Worksheet 2.

2 Detecting respiration
in yeast
Yeast is a microscopic, single-celled fungus. Like all living organisms, yeast respires.
Living organisms break down glucose and produce carbon dioxide when they respire.
We can detect carbon dioxide using limewater. Limewater is a clear, colourless liquid. When
carbon dioxide is present, the limewater goes milky white.
Set up your apparatus like this. Leave it in a warm place.

yeast
suspension bubbles of
limewater carbon
and glucose
dioxide gas

Questions
1 Explain why glucose is added to the yeast in the tube.

To produce carbon dioxide.

2 Explain why there is a rubber bung in the tube containing the yeast.
To control the tube movement .

3 What happened to the limewater? Explain why this happened.

When we added corbon dioxide into tube the it's color get milkywhite.

Copyright Cambridge University Press 2012 Cambridge Checkpoint Science 7 1

 Worksheet 2.2

4 Think about how you could use this apparatus to test the idea that yeast respires more quickly
when it is warm than when it is cold.
a What variable would you change in your experiment?

I would change temperature .

b How could you change this variable?

To change the place.

c What variables would you keep the same?

Yeast .

d How could you measure how quickly the yeast is respiring?

Place a rubber bung with a delivery tube into the neck of the round bottomed flask and plac

Copyright Cambridge University Press 2012 Cambridge Checkpoint Science 7 2