagent in a test
tube.
6. Mix and let it stand for 30 minutes.
7. Make 2 smears and let it dry.
8. Examine smear under oil immersion objective.
9. Count Reticulocytes seen in 500 RBC’s or count reticulocyte in 10 consecutive fields.
Use only the area where there is an even distribution of red cells.
10. Record result in the request.
11. Encode result in the computer.
12. Record result in the corresponding logbook.
13. Endorse in-patient result to the laboratory aide in charge for passing out in the wards.
14. Endorse out-patient result for release to the encoder.

Title: Bleeding Time Supersedes:
Purpose : To establish a documented Working Instruction for Bleeding Time at the laboratory
Scope: This document covers all Medical Technologists assigned at the Hematology section
involved in performing Bleeding Time from receipt of request to releasing of result
Work Instruction:
1. Accept and check the completeness of the request.

2. Go to respective wards as indicated in the request.
3. Call the name of the patient.
4. Go to the bedside of the patient and verify patient identification tag.
5. Explain the procedure to be done.
6. Wipe the finger of choice with cotton soaked with alcohol and then followed by
dry cotton.
7. Prick the finger.
8. Wipe the punctured area of the finger with dry cotton.
9. Allow the wound to bleed freely.
10. Start timer in seconds, using a stopwatch.
a. Blot every after fifteen seconds using filter paper
11. Record the time of last blot where no more traces of blood can be seen.
12. Write result in the request.
13. Encode official result in the computer, check and sign the result.
14. Stamp the facsimile of the pathologist.
16. Endorse result to the laboratory aide for passing out to the wards.
For out-patient:

2. Go to out-patient extraction area
4. Let the patient sit down.
5. Reconfirm name of the patient
7. Wipe the finger of choice with cotton soaked with alcohol and then followed
by dry cotton.
9. Wipe the punctured area of the finger with dry cotton.
a. Blot every after fifteen seconds using filter paper
12. Record the time of last blot where no more traces of blood can be seen.
13. Write result in the request.
17. Endorse out-patient result to the encoder for release.

Title: Clotting Time Supersedes:
Purpose: To establish a documented Work Instructions for Clotting Time
Scope: This document covers all Medical Technologists involved in performing Clotting Time
starting from receipt of the request to the releasing of result.
Work Instruction:
1. Check the completeness of the request.
2. Go to respective wards as indicated in the request.
3. Call the name of patient.
4. Go at the bedside of the patient and verify patient identification tag.
6. Wipe the finger of choice with cotton soaked with alcohol and then followed by dry
cotton.
8. Collect one drop of blood to the slide.
9. Start timer in seconds using stopwatch.
10. Examine for clot formation every after 15 seconds until fibrin is formed by fishing
out the blood drop with the use of lancet.
11. Record the result in the request.
12. Encode the result in the computer.
13. Check and sign the result.
15. Record the result in the corresponding logbook.
16. Endorse in-patient’s result to the laboratory aide in charge for passing out in the
ward.
For out –patient

2. Go to out-patient extraction area
4. Let the patient sit down.
5. Reconfirm name of the patient
7. Wipe the finger of choice with cotton soaked with alcohol and then
followed by dry cotton.
9. Collect one drop of blood to the slide.
12. Examine for clot formation every after 15 seconds until fibrin is formed by
fishing out the blood drop with the use of lancet.
13. Record the result in the request.

Title: Erythrocyte Sedimentation Rate Supersedes:
Purpose : To establish a documented Work Instruction for ESR at the laboratory
Scope : This document covers all Medical Technologists involved in performing Erythrocyte
Sedimentation Rate.
Work Instruction:

1. Countercheck name of patient in the request with the label in the tube.
2. Check sample for presence of clot.
3. Balance the ESR rack or stand by adjusting the screw until the marker matches or is
located at the center.
4. Mix sample by gentle inversion for 4-6 times.
5. Transfer sample in the Wintrobe tube using Pasteur pipette
6. Stand the tube for one hour.
7. Read result
8. Record result in the request
9. Encode or make official result and sign
10. Stamp
11. Record result in the Hematology logbook
12. Endorse In patient result to the clerk in charge for passing out in the wards.
13. Release result after 2 hours for Out- patient.

