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Biology is the study of the life and its interaction with its
environment. What life is and the technology needed to study life
form the basis of this unit.
Science relates the human condition. The choices we make are not
just based on knowledge, facts, and number; they are evaluated in
terms of what is important to us and our acceptance of risk.
9. Plants p. 193
Carl Sagan
Advancements in technology such as the microscope, was necessary to better understand the basis of life
and the cell.
/ Notes:
Life can only come from other life. The ancient idea that life can
occur without previous life, called spontaneous generation, did not
stand up to scientific experiments, methods, and equipment such
as the microscope. The microscope lead to the discovery of the
cell and the parts that make it up.
1. Identify the idea that cells can be produced from non-living substances.
Problems
1. The idea of spontaneous __________________ says life can arise from non-living things but
modern biology states that life can only come from other _______________. The basis for all life is
the __________.
Francesco Redi was an Italian physician and poet and is often considered to be the founder of
experimental biology. In 1668 he published the findings of his experiments on spontaneous generation in
which he used jars containing meat. Some jars were covered with gauze that allowed airflow and some
were left uncovered. Flies couldn’t enter the covered jars but were observed to enter the uncovered jars
and only the meat in the uncovered jars grew maggots.
2. Spontaneous generation (aka abiogenesis) is the idea that life can be produced from non-living
matter. Was Redi’s experiment sufficient to disprove the idea of spontaneous generation? Explain.
3. Redi contributed to the modern scientific methods by introducing the importance of the controlled
variable. List two of Redi’s controlled variables.
Louis Pasteur conducted controlled experiments to test the idea of spontaneous generation. He tested if
life could start from yeast broth and air. He designed a container with an S-shaped opening that allowed air
to enter but no bugs or dust. The yeast broths in the containers were first boiled to kill any
microorganisms already present.
Yeast broth
4. What might have Pasteur concluded from 5. Identify Pasteur’s manipulated variable.
his observations?
8. Louis Pasteur introduced the concept of “pasteurization” where yeast broths were heated to kill all
microorganisms. Why are milks, cheese and yogurts now pasteurized before they are sold in stores?
Observation often leads to theory but direct observation is often not sufficient. A theoretical basis for
biogenesis relied on improvements in technology. Sometimes a theory cannot be tested without the
availability of specialized technology. Robert Hooke examined thin slices of cork through a microscope he
constructed with a magnification power of 30X and noticed small room like compartments he called cells.
9. State the three points of the cell theory. 10. Was Hooke’s observations enough to propose
the cell theory?
11. Is it possible to support the 12. Viruses are smaller than cells and do not
a. theory of biogenesis (that life comes contain a nucleus. They reproduce by invading
from life) without the development of and destroying cells. Some scientists consider
the microscope? them to be alive even though this would
b. cell theory without the development of contradict the cell theory of life. Are exceptions
the microscope? to a theory allowed? Explain.
13. How might an experiment be done to show that cells come from pre-existing cells?
14. Over 2300 years ago Aristotle classified animal life and attempted to explain how life is propagated.
He suggested that “each life springs from a definite parent and gives rise to a predictable progeny.”
How might Aristotle’s statement be related to the cell theory?
The study of the cell would have been impossible without the development of the microscope.
Microscopy still plays a key role not only in the study of cells but many other areas of biology and science.
eye piece
tube
nose piece
objective lenses
arm
stage
diaphram
coarse focus
fine focus
illuminator
base
Magnification is how many times larger the image is than the object:
hi
M=
ho
Where:
M is magnification ratio (no units)
hi is height or length of the image Any units as long as
ho is height or length of the object they are consistent
Multiply by
cm 10 000 µm
C
o
mm 1000 µm m
m 100 cm p
cm 10 mm o
u
Divide by n
d microscopes have at least two lenses.
Mtot = Me x Mo
Where:
Mtot is the total magnification
Me is the magnification of the eyepiece (ocular) lens
Mo is the magnification of the objective lens
Field Diameter (or field of view) is the distance across the field
of view. As magnification increases the field diameter decreases.
M H FH = M L FL
Where:
M is magnification ratio (no units) H - high
F is the field of view or field diameter (fov) L - low
Wavelength is the
length of one repeating
Resolution is the ability to distinguish detail; to see different objects. unit of a wave
Techniques such as staining make it possible to better view samples under a microscope. If a sample is
transparent it will not be easy to see under a microscope. Adding a stain will increase the contrast between
different parts of the sample making it more visible.
