Professional Documents
Culture Documents
•
•
1 General Information
2 Theory of Operation
CELL-DYN ® 1700 SYSTEM
• 3 System Description
• 4 Circuit Description SERVICE MANUAL
• 5 Diagnostics and
Troubleshooting
• 6 Diagrams and Schematics Part Number 9211417
• 7 Removal and Replacement
• 8 Alignment and Verification
Procedures
• Appendix A Planned
Maintenance
• Appendix B Closed Sample
Option
• Appendix C CELL-DYN 1700
System Interface
Specification
Table of Contents
• Proprietary Information
• Revision Status
Abbott Laboratories software programs are protected by copyright. All rights reserved.
No part off this document may be reproduced, stored, retrieved, or transmitted in any form or by any
means without the prior written permission of Abbott Laboratories.
This service manual was developed for use in the field by trained Abbott Laboratories Field Service
Representatives. The revision status of the manual is the responsibility of the manual holder.
In no event shall Abbott Laboratories or its subsidiaries be liable for any damages incurred in connec-
tion with or arising from the use of this manual by persons not fully trained by Abbott Laboratories.
The examples contained in this manual are intended for illustrative purposes only.
The revision status of the CELL-DYN® 1700 Service Manual is indicated below. It is the responsibility
of the Field Service Representative to verify that the manual contains the latest revision number of all
pages. Additional copies of this manual may be purchased.
,
Record document control number and sign and date this log to
provide a permanent record of revisions.
GENERAL INFORMATION
Table of Contents
1.1 SECTION OVERVIEW
1.2 PURPOSE AND SCOPE
1.3 SERVICE EXPERIENCE
1.4 OPERATING INSTRUCTIONS
1.5 HOW TO USE THIS MANUAL
Service Manual Organization
Accident Prevention Labels
Biohazard Safety Precautions
Biohazard Safety
Sharps
Biohazard Disposal
Biohazard Spills
Manual Revision Information
1.6 SYSTEM SPECIFICATIONS
Physical Specifications
Operational Specifications
List of Tables
Table 1-1 Physical Specifications (Dimensions) - CELL-DYN 1700
Table 1-2 Dimensions After Packaging for Shipment - CELL-DYN 1700
Table 1-3 Physical Specifications - CELL-DYN 1700CS
Table 1-4 Dimensions After Packaging for Shipment - CELL-DYN 1700CS
Table 1-5 Power Specifications - CELL-DYN 1700 and 1700CS
Table 1-6 Printer Input Requirements
Table 1-7 Power Consumption - CELL-DYN 1700 and 1700CS
Table 1-8 Operating Environment - CELL-DYN 1700 and 1700CS
This section provides a list of the major components and sub-assemblies of the CELL-DYN 1700, a
brief description of the remaining sections in this manual, a discussion of how to use this manual, and
an overview of the instrument's physical, operational, and measurement specifications.
This manual contains service information for the CELL-DYN 1700 Automated Hematology Analyzer.
Included in this manual are the theory of operation, system and circuit descriptions, alignment and
verification procedures, diagrams and schematics, and board-level replacement procedures for all
major system components.
The CELL-DYN 1700 Automated Hematology Analyzer is a complex system. Analyzer performance
depends on several external components that together make up the complete hematology system.
Each system comprises the following components and subsystems:
• OPERATOR/OPERATOR TECHNIQUE (MAINTENANCE)
• REAGENT SYSTEM
- DILUENT
- DETERGENT
- LYSE
• PATIENT AND CONTROL SAMPLES
• ENVIRONMENT/POWER LINE INTEGRITY
• CELL-DYN 1700 ANALYZER
- SYRINGE ASSEMBLY
- FLUID POWER SUPPLY
- CLOSED SAMPLE ASSEMBLY
Based on experience and service history, the incidence of hematology problems and their causes
tend to occur in the same descending order of components and subsystems listed above. Note that
the majority of problems and their causes will originate with components external to the analyzer. It
follows that all external components and conditions, such as reagents, environment, integrity of sam-
ples and controls, etc., be checked and verified as correct before performing service on the analyzer.
In the investigation of any complaint, the instrument should be the last component of the system to be
suspected.
It is essential that the Field Service Representative read and understand the CELL-DYN 1700 Opera-
tions Manual, and be able to correctly perform all routine operating functions before attempting to
troubleshoot and repair the system.
DANGER
WARNING
CAUTION
NOTE: Denote general information and helpful hints; failure to comply will present no safety, effi-
cacy, 0 performance issues.
The Sample Prob ,electrodes, and Cap Piercing Needle (CELL-DYN 1700CS) are sharp and may be
contaminated wit potentially infectious materials. Avoid contacting them before they have been
decontaminated.
All clinical specim ns, reagents, controls, calibrators, cuvettes, and other disposables that may be
contaminated mu t be disposed according to local, state, and federal regulations governing the treat-
ment of regulated medical waste. The probe must be placed in an appropriately marked puncture-
resistant containe prior to disposal.
Biohazard S~
Clean spills of pot ntially infectious materials in accordance with established biosafety practices.
Absorb spill with absorbent material, wipe area with detergent solution, then wipe area with disinfec-
tant (100/0 solution of a 5% bleach).
I T 25 I I~I
TSB 60-025 TSB 60-025 Not
Installed Installed
The following symbol is used to identify the ISA containing additional information about the part or
area.
I 25
"NOTES" Page
Physical Specifications
Physical specifications for the CELL-DYN 1700 and CELL-DYN 1700CS are listed in Tables 1-1
through 1-7.
Analyzer Printer
Height 19" (49 cm) 5" (13 cm)
Width 34" (87 cm) 16 " (41 cm)
Depth 24" (61 cm) 14" (36 cm)
Weight 145 Ib (66 kg) 16 Ib (7 kg)
Table 1-1: Physical Specifications (Dimensions) - CELL-DYN 1700
Analyzer Printer
Height 30" (76 cm) 9" (23 cm)
Width 42" (107 cm) 22" (56 cm)
Depth 32" (81 em) 20" (51 cm)
Weight 200 Ib (91 kg) 35 Ib (16 kg)
Table 1-2: Dimensions After Packaging for Shipment - CELL-DYN 1700
CELL-DYN® 1700 Service Manual 9140265A-February1995 1-12
Section 1 GENERAL INFORMATION
Analyzer
Height 19" (49 em)
Width 34" (87 em)
Depth 26" (66 em)
Weight 155 Ib (71 kg)
Table 1-3: Physical Specifications (Dimensions) - CELL-DYN 1700CS
Analyzer
Height 30" (76 em)
Width 42" (107 em)
Depth 32" (81 em)
Weight 210 Ib (96 kg)
Table 1-4: Dimensions After Packaging for Shipment -CELL-DYN 1700CS
Setting Frequency
120 50/60 Hz
Table 1-6: Printer Input Requirements
Operational Specifications
Operational specifications for the CELL-DYN 1700 and CELL-DYN 1700CS are listed in Tables 1-8
and 1-9.
Aspiration
Open mode 30
Pre-Dilute mode 40
Closed mode 450
Table 1-10: Aspiration Volumes
Parameter CV%
WBC < 2.5
RBC < 1.7
HGB < 1.2
MCV < 1.5
PLT < 6.0
MPV < 6.0
Table 1-17: Within Sample Precision of the Hemogram Parameters - Open Mode
Parameter CV%
WBC < 2.7
RBC < 1.7
HGB < 1.2
MCV < 1.5
PLT < 6.0
MPV < 6.0
Table 1-18: Within Sample Precision of the Hemogram Parameters - Closed Mode
THEORY OF OPERATION
TABLE OF CONTENTS
2.1 SYSTEM OVERVIEW
2.2 SYSTEM DESCRIPTION
2.3 PURPOSE OF SYSTEM
2.4 SAMPLE PREPARATION
2.5 SAMPLE TRANSPORT
Open Sample Mode
Pre-Dilute Mode
Closed Sample Mode
Sample Dilution
2.6 PARTICLE DETECTION
2.7 PULSE AMPLITUDE TO PARTICLE SIZE RESPONSE
2.8 SIZE THRESHOLD AND CELL CHANNELIZATION
2.9 METERED VOLUME
2.10 RBC, WBC, AND PLT HISTOGRAM GENERATION
LIST OF FIGURES
Figure 2-1 CELL-DYN 1700 Basic Block Diagram
Figure 2-2 Electrical Impedance Detection
Figure 2-3 Pulse Amplitude to Particle Size Response
Figure 2-4 Meniscus Detection
Figure 2-5 Histogram Generation
Figure 2-6 Simplified Hemoglobin Block Diagram
This section provides a brief review of the principles of operation for the CELL-DYN 1700, including
sample preparation, sample aspiration, the flow system, and the measurement process.
The CELL-DYN 1700 is basically a particle counter dedicated to the electronic detection and mea-
surement of blood cells contained in a sample of whole blood. Blood cells are particles which exhibit
the special quality of being electrical insulators. In whole blood, these particles are suspended in a
conductive medium commonly called plasma. It is these two natural electrical qualities that permit the
electronic measurement of the following:
• The number of cells per unit of volume
• The size of each cell
• The size distribution of all cells contained in a sample.
A simplified diagram of a particle counter is shown in Figure 2-1. The major functions of an electronic
particle counter are as follows:
• Sample transport (Flow System)
• Particle detection (Transducer)
• Pulse amplitude to particle size response (Amplifier)
• Size thresholds (Discriminators) and cell channelization (AID converter)
• Sample volume metering (Metering System).
The purpose of this system is to convert the size of each detected particle to an electronic equivalent
signal. This signal is then processed to calculate the number of particles within a pre-selected size
range for a known sample volume. The displayed value represents the concentration of the sample in
cells per microliter (cellsIJlL).
Hemoglobin is measured by a separate colorimetric method. The absorbance, calculated from the
measured values of light transmission, is directly proportional to the concentration of hemoglobin.
A description of each major function of the instrument necessary to accomplish this task follows.
A major disadvantage of whole blood measurement relative to electronic particle counting is the high
concentration of cells in whole blood. This problem is easily solved by controlled dilution. A prerequi-
site for electronic particle detection is low sample concentrations that will permit the existence of only
one particle in the sensing zone at any given time. Two or more cells in the sensing zone will be
detected as a single cell and result in a counting error. The high concentration of cells in whole blood
requires accurate dilution before electronic measurement can be attempted. When the dilution ratio is
known, the value measured by the instrument can be related to the whole blood value.
An obvious question is how much dilution of whole blood is required to satisfy the requirement of sin-
gle cell detection in the sensing zone?
+VOLTS
CONSTANT M ANALOG SIGNAL
r--:-.....:...---------o I0 - - - - - - 1
AID
DATA
CURRENT I AID
GAIN
I CONY.
I ENABLE MAIN
I COMPUTER
I
_____ J
.., TRANSDUCER HARDWARE
AMP.
DISCRIMINATOR
Ro SAMPLE
+V
Ra APERTURE
r------------------------
I Metering System METERING TUBE
I
R1 DETERGENT I I
............ I OPTICAL I
I LED SWITCH I DISPLAY
I START I
I DISTANCE =VOLUME LOGIC I
OPTICAL DECODE STOP I
II ~ ~~ I
I I
I
I I
IL _ I
Sample Dilution
A 30 ,ttL sample of whole blood is drawn into the Sample Probe and mixed with 7.5 mL of diluent to
make the primary dilution. A second 100,ttL is then aspirated from the primary dilution to make the
secondary 1: 12,801 RBC/PLT dilution. The primary dilution is then mixed with 1 mL of lyse to com-
plete the 1:285 WBC/HGB dilution. The vacuum system transports the primary and secondary dilu-
tions through the WBC and RBC apertures and HGB Flow Cell.
The flow system is then flushed and made ready for the next sample.
A transducer employing the electrical impedance principle is used for detection. This function per-
forms the conversion of the physical properties of a detected cell to an electronic equivalent signal.
The continuous passage of cells through the aperture's sensing zone produces a pulse train at the
output of the amplifier. The gain control of the amplifier calibrates the sizing function of the instrument
by establishing a known relationship between the mean size of the cells and the mean pulse ampli-
tude of the signal. This linear response is depicted in Figure 2-3.
Figure 2-1, shown earlier, is a basic block diagram of the measurement and metering circuitry for
RBC, WBC, and PLT.
+ VOLTS
! t GAIN
. . ...
. .
.- . . .....
• APERTURE
SIGNAL OUT
. .
•
.. .. ... AMPLIFIER
SAMPLE· DETERGENT
TRANSDUCER
SS00007A
1.00 V
.800 V
PULSE
PEAK .600 V
VOLTS
.400 v
.200 V
The measurements require a known, repeatable sample volume. The instrument performs this func-
tion by optical detection of the leading edge of a liquid column (meniscus), as depicted in Figure 2-4.
The light transfer efficiency between an IR (infrared) light source and a photo transistor is controlled
by the optical characteristics of a glass metering tube in the light path. In the absence of liquid, as
shown in State A, the metering tube contains air and reduces the transfer of light by the refraction of
the glass walls and the density of the air within the glass tube.
With reference to State C, the metering tube is filled with liquid. The level of refraction is reduced by
an increase in optical density of the liquid, and a small increase in light transfers the results.
A third state will momentarily occur during the transition of the meniscus through the light path. As
shown in State B, the light transfer efficiency is greatly reduced when the light path is deviated by
refraction as well as reflection qualities of the concave meniscus. It is this phenomenon that serves
as a leading edge detector.
As stated earlier, each parameter has 256 channels available. The width of each channel is as fol-
lows:
RBC - 1.00 cubic microns
WBC - 1.758 cubic microns
PLT - 0.137 cubic microns
The RBC will be used as an example since it has a 1:1 relationship.
Figure 2-5 illustrates a smoothed RBC histogram and an enlarged view of the raw counts per channel
of the peak portion of the histogram (section "A").
If we compare section "B" with section "A", we can see the relationship of channel data to the actual
histogram shape. The raw counts increase, with volume, on the leading edge and decrease on the
trailing edge.
~
1 1
.-
~
"'"
.
",
./
,.-.
;'
C (WET)
LIGHT A (DRY)
TRANSFER
THRESHOLD
----------- - 1-------------
~ LIGHT REFLECTED
TIME •
Figure 2-4 Meniscus Detection SSOOOO9A
"A" "B"
SMOOTHED RBC HISTOGRAM SECTION "A" RAW COUNTS PER CHANNEL
>-
u MCV=92 >-
u
:z :z
w w
:::J SECTION A :::J
0 0
w w
0::: 0:::
LL LL
W W
> >
~
....I
~
....I
W w
0::: 0:::
50 100 150
VOLUME (CUBIC MICRONS) CHANNEL# ONE CUBIC MICRON PER CHANNEL
2.11 HEMOGLOBIN
A simplified hemoglobin system is shown schematically in Figure 2-6. The concentration of hemoglo-
bin in the prepared sample is measured in grams per deciliter. This concentration is proportional to
the absorbance of the light in the green, 540 nanometer wavelength region.
