Exercise # 3

Nucleic Acid Amplification

(Decision making and Loop)
Date : March 09, 2021

Aim
To calculate G/C content, or ratio on DNA molecule.

Description

The G/C content, or ratio, is the percentage of nitrogenous bases on a DNA molecule
which are either guanine or cytosine. In PCR procedures, the GC content of primers are used
to determine their annealing temperature to template DNA. A high GC content level indicates
a higher melting temperature as it takes more energy to break three hydrogen bonds versus
two. Here is the formula:

Here is a sample calculation. You have a fragment of DNA with the following structure:

AAGGTATCGGTATCGAAATT

Count up the bases and plug into the formula: 7 As, 6 Ts, 5 Gs and 2 Cs

(5+2)/(7+6+5+2) * 100 = 
7/20 * 100 =
35% G/C Content

Determine the G/C content of the following 3 samples and answer question #4.

1. AATTTGGGCCCATTACATAC
2. CCGAGGAAGATGTAGGCCG
3. ATAGTTCATAGTCTATGACG
4. Which of the 3 given examples of above will, theoretically, have the highest
Temperature of Melting?

Instructions:
Use foreach, elsif (if...elsif...else...)

Ref:
https://www.perl.com/article/perl-foreach-loops/ 
https://www.perltutorial.org/perl-if/ 
https://gist.github.com/ishu3101/00c296fcb04bb10f4c2d5fbff894b06a