1. A 1.4 mm long specimen appears to have a length of 46.3 mm through a microscope lens. Calculate
the magnification power of the microscope.
2. A student uses a microscope that has a low power magnification of 5.0X and a high power
magnification of 40X. The high power field of view is 5.0 mm. Determine the field of view for the
low power magnification.
Problems
1. Cells can be studied using ____________ to magnify the tiny cells. A _____________ microscope has
at least __________ lenses. The overall magnification is the magnification power of the eye-piece
multiplied by the magnification power of the ____________ lens.
2. The letter “e” is cut from paper and 3. Early microscopes used a single lens and were
placed under a microscope and the called “flea glasses” as they were used to look at
image produced is shown below. How insects. A 1.2 mm long insect appears to have a
does the microscope affect the image? length of 12.1 mm through a microscope lens.
Calculate the magnification power of the
microscope.
8. A 200 µm long specimen is viewed under a 9. The best optical microscopes can magnify up
250X microscope. Calculate the length of the to 1500X. Calculate the size of an object in
image in units of cm. units of micrometres, if its image size is 2.0 mm
with a magnification of 400X.
Microbiology is the study of microbes. In 1674 a Dutch cloth maker, Anton van Leeuwenhoek, used a
simple microscope (single lens) that was superior to previously constructed compound microscopes to
observe bacteria and microorganisms in pond water. He is often called the father of microbiology and
obtained magnifications over 270X. He said
… there were many very little living animalcules, very prettily a-moving. The biggest sort . . . had a
very strong and swift motion, and shot through the water (or spittle) like a pike does through the
water. The second sort . . . oft-times spun round like a top . . . and these were far more in number.
11. An overall magnification can be determined using the magnification power of the eyepiece and the
magnification power of the objective lens in a compound microscope (Mtot = Me x Mo). Complete
the chart below.
12. In 1595 a Dutch spectacle maker, Zacharias Janssen and his father Hans, constructed the first
compound microscope having a magnification power of 9.0X. Assuming an objective lens power of
2.0X, determine the power of the eyepiece.
In 1665 Robert Hooke constructed his own compound microscope and used it to study many small
animals and noticed what he called cells in a sample of cork. Hooke wrote that
… the collection of most of which I made use of microscopes and some other glasses and
instruments that improve the senses . . . only to promote the use of mechanical helps for the senses,
both in the surveying the already visible World, and for the discovery of many others hitherto
unknown.
13. One of Hooke’s microscope consisted of two lens, one with a power of 5.0X and the other with a
power of 6.0X. Determine the overall magnification of Hooke’s microscope.
A millimetre ruler may be used to determine sample size. The ruler is place over top the slide and is viewed
through the microscope along with the sample.
15. Two samples, human hair and a bee’s wing, are viewed with a mm ruler under a 40X microscope.
Estimate the maximum width of each sample.
Hair
b. wing
16. Magnification and field of view are related to each other; as magnification increases, the field of view
decreases. Complete the chart below.
Low Power High Power
Magnification Field of view (mm) Magnification Field of view (mm)
a. 20X 5.0 40X
b. 40X 8.0 3.2
c. 10X 500X 0.24
18. A compound microscope has a low power magnification of 6.0X and a high power magnification of
40X. The high power field of view is 5.0 mm. Determine the field of view for the low power
magnification.
19. A compound microscope has a high power magnification of 100X and a low power magnification
of 40X. The low power field of view is 5.0 mm. Determine the field of view for the high power
magnification.
20. A compound microscope has a high power field of view of 4.00 mm and a low power magnification
of 40.0X. The low power field of view is 10.0 mm. Determine the magnification for the high power.
21. Three important factors required to view a microscopic are magnification, resolution, and contrast.
What technique may be used to improve contrast in a cell specimen?
For more detailed observation of the cell’s structure and process more powerful microscopes were
developed. The electron microscope allowed observation near the molecular level.
/ Notes:
For an object to be seen or resolved its size must not be
much smaller than the wavelength used. A beam of
electrons has a shorter wavelength than a beam of light.
Thus the smaller wavelength of the electron beam in an
electron microscope can resolve smaller objects.
