Abbott: CELL-DYN 1800 Automated Hematology Analyzer Service & Support Manual

ABBOTT
CELL-DYN 1800
Automated Hematology Analyzer
Service & Support Manual
ABBOTT CD1800 SM
Theory of Operation ............................................................................................................................................................................ 4
System Overview.................................................................................................................................................................... 4
Major Subsystem Descriptions........................................................................................................................................... 6
Circuit Descriptions ............................................................................................................................................................. 15
Signal Processor Module (SPM)....................................................................................................................................... 18
Cell Count Module (CCM) ................................................................................................................................................... 21
Troubleshooting................................................................................................................................................................................. 31
Troubleshooting Charts...................................................................................................................................................... 33
Raw Data Description.......................................................................................................................................................... 40
CCM On-Board Diagnostic LEDs...................................................................................................................................... 41
CPU Hardware/Software Configuration .......................................................................................................................... 44
Service Special Commands............................................................................................................................................... 45
Sample Probe Description ................................................................................................................................................. 49
CELL-DYN 1800 Error Messages...................................................................................................................................... 60
Software Commands and Sequence ............................................................................................................................... 72
Engineering Drawings and Schematics ............................................................................................................................................. 76
CELL-DYN 1800 PCB Reference .................................................................................................................................................... 77
Removal & Replacement .................................................................................................................................................................. 96
Service Equipment Required............................................................................................................................................. 96
Covers (CD18-A1) .................................................................................................................................................................... 97
Flow Panel (CD18-B1)............................................................................................................................................................ 105
Fluid Power Supply (CD18-C1).............................................................................................................................................. 115
Syringe Assembly (CD18-E1)................................................................................................................................................. 119
RR-E1.04 Sample Syringe Driver Assembly .................................................................................................................... 125
RR-E1.06 Lyse Syringe Driver Assembly..................................................................................................................... 127
Electronics / Card Cage (CD18-F1) ........................................................................................................................................ 129
RR-F1.01 PAM (Pre-Amplifier Module) ............................................................................................................................. 129
RR-F1.02 MPM (Motor Processor Module) Board .......................................................................................................... 131
RR-F1.03 CDM (Cable Distribution Module) Board ........................................................................................................ 133
RR-F1.04 Hard Disk Drive................................................................................................................................................... 135
RR-F1.05 Floppy Disk Drive ............................................................................................................................................... 138
RR-F1.06 Signal Processor Module (SPM)...................................................................................................................... 140
RR-F1.07 Cell Count Module (CCM)................................................................................................................................. 142
RR-F1.08 Data Link Adapter (DLA) ................................................................................................................................... 143
RR-F1.09 Single Board Computer (SBC) ......................................................................................................................... 145
RR-F1.10 Card Cage Backplane PCB .............................................................................................................................. 147
LCD/Keyboard (CD18-G1)..................................................................................................................................................... 149
Power Supply (CD18-H1).................................................................................................................................................... 151
Verification Procedures ................................................................................................................................................................... 154
VP-01 Preparation for Alignment and Verification ................................................................................................................. 154
VP-01 Preparation for Alignment and Verification..................................................................................................... 154
Test Equipment and Supplies Required....................................................................................................................... 154
Preparation for Alignment/Verification ......................................................................................................................... 155
Order of Alignment/Verification...................................................................................................................................... 156
VP-02 Decontamination .................................................................................................................................................... 157
VP-03 Vacuum and Pressure Adjustments.................................................................................................................. 158
Regulator Alignment.......................................................................................................................................................... 159
Pressure Adjustment (0.5 psi)......................................................................................................................................... 160
Pressure Verification (High) ............................................................................................................................................ 161
Vacuum Adjustment (8 inch) ........................................................................................................................................... 161
VP-04 Metering System Timing Adjustments - RBC and WBC .............................................................................. 162
Metering Timing Fault Report.......................................................................................................................................... 163
RBC Metering System Timing Adjustment .................................................................................................................. 165
WBC Metering System Timing Adjustment ................................................................................................................. 166
VP-05 CMOS Setup Verification/Adjustment............................................................................................................... 167
VP-06 Card Cage Backplane Test Points ..................................................................................................................... 171
VP-07 Cable Distribution Module Test Points............................................................................................................. 172
VP-08 Pre-Amplifier Module (PAM) Adjustment ......................................................................................................... 173
VP-09 Signal Processor Module (SPM) Verification/Adjustment ........................................................................... 176
RBC Gain .............................................................................................................................................................................. 178
RER Adjustment ................................................................................................................................................................. 179
WBC Gain ............................................................................................................................................................................. 182
PLT Gain ............................................................................................................................................................................... 184
VP-10 Diluent and Sample Verification/Adjustment ........................................................................................................ 187
Diluent Volume Verification ............................................................................................................................................. 188
Sample Volume Verification ............................................................................................................................................ 188
VP-11 Stepper Motor Power Test and Verification .................................................................................................... 189
VP-12 Sample Probe Alignment Check ........................................................................................................................ 190
VP-13 Serial Transmit to LIS Verification..................................................................................................................... 191
VP-14 Hard Disk Drive Setup and Verification............................................................................................................ 194
VP-15 Software Installation/Upgrades .......................................................................................................................... 197
Theory of Operation
System Overview
The CELL-DYN 1800 Automated Hematology Analyzer is a complex system. Analyzer performance depends on several
components that together make up the complete hematology system. The system is comprised of the following components
and subsystems:
• Flow Panel System [3]

• Fluid Power Supply [2]
• Reagent Inlet Panel [6]
• Syringe Drive Assemblies [7]
• Electronics Card Cage [8]
• LCD Display System [4]
• Touchpad (Membrane) Keyboard [5]
• Power Supply Assembly [1]
Flow Panel
The Flow Panel consists of tubing, solenoid valves, and other hardware components used for sample aspiration, dilution,
measurement and waste removal.
Fluid Power Supply
The Fluid Power Supply contains the vacuum and pressure pumps, accumulators, waste bottles, and associated solenoids
and hardware.
Reagent Inlet Panel
The Reagent Inlet Panel provides connections for incoming reagents and outgoing waste. The Lyse solenoid is also mounted
on this panel.
Syringe Drive Assembies
The Syringe Drive Assemblies include the Sample Syringe for aspirating samples, the Diluent Syringe for supplying Diluent
throughout the Flow Panel, and the Lyse Syringe for dispensing Lyse to the WBC transducer for the HGB measurement
process.
Electronics Card Cage
The electronics card cage, with associated PCBs, provides command and control signals for the various electronic
components of the instrument. This assembly contains the Backplane PCB, Cell Count Module, Signal Processor Module,
Data Link Adaptor and Single Board Computer.
LCD Display Screen and Keyboard
The LCD Display Screen provides a visual data display and the keyboard provides data input by the operator.
Power Supply Assembly

The Power Supply Assembly provides an AC and DC voltage source to various components on the CELL-DYN 1800 System.
The DC Regulator PCB provides power to the Backplane PCB for use with the digital circuitry on various PCBs. It also
provides power to the Cable Distribution Module and fans.
The AC Regulator PCB provides power to the Backplane PCB (for use with the analog circuitry on various PCBs). It also
provides power to the Pump Relay Module.
The +28VDC Switching Power Supply provides power for the stepper motors via the Motor Processor Module. It also
provides power to initially energize solenoids.
Major Subsystem Descriptions
To aid in understanding the overall system, the electronic modules are divided into the following major functional
subsystems:
• Data Interface and Control Subsystem

• Measurement Subsystem
• Solenoid Motor and Pump Subsystem
• Single Board Computer Subsystem
• AC and DC Power Distribution Subsystem
Data Interface and Control Subsystem
The purpose of this subsystem is to interface the user data, control data, measurement data, and system status data in the
system. This data is connected via four independent data busses:
• DLA/CCM (Data Link Adapter/Cell Count Module)

• CCM/SPM (Cell Count Module/Sample Processor Module)
• CCM/CDM (Cell Count Module/Cable Distribution Module)
• CCM/MPM (Cell Count Module/Motor Processor Module)
See the figure below for a diagram showing the data connections.
Data Interface and Control Block Diagram

When power to the instrument is turned ON, the system is operating software is loaded from the hard disk into RAM on the
SBC (Single Board Computer). The SBC then uses various handshaking signals and data bytes to communicate with the
CCM (Cell Count Module) via the DLA (Data Link Adapter).
The CCM functions as the master controller with all system functional commands residing in firmware (PROM). The CCM
sends control data and receives status data from the CDM (Cable Distribution Module).
The CCM provides current control to the von Behrens RBC and WBC Transducers and the two metering PCBs and serves as
the system's analog voltmeter for use in converting the HGB signal. Data is written and read via the CCM/CDM and
CCM/MPM data buses.
The CDM (Cable Distribution Module) acts as a controller for the solenoids and also interfaces data from various system
sensors.
The MPM (Motor Processor Module) acts as a controller for all Stepper Motor Drive PCBs.
Measurement Subsystem
The measurement subsystem provides detection, amplification, and processing of the signals from the von Behrens
RBC/PLT Transducer, von Behrens WBC Transducer, and HGB Flow Cell. RBC/PLT and WBC metering is also included in
this subsystem.
See the figure below for a diagram of the measurement process.
Measurement Block Diagram
The PAM (Pre-Amplifier Module) supplies constant current for the von Behrens RBC/PLT and WBC Transducers and HGB
LED voltage.
The RBC/PLT and WBC cell pulses are input to the PAM where they are amplified and routed to the SPM (Signal Processor
Module).
When the SPM receives signals from the RBC/PLT and WBC, the following occurs:
• The RBC/PLT signal is amplified (gain) and split into independent RBC and PLT signals.
• The WBC signal is amplified (gain).
• The RBC signal is routed to the cell editing circuitry.
• Cell editing is performed on the RBC signal to eliminate invalid RBC pulses.
The SPM discriminates cell size by converting pulse height to a proportional digital value. The amplitude of each valid pulse
is measured by a fast A/D converter then sent across the data bus to the CCM.
The A/D data for RBC, PLT, and WBC are individually divided by the CCM into 256 discrete size channels. The cell count in
each channel is accumulated in discrete memory locations and is used to generate count data, percentage data, and
histogram data for RBC, PLT, WBC, and other derived parameters.
Signals from the upper and lower detectors on the RBC/PLT and WBC metering PCBs are converted to TTL levels by
comparators on the CDM. The signals are then routed to the CCM where they are used to control RBC/PLT and WBC
sample timing.
The HGB analog signal from the HGB Flow Cell is captured by the PAM where it is amplified and routed to the CCM. The
HGB signal is then measured and converted to a digital format by a voltmeter-A/D converter.
Solenoid, Motor Drive, and Pump Subsystem
Solenoid control commands reside in firmware on the CCM. These commands are sent to the CDM where they are
multiplexed to the appropriate SDM (Solenoid Drive Module). The SDM then provides the current to open and close
individual drive solenoids.
Stepper Motor commands are handled in much the same manner as described above. However, the final multiplexing of the
Stepper Drive PCBs is controlled by the MPM.
There are two pressure pumps and one vacuum pump in the CELL-DYN 1800 System. These pumps are described as
follows:
• A pressure pump provides air to bubble-mix samples in the Pre-Mixing Cup and the mixing chambers of the von
Behrens RBC/PLT and WBC Transducers. A pressure regulator regulates the 0.5 psi in the pressure accumulator for
this process.
• An unregulated pressure pump provides air to push waste from the waste bottles inside the instrument to the waste
container attached to the instrument and to apply back pressure to clear the apertures in the von Behrens RBC/PLT
and WBC Transducers.
• An 8" Hg vacuum accumulator, vacuum sensor, and vacuum pump supply a constant vacuum to the entire system to
transport Diluent, Detergent, and Lyse throughout the flow system and to maintain a constant vacuum to the
RBC/PLT and WBC metering tubes. A vacuum regulator maintains a constant vacuum source to both metering
tubes.
See Solenoid, Motor Drive and Pump Block Diagram for a diagram of the solenoid and motor drive connections.
Solenoid, Motor Drive and Pump Block Diagram

Single Board Computer Subsystem
The Single Board Computer subsystem consists of the following components:
• Single Board Computer PCB

• Data Link Adapter
• Input/Output Ports (serial/parallel)
• Keyboards (PC and membrane)
• LCD Display Screen
• Disk Drives (hard and floppy)
The figure below illustrates the major components of the User Interface Computer.
User Interface Computer
SBC (Single Board Computer) PCB
The Single Board Computer (SBC) PCB is a complete Celeron 850 Megahertz PC computer system with 128 Megabytes of
RAM that is self-contained on one board. It utilizes a PC compatible BIOS with DOS capability that directly interfaces with the
LCD Display Screen, Hard Disk Drive, Floppy Disk Drive, PS/2 Keyboard, one (1) Parallel Port and two (2) Serial Ports. It
connects directly into the Backplane PCB along with the Data Link Adapter (DLA) and utilizes the ISA bus and Backplane
PCB to communicate with one another.
DLA (Data Link Adapter)

The DLA PCB connects directly into the Backplane PCB. It provides a program-controlled data channel from the SBC to the
CCM. The DLA performs the following three functions:
• Receives measurement and analyzer status data from the CCM

• Transfers commands from the SBC to the CCM
• Sends control data from the UIC to the CCM.
Serial I/O Ports
The CELL-DYN 1800 contains two (2) Serial I/O Ports for transferring data to other computer systems:
COM1 RS-232 Data Output
COM2 Spare
Parallel I/O Port
The CELL-DYN 1800 contains one parallel I/O port for transferring data to a printer.
PC/2 Keyboard
The external 101 key PC keyboard is used to enter alphanumeric data, such as demographic information, into the instrument.
Touch Pad (Membrane) Keyboard
The touch pad (membrane) keyboard is located below the LCD Display Screen. The keyboard includes a row of eight (8)
unmarked rectangular-shaped keys corresponding to labels displayed at the bottom of the screen. These keys activate the
indicated function or display the indicated submenu.
LCD Display Screen (Color)
The LCD Display Screen has the following characteristics:
• Size: 8.5 x 6.4 inches (10.4 inches measured diagonally)

