Kerrigan 2015 Thermal Degradation of Synthetic Cathinones. Journal of Analytical Toxicology 1.GC-MS 4h REARS ERE EIT, HASHES, HERE (2, HAUS FB. Ih, ERE RRRE-RitReeRekaat. ZEEE AUES +S alplha CH betaCZM, AMnte>o AOSRATURS TAT ( ®) , TOR PRARAY Eas ES AED BE F (iminium ions, R1R2-C=N+-R3R4)Rialae F (acylium ions, R-C+=O), LARAB>Bsecondary and tertiary fragmentations. 3. BE SH R3, R4, RSAUCHRE, StRA ABR MIRNA EE Ue (Ze 3) , Eee) aE EBS CnH2n+2N+ (i.e. miz 44, 58, 72, 86, 100 etc.), Hi BS SiS EARS ( 23), SATE Rey ethylone Ml butylone MAHasel. ATS TERED IB AREF HER 72, 149, 121) RAR OA RAR (ie. m/z 44 ion) REE -EPRATM 3,4-methylenedioxypyrovalerone (MDPV), naphyrone, 4-methyl-alpha-pyrolidinobutiophenone (MPBP), 3,4-methylenedioxy—alpha-pyrolidinobutiophenone (MDPBP), and pyrovaleroneBJHat SRAM F MR, AOR VORRS SAR, ALERT EU Rea BRR aSeS, EDT RAMA BAFEMAYSERRTASRSHEVERHLRPH, KRRNREA ESE, MRP RATS SEBMorad(24)SuR, bifid pHAbIAICAthinonelA RAK PRRBRIZ, BAP aulsRsis , Fi REBBALREEALMU EN HWOTER, RRR S PASS AEA MEMSESARASOMST ASE ERE, HF, LAE, sHaRe T MARSA ASE AO REE BATE CL eH i EG CA AE HS ENR BAR RM EAR, HSH Noggle (30) and DeRuiter (31) Air HS, ELAS HORS RAA CERES SPE, MHAL, KALE S SERS A SM EBS aS Re ES pl LAE A(32), cathinone AS ETEE RIE B RFE Matcathine, —tcathinone—k, FAMAETHRBA RH Met cathinest; (+)norpseudoephedrine. BATES RAREE, AUER MFO MT ROSES SAR, cathinonetha Aes RELIES, GLIETE(eAe3, 6—dimethyl-2,5—diphenyldihydropyrazine LAR Atta 3,6-dimethyl-2,5-diphenylpirazine ie SHAE BA ace (33), BI, cathinonez#iae es dimerization, Tae HVEIRe ais th BE dimerization (34). AEM SRS PAIRS WAiso—cathinones , 1 AKASMblemephedrone, iso-cathinonesiRAS MR WAviBE ( 35), 318A, HIE timethcathinone & ethcathinones LAR pentedrone Arz8) iso-cathinones ZHisi7tH, wae wT yey VARS ERA 35 a B(35-36),, - HARADA AL IMSHR, RULER EECCORHEENAER AED, Is TaAe ERNAF, SHBTRROTRRR ESSE, MAA ESI HRRS + ,Materials and methods BREA TAC RPS AE IT EE BRO PNA, (IANS ETH ERSPCP EAS oe RAUL ee, 1, Agilent 5975 MSD/ 7890 GC equipped with DB-5MS column (30 m x 0.25 mm x 0.25 um) 2. initlal / optimized injector and interface temperature were made at (260, 280 C)/ to (175, 280 C) 3. 2ul injection were made in split ratio at 25:1 using a Sil-tek deactivated cyclosplitter liner (4x 6.3 x 78.5 mm ) (Restek) and Ultra Inert Gold Seal (Agilent) 4. Data were aquired at full scan aquisition (40-650 Da) 5. Optimium separation pf the cathinones was achived using an initial oven temperature of 140 C (held for 5 min) followed by a 10 C/min ramp to a final temperature of 265 C. 6. When analyzing biological extracts for cathinoes, a post-run burnout at 290 C (2.5 min) was incoprated for a total cycle time of 15.5 min. Helium used as the carrier gas ar a flow rate