Example
Problems
Use the terms below to complete the sentence that follow.
Optical resolution is the ability to distinguish between different objects. Simply magnifying without
improving resolution will create a larger blurry object. Greater resolution is required to view smaller
objects. Electrons have smaller wavelengths than visible light so they have higher resolution and are better
for seeing smaller objects.
i
ii
iii
2. Identify the range of objects best suited for 3. For a wave to detect an object, the object
being viewed by an must be at least half the size of the length of
a. electron microscope. the wave. Which wave below (a or b) would
have the best chance of detecting the sample
b. optical microscope.
object shown?
c. unaided human eye. a.
sample
b.
4. List benefits and drawbacks of an optical 5. Deoxyribonucleic acid (DNA) is a large molecule
microscope over an electron microscope. in the nucleus of each cell. It contains the
code/instructions for the development, structure,
and function of all living organisms. Can DNA
be viewed using an optical microscope?
6. Electron beams produce a ____i____ wavelength than visible light. Therefore, electron beams can
resolve ____ii____ objects.
i ii
a. longer larger
b. longer smaller
c. shorter larger
d. shorter smaller
The optical microscope allowed the cell and many of its parts to be / Notes:
observed and their function to be studied. Cells are composed of
many different parts called organelles.
All cells have a cell membrane which contains all the parts of the
cell and selectively allows for substances to enter and leave the cell.
Plant cells also have a cell wall which provides shape and rigidity
to the cell. The organelles are in the cytoplasm, which is the
soapy-like fluid inside the cell. The actions of the cell and how the
cell develops are controlled by the nucleus of the cell. The genetic
code is stored in a DNA molecule inside the nucleus (the largest
organelle).
Plant cells differ from animal cells in that plant cells have cell
walls and chloroplast and animal cells don’t. Chloroplasts are
specialized organelles that contain chlorophyll (which give
plants their green colour) that use light energy to convert water
and carbon dioxide into glucose according to the following
reaction:
lysosome
nucleus
ribosome
cell wall
vacuole, rough, smooth, plant, chloroplast, Golgi, ribosomes, mitochondria, ribosomes, lipids
1. Animal cells do not have a cell wall but ____________ cells do. Plant cells also have a large central
___________ and contain the organelle called ______________ that is responsible for converting
light energy to chemical energy. ________________ are responsible for synthesizing proteins while
the _______________ apparatus processes those proteins. The _______________ process the
energy required by the cell using the ATP molecule. Vesicles that are seen as bubbles or tubes within
the cell contain _______________ and can synthesize proteins and are called ______________
endoplasmic reticulum. The ______________ endoplasmic reticulum synthesizes ____________
and carbohydrates.
2. Even in the modern era of camera technology that interfaces with microscopes, it is still valuable to
hand sketch what is viewed through a microscope; this leads to better observation and recall. Anna
drew the following diagrams of cells she observed through a microscope. Label the parts of the cells.
a.
b. f.
c.
d.
g.
e.
a. nucleus:
b. ribosomes:
e. cell membrane:
f. cytoplasm:
g. mitochondrion:
h. Golgi apparatus:
i. vacuole:
j. lysosomes:
k. chloroplast:
l. cell wall:
Gaucher’s disease is a genetic disease caused by malfunctioning enzyme production by the lysosomes. The
waste products are not properly disposed of resulting in liver and spleen problems and fatigue and easy
bruising.
4. Identify the organelle responsible for removing the waste product from the cell.
A study conducted in the University of Pittsburgh School of Medicine investigated mitochondria levels in
muscle cells. In the study, eight 68 year olds (5 men and 3 women) who were not otherwise active had
their mitochondria levels measured before and after a 12-week exercise program. It was observed that their
mitochondria levels increased by 50 %.
7. Identify two cell features that cannot be viewed using an optical microscope.
8. Alzheimer’s disease affects many older people and is the sixth leading cause of death in those over
65. On a cellular level it is a result of a dysfunction of the cell membrane of neuron cells in the brain.
Apparently the phospholipids molecules of the cell membrane may be affected but no drugs have
yet been developed that provide positive results. List some of the attributes of a well-constructed
drug trial that would be required for a new Alzheimer’s drug. (Note page 30)
A reticle (eyepiece micrometer) is a transparent scale viewed through a microscope superimposed over the
image and can be used to calculate the size of the object being viewed. This method is more accurate than
using a ruler on the sample being viewed. An eyepiece reticle, with divisions in mm, is shown below.