• Number of colors: 16
• Pixels: 640 width x 480 height (or 800 x 600)
• Backlight on/off control (software controlled screen saver)
An LCD Adapter, connected directly on the SBC PCB, drives the LCD Display Screen. The adapter supports SVGA 640 x
480 and 800 x 600 graphics modes.
Hard Disk Drive
The hard disk drive stores the User Interface Software program and the Patient Data Log.
Floppy Disk Drive
The 1.44MB 3.5" floppy disk drive is used for program installation and provides the QC (Quality Control) data
upload/download capability.
Printer
The User Interface Software supports Epson ESC-P or PCL-3 languages. The printer has its own buffer and is capable of
printing on 8.5" x 11" (letter size) or A4 paper size. The printer supports alphanumeric and graphics reports from stored data
and screen displays.
Speaker
The PC speaker or buzzer is controlled by software and is mounted on the SBC PCB.
AC and DC Power Distribution Subsystem

The Power Supply Assembly is comprised of three components: AC Regulator PCB, DC Regulator PCB and +28VDC
Switching Power Supply. These components are mounted together as an assembly and are located at the right/rear side of
the instrument.
When the system is turned on, the AC line is routed into the AC Regulator PCB and +28VDC Switching Power Supply. The
AC Regulator PCB automatically accommodates line voltages of 90 - 130VAC and 200 - 260VAC by sensing the input
voltage and utilizing an internal comparator bank and power transformer to produce the 120VAC necessary for the
subsystem's function. The Power Supply Assembly then provides an AC and DC voltage source to various components on
the CELL-DYN 1800 System.
+28VDC Switching Power Supply
The +28VDC Switching Power Supply provides the voltage source to the DC Regulator PCB, which the board then uses to
produce other voltages. The power supply cooling fan is thermistor controlled, which means that when the internal
temperature rises above 70°C, the fan operates at full speed. The fan is then turned off at 50°C and kept off until the
temperature rises above 70°C.
AC Regulator PCB
The AC Regulator PCB provides the 120VAC used by the Pump Relay Module (PRM) for vacuum and pressure pump
operation.
The ±12VDC (analog) is provided to the Backplane PCB, which is used by the Cell Count Module (CCM) and Signal
Processor Module (SPM). The ±12VDC is also provided to the Cable Distribution Module (CDM), Motor Processor Module
(MPM) and Pre-Amplifier Module (PAM) (for its circuitry).
The +100VDC is provided to the PAM for use in its constant current circuitry. In between the AC Regulator PCB and the PAM
is the Pre Amp Filter that is used to filter out noise.
DC Regulator PCB
The DC Regulator PCB provides +5VDC, ±12VDC (digital), +14VDC and +28VDC. The +5VDC is provided to the Backplane
PCB, which is used by the SPM, CCM, Data Link Adapter (DLA) and Single Board Computer (SBC). This voltage is also used
by the CDM, MPM, Hard and Floppy Disk Drives.
The ±12VDC (digital) is provided to the Backplane PCB for use on the CPU fan. The Hard and Floppy Disk Drives receive
+12VDC for their operation.
The +14VDC is provided through the CDM to the Solenoid Driver Modules (SDM) and is used to hold the solenoids closed or
open for normally closed solenoids.
The +28VDC is provided to the MPM for operating stepper motors and through the CDM to the SDMs to initially close
solenoids or open for normally closed solenoids. This voltage is also used for the system's internal cooling fans. The cooling
fans are thermistor controlled, which means that when the ambient temperature inside the instrument drops below 25°C, the
fans operate at half speed. Once the temperature rises above 25°C, the speed is increased linearly until it reaches 35°C,
when the fans operate at full speed.
The DC Regulator PCB also provides +5VDC and +14VDC to the AC Regulator PCB.
Circuit Descriptions
This section contains a description of the circuitry for the following printed circuit boards:
• Pre-Amplifier Module (PAM)
• Signal Processor Module (SPM)
• Cell Count Module (CCM)
• Cable Distribution Module (CDM)
• Solenoid Driver Module (SDM)
• Motor Processor Module (MPM)
• Stepper Drive Printed Circuit Board (SDP)
• Pressure/Vacuum Regulator Module (PVRM)
• Power Supply Assembly
• Pump Relay Module (PRM)
• Single Board Computer (SBC)
• Data Link Adapter (DLA)
Pre-amplifier Module (PAM)
Note
Refer to PAM PCB Diagram.
PAM PCB Diagram

The PAM performs the following functions:
• Provides constant current control to the von Behrens RBC/PLT and WBC Transducers.
• Amplifies the initial RBC/PLT, WBC and HGB signals.
The constant current bias (+100VDC) is switched by U6, then routed to Q2 and Q3, which supplies constant current to the
von Behrens RBC/PLT transducers. The RBC/PLT current is adjusted with R72. Once received, the RBC/PLT signals are
initially amplified by U7, then routed to U5, where they are re-inverted and further amplified. The combined RBC/PLT signal is
then routed to the SPM PCB.
Transistors Q4, Q5 and associated circuitry provide constant current for the von Behrens WBC Transducer. The WBC
current is adjusted via R66. Once received, the WBC signal is initially amplified by U12, then routed to U11 where it is
re-inverted and further amplified. The WBC signal is then routed to the SPM PCB.
The output of the HGB Flow Cell is amplified by U1 and U2 prior to being routed to the SPM PCB. The HGB self test and gain
voltages are adjusted with R14 and R4 respectively.
Signal Processor Module (SPM)
Note
Refer to SPM Architecture.
SPM Architecture
EPLD (SPM)
The EPLD is an enhanced programmable logic device. The SPM EPLD is used to control data acquisition.
There are three state machines that run in the EPLD. One each is used for controlling data acquisition elements such as
Multiplexers and Peak/Hold Amplifiers. The inputs to the EPLD state machines are the outputs of the various Threshold and
Slope Detectors as well as Bubble and Area Comparators.
There is a data transfer state machine which controls data flow to the CCM.
Signal Processing
There are two signals from the RBC and WBC transducers that are processed through two basic data acquisition circuits.
The Platelet signal is derived from the RBC signal. There are three gain adjustments associated with each of these signals.
In addition to the three gain adjustments there is a integration adjustment for detecting pulses that are too long.
WBC Signal
• The WBC signal is received through a differential amplifier to reduce noise. There is a gain adjustment after the
differential amplifier but before the Test Signal injection point.
• The WBC signal is then "Baseline Restored" to remove DC components and Baseline fluctuations due to varying
duty cycle of the blood cells.
• A threshold detector signals to the EPLD the presence of valid pulses.
• Simultaneously the Bubble detector signals if the pulse is determined to be a bubble. The bubbles are much larger
than pulses from blood cells. The EPLD discards the information from the pulse if it is determined to be a bubble.
• The data from the pulse is processed on the falling edge of the Threshold Detector unless the Slope Detector senses
another rising edge before the falling edge of the Threshold Detector. The data is processed immediately if there is
another rising edge before the falling edge of the Threshold Detector.
• The WBC Held Peak is converted and sent to the CCM on a 15µS cycle. Average pulses from the impedance
transducers are 35µS.
RBC and PLT Signals
There are two modes for RBC/PLT data acquisition:
• RBC MCV
• RBC Count/PLT
RBC MCV
RBC MCV is for RBC MCV data only. In this mode, an integrator is enabled to determine if the cell is moving through the
middle of the aperture. If the cell is not moving through the center of the aperture, the data is falsely high and the pulse is
abnormally long. The integrated signal is compared to a proportion of its height. If the pulse is too long for its height, it is
discarded.
RBC Count/PLT
RBC Count mode is for count information. During this run, Platelets are counted simultaneous with RBCs and no integration
is used. The Platelet signal is derived from the RBC signal by an adjustable gain.
• The RBC signal is received through a differential amplifier to reduce noise. There is a gain adjustment after the
differential amplifier but before the Test Signal injection point.
• The RBC signal is then "Baseline Restored" to remove DC components and Baseline fluctuations due to varying duty
cycle of the blood cells. At this point an additional gain stage is added to create the Platelet signal.
• A threshold detector(s) signals to the EPLD the presence of valid pulses. The Platelet Threshold Detector is used if in
RBC Count mode.
• If in Count mode, the Platelet Lower and Upper Threshold detectors are used. If the Upper Threshold Detector is
triggered, the pulse is a RBC and the RBC pulse is accumulated into the RBC Count histogram. If only the Lower
Detector is triggered, the PLT pulse is accumulated into the PLT histogram.
• The data from the pulse is processed on the falling edge of the Threshold Detector unless the Slope Detector(s)
senses another rising edge before the falling edge of the Threshold Detector. If this occurs, the data is processed
immediately.
• The RBC Held Peak or the PLT Held Peak is converted and sent to the CCM on a 15µs cycle.
Cell Count Module (CCM)
Note
Refer to PAM PCB Diagram.
CCM Architecture
System Clock and Microprocessor Description
The CCM uses a 16 MHz crystal as a time base. The fundamental cycle time for the MC68HC11KW1 processor is 4 MHz.
The processor has many built in functions such as:
• 16 bit address bus

• 8 bit data bus
• 7 - 8 bit multi-purpose I/O ports (CCM and SBC ports)
• Small amounts of Internal RAM and Internal EEPROM (analyzer serial number)
• Internal Timers (system timer)
• Internal UART (debug port)
EPLD (CCM)
The EPLD is an enhanced programmable logic device and is used for address decode and histogram building functions. Its
program is used in coordination with the processor software.
LEDs
Note
Refer to CCM On-Board Diagnostic LEDs.
Scratch Pad RAM
This RAM is used for general purpose programming.
Histogram Memory
This RAM is used for histogram building.
Histogram Building (cell counting)
The following sequence is executed to build histograms:
1. Histogram Memory is cleared.

2. Counting is enabled.
This disconnects Histogram Memory from the processor buss.
3. Wait for an End of Conversion (EOC) signal from SPM.

4. At the EOC, data is read from the SPM ADC
5. The data then becomes the address for the histogram memory.
6. The data from the histogram memory is read.
This data is the count information for the respective pulse height.
7. The count is incremented and stored back into histogram memory.

8. Counting is disabled. And the histogram can be read by the processor.
HGB Measurement
A 12 bit ADC with an input multiplexer is used to measure the HGB signal from the PAM. This data is read by the processor.
Along with the HGB measurement, various DC voltages are read by the 12 bit ADC as a diagnostic.
CDM Port
The CDM port is connected directly to microprocessor ports E, G and K.
SBC Port
The SBC port is connected directly to microprocessor ports J and part of port A.
Cable Distribution Module
Note
Refer to Solenoid, Motor Drive and Pump Block Diagram.
Solenoid, Motor Drive and Pump Block Diagram

The CDM performs the following functions:
• Status Sensor Interface
• Control of Solenoid Driver Module
• Pump Relay Module interface and control
• Start Board (Touch Plate) Interface
The CDM communicates with the CCM via the CCM/CDM data bus at J2. Analog outputs of the Metering Modules are
converted to TTL levels by comparators (U12) and placed directly on the CCM/CDM data bus. Signals from the Pump Relay
board, Probe Position Switches, and Start Board (Touch Plate) are interfaced by Data Drivers (U5, U10).
Data is interfaced to the Solenoid Driver Modules via J32. This data is then multiplexed by One-of-Eight Decoders (U1, U2)
via J3, J4, J6, J7, and J9.
Vacuum and pressure control data is latched by U14 and routed to the Pump Relay Module via J11. Pump status signals
(Vac On, Pres On) are converted to TTL levels by U3 and placed on the data bus by U5.
LED drive signals are routed to the Start Board (Touch Plate) via J17. The start signal enters at J17 and is placed on the data
bus by U5.
Solenoid Driver Module (SDM)
Note
The purpose of the SDM is to provide drive current to the solenoids. Each SDM has eight Darlington drivers (Q1-Q8) which
are individually controlled by data bits (D0-D7) and data latch (U3).
There are two power modes available for each solenoid - activate (+28V) and hold (+14V). This is controlled by the Hi CLK
signal in conjunction with data bits (D0-D7) and the current control latch (U1).
Motor Processor Module (MPM)
Note
The MPM controls drive data to the Stepper Drive printed circuit boards and also provides self-test capability for motor
winding current. The MPM is comprised of the following major circuits:
• Microprocessor
• Program Control EPROM
• I/O Peripheral Interface Adapter (PIA)
• Direct Memory Access (DMA) Control
• Motor Phase Latches
• Motor Current Latches
• Motor Winding Self Test
Control functions of the MPM are performed by microprocessor (U5).