Process Owner: Medical Technologist New No.
Title: Internal Quality Control for Coulter, Supersedes:
HMX and SYSMEX 1800i Analyzers
Purpose: To establish documented working instructions for daily running of Three levels of
control for Coulter HMX and Sysmex analyzers.
Scope: This document covers all Medical Technologists assigned at the Hematology Section in
the Morning shift involved in running of daily controls for the two Hematology
Analyzers.
Policy: The Laboratory Department ensures that daily running of controls shall be done before
the start of the morning shift following the manufacturer’s test methodology.
Procedure:
Coulter HMX
1. Press "ESCAPE"
2. Press F2 Run Controls
3. Press F2 File
4. Select level of Controls to be run then press "Enter"
5. Load the Controls to the rack then load to the machine
6. Press F3 run
7. Wait for the result
7.1 Compare result with the manufacturer's insert
7.2 If results are out of range, do the following:
7.2.1 Check the maintenance/cleaning of the machine
7.2.2 Re-run the controls
7.2.3 If results are still out of range call Service Engineer
7.2.4 If results are within the range, analyzers are ready for use.
8. Print and file results of the controls.
Sysmex XT 1800i
1. Click Menu
2. Click QC Analysis
3. Select Level of Control to be run ( Level 1,Level 2 and Level 3)
4. Press Ok
5. Sip the Control at the probe and press the start switch
6. Wait for the result to come out and compare result with the manufacturer's insert
7. Press Accept if the result is within the range
7.1. If results are out of range, do the following:
7.2 Check the maintenance/cleaning of the machine
7.3 Re-run the controls
7.4 If results are still out of range call Service Engineer
7.5 If results are within the range, analyzers are ready for use.

Title: Internal Quality Control Supersedes:
Purpose: To establish a documented working instructions for reagent preparation and daily
running of controls at the laboratory.
involved in reagent preparation and running of daily controls.
Policy: The Laboratory Department ensures that daily running of control shall be done before the
start of the morning shift and reagent preparation following the right procedure.
Procedure:
I. Reagent Preparation:
A. PT-FIBRINOGEN HS PLUS
1. Follow the product insert for reagent reconstitution.
1.1 Bring out from the refrigerator reagents to be reconstituted.
1.2 Check label and expiry date of the reagents.
1.3 Maintain reagents at room temperature for 30 minutes.
2. Dissolve the contents of each vial of Thromboplastin by pouring the entire contents of
one vial of buffer into the reagent vial.
3. Replace the stopper and swirl gently.
3.1 Do not try to pipette the exact volume required for reconstitution of
Thromboplastin reagent.
3.2 Make sure of the complete reconstitution of the product.
3.3 Keep the reagent at 15-25̊ C for 30 minutes.
3.4 Invert gently to mix before use.
3.5 Do not shake.

4. Keep the reconstituted reagent in its original bottle at 2-8̊C when not in use. Reagent is stable
for 5 days.
5. Empty the Thromboplastin reagent into reservoir number 1- PT- FIB of the instrument.
6. Empty the cephalin reagent into reservoir 2 (APTT-SP), and the calcium chloride reagent
into reservoir number 3 CaCl 2 on the instrument.
B. NORMAL CONTROL PLASMA
1. Bring out from the refrigerator the Normal control plasma to be reconstituted
1.1 Check label and expiration date.
1.2 Maintain the control at room temperature for 30 minutes.
2. Dissolve the contents of the vial by adding 1 ml of distilled water.
2.1 Replace the stopper and swirl gently by doing the figure of eight.
2.2 Keep the reagent at 15-25̊C for 30 minutes before using.
II. RUNNING OF DAILY CONTROL
1. Perform "PRIMING" on the machine for three times.
2. Empty the Thromboplastin reagent into reservoir number 1 (PT- FIB) of the
instrument.
3. Empty the cephalin reagent into reservoir 2 (APTT-SP), and the calcium chloride
reagent into reservoir number 3 CaCl2 on the instrument.
4.Proceed to Test
5. Select PT-APTT, then Enter
6. Press start analysis
7. Select Load list, press arrow up, then enter

8. Press delete, to erase previous name of patients.
9. Encode Normal Control, then enter
10. Press "COMMANDS"
11. Enter Rotor number, then enter
12. Wait until machine finish the processes and give result
13. Check the control result, it be within the specific SD limit listed below.
Procedure for normal control range:
1. Normal control level shall be within ±6 of the calibrated normal control
2. If control result is outside ±6 perform troubleshooting
2.1. check reagent levels and expiration dates.
2.1.2 check instrument maintenance/cleaning.
2.1.3. re run the control.
3.Reconstitute new control if result is still outside the range.
4. Notify the Hematology Supervisor immediately if result is still outside the limits.
Department of Health November 28, 20 of 27
Cotabato Regional and Medical Center 2013
Process Owner: Hematology New No.
Title: Prothrombin Time and Activated Partial Supersedes:
Thromboplastin Time
Purpose: To establish a documented policy and procedures for PT and APTT at the Laboratory
Department.
involved in performing PT and APTT, from receipt of sample to release of result.
Policy: The Laboratory Department ensures that all samples for PT and APTT shall strictly
follow the volume requirement of the test following the proper procedure. Test shall be
done within one hour after extraction.
Procedures:
1. Check and match name of the patient with the label on the tube.
2. Check the volume of the sample.
3. Centrifuge the sample as soon as possible. Plasma shall be free from hemolysis. It shall
neither be lipemic nor icteric.
4. Make sure Reference Emulsion level is sufficient enough.
5. Perform "priming" on the machine.
6. Transfer reagents needed for PT and APTT to its reservoirs.
7. Run Normal Control plasma every start of the day for PT and APTT in the same manner as
patient sample. Check the control results; it shall be within the specified SD limits.
8. Perform the test within one hour after extraction.
9. Release the result two hours after extraction.
Title: Prothrombin Time and Activated Partial Supersedes:
Thromboplastin Time
Purpose : To establish a documented working instruction for PT and APTT at the Laboratory
Department.
Scope : This document covers all medical technologists assigned at the Hematology section
involved in performing PT and APTT.
Work Instructions:
1. Receive samples from the phlebotomist.

