Figure S1

350
group name nr. complementary sequences
300

CACGTG 356 cacgtg; gtgcac

CATGTG 286 catgtg; gtacac; cacatg; gtgtac
250

CACCTG 57 cacctg; gtggac; caggtg; gtccac

CAAGTG 53 caagtg; gttcac; cacttg; gtgaac
150 200
number

CAAATG 44 caaatg; gtttac; catttg; gtaaac

CAACTG 37 caactg; gttgac; cagttg; gtcaac
CATATG 28 catatg; gtatac
100

CAGATG 19 cagatg; gtctac; catctg; gtagac

CAGCTG 18 cagctg; gtcgac
50

CAATTG 16 caattg; gttaac

0

C TG

C TG
C TG

C TG

AG G
C TG

C TG
C TG

C TG

G
AT

TT
G

C
C

G
G

C
A

A
AG

AA
AA
AC

AT
AA
AC

AA
AT
C

C
 A.
0.25 PIL1 HFR1 PIN3 IAA19

0.2

0.15
Input (%)

0.1

0.05

0
Col PIF4-HA PIF5-HA Col PIF4-HA PIF5-HA Col PIF4-HA PIF5-HA Col PIF4-HA PIF5-HA

B. C.
GAI 0.1 XTR7
0.1
Input (%)

Input (%)

0.05
0.05

0
0
PIF5-HA
PIF5-HA

Chromatin Immunoprecipitation (ChIP) of PIF5-HA after 2 hours in low R/FR. Immunopre-

cipitated DNA was quantified by Q-PCR using primers in the promoter region containing a
G-box or control region (minimum 1 kb 3’ or 5’ from the peak region). Data are average of
technical triplicates of the Q-PCR.

(A / B) Col and/or PIF5-HA lines were grown for 7 days in constant light conditions before
being shifted for 2 hours in low R/FR conditions.

(C) PIF5-HA lines were grown for 14 days in constant light conditions before being shifted
for 2 hours in low R/FR conditions.