The operating program for the microprocessor is stored in Program Control EPROM (U3).
Data communications between the CCM and MPM are controlled by I/O PIA (U6) and serial data is interfaced via ACIA (U2)
and Data Bus Connector (J1).
Phase data, motor direction, and step rate are stored in RAM (U7). This data is sent to the Motor Phase Latches under
control of the DMA Control circuitry, which consists of U11, U12, U15, U16, U18, U21 and associated circuitry. The data is
strobed into the appropriate Motor Phase Latch by ALG0 through ALG2.
The Motor Phase Latches U23, U26, and U29 provide phase data to the Stepper Drive printed circuit boards. Each is an 8-Bit
Addressable Latch which can control up to four Stepper Drive printed circuit boards and subsequently four Stepper Motors.
Four levels of motor current for each motor is controlled by the Motor Current Latches (U22, U25, and U28). Each latch can
control up to four stepper drive printed circuit boards. Data is strobed into the appropriate latch by WR0 through WR2.
The Feedback- and Feedback+ inputs at J3 through J14 are connected, via resistors on the Stepper Drive printed circuit
board, to the stepper motor windings. This allows the circuitry consisting of U30, U31, and U32 to monitor the winding current
during an internal self-test. These values can be read by the CCM to isolate a defective Stepper Drive or Stepper Motor.
Stepper Drive Printed Circuit Board
Note
Refer to MPM section, blocks 20, 23, and 24 of Solenoid, Motor Drive and Pump Block Diagram.
The Stepper Drive printed circuit board consists of two PBL 3717 motor drive chips. Each chip drives a winding of the
Stepper Motor. Bits I0 and I1 are used to control four motor current levels:
• P0 - High Current
• P1 - Medium Current
• P2 - Low Current
• P3 - Current Off
Bits PH0 and PH1 control motor phase and, therefore, direction and step-rate (velocity). Feedback+ and Feedback- are used
to generate a motor self-test.
Pressure/Vacuum Regulator Module
Note
Pressure (or vacuum) is sensed by a transducer that is internally configured as a Wheatstone Bridge. Transistor Q1and
resistors R4 and R5 are used to generate a stable reference voltage for the Wheatstone Bridge. The output of the
Wheatstone Bridge is partially amplified (U1-7), stabilized against long term drift (voltage follower U1-1) and made
offset-adjustable by R18 and associated resistors.
Maximum transducer sensitivity can only be achieved when the output is zero volts at TP-1 and when there is no pressure
differential across the transducer. To accomplish this, R18 is adjusted for zero volts when both transducer inlet ports (P1 and
P2) are open to atmospheric pressure.
In order to maintain the operating point of comparator U2-14 at the fixed 2-volt trip level, it is necessary to maintain the output
of U2-8 within a relatively narrow range. This is accomplished by making the differential amplifier (whose inputs are U1-10
and U2-10) adjustable by selecting 1 of 4 possible jumper positions. A stable reference point for the DC operating level of
U1-8 and U2-8 is established by U1-14 in conjunction with R6 and resistor network RP1.
Measurement of pressure in the range of approximately 0.5 lbs/sq. inch is accomplished by using transducer inlet port P1 in
conjunction with jumper setting A/B.
Vacuum pressure in the range of 8 inch Hg is accomplished by using inlet port P2 in conjunction with jumper setting C/D. The
regulation point for either vacuum or pressure is established by the setting of potentiometer R16.
When the output of comparator U2-14 goes positive, the collector of Darlington transistor Q2 is pulled to ground, thereby
turning on either the pressure or vacuum pump. When the pumps are running, LED DS1 is lit and stays lit until either the
pressure or vacuum increases past the hysteresis point established by R8 of U2-14.
The output of the pressure/vacuum regulator can be inhibited by a logic low at J1-1. This completes the circuit description.
Power Supply Assembly
Note
Refer to major subsystem descriptions of AC and DC Power Distribution Subsystem.
Pump Relay Module (PRM)
The PRM provides drive to the vacuum and pressure pumps, via three Solid State Relays: K1, K2, and K3.
Single Board Computer (SBC)
The Single Board Computer (SBC) is designed around a Celeron 850MHz microprocessor and connects directly into the
Backplane PCB. The SBC receives power from the Power Supply Assembly via the Backplane PCB and receives status and
measurement data from the CCM through the DLA. The board contains EPROM, CMOS, RAM, input/output circuitry for the
interface ports, disk drives (hard and floppy) circuitry, LCD Display Screen Interface, and PS/2 Keyboard connectivity.
Data Link Adapter (DLA)
The Data Link Adapter provides interfaces from the SBC to both the CCM (Cell Count Module) and the Touch Pad
(membrane) keyboard. These two interfaces function independently under user interface software control. However, some of
the circuits on the DLA board are shared. The DLA uses an 82C55 PPI (Parallel Peripheral Interface) IC. Refer to the figure
below for an illustration of the DLA board.
DLA (Data Link Adapter) Block Diagram
Interface to CCM
A 20-pin ribbon cable connects the DLA to the CCM. This interface is a bi-directional, parallel interface that is
software-controlled at both ends. Data is transferred in 8-bit bytes on 8 data lines (D0 - D7) in one direction at a time. The
SBC (Single Board Computer) initiates an inquiry message handshake on a periodic basis or when it has a command to send.
The CCM responds by sending data or replying to the command sent.
The interface is controlled cooperatively by the UIC and CCM according to the state of the handshake signals. Refer to Cell
Count Module (CCM). The UIC always sends data first. The CCM responds by sending data back (if any is available). Thus,
the 82C55 switches its A-port from input to output and back to input during every message transaction.
The key handshake signals are REQ1 and REQ2. REQ2 going low initiates the communication protocol. The CCM responds
by bringing REQ1 low. After the DLA has sent its data, it brings REQ2 high. Then the CCM sends its data.
Each byte received by the DLA generates an IRQ. The application software responds to the IRQ by putting the byte into a
buffer. When the CCM brings REQ1 high, the communication is complete; the DLA returns to an idle state, and port A is set
to input. Both REQ1 and REQ2 remains high until the next message/data transfer.
The two LEDs on the printed circuit board, DS1 and DS2, indicate the active state of REQ2 and REQ1, respectively. They
should always be flickering when the application software is running because the User Interface Software program is
constantly polling the CCM to check its state. (There are some exceptions to this during power up and certain diagnostics/test
modes.)
The I-O address of the DLA board is assigned by four jumpers. The default I-O address (pins 3 - 6 on S1 hard-wired) is: [off
off on off], 340 hexadecimal. The DLA interrupt level is assigned by a jumper.
Jumpers W1 - W6 assign the DLA interrupt to one of the following:
W# IRQ #
W1 IRQ 5 *
W2 IRQ 10
W3 IRQ 10
W4 IRQ 11
W5 IRQ 12
W6 IRQ 15
* Since IRQ 5 is used by the DLA, W1 is hard-wired (this is the default).
Interface to Touch Pad (membrane) Keyboard
A 14-pin ribbon cable connects the DLA to the key panel. The key panel is also polled by the User Interface Software
program. This interface is not interrupt-controlled.
The keyboard is interfaced as a parallel switch matrix circuit with 4 lines out and 8 lines in. Four scan rows are driven active
one at a time via an LS175 latch. Then the columns of the key panel matrix are read from port B of the 82C55. The software
interprets a low active signal as a key is pressed.
The DLA board must be plugged into a 16-bit slot. There are no adjustments on the DLA board.
Troubleshooting
Diagnostics Menu Usage
Utilization of the DIAGNOSTICS Menu enables the operator and/or service representative to identify and correct both
operator-correctable and service-correctable faults. When the computer senses a fault, the message <NOT READY: SEE
DIAGNOSTICS> displays in the System Status Box. The following keys are available in the DIAGNOSTICS Menu.
Level One
• INITIALIZATION: Used to perform an Initialization cycle: returns movable components to home position and
performs internal self-tests.
• RAW DATA: Used to display raw measurement data for the last specimen.
• COUNT TEST: Used to run specimens without returning to RUN Menu and display Raw Data.
• MORE: Used to display additional functions (levels).
• PRINTER OUTPUT: Used to toggle printer output ON and OFF.
• HELP/ERROR: Used to display help information regarding the diagnostics menu screens. The fault log can also be
accessed through this key function.
• MAIN: Used to return to Main Menu.
Level Two
• WBC HISTOGRAM: Used to display WBC count and histogram data accumulated in each of 256 size channels.
• RBC HISTOGRAM: Used to display RBC histogram data accumulated in each of 256 size channels.
• PLT HISTOGRAM: Used to display PLT count and histogram data accumulated in each of 256 size channels.
• SMOOTHING ON/OFF: Used to toggle histogram display status. With Smoothing Off, only raw counts are displayed.
With Smoothing On, channels are numbered, data is normalized and the number of the peak channel displays.
Smoothing On/Off (example) gives an example of Smoothing On/Off.
• MORE: See Level One description.
• PRINTER OUTPUT: See Level One description.
• HELP/ERROR: See Level One description.
Level Three
• PROBE HOME: Moves Sample Probe up and above RBC Cup. Displays probe assembly sensor status.
• PROBE UP: Moves Sample Probe up. Displays probe assembly sensor status.
Level Four
• SYSTEM STATUS: Used to display all pending alarms.
• FAULT REPORT: Used to display all pending faults or warnings.
• SERVICE HEX CODES: Hex system codes. Not used for operator or service troubleshooting.
• SERVICE DEC CODE: Used to initiate individual actions in the CELL-DYN 1800 hardware and software.
Smoothing On/Off (example)
Fault Report Description
A detailed list of all faults generated by the CELL-DYN 1800 System software and hardware is contained in CELL-DYN 1800
Error Messages. The fault classifications reported in the Fault Report primarily contains data pertaining to the last CCM fault.
If a fault occurs, pressing the [HELP/ERROR] key immediately displays the [FAULT LOG] in the DIAGNOSTICS Menu. This
log may contain up to 16 faults, with the most current fault at the top of the list. An alternative procedure is to go to the MAIN
MENU and press [DIAGNOSTICS]. In this case, the [FAULT REPORT], not the Fault Log, is immediately displayed.
The Fault Log can be viewed from any of the menus, except SETUP. To view the Fault Log, enter the desired menu, followed
by [HELP/ERROR] and [FAULT LOG]. The system displays up to 16 past faults. From the MAIN MENU, press
[DIAGNOSTICS] followed by [MORE] three (3) times and [FAULT REPORT] to display the FAULT REPORT screen. A
display of <NO FAULTS OR WARNINGS PENDING> indicates that all faults have been cleared.
CELL-DYN 1800 Troubleshooting Guide
A list of symptoms, probable causes, and corrective actions for the most common problems encountered on the
CELL-DYN 1800 System is given in the Troubleshooting Chart. The probable causes and corrective actions for each
symptom are arranged in descending order from most likely to least likely. When troubleshooting a problem, start with the
most likely cause first.
If possible, thoroughly verify that a component is defective before replacement. Some problems can be verified visually, but
other problems may require a measurement tool such as a DVM (Digital Volt Meter).
When troubleshooting DATA PROBLEMS, only the measured parameters RBC, PLT, WBC, HGB, and MCV should be used
for reference. Using the calculated parameters can become confusing when trying to isolate a problem.
When troubleshooting CLOG AND FLOW ERROR PROBLEMS, refer to VP-04 Metering System Timing Adjustments - RBC
and WBC for the MIN and MAX specifications for the RBC and WBC Upper (T1) and Lower (T2) times.
Troubleshooting Charts
Nonfunctional Instrument Problems
Symptom Probable Cause Corrective Action
Power Cord Check Power Cord

No Functions. No Fans
Power Source Check Power Source
No 5VDC Check that 5VDC LED on Card Cage Backplane is On

Note
If off replace Power Supply Assembly.
No Functions. Fans Run
Defective CCM Replace CCM
Defective SBC Replace SBC
Video Display Problems

Loose Connection Check all LCD Display Screen Connections
Flash BIOS or Connect a computer monitor to the top of the SBC PCB. Power ON
CMOS settings instrument and open CMOS setup screen. Check that Advanced
LCD Display Screen reset to Default Chipset Setup is configured correctly. Refer to VP-05 CMOS Setup
Blank/Solenoids OK Verification/Adjustment.
Note
Defective Backlight Replace Backlight Interconnect PCB
Do not measure voltage
Interconnect PCB
on backlight inverter
PCB. Measuring voltage Defective LCD Replace LCD Interconnect PCB
can damage PCB. Interconnect PCB
Defective LCD Replace LCD Display Screen

Display Screen
Characters Out Of Focus Defective LCD Replace LCD Display Screen

Display Screen
Defective SBC Replace SBC PCB

PCB
Characters OK But Garbled
Display Screen
Defective SBC Replace SBC PCB

PCB
Missing Characters
Display Screen
Displayed Error and Fault Problems
Restriction Check Lines
Check In-Line Sensor

Detergent Empty Defective In-Line Sensor
Replace Sensor PCB
Defective CDM Replace CDM
Solenoid 3-1, 3-3, 3-4 Tubing Check Solenoid 3-1, 3-3, 3-4
Diluent Empty Restriction Check Lines
Check In-Line Sensor

Defective In-Line Sensor
Replace Sensor PCB
Check Solenoid 3-3

Solenoid 3-3
Replace Solenoid 3-3
Check Solenoid 3-4

Solenoid 3-4
Check Solenoid 3-1

Pressure Overlimit
Solenoid 3-1
Check Pressure Switch

Defective Pressure Switch
Replace Switch
Leak 8" Hg Check for air leaks in Fluid Power Supply and
Flow Panel
Vacuum Low Error Defective Pump Replace Pump
Defective Vac Regulator Replace Vacuum Regulator
Leak 0.5 Psi Check for air leaks in Fluid Power Supply and
Flow Panel
Pressure Low Error Defective Pump Replace Pump
Defective Pres Reg Replace Pressure Regulator
Check Unreg Pressure

Waste Overflow Into
Replace Unreg Pump
Accumulators No Air Pressure
Replace CDM
Check Solenoid 5-3

Solenoid 5-3
Check Solenoid 5-7

Solenoid 5-7
Check Solenoid 1-6

Solenoid 1-6
Check Accumulator Sensor Connections
Accumulator Sensor Falsely Detecting Flush Accumulator with DI Water

Liquid
Replace CDM
Check Internal Waste Sensor Connections

Defective Internal Waste Sensor
Replace Sensor
Check Unreg Pressure

Unreg Air Pressure Low
Waste Empty Timeout Replace Pump
Restriction Check tubing at Fluid Power Supply, Waste

Bottles, A and B
DLA/CCM Cable connection Check DLA/CCM Cable connection
Defective DLA/CCM Cable Replace DLA/CCM Cable
Defective CCM Board Replace CCM Board

CCM/DLA Communication Error
Defective DLA Board Replace DLA Board
Defective SBC PCB Replace SBC PCB
Power Source Check 5VDC and +12VDC (Digital) at Card Cage

Backplane PCB
Check CMOS Setup

Disk Errors (Hard Disk or Floppy
Defective SBC PCB
Drives) Replace SBC PCB
Defective Disk Drive (Hard Disk or Replace Disk Drive

Floppy Drives)
Defective Switch Replace Switch and Perform Alignment
Run Motor Power test

Defective Stepper Drive Printed
Replace Chopper Drive PCB
Circuit Board
Position Faults
Exercise Probe (Diagnostic Menu, Probe Home)
Defective Sample Probe Assembly
Replace Sample Probe Assembly
Run Motor Power Test

Defective Motor
Replace Motor
Data Problems
Check ±12VDC (Analog) LEDs on Card Cage Backplane

PCB
All Results Are "0" No ±12VDC (Analog) Replace Power Supply Module
Replace CCM
Check that PAM 100VDC LED is On

No 100VDC
Replace Power Supply Assembly
HGB OK All Others "0"
Defective SPM Replace SPM
Defective PAM Replace PAM
Reference lower than Check for bubbles in Detergent line and Solenoid 2-6
sample reading
No sample aspiration Check associated tubing, solenoids and sample syringe
Perform VP-08 Pre-Amplifier Module (PAM) Adjustment

