Osmosis Experiment

Abstract

Keywords: Osmosis,

Introduction
 Osmosis is the movement of solvent molecules such as water from a low solute
concentrated solution (high water potential) to a higher region of solute concentration (low water
potential) through a semi-permeable membrane. There are three types of solution that will be
explored throughout this experiment.
1. Hypotonic Solution - a solution in which the solution and the cell do not have the same
concentration of solute and water. A hypotonic solution contains more solutes inside of
the cell than outside which makes water flow inside of the cell causing it to swell and
often burst due to pressure (Deplasmolysis).
.
2. Isotonic Solution – a solution where all the concentration is in equilibrium. In an isotonic
solution, the concentration of solute and water inside and outside of the cell are equal
which means there is no net movement of solute and solvent.

3. Hypertonic Solution – a solution that has a higher solute concentration outside of the cell
than inside. This will cause water inside of the cell to flow out of the cell making it shrink
(Plasmolysis).

It is important to understand the concept of osmosis, things such as injecting fluids in the
body, one must carefully understand what type of solution must be made because it affects how
the cell intake what’s in that solution.

The objective of this experiment is to:

1. Determine what type of osmotic solution of each solute concentration
2.

Related Literature
In an online article, a close and similar study was conducted and posted by Yas Asghari
(2017). The study tackled about the effects of osmosis on potatoes in different concentrations of
sucrose solutions. In the result of the experiment, the hypothesis about whether the potato
chores should increase in size while submerged in different sucrose concentration, was correct.
The potato chores that are submerged in distilled water and in the sucrose solution 0.2 mol/dm3
were turgid in texture while in increasing mol/dm3, the texture of the potato chores were quite
flabby and squishy.

Materials and Methods

 To test our hypothesis, we used a method similar to the famous potato experiment but a
different root vegetable such as carrots. The carrots were carefully peeled and made strips in
rod shapes then measured by a ruler to get all strips in the same length (10 cm) as accurate as
possible. Distilled water is used as a solvent since it contains less impurities which means it has
a high water potential better than other types of water. Four glass cups filled with distilled water
will be used in this experiment. The volume of water is then measure by a flask with labels, each
glass must contain 100 ml.

Study Area
This study was conducted inside our home in Butuan City, Agusan del Norte. Due to the
pandemic, this is the only facility that is only applicable for this study.

Collection of Specimen
All of the carrots that are used in this experiment are freshly taken out in Ragas Farm
located in Cabadbaran City in the province of Agusan del Norte. The carrots were cleaned using
only distilled water and preserved inside a refrigerator to prevent from rotting to fast.

Results and Discussion

Salt Concentration Initial Length Final Length Change in Average Change

g/100ml (cm) (cm) Length in Length
(%) (%)
4.00
0 g/100ml
4.00

4.00

4.00
1 g/100ml
4.00

4.00

4.00
2 g/100ml
4.00
 4.00

4.00
3 g/100ml
4.00

4.00

4 g/100ml 4.00

4.00 3.8

4.00 3.8

5 g/100ml 4.00 3.8

4.00 3.8

4.00 3.9

5
Average Change in Length

0
0.5 1 1.5 2 2.5 3 3.5 4 4.5
Solute Concentration
 6

5
Average Change in weight

0
0.5 1 1.5 2 2.5 3 3.5 4 4.5
Solute Concentration

Conclusions
Answer your objectives in the conclusion; limit this section to 1 paragraph only with 5-7
sentences. 

References
https://sciencing.com/osmosis-experiments-potatoes-kids-12066982.html

https://www.athenology.com/post/2017/09/23/the-effect-of-osmosis-on-potatoes-in-different-
concentrations-of-sucrose-solutions