VMC-221: Veterinary Immunology and Serology (1+1)

Question Bank
Objective type Questions

Q. No. 1 - Fill up the blanks with correct words

1. The British physician, who developed the first

vaccine against an infectious disease is
2. The two effector molecules of specific immune
response are
&
3. The cells that respond first to an antigen is
4. The antibodies that are attached to the surface of
B cells are referred as
5. The differentiation markers of T lymphocytes are
&
6. Extracellular phagocytosis is performed against
parasites by
7. The high affinity binding CD receptor for Fc portion
of antibody is
8. The small antigens that cannot stimulate the
production of antibodies are
9. The antibody isotype that is more prevalent in
anamnestic response is
10. The TLR that binds with the LPS of bacteria is
11. The protein of MHC II, that prevents the binding of
other protein at the cleft region at rough ER is
12. The TCR-CD3-zeta chain complex is responsible for
(function)
13. The communication molecules of immune system
are
14. The receptor that strengthens the bond between
TCR and MHC II is
15. The effector in delayed type hypersensitivity of cell
mediated immune response is
16. The lymphocytes that mediate ADCC is
17. The classical complement pathway starts with the
activation of
18. The immediate allergy with systemic response is
Q. No. 2 - State whether the statements are true of false, if false, correct them.

No. Statement
The development of granuloma in Type IV hypersensitivity is due
TRUE /
1
to the inability of the pre CTL to convert into active CTL FALSE

2 Injection of allogenic immunoglobulins are responsible for serum TRUE /

sickness (Type III hypersensitivity) in animals FALSE

3 The alternate complement pathway contributes to the specific TRUE /

immune response FALSE

4 Interaction between Th1 cell and B cell results in the proliferation TRUE /
and differentiation of B cell and production of antibodies FALSE

5 The non-specific killing of cells having endogenous antigen is TRUE /

mediated through macrophages FALSE

6 The only mechanisms by which activated CTL performs lethal hit TRUE /
is through osmolysis FALSE

7 High affinity IgM and IgG are produced by plasma cells activated TRUE /
by carbohydrate antigens FALSE

8 The term epitope is associated with both T dependant and T TRUE /

independent antigens FALSE

9 Specific immunity is produced as a result of interaction of T cells TRUE /

and B cells alone and no other cell is involved FALSE

10 Professional antigen presenting cells are not associated with the TRUE /
processing of endogenous antigens FALSE

11 Only epitopes presented by MHC alone can stimulate Abs or CTL TRUE /
FALSE
12 The hypohalide compounds formed inside the phagocytic cells is TRUE /
responsible for the destruction of foreign substances FALSE

13 The terms, Pattern recognition receptors (PRR) – Pathogen TRUE /

associated molecular patterns (PAMP) are associated with FALSE
scavenging receptors
 14 GALT, MALT, BALT and NALT are organized primary lymphoid TRUE /
tissues FALSE

15 The binding of CD40 of APC with CD40L of helper T cell facilitates TRUE /
conversion of pre CTL to activated CTL FALSE

16 Macrophages have a single function of doing multiple TRUE /

phagocytosis and remain as matured cells in the blood circulation FALSE

17 Immunoglobulin family comprises of different isotypes and TRUE /

allotypes of antibodies alone. FALSE

18 The flexibility of certain isotypes of antibodies is due to the TRUE /

interaction of framework region in variable and constant domains FALSE

19 IgA exist as monomer in local secretion and dimer in serum TRUE /

FALSE

20 Opsonization is an efficient process of phagocytosis TRUE /

FALSE

Q. No. 3-1 - Match the following

1 Louis Pasteur A Complement 1

2 Memory cells B Dendritic cells 2
3 Th1 cells C IgE 3
4 Th2 cells D ADCC 4
5 Macrophages E Birbeck granules 5
6 Dendritic cells F Garbage collectors 6
7 NK cells G B cell activation 7
8 Mast cells H Inflammation 8
9 Professional APC J bcl 2 9
10 Jules Bordet K Rabies vaccine 10

Q. No. 3 -2 - Match the following

1 Signal transduction A Lytic pathway 1

2 Antibody B Long hinge region 2
3 C3b C Type II hypersensitivity 3
4 Helper T cells D Fas receptor 4
5 Cytotoxic T cells E IL 2 5
6 MAC F CD8 6
7 Apoptosis G CD4 7
8 Autoimmunity H CD35 8
9 IgD J CD64 9
10 Cytokine K CD3 10