A clear reference solution (detergent) is first measured in the HGB Flow Cell. A prepared sample con-
taining hemoglobin is measured next. Hemoglobin concentration is determined by subtracting the
logarithm of the voltage of the measured sample from the logarithm of the voltage of the clear refer-
ence solution.
A light path through the transparent flow cell is formed from the light source, a 540 nanometer interfer-
ence filter, and a photo detector.
The output current from the photo detector, which is proportional to the light energy received, is ampli-
fied by the current-to-voltage amplifier and provides an output signal.
MEASURE
/REFERENCE
5V
FLOW CELL VOLTS
4V
REF 100%
I
wi
OUT LOGARITHMIC
DEI DIFFERENCE
LIGHT SAMPLE IN VOLTS =HGB
• 3V
SOURCE
AMPLIFIER 2V \.--~I-----J t
FILTER
540NM 1V '--MEASURE
SAMPLE
OV
TIME
SYSTEM DESCRIPTION
Table of Contents
3.1 SECTION OVERVIEW
3.2 SYSTEM SUB-ASSEMBLIES
Flow Panel
Fluid Power Supply
Reagent Inlet Panel
Syringe Assembly
Electronics Card Cage Assembly
CRT and Keyboard
Power Supply Module
3.3 MAJOR SUBSYSTEM DESCRIPTIONS
Data Interface and Control Subsystem
Measurement Subsystem
Solenoid and Motor Subsystem
User Interface Computer Subsystem
Data Link Adapter (DLA)
IDE Controller and I/O Board
Input/Output Board
List of Tables
Table 3-1 PC Motherboard Configuration
List of Figures
Figure 3-1 Data Interface and Control Board Diagram
Figure 3-2 Measurement Block Diagram
Figure 3-3 Solenoid and Motor Drive Block Diagram
Figure 3-4 User Interface Computer
Figure 3-5 Power Distribution Block Diagram
This section contains a system overview, as well as information on the major subsystems. For a more
detailed description of the CELL-DYN 1700 parameters, reagents, specifications, and operation, refer
to the CELL-DYN 1700 Operations Manual.
Flow Panel
The Flow Panel consists of the majority of tubing and hardware for sample processing.
Syringe Assembly
The Syringe Assembly includes the Sample Syringe for aspirating samples, the Diluent Syringe for
supplying diluent throughout the flow system, and the Lyse Syringe for dispensing lyse during the
HGB measurement process.
To aid in understanding the overall system, the electronic modules are divided into the following major
functional subsystems:
• Data Interface and Control Subsystem
• Measurement Subsystem
• Solenoid and Motor Drive Subsystem
• User Interface Computer Subsystem
• AC and DC Power Distribution Subsystem
Measurement Subsystem
The measurement subsystem provides detection, amplification, and processing of the signals from
the von Behrens RBC/PLT Transducer, von Behrens WBC Transducer, and HGB Flow Cell. RBC/
PLT and WBC metering is also included in this subsystem.
Refer to Figure 3-2 for a diagram of the measurement process.
SERIAL
USER INTERFACE UIC/CCM CCM/DCM DCM/MPM
SOFTWARE I DATA DATA BUS
DATA BUS DATA BUS
-----------l LINK
ADAPTER
USER INTERFACE COMPUTER I
I
DISPLAY MONITOR
I4---PROBE RIGHT Sw.
PC KEYBOARD ~-PROBE LEFT sw.
~-PROBE UP Sw.
I4---PROBE DOWN Sw.
CDM !+--WBC METERING MOD.
~-RBC METERING MOD.
I4---START SWITCH (OPEN MODE)
~-START SWITCH (CLOSED MODE)
~-REAGENT EMPTY SENSORS
I4---EXT. WASTE EMPTY SEN.
~-INT. WASTE EMPTY SEN.
L--_-J
I4---PRESSURE OVERLIMIT Sw.
* DENOTES LAST
TWO DIGITS
5-7
5-8
WASTE BOTTLE 2 PRESJDRAIN
LYSE INLET (NC) -n:s
::::s
OF PART NUMBER c
n:s
LYSE SYRINGE VACUUM :e
REGULATOR VAC. PUMP G)
SYRINGE VALVE
u
.~
PRM
PRESSURE " G)
SALINE SYRINGE REGULATOR PRES. PUMP tn
PROBE ROTATE
,--
I
PRES. PUMP (0.8)
Q
Q
......
~
@
PROBE UP/DOWN
M Z
C >-
CI
o SAMPLE SYRINGE
...J
~G) Figure 3·3 Solenoid and Motor Drive Block Diagram SS00036A
...J
W
tn o
Section 3 THEORY OF OPERATION
In addition to the motherboard, the User Interface Computer consists of the following components:
1. Data Link Adapter to interface with Cell Count Module
2. IDE Controller (for floppy and hard disk drives) and I/O board
3. InpuUOutput board
4. Super VGA board
5. Computer monitor
Figure 3-4 illustrates the major components of the User Interface Computer.
Data Link Ada.,pter (DLA).
The Data Link adapter provides interfaces from the PC motherboard to both the Cell Count Module
and the instrument key panel. Refer to Section 4 for a detailed description of the DLA.
IDE Controller and I/O Board
The IDE (Integrated Device Electronics) controller and 1/0 (InpuUOutput) board contains the interface
to both the hard and floppy disk drives, one serial port, and one parallel port. The cable to the hard
disk drive is a 40-pin connector and the cable to the floppy disk drive is a 34-pin connector. The board
must be jumpered so that the serial port is COM 1 and the parallel port is LPT1. If applicable, the
graphics printer must be connected to LPT1.
NOTE: If a LIS (Laboratory Information System) is installed, it must be connected to the COM1 port.
The standard I/O board is a PC-AT type board with a serial port and parallel port. The serial port must
be jumpered to COM2 and the parallel port must be connected to LPT2. If applicable, the ticket
printer must be connected to LPT2.
Super VGA Board (SVGA)
The super VGA board operates in standard VGA mode: It has a 16 color capability and a standard
15-pin VGA connector. It must be plugged into a 16-bit slot. The board has no adjustment.
Computer Monitor
The computer monitor is a custom 14" color monitor from ELECTROHOME. It operates in 16 color
mode with a resolution of 640 x 480. The monitor has special shielding and the display adjustment
controls are mounted on the front directly below the screen. The controls from left to right are contrast,
brightness, height adjustment, screen position adjustment, and screen width adjustment.
The monitor has a built-in cable that is attached to the SVGA board. AC power comes directly from
the Linear (main) Power Supply.
DATA LINK TO
PC ANALYZER SUBSYSTEM (CCM)
POWER SUPPLY
..4 ..
HARD DISK
DRIVE
DATA LINK
PC MOTHERBOARD ADAPTER
FLOPPY DISK
DRIVE
PANEL
KEYBOARD
PARALLEL PORTS PC
SERIAL PORTS
KEYBOARD
I
SPARE
RS-232
COMM
TICKET
PRINTER
GRAPHICS
PRINTER SPEAKER SVGABOARD - DISPLAY
MONITOR
TO SOLENOID
DRIVE BOARDS +24V
SWITCHER
REGULATED
STEPPER
+ 12 UNREG & +24 UNREG CABLE MOTORS
DISTRIBUTION
MODULE ADJUSTABLE
LINEAR + 100 - ... PRE-AMP BOARD
+ 5
PC
POWER
SUPPLY
- 5
+ 12
- 12 o +24V
MOTOR
FLOPPY DISK DRIVE PROCESSOR
PC MODULE
MOTHERBOARD +5V
HARD DISK DRIVE TO DIGITAL
LOGIC BOARDS
Figure 3-5 Power Distribution Block Diagram (STEPPER MOTORS) SS00016A
CIRCUIT DESCRIPTIONS
Table of Contents
4.1 SECTION OVERVIEW
4.2 PRE-AMPLIFIER MODULE (PAM)
4.3 MAIN AMPLIFIER MODULE (MAM)
4.4 SIGNAL PROCESSOR MODULE (SPM)
4.5 CELL COUNT MODULE (CCM)
Microprocessor Section
System Clock
6809E Microprocessor Support Circuits
PROM
RAM
VIA (Versatile Interface Adapter)
DMA (Direct Memory Access) Section
Pulse Height Memory (PHM)
Cell Counters
DMA Timing and Control
Interface to Microprocessor Bus
4.6 METERING MODULE
List of Figures
Figure 4-1 Cell Pulse Classifications
Figure 4-2 MAM (Main Amplifier Module) Block Diagram
Figure 4-3 SPM (Signal Processor Module) Block Diagram
Figure 4-4 DLA (Data Link Adapter) Block Diagram
This section contains a description of the circuitry for the following printed circuit boards:
• Pre-Amplifier Module (PAM)
• Main Amplifier Module (MAM)
• Signal Processor Module (SPM)
• Cell Count Module (CCM)
• Metering Module
• Device Control Module (DCM)
• Cable Distribution Module (CDM)
• Solenoid Driver Module (SDM)
• Motor Processor Module (MPM)
• Stepper Drive Printed Circuit Board (SDP)
• PressureNacuum Regulator Module (PVRM)
• Linear Power Supply Module
• PC Power Supply Module
• 24V Switching Module
• Pump Relay Module (PRM)
• User Interface Computer (UIC)
• Data Link Adapter (DLA).
Refer to Figure 3-2 in Section 3 and Figures .4-1 and 4-2 in this section.
The Main Amplifier Module consists of the following major circuits:
1. WBC differential amplifier and main amplifier
2. RBC/PLT differential amplifier and main amplifier
1
HEIGHT (H)
B •
APERTURE
/
-\)~\.)~\.)"""'-------+.
1 AREA (A)
A B
TUMBLING
C- •• - - - - - - - . NORMAL
A .------- .
o .-------.
C D
COINCIDENCE EDGE EFFECT
u
U) It)
w en
C OFFSET en
-'\M" or-
-::::»
I- R11
GAIN 1 ~
«J
u GAIN 2
::s
-UD:::
L.
.c
CI)
RBCANALOG u.
~
CO
N
Q
oqo
0r-
PLTANALOG~ en
1JU
PEAKDET.
LATCH
U21
RESET
-::s
«J
~. I ONE SHOT I
U20
• lONE SHOT I
U20
• c
«J
:E
CI)
RESET Co)
.~
CI)
U)
"» • I U24~ • Q
Q
INTEGRATOR to-
or-
TP15 INTEG OUT @
z
~
o
c is•
..J
~
CI)
Figure 4-2 MAM Block Diagram SS00035A
..J
W
U) U
Section 4 CIRCUIT DESCRIPTIONS
WBCANALOG
WBCS&H ,
RBCANALOG
RBCS&H 6 "-
,/
ANALOG
PEAK
..() INPUT 5 "-
- U2 "- 9BIT ,/
DETECTOR AID
?I 4 "-
U1
RBC DISC. - ,/
- U4)-
U6~ 3 "-
CURRENT SELECT r U4 ) - LATCH
U12
'---
,/
2 "-
,/
PLTANALOG PLTUPPER ---4
DISC. 1
U3 ~~
"-
- ,/
PEAK U4 ""- 0
DETECTOR r-- ../ 0 "-
,/
PLTLOWER - -EOC
DISC. "-
CELL STROBE ,/
PLTS& H
Figure 4-3 SPM Block Diagram SSOOO18A
Microprocessor Section
System Clock
The CCM uses an a MHz oscillator (U10) that is divided by eight by a Johnson counter (U20) to pro-
vide 1 MHz system clocks for the 6aOgE microprocessor. The signals E and Q are provided to the
6aOgE by the Johnson counter. VUA (Valid User Address) is provided to pin 10 on the motherboard.
The EPROM used on the CCM (U37) is a 27C256, for 32K by 8 of program storage. A strappable
jumper selects the EPROM type.
RAM
The program RAM used on the CCM (U36) is a HM6264" (or equivalent), for 8K by 8 of program data
storage. A strappable jumper selects the RAM type. Jumper J5 must be set to 8K.
VIA (Versatile Interface Adapter)
The LSI (Large Scale Integration) interface used on the CCM is a 6522 VIA (U13). This device per-
forms a number of functions, as described below:
1. CCM Real Time Clock
One of the two 16-bit timer/counters in the VIA is used for the CCM real time clock. This time
base is always programmed to 1 millisecond (in current applications); it presents a repetitive
FIRQ interrupt to the 6809E. All process control functions, e.g., flow system timing, stepper
motor motions, sensor scanning rates, etc., are based on this timer. There should always be a
1 KHz frequency at test point TP5.
Two HM6116 2K by 8 static memory devices (U15 and U14) are used to store the pulse height
counts. The two memory devices are electrically set up as 2K bytes by 16 bits of addressable mem-
ory. Furthermore, this memory is divided into four functional blocks of 512 16-bit words. In normal
operation, these blocks hold the WBC, RBC (low current), Platelet, and RBC (high current) counts per
channel.·
The PHM is unique in 2 important ways. First, the CCM program can only read the memory or clear
the memory, it cannot store values into the memory (except for zero, by clearing it). Second, the CCM
program is blocked from reading the PHM memory while a DMA is in progress.
The DMA circuit, on the other hand, can read and write the memory directly, but it can only transfer
data to and from the
16-bit cell pulse counter.
Read/write control of the PHM is performed by an LS158 (U18), which acts as a DMA / MPU address
selector.
Cell Counters
The cell count values stored in the PHM are incremented by the four 4-bit counters (U29, U28, U27,
U26). These counters are cascaded and employed as a 16-bit pre-settable synchronous counter.
9140265A-February1995 4-19
CELL-DYN® 1700 Service Manual
Section 4 CIRCUIT DESCRIPTIONS
Vacuum and pressure control data is latched by U14 and routed to the Pump Relay Module via J11.
Pump status signals (Vac On, Pres On) are converted to TTL levels by U3 and placed on the data bus
by U5.
lEO drive signals are routed to the Start Board via J17. The start signal enters at J17 and is placed
on the data bus by U5.
Refer to blocks 20, 23, and 24 off the MPM in Figure 3-3 in Section 3.
The Stepper Drive printed circuit board consists of two PBL 3717 motor drive chips. Each chip drives
a winding of the Stepper Motor. Bits 10 and 11 are used to control four motor current levels:
1. PO - High Current
2. P1 - Medium Current
3. P2 - Low Current
4. P3 - Current Off
Bits PHO and PH1 control motor phase and, therefore, direction and step-rate (velocity). Feedback+
and Feedback- are used to generate a motor self-test.
The Linear (Main) Power Supply module get:lerates the following voltages:
1. +12VDC - Cable Distribution Module
2. +24VDC - Cable Distribution Module
3. +100VDC - Pre-Amp Module
4. +15 & -15VDC - Analog Circuit Boards
Refer- to Figure 3-5 in Section 3.
