COMPARING THE ANTIOXIDANT ACTIVITY AND

TOXICITY LEVEL OF Eleutherine bulbosa (Mill.) Urb,

Solanum betaceum AND Synsepalum dulcificum
EXTRACTS
Casey Vira Aprillea, Muhammad Akbar Ramadhana Nasution,
Rara Poetri Claudya, Eny Yulianti, David Limanan, Frans Ferdinal
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tarumanagara University, Jakarta, Indonesia
*Corresponding Author : caseyvir@gmail.com
th
THE SIXTH GRUBER-SOEDIOGO LECTURE & 24 National Seminar of Indonesia Society for
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Biochemistry and Molecular Biology, Bandung, November 5 -7 2019

BACKGROUND OBJECTIVE
Indonesia is endowed with rich natural resources. To investigate the antioxidant activity and
The fertile soils of Indonesia support varieties of toxicity level of methanolic extract
: Solanum betaceum, Eleutherine bulbosa
plant's growth. Some of plant's that were used for
traditional medicine such as Dayak Onion's bulbs (Mill.) Urb and Synsepalum dulcicum.
(Eleutherine bulbosa (Mill.) Urb),Tamarillo's fruits
(Solanum betaceum), and Miracle Berry's leaves
(Synsepalum dulcicum).
METHODS
Those plants contain many benets such as
antioxidant that can suppress Reactive Oxygen Researchers use DPPH to detect free-
Species (ROS). Beside their antioxidant role, they scavenging activity and BSLT to measure
also have anti-cancer, anti-inammation the toxicity level of each methanolic extract,
properties and many more. using Artemia salina Leach.

RESULTS
DPPH scavenging assay showed that the methanol extract of Eleutherine bulbosa (Mill.) Urb
have an IC50 value of 109.369 μg/mL Solanum betaceum have an IC50 value of 794.318 μg/mL,
and Synsepalum dulcicum have an IC50 value of 200.750 μg/mL.
inhibisi (%)
inhibisi (%)

inhibisi (%)

konsentrasi (μg/mL) konsentrasi (μg/mL) konsentrasi (μg/mL)

BSLT showed that the methanol extract of Eleutherine bulbosa (Mill.) Urb have a LC50 value of 65.690 μg/mL,
Solanum betaceum have a LC50 value of 140.026 μg/mL, and Synsepalum dulcicum
have a LC50 value of 153.735 μg/mL.

BSLT - Eleutherine bulbosa

120

100
mortalitas (%)

mortalitas (%)
mortalitas (%)

80

60

40

20

0
0 0,5 1 1,5 2 2,5 3 3,5

konsentrasi (μg/mL) konsentrasi (μg/mL) konsentrasi (μg/mL)

CONCLUSIONS 1. Halliwell B, Gutteridge JC. The denition

and measurement of antioxidants in
This study suggests that the extract of Solanum betaceum, Synsepalum biological systems. Free radic Biol Med
dulcicum, and Eleutherine bulbosa (Mill.) Urb exhibit great potential 1995; 18: 124-6.

for antioxidant and anti-cancer. 2. Jat, Deepali & Nahar, Manisha. (2017).
The result of this research showed Oxidative stress and antioxidants: an
overview. IJARR. 2. 110-119.
that Eleutherine bulbosa (Mill.) Urb
has the highest antioxidant and 3. Bondet V, Brand-Williams W, Berset C.
anti-cancer contents among Kinetics and mechanisms of antioxidant
activity using the DPPH free radical method.
the others. Lebensmitt Wissenschaft Technologie Food
Sci Technol. 1997;30:609-615.
doi: 10.1006/fstl.1997.0240.