Becmwus HBI'Y. No 1/2015 MATEMATHYECKHE M ECTECTBEHHBIE HAVKH ‘The researcher has obtained chitosan from three kinds of raw materials with such purity and physico-chemical characteristics which allow us to use these materials not only for industrial purposes, but also as dietary supple- ‘ments. ‘The author has used potentiometric titration to determine the degree of chitosan deacetylation, The obtained chitosan samples were characterized using IR-spectroscopy. The study of the spectra of obtained chitin samples has confirmed the equivalence of the resulting chitosan to reference samples. Key words: dietary supplements; chitin; chitosan; Infrared spectrometry; potentiometric titration About the author: Svetlana Yurievna Soldatova, Candidate of Engineering, Assistant Professor at the Depart- ‘ment of Biotechnology and Technology of Bioorganic Synthesis, Place of work: Moscow State University of Food Production, ‘YAK $77,114,083 M.B.Bonoaxooa CH.Bymoca G.10.Condamoca Mockea, Pocus MOJAYYEHHE HM HCNOIb30BAHHE IKCTPAKTA KPACALLHX BEUJECTB BAHOFPAIA B KOCMETHYECKHX MPOAYKTAX Anmoranust, B crarse onyOnnKonann peaynsrars HecnexORaNnT, NOCAAMENABIX 9KeTPaKIUIH GHOTOFNECKHL AKTHBHEAX pentecTB H9 Aro BHHorpazta Vitis Vinifera copra HoaGenna, w nepayio O%epenb, (yraoH.oHtA0B. Hay'ert Guoxiivecknii cocras HexLNEX FOX SUMOTpALta, a TAKE HX OTAeDNX MaCTeM (KOKHMA, MAKOTL, KOCTOUKA). Tloxasawo, #10 wanGomuee KoANYeCTHO dpasIaKOHOHLIOK COnePAUTER 8 BIMOTPaRNOI MOCTONKE, KPACHIIHIX e- eer — 8 KORINE. 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GuoxiMMeckHe noKasaTem SAMTEPATYPA 1. Avepisnowa E.B., Lkomuukona M.H., Eroposa E10, buswaroruseckit axrumunie neulectsa pacrurensoro coupes. Bitiex, 2010, 2. Bototos B.M. Pacumpenie ravouet akert1yaraumonix exolicrs npupoaHbIX KpacTeselt ns pactiTeaBHoro cuiphs // Xpanenute n nlepepaGorka cenbxosciipbs. 1999, Ne 6, 3. Batoros BM., Casuun [LH. Sdypextunuocts mpouecca 9xctpakuut a¥TouaHouBX uFMeHTOR pH pas- ansix yetositax opaGorks pacritestsHoro cuipsst i/ Becr. BETA. 2009, Ne L 4. Konaparses JLB., Ierios H.F. CnocoSsi noxyerna 2xctpakra BIMOTpAIHBIX BEIAUNOK H BOSMOAHOCTHL ero Henloabaouauuia » auutenoili npombiuzeniocTa // Hanecrua nyzon, Thintenaa rexuoxorus, 2009. Xe | 5. Timur A.B., Myxrapos 9.H., Myxtaposa C., Kanutyu A.I1. Oaanouons xpacuoro suorpana Vitis Vinife- — nepeniexrussi npumenenun w MexuMHe H KoeMeTHKe // Kocwenaka # meauutHa, 2005. 6. Purkoua HB, Hsawona JLA., Mypaeueo E.H. Coneputecruonanie cocoGos 2xcrpaxi(uit xpacailurx wemtects na pacrirrenbutoro cuapos // Xpantenne u nepepaorka cespxoactapea. 2006, Ne 5. REFERENCES, 1. Averyanova E.V., Shkolnikova M.N., Egorova E.Yu. Fiziologicheski aktivnye veschestva rastitelnogo syrya [Physiologically active substances of plant material). Biysk, 2010 (in Russian), 2. Bolotov V.M. Rasshirenie gammy ekspluatatsionnykh svoystv prirodnykh krasiteley iz rastitelnogo syrya [Ex- panding the range of performance properties of natural pigments extracted from plants) // Khranenie i pererabotka selkhozsyrya, 1999, Ne 6 (in Russian. 3. Bolotov V.M., Savvin PN, Effektivnost protsessa ckstraktsii antotsianovykh pigmentov pri razlichnykh uslo- viyakh obrabotki rastitelnogo syrya [Efficiency of extracting anthocyanin pigments in various conditions of pro- cessing vegetable raw materials} // Vestnik VGTA [Proceedings of Voronezh State Technological Academy], 2009. Ne 1 (in Russian), 4, Kondratev D.V., Scheglov N.G. 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No 1/2015 MATEMATHYECKHE M ECTECTBEHHBIE HAVKH M.V.Bondakova S.N,Butova S.