9140265A-February1995 4-24
CELL-DYN® 1700 Service Manual
Section 4 CIRCUIT DESCRIPTIONS
The voltages generated on the PC Power Supply are routed to their final destinations by the PDM
(Power Distribution Module).
Refer to Figure 3-5 in Section 3.
The +24VDC Switching Module regulates power to the Motor Processor Module, via the PDM, to drive
the Stepper Motors. Refer to Figure 3-5.
The User Interface Computer (UIC) is designed around an Intel-compatible 386SXlDX chip running at
25 - 40 MHz. (Future configuration may be a 486DX chip running at 33 or 66 MHz.) The UIC
receives power from the' Switching Power Supply via the Power Distribution Module and receives sta-
tus and measurement data from the CCM. The board contains EPROM, CMOS RAM, input/output
circuitry for the interface ports, and addressing circuitry.
The following components, described in Section 3 of this manual as part of the User Interface Com-
puter, are purchased OEM from commercial vendors:
The Data Link Adapter provides interfaces from the 386/486 computer to both the CCM (Cell Count
Module) and the instrument (membrane) key panel. These two interfaces function independently,
under application software control. However, some of the circuits on the DLA board are shared. The
DLA uses an 82C55 PPI (Parallel Peripheral Interface) IC. Refer to Figure 4-4 for an illustration of the
DLA board.
Interface to CCM
A 20-pin ribbon cable connects the DLA to the CCM. This interface is a bi-directional, parallel inter-
face that is software-controlled at both ends. Data is transferred in 8-bit bytes on 8 data lines (DO-
D7) in one direction at a time. The UIC (User Interface Computer) initiates an inquiry message "hand-
shake on a periodic basis or when it has a command to send. The CCM responds by sending data or
replying to the command sent.
IRQ
INTERRUPT
CONTROL CONN
TO
CCM
AEN
82C55
PPI
PORTB
I 8BIT DATA BUS - PORT B
PORTC
lOW CONN
BOARD TO
lOR SELECT KEY
LOGIC PANEL
4 BIT BUS MATRIX
ADDRESS
AO-A9
PC BUS
CONN - (16 BIT)
Figure 4-4DLA (Data Link Adapter) Block Diagram SS00019A
Table of Contents
5.1 SYSTEM OVERVIEW
5.2 DIAGNOSTICS MENU USAGE
Level One
Level Two
Level Three
Level Four
5.3 FAULT REPORT DESCRIPTION
5.4 CELL-DYN 1700 TROUBLESHOOTING GUIDE
Troubleshooting Chart
Nonfunctional Instrument Problems
Video Display Problems
Displayed Error and Fault Problems
Data Problems
Clog and Flow Error Problems
Miscellaneous Problems
5.5 RAW DATA DESCRIPTION
Raw Data DisplayDescription
List of Tables
Table 5-1 Disk Drive Setup Information
Table 5-2 DEC Service Commands
Table 5-3 Motor Power Specifications
Table 5-4 Motor Direction Commands
Table 5-5 Motor Speed Commands
Table 5-6 Error Messages
Table 5-7 Event Messages During Diagnostic Menu Count Test
Table 5-8 Operator-Correctable Alarm or Fault Messages
Table 5-9 Summary of Error Messages
List of Figures
Figure 5-1 Smoothing ON/OFF Example
Figure 5-2 Raw Data Example
Figure 5-3 AMI BIOS
Figure 5-4 Motor Power Test
Figure 5-5 Probe Up/Down Initialize and Run Cycle
Figure 5-6 Probe Initialize Mode
This section is designed to aid the service representative in the troubleshooting and repair of the
CELL-DYN 1700/1700CS System. Emphasis is placed on using various System Status and Fault
Reports, which can be accessed by the service representative, to solve problems. Special Service
Commands are also available to exercise and observe mechanical and electronic functions.
Utilization of the DIAGNOSTICS Menu enables the operator or service representative to identify and
correct both operator-correctable and service-correctable faults. When the computer senses a fault,
the message <NOT READY: SEE DIAGNOSTICS> is displayed in the System Status Box on the
RUN Menu. The following keys are available in the DIAGNOSTICS Menu.
Level One
Level Three
• PROBE HOME: Moves Sample Probe up and above RBC Cup.
• PROBE UP: Moves Sample Probe up.
• MORE: Used to display additional functions.
• PRINTER OUTPUT: Used to toggle printer output ON and OFF.
9140265A-February1995 5-7
CELL-DYN® 1700 Service Manual
DIAGNOSTICS AND TROUBLESHOOTING
Sec tion 5
0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0
1 55 53 50 49 50 52 53 50
0 0 0 0 86 84 69 58
17 59 63 64 70 70 73 74 75
51 53 55 55 57 58 58 59
33 85 88 84 84 83 86 86 86
77 76 79 82 82 84 84 84
49 98 98 .100 98 96 96 97 97
87 89 90 91 94 96 99 99
65 72 69 68 69 67 65 62 61
93 88 87 81 78 76 75 72
81
6 6 5 5 0 0 0 0 0
9 9 9 9 8 8 6
241
Peak of 100 at chann el 75
A detailed list of all faults generated by the CELL-DYN 1700 software and hardware is contained in
Section 5.10. The fault classifications reported in the Fault Report primarily contains data pertaining
to the last CCM fault.
If a fault occurs, pressing the [HELP/ERROR] key will immediately display the Fault Log in the DIAG-
NOSTICS Menu. This log may contain up to 16 faults, with the current fault leading the list. An alter-
native procedure is to go to the MAIN MENU and press [DIAGNOSTICS]. In this case, the Fault
Report, not the Fault Log, is immediately displayed.
9140265A-February1995 5-9
CELL-DYN® 1700 Service Manual
DIAGNOS TICS AND TROUBLESHOOTING
Sec tion 5
MENU press [DIA GNO STIC S] fol-
To view the Fault Log when no fault is pending, from the MAIN
up to 16 past faults. Press [FAU LT
lowed by [HEL P/ER ROR ] and [HE LP]. The system will display
<NO FAU LTS OR WAR NIN GS
REPORT] to display the FAULT REPORT screen. A display of
PENDING> indicates that all faults have been cleared.
9140265A-February1995 5-11
CELL-DYN® 1700 Service Manual
·.·...·,.•.,·.t.'.·...
2. DEFECT. VDM
8. INCORRECT 1. MISADJUST.
HORIZ. WIDTH
9140265A-February1995 5-12
CELL-DYN® 1700 Service Manual
DIAGNOSTICS AND TROUBLESHOOTING
Section 5
CORRECTIVE ACTION
SYMPTOM
1. REPLACE VDM
1. ADJUST FRONT
9. CHARACTERS
CONTROLS
OUT OF FOCUS
2. DEFECT. VDM 1. REPLACE VDM
2. DEFECTIVE
SENSOR 1. CHECK SENSOR
2. REPLACE SENSOR
9140265A-February1995 5-14
CELL-DYN® 1700 Service Manual
DIAGNOSTICS AND TROUBLESHOOTING
Section 5
CORRECTIVE ACTION
SYMPTOM
3. PRESSURE 1. CHECK SOL 3-3
OVERLIMIT 2. REPLACE SOL 3-3
9140265A-February1995 5-15
CELL-DYN® 1700 Service Manual
Section 5 DIAGNOSTICS AND TROUBLESHOOTING
SYMPTOM PROB. CAUSE CORRECTIVE ACTION
6. WASTE OVRFL 1. NO AIR PRES. 1. CHECK PRESSURE
INTO ACCUM 2. REPLACE PUMP
3. REPLACE CDM
2. SOL 5-3 STUCK 1. CHECK SOL 5-3
2. REPLACE SOL 5-3
3. SOL 5-7 STUCK 1. CHECK SOL 5-7
2. REPLACE SOL 5-7
4. SENSOR NOT
DETECTING 1. CHECK SENSOR
2. REPLACE SENSOR
3. REPLACE CDM
7. WASTE EMPTY 1. DEFECTIVE
TIMEOUT SENSOR 1. CHECK SENSOR
2. REPLACE SENSOR
2. AIR PRESSURE 1. CHECK PRESSURE
LOW 2. REPLACE PUMP
3. RESTRICTION 1. CHECK PLUMBING
4. DEFECT. CDM 1. REPLACE CDM
From the MAIN MENU, press [DIAGNOSTICS] followed by [RAW DATA]. The [RAW DATA] key will
display raw data obtained from the last count cycle.
When a single count is done, all data is contained in the first column. When a PLT recount occurs,
data from the first cycle appears in column #2 and data from the recount appears in column #1.
The seven LEOs on the CELL-OYN 1700 CCM can reveal much about the fundamental CCM and
overall machine state. In general, the LEOs indicate whether the CCM is in a normal functioning
mode or in a fault state, and in either case, help to characterize the CCM state. Also, one of the LEOs
gives some information about the state of the UIC as well.
The CCM tests itself on power-up. These fundamental tests include ROM, RAM, and VIA. If any test
fails, the CCM will attempt to execute a routine which will flash the green LED on the board. Also, it
will place a 4-bit fault code into the adjacent yellow LEOs.
WBC Count 51
RBC Count 2 RBC RER: 100.0%
PLT Count 6
Hgb Error o
Hgb Reference 2048
Hgb Sample 2045
WBC Up time 1985 WBC Upper max 1985 Upper min 1985
WBC Cnt time 4752 WBC Time-out 6500 Avg. time 4752
RBC Up time 5171 RBC Upper max 5171 Upper min 5171
RBC Cnt time 6854 RBC Time-out 7500 Avg. time 6854
Flow Error 0000
LED is off
green LED on, not flashing
green LED flashing slowly (approx. 1 Hz)
yellow LED on, not flashing
yellow LED fast-flickering
yellow LED flashing at slow, non-periodic rate
Power-on Tests
1. gs - - - y - y CeM failed ROM test, on 1st checksum byte.
2. gs - - y - - Y CCM failed ROM test, on 2nd checksum byte.
3. gs - - y y - Y CCM failed VIA test, register checked
(DDRA).
4. gs - Y - - - Y CCM failed VIA test, register checked (IER).
5. gs - Y - Y - Y CCM failed VIA test, register checked (IFR).
6. gs - Y Y - - Y CCM failed VIA test, register checked (VCR).
7. gs - Y Y Y - Y CCM failed VIA test, register checked (DDRB).
8. gs Y - - - - Y CCM failed RAM test, walking 1IS.
9. gs Y - - Y - Y CCM failed RAM test, on clearing to zero.
CMOS Setup
The CMOS Setup contains all the information needed by the BIOS system to establish proper com-
munications between the motherboard and the various computer system devices. The configuration
for the current motherboard is listed below:
Part No.
Current motherboard 1700019
IDE hard drive 2005712
IDE controller 1700021
Floppy
Disk Drive NA NA NA NA NA 1.44
3.5"
Setup Screen
The Setup screen is the user interface to the Basic Input-Output System (BIOS) which resides on the
battery backed-up CMOS RAM chip on the motherboard. The Setup screen is used to input the hard-
ware configurations of the various devices, so that the BIOS can set up proper communications within
the computer.
The BIOS currently shipped on the CELL-DYN 1700 was developed by American Megatrends Inc.
(AMI). The AMI BIOS setup is illustrated in Figure 5-3.
Discussion
Several commands are available to initiate individual actions in the CELL-DYN 1700 hardware and
software. These commands are used for troubleshooting and/or alignment when a single action is
desired or required to be repeated several times.
The special command mode resides in the DIAGNOSTICS Menu. From the MAIN Menu, press
[DIAGNOSTICS] followed by [SERVICE DEC CODE]. When this softkey is pressed, the message
<SERVICE FUNCTION ONLY: ENTER COMMAND:> is displayed.
A command can now be entered. Pressing the Enter key on the keyboard will initiate the action. Only
one command can be entered at a time and [SERVICE DEC CODE] must be pressed before a com-
mand is entered.
All commands available by direct softkey can be accessed by pressing [MORE].
NOTE: Use only the commands listed in Table 5-2 and always verify that the correct number has been
entered before initiating the action. Use only those numbers listed below. Other numbers may
CELL-DYN® 1700 Service Manual 9140265A-February1995 5-33
Section 5 DIAGNOSTICS AND TROUBLESHOOTING
refer to engineering commands which are not to be used in the field and which may cause dam-
age if used improperly. Be fully aware of the purpose of any of the following commands before
using them. This is a direct-activation method which should be used with caution because the
physical state of the CELL-DYN 1700 may not be in agreement with the function to be per-
formed. After using service commands, always re-initialize the system by turning the power
OFF then ON again or by pressing the [INITIALIZATION] key in the DIAGNOSTICS Menu to
ensure the instrument is in the proper configuration for normal operations.
Options:
Enabled: Daylight saving ON
Disabled: Daylight saving OFF
Typematic Rate Programming : Disabled : Adaptor ROM Shadow 0800, 32K : Disabled
Typematic Rate Delay (msec) : 500 : Adaptor ROM Shadow EOOO, 32K : Disabled
Typematic Rate (Chars/Sec) : 15 : Adaptor ROM Shadow E800, 32K : Disabled
Above 1 MB Memory Test : Enabled : System ROM Shadow FOOO, 64K : Enabled
Memory Test Tick Sound : Enabled : RAS Time Out : Enabled
Memory Parity Error Test : Enabled : 16bit ISA Cycle Insert Wait : 1 W/S
Hit (DEL) Message Display : Enabled : RAS Active Time Insert Wait : Disabled
Hard Disk Type 47 Data Area : 0:300 : Quick RAS Percharge Time : Disabled
Wait For (F1) If Any Error : Enabled ; Slow Refresh : 600 us
System Boot Up Num Lock ; On : 10 Recover Period Define : Enabled
Numeric Processor : Absent : CAS Percharge Time Wait : Disabled
Floppy Drive Seek At Boot : Disabled : Memory Write Insert Wait : Disabled
System Boot Up Sequence : C:, A: : Memory Read Insert Wait : Enabled
Internal Cache Memory : Disabled
Password Checking Option : Disabled
Video ROM Shadow COOO, 32K : Enabled
Adaptor ROM Shadow C800, 32K : Disabled
Adaptor ROM Shadow 0000, 32K : Disabled
Typematic Rate Programming : Disabled : Adaptor ROM Shadow 0800, 32K : Disabled
Typematic Rate Delay (msec) : 500 : Adaptor ROM Shadow EOOO, 32K : Disabled
Typematic Rate (Chars/Sec) : 15 : Adaptor ROM Shadow E800, 32K : Disabled
Above 1 MB Memory Test : Enabled : System ROM Shadow FOOO, 64K : Enabled
Memory Test Tick Sound : Enabled : BootSector Virus Protection : Disabled
Memory Parity Error Test : Enabled : Memory Wait State : Disabled
Hit (DEL) Message Display : Enabled : RAS Time Out : Enabled
Hard Disk Type 47 Data Area : 0:300 : l6bit ISA Cycle Insert Wait : 1 W/S
Wait For {F1} If Any Error : Enabled : RAS Active Time Insert Wait : Disabled
System Boot Up Num Lock : On : Quick RAS Percharge Time : Disabled
Numeric Processor : Absent : Slow Refresh ; 600 us
Floppy Drive Seek At Boot : Disabled : 10 Recover Period Define ; Enabled
System Boot Up Sequence : C:, A: : CAS Percharge Time Wait : Disabled
Internal Cache Memory : Disabled : Memory Write Insert Wait : Disabled
Password Checking Option : Disabled : Memory Read Insert Wait : Enabled
Video ROM Shadow COOO, 32K ; Enabled ; Cyrix KENT Timing : Disabled
Adaptor ROM Shadow C800, 32K : Disabled : Cyrix Shadow BIOS Cacheable : Disabled
Adaptor ROM Shadow 0000, 32K : Disabled : Memory Remap Select : Enabled
Under Advanced CMOS Setup, pressing the F6 key displays the default settings. The settings
displayed in Figure 5-3 above are set at default except for the bolded settings, which have been
changed for the CELL-DYN 1700.