HGB "0" all others OK
Defective PAM
Replace PAM
Defective HGB Flow Cell Replace HGB Flow Cell
Check 0.5 psi (Bubble Mix) pressure
0.5 psi (Bubble Mix) Check associated tubing and hardware

pressure
Perform Pressure Adjustment (0.5 psi)
Inadequate Probe Cleaning Check associated wash block tubing and hardware
Imprecision on all Parameters
Perform Sample Volume Verification
Imprecise Sample Aspiration
Check associated sample aspiration tubing and hardware
Imprecise Diluent Dispense Perform Diluent Volume Verification
Carryover Check for correct draining of Pre Mix Cup

Imprecision on all Parameters,
Defective PAM Replace PAM
HGB OK
Dirty RBC/PLT Transducer Clean RBC/PLT Transducer

Imprecision on RBC and PLT, Check 0.5 psi (Bubble Mix) Pressure
WBC/HGB OK
Incorrect 0.5 psi (Bubble Check associated tubing and hardware
Mix) Pressure
Perform Pressure Adjustment (0.5 psi)
Carryover Check for correct draining of RBC/PLT Transducer
Dirty WBC Transducer Clean WBC Transducer
Check 0.5 psi (Bubble Mix) Pressure
Incorrect 0.5 psi (Bubble Check associated tubing and hardware

Imprecision on WBC/HGB, Mix) Pressure
RBC and PLT OK Perform Pressure Adjustment (0.5 psi)
Carryover Check for correct draining of WBC Transducer
Dirty Flow Cell Clean Flow Cell
Check reference reading (Diagnostics Menu, Raw Data)

Reference reading out of
Perform HGB Ref Adjustment on PAM VP-08 Pre-Amplifier
Imprecision on HGB, Others OK specification Module (PAM) Adjustment
Defective Flow Cell Replace Flow Cell
Solenoid operation Check Solenoid 3-6, 2-6 and 2-7
Dirty Aperture. Clean RBC/PLT Transducer and Aperture Plate
RBC RER Perform VP-09 Signal Processor Module (SPM)

Imprecision on MCV Verification/Adjustment
Dirty Transducer Clean WBC Transducer and Aperture Plate
Replace Lyse Syringe

Incorrect Lyse Volume
Replace Lyse Syringe Drive Assembly
WBC "R" Codes, Reagents OK WBC Gain Perform VP-09 Signal Processor Module (SPM)
Verification/Adjustment
Replace SPM
Defective PCBs
Replace CCM
Check power
High Electrical Backgrounds Environmental
Check overhead lighting
Check for other items on same power line

Install filter (line conditioner)
Isolate line (dedicated line)
Check grounding cable on Front Cover
Poor instrument cover Replace grounding cable

grounding
Check EMI Shielding on Card Cage Backplane PCB
Check correct routing of WBC and RBC/PLT Transducer

cables to PAM
Defective PAM
Replace PAM
Check Power Supply Module

"Noisy" Power Supply
Replace Power Supply Module
Module
Check Connections on Pre-Amplifier Filter PCB

Defective Pre-Amplifier Filter
Replace Pre-Amplifier Filter PCB
PCB
Defective Transducer(s) Replace Transducer(s)
Clog and Flow Error Problems
Perform VP-03 Vacuum and Pressure Adjustments

Incorrect vacuum
Check Solenoid 1-4 and 1-5 (closed during metering)
Diluent and Detergent lines Check Reagent lines

"CLOG" both sides constant
reversed
Metering Tube position Top of Metering Tube (tapered edge) should be visible on
top of upper DET sensor
Check Vent Tubing

"CLOG" T1 = Max T2 = 0, No Vent Restriction
Check Vent Solenoid 3-6, 2-1 and 1-3
Dirty Transducer Clean Transducer and Aperture Plate

"CLOG" T1 = Max T2 = 0, No
Check plumbing
Meniscus
Restriction
Check Count Solenoid 1-2 and 4-3
Check Vent Solenoid 1-3 and 2-1

Check for bubbles on right side of Transducers
Dirty Transducer Clean Transducer and Aperture Plate
Check associated tubing
"CLOG" T1 = Max T2 = 0, Slow Restriction

Check Vent Solenoid 3-6
Meniscus
Perform VP-03 Vacuum and Pressure Adjustments
Incorrect Vacuum
Check Solenoid 1-4 and 1-5 (closed during metering)
Defective Upper Detector Replace Metering Printed Circuit Board

"CLOG" T1 = Max Meniscus
speed OK
Defective Lower Detector Replace Metering Printed Circuit Board

"CLOG" T1 =OK T2 = Max
Meniscus speed OK
Defective Upper Detector Replace Metering Printed Circuit Board

"FLOW ERR" T1 = Min T2 = Max
Defective Lower Detector Replace Metering Printed Circuit Board

"FLOW ERR" T1 = OK T2 = Min
Miscellaneous Problems

Garbled Histograms
Raw Data Description
From the MAIN MENU, press [DIAGNOSTICS] followed by [RAW DATA]. The [RAW DATA] key displays raw data obtained
from the last count cycle.
When a single count is done, all data is contained in the first column. When a PLT recount occurs, data from the first cycle
displays in column #2 and data from the recount displays in column #1.
Raw Data Display Description

• RBC, WBC and PLT counts are RAW, uncorrected total counts.
• HGB Error is not used.
• HGB Reference is the output of the A/D Converter when reading reference (2000 = 5 volts).
• HGB Sample is the output of the A/D Converter when reading sample (2000 = 5 volts).
• WBC and RBC Up Times are the upper times in milliseconds for the last sample.
• WBC and RBC Count Times are the times in milliseconds for the last sample.
• Flow Error is coded Clog or Flow Error data.
• RBC RER is RBC Cell Editing percentage.
• WBC and RBC Upper max and Upper min are the maximum and minimum Upper Times, respectively.
• WBC and RBC Avg. Time are the averages of the previous count times. The WBC and RBC Avg. time is reset when
the [Clear Orifice] key is pressed.
• WBC and RBC Time-Outs are the floating Upper Clog Alarm Limits calculated by the "Running Average Program".
Note
An example of a raw data report displays in Raw Data (example).
Raw Data (example)
CCM On-Board Diagnostic LEDs
The seven LEDs on the CELL-DYN 1800 CCM can reveal much about the fundamental CCM and overall machine state. In
general, the LEDs indicate whether the CCM is in a normal functioning mode or in a fault state, and in either case, help to
characterize the CCM state. Also, LED2 gives some information about the state of the SBC.
The CCM tests itself on power-up. These fundamental tests include MC68HC11KW1, RAM, and SPM interfaces. If any test
fails, the CCM attempts to execute an endless loop routine which flashes the green LED on the board. Also, it places a 4-bit
fault code into the adjacent yellow LEDs (see Power-on LED Patterns - Fault States on Startup).
LED Function & Counting Control

The LEDs on the CCM (labeled LED1 through LED7) are entirely under program control. Their use is as follows:
Note
Refer to CELL-DYN 1800 CCM for location of LEDs on PCB.
• LED7, a green LED, should not be not flashing after the CCM has successfully completed its internal power-on
self-check diagnostics, otherwise if it is flashing slowly (~1Hz.), there is a fundamental CCM fault.
• LED5 and LED6, for CER and CEW, indicate the state of the CCM firmware generated signals CER (Count Enable
Red) and CEW (Count Enable White). These signals enable cell counting. When the associated LED is on, the DMA
cell counting circuitry is active.
• LED3 and LED4, for WCP and RCP, are on during the metered count time intervals, LED3 for the white count time
interval and LED4 for the red count time interval.
• LED2 is driven by the signal NREQ1, and indicates the UIC/CCM communication activity. This LED state directly
relates to the hi/lo state of NREQ1. When LED2 is flickering, which should always be the case during normal system
operation, it indicates that the UIC/CCM communications link is active. (There is a corresponding LED on the DLA
(UIC comm. board) that will also be flickering in sync with LED2; it is controlled by the DLA output signal NREQ2.)
• LED1 is used to indicate that a self-test is in progress. This indicates that the tests for the pulse processing A to D
circuitry on the SPM with the pulse counting circuitry on the CCM are active.
Power-on LED Patterns - Normal Setup
LED2 LED3 LED4 LED5 LED6 LED7

Step State
NREQ1 WCP RCP CEW CER READY
0 ON ON ON ON Power on
1 RAM testing
2 Flashing RBC/PLT testing
3 Flashing WBC testing
4 ON ON CCM tests done; Homing flowscript running
5 Flashing ON ON UIC program comm. started
6 Flashing Initialized (running/idle)
Power-on LED Patterns - Fault States on Startup
LED2 LED3 LED4 LED5 LED6 LED7 CCM

NREQ1 WCP RCP CEW CER READY Fault
ON ON Flashing MC68HC11KW1 CONFIG reg
ON Flashing MC68HC11KW1 RAM test
ON Flashing 8K RAM bit test
Flashing 8K RAM clear test
ON ON ON Flashing Histogram RAM test
ON ON Flashing MC68HC11KW1 timer test
ON Flashing CDM init. test
CELL-DYN 1800 CCM

1 Cell Count Monitor (CCM) PCB 8 J6 - NOT USED
2 S1 RESET 9 LEDs 2-7
3 S3 BUFFALLO 10 E2 DGND
4 J2 MPM 11 J8 - NOT USED
5 J1 CDM 12 E3 DGND
6 J3 SPM 13 E1 DGND
7 J5 DLA 14 LED 1
CPU Hardware/Software Configuration
RS-232 Communications Test Procedure
Detailed information on the CELL-DYN 1800 System Interface Specifications is available on the WWCS Intranet website.
CMOS Setup
The CMOS Setup contains all the information needed by the Basic Input/Output System (BIOS) to establish proper
communications between the single board computer (SBC) and the various computer system devices. Refer to VP-05 CMOS
Setup Verification/Adjustment:
Special Function
Probe Check
There are two probe check functions activated by softkeys in the DIAGNOSTICS menu.
• The [PROBE UP] softkey moves the probe up and maintains position without rotational motion. (The [PROBE
DOWN] softkey then displays to restore the probe to the down position.)
• The [PROBE HOME] softkey places the probe over the RBC/PLT transducer. (The [PROBE DOWN] softkey then
displays to restore the probe to the down position.)
Note
Neither procedure puts the probe in the STANDBY position (on the left).
Service Special Commands
Discussion
Several commands are available to initiate individual actions in the CELL-DYN 1800 System hardware and software. These
commands are used for troubleshooting and/or alignment when a single action is desired or required to be repeated several
times.
The special command mode resides in the DIAGNOSTICS Menu. From the MAIN Menu, press [DIAGNOSTICS] followed by
[MORE] three (3) times, then [SERVICE DEC CODE]. When this softkey is pressed, the message Test Select --- FOR
SERVICE USE ONLY --- displays.
A command can now be entered. Pressing the Enter key on the keyboard initiates the action. Only one command can be
entered at a time and [SERVICE DEC CODE] must be pressed before a command is entered.
All commands available by direct softkey can be accessed by pressing [MORE].
Note
Use only the commands listed in DEC Service Commands and always verify that the correct number has been
entered before initiating the action. Use only those numbers listed in DEC Service Commands. Other numbers may
refer to engineering commands which are not used in the field and which may cause damage if used improperly. Be
fully aware of the purpose of any of the DEC Service Commands before using them. This is a direct-activation
method which should be used with caution because the physical state of the CELL-DYN 1800 System may not be in
agreement with the function to be performed. After using service commands, always re-initialize the system by
turning the power OFF then ON again or by pressing the [INITIALIZATION] key in the DIAGNOSTICS Menu to
ensure the instrument is in the proper configuration for normal operation.
DIAGNOSTICS Menu Service Code Function List
When the [SERVICE DEC CODE] key is pressed, the (Enter number (currently, 102):____ prompt displays.
The number above corresponds to the decimal code for the last code entered.
DEC Service Commands lists the decimal-coded (DEC) service commands that can be invoked by pressing the [SERVICE
DEC CODE] key in the DIAGNOSTICS Menu and entering the appropriate number.
DEC Service Commands
UIC DEC Codes Function
07 NOT USED
08 NOT USED
09 NOT USED
11 NOT USED
15 fill lyse into system
16 NOT USED
17 NOT USED
18 NOT USED
19 fill Diluent & detergent
20 mini-wash
22 NOT USED
23 NOT USED
24 NOT USED
25 NOT USED
26 NOT USED
33 NOT USED
34 NOT USED
36 NOT USED
37 pre-dilute sample run setup
38 pre-dilute sample run exit

39 aperture current off (uses whole blood script)
40 open all valves
41 NOT USED
47 platelet recount
48 initialization (homing)
49 open sample run
50 clean orifice (back-flushing)
51 pre-dilute sample run
52 background count run
53 prime system with all reagents
54 daily shutdown
55 empty transducers and cups
56 gain adjust
57 unpinching normally closed valves
59 fill transducers and cups after empty
60 gain adjustment setup
61 dispense 10 ml saline
62 open sample wash
63 clean-for-shipping
64 clean sample syringe setup
65 aspirate 40 µl sample for 1/250 dilution
66 dispense 10 ml saline for 1/250 dilution
67 aspirate 100 µl sample for 1/50 dilution
68 dispense 5 ml for 1/50 dilution
69 NOT USED
71 lyse syringe down
72 NOT USED
73 NOT USED
74 lyse syringe up and home
75 lyse syringe down restore

76 pre-dilute sample wash
77 NOT USED
78 NOT USED
81 NOT USED
83 diluent syringe down
84 enzyme clean setup
85 probe up and rotate and home
86 back to ready position from probe home
87 probe up for probe adjustment
88 probe down (when finished, operator should initialize the instrument to place the probe in the home
position)
89 sample syringe up and restore
90 sample syringe down and home
91 enzyme clean the system
92 diluent syringe up and home
93 diluent syringe down and restore
117 NOT USED
118 NOT USED
119 NOT USED
120 NOT USED
121 cycle solenoids on waste assy
122 cycle solenoids on flow panel assy
123 sample syringe aspirate
124 sample syringe dispense
125 vacuum test
126 check mixing pressure
127 check backflush pump
128 motor power test (see Service DEC Code 128)
129 motor power level test (see Service DEC Code 129)
130 exercise motors (see Service DEC Code 130)