2. Match the name of the patient with the label of the tube.
2.1 Check for the presence of the clot.
2.2 Check the volume of the sample.
3. Perform “ priming ” on the machine
4. Centrifuge the sample as soon as possible for 20 minutes at 3500 revolution per
minute.
4.1 Check the citrated plasma for hemolysis, lipemia and icterus.
4.2 Defer the sample if plasma is hemolyze, lipemic or icteric.
4.4 Return the request to the phlebotomist in charge for re-extraction.
4.5 Inform Nurse on duty.
5. Get the reagents from the refrigerator.

5.1 Allow the reagents to stand at room temperature for 30 minutes.
5.2 Transfer the reagents from the reagents bottle to its receptacle in the machine.
6. Transfer 150ul of Citrated plasma to the sample cup.
7. Load the sample to the machine to the sample tray numbered of the instrument
8. Wait for 10seconds.
9. Press “test” on the machine.
10. Select test desired.
11. Press “start analysis arrow”.
12. Encode the name of the patient on the load list
13. Press the rotor number
14. Wait for 4 minutes while the machine reads the sample
15. Read result given by the machine
16. Encode result in the computer
18. Endorse in-patient result to the laboratory aide in-charge for passing-out in the
wards.

Title: Peripheral Blood Smear Supersedes:
Purpose : To establish a documented Work Instruction for Peripheral Blood Smear
Scope : This document covers all Medical Technologists assigned at the Hematology Section
involved in the preparation of smear and the pathologist for reading until release of result.
Work Instruction:
1. Accept smears from the phlebotomist.
2. Check label of the smears with the request.
3. Stain the smear
3.1 Fix the smear by dipping with methanol for 5 times.
3.2 Transfer slide to Eosin by dipping 5 times.
3.3 Transfer slide to Methylthionin by dipping 5 times.
3.4 Wash briefly in tap water.
3.5 Allow to dry.
4. Place the stained smear on top of the request
5. Attach CBC result of the patient.
The Chief Medical Technologist designate shall:

1. Assign accession number to both the smear and request
2. Bring stained smear to the office of the pathologist for reading
3. Endorse one copy of the result to the laboratory aide for pass out to in patient
4. Endorse result to the encoder for out patient
The Pathologist shall:

1. Read the stained smear in the microscope
2. Encode and sign the result
Service/Dept/Section: Ancillary Revision Revision
Process Owner: Medical Technologist New No.
Title: Quality Assurance Supersedes:
Purpose:
To establish a documented policy and procedures of at the Hematology section on Quality

Assurance.
Scope:
This document covers procedure for Quality Control starting from daily maintenance of the
analyzers to daily running of Controls by the Medical Technologist assigned at the Hematology
Section.
Policy:
The Hematology Section shall follow the manufacturer's test methodology and operator's manual
of analyzer.
Procedure:
1. Perform cleaning of Hematology Analyzers every start of each shift.

2. Check the volume contents of all the reagents of the analyzers
3. Perform daily quality controls before the start of the Morning shift.
4. Compare results with the insert given by the manufacturer.
4.1 If results are out of range, do the following:
4.1.1 Check the maintenance/cleaning of the machine
4.1.2 Re-run the controls
4.1.3 If results are still out of range call Service Engineer
4.1.4 If results are within the range, analyzers are ready for use.
Venus S. Mendoza, Priscilla E. Francisco, Nena C. Salcedo-Lingayon, MD,

