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  • StreakPlateStepsCredits 2

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    Rahmasari Nur Setyono
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    The document describes the steps to perform a streak plate technique. The steps are: 1) sterilize an inoculating loop by heating it in a Bunsen burner flame, 2) spread an isolated bacterial colony over 1/4 of an agar plate using parallel streaks, 3) flame the loop and streak the bacteria into the next quadrant at a 90 degree angle without overlapping, 4) repeat flaming and streaking the bacteria into the remaining quadrants, and 5) incubate the streaked plate upside down at 37°C for 24 hours.

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    StreakPlateStepsCredits-2

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    The document describes the steps to perform a streak plate technique. The steps are: 1) sterilize an inoculating loop by heating it in a Bunsen burner flame, 2) spread an isolated bacterial colony over 1/4 of an agar plate using parallel streaks, 3) flame the loop and streak the bacteria into the next quadrant at a 90 degree angle without overlapping, 4) repeat flaming and streaking the bacteria into the remaining quadrants, and 5) incubate the streaked plate upside down at 37°C for 24 hours.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    6 views1 page

    StreakPlateStepsCredits 2

    Uploaded by

    Rahmasari Nur Setyono
    The document describes the steps to perform a streak plate technique. The steps are: 1) sterilize an inoculating loop by heating it in a Bunsen burner flame, 2) spread an isolated bacterial colony over 1/4 of an agar plate using parallel streaks, 3) flame the loop and streak the bacteria into the next quadrant at a 90 degree angle without overlapping, 4) repeat flaming and streaking the bacteria into the remaining quadrants, and 5) incubate the streaked plate upside down at 37°C for 24 hours.

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    © All Rights Reserved
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    Streak Plate technique

    Steps - click “Start”

    1. Sterilize the inoculating loop in the Bunsen burner by clicking on the loop and
    dragging it to the burner. Put the loop into the flame until it is red hot. Allow it
    to cool.
    2. Pick an isolated colony from the agar plate culture and spread it over the first
    quadrant (approximately 1/4 of the plate) using close parallel streaks.
    3. Flame the loop.
    4. Turn the plate 90 and lightly sweep the loop 1-2 times through the inoculated
    area, then streak into the next quadrant without overlapping the previous
    streaks.
    5. Flame the loop.
    6. Turn the plate 90, overlap the previous area 1-2 times, and streak into the
    next quadrant (step 4).
    7. Flame the loop.
    8. Repeat #6, streaking the remainder of the plate.
    9. Invert the plate and incubate at 37C for 24 hr.

    Credits

    Designed by Virtual University Design and Technology at Michigan State


    University
    Faculty: Cindy Arvidson, PhD
    Producer: Jiatyan Chen

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