The normal system bootup sequence is drive C:, A:. During instrument service, if it is neces-
sary to boot from the floppy disk, change the bootup sequence to A:, C:. When service is com-
plete, the sequence must be changed back to C:, A:.
Auto-CyclingjCode 999)
The motors that enable the Sample Probe to move up/down and to rotate are stepper motors which
are under direct computer control. Since there is no direct positional feedback sent to the computer,
position switches are employed to verify critical positions during normal operation. It is important to
understand that these switches only verify and do not control the movement of the Sample Probe.
In the DIAGNOSTICS Menu, Service DEC Codes 128, 129, and 130 allow the service representative
to control and exercise all stepper motors in the CELL-DYN 1700. This description will focus on the
Probe Up/Down Motor (B/2) and the Probe Rotate Motor (C/3) which control the movement of the
Sample Probe.
Speed in Steps
Command
per Second
1 50
2 75
3 283
4 300
5 166
6 200
7 250
8 10
9 151
10 222
11 25
12 182
13 100
CELL-DYN® 1700 Service Manual 9140265A-February1995 5-52
Section 5 DIAGNOSTICS AND TROUBLESHOOTING
Table 5-5: Motor Speed Commands (Continued)
Motor Speed Commands
14 125
15 91
16 67
17 111
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Figure 5-5 Probe Up/Down "INITIALIZE" and "RUN" Modes SSOOO29A
tn o
Section 5 DIAGNOSTICS AND TROUBLESHOOTING
AS PIRATIO N_--7'1"'"--"""
---r HARD STOP
117 STEPS
112 STEPS
CENTER POSITION
HARD STOP
3
ADJUST
SW. #3 HERE DISPENSE POSITION
DISPENSE POSITION
119
112
CENTER POSITION
Position Fault
Fault data:
00:04 00 OA DC 31 00 00 00 DO 1E 31 FF 00 00 00
00
10:00 00 00 01
Cksum: 19 31 I 22 bytes received
Process number: 49
Step number at time of fault: 4
Process elapsed time: 2780 ms.
Switch: 1 , check
Position Fault
Fault data:
00:02 00 00 52 35 00 OC E4 ED CD 32 FF 00 00 00
00
10:00 00 00 01
Cksum: 32 EB I 22 bytes received
Process number: 53
Step number at time of fault: 2
Process elapsed time: 3410 ms.
Switch: 2 . check
Position Fault
Fault data:
00:07 00 19 FO 31 00 02 1C DO 77 33 FO 00 00 00
00
10:00 00 00 01
Cksum: 78 34 I 22 bytes received
Process number: 49
Step number at time of fault: 7
Process elapsed time: 6640 ms.
Switch: 3 check
«
On switch(es) 2: top
Position Fault
Fault data:
00:00 00 7.c 38 30 00 00 00 FO 02 34 FD 00 00 00
00
10:00 00 00 01
Cksum: CD AE I 22 bytes received
Process number: 48
Step number at time of fault: 13
Process elapsed time: 31800 ms.
SWitch;A . cbeck
On switch(es) 2: top
Table 5-6 below lists the most serious error messages on the CELL-DYN 1700 instrument.
The user has entered a command for the CCM whose numeric value
Code N is
exceeds 127. The value entered was N.
Invalid
Incomplete On a CELL-DYN 1700CS, not enough blood was detected for the
Aspiration last processed sample.
Error Message
Description
(Status box)
Cannot do this The user has attempted to issue a command to the CCM that cannot
Function be executed because of a pending fault condition.
WBC Meniscus
Detection, RBC During the most recent count, a meniscus was not detected or was
Meniscus detected at an unexpected time.
Detection
WBC Count
Time-out (clog),
During the most recent count, a Clog occurred.
RBC Count
Time-out (clog)
Other error-
related mes-
sages:
An error in CCM/MPM interprocessor communications occurred. A
fault was generated in an attempt to send or receive motor or other
MPM to CCM,
command to or from MPM, or the MPM was unable to perform the
Message Trans-
function.
mit Error
Command to
be Sent to
MPM is Incor-
rect
Error Message
Description
(Status box)
Count Test
The [COUNT TEST] key in the DIAGNOSTICS Menu is used to run specimens and display Count
Check data without returning to the RUN Menu. Coded data relating to specific cycle functions, raw
measurement, and flow count time are displayed for use in troubleshooting or service.
Table 5-7 lists the event messages that are displayed during the Diagnostic Menu Count Test.
DOS Errors
Arithmetic overflow
Bad drive request structure length
Cannot remove current directory
Cannot rename across drives
Collection index out of range
Collection overflow
CRC error in data
General
System Fault
Not Ready: See DIAGNOSTICS
Uninitialized
Normal Operator-Correctable
Detergent empty
Diluent empty
Lyse empty
Waste full
DLA Faults
Date
Battery failure or system date/time not set
Disk I/O
Cannot open CD1700. ini configuration file
QC Log
Can not accept specimen
Can not reject specimen
No loading, QC file has to be empty
Purge log failed
Read QC file failed
Data Log
Cannot do if uninitialized
Cannot write Data Log header to disk
Count overrange
Data Log write error
Failed to read from Data Log
Flow err
Initialize
Data Log initialization failed
QC Log initialization failed
Communications
Break interrupt
Comm open error
Comm error
Framing error
Printer Driver
Printer Fault
Printer Not Ready
Printer Off-line
Printer Out Of Paper
Printer Time-out
Ticket Printer Not Ready
QC Log I/O
Failure to read from QC Log
Failure to write to QC Log
Help
Unable to open help file
Reagent Log
Unable to create file
Unable to open file
X-B File
Failed to write to Data Log Header
Failed to write to Data Log
No response from CCM
General Faults
Abnormal time-out / no MPM response
Attempt to send MPM a new command while busy
CCM pulse height memory saturation warning
Fault Log
Error in writing to Fault Log file
Error in writing header to Fault Log file
Lab ID Setup
Drive A is not ready
Fail to read assay file
Fail to write LAB ID file
Incorrect assay format
Incorrect disk. QC values do not apply to this instru-
ment
Incorrect Exp. Date
Incorrect Lot Number
Incorrect parameter in assay file
CD1700 (subdirectory)
CD1700.EXE
CD1700.INI
DATALOG (20.5 MB)
QCLOG (5.5 MB)
FAULTLOG
REAGLOG
ID.DAT
HELP (subdirectory)
HELPxx (xx is numeric)
Accessing DOS
There are two ways to access the DOS program from the CD1700 program: with the CD1700 pro-
gram still loaded or by exiting the CD1700 to DOS. The Exit CD1700 to DOS method is the preferred
method.
Exiting DOS
To exit the DOS Program and return to the CD1700 Program in the case where the CD1700 Program
remains loaded (Alt d was used to access DOS), type exit at the C prompt and press the Enter key.
The DIAGNOSTICS Menu will again be displayed.
For example, to stop the file names at the end of each page: type dir /p and press the Enter
key.
To display the file names in columns across the width of the page: type dir /w and press the
Enter key.
Software Installation/Upgrades
1. 1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.
2. 2. Press ESC on the PC keyboard. A warning message appears on the screen. Heed
the message.
3. 3. Press the Alt and x keys simultaneously. The following DOS prompt appears:
C:\CD1700>.
4. 4. Install the software disk in Drive A.
5. 5. For initial software installation only, type A:\ncs for the 1700 model or A:\cs for the
Table of Contents
6.1 SECTION OVERVIEW
This section contains diagrams and schematics for the CELL-DYN 1700 and 1700CS.
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CELL-DYN® 1700 Service Manual 9140265A-February1996 6-4
Section 6DIAGRAMS AND SCHEMATICS
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CELL-DYN® 1700 Service Manual 9140265A-February 1996 6-12
Section 6DIAGRAMS AND SCHEMATICS
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CELL·DYN® 1700 Service Manual 9140265A-February 1996 6-15
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CELL·DYN® 1700 Service Manual 9140265A-February 1996 6-21
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CELL-DYN® 1700 Service Manual 9140265A-February 1996 6-25
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CELL-DYN® 1700 Service Manual 9140265A-February 1996 6-27
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9510056 (SMeI 2 0(2)
Table of Contents
7.1 INTRODUCTION
7.2 SAFETY PRECAUTIONS
Decontamination
Decontamination Procedures
Printed Circuit Board Handling
7.3 SERVICE EQUIPMENT REQUIRED
7.4 DISASSEMBLY/REPLACEMENT PROCEDURES
Model 1700CS Upper Front Cover Removal
Model 1700 Upper Front Cover Removal
Model 1700CS Lower Front Cover Removal
Model 1700 Lower Front Cover Removal
Top Cover Removal
Bezel Removal
Right Cover Removal
Left Cover Removal
RBC and WBC Aperture Plate Removal
von Behrens WBC and RBC/PLT Transducer Removal
WBC and RBC Metering Board Removal
This section contains warnings and cautions that must be followed for
your protection and to avoid damage to the equipment.
Before any repair is completed, make sure that all safety features are
intact and functioning and that all grounded parts are connected to
their proper grounding terminals.
The CELL-DYN 1700 instrument, in common with all medicallabora-
tory instrumentation, can be exposed to biohazardous material during
normal use. Correct laboratory procedures should be followed and pre-
cautions taken to reduce the potential for contamination. To reduce the
risk of biohazard exposure, perform the following decontamination pro-
cedure prior to servicing the instrument.
Chemical Hazards
IMPORTANTI Before you operate, service or perform maintenance on
any equipment, be sure to read and understand all the information con-
tained in the current Material Safety Data Sheets (MSDS).
Always wear proper personal protective equipment and follow correct
work procedures as specified in the OSHA Hazard Communication
Standard (29 CFR 1910.1200).
MOdel 1700CS Upper Front Cover Removal 3. Remove the upper front cover by pulling it up about 1J4-inch
To remove: and out. (See Figure 7-2).
1. Loosen the thumbscrew holding the Closed Sample Assembly
to the front of the instrument. (See Figure 7-1).
Figure 7-2
4. Disconnect the ground wire. (See Figure 7-3).
Figure 7-1
2. Swivel the Closed Sample Assembly out until it is clear of the
covers.
Figure 7-3
Model 1700 Upper Front Cover Removal Model 1700CS Lower Front Cover Removal
To remove: To remove:
1. Remove the upper front cover by pUlling it up about 1J4-inch 1. Remove the upper front cover (page 7- 4).
and OUt. (Refer to Step 3 of the Model 1700CS Upper Front
Cover Removal procedure on page 7-4.) CAUTION: Be sure the Sample Probe is out of the way before at-
2. Disconnect the ground wire. (Refer to Step 4 of the Model tempting to remove the lower front cover to prevent damage to the
probe.
1700CS Upper Front Cover Removal procedure on page 7-4.)
Figure 7-4
4. Slide the lower front cover to the left approximately 1f4-inch until it Model 1700 Lower Front Cover Removal
clears the bezel, then lift up and out. (See Figure 7-5).
To remove:
1. Remove the upper front cover (page 7-5).
CAUTION: Be sure the Sample Probe is out of the way before at-
tempting to remove the lower front cover to prevent damage to the
probe.
Figure 7-6
4. Slide the lower front cover to the left approximately 1/4-inch un-
til it clears the bezel, then lift up and out.
Top Cover Removal 2. Lift and remove the top cover by sliding it to the rear.
To remove: (See Figure 7-8).
Figure 7-8
Figure 7-7
Bezel Removal 4. Using a Phillips-head screwdriver, remove the outside bezel re-
taining screw. (See Figure 7-10).
A. CAUTION: Before starting this procedure, make sure
~ power to the instrument is turned OFF.
To remove:
1. Remove the upper front cover (page 7-5 for Model 1700 or
page 7-4 for Model 1700CS) and lower front cover (page 7-6
for Model 1700 or 7-5 for Model 1700CS)
2. Using a Phillips-head screwdriver, remove the inside bezel re-
taining screw. (See Figure 7-9).
Figure 7-10
Figure 7-9
3. Remove the frame cover on the lower right side of the instru-
ment (refer to Step 8 of the Main Power Supply Removal Pro-
cedure on page 7-53).
5. Remove the bezel by lifting it up and off. (See Figure 7-11). 7. Remove the cable connector connecting instrument to front
panel. If necessary, cut cable tie holding cable. (See Fig. 7-13).
Figure 7-11
6. Remove the ground wire. (See Figure 7-12).
Figure 7-13
Figure 7-12
Right Cover Removal 2. Slide the right side cover to the rear of the instrument about
To remove: 1f2-inch to clear the bezel hanger nuts and lift off.
(See Figure 7-15).
1. Using a Phillips-head screwdriver, remove the upper and lower
retaining screws holding the right side cover to the instrument
frame. (See Figure 7-14).
Figure 7-15
Figure 7-14
Left Cover Removal 2. Slide the left cover to the rear of the instrument about 1h-inch
To remove: to clear the bezel hanger nuts and lift off. (See Figure 7-17).
Figure 7-17
Figure 7-16
RBC and WBC Aperture Plate Removal 3. Pull the Aperture Plate straight out. (See Figure 7-19).
To prepare:
NOTE: Before attempting to remove the Aperture Plates, make sure
the instrument is ON.
Figure 7-19
Figure 7-18
NOTE: The cutout notch in the Aperture Plate must be facing down von Behrens WBC and RBClPLT Transducer Removal
and toward the instrument when the plate is reinserted into the
Both the von Behrens RBC and WBC transducers are removed accord-
transducer. Also note the "RP" etched in the Aperture Plate.
ing to the following procedure:
(See Figure 7-20).
To prepare:
1. Remove the upper front cover (page 7-5 for Model 1700 or
page 7-4 for Model 1700CS) and lower front cover (page 7-6
for Model 1700 or page 7-5 for Model 1700CS).