Q. No. 4 - Expand the following

1 Ab

2 Ag

3 MAC

4 Ig

5 TLR

6 ADCC

7 APC

8 CTL

9 CD

10 GALT

11 TCR

12 BCR

13 BoLA

14 IL

15 CLIP

16 PMNL

17 CDR

18 Fc and Fab

19 ELISA
20 MALT

Subjective type Questions

Q. No. 1 - Differentiate / Explain the following

1. CDR and Frame work regions

2. What is the basis for allergic reactions?
3. What will happen when the complement system fails to get activated?
4. Why there is a T cell repertoire and why a single T cell is having 10,000-20,000 TCR?
5. Why substances like nylon, metal pins and flagellin are poorly antigenic?
6. What is the principle behind cross reaction involving Forssman antigen and
heterophile antibodies?
7. Why there is strong inflammatory response during tuberculin testing in animals
already exposed to tuberculosis?
8. What is the advantage of Fc portion of antibodies having same amino acids
irrespective of differences at the Fab?
9. Why every time a bacterial carbohydrate antigen is treated as naïve antigen unlike
protein antigen?
10. How TLRs contribute in innate immune response?
11. What is the disadvantage in giving xenogenic immunoglobulins against an acute
disease?
12. What are the two primary roles of the thymus?
13. What effect would removal of the bursa of Fabricius (bursectomy) have on
chickens?
14. Name the different types of immunoglobulins
15. Name the antibody that is involved in allergy.
16. Name different types of mucosa associated lymphoid tissue
17. Name different pathways of complement system.
18. What is the function of memory cells?
19. Which is largest secondary lymphoid organ?
20. Where are peyer’s patches located?
Q. No. 2 - Write briefly on the following
1. Differentiate the methods of exogenous and endogenous antigen processing.
2. Draw a flow chart of sequence of action during respiratory burst?
3. List the products of C3, C4 and C5 and their function
4. Memory cells
5. List the three mechanisms of CMI and briefly explain ADCC
6. Name three features of a secondary immune response that distinguish it from a
primary immune response
7. Name the primary and secondary lymphoid organs.
8. Describe the functions of various secondary lymphoid organs.
9. Compare antigen with immunogen.
10. Differentiate between TCR and BCR.
11. What are monoclonal antibodies?
12. Write down the functions of complement.
13. Name three major events of inflammation
14. Differentiate between systemic and localized infection
15. Differentiate between agglutination and precipitation.
16. Differentiate between primary follicle and secondary follicle
17. Differentiate between red pulp and white pulp.
18. Differentiate between stem cell and haematopoitic stem cell
19. What are three subpopulations of lymphocytes?
20. Write two characteristic features of mature B-lymphocytes

Q.No. 3 - Write short answers on the following

1. Explain the sequence of reactions as a result of complement activation by free

antigens with a neat flow diagram (or) sequence of reactions as a result of
complement activation by antigen-antibody complex with a neat flow diagram.
2. Role of physical barriers in innate immune response.
3. Cytokines
4. What is immunity? How it differs from immune response?
5. Differentiate between lysozome and phagosome.
 6. Differentiate between innate and acquired immunity.
7. What are the major types of cells in addition to T- lymphocytes which make up the
frame work of the thymus?
8. Where the lymph nodes are located and what function they perform?
9. What are the reasons for the occurrence of autoimmune disorders?
10. Write a short note on Antigen presenting cells.
11. Role of sectretory immunoglobulins
12. Assessment of immunity and protection status of birds against Newcastle disease
13. Difference between live and inactivated veterinary vaccines
14. Difference between Anaphylaxix and Prophylaxix
15. Define primary and secondary binding immunoassays with examples
16. Difference between vaccination and variolation

Q. No. 4 - Write long answers

1. (a) Differentiate B and T lymphocytes based on their morphology, functions,

receptors and products
(b) Differentiate the mechanism of phagocytosis between neutrophils, eosinophils
and macrophages
(c) How mast cells de-granulate and what are its impacts?

2. (a) Differentiate the structural and functional features of isotypes of antibodies

(b) Differentiate innate and specific immune responses based on cells involved,
products and mechanisms

3. (a) How antibodies are produced against protein and non-protein antigens?
(b) List the different theories of antibody production and explain clonal proliferation
theory
4. (a) Write in detail the different classifications of antigen
(b) Explain the primary structure of antibody
5. What is hybridoma technology? Write different steps of monoclonal antibody
production and application of monoclonal antibody in animal health.
6. Enumerate antigen antibody reactions. Describe the principle methodology and
 clinical applications of agglutination reactions.
7. What is meant by immunity and explain about the various components involved in
the immune system.
8. Define antibody and describe about their structure in detail with a neat diagram?

9. Explain the principle and applications of Radial and double immunodiffusion

10. Discuss the various components of Innate immune response.

11. Classify and define hypersensitivity. Describe type I I hypersensitivity.