999 auto-cycle (see Auto-Cycling (Code 999))
Note
Certain commands are not sent to the CCM when the system is in an interlock state, such as STANDBY or
UNINITIALIZED.
Auto-Cycling (Code 999)
The CELL-DYN 1800 can be pre-set to do a specified number of RUN cycles without user intervention. This capability
applies only to normal RUN Count Test, Pre-Dilute RUN, (PRE-DIL TEST), Gain Adjust (GAIN ADJ), and Electrical
Background (ELEC BKGD). This capability helps reduce test time for the instrument. The following entry screen displays
after entering code 999:
-- Auto Cycle Test Set Up --
Use Spacebar to accept current number
Use "<--" key to delete a digit
Use "ESC" key to cancel
Enter Number of Times to Repeat Test (currently, 10):
Sample Probe Description
The motors that enable the Sample Probe to move up/down and to rotate are stepper motors which are under direct
computer control. Since there is no direct positional feedback sent to the computer, position switches are employed to verify
critical positions during normal operation. It is important to understand that these switches only verify and do not control the
movement of the Sample Probe.
In the DIAGNOSTICS Menu, Service DEC Codes 128, 129, and 130 allow the service representative to control and exercise
all stepper motors in the CELL-DYN 1800 System. This description focuses on the Probe Up/Down Motor (B/2) and the
Probe Rotate Motor (C/3) which control the movement of the Sample Probe.
Service DEC Codes 128, 129, and 130 Descriptions
These commands reside in the SERVICE DEC CODE screen of the DIAGNOSTICS Menu and are used to test, control, and
exercise CELL-DYN 1800 stepper motors. A description of each of these three commands is given.
Service DEC Code 128

This code runs a computer generated test (Motor Power Test) of all stepper motors, motor driver boards, and associated
circuitry.
The Motor Power Test should be run whenever a problem is suspected with any assembly that is driven by a stepper motor.
The following entry screen displays after entering code 128:
Motor Power Test Started.
To MPM: {I }
To MPM: {pD32}
To MPM: {mC1!2} AC}
To MPM: {C1}
inp: 0415
A report (Motor Power Test (example)) automatically displays and can be printed. Refer to VP-11 Stepper Motor Power Test
and Verification.
Note
Press the [INITIALIZE] key before leaving the DIAGNOSTICS Menu.
Motor Power Test (example)

This code allows the Run and Idle power levels to be set when exercising a stepper motor. The four levels are:
0) Full Power
1) Medium Power
2) Low Power
3) Off
This code tests mechanical assemblies at various power levels or to remove idle power so the mechanism can be more
easily moved or checked manually. The Motor Power Level Test (example) screen displays after entering code 129 (press
the ENTER key after each entry):
Motor Power Level Test (example)
Note
After the entries are made, a message, such as Motor "A" set to running power of 1 and idle power of 3,
displays.
This code allows the direction, speed, and number of steps to be set when exercising a stepper motor.
The Motor Check (example) screen displays after entering code 130:
Motor Check (example)

Note
After the entries are made, a message, such as Motor "B": motion in direction "0" at speed "&" for 100 steps,
displays.
Motor Direction Commands
The table below contains information on the motor designation, command and direction of the motor to be tested. Motor
Speed Commands lists the motor speed commands to determine the speed of the motor being tested. Both tables are
needed to properly test the motor.
Motor Direction Commands
Motor Designations Function Command Direction
0 Down/Aspirate
A/1 Sample Syringe
1 Up/Dispense
0 Up
B/2 Probe Up/Down
1 Down
0 CCW/To RBC cup

C/3 Probe Rotation
1 CW/To Pre-Mixing Cup
0 Down/Aspirate
D/4 Diluent Syringe
1 Up/Dispense
0 CCW/Dispense
E/5 Directional Valve 1 CW/Aspirate
F/6 Spare
G/7 Spare
0 Down/Aspirate
H/8 Lyse Syringe
1 Up/Dispense
Motor Speed Commands
Command Speed in Steps per Second
1 50
2 75
3 283
4 300
5 166
6 200
7 250
8 10
9 151
10 222
11 25
12 182
13 100
14 125
15 91
16 67
17 111
Sample Probe Normal Operation

Probe Up/Down INITILIZE and RUN Modes illustrates the Sample Probe's up/down sequence during the INITIALIZE and
RUN cycles. Probe Rotate "INITALIZE" Mode shows the probe's rotation movement during the INITIALIZE cycle.
Probe Up/Down INITILIZE and RUN Modes
Probe Rotate "INITALIZE" Mode
Initialization Mode
The Initialization cycle places mechanical and electrical components in the "home" position, drains any liquid in the tubing,
Pre-Mix Cup, and the Mixing Chamber of the von Behrens RBC Transducer to the Waste System, then places the instrument
in the INITIALIZED state.
Stepper Motor Homing
Homing a stepper motor is the process of setting up the initial position from which all future movement is referenced. In the
CELL-DYN 1800 System, this is accomplished by forcing the motor to move against a physical stop (Hard Stop). When the
mechanical assembly, driven by the motor, reaches the Hard Stop, the stepper motor electrically slips until it is forced to stop.
This mechanical position then becomes the zero reference position for the motor.
Operation:
1. The Sample Probe moves up at a fast speed until the Upper Switch (#2) is activated. It is then changed to a slow
speed, and homed against the Upper Hard Stop, which is the metal plate at the top of the Sample Probe Assembly.
2. The probe moves down six steps and the Upper Switch (#2) is checked.
3. The probe moves CCW at a fast speed until the Right Switch (#4) is activated. It is then changed to a slow speed,
and homed against the Right Hard Stop, which is the mounting bracket for Right Switch (#4).
4. The probe moves CW to the Pre-Mix Cup and Left Switch (#3) is checked. The probe then moves into the Pre-Mixing
Cup.
5. The probe moves up and Upper Switch (#2) is checked.
6. The probe moves CCW to center and down positions; and the Lower Switch (#1) is checked.
7. This completes the Initialization cycle.
Run Mode
The figure below illustrates the probe's movements during the RUN cycle.
Probe Rotate "RUN" Mode

Operation:
1. When the Start Switch is pressed, 30 µL of sample is aspirated and Lower Switch (#1) is checked.
2. The Sample Probe then moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.
3. The probe moves CW to Pre-Mix Cup and Left Switch (#3) is checked.
4. The probe moves down eight steps and into the Pre-Mix Cup, where dispense, probe shake, and aspiration of RBC
sample takes place.
5. The probe then moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.
6. The probe moves CCW to the Mixing Chamber of the von Behrens RBC/PLT Transducer, stops three steps from
Right Hard Stop, and Right Switch (#4) is checked.
7. The probe moves down into the RBC/PLT Mixing Chamber and RBC sample is dispensed.
8. The probe moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.
9. After completion of the count cycle, the probe moves CW to center position.
10. The probe moves down and Lower Switch (#1) is checked.
11.This completes the RUN cycle.
Switch Failure Descriptions
Example of fault reports are shown in the following figures:
Lower Switch (#1) Fault Report
Upper Switch (#2) Fault Report
Left Switch (#3) Fault Report
Right Switch (#4) Fault Report

When a switch is checked by the computer and found to be deactivated (open) in normal operation, the message "Not Ready:
SEE DIAGNOSTICS" displays on the RUN Menu.
From the MAIN MENU, press [DIAGNOSTICS]. The screen immediately displays one of the Fault Reports shown in Lower
Switch (#1) Fault Report, Upper Switch (#2) Fault Report, Left Switch (#3) Fault Report, and Right Switch (#4) Fault Report.
The message <SWITCH: 1 CHECK> indicates that Lower Switch (#1) failed when checked. The message <* NOT ON ANY
SWITCH *> indicates that none of the switches were activated when the failure occurred. Refer to Lower Switch (#1) Fault
Report.
Lower Switch (#1) Fault Report
The message <SWITCH: 2 CHECK> indicates that Upper Switch (#2) failed when checked. The message <* NOT ON ANY
SWITCH *> indicates that none of the switches were activated when the failure occurred. Refer to Upper Switch (#2) Fault
Report.
Upper Switch (#2) Fault Report
The message <SWITCH: 3 CHECK> indicates that Left Switch (#3) failed when checked. The message <ON SWITCH(ES):
2 TOP> indicates that Left Switch (#3) was activated when the failure occurred. Refer to Left Switch (#3) Fault Report.
Left Switch (#3) Fault Report
The message <SWITCH: 4 CHECK> indicates that Right Switch (#4) failed when checked. The message <ON SWITCH(ES):
2 TOP> indicates Right Switch (#4) was activated when the failure occurred. Refer to Right Switch (#4) Fault Report.
Right Switch (#4) Fault Report
The above conditions do not necessarily indicate that a switch has actually failed. They only indicate that the switch was not
read as activated when checked by the computer. A failure could also be caused by improper switch alignment, an electronic
hardware failure, or a mechanical hardware failure.
CELL-DYN 1800 Error Messages
The table below lists error messages on the CELL-DYN 1800 System.
Error Message Description
(Status box)
Time-out at N seconds A CCM process initiated by the user took longer to complete than allowed (usually indicating
a failure of the CCM). The process ran approximately N seconds before the time-out
occurred.
Process Aborted A count test was stopped either by the user or because of a fault detected by the CCM.
Fix then press [CLEAR A user-correctable fault condition was detected.

ALARM]
Process Monitoring Aborted A process was stopped by the user (using the asterisk (*) key.
Error Message Description of the error.

(Display Area II)
Printer Time-out The Printer Output option was ON and the printer did not print the requested report in the
expected time.
Code N is Invalid The user has entered a command for the CCM whose numeric value exceeds 127. The
value entered was N.
Cannot do this Function The user has attempted to issue a command to the CCM that cannot be executed because
of a pending fault condition.
WBC Meniscus Detection, During the most recent count, a meniscus was not detected or was detected at an
RBC Meniscus Detection unexpected time.
WBC Count Time-out (clog), During the most recent count, a Clog occurred.
RBC Count Time-out (clog)
CCM Pulse Height Memory During the most recent count, there was an overflow in one of the pulse-height arrays
Saturation Warning (histograms).
External Waste Full The external waste bottle has been filled.
Lyse Empty, Detergent The indicated reagent has run out.

Empty, Diluent Empty
Invalid Alarm Set A bit was set in the fault message from the CCM that has no valid interpretation.
*NOT ON ANY SWITCH* After some mechanical motion, a reading of all the position sensors indicates that none are
activated. (This message does not necessarily mean that a mechanical fault has occurred.)
Waste Overflow Into A reading of the sensor in the Waste Accumulator suggests that there is liquid in the
Accumulators accumulator.
Error Message (Status box) Description
Vacuum There was a vacuum failure during power-up or the instrument is unable to maintain vacuum
level while in the READY state.
Pressure There was a pressure failure during power-up.
Position Fault A mechanical assembly is not in the correct position for the most recent function to be
performed, as indicated by position sensors.
Sensor Fault - Internal Waste A Time-Out fault occurred in draining one of the waste bottles. This error is also associated
Empty with positive pressure.
Canceling Auto-Cycling This message displays when the user cancels Auto-Count Testing.
Invalid UIC Command Sent to The UIC sent a command to the CCM that it cannot interpret.
CCM
Error in Flow System Timing An error in the timing of a flow script has occurred. This occurs during instrument
initialization when the flow script takes more than three minutes to complete.
Histogram Memory Clear The CCM was unable to clear the pulse-height memory.
CCM Program, RAM Memory The CCM detected a failure in its RAM.
CCM/MPM Message Fault
Other error-related messages
MPM to CCM, Message

Transmit Error
Command to be Sent to MPM

is Incorrect An error in CCM/MPM interprocessor communications occurred. A fault was generated in an
Abnormal Time-out/No MPM attempt to send or receive motor or other command to or from MPM, or the MPM was unable
Response to perform the function.
Fault Response from MPM
Incorrect Command to be
Sent to MPM
Attempt to Send MPM a New

Command while Busy
No Such Script in ROM or Error in loading a flow script.

RAM
CCM/UIC Message Fault An error in UIC/CCM interprocessor communications occurred.
No Response from CCM The CCM is not functioning or the signal cable connecting the CCM and UIC is faulty or
disconnected. Turn the instrument OFF, check the CCM/UIC cable, then turn the instrument
ON.
CCM is Initializing The CCM is in the middle of its Initialization process.
Undefined Event An undefined event or process occurred.
Count Test
The [COUNT TEST] key in the DIAGNOSTICS Menu is used to run specimens and display Count Check data without
returning to the RUN Menu. Coded data relating to specific cycle functions, raw measurement, and flow count time are
displayed for use in troubleshooting or service.
Event Messages During Diagnostic Menu Count Test lists the event messages that are displayed during the Diagnostic Menu
Count Test.
Event Messages During Diagnostic Menu Count Test
Event Messages Description

SampSw. pressed Touch Plate was pressed.
Remove specimen Specimen should be removed.
Count valve open The counting valve is open.
RBC histogram avail RBC histogram is available.
WBC upper det WBC upper meniscus detection.
WBC lower det WBC lower meniscus detection.
RBC upper det RBC upper meniscus detection.
RBC lower det RBC lower meniscus detection.
Plt recount strt Platelet recount starts.
CCM initing CCM initializing.
Data invalid Data entered is invalid.
WBC histo avail WBC histogram is available.
Proc complete Process is completed.
Data avail Data is available.
CCM init done CCM initialization is completed.
Canceled Canceled operation.
Operator-Correctable Alarm or Fault Messages
This table lists operator-correctable alarm or fault messages.
Event Messages Description
External Waste Full Waste full sensor is activated.
Detergent Low Detergent is low as detected by reagent sensor in reagent inlet tube.
Diluent Low Diluent is low as detected by reagent sensor in reagent inlet tube.
Lyse Empty No lyse is detected by reagent sensor in reagent inlet tube.
Invalid alarm set Incorrect error or message was set.
Detergent Empty No detergent is detected by reagent sensor in reagent inlet tube.
Diluent Empty No diluent is detected by reagent sensor in reagent inlet tube.