2. Remove the top cover (page 7-7).
3. Using a Phillips-head screwdriver, loosen the 2 screws holding
the aluminum shield at the top of the PAM (Pre-Amplifier Mod-
ule) and remove the shield. (See Figure 7-21).
Figure 7-20
4. Repeat the same procedure to remove the WBC Aperture
Plate. Note the location of the cutout notch and "WBC" etched
in the Aperture Plate.
After reassembly:
1. Verify appropriate gain adjustment (Section 8.13). After install-
ing the RBC/PLT Aperture Plate, verify RBC/PLT gains. After
installing the WBC Aperture Plate, verify WBC gain.
Figure 7-21
4. If any fluid remains in the transducers, make sure the instru-
ment is turned ON. From the MAIN MENU, press [SPECIAL
PROTOCOLS] then [MORE] followed by [DRAIN BATHS].
Turn the instrument OFF after the transducers have been
drained.
At... CAUTION: Make sure the power is turned OFF before 7. Label and disconnect all tubing from the von Behrens WBC
l.ll attempting to remove any electrical connection. Transducer. (See Rgure 7-23).
(.1
Figure 7-23
Figure 7-22
6. Snake wiring J4 through the Flow Panel.
8. Using a Phillips-head screwdriver, remove the 3 mounting 9. Lift the transducer assembly out of the instrument.
screws from the transducer holding plate. (See Figure 7-24). (See Figure 7-25).
Figure 7-26
11 . Snake the wiring J2 through the Flow Panel.
12. Label and disconnect all tubing from the von Behrens
RBC/PLT Transducer.
13. Using a Phillips-head screwdriver, remove the 3 mounting
screws from the transducer holding plate. (Refer to Step 9 of
the von Behrens WBC Transducer Removal procedure on
page 7-15).
14. Uft the transducer assembly out of the instrument. (Refer to
the von Behrens WBC Transducer Removal procedure on this
page).
WBC and RBC Metering Board Removal 5. Disconnect the tubing at the top and bottom of the metering
tube. (See Figure 7-28).
To remove the WBC Metering Board:
1. Remove the upper front cover (page 7-5 for Model 1700 or
page 7-4 for Model 1700CS) and lower front cover (page 7-6
for Model 1700 or page 7-5 for Model 1700CS).
2. Make sure the metering tube is drained by opening the vent so-
lenoid 2-1 located directly above the WBC Metering Board.
Figure 7-27
6. Using a 1/4 -inch nut driver, remove the 2 standoffs on the 10. Uft the board off the Flow Panel. (See Figure 7-30).
board. (See Figure 7-29).
V····
Figure 7-30
Figure 7-29
To remove the RBC Metering Board:
~ ATTENTION: OBSERVE PRECAUTIONS FOR HANDLING 1. Remove the upper front cover (page 7-5 for Model 1700 or
~ ELECTROSTATIC SENSITIVE DEVICES. page 7-4 for Model 170OCS) and lower front cover (page 7-6
for Model 1700 or page 7-5 for Model 1700CS).
7. Unplug the ground wire on the bottom standoff.
2. Make sure the metering tube is drained by opening the vent so-
lenoid (the horizontal solenoid directly above the RBC Meter-
IA. CAUTION: Make sure the power is turned OFF before ing Board).
ill attempting to remove any electrical connection.
IA. CAUTION: Before starting this procedure, make sure
8. Disconnect the red connector on the side of the metering
ill power to the instrument is turned OFF.
board.
9. Using a Phillips-head screwdriver, remove the 2 mounting 3. If any fluid remains in the metering tube before disassembly,
screws holding the metering board to the Flow Panel. drain it carefully using absorbent tissues.
4. Using a Phillips-head screwdriver, remove the 2 screws on the
metering board's transparent plastic shield. (Refer to Step 4 of
the WBC and RBC Metering Board Removal procedure on
page 7-17).
5. Disconnect the tUbing at the top and bottom of the metering NOTE: The RBC Metering Board is installed upside down. Push the
tube. (Refer to Step 5 of the WBC and RBC Metering Board metering tube all the way down to access the top screw hold-
Removal procedure on page 7-17). ing the metering board to the Flow Panel.
(See Figure 7-31).
CAUTION: When removing tubing, take extreme care not to break
the glass ends. The metering tube ends are fragile.
~
CAUTION: Take extreme care not to allow the liquid to
touch the printed circuit boards; it will short them out when
power is turned on. If board does get wet, make sure it is
dry before turning power back on.
Hemoglobin Flow Cell Removal 5. Swivel the top inner cover upward and to the rear. This cover
To prepare: is hinged and will stand in a folded back position without sup-
port. (See Figure 7-33).
1. From the MAIN MENU, press [SPECIAL PROTOCOLS] then
[MORE] followed by [DRAIN BATHS].
To remove:
1. Turn power to the instrument OFF.
2. Remove the top cover (page 7-7).
3. Remove the upper front cover (page 7-5 for Model 1700 or
page 7-4 for Model 1700CS) and lower front cover (page 7-6
for Model 1700 or page 7-5 for Model 1700CS).
4. Using a Phillips-head screwdriver, loosen the 2 holding screws
on the top inner cover. (See Figure 7-32).
Figure 7-33
6. Remove the aluminum shield at the top of the PAM (Pre-Ampli-
fier Module). (Refer to Step 3 in the von Behrens WBC and
RBC Transducer Removal procedure on page 7-13.)
Figure 7-32
8. Snake the HGB cable through the hole at the right side of the
...0... ATTENTION: OBSERVE PRECAUTIONS FOR HANDLING wac Metering Board and pull it through the hole.
~ ELECTROSTATIC SENSITIVE DEVICES. (See Figure 7-35).
7. Disconnect cable connector J1 (HGB cable) from the PAM.
(See Figure 7-34).
Figure 7-35
Figure 7-34
9. Remove the black tUbing from the T-connector above and be- 10. Using a Phillips-head screwdriver, remove the two retaining
low the HGB Flow Cell. (See Figure 7-36). screws holding the HGB Flow Cell to the Flow Panel.
(See Figure 7-37).
Figure 7-36
Figure 7-37
11. Snake the HGB cable behind the tubing installed at solenoid 2-
CAUTION: The tUbing is stiff. Use care when removing the tubing 6 and remove the HGB Flow Cell module.
to avoid breaking it off the HGB Flow Cell.
After reassembly:
1. After reassembly, perform HGB Zero Offset and HGB Gain Ad-
justment (Table 8-7 in Section 8.12).
2. Verify HGB precision.
Sample Probe Driver Assembly Removal CAunON: Make sure the power is turned OFF before
To prepare: attempting to remove any electrical connection.
1. Remove the upper front cover (page 7-5 for Model 1700 or
page 7-4 for Model 1700CS) and lower front cover (page 7-6 To remove:
for Model 1700 or page 7-5 for Model 1700CS).
2. Remove the top cover (page 7-7).
~
.a. ATTENTION: OBSERVE PRECAUTIONS FOR HANDLING
ELECTROSTATIC SENSITIVE DEVICES.
3. Using a Phillips-head screwdriver,loosen the 2 holding screws
on the top inner cover. (See Figure 7-38). 5. Mark and remove the cable connectors at the top of the cable
distribution board. There are four connectors that must be un-
plugged from this board: J20, J21, J22, and J23.
(See Figure 7-39).
Figure 7-38
4. Fold back the top inner cover (refer to Step 5 of the Hemoglo-
bin Flow Cell Removal procedure on page 7-20). Figure 7-39
6. Cut cable ties holding cables to other cables; there are ap-
proximately 8-10 cable ties.
7. Using a 3h2-inch Allen wrench, remove the two clamps holding
the Wash Block tubing and one clamp holding the aspiration
tubing.
8. Label and disconnect the 2 tubings from the Wash Block. 9. Disconnect the tubing from the top of the Sample Probe.
(See Figure 7-40). (See Figure 7-41).
12. Using a 7/64-inch Allen wrench, remove the 2 top and 2 bottom
~ ATTENTION: OBSERVE PRECAUTIONS FOR HANDLING mounting screws holding the Sample Probe Driver Assembly
~ ELECTROSTATIC SENSITIVE DEVICES.
to the chassis. (See Figure 7-43).
10. Disconnect the Sample Probe motor cable from the top chop-
per board. (See Figure 7-42).
Figure 7-43
13. Uft the Sample Probe Driver Assembly out of the instrument.
Figure 7-42
NOTE: Before lifting the Sample Probe Driver Assembly completely
11. Disconnect the Sample Probe motor cable from the bottom out of the instrument, check to make sure that all cables have
chopper board (located directly under the top chopper board). been disconnected. Disconnect any cables that you find still at-
tached, making sure to mark them before unplugging.
After reassembly:
1. Verify Sample Probe alignment (Section 8.15).
PAM (Pre-Amplifier Module) Removal 7. Flip the latch up and to the right. (See Figure 7-45).
To prepare:
1. Remove the upper front cover (page 7-5 for Model 1700 or
page 7-4 for Model 1700CS) and lower front cover (page 7-6
for Model 1700 or page 7-5 for Model 1700CS).
2. Remove the top cover (page 7-7).
3. Remove the bezel (page 7-8).
4. Remove the aluminum shield at the top of the PAM. (Refer to
Step 3 of the von Behrens WBC and RBC/PLT Transducer Re-
moval procedure on page page 7-13).
5. Using a Phillips-head screwdriver, remove the lower screw on
the bezel latch bracket attached to the chassis.
(See Figure 7-44).
Figure 7-45
Figure 7-44
6. Loosen but do not remove the top screw on the latch.
8. Using a Phillips-head screwdriver, remove the two left retain- CAUTION: Make sure the power is turned OFF before
ing screws on the left side of the PAM cover plate and loosen attempting to remove any electrical connection.
the two right retaining screws. (See Figure 7-46).
.a.
~
ATTENTION: OBSERVE PRECAUTIONS FOR HANDLING
ELECTROSTATIC SENSITIVE DEVICES.
10. Mark the 4 cables (J1, J2, J3, and J4) at the top of the PAM
board and disconnect them. (See Figure 7-47).
Figure 7-46
9. Slide the PAM cover to the left and off the PAM board.
Figure 7-47
11. Using a 1/4 -inch nut driver, remove the four metal standoffs on
the PAM board.
12. Lift the PAM board off the Flow Panel. (See Figure 7-48). Diluent Syringe Driver Assembly Removal
To remove:
1. Remove the top cover (page 7-7).
2. Loosen and told back the top inner cover (refer to Steps 4 and 5
of the Hemoglobin Flow Cell Removal procedure on page 7-20).
3. Remove the left side cover (page 7-11 ).
4. The fan above the Diluent Syringe Driver Assembly must be re-
moved before the driver assembly can be removed. To remove
the fan:
Figure 7-48 (a) Remove the 2 top fan screws. (See Figure 7-49).
After Reassembly:
Figure 7-49
(b) Loosen the 2 bottom fan bracket screws. (See Figure 7-50). 5. Disconnect the tubing on both sides of the directional valve at
the top of the Diluent Syringe. (See Figure 7-51).
8. Using a 7/64-inch Allen wrench, remove the 2 socket head 10. Lift the Diluent Syringe Driver Assembly out of the chassis.
screws on the front of the bottom holding plate of the driver as- (See Figure 7-53).
sembly. (See Figure 7-52).
Figure 7-53
Figure 7-52
After reassembly:
9. Using a 7/64-inch Allen wrench, remove the screw from the rear
base plate of the driver assembly. 1. Verify diluent volume (Section 8.14); verify calibration block
alignment (Section 8.16).
Sample Syringe Driver Assembly Removal 10. Lift the Sample Syringe Driver Assembly out of the chassis.
To remove: (See Figure 7-54).
4. Remove the fan located above the Diluent Syringe Driver As-
sembly (refer to Step 4 of the Diluent Syringe Driver Assembly
Removal procedure on page 7-28).
5. Disconnect the tUbing on top of the Sample Syringe.
Figure 7-54
6. Cut the cable ties holding the cable harness to the back of the
syringe assembly. After reassembly:
7. Disconnect ribbon cable #441 from the chopper driver board. 1. Verify sample volume (Section 8.14).
8. Using a 7/64-Allen wrench, remove the 2 socket head screws
on the bottom plate of the driver assembly (refer to Step 8 of
the Diluent Syringe Driver Assembly procedure on page 7-30).
9. Using a 7/64-Allen wrench, remove the screw from the rear
base plate of the driver assembly.
Figure 7-55
(b) Remove the tubes from the solenoids.
(c) Using a Phillips-head screwdriver, remove the 2 screws on 5. Disconnect the tubing from the top of the Lys$ Syringe.
the bottom of the inner bracket of the solenoid panel. (See Figure 7-57).
(See Figure 7-56).
Figure 7-57
Figure 7-56
6. Disconnect ribbon cable #446 from the choppier driver board.
(d) Pull the solenoid panel to the rear and set aside.
7. Using a 7/6 4-inch Allen wrench, remove the 2 socket head
screws on the bottom plate of the driver assembly (refer to
Step 8 of the Diluent Syringe Driver Assembly procedure on
page 7-30).
8. Using a 7/6 4-inch Allen wrench, remove the screw from the rear
base plate of the driver assembly.
9. Lift the Lyse Syringe Driver Assembly out of the chassis. Lyse Syringe Removal
(See Figure 7-58).
The procedure for removing the Lyse Syringe is covered in the
CELL-DYN 1700 Operations Manual. Refer to Section 9: Service and
Maintenance, Subsection: Lyse Syringe CleanlnglRemoval.
Figure 7-58
Fluid Power Supply Removal 4. Label and disconnect the tUbing from solenoid 5-5.
To remove: (See Figure 7-60).
Figure 7-60
Figure 7-59
5. Label and disconnect the tubing from the T-connector of the 6. Label and disconnect the tubing from solenoid 5-1.
0.5 psi supply line located behind the Flow Panel. (See Figure 7-62).
(See Figure 7-61).
Rgure7-62
Figure 7-61
8. Cut cable ties and disconnect the tUbing from the chassis floor
fitling next to the left pump assembly.
9. Disconnect the TYGON® tubing connected to the reagent
panel waste line (at the rear of the Reagent Inlet Panel).
10. Locate solenoid 1-6 and its corresponding sili¢one tubing. Fol-
low the tubing to the back of the Flow Panel, then label and dis-
connect the tubing at the in-line connector. Pull the line
through the hole to the back of the Flow Panel.
11. Remove the clamp and plug from the Vacuum Accumulator CAUTION: Make sure the power is turned OFF before
drain line, and pull the tubing through the rear of the Reagent attempting to remove any electrical connection.
Inlet Panel. (See Figure 7-63).
Rgure 7-63
Figure 7-64
17. Using an 18 inch or longer Phillips-head screwdriver, remove 18. Uft the Ruid Power Supply directly up and out of the instru-
the 3 corner mounting screws and the 1 central mounting ment. (See Figure 7-68).
screw holding the Fluid Power Supply to the base of the chas-
sis. (See Figure 7-67).
