W O R K S H E E T

Activity No. 7
Name/s: Jingco
Morones
Magnaye
Daham
Dichuasido
Group no.: 1
Result and Observation:

Observations after 48 hours

Table 1. Colonial characteristics of microorganisms using different methods

of isolation
Streak Plate Method Spread
Pour Plate Plate
in petri dish in test tube Method Method
Moderate Abundant Scanty Scanty
Amount of
Growth

Circle echinulate Circle Circle

Form

Flat effused Flat Flat

Elevation

Entire undulate Entire Entire

Margin

IV. Questions:

1. Compare the pour plate and streak plate methods of isolating bacteria?
The main difference between streak plate and pour plate is that in streak plate, the
first to be added is the melted nutrient agar and the second to be added is a loop of
bacteria from a slant, whereas the first to be added in pour plate is the bacterial broth
and the second to be added is the nutrient agar. Furthermore, the volume of inoculum
in the streak plate is only a loopful from a bacterial slant while the volume of inoculum
in pour plate is 1.0 to 0.1 mL. Moreover, streak plate is for the isolation of colonies
while the pour plate is for counting the number of colonies.
2. In which plate do you find the surface and the deep or buried colonies?
Although the spreading plate is used to isolate bacterial colonies only on a medium
surface, and the spreading plate is used through a sterile inoculating loop (or needle)
an inoculum scattered over the surface of a solid medium in the plates of Petri is
found, only on the surface of or on the surface of the medium are colonies on the two
string plates and the spreading plate.

3. How do surface colonies differ from deep or buried colonies?

the difference is between the two is their oxygen tolerant and aerobic bacteria growing
on top of the surface whilst the anaerobic bacteria colonizes the deeper areas.

4. Which is rapid in proving the purity of a culture – on an agar or in a broth?

Agar. To prove purity, you must have one type of colony, and have some single
colonies on an agar plate. Colonial morphology (size, shape, color etc) must be the
same for all the colonies. Broth will grow bacteria very quickly, but you can't discern
purity by looking at a wet mount.

5. Why are petri dishes incubated in an inverted position?

Petri dishes need to be incubated upside-down to lessen contamination risks from
airborne particles landing on them and to prevent the accumulation of water
condensation that could disturb or compromise a culture.

Also, it is kept in an inverted position (upside down) during incubation so that

condensation droplets will not fall on the agar's surface (as such droplets are potential
sources of contamination).

Aside from that, the evaporation of water from the media if incubated in the normal
position could cause the media to start drying thereby affecting the ideal microbial
growth conditions and increasing microbial count errors.
VI. Conclusion
In conclusion, in obtaining a pure culture we mus transfer a small sample into new,
sterile growth medium in such a manner as to disperse the individual cells across the
medium surface or by thinning the sample manyfold before inoculating the new
medium. The importance of pure culture in field of microbiology it helped link the
causal nature of microbes to certain diseases, such as anthrax. As developed by
Koch, pure cultures allow the pure isolation of a microbe, which is vital in
understanding how an individual microbe may contribute to a disease. Lastly,
distinguishing the features of bacterial growth might take use of these steps (1) get
pure culture of your bacteria for each type (2-3 stable strains);
(2) find basic morphologic and biochemical traits (as Mesgor advised), FAME, and
establish the group of bacteria.
(3) if you have well-nown species, use specific PCR primers developed for them;
(4) if if you have new bacteria - use consensus 16S rRNA prmers to get at least 400
bp fragment and sequence it - you will find the genus (at least).
(5) use MLST for this particular genus to find if it belong to known species or describe
new one.