CCM is initializing CCM module is currently in the INITIALIZED state.
Summary of Error Messages
Summary of Error Messages gives a complete listing of error messages available on the CELL-DYN 1800 System.
Summary of Error Messages
Error Message (Status box)
DOS Errors
Arithmetic overflow
Bad drive request structure length
Cannot remove current directory
Cannot rename across drives
Collection index out of range
Collection overflow
CRC error in data
Device read fault
Device write fault
Disk full
Disk is write-protected
Disk read
Disk seek
Division by zero
Drive not ready
File access denied
File not assigned
File not found
File not open

File not open for input
File not open for output
Floating point overflow
Floating point underflow
Hardware failure
Heap overflow
Invalid drive number
Invalid file access code
Invalid file handle
Invalid floating point operation
Invalid function number
Invalid numeric format
Invalid pointer operation
Path not found
Printer out of paper
Range check
Sector not found
Stack overflow
Too many open files
Range check
Sector not found
Stack overflow
Too many open files
Unknown command
Unknown media type
Unknown unit
General
Demographic entry disabled
No data to print
Stopping
Unable to Load Flowscript
Unable to Save Cal Factors
Unable to Set Volume
System Fault
Not Ready: See DIAGNOSTICS
Uninitialized
Normal Operator-Correctable
Detergent empty
Diluent empty
Lyse empty
Waste full
DLA Faults
<-? Invalid error code
CCM command echoed does not match
CCM REQ1 high during data send at char
CCM REQ1 is stuck LOW
DLA buffer overflow on receiving char
DLA/CCM error on command
Incorrect checksum on CCM data receive
NAK received from CCM
No response from CCM
Time-out 1 on wait for CCM REQ1
Time-out 2 on message send to CCM
Time-out 3 on message receive from CCM
Date
Battery failure or system date/time not set
Disk I/O
Cannot open CD1800.ini configuration file
Cannot read CD1800.ini configuration file
Cannot write to CD1800.ini configuration file
CD1800.ini configuration file size error
CD1800.ini configuration file version error
Configuration file error
Error when reading CRC values from disk
Memory error when creating CRC tables
Ticket Printer
Data err
Inc. asp.
No Ticket Detected
QC Log
Can not accept specimen
Can not reject specimen
No loading, QC file has to be empty
Purge log failed
Read QC file failed
Data Log
Cannot do if uninitialized
Cannot write Data Log header to disk
Count overrange
Data Log write error
Failed to read from Data Log

Flow err
Initialize
Data Log initialization failed
QC Log initialization failed
Communications
Break interrupt
Comm open error
Comm error
Framing error
Parity error
Re-transmit started
Re-transmit time-out
Receiver overrun
Transmit started
Unable to re-transmit
Printer Driver
Printer Fault
Printer Not Ready
Printer Off-line
Printer Out Of Paper
Printer Time-out
Ticket Printer Not Ready
QC Log I/O
Failure to read from QC Log
Failure to write to QC Log
QC Log size error

QC Log version error, bytes short for creation of QC Log
Help
Unable to open help file
Reagent Log
Unable to create file
Unable to open file
Data Log Errors
Cannot Access Data Log
CRC Reading Failed
Data Log initialize failed, bytes short for creation of Data Log
Data Log Print Error
Data Log size error, Call Technical Support
Data Log version error
Data Transmission Error
Not enough memory error
X-B File
Failed to write to Data Log Header
Failed to write to Data Log
No response from CCM
Data Log I/O
Data Log write failure
Write Header failure
Write version error
General Faults
Abnormal time-out / no MPM response
Attempt to send MPM a new command while busy
CCM pulse height memory saturation warning
CCM is in Fault State
CCM is in Unknown State
CCM Program, RAM Memory Fault
CCM real time clock has failed
CCM/DLA Message Communication Fault
Command to be sent to MPM is incorrect
Count Overrange
Count time-out (clog)
Data Capture Fault
Detected Probe Assembly Switch: # in Incorrect State
Detergent sensor
Diluent sensor
Error in Flow System Timing
Fault response from MPM
Guard electrode voltage warning
Histogram Memory Clear Failure
Incomplete Aspiration
Incorrect command to be sent to MPM
Initial Communication with DLA Failed
Invalid Command Sent to CCM
Invalid Data
Mechanical Position Fault
MPM to CCM, message transmit error
No response from MPM
Press [INITIALIZATION] key to clear fault and re-initialize meniscus detector: "True" sensed at metering start meniscus: not
detected during valid time interval
Pressure Level Time-out

Pressure Over-Limit Detected
Printer is not Ready: Cannot RUN uninitialized
RBC Clog
RBC Flow Error
Run time error: # at:
Time out after approximately __ minutes
Turn off instrument and drain accumulators manually
Unexpected response from MPM
Unknown MPM/CCM fault
Vacuum Level Time-out
Waste Drain to Empty Time-out
Waste Overflow into accumulators
WBC Clog
WBC Flow Error
Wrong software for this instrument
Fault Log
Error in writing to Fault Log file
Error in writing header to Fault Log file
Fault Log I/O
Allocating heap memory error
Bytes short to create the Fault Log
Fault Log file size error
Fault Log Header IO Error
Incorrect version of Fault Log
Lab ID Setup
Drive A is not ready
Fail to read assay file

Fail to write LAB ID file
Incorrect assay format
Incorrect disk. QC values do not apply to this instrument
Incorrect Exp. Date
Incorrect Lot Number
Incorrect parameter in assay file
Software Commands and Sequence
CD1800 File and Directory Structure
Accessing DOS
There are two ways to access the DOS program from the CD1800 program:
• With the CD1800 program still loaded,

• By exiting the CD1800 to DOS. The Exiting from CD1800 to DOS method is the preferred method.
Switching from CD1800 to DOS
1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.

2. Press ESC on the PC keyboard. The following message displays on the screen.
CD1800 Program Exit
It is recommended that the Cell-Dyn be put in Stand-by before exiting the program. Otherwise, turn the
instrument off after doing any service work while in DOS.
3. Press the Alt and d keys simultaneously. The DOS prompt C:\CD1800> displays.
4. Execute the desired DOS commands. Most commands can be executed. However, computer memory is limited
since the CD1800 program is still loaded.
Exiting from CD1800 to DOS
1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.

2. Press ESC on the PC keyboard. The following message displays on the screen.
CD1800 Program Exit
It is recommended that the Cell-Dyn be put in Stand-by before exiting the program. Otherwise, turn the
instrument off after doing any service work while in DOS.
3. Press the Alt and x keys simultaneously. The CD1800 program is removed from computer memory. The DOS
prompt (C:\1800>) displays.
4. Execute the desired DOS commands.
Exiting DOS
To exit the DOS program and return to the CD1800 program in the case where the CD1800 program remains loaded
(Alt and d was used to access DOS), type exit at the C:\> prompt and press the Enter key. The DIAGNOSTICS Menu
displays.
To exit the DOS program in the case where the CD1800 program is not loaded, (Alt and x was used to exit the CD1800
program), turn the instrument OFF then ON again to reinitialize the system.
Note
Turning the instrument OFF then ON again prepares the system for normal operation.
Common DOS Commands
Function Command Description
Change drives c: or a: switch C and A drives
Change directory cd filename
Copy copy source to destination

Delete del file name(s)
Directory dir current or specified drive
Exit exit quits the current program
Undelete undelete file name
DOS Command Usage
Change Drives
When accessing DOS from the CD1800 program, the C:\> prompt displays, indicating the C drive is selected. To change to
the A drive (floppy disk), type A: and press Enter. The prompt changes to A:\> indicating the A drive is selected.
Change Directories/Files Within a Drive
To move from a directory to the root directory (C:\) type cd\ and press the Enter key.
To move one level closer to the root directory type cd.. (where .. are two periods) and press the Enter key. For example, to
move from CD1800\HELP to CD1800 subdirectory type cd.. and press the Enter key.
To move one level away from the root directory type cd_[directory name] (where _ indicates a space) and press the Enter
key. For example, to move from the root directory (c:\) to the CD1800 directory type cd cd1800 and press the Enter key.
Copy
To copy a file on the CELL-DYN 1800 hard drive (C drive) to a floppy disk (on the A drive):
1. Exit the CD1800 program procedure above. Change to A drive, if the file to be copied is on A drive.
2. If necessary, use the cd command to access the directory containing the file(s) to be copied.
3. At the C:\> prompt, type Copy_C:\[filename]_A:\ (where [filename] is the complete file name and _ indicates a
space between characters).
Example: to copy file CONFIG.SYS from the C drive to the A drive, type copy c:\config.sys a:\ and press the Enter
key.
To copy this file from the A drive to the C drive, type copy a:\config.sys c:\ and press the Enter key.
Delete a File
To delete a file:
1. Access DOS (Exiting from CD1800 to DOS). Change to A drive if the file to be deleted is on A drive.
2. If necessary, use the cd command to access the directory containing the file(s) to be deleted.
3. At the C:\> prompt, type delete_ [filename] (where [filename] is the complete file name and _ indicates a space
between characters) and press the Enter key. For example, to delete a file called Sample from the C drive type
delete sample and press the Enter key.
Undelete a File
To undelete a file which had previously been deleted:
1. At the C:\> prompt, type undelete_ [filename] (where [filename] is the complete file name and _ indicates a space
between characters) and press the Enter key. For example, to undelete a file called Sample from the C drive, type
undelete sample and press the Enter key.
View Files in a Directory
To view all the files in a particular directory:
1. Access DOS (Exiting from CD1800 to DOS). Change to A drive if the files to be viewed are on A drive.
2. If necessary, use the cd command to access the directory containing the file(s) to be viewed.
3. At the C:\> prompt type dir and press the Enter key.
Note
If there are many files, the file names scroll down the screen too rapidly for the user to see. The user can prevent this
by adding "switches" to the DIR command using the format: DIR_[switch] (where the underscore ("_") indicates a
space). For example, to display the file names a screen at a time, type dir_/p and press the Enter key. To display the
file names in columns across the width of the page: type dir_/w and press the Enter key.
Engineering Drawings and Schematics
见 CD1800 电路图
CELL-DYN 1800 PCB Reference
AC Regulator PCB
1 AC Regulator PCB 14 J1
2 J2 15 J3
3 TP6 16 J8
4 TP7 (+14V) 17 LD3 (+14V)
5 TP5 (VR) 18 LD4 (+5V)
6 TP4 (GND) 19 J5
7 R22 20 LD1 (+12V)
8 J4 21 LD2 (-12V)
9 J7 22 TP2 (GND)
10 TP9 (GND) 23 TP3 (+12V)
11 TP8 (+100V) 24 TP1 (-12V)
12 R28 25 J6
13 J9
Backlight PCB
1 Backlight PCB 3 CN2
2 CN1
Backplane PCB (front)
1 Backplane PCB 7 LD4 (+12V Digital)
2 J2 8 LD5 (-12V Digital)
3 SBC 9 LD2 (+12V Analog)
4 DLA 10 LD3 (-12V Analog)

5 CCM 11 LD1 (+5V)
6 SPM
Backplane PCB (rear)
1 Backplane PCB 7 TP3 (-12V Analog)
2 J1 8 TP2 (+12V Analog)
3 TP6 (DGND) 9 J10
4 TP1 (+5V) 10 J9
5 TP5 (-12V Digital) 11 J11
6 TP4 (+12V Digital)
CCM PCB
1 CCM PCB 8 J6
2 S1 (RESET) 9 LEDs 2-7
3 S3 (BUFALLO) 10 E2 (DGND)
4 J2 (MPM) 11 J8
5 J1 (CDM) 12 E3 (DGND)
6 J3 (SPM) 13 E1 (DGND)
7 J5 (DLA) 14 LED1
CDM PCB
1 CDM PCB 18 J25
2 TP1 (GND) 19 J26
3 J11 20 J27
4 J12 21 J28
5 J13 22 J29
6 J14 23 J30
7 J15 24 J31
8 J16 25 J10
9 J17 26 J9
10 J18 27 J8
11 J19 28 J7
12 J32 29 J6
13 J20 30 J5
14 J21 31 J4
15 J22 32 J3
16 J23 33 J2
17 J24 34 J1
DC Regulator PCB
1 DC Regulator PCB 9 J1
2 LD2 (+14V) 10 J11
3 J5 11 LD3 (+12V)
4 J7 12 J8
5 J6 13 J9
6 J4 14 J10
7 J2 15 LD1 (+5V)
8 J3
DLA PCB
1 DLA PCB 5 DS2 LED
2 J1 6 J2
3 TP1 7 TP2
4 DS1 LED
Indicator PCB
1 Indicator PCB 3 LD2 (yellow)
2 LD1 (green) 4 LD3 (red)
Internal Waste Empty PCB
1 Internal Waste Empty PCB 4 J1
2 E2 5 J2
3 E1
LCD Interconnect PCB

1 LCD Interconnect PCB 6 J2
2 J8 7 J3
3 J7 8 JP1B
4 J5 9 JP1A
5 J6 10 J4 (USED FOR CELL-DYN 1800)
MPM PCB
1 MPM PCB 7 TP4 (+28V)

2 DS1 thru DS4 LED'S 8 TP2 (GND)
3 TP1 (GND) 9 TP6 (+12V)
4 TP3 (RESET) 10 J2
5 J3 thru J14 11 J1
6 TP7 (+5V)
PAM PCB
1 PAM PCB 13 TP3 (GND)
2 J3 14 JMP3
3 J4 15 R14
4 JMP1 16 J5
5 J1 17 R72
6 TP7 18 LD3
7 R4 19 TP5 (GND)
8 TP1 20 R66
9 JMP2 21 TP6 (GND)
10 TP2 22 LD1 (-12V)
11 J2 23 LD2 (+12V)
12 TP8 (GND) 24 TP4 (+100V)
Pre-Amp Filter PCB
1 Pre-Amp Filter PCB 4 -12V