Figure 7-67
Figure 7-68
After reassembly:
1. Perform vacuum and pressure adj ustments (~ection 8.5) and
verify all count times (Section 8.6) .
MPM (Motor Processor Module) Board Removal Repeat this procedure for the snap-in pin at tHe center of the
To remove: board.
1. Remove the top cover (page 7-7). 5. Lift the MPM board off the top inner cover.
2. Loosen and fold back the top inner cover (refer to Steps 4 and 5 After reassembly:
of the Hemoglobin Flow Cell Removal procedure on page 7-20). 1. Perform the motor power test. (Refer to Table 5·3 and Figure 5-4
in Section 5).
A CAUTION: Make sure the power is turned OFF before
~ attempting to remove any electrical connection.
Figure 7-69
4. At each corner of the MPM board, compress the plastic snap-
in pins and pull the comer of the board free from the pin.
COM (Cable Distribution Module) Board Removal peat this procedure for the snap-in pin at the center of the
To remove: board.
1. Remove the top cover (page 7-7). 5. Lift the COM board off the top inner cover.
2. Loosen and fold back the top inner cover (refer to Steps 4 and 5
of the Hemoglobin Flow Cell Removal procedure on page 7-20).
~
.a. ATTENTION: OBSERVE PRECAUTIONS FOR HANDLING
ELECTROSTATIC SENSITIVE DEVICES.
Figure 7-70
4. At each corner of the COM board, compress the plastic snap-
in pin and pull the corner of the board free from the pin. Re-
CRT Assembly Removal CAUTION: Make sure the power is turned OFF before
To remove: attempting to remove any electrical connection.
Figure 7-72
Figure 7-71
5. Lift the PDM off the CRT chassis (cables still attached).
7. Disconnect the video connector to the SVGA board. DANGER: The CRT Assembly contains high voltage. Do
(See Figure 7·73). not touch the top of the CRT. Make sure y~ur ESD strap is
OFF.
Figure 7-73
CAUTION: Wear OSHA-approved safety glasses. Use extreme
care when removing the CRT to prevent possible CRT implosion
which could cause physical injury to exposed skin or eyes.
Figure 7-74
9. Lift the CRT Assembly out of the instrument. (See Figure 7-75). CPU Assembly Removal
To remove:
1. Remove the top cover (page 7-7).
2. Remove the bezel (page 7-8).
3. Remove the right side cover (page 7-10).
Figure 7-76
5. Disconnect the PC Power Supply cable (J4) from the Power 13. Slide the CPU Assembly out of the instrument.
Supply Module AC board (refer to Step 4 of the Main Power (See Figure 7-77).
Supply Removal procedure on page 7-52).
6. Disconnect the video connector to the SVGA board (refer to Step
7 of the CRT Assembly Removal procedure on Page 7-43).
7. Disconnect the standard PC Keyboard cable and any external
cables connected to the Graphics Printer or Ticket Printer Inter-
face boards.
8. Disconnect the 20-pin CCM communications cable from the
DLAboard.
9. Using a Phillips-head screwdriver, remove the 3 mounting
screws holding the frame cover to the lower right side of the in-
strument (refer to Step 8 of the Main Power Supply Removal
procedure on page 7-53).
10. Lift the frame cover off the instrument.
11. Using a Phillips-head screwdriver, remove the 4 mounting
screws on the lower right side of the instrument holding the
CPU Assembly to the chassis (refer to Step 10 of the Main
Power Supply Removal procedure on page 7-53).
12. Using a stubby Phillips-head screwdriver, remove the 3 front Figure 7-77
mounting screws holding the CPU Assembly to the chassis.
(refer to Step 11 of the Main Power Supply Removal proce-
dure on page 7-54).
PC Power Supply Removal 6. Remove the ground wire attached to the front of the disk drive
To remove: chassis. (See Figure 7-78).
A.
CAUTION: Make sure the power is turned OFF before
~ attempting to remove any electrical connection.
7. Using a Phillips-head screwdriver, remove the 4 mounting 9. Lift the PC Power Supply directly up and out of the CPU As-
screws, located on the right side of the instrument, holding the sembly. (See Figure 7-80).
PC Power Supply to the CPU Assembly. Make sure the power
~, :',··········,""
'.,
supply is supported while removing the screws. (See Figure 7-79). ..
.~*Yf:':·
I·················
Figure 7-80
Figure 7-79
8. Disconnect the PC Power Supply AC input.
I/O Boards Removal- CLA, SVGA, Ticket Printer, and Graphics CAUTION: Make sure the power is turned OFF before
PrinterlDisk Controller attempting to remove any electrical connection.
There are 4 boards plugged into the CPU Motherboard. As you face
the right side of the instrument, the 4 1/0 boards are located from right
to left as follows: To remove the DLA board:
1. The DLA (Data Link Adapter) board is located next to the Ana- 1. Remove the CPU Assembly (page 7-45).
log Card Cage.
2. The SVGA board is the second board from the card cage. ...0... ATTENTION: OBSERVE PRECAUTIONS FOR HANDLING
~ ELECTROSTATIC SENSITIVE DEVICES.
3. The Ticket Printer Interface board is the third board from the
card cage. 2. Disconnect the Membrane Keypad ribbon cable and the CCM
(Cell Count Module) communications ribbon cable from the
4. The Graphics Printer and Disk Drive Controller Interface board DLA board. (See Figure 7-81).
is the fourth board from the card cage.
Figure 7-81
3. Using a Phillips-head screwdriver, remove the bracket mount- CPU Motherboard Removal
ing screw holding the DLA board to the instrument chassis.
To remove:
(See Figure 7-82).
A CAUTION: Make sure the power is turn~d OFF before
~ attempting to remove any electrical connection.
Figure 7-82
4. Lift the DLA board directly up and out of the instrument.
To remove the SVGA, Ticket Printer, or Graphics PrinterlDisk Control-
ler board:
1. Remove the CRT Assembly (page 7-43).
5. Using a Phillips-head screwdriver, remove the 2 board mount- Disk Drive Assembly Removal
ing screws holding the CPU Motherboard to the CPU Assem-
bly. (See Figure 7-83). To remove:
5. Using a Phillips-head screwdriver, remove the remaining 3 Main (Linear) Power Supply Removal
mounting screws holding the Disk Drive Assembly to the instru-
ment chassis. (See Rgure 7-84). To remove:
Figure 7-84
6. Lift the Disk Drive Assembly up and out of the instrument.
7. Remove and replace the Floppy Disk Drive or Hard Disk Drive
from the Disk Drive Assembly as needed.
Figure 7-85
5. Label and disconnect connectors J1 and J2 on the PDM (refer to
Step 4 of the CPU Assembly Removal procedure on page 7-45).
6. Disconnect the video connector to the SVGA bo4rd (refer to Step
7 of the CRT Assembly Removal procedure on page 7-44).
7. Disconnect the standard PC Keyboard cable and any external 10. Using a Phillips-head screwdriver, remove the 14 mounting
cables connected to the Graphics Printer or Ticket Printer Inter- screws (2 are exposed by the removal of the frame cover) on
face boards. the lower right side of the instrument holding the CPU Assem-
bly to the chassis. (See Figure 7-87).
8. Using a Phillips-head screwdriver, remove the 3 mounting
screws holding the frame cover to the lower right side of the in-
strument. (See Figure 7-86).
Figure 7-87
Figure 7-86
9. Lift the frame cover off the instrument frame.
11. Using a stubby Phillips-head screwdriver, remove the 3 front 12. Slide the CPU Assembly forward and out of ttie instrument.
mounting screws holding the CPU Assembly to the chassis. (See Figure 7-89).
(See Figure 7-88).
Figure 7-89
Figure 7-88
13. Using a Phillips-head screwdriver, remove the 2 base mount- 14. Using a Phillips-head screwdriver, remove th~ 8 mounting
ing screws on the bottom of the Main Power Supply Assembly. screws on the back of the instrument holding fhe Main Power
(See Figure 7-90). Supply Assembly to the chassis. (See Figure 7-91).
15. Disconnect the DC cables J1 , J2, J4, and J5 on the Power 17. Pull the Main Power Supply Assembly out thJ back of the in-
Supply Module DC board. (See Figure 7-92). strument. (See Figure 7-93).
16. Pull the ribbon cable out of the snap holder at the bottom front
of the Main Power Supply.
Cage-Mounted Printed Circuit Boards Removal NOTE: When reinserting a board into its slot at the re~r of its cage,
Prior to removing any of the cage-mounted boards: press it firmly into place to ensure proper seati~g.
-
~I
"·1 .:
Figure 7-94
3. Pull the board out of the instrument.
Switching Power Supply Removal Needle Drive Assembly Removal- Closed Sample Module
To remove: To remove:
Figure 7-96
2. Remove the Interlock Cover.
4. Using a Phillips-head screwdriver, remove the 2 mounting 5. Using a Phillips-head screwdriver, remove th~ 2 mounting
screws under the tube guide. (See Figure 7-97). screws under the Closed Sample Module and! lift the cover off
the module. (See Figure 7-98).
Figure 7-97
Figure 7·98
6. Disconnect the silicone tubing from the top of the Needle Drive
Assembly.
7. Loosen the thumbscrew on the front of the Closed Sample
Module and swing the module outward.
8. Disconnect the sample line tUbing from the Peristaltic Pump 9. Using a Phillips-head screwdriver, remove the 2 mounting screws
tubing. (See Figure 7-99). on the chopper driver board cover plate. (See Figure 7-1 (0).
10. Disconnect the Needle Drive Assembly connector (J2) from 11. Using a Phillips-head screwdriver, remove the 4 screws hold-
the chopper driver board. (See Figure 7-101). ing the Needle Drive Assembly to the Closed Sample Module
frame. (See Figure 7-102).
Figure 7-101
Figure 7-102
12. Carefully pull the Needle Drive Assembly forward and out of Chopper Driver Board Removal- Closed Sample Module
the Closed Sample Module. (See Figure 7-103).
To remove:
1. Loosen the thumbscrew on the front of the Closed Sample
Module and swing the module outward.
2. Using a Phillips-head screwdriver, remove the 2 mounting
screws on the chopper driver board cover plate (refer to Step 9
of the Needle Drive Assembly Removal procedure, page 7-60).
~
..a... ATTENTION: OBSERVE PRECAUTIONS FOR HANDLING
ELECTROSTATIC SENSITIVE DEVICES.
Figure 7-103
Figure 7-104
5. Lift the chopper driver board off the Closed Sample Module.
Sample and Diluent Pumps Removal- Closed Sample Module 4. Disconnect the 3 silicone tubings from the Sample Cup.
To remove the Sample Peristaltic Pump: (See Figure 7-106).
Figure 7-106
Figure 7-105
5. Using a Phillips-head screwdriver, remove the 3 remaining cor- 7. Disconnect the motor cable from the chopper driver board corre-
ner mounting screws and 1 central mounting screw on the peri- sponding to the Sample Peristaltic Pump. (See Rgure 7-108).
staltic pump support plate. (See Figure 7-107).
Figure 7-108
Figure 7-107
8. Pull the Sample Peristaltic Pump off the Closed Sample Module.
6. Carefully pull the support plate forward to gain access to the
chopper driver boards. To remove the Diluent Peristaltic Pump:
1. Loosen the thumbscrew on the front of Closed Sample Module
and swing the module outward.
2. Remove the tubing from behind the Diluent Peristaltic Pump.
3. Using a Phillips-head screwdriver, remove the 2 mounting
screws holding the Diluent Peristaltic Pump housing to the
Closed Sample Assembly (do not remove the upper right
screw on the sample pump housing). Refer to Step 3 of the
Sample Peristaltic Pump Removal procedure, page 7-63.
4. Disconnect the 3 silicone tubings from the Sample Cup (refer
to Step 4 of the Sample Peristaltic Pump Removal procedure,
page 7-63).
5. Using a Phillips-head screwdriver, remove the 3 remaining cor- Fan Air Filter Removal
ner mounting screws and 1 central mounting screw on the Peri-
To remove:
staltic Pump support plate (refer to Step 5 of the Sample
Peristaltic Pump Removal procedure, page 7-64). 1. Grasp the plastic air filter holder at one of the corners and
snap off. No tools are necessary.
6. Carefully pull the support plate forward to gain access to the
chopper driver boards. 2. Remove the old filter from the holder and replace it with a new
filter.
7. Disconnect the motor cable from the chopper driver board cor-
responding to the Diluent Peristaltic Pump. 3. Snap the plastic holder back into place.
8. Pull the Diluent Peristaltic Pump off the Closed Sample Mod-
ule.
After reassembly:
1. Perform the motor power test (refer to Table 5-3 and Figure 5-
4 in Section 5).
2. Verify CS precision. Verify open/closed operation.
Table of Contents
8.1 SECTION OVERVIEW
8.2 TEST EQUIPMENT AND SUPPLIES REQUIRED
8.3 PREPARATION FOR ALIGNMENTNERIFICATION
8.4 ORDER OF ALIGNMENTNERIFICATION
8.5 VACUUM AND PRESSURE ADJUSTMENTS
Regulator Alignment
Pressure Adjustment (0.5 psi)
Pressure Verification (High)
Vacuum Adjustment (8 inch)
8.6 METERING SYSTEM TIMING ADJUSTMENTS - RBC AND WBC
RBC Metering System Timing Adjustment
WBC Metering System Timing Adjustment
8.7 24V SWITCHING MODULE ADJUSTMENT-
8.8 POWER DISTRIBUTION MODULE TEST POINTS
8.9 CABLE DISTRIBUTION MODULE TEST POINTS
8.10 SIGNAL PROCESSOR MODULE ADJUSTMENT
List of Figures
Figure 8-1 CELL-OYN 1700 Metering Timing Chart
Figure 8-2 RBC Metering Tube
Figure 8-3 WBC Metering Tube
Figure 8-4 PDM Test Points
Figure 8-5 SPM Test Points
Figure 8-6 OCM Test Points
Figure 8-7 PAM Test Points
Figure 8-8 MAM Test Points
Figure 8-9 WBC Gain Adjustments
Figure 8-10 RBC Gain Adjustments
This chapter discusses the alignment and verification procedures for the CELL-DYN 1700. These
procedures are used to ensure the proper electronic alignment of the circuitry.
These procedures also serve as a method of isolating a defective assembly, module, or printed circuit
board.
Service representatives must ensure that all external components of the system, such as reagents,
blood samples used, controls and calibrators, environment, and AC power, are acceptable and cor-
rect before proceeding with the alignment and verification procedures.
Perform the following procedure to prepare the CELL-DYN 1700 for alignment/verification:
1. Verify all reagents are correct and available in sufficient quantities to perform 100-150
cycles on the instrument.
2. Remove the upper and lower front covers, left and right side covers, and top cover.
3. Remove and clean both RBC/PLT and WBC aperture plates following the procedure in
Section 9: Service and Maintenance, Subsection: Aperture Plates Cleaning of the
CELL-DYN 1700 Operations Manual.
