Pharmacology practical

(Effects of drugs on the ciliary motility of forg oesophagus)

By : Osama Al-Zahrani
@OSAMA_Z96
Introduction :
- Cholinergic drugs causes contraction of cilia leading to increased movements.
- Anticholinergics drugs causes paralysis of cilia leading to decreased movements.

Objective :
- To find out the action of certain drugs on the ciliary motility of frog oesophagus.

Materials :
1- Frog.
2- Poppy seeds.
3- Frog wooden board.
4- Stop watch
5- Drugs & Solution :
A) Acetylcholine 10% : Cholinergic agonist.
B) Physostigmine 10% : Anti-choline esterase inhibitor.
C) Atropine 0.1% : Non-selective muscarinic agonist, or Cholinergic antagonist.
D) Frog Ringer : Nutrient solution To keep the tissue viable.
 Materials pictures

Frog wooden board Poppy seeds

In Situ experiment
 Experiment results

Serial Drug Reading 1 Reading 2 Reading 3 Mean

1 Ringer (Control) 34 s 29 s 33 s 32 s

2 Acetylcholine 22 s 18 s 17 s 19 s

3 Physostigmine 14 s 14 s 14 s 14 s

4 Atropine 43 s 46 s 51 s 46 s
 Experiment results
50

45

40

35

30

25

20

15

10

0
Ringer (Control) Acetylcholine Physostigmine Atropine
 Interpretation of the results
- The most effective drug is Physostigmine,
because the drug take short time to increase the movement.

- The order of the drugs depending on the shortest time as following :

1- Physostigmine
2- Acetylcholine
3- Atropine