‫اﻟﻤﻌﻤﻞ اﻻول‬

‫ﻣﯿﻜﺮو‬

Urine culture
Yaser Musawa

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Aim of the test :

 An etiological diagnosis of bacterial urinary tract

infection by semi quantitative cultivation of the
urine with identification and susceptibility test of
the isolated bacteria(s).

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Types of specimen:

 Urine.
 Suprapubic aspiration.
 Catheterized urine.
 Diaper collection, and sterile bag.
 Clean Catch.
 MSU.

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Criteria of specimen rejection:

 Un-refrigerated specimen older than 2 hours.

 Unlabeled or mislabeled specimen.
 24 hours urine specimens?????????????
 Bedpan urine??????????
 Bag urine???????????

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Pre specimen processing:
Patient preparing:
 Instruct the procedures for the patient Specimen
collection
 Patient not needing assistance:
Give the patient a suitable container.
Instruct the patient before the collection, preferably with
illustration.
Tell the patient not to touch the inside or rim of the
container.

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Pre specimen processing:

 Who will collect the specimen:

 Midstream urine is collected by the patient.
 If disabled, nursing staff will assist in collection.
 For catheterized specimen, nursing staff will collect the specimen.
 Suprapubic aspiration is performed by the physician.

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Pre specimen processing:

 Quantity of specimen
 To fill line in transport tube (~20 mL).
 Time relapse before processing the sample
 The maximum time allowed for processing a urine sample is 2 hours from the
time of collection.
 Storage
 At room temperature unless delay is inevitable; it must be refrigerate or mixed
with preservative like boric acid.

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Pathogens:

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Specimen processing:
PUS CELLS,
BACTERIA
MICROSCOPY

GRAM STAIN

UTI
QUANTITATIVE
METHODS

SEMIQUANTITAVE
CULTURE
METHOD

ANTIBIOTIC
SENSITIVITY
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 LABORATORY EXAMINATION OF URINE
DAY-1
 Describe the appearance of urine.
 MICROSCOPIC EXAMINATION:
 Wet preparation : To detect:
 Significant pyuria,(WBCs in excess of 10 cells/µl of
urine.counting -1WBCs per low power fieldcorrespond to 3 cells
per/µl ).
 Red casts
 Yeast cells
 T.vaginalis motile trophozoites
 S.haematobium eggs
 Bacteria(provided urine is freshly collected).

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 LABORATORY EXAMINATION OF URINE
DAY-1
 GRAM STAINING:
The gram stain is the easiest, least expensive, and
probably the most sensitive and reliable screening
method.
Presence of at least one organism per oil immersion
field.( examining 20 fields ) correlates with
significant bacteria in urine (>10^5 CFU/ml).

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DAY-1
Culturing:
 Mix the urine sample to re-suspend microorganism present.
 Dip a 1 μl or 10 μl calibrated loop in vertical position in the
urine and remove the loop and use the collected fluid to
inoculate Nutrient, Blood, CLED and MacConkey agars
respectively

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13
 DAY-2
EXAMINE AND REPORT THE CULTURE:
 0.01 ml inoculum:
 No. of colony * 100 CFU/ml.
 0.001 ml inoculum:
 No. of colony * 1000 CFU/ml.

 No significant growth:
 <10,000 organisms/ml, not significant.
 10,000-100,000,doubtful significance.
 Positive cultures:
 >100,000/ml, significant bacteria.

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