4. Clean the HGB Flow Cell following the procedure in Section 9, Subsection: HGB Flow
Cell Manual Cleaning of the CELL-DYN 1700 Operations Manual.
5. Re-initialize the instrument by turning the system OFF then ON again. When
Initialization is complete, press [PRIME/RUN] to prime the instrument. Observe the flow
system for leaks, tubing placement, pinched tubing, etc.
6. Reinstall the covers on the instrument.
7. Run a Background count. Verify all background values are within the following
specifications:
• WBC < 0.5 KlflL
• RBC < 0.05 M/flL
• HGB < O. 1 g/dL
• PLT < 10.0 KlflL
8. Enter the date and time according to directions in Section 2: Installation Procedures
The following procedures are presented to ensure proper alignment of the CELL-DYN 1700.
1. Vacuum and Pressure Adjustment (Section 8.5)
2. RBC Count Time Adjustment (Section 8.6)
3. WBC Count Time Adjustment (Section 8.6)
4. 24V Switching Module Adjustment (Section 8.7)
5. Power Supply Voltage Checks and Adjustment (Section 8.8)
6. Signal Processor Module Alignment (Section 8.10)
7. Device Control Module Alignment (Section 8.11)
8. Pre-Amplifier Module Alignment (Section 8.12)
9. Main Amplifier Module Alignment (Section 8.13)
The CELL-DYN 1700 utilizes one vacuum and two pressure levels to accomplish the following tasks:
moving sample, reagents, and waste, bubble mixing of sample, and backflushing RBC and WBC
apertures. The vacuum and bubble mix pressures are adjustable by a solid-state regulator. The
backflush pressure is not critical and the pump is under direct computer control.
The solid state regulator has two input ports: P1 for pressure and P2 for vacuum. It also has jumper
terminals which accommodate all desired vacuum and pressure ranges.
Regulator Alignment
This offset adjustment must be performed with no pressure or vacuum applied to the regulator. Fol-
low the steps below:
1. Disconnect the appropriate pumps on the pump relay module and bleed off pressure
from the accumulators.
• J4 for low pressure
• J2 for vacuum on new-style pumps
2. Remove the pressure or vacuum line from the top of the regulator.
3. On the Pump Relay Board, the voltage should be 5.0 +/- 0.15 volts at J7 pin 7 for
vacuum, and J8 pin 5 for pressure.
NOTE: You must remove the board mounting screws and free the rest of the board to make the re-
maining adjustments. Disconnect J6 or J7 while relocating the appropriate printed circuit board
to avoid shorting out components.
4. Note the current position of jumper E1 and set E1 to the C-D position.
The instrument uses the Volumetric Metering process to regulate the count cycle and to ensure that a
precise volume of sample is analyzed for the measurement. Table 8-1 shows the results of a fault
report displayed on the screen if a flow error or clog occurs during a Run cycle. Figure 8-1 illustrates
the timing relationships for WBC and RBC measurements.
For each transducer there are two distinct counting periods, T1 and T2. Figures 8-2 and 8-3 illustrate
the counting periods for the RBC and WBC metering tubes, respectively.
WBC
UPPER
0 1.0 3.0
LOWER
0 4.0 6.5
RBC/PLT
UPPER
0 4.0 6.0
LOWER
Figure 8-1 CELL-DYN 1700 Metering Timing Chart (In Seconds) SS00034A
RBCVALVE VENT
-
-
,........
(
~
TO TRANSDUCER I
'--
I~ T1
1m
~
D D UPPER DETECTOR
RBe
T2
~D D LOWER DETECTOR
WBCVALVE VENT
-
.....
{
r--
TO TRANSDUCER I
'-- 0
T1
g
~
~
D D UPPER DETECTOR
WBC
T2
~D D LOWER DETECTOR
The 24V Switching Module is located next to the fan behind the CRT assembly.
Adjust the regulated +24V supply to 24.5 +/- 0.5V.
Location Function Voltage.
BROWN +24V 25.0V +/- 1.0
NOTE: The 24V Switching Module has only one potentiometer. Adjust the potentiometer with an in-
sulated screwdriver and record the adjustment.
Table 8-2 lists the test points located on the Power Distribution Module (PDM) and Figure 8-4 illus-
trates the test points on the PDM board.
• • •
TP4 TP3 TP2
PDM
TP1
• •
TP5 GND
Table 8-3 lists the test points located on the Cable Distribution Module (COM).
The Signal Processor Module (SPM), located in the card cage on the right side of the instrument, con-
tains the circuitry for the RBC and WBC lower fixed discriminators and the lower and upper platelet
discriminators. A discussion of the functions of the SPM can be found in Section 4 of this manual;
however, the only field adjustments recommended on this board are the discriminator voltages.
Follow the steps below to align the SPM:
1. Verify that the instrument is in the READY state.
• •
TP3 TP4
J1
0 • • • 0 0 00 •
TP9
TP5
TP1 TP2 SPM
•
TP8
R39 R41 R42 TP19
•
TP18
•• • • TP6
R56
TP7
R40
TP20
• •
TP15
•
TP10 TP11
• 0 0 TP16
TP13
O· •
TP12
• 0 TP14
• R44 R45
TP17
R48 R49
The Device Control Module (DCM) is located in the main electronics card cage. The DCM has a sin-
gle adjustment that can be performed in the field. The adjustment is for the 0 to A converter output.
No other adjustments are required.
Follow the steps below to align the DCM:
1. Verify the instrument is in the READY state.
2. Connect the DVM positive lead to TP3 on the DCM. Connect the ground lead to TP2
(DAC GND) on the DCM board.
3. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times. Press
[SERVICE DEC CODE], type "2" and press Enter.
4. Adjust R1 for 9.0 volts +/- 0.07 volts.
5. Press [SERVICE DEC CODE], type "1" and press Enter.
6. Check TP3 for 4.5 volts +/- 0.07 volt.
NOTE: Counterclockwise rotation increases the voltage.
J1 • 11..-- J_2 _
TP1
•
TP2
R1
oeM
• TP3
The Pre-Amplifier Module (PAM) is located on the upper right corner of the Flow Panel. Hemoglobin
circuitry, and the PLT and WBC aperture currents require verification and/or adjustment on this mod-
ule. The HGB Flow Cell should be cleaned before performing hemoglobin alignments (refer to Sec-
tion 9, Subsection: HGB Flow Cell Manual Cleaning in the CELL-DYN 1700 Operations Manual).
J1
II J2
I I J3
I J4
I
•
• TP6 +15VDC
TP7 -15V DC
• • •
TP2 TP3 TP4
ANALOG
GROUND
•
TP5
TP8. • TP9
R5
(] R12 K2 K3
(] • TP1 +100V DC
R13
I~I I~l
(] G R21
PAM
R35
The Main Amplifier Module (MAM) is located in the main electronics card cage. Alignment of the
WBC, RBC, and PLT gains are critical adjustments that must be verified and/or adjusted before instru-
ment accuracy can be established.
Uniform Latex particles are used to perform these adjustments. The particles must be mixed vigor-
ously before diluting to obtain accurate results.
The Gain and RBC Cell Editing adjustments are performed in the Gain Adjust Mode, which allows
multiple counts to be run on the same sample. When Gain Adjust Mode is entered, whatever is in the
Pre-Mixing Cup is transferred to the WBC Cup and 5 mL of diluent is left undisturbed in the RBC Cup.
WBC Offset
1. Ensure that the instrument is in the READY state.
2. Connect the DVM Pos lead to TP1 O. Connect the ground to TP9.
3. Adjust R1 (WBC ZERO ADJUST) so that the baseline of the noise signal is positioned at
0.000 volts +/- 0.002.
WBC Gain
1. Prepare a WBC latex dilution.
a. Obtain a clean container. From the MAIN Menu, press [SPECIAL PROTOCOLS] fol-
lowed by [MORE] twice to display the [10 mL DISPENSE] key. Press [10 mL DIS-
PENSE] four times to dispense 20 mL of diluent into the container (must press twice to
dispense 10 mL).
b. Add 1 drop of well mixed 5.0 latex particle solution into the 20 mL of diluent and mix
well.
c. Before entering the Gain Adjust mode, use the WBC latex dilution prepared in step
(b) above to fill the Pre-Mixing Cup up to the level of the diluent inlet port.
R1 R4 R5 R6 R7 R8 R9 R11 R71
.0 0 OOOT;.OO 0 00. • 0 •
R15 R16
TP12 TP13
TP10 TP6 TP7 •
TP11
• TP2 R72
•
TP14
• TP1 MAM •
TP15 0
• TP3 • TP4 • TP5
.TP8
• TP16
NOTE: When the procedure is completed, or if there is a need to exit the Gain Adjust Mode while per-
forming this procedure, such as constant clogs or improper dilution ratio, press [SPECIMEN
TYPE] followed by [PATIENT SPECIMEN].
RBC Offset
1. Connect a DVM to TP6 (RBC OUT) and the ground to TP9.
2. Adjust R11 (RBC ZERO ADJUST) so that the baseline of the noise signal is positioned
at 0.00 volts +/- 0.002 volts.
3. Connect a DVM to TP13 (RBC DISCRIMINATOR) and the ground to TP9.
4. Adjust R71 (RBC DISC. ADJUST) for 0.40 volts +/- 0.01 volts.
CELL-DYN® 1700 Service Manual 9140265A-February1995 8-36
Section 8 ALIGNMENT AND VERIFICATION
CELL-DYN 1700 SPECIMEN DATA REPORT
Dec 121993
Type: GAIN ADJ Operator I.D.:
I
Approximately 95 II
Sequence# :
wec
wac: 18.2 GIL
LYM: 13.7 R2 75.3 %L
*MID: 4.0 RM 21.8 %M
GRAN: 0.5 R3 2.9 %G 100 200 300 400 450
CD1700 DIAGNOSTIC REPORT
CD 1700 Serial #: Dec 17 1994 11 :38 am
UIS: Version 1.0 Operator 10: -••
Sequence # : 25
wac histogram data smoothing
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
1: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
17: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
33: 0 0 0 0 0 0 1 1 1 1 2 4 6 9 17 27
49: 40 52 65 76 87 95 99 100 98 96 94 91 87 83 79 74
65: 69 64 58 54 49 46 41 38 35 33 32 30 29 30 30 30
81 : 29 28 26 23 20 16 13 9 7 5 3 3 2 2 2 2
97: 2 2 1 1 1 1 1 2 2 2 2 1 2 2 2 2
113: 2 2 2 2 2 2 2 2 2 2 1 1 2 1 1 1
129: 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
145: 1 1 0 0 0 0 0 1 1 1 1 1 1 1 1 0
161 : 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
177: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
193: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
209: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
225: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
241: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peak of 100 is at channel 56
'--
'-- 5.0 LATEX APERTURE SIZE - 60 MICRON OIA.
f.----
33
I ~ ....~""
~
~
~
32 I~
~
.~
.........
..... ~
31 ~
1.01III"'"
~
~
-
~
30
~
~
...... II""'"
--
0 ~
~ 29
~
.............. II""'"
I- ~
~
Cl
w 28 ...
..... II""'"
~
IiIll"""
1.01III"'"
l..llllII""
27
.,
~
~
~
II
26 ~
... ~
LI
25 I--
I---
I---
RBC COUNT IN MILLIONS PER MICROLITER
I I
PLT Offset
1. Connect a DVM to TP7 (PLT OUT) and ground to TP9.
2. Adjust R16 (PLT ZERO ADJUST) so that the baseline of the noise signal is positioned at
0.00 volts +/- 0.002 volts.
PLT Gain
1. If in the Gain Adjust mode, prepare a PLT latex dilution by following the steps below:
a. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice.
Place a clean container under the Sample Probe and press [10 mL DISPENSE] four
times to dispense 20 mL of diluent (must press twice to dispense 10 mL).
b. Add one (1) drop of well-mixed 3.31 latex particle solution and mix well.
c. Hold the diluted latex solution under the Sample Probe and press [1/50 DILUTION] to
dispense a second dilution.
d. Place a clean container under the Sample Probe and press [1/50 DILUTION] to
aspirate the second dilution.
e. Pull open solenoid 2-5 to drain the existing solution in the RBC Mixing Chamber.
f. Pour the second latex solution into RBC Mixing Chamber.
To minimize problems like coincidence passage, the CELL-DYN 1700 uses two different dilution
ratios of whole blood to diluent. The ratio for WBC/HGB is 1:285; the ratio for RBC/MCV/PLT is
1:12,801. The following procedure will be used to verify the diluent dispense to maintain proper dilu-
tion ratios and thereby optimize instrument performance.
2 5 10 15 20 25 30 35
CD1700 DIAGNOSTIC REPORT
The following procedures provide step-by-step instructions to correctly adjust the positions of
Microswitches 1 through 4 on the probe assembly and to correctly align the Sample Probe height.
If the diluent syringe needs to be replaced, the calibration block must be removed from the old syringe
and placed on the new syringe. Follow the instructions below for removing and installing the calibra-
tion block on the syringe. Figure 8-14 illustrates the diluent syringe.
NOTE: Refer to Section 9, Subsection: Diluent Syringe Cleaning in the CELL-DYN 1700 Opera-
tions Manual for detailed instructions on removing and replacing the diluent syringe.
1. Remove the diluent syringe from the instrument as described in the CELL-DYN 1700
Operations Manual.
2. Remove the calibration block from the old syringe using a 7/64" Allen wrench.
3. Install the new syringe in its luer lock fitting.
4. Slide the calibration block onto the plunger rod of the new syringe, then press [SYRINGE
UP] to move the drive ring up. The calibration block should be resting on top of the ring.
5. Reinstall the front section of the syringe holding clamp. Secure it with the clamp nuts
removed during the syringe removal procedure (refer to step 1). Install the clamp nuts
with the larger hole facing the screw. Tighten the clamp nuts finger-tight with the
beveled edge toward the holding clamp. Do not overtighten.
o
::0
<
m
z
c
en
en
8
lG
~
Perform the following procedure to adjust the Short Sample Sensor on the Closed Sample Assembly:
1. Obtain a new vial of CELL-DYN 16 Low Control.
2. Using CELL-DYN diluent, dilute the Low Control to obtain a reading of 0.8 MIL +1- 0.08
for RBC on the Final Function Test reference instrument.
NOTE: Always use CELL-DYN diluent for sample dilution and always use the diluted solution the same
day. Do not allow the diluted solution to sit overnight.
3. Hook the positive probe of the DVM to TP4 and the negative probe of the DVM to TP3
(ground of the Blood Sensor printed circuit board #9601165 located inside the Closed
Sample Assembly).
4. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times then
[SERVICE DEC CODE]. The message <ENTER NUMBER (CURRENTLY, XXX):> will
be displayed on the screen.