¥uSoldatova Moscow; Russia COLLECTING AND USING GRAPE EXTRACT AS A COLORANT IN COSMETIC PRODUCTS, Abstract, The paper presents the results of studies on the extraction of biologically active substances, the flavo- noids primarily, from grapes Vitis Vinifera Isabella. The rescarchers have studied the biochemical composition of whole grapes, as well as their parts (peel, pulp, bone) and have shown that the most amount of flavonoids are found in grape seeds, while most coloring agents are contained in the pel “To be competitive, modern cosmetic products should provide a quick visible effect, be attractive, but also contain preferably natural biologically active substances, which stimulate metabolism and reparative functions of the human, skin, The increasing interest of cosmetic producers and consumers to the natural ingredients is associated with both strict limitation in terms of synthetic compounds, and the producers” desire to make their produet more “natural”. The research shows that the demand for “green cosmetic Europe has been constantly growing over the past decade, “Most popular cosmetic products are those which contain antioxidants helping to protect skin cells from oxidative stress, the negative effects of UV radiation, and thus protecting the skin against premature aging. The natural antiox- idants include bioflavonoids, which are natural colorants. This is a group of phenolic compounds, one of the most common and numerous classes of biologically active substances containing aromatic rings with free or bound hy- droxyl group. The phenolic compounds, with the aromatic ring contained more than one hydroxyl group, are called polyphenols. The researchers have determined the optimal parameters for the extraction process. Specifically, to extract flavo- noids from grape peel i s required to use 99% ethanol as an extractant, and produce the extraction atthe tempera- ture of 60°C, with pH $.0—6.0 liquor ratio of 1:2 for 30 minutes. To increase the yield of biologically active sub- stances its recommended to freeze the material at a temperature of -18°C. The authors have studied the composition of the obtained extracts, including the coloring substances and phenol- ‘ic compounds in equivalent of gallic acid and determined the technological characteristics of the obtained extracts, such as resistance to light, storage duration, and temperature. The study has shown that the resulting colorant retains its consumer properties after 4 weeks at a temperature no higher than $°C when kept in @ dark place. The optimum PH should be from slightly acidic to neutral. When kept under the light conditions, the color remains stable for 3 days. The studies help optimize the technology of producing grape extract in order to use it as a colorant and source of biologically active substances in the manuficture of cosmetic products The authors have developed the formulation of cosmetic lotions with different concentrations of extracts and studied their organoleptic and physico-chemical characteristics, The study has showed that al lotions samples corre- spond to normative documents in terms of their physical and chemical properties Key words: flavonoids, extraction; grape extract; biologically active substances; natural colorants; consmetic products About the authors: Marina Valerievna Bondakova', Candidate of Engineering, Deputy Chief of the Laboratory: Svetlana Nickolaevna Butova', Doctor of Biology, Professor, Head of the Department of Biotechnology and Tech nology of Bioorganic Synthesis; Svetlana Yurievna Soldatova’, Candidate of Engineering, Assistant Professor at the Depariment of Biotechnology and Technology of Bioorganic Synthesis, Place of work: ' LLC AB Tower; *’ Moscow State University of Food Production,