of 1.3 mL/min. Prior to optimization, temperature programming was identical with the exception of 30 C/ min temperature ramp.sR (p 6) EAEARBE?, BRIM+CGREHUARRABSAR, THR 1. mixed or unresolved artifacts flephdrone, ethcathinone, buphedrone, pentedrone, 4-MEC, ethylone, butylone and pentylone 2. partially (near baseline) resolved artifacts mephedrone, 4-EMC and methcathinone 3. fully resolved artifacts methylone, methedrone, MPBP, MDPV, MDPBP, pyrovelone and naphyrone #£extracted ion chromatograms (m/z 58, 56)#8ztF, AaBSHIREF HS HBB PERT Be ( -2 Da), RERRADRMT (At) EMR THEARF, Witpartially and fully resovied (AWM ATHUAGR , RBA RMA ieee, IRANI R tay EM DPVER a, 40-2 Da ESA SEAM IRA PRBES |V, RELBLRS , (RO WELRRED FREF (4/1 8B) LE, SBIR, TMM RRR ALS REEL A I PR SEIS ay B18 kahaiartifactsH#2e, GABA BARRENSRT ATH, ASR fa (0.1 Mg/ML) BARRE AEBS ie eS BS RR AE on (PCP ESA RM PIETS REM, (Aa SRT RAAB Mea eRZ artifacts, BARAT AR, l—esslA OAR TT MANTA OEMABKAALE, gold sealkGtuetlt ERPCCHMNSHLBAM, BRULMLER, eee es SSIS CS ER ISD, EOE STB RARE TA AP OR 1 O28 71s A230, 290 C teas, set we URE ESE HAUSE, SeEARIOS AMARA MAE festeHa ED ST MAR ESRI, RSA TRB MARE ESE, STRELA EVEL EEE. AIC RRMS VS. ABIMDPV (10.86 min)eBeHas, FIRE (11.25 min TARAS, MMDPV-enamineSiSHRAA MAR Ree we SHR RARE; AA MES, MDPV-enamine (44 AI10% , MAE RE SHIRE BUKS% FLEAS ALEVE ATLAS (4.7-5.5% ) , MOPIAT ERAS LEAS 1:25.ata HAE AE MOL CCHS EF MRO ARS, (iARHISES, Noggle(30) and DeRuitter(31) 8 258—{izsaFlmethcathinone RH VERA LARS, alee eA BRS RAG SEAS GHEE RRA SR RES, APS Ms Re, RRMALARRERKA CASES, BtSTSORF AR. RiAlbWRS Archer A, 7 fluoromethcathinone siZE SSM LEARTsujikawa SATA alpha-PVPLE MRE, SARTRE RSE FE EBS iets Se B2BRSFBLORERAZ Da. RPGR YOR Fit tee ey mE Rae AR ( conjugated or unconjugated) aE 11PRa, TERR HASAAZASRS, MRI RRAAS EF, Noggle and DeRuitter@s—(zHEw methcathinone2 ais, ARAABHERIBIE, H44N,N-dimethylemthcathinone R diethylcathinone (diethylpropion) #78, ASRS RRR RAR, Alt, ERMAN RMIT ERR, LAL AUER REA. TBR OTRETF ALLIES. Tsujilkawa thifsialpha-PVPRAM EA WAI EY ERED CARLES. Ath HS a RS IL RACSREBE BE MAES RA, TEAIMET, BRESHEIR (200-300 CaP HE FERS, BAST alpha-PVPHBHAS, ALR, EHC REAR Lioe BZA, SWGDRUG 2-fluoromethcathinone this HA Aas (+iAG58 (mS ASCH, AIR FA#IR(39), Archer(38) #58 AGC MS34f14—-methmethcathinone (mephedrone) & fluoromethcathinoneti ie Ri, RMR, HRA AS4—fluoro, 3-fluoro and 2-fluoromethcathinone, FEZ ERARRH EM RNS SS BEAR, BP: RAPER (THE ( ARCO RRS ) MR, TEATLUR MORSE, (SRS RAAIS RRR ANE A ABATE fe, ARRAS. SHERPA A MAELO WGCMSAAURES, SPATTER MASEL, (BIES aber BSTSAiTL, HRUARHTARASR RSH, GARE —ER WAS, Sa 2ie THESE, AACN ARERSHES, RGR ORAN DH, (AGE AR Fe Has mA, TAH SRE. HRP RACES. Sa (1 PAA tiem ) Mab, (SSAA FORE TSMR OTSRLRS SASH