5. Place a well-mixed VACUTAINER® of sample in the tube holder well (cap facing down)
and snap the tube in place. Type "33" and press Enter.
6. When the flow sequence is completed, adjust R1 and set the voltage of TP4 to 2.5V +1-
0.1V.
7. Measure the voltage of TP1.
8. If the voltage is within the range of 0.3V - 3.25V, press [SERVICE DEC CODE] again.
The message <ENTER NUMBER (CURRENTLY, 33):> will be displayed on the screen.
PLANNED MAINTENANCE
SECTION OVERVIEW
The Planned Maintenance Checklist for the CELL-DYN 1700 is available to the Field Service Repre-
sentative on the lap-top computer assigned to the representative and is also available from the Tech-
nical Services Group.
Future revisions of the Planned Maintenance Checklist will be released as Technical Service Bulle-
tins.
Table of Contents
B.1 SECTION OVERVIEW
B.2 CLOSED SAMPLE DESCRIPTION
Detection of Closed Sample Capability
Detection of Short Sample (Insufficient Aspiration)
Setting Open, Closed, and Pre-Dilute Calibration Factors
Using Count Test Function in the Closed Mode
Auto Clean Function
Clean Sampler Function
B.3 CLOSED SAMPLE MODULE CONFIGURATION
B.4 CLOSED SAMPLE ASSEMBLY FUNCTIONAL SEQUENCE DESCRIPTION
B.5 CLOSED SAMPLE MODULE TROUBLESHOOTING
Service DEC Codes
Motor Power Test (Service DEC Code 128)
Motor Exercise Test (Service DEC Code 130)
List of Figures
Figure 1 Ready Mode
Figure 2 VACUTAINER@ Vent
CELL-DYN® 1700 Service Manual 9140265A-February1995 B-1
~endix B _ _ CLOSED SAMPLE OPTION
Figure 3 VACUTAINER~ Aspiration :...----------------
Figure 4 Continued VACUTAINER@ Aspiration
Figure 5 VACUTAINER@ Aspiration Completed
Figure 6 Sample Presentation
Figure 7 Sample Drain
Figure 8 Sample Transfer Cup Soak
Figure 9 Sample Transfer Cup Rinse
Figure 10 Sample Transfer Cup Rinse Complete
Figure 11 System Rinse
Figure 12 Diluent Tubing Air Gap
Figure 13 System Rinse Completed
This appendix is designed to aid the Field Service Representative (FSR) in the troubleshooting and
repair of the CEll-DYN 1700CS Closed Sample assembly. Before attempting any repair of the
Closed Sample assembly, the FSR should verify that the instrument is operating properly in the Open
mode and that the problem is being caused by a malfunction in the Closed Sample assembly.
When a closed VACUTAINER® is inserted (cap facing down) into the VACUTAINER® holder well
and the Touch Plate on the Closed Sample Assembly is pressed, the instrument aspirates 450 Jll of
sample. The Closed Sample cycle performs the following four major functions:
1. Diluent residue rinse (from the previous cycle)
2. Sample presentation and aspiration
3. Sample transfer cup soak and rinse
4. Needle waste well rinse
A detailed description of the steps in each of these functions is given in Section B.4.
The Closed Sample Module includes the following major assemblies. Refer to schematic #9480081 in
Section 6.
1. Needle Drive Assembly, Needle Drive Motor, and Stepper Driver printed circuit board
2. Sample Pump Motor and Stepper Driver printed circuit board
3. Diluent Pump Motor and Stepper Driver printed circuit board
4. Sample Transfer Cup
5. Sample Detector
The following is a description of the functions performed by the Closed Sample Assembly during a
sample cycle. The sequence begins when the instrument is in the READY mode and the Touch Plate
is pressed. The sequence ends when the instrument returns to the READY state.
Legend:
The normal solid line for the tubing indicates the Closed Sample Assembly is in the READY state.
The heavy solid line for the tubing indicates the line is filled with liquid during the closed sample cycle.
The dashed line for the tubing indicates the line contains residue during the closed sample cycle.
Needle Waste
Down
Waste
a o
Sample Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 1 Ready Mode
SS00037A
T
Probe
Needle Waste
in Vent
Position Waste
..... .....---+-----..
~ Diluent
a o
Sample Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 2 Vacutainer Vent
SS00038A
Needle in Waste
Aspiration
Position Waste
....--tl-----+----~Diluent
Sample Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 3 Vacutainer Aspiration
SS00039A
Waste
Waste
Sample
o
Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 4 Continued Vacutainer Aspiration
SS00040A
~
I
I
I
I
- - - -- - -- -J
Waste
Waste
..... .....----+----.......
~ Diluent
Sample
o
Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 5 Vacutainer Aspiration Completed
SS00041A
~
I
I
I I
I I
Needle
Down - - - - - - - Waste
Waste
- -- -J
~
_r'"
0
Sample Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 6 Sample Presentation
SS00042A
I
I
I I
- - - - - - - Waste
Waste
- - -
I .....- ......--.....p.---......... Diluent
~
_r'
0
Sample Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 7 Sample Drain
SS00043A
Vacutainer
Sample Transfer Cup i
28 ~
1
1
1 1
1
Needle
Down - - - - -- - 1 Waste
Waste
- -- _I
0
_r-
~
Sample Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 8 Sample Transfer Cup Soak
SS00044A
T
Probe
I
I
Needle I Waste
Down
Waste
o
_r
Sample Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 9 Sample Transfer Cup Rinse
SS00045A
T Probe
I I~BIOOd
I Residue
I I
- -1-
Waste
Waste
- - -- _I
Blood ~
Residue/" .. .....- .... ----l~---~ Diluent
O~
--
Sample Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 10 Sample Transfer Cup Rinse Completed
SS00046A
T
Probe
Waste Waste
Well
Waste
....~t------+----.......~ Diluent
Sample Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 11 System Rinse
SS00047A
Vacutainer
T
Probe
~...."Sample Transfer Cup
~Air Gap
Short
Sample
Detector
Waste
Waste
....-+----+0----..... Diluent
~ [])
Sample Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 12 Diluent Tubing Air Gap
SS00048A
T
Probe
Waste
Waste
.--....--_1----- Diluent
Sample Diluent
Peristaltic Peristaltic
Pump Pump
Piercing
Stepper
Figure 13 System Rinse Completed
SS00049A
o
L/12 Diluent 1
o
The CELL-DYN 1700 Instrument System Interface Specification is contained in this section. It is a
separate document published by Abbot Diagnostics. It is included in this manual to assist Field Ser-
vice Representatives in answering questions and resolving problems related to the interface of the
system to an external computer.
SYSTEM INTERFACE SPECIFICATION
This document describes the interfacing characteristics of the Abbott CELL-DYN® 1700
automated hematology analyzer when attached to a Host.
The CELL-DYN 1700 provides a standard D8-9 male connector, labeled COM 1,
mounted on the side of the instrument.
Pins on the D8-9 connector: (standard RS-232 9 pin assignments)
Pin 1: Data Carrier Detect (DCD input) (monitored)
Pin 2: Data to CELL-DYN 1700 (RD - receive data) (ACKINAK, XNO/XOFF)
Pin 3: Data to Host (LIS, results) (TD - transmit data)
Pin 4: Data Terminal Ready (DTR) (set true)
Pin 5: Signal Ground
Pin 6: Data Set Ready (DSR) (monitored)
Pin 7: Request to Send (RTS output) (set true)
Pin 8: Clear to Send (CTS input) (monitored, if requested in Setup)
Pin 9: Ring Indicate (RI input) (unused)
3.2 The maximum recommended cable length is 30 meters, or 100 feet. The actual maxi-
mum workable cable length is dependent on the environment of the site, the selected
baud rate, and the equipment being connected together.
4.2 All information transmitted is in character form and is represented by 7-bit ASCII.
4.3 Transmitted characters consist of one (1) start bit, seven (7) or eight (8) data bits (Ieas~
significant first), one or no parity bit, and one (1) or two (2) stop bits.
4.5 The transmission speed may be selected from 1200, 2400, 4800, or 9600 bits per sec-
ond (bps).
Transmission control is provided in two ways: 1) XOFF/XON protocol in which the Host
transmits an XOFF character (hex 13) to stop transmission from the System and an
XON character (hex 11) to re-start transmission; and 2) CTS (Clear To Send) hardware
control.
The XOFF/XON protocol has a 1.5 second time-out. If no XON is received for 1.5 sec-
onds after an XOFF, then transmission resumes anyway.
The CTS hardware control has no time-out. That is, no data transmission will be started
with CTS false. However, the transmission will still time-out if at anyone time CTS
remains false for longer than the time-out selected in the COMPUTER SETUP Menu
(see Section 5.3.1 below). This is a different use and meaning for time-out, but it is
needed to prevent the Host from indefinitely delaying the CELL-DYN 1700 from pro-
cessing the next sample. Re-transmission requests are also supported and discussed
in Section 5.3.
Numeric data are transmitted in fields of fixed length with zeros used to fill empty
spaces on the left. Out-of-range numeric values are represented by strings of ">"
characters (hex 3E), and undefined numeric values are represented by strings of "-"
characters (hex 20). Alphanumeric data are transmitted in fields of fixed length
enclosed in double quotation marks (hex 22). Within the quotation marks, the data
are right-justified and blanks (hex 20) are used to fill empty spaces. Fields are sepa-
rated by commas (hex 2C).
6.1 The Identification segment of each message identifies the type of message and the
specimen the message represents.
The Message Type field identifies the message. There are four message types:
WBC Histogram Message - Type "WBC"
RBC Histogram Message- Type "RBC"
PLT Histogram Message - Type "PLT"
Count Data Message - Type"" (3 blanks)
The Instrument Type is an alphanumeric field of seven (7) characters enclosed in dou-
ble quotation marks. The string for the CELL-DYN 1700CS is sent as "CD1700C" and
for the CELL-DYN 1700 as " CD 1700".
The Sequence Number is a numeric field of four (4) characters with a value ranging from
o to 4999.
6.6 Specimen Type - Field 5
The Specimen Type is the fifth field in the Identification Segment. It occupies a numeric
field of two characters whose value identifies the specimen type as follows:
00: Patient type "PATIENT"
01: Replicate 1 "REPLIC 1"
02: Replicate 2 "REPLIC 2"
03: Replicate 3 "REPLIC 3"
The Operator ID is a alphanumeric field of three (3) digits enclosed in double quotation
marks. If no Operator ID is specified, the field is transmitted as "---".
The Specimen Date, giving the date on which the specimen was run, is an alphanumeric
field of eight (8) characters enclosed in double quotation marks. The default format of
the date is MM/DDIYY, where MM represents the month in two digits, DD represents the
day of the month, and YY represents the year. The date format can be changed in the
DATE/TIME Menu.
The Specimen Time is a alphanumeric field of five (5) characters enclosed in double
quotation marks. It gives the time at which the specimen was run in standard 24-hour
format.
The Specimen Name is the tenth field in the Identification Segment. It occupies a text
field of sixteen (16) characters enclosed in double quotation marks, and it is undefined
except for patient samples.
The Specimen Sex field consists of one (1) character "M" or "F" enclosed in double quo-
tation marks. If not entered by the operator, Specimen Sex is transmitted as a blank" ".
The Specimen Date of Birth field has an identical format to Field 7, except that Field 7 is
automatically generated by the CELL-DYN 1700. If DOB is not entered by the operator,
it is transmitted as "bb/bb/bb" where the "bb"s are actually 2 ASCII blanks each.
The Dr Name field is an alphanumeric field of twenty two (22) characters enclosed in
double quotation marks.
The Collection Date field is an alphanumeric field of five (5) characters enclosed in dou-
ble quotation marks. The format is MM/DD, where MM represents the month in two dig-
its and DO represents the day. The year is not included. It gives the date at which the
specimen was collected. If the Collection Date is not entered, it is transmitted as "bb/bb"
where the "bb"s are 2 ASCII blanks as in Field 12 above.
The Collection Time field is an alphanumeric field of five (5) characters enclosed in dou-
ble quotation marks. It gives the time at which the specimen was collected. If the Collec-
tion Time is not entered, it is transmitted as "bb:bb" ("bb"s are 2 ASCII blanks each and
":" is the middle byte).
The Comment field is an alphanumeric field of sixteen (16) characters enclosed in dou-
ble quotation marks.
Refer to Table 3, Count Data Messages, and Table 4, Count Record Dump.
The parameters reported by the instrument may be represented in any of four different
sets of measurement units as follows:
Set 1 - Standard USA
Set 2 - SI
Set 3 - Modified SI (HGB/MCHC in mmol/L, MCH in fmol)
Set 4 - Modified SI (HCT/PCT in %)
NOTE: The numeric values transmitted to the host are always sent in Standard USA
units even if the unit of measure, also being transmitted, is Set 2, 3, or 4.
-"--"---- - -'-"- -
" "-"
7.3.33 LRI Flag - Field 61
The LRI Flag is in a numeric field of two characters. A value of 16 indicates that the
flag is set, and a value of 00 indicates that it is clear.
7.3.34 URI Flag - Field 62
The URI Flag is in a numeric field of two characters. A value of 32 indicates that the
flag is set, and a value of 00 indicates that it is clear.
2C 30 35 32 2C 30 35 33-2C 30 35 31 2C 30 35 30 ,052,053,051,050
20 20 20 20 20 20 20 20-20 20 20 20 20 31 22 2C 1",
22 40 22 2C 22 20 20 2F-20 20 2F 20 20 22 2C 22 "M" ," / / " , "
20 20 20 20 20 20 20 20-20 20 20 20 20 20 20 20
20 20 20 20 20 20 22 2C-22 20 20 2F 20 20 22 2C " , "/" ,
22 20 20 3A 20 20 22 2C-22 20 20 20 20 20 20 20 " . ""
,
20 20 20 20 20 20 20 20-20 22 2C 30 30 35 32 35 ",00525
2C 20 20 20 20 20 2C 30-30 30 31 30 2C 30 30 30 "----- 0001 0 ,000
31 36 2C 30 30 34 39 39-2C 20 20 20 20 20 2C 30 16,00499,-----,0
30 32 35 31 2C 30 30 30-37 34 2C 30 30 32 32 36 0251,00074,00226
2C 30 30 39 30 30 2C 30-30 32 39 35 2C 30 30 33 ,00900,00295,003
32 37 2C 30 30 31 38 37-2C 30 30 33 39 35 2C 30 27,00187,00395,0
30 30 36 38 2C 30 30 30-32 37 2C 30 30 31 38 38 0068,00027,00188
2C 20 20 20 20 20 2C 30-30 30 31 39 2C 30 30 30 ,-----,00019,000
33 31 2C 30 30 39 35 30-2C 20 20 20 20 20 2C 20 31,00950,-----,-
2C 20 2C 30 2C 30 2C 30-2C 31 2C 31 2C 30 2C 30 ,-,0,0,0,1,1,0,0
9B [checksum]
NOTE: Sample checksum value
may not match this record's data.
[ETX] [end of text]