1.

When moving your microscope, always carry it with both hands

(Figure 1, at left). Grasp the arm with one hand and place the
other hand under the base for support.
1. Make sure the lowest power objective lens is "clicked" into place
with the revolving nosepiece (This is also the shortest objective
lens).
2. Place a coverslip or cover glass over the specimen to prepare your microscope
slide. If the objective lenses come into contact with the slide, this will help protect
them. Place the microscope slide on the stage and use the stage clips to secure
it. To open the stage clip, press down on the back end of it.
3. Turn the coarse focus knob so that the objective lens moves
downward as you look at the objective lens and the stage
from the side (Figure 2, at right) (or the stage, if it moves,
goes upward). Without touching the slide, move it as far as it
will go!
4. Now, look through the eyepiece and adjust the illuminator (or
mirror) and diaphragm to get the most light (Figure
3,Microscope Viewingat left).
5. Slowly turn the coarse adjustment so that the objective lens
goes up (away from the slide). Continue until the image comes into focus. Use
the fine adjustment, if available, for fine focusing.
6. Move the microscope slide around so that the image is in the center of the field of
view and readjust the mirror, illuminator or diaphragm for the clearest image.
7. Now, you should be able to change to the next objective lens
with only minimal use of the focusing adjustment. Use the fine
adjustment, if available. If you cannot focus on your specimen,
repeat steps 4 through 7 with the higher power objective lens in
place. Do not allow the objective lens to touch the slide.
8. The proper way to use a monocular microscope is to look
through the eyepiece with one eye and keep the other eye
open (this helps avoid eye strain). If you have to close one eye
when looking into the microscope, it's ok. Remember, everything is upside down
and backwards. When you move the slide to the right, the image goes to the left!
9. Do not touch the glass part of the lenses with your fingers. Use only special lens
paper to clean the lenses.
10. When finished, raise the tube (or lower the stage), click the low power lens into
position and remove the slide.
11. Always keep your microscope covered when not in use.
Microscope slide It is a thin, flat piece of glass that is made from the highest-quality
glass and typically 75 x 25 mm and 1 mm thick or 3 x 1-inch x 1 mm. Some slides are
thicker, but most people use 1 mm thick. Therefore, in order to prepare it we must do
with carefulness and must pay attention and although there are a lot of ways to prepare
it for each situation, we will go with each of them:
 Wet Mount Slides: Living samples, translucent liquids, and aquatic samples are all
mounted using wet mounts. The moist mount is similar to a sandwich. The slide is
the bottom layer. The liquid sample comes next. To reduce evaporation and protect
the microscope lens from the sample, a tiny square of clear glass or plastic (a
coverslip) is placed on top of the liquid.
1. lace a drop of fluid in the middle of the slide (e.g., water, glycerin, immersion
oil, or a liquid sample).
2. If viewing a sample not already in the liquid, use tweezers to position the
specimen within the drop.
3. Place one side of a coverslip at an angle so that its edge touches the slide
and the outer edge of the drop.
4. Slowly lower the coverslip, avoiding air bubbles. Most problems with air
bubbles come from not applying the coverslip at an angle, not touching the
liquid drop, or from using a viscous (thick) liquid. If the liquid drop is too large,
the coverslip will float on the slide, making it hard to focus on the subject
using a microscope.

Some living organisms move too quickly to be observed in a wet mount. One solution is
to add a drop of a commercial preparation called "Proto Slow." A drop of the solution is
added to the liquid drop before applying the coverslip.

 Dry Mount Slides: Dry mount slides can consist of a sample placed on a slide or else
a sample covered with a coverslip. For a low power microscope, such as a
dissection scope, the size of the object isn't critical, since its surface will be
examined. For a compound microscope, the sample needs to be very thin and as flat
as possible. Aim for one cell thickness to a few cells. It may be necessary to use a
knife or razor blade to shave a section of the sample.

1. Place the slide on a flat surface.

2. Use tweezers or a forceps to place the sample on the slide.
3. Place the coverslip on top of the sample. In some cases, it's okay to view the
sample without a coverslip, as long as care is taken not to bump the sample into
the microscope lens. If the sample is soft, a "squash slide" may be made
by gently pressing down on the coverslip.
 Staining Cellular Specimens slides: There are many methods of staining slides.
Stains make it easier to see details that might otherwise be invisible.ASimple stains
include iodine, crystal violet, or methylene blue. These solutions may be used to
increase contrast in wet or dry mounts. To use one of these stains:

1. Place a paper towel sheet against one edge of the cover slip. Set the towel
against the slip’s edge without disturbing the material under the cover slip. The
absorptive paper towel will draw some of the water out from under the cover slip,
and pull the staining agent under the cover slip and onto the specimen.
2. Place 1 drop of iodine or methylene blue on the other side of the cover slip. Use
an eyedropper and drop the staining chemical on top of the microscope slide,
directly next to the cover slip. Be careful to only dispense 1 drop. Excess staining
agent may run off of the slide
3. Wait while the staining agent is drawn under the slide cover. The staining agent
will begin to seep under the cover slip as the paper towel draws water out from
the other side. It may take as long as 5 minutes for the iodine or methylene blue
to soak completely under the slide cover and saturate the specimen.
4. Wipe up excess staining agent with a clean paper towel. 

https://www.thoughtco.com/how-to-prepare-microscope-slides-4151127

https://microscope-microscope.org/microscope-info/how-to-use-a-microscope/