D
d=
0 5.0 10.0 M
Where:
d is the spacing distance (mm)
D is the reticle’s divisions (mm)
M is the objective magnification power
1. Determine the spacing of the scale in millimetres and micrometres. Complete the chart below.
2. A sample, as shown below, is viewed through a microscope at 40X. Determine the size of the sample
if the reticle divisions represent 1.00 mm marks.
0 5.0 10.0
1. Identify the statement below that is NOT a part of the cell theory.
a. All living things are made from cells
b. Cells only come from other cells
c. Cells must be small in order to efficiently transport material in and out
d. Cells are the basic building block of all life
2. A microscope with an eyepiece magnification power of 10X and an objective lens power of 4.0X has
an overall magnification of
a. 0.25X
b. 2.25X
c. 4.0X
d. 40X
3. Convert 400 mg to g.
a. 400 g b. 4.00 g c. 0.400 g d. 0.0400 g
4. Andrew measures a microscope’s field of view to be 4.2 mm when using a low power 40X
magnification. The blood cells he views are too small to be measured. He switches to a 400X
magnification and notes that each blood cell occupies about 20 % of the field of view. The size of
each blood cell is ____ mm.
a. 0.084 b. 0.42 c. 2.1 d. 0.042
6. A 1.4 mm long specimen appears to have a length of 46.3 mm through a microscope lens. The
magnification power of the microscope is
a. 33X
b. 0.030X
c. 30X
d. 65X
7. The ability of a microscope to increase the apparent size of an object is called ___i___ while the
ability for the microscope to distinguish detail is called its ___ii____.
i ii
a. power depth of field
b. resolution power
c. resolution magnification
d. magnification resolution
i ii
a. nucleus cell membrane
b. cell membrane nucleus
c. ribosomes lysosome
d. lysosome ribosomes
9. Energy is distributed throughout the cell via the ATP molecule which is produced in the
a. ribosomes
b. mitochondria
c. lysosomes
d. Golgi apparatus
10. Samples to be viewed using a microscope are often stained so they can be seen better. Staining
improves the
a. magnification of the sample
b. brightness of the sample
c. depth of field possible for different parts of the cell
d. contrast between different parts of the cell
11. As magnification increases, the field of view will ___________. When focusing a microscope it is
best to start with _________ power and then go to __________ power and lastly to _________
power. A compound microscope has a minimum of __________ lenses. Staining a sample may
improve the ___________ between different parts of the sample making it easier to see.
12. Compare (list similarities and differences) between lysosomes and vacuoles.
13. List the steps for the proper set-up, use, and take down of an optical microscope.
14. Describe how modern science and medicine depend on the invention and development of the
microscope.
15. A wave representing visible light is shown below. Identify the wave, i or ii, that would most likely
represent an electron wave.
i.
Select the magnification (start at low magnification) that works best to view your specimen. Include:
• a diagram or photograph of what is seen through the microscope.
• the identification of specimen.
• the magnification information (show work)
Place one drop of pond water on a slide and add cover slip.
View under low, then medium, Include
• a diagram or photograph of what is seen through the microscope.
• the identification of specimen.
• the magnification information.
Note:
• Always start with the low power objective lens.
• Only use the coarse adjustment dial with the low magnification.
• Do not use the coarse adjust dial when using high magnification.
• Use different light intensities to better view your samples.
• Place diagrams inside neat circles. Always use a ruler when drawing straight lines.
Place one strand of your hair on a slide and add a cover slip
View under low, then medium, then high. Include:
• a diagram or photograph of what is seen through the microscope.
• the identification of specimen.
• the width of a single strand of your hair.
3. Cells come from previous cells according to the cell theory. But if the reproduction of cells occurs in
an uncontrolled manner the entire organism may be endangered. Cells that reproduce in this way are
called cancer cells. Investigate further and write a summary about cancer at a cellular level.
5. Vacuoles and lysosomes are vesicles found in cells. A vacuole and a lysosome have been compared
to bringing groceries into a house in a bag (vacuole), and taking the garbage out in a bag (lysosome).
Comment on the validity of the analogy.