2 +12V 5 J1
3 GND
PRM PCB
1 PRM PCB 6 J1
2 J5 7 J2
3 J8 8 J3
4 J7 9 J4
5 J6 10 F1
RBC Metering PCB

1 RBC Metering PCB 4 TP2
2 J1 5 TP3
3 TP1
Reagent Empty Sensor PCB

1 Reagent Empty Sensor PCB 5 J1
2 E1 6 E6
3 E2 7 E5
4 E3 8 E4
SBC PCB
1 SBC PCB 6 LPT1
2 Fan 7 COM1
3 Power 8 COM2
4 Hard Disk Drive 9 PC Keyboard
5 Floppy Disk Drive 10 LCD Display Screen
SDM PCB
1 SDM PCB 5 DS1 thru DS8 LEDs
2 TP2 (DGND) 6 J1 thru J8
3 J9 7 J10
4 TP1 PWR GND
SPM PCB
1 SPM PCB 6 E2 (AGND)
2 R22 (WBC) 7 J1
3 R27 (RBC) 8 J4
4 R41 (PLT) 9 E1 (DGND)
5 R51 (RER) 10 E3 (AGND)
Touchpad PCB
1 Touchpad PCB 3 J2
2 J1
Vacuum/Pressure Regulator PCB (front)
1 Vacuum /Pressure Regulator PCB 6 J1

2 P2 7 DS1
3 P1 8 R18
4 TP3 9 TP2
5 TP1 10 E1
Vacuum/Pressure Regulator PCB (rear)
1 Vacuum /Pressure Regulator PCB 2 R16
WBC Metering PCB

1 WBC Metering PCB 4 J1
2 TP1 5 TP3 (GND)
3 TP2
Removal & Replacement
Service Equipment Required
Every system subassembly and module can be removed and replaced using the tools listed below. Substitute equipment
having the same characteristics as those listed can also be used. Whenever possible, choose non-magnetic tools to avoid
damaging sensitive printed circuit board mounted components and magnetic media.
Required Tool Recommended Model Description
Cable Ties Self-locking
Flashlight Pen light
Knife Utility
Nut driver 1/4" 8-inch
Nut driver 1/2" 8-inch
Pliers 5" Diagonal Cut
Pliers 8" Long Nose
Screwdriver Phillips No. 2 18-inch
Screwdriver Phillips No. 1 Stubby
Screwdriver Phillips No. 2 Stubby
Screwdriver Slotted 3/16 X 10 8-inch
Screwdriver Slotted 3/16 X 8 8-inch
Screwdriver Hex 7/64" 8-inch
Spare tubing Silicone Various lengths
Wrench Adjustable
Covers (CD18-A1)
Removal
Action Steps Reference
Remove 1. Grasp under the lower right side [2] and

Upper Front open the cover [1] toward the left.
Cover
Note
Cover is hinged on the left side and is
kept closed with a magnetic latch.
2. Disconnect cable connector J1 [3] on

the Status PCB [4].
3. Disconnect the ground wire [5] from the
instrument chassis.
4. Use a slotted screwdriver to remove the
C clip [6] from the lower hinge.
Note
Be careful when removing the C clip.
The hinge contains a plastic washer [7]
and a spring [8] which can be lost.
5. Remove the plastic washer and spring.

6. Lift the cover from the hinge pegs and
remove it from the instrument.
Replacement
Install Upper Front Cover 1. Install in reverse order of disassembly.

Verification
Verification of Proper Cover 1. Place instrument into READY mode.

Installation 2. From the MAIN Menu, press RUN followed by SPECIMEN TYPE, then
NORMAL BACKGRND.
3. Press the touch plate to run a cycle.
Note
During the cycle, make sure that cover does not interfere with
instrument operation.
4. Once cycle is complete, verify acceptable background results. Refer to

Specifications & Checklist.
Removal
Open Upper 1. Open the upper front cover.

Front Cover
Check Caution
Sample Be sure the sample probe is out of the
Probe way before attempting to remove the
Position lower front cover to prevent damage to
the probe.
• If the instrument is ON, from the MAIN

MENU press [SPECIAL PROTOCOLS]
then [MORE] followed by [PROBE
HOME] to place the probe over the
RBC/PLT Mixing Chamber.
• If the instrument is OFF, make sure the
Sample Probe is raised all the way (if not,
turn the lead screw to raise the probe) so
that it clears the lower front panel.
Remove 1. Remove the thumbscrew [2] from the

Lower Front lower front cover (to the bezel).
Cover 2. Slide the lower front cover [1] to the left
approximately 1/2-inch until it clears the
bezel, then lift up and out.
Note
It may be necessary to lift the right side of
the cover to clear the bezel indentation
while sliding the cover to the left.
3. Verify that the upper left hand corner of

the cover clears the clip. [3]
Replacement
Install Lower Front Cover 1. Install in reverse order of disassembly.
Verification
Verification of Proper Cover 1. Place instrument into READY mode.

Installation 2. From the MAIN Menu, press RUN followed by SPECIMEN TYPE, then
NORMAL BACKGRND.
3. Press the touch plate to run a cycle.
Note
During the cycle, make sure that cover does not interfere with
instrument operation.
4. Once cycle is complete, verify acceptable background results. Refer to

Specifications & Checklist.
Removal
Remove 1. Using a Phillips-head screwdriver, remove

Top Cover the two (2) mounting screws [2] on the rear
of the instrument securing the top cover to
the chassis.
2. Lift and remove the top cover [1] by sliding
it to the rear.
Replacement
Install Top Cover 1. Install in reverse order of disassembly.
Verification
Verification of 1. Verify that cutouts on the front

Proper Cover portion of the top cover [1] are
Installation placed under the locking nuts [2].
2. Be sure that rear of the cover is
flush against the rear instrument
panel.
Removal
Open Upper 1. Open the upper front cover to the left.

Front Cover
Remove 1. Remove Lower Front Cover (RR-A1.02

Lower Front Lower Front Cover).
Cover

Right Bezel the left screw [2] on the right side
Screw cover [3].
Note
The Phillips screw secures the bezel to
the instrument.
Remove 1. Lift the bezel up and away from the

Bezel instrument.
Note
Do not pull bezel too far away from
instrument. Several cables must be
removed before completely removing the
bezel from the instrument.
2. Disconnect the following:

o Ground wire to chassis [7]
o Power [3] and data [2] (ribbon)
cable connectors from the rear of
the floppy disk drive [4]
o Ribbon cable connector J2 [5] on
the Touch Pad Interconnect
PCB [6].
Note
Mark all cable connectors and note pin 1
(red stripe on ribbon) on the floppy drive
data cable.
3. Remove the screw [8] (Phillips-head)

securing the LCD Interconnect PCB
access cover [1].
4. Disconnect the ribbon cable connected to
JP1A [9] on the LCD Interconnect
PCB [10].
5. Remove the bezel.
Replacement
Install 1. Install in reverse order of disassembly.

Bezel
Note
During assembly, verify that all cables/connections are secured and are not pinched
between the cover and the chassis.
Verification
Verification of Membrane 1. Power ON instrument, and allow it to initialize.

Keypad Operation 2. During initialization, verify that the LED on the floppy disk drive turns on
and that the LCD display screen is operational.
3. Once instrument is initialized, press RUN to prime system.
4. When in READY mode, press keys on membrane to check for proper
instrument response.
5. If system does not respond, check membrane keypad connection.
Removal
Remove 1. Remove all cable connectors

Right Cover (keyboard [3], COM1 [4], COM2 [5],
LPT1 [6]) from the right side of the
instrument.
2. Using a Phillips-head screwdriver, remove
the left and right screws [2] securing the
right side cover to the instrument frame.
3. Slide the right side cover [1] to the rear of
the instrument about 1/2-inch to clear the
bezel locking nuts, and lift off.
Replacement
Install Right Cover 1. Install in reverse order of disassembly.
Verification
Verification of Proper Cover 1. Verify that cutouts on front and rear portion of cover are placed
Installation under locking nuts.
2. Be sure cover is secure against right side of instrument.
Removal

Left Cover the left and right screws [2] securing the
left side cover [1] to the instrument frame.
2. Slide the left cover to the rear of the
instrument about 1/2-inch to clear the
bezel locking nuts and lift off.
Replacement
Install Left Cover 1. Install in reverse order of disassembly.
Verification

Verification of Proper Cover 1. Verify that cutouts on front portion of cover are placed under
Installation locking nuts.
2. Be sure cover is secure against left side of instrument.
Flow Panel (CD18-B1)
Removal
Prerequisite 1. Verify that the instrument is ON.

Front Cover
Remove Lower 1. Remove Lower Front Cover

Front Cover (RR-A1.02 Lower Front Cover).
Remove RBC 1. From the MAIN MENU, press

Aperture Plate [SPECIAL PROTOCOLS] then
[MORE] followed by [DRAIN
BATHS]:
2. Locate the von Behrens transducer
and swivel the red Aperture Plate
Retaining Arm [2] outward.
3. Pull the Aperture Plate [1] straight
out.
Remove WBC 1. Repeat the same procedure to

Aperture Plate remove the WBC Aperture Plate.
Note
Note the location of the cutout notch
and WBC etched in the Aperture
Plate.
Replacement

Aperture
Plate Note
The cutout notch [3] in the Aperture Plate
must be facing down and toward the
instrument when the plate is reinserted into
the transducer. Also note the RP etched in
the RBC Aperture Plate and WBC in the
WBC Aperture Plate.
Verification
Verify Background 1. Run a normal background count cycle and verify results are within acceptable
Counts limits. Refer to Specifications & Checklist.
Verify Quality 1. Run three (3) levels of CELL-DYN controls and verify results are within
Controls acceptable assay limits.
Both the von Behrens RBC and WBC transducers are removed according to the following procedure:
Removal
Prerequisite 1. If any fluid remains in the

transducers, make sure the
instrument is turned ON.
2. From the MAIN MENU, press
[SPECIAL PROTOCOLS] then
[MORE] followed by [DRAIN
BATHS].
3. Turn the instrument OFF after
the transducers are drained.
Open Upper Front 1. Open the upper front cover.

Cover
Remove Lower Front 1. Remove Lower Front Cover

Cover (RR-A1.02 Lower Front Cover).
Remove Top Cover 1. Remove Top Cover (RR-A1.03

Top Cover).
Remove Bezel 1. Remove the bezel (RR-A1.04

Bezel).
Remove PAM PCB 1. Remove the Pre-Amplifier

Module (PAM) PCB (RR-F1.01
PAM (Pre-Amplifier Module)).
Prepare to Remove 1. Route cable connector J3

von Behrens WBC (connected to PAM) through the
Transducer Flow Panel.
2. Label and disconnect all
tubing [1] from the von Behrens
WBC Transducer [3].
Remove von Behrens 1. Using a Phillips-head
WBC Transducer screwdriver, remove the three
(3) mounting screws [2] from the
transducer holding plate.
2. Cut all cable ties securing the
transducer cable to other
cables/chassis.
3. Route transducer cable away
from front panel and remove
transducer.
Prepare to Remove 1. Route cable connector J4

von Behrens (connecting to PAM) through the
RBC/PLT Transducer Flow Panel.
2. Label and disconnect all
tubing [3] from the von Behrens
RBC/PLT Transducer [1].
Remove von Behrens 1. Using a Phillips-head

RBC/PLT Transducer screwdriver, remove the three
(3) mounting screws [2] from the
transducer holding plate.
2. Lift the transducer assembly [1]
out of the instrument.
Replacement
Install von Behrens Transducer 1. Install in reverse order of disassembly.
Verification
Verify Gain Settings 1. Perform VP-09 Signal Processor Module (SPM) Verification/Adjustment.
WBC Metering PCB Removal
Removal
Prerequisite 1. Instrument must be in Initialized or

Ready mode.
2. From the MAIN menu, press [SPECIAL
PROTOCOLS] then [MORE] followed
by [DRAIN BATHS]:
3. When the drain baths procedure is
complete, turn OFF the instrument.

Front Cover
Remove Lower 1. Remove Lower Front Cover (RR-A1.02

Front Cover Lower Front Cover).
Remove Bezel 1. Remove the bezel (RR-A1.04 Bezel).
Remove 1. Using a Phillips-head screwdriver,

Plastic Shield remove the two (2) screws on the
metering PCB transparent plastic
shield.
Disconnect 1. Disconnect the tubing [4] at the top and

Metering Tube bottom of the metering tube [3].
Tubing
Caution
When removing tubing, take extreme
care not to break the glass ends. The
metering tube ends are fragile.
2. Use absorbent tissue to remove any

fluid in the metering tube.
Caution
Take extreme care not to allow the
liquid to touch the printed circuit
boards; it can short them out when
power is turned on. If the board does
get wet, make sure it is dry before
turning power back ON.
Remove WBC 1. Using a 1/4 inch nut driver, remove the

Metering PCB two (2) standoffs [7] on the board.
2. Remove the ground wire [5] on the
bottom standoff.
3. Disconnect cable connector J1 [6] on
the metering PCB.
4. Using a Phillips-head screwdriver,
remove the two (2) mounting screws [2]
securing the metering PCB to the Flow
Panel.
5. Lift the metering PCB [1] off of the Flow
Panel.
RBC/PLT Metering PCB Removal
Removal
Prerequisite 1. Instrument must be in Initialized or

Ready mode.
2. From the MAIN menu, press [SPECIAL
PROTOCOLS] then [MORE] followed
by [DRAIN BATHS]:
3. When the drain baths procedure is
complete, place the instrument in
Standby and turn OFF the power.

Front Cover


Plastic Shield remove the two (2) screws on the
metering PCB transparent plastic
shield.
Disconnect 1. Disconnect the tubing [4] at the top and

Metering Tube bottom of the metering tube [3].
Tubing
Caution
When removing tubing, take extreme
care not to break the glass ends. The
metering tube ends are fragile.
2. Use absorbent tissue to remove any

fluid in the metering tube.
Caution
Take extreme care not to allow the
liquid to touch the printed circuit
boards; it can short them out when
power is turned on. If the board does
get wet, make sure it is dry before
turning power back ON.
Remove 1. Using a 1/4 inch nut driver, remove the

RBC/PLT two (2) standoffs [7] on the board.
Metering PCB 2. Remove the ground wire [5] on the
bottom standoff.
3. Disconnect the cable connector J1 [2]
on the side of the metering PCB.
Note
The RBC Metering PCB [1] is installed
upside down. Push the metering tube
all the way down to access the top
screw securing the metering board to
the flow panel.

remove the two (2) mounting screws [6]
securing the metering PCB to the Flow
Panel.
5. Lift the metering PCB off of the flow
panel.
Replacement
Install Metering PCB 1. Install in reverse order of disassembly.
Note
Verify that tubing is not pinched when installing the plastic shield.
Verification
Verify Count 1. Verify appropriate count times (see VP-04 Metering System Timing Adjustments -
Times RBC and WBC:
o Verify RBC/PLT count times after installing RBC/PLT metering PCB.
o Verify WBC count times after installing WBC metering PCB.
Removal
Prerequisite 1. From the MAIN MENU, press

[SPECIAL PROTOCOLS] then
[MORE] followed by [DRAIN BATHS]:
2. Turn instrument power OFF.