RMT RMT FPSP
Supervisor, Hematology Chief Medical Technologist Head, Laboratory Department
Department Designate
Title: Specimen and Document Retention Supersedes:
Purpose: To establish a documented policy and procedure on specimen and document retention.
Scope: This document covers processes on specimen and document retention by the Medical
Technologist on duty at Hematology Section.
Policy: The Laboratory Department shall ensure that guidelines on specimen and document
retention processes are strictly adhered to.
Procedure:
1. Retain properly labeled hematology slides for one week.
2. File blood smears by date and wrapped in clean paper before storing at the Hematology
Section.
3. Dispose blood smears after one week.
4. Retain blood samples collected in EDTA tubes which maybe used for re-testing within 2 hours
after collection if keep in room temperature of 22-24̊C and for 24 hours if keep in the refrigerator
at 4-8̊C.
5. Keep hematology records for at least one year.
Title: Storage of samples Supersedes:
Purpose: to establish a documented policy and procedure for the storage of hematologic sample
after examination.
Scope: This document covers the Medical Technologist assigned at the Hematology section
involved in storing of hematology samples
Policy: The laboratory department shall consistently implement the storage of all hematology
samples for 48 hours at a temperature of 6-8o C by the medical technologist on duty.
Procedure:
1. Arrange all samples in a tray.

2. Label the tray with date and time of shift
3. Keep or place the sample in the reagent refrigerator for 48 hours.
Service/Dept: Ancillary Revision Revision
Title: Alert Values Reporting Supersedes:
Purpose: To establish a documented policy and procedure on reporting alert values to physician
after referral to pathologist.
Scope: This document covers reporting of alert values by the Medical Technologist on duty to
physician that may be clinically significant if not acted upon immediately.
Policy: The laboratory department ensures that alert values are reported to clinicians as soon as
possible by the Medical Technologist on duty after referral to the pathologist.
Procedure:
The Medical Technologist on shall:
1. Refer to the pathologist when alert value is detected.

2. Inform the physician about the alert value encountered after reviewed by the pathologist
3. Record alert value in the logbook

Supervisor, Hematology Department Chief Medical Technologist Designate Head, Laboratory Department

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Supervisor, Hematology Department Chief Medical Technologist Designate Head, Laboratory Department

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Republic of the Philippines Effectivity Date: Page

Department of Health Nov. 28, 2013 1 of 27

1. Prepare kit for blood collection.

Prepared by: Reviewed by: Approved by:

Purpose: To provide instructions on blood collection.

Scope: This document covers the processes involved in blood collection.

The Medical Technologist on duty shall:

Prepared by: Reviewed by: Approved by:

Republic of the Philippines Effectivity Date: Page:

Prepared by: Reviewed by: Approved by:

Republic of the Philippines Effectivity Date: Page:

Purpose: To establish a documented work instruction for Complete Blood Count.

CBC -Using XT-1800i Analyzer

The Medical Technologist on shall:

1. Received sample from the Phlebotomist

2. Load the sample to the rack

3. Wait till machine finish processing the samples

CBC - using HMX Coulter

The Medical Technologist shall:

1. Receive samples from the Phlebotomist

CBC - 0.25 to 0.5 ml volume sample using XT-1800i Analyzer

The Medical Technologist shall:

1. Receive samples from the Phlebotomist

Prepared by: Reviewed by: Approved by:

Republic of the Philippines Effectivity Date: Page:

Purpose: To establish a documented Working Instruction for Reticulocyte counting at the

The Medical Technologist shall:

Prepared by: Reviewed by: Approved by:

Republic of the Philippines Effectivity Date: Page:

The Medical Technologist shall:

1. Accept and check the completeness of the request.

1. Accept and check the completeness of the request.

Prepared by: Reviewed by: Approved by:

Republic of the Philippines Effectivity Date: Page:

For out –patient

1. Accept and check the completeness of the request.

Prepared by: Reviewed by: Approved by:

Republic of the Philippines Effectivity Date: Page:

The Medical Technologist shall:

Prepared by: Reviewed by: Approved by:

Republic of the Philippines Effectivity Date: Page:

The Medical Technologist shall:

7.2 If results are out of range, do the following:

7.2.1 Check the maintenance/cleaning of the machine

7.2.2 Re-run the controls

7.2.3 If results are still out of range call Service Engineer

8. Print and file results of the controls.

7.2 Check the maintenance/cleaning of the machine

7.3 Re-run the controls

7.4 If results are still out of range call Service Engineer

8. Print and file results of the controls.

Republic of the Philippines Effectivity Date: Page:

The Medical Technologist shall:

1. Follow the product insert for reagent reconstitution.

1.1 Bring out from the refrigerator reagents to be reconstituted.

1.2 Check label and expiry date of the reagents.

1.3 Maintain reagents at room temperature for 30 minutes.

3. Replace the stopper and swirl gently.

3.2 Make sure of the complete reconstitution of the product.

3.3 Keep the reagent at 15-25̊ C for 30 minutes.

3.4 Invert gently to mix before use.

3.5 Do not shake.

into reservoir number 3 CaCl 2 on the instrument.