Front Cover

Remove Top 1. Remove Top Cover (RR-A1.03 Top

Cover Cover).
Open Top Inner 1. Using a Phillips-head screwdriver,

Cover loosen the two (2) screws [1] securing
the top inner cover [2].
2. Lift the top inner cover upward and to
the right.
Note
This cover is hinged and stands in a
folded back position without support.
Remove Top 1. Using a Phillips-head screwdriver,

Shield from loosen the two (2) screws [4] securing
PAM PCB the top shield [1] on the PAM PCB [2].
2. Remove the shield.
Route HGB 1. Disconnect the cable connector from

Cable J2 [3] and route the HGB [7] cable
through the hole [5] at the right side of
the WBC metering PCB [6] and pull it
through the hole.
Remove Tubing Caution

The tubing is stiff. Use care when
removing the tubing to avoid breaking
the HGB Flow Cell.
1. Remove the black tubing from the

T-fitting [2] above and below the HGB
Flow Cell [1].
Note
Use absorbent tissue to capture any
excess liquid in the HGB Flow Cell.

Hemoglobin remove the two (2) screws [3]
Flow Cell securing the HGB flow cell to the flow
panel.
2. Route the HGB cable from behind the
tubing installed at solenoid 2-6 and
remove the HGB Flow Cell module.
Replacement
Install Hemoglobin Flow Cell 1. Install in reverse order of disassembly.
Verification
Verify HGB Offset, Gain, 1. Perform HGB Self Test and Adjust HGB Gain (see Pre-Amp Alignments).
Precision 2. Verify HGB precision.
Verify Background 1. Run a normal background count cycle and verify results are within
Counts acceptable limits. Refer to Specifications & Checklist.
Verify Quality Controls 1. Run three (3) levels of CELL-DYN controls and verify results are within
acceptable assay limits.
Fluid Power Supply (CD18-C1)
Removal
Prerequisite 1. Place the instrument in Standby and

turn OFF the power.

Cover
Cover).
Open Top 1. Open Top Inner Cover (RR-B1.04

Inner Cover Hemoglobin Flow Cell).
Remove Note
Tubing Cut any cable ties on cables
surrounding the fluid power supply
assembly as needed.
1. Label and disconnect the tubing from

the T-connector at the top of
solenoid 5-6 [4].
solenoid 5-5 [3].
3. Label and disconnect the silicone
tubing [1] from the top of the 0.5 psi
accumulator located at the rear of the
fluid power supply.
solenoid 5-1 [2].
5. Disconnect the tubing [5] from the
chassis floor fitting [6] next to the left
pump assembly.
6. Disconnect the tubing [7] connected to
the reagent panel waste line (at the rear
of the Reagent Inlet Panel).
Remove 1. Locate solenoid 1-6 and its

Tubing corresponding silicone tubing [1].
(continued) Follow the tubing to the back of the
Flow Panel, then label and disconnect
the tubing at the in-line connector.
2. Remove the clamp [2] and plug [3] from
the Vacuum Accumulator drain line [4],
and pull the tubing through the rear of
the Reagent Inlet Panel [5].
Disconnect 1. Disconnect cable connector J6 [3] from
Connectors the Pump Relay PCB [1].
2. Disconnect cable connector J1 [2] from
the Pump Relay PCB [1].
3. Disconnect cable connector J1
(three-pin cable) from the internal waste
sensor PCB located directly under the
Pump Relay PCB.
4. Disconnect cable connector J9 [3] from
the Solenoid Driver board.
5. Disconnect cable connector J10 [4]
from the Solenoid Driver board.
(bottom connector) from the Solenoid
Driver Module (SDM).
Remove Fluid 1. Using a Phillips-head screwdriver,
Power Supply remove the three (3) corner mounting
screws and the one (1) central
mounting screw securing the Fluid
Power Supply to the base of the
chassis.
2. Lift the Fluid Power Supply directly up
and out of the instrument.
Note
Verify that all cable connectors and
tubing are disconnected.
Replacement
Install Fluid Power Supply 1. Install in reverse order of disassembly.
Verification
Verify Vacuum, Pressure, 1. Perform VP-03 Vacuum and Pressure Adjustments.

Count Times 2. Verify all count times (see VP-04 Metering System Timing
Adjustments - RBC and WBC).
Syringe Assembly (CD18-E1)
Removal

turn OFF the power.

Front Cover


Cover Cover).

Remove 1. Mark and remove the cable connectors

Connectors at the top of the cable distribution
board [1]. There are four cable
connectors that must be unplugged
from this board: J20, J21, J22, and
J23.
2. Cut all cable ties securing cables to
other cables (there are approximately
6-8 cable ties).
Remove Wash 1. Using a 3/32-inch hex wrench, remove
Block Tubing the clamp securing the aspiration
tubing [3].
2. Using an adjustable wrench, loosen the
screw securing the two (2) clamps [2]
securing the wash block tubing.
3. Label and disconnect the two (2) tubes
from the Wash Block (top is diluent and
bottom is waste).
4. Route the clamps with attached tubing
through the cutout [4] on the left side of
the Sample Probe Driver assembly
chassis [2].
Remove 1. Disconnect the tubing from the top of

Sample Probe the Sample Probe.
Tubing
Remove 1. Disconnect the cable connectors

Sample Probe J1 [2] [5] (ribbon) and J2 [3] [6] (power)
Motor Cable from both the top [1] and bottom [4]
chopper PCBs.
Sample Probe remove the two (2) top and two (2)
Assembly bottom mounting screws [1] securing
the Sample Probe Driver Assembly to
the chassis.
2. Lift the Sample Probe Driver Assembly
out of the instrument.
Note
Before lifting the Sample Probe Driver
Assembly completely out of the
instrument, check to make sure that all
cables are disconnected. Disconnect
any cables that you find still attached,
making sure to mark them before
unplugging.
Replacement
Install Sample Probe Assembly 1. Install in reverse order of disassembly.
Verification
Verify Normal Operation 1. Run three (3) cycles and verify normal operation, e.g. binding, liquid
leaks, etc.
Verify Sample Probe 1. Perform VP-12 Sample Probe Alignment Check.

Alignment
Hazard
Removal

turn OFF the power.

Cover Cover).
Open Top Inner 1. Open Top Inner Cover (RR-B1.04

Cover Hemoglobin Flow Cell).
Remove Left Side 1. Remove the left side cover (see

Cover RR-A1.06 Left Cover).
Remove Fan Note

The fan above the Diluent Syringe
Driver Assembly must be removed
before the driver assembly can be
removed.

remove the two (2) top fan
screws [1].
Note
Screws are secured by a nut. Verify
that ALL nuts are removed prior to
removing fan.

loosen the two (2) bottom fan
bracket screws [2].
Note
If necessary, rotate the split pin
installed on the directional valve
drive linkage of the Diluent Syringe
to allow the fan to be removed.
3. Move the fan to the rear.
Disconnect 1. Disconnect the tubing [3] on both

Diluent Syringe sides of the directional valve [2] at
Driver Assembly the top of the Diluent Syringe [1].
Note
Use absorbent tissue to capture any
excess liquid.
2. Disconnect ribbon cable 9520444

from the back of the bottom chopper
PCB.
from the top chopper PCB.
Remove Diluent Note

Syringe Driver Lyse Syringe Driver Assembly [1],
Assembly Diluent Syringe Driver Assembly [2],
and Sample Syringe Driver
Assembly [3].
1. Using a 7/64-inch hex wrench,

remove the two (2) hex screws [5] on
the front of the base plate [4] located
at the bottom of the diluent driver
assembly [2].
loosen the hex screw [6] from the
rear base plate [4] of the Diluent
Syringe Driver Assembly [2].
3. Slide the Diluent Syringe Driver
Assembly [2] forward, tilt it back, and
remove it through the right side of
the chassis.
Replacement
Install Diluent Syringe Driver Assembly 1. Install in reverse order of disassembly.
Verification
Verify Operation 1. Verify VP-10 Diluent and Sample Verification/Adjustment.
RR-E1.04 Sample Syringe Driver Assembly
Removal
Prerequisite 1. Place the instrument in Standby

and turn OFF the power.

Cover Cover).


Remove Fan 1. Remove the fan located above the

Diluent Syringe Driver Assembly
(see Remove Fan, RR-E1.02
Diluent Syringe Driver Assembly).
Disconnect 1. Disconnect the tubing [2] on top of

Tubing the Sample Syringe Driver
Assembly [1].
Disconnect 1. Cut the cable ties securing the cable
Connectors harness to the back of the syringe
assembly.
from the chopper driver PCB.
Remove Sample Note

Assembly Diluent Syringe Driver Assembly [2],
and Sample Syringe Driver
Assembly [3].

remove the two (2) hex screws [5]
on the front base plate [4].
rear base plate [4] of the Sample
3. Slide the Sample Syringe Driver
Assembly [3] forward, tilt it back,
and remove it through the right side
of the chassis.
Replacement
Install Sample Syringe Driver Assembly 1. Install in reverse order of disassembly.
Verification
Verify Sample 1. Verify sample volume (VP-10 Diluent and Sample Verification/Adjustment).
Volume
Verify Quality 1. Run three (3) levels of CELL-DYN controls and verify results are within acceptable
Controls assay limits.
RR-E1.06 Lyse Syringe Driver Assembly
Removal

Remove Top Cover 1. Remove Top Cover (RR-A1.03

Top Cover).


Disconnect 1. Disconnect the tubing [2] from the

Tubing/Cables top of the Lyse Syringe [1].
from the chopper driver PCB.
Remove Lyse Note

Assembly Diluent Syringe Driver
Assembly [2], and Sample Syringe
Driver Assembly [3].

remove the two (2) hex screws [5]
on the front base plate [4].
rear base plate [4] of the Lyse
3. Slide the Lyse Syringe Driver
Assembly forward, tilt it back, and
remove it through the right side of
the chassis.
Replacement
Install Lyse Syringe Driver Assembly 1. Install in reverse order of disassembly.
Verification
Electronics / Card Cage (CD18-F1)

RR-F1.01 PAM (Pre-Amplifier Module)
Removal

turn OFF the power.
Front Cover


Cover Cover).
Flip Bezel Latch 1. Using a Phillips-head screwdriver,

Bracket UP remove the lower screw [1] on the
bezel latch bracket [2] attached to the
chassis.
2. Loosen, but do not remove the top
screw [3] on the latch.
3. Flip the latch up and to the right.
Remove Top 1. Using a Phillips-head screwdriver,

Shield from loosen the two (2) screws [4] securing
PAM PCB the top shield [5] on the PAM PCB.
2. Remove the shield.
Remove PAM 1. Using a Phillips-head screwdriver,

Cover remove the two (2) left retaining
screws [4] [6] on the left side of the
PAM cover plate and loosen the two
(2) right retaining screws [7].
2. Slide the PAM cover [8] to the left and
off the PAM PCB.
Disconnect 1. Mark and disconnect the five (5) cable

Cables connectors J1 [1], J2 [2], J3 [3],
J4 [4], and J5 [5] from the PAM PCB.
Note
The J3 connector is for WBC and the
J4 connector is for RBC/PLT.
Remove PAM 1. Using a 1/4-inch nut driver, remove

Board the four (4) metal standoffs [6], on the
corners of the PAM PCB.
2. Lift the PAM PCB off the Flow Panel.
Replacement

PAM
Board Note
Be sure that the metal standoffs securing the PCB in place and the screws securing the
metal cover over the PCB are securely fastened. Failure to properly secure the standoffs
and cover screws may result in electrical noise being induced due to improper grounding.
Verification
Verify PAM Printed Circuit Board 1. Perform VP-08 Pre-Amplifier Module (PAM) Adjustment.
Adjustments
Verify SPM Printed Circuit Board 1. Perform VP-09 Signal Processor Module (SPM)
Adjustments Verification/Adjustment.
RR-F1.02 MPM (Motor Processor Module) Board

Removal

turn OFF the power.

Cover Cover).

Remove 1. Label and disconnect cable connectors

Cables (leading to the MPM PCB):
o J1 [1]
o J2 [2]
o J3 [3]
o J4 [4]
o J5 [5]
o J6 [6]
o J7 [7]
o J10 [8]
Remove MPM 1. At each corner of the MPM PCB,

Board compress the plastic snap-in pins and
pull the corner of the PCB free from the
pin.
Note
Repeat this procedure for the snap-in
pin at the center of the PCB.
2. Lift the MPM PCB off the top inner

cover.
Replacement
Install MPM Board 1. Install in reverse order of disassembly.
Verification
Verify Motor Operation 1. Perform VP-11 Stepper Motor Power Test and Verification.
RR-F1.03 CDM (Cable Distribution Module) Board
Removal

turn OFF the power.

Cover Cover).

Disconnect 1. Label and disconnect the following

Cables cable connectors (leading to the CDM
PCB):
o J1 [15]
o J2 [14]
o J3 [13]
o J4 [12]
o J5 [11]
o J6 [10]
o J7 [9]
o J8 [8]
o J9 [7]
o J11 [16]
o J14 [17]
o J15 [18]
o J16 [19]
o J17 [20]
o J18 [21]
o J19 [22]
o J20 [1]
o J21 [2]
o J22 [3]
o J23 [4]
o J31 [5]
o J32 [23]
Remove CDM 1. At each corner of the CDM PCB,

Board compress the plastic snap-in pin and
pull the corner of the PCB free from the
pin.
Note
Repeat this procedure for the snap-in
pin at the center of the PCB.
2. Lift the CDM PCB off the top inner

cover.
Replacement
Install CDM Board 1. Install in reverse order of disassembly.
Verification

Verify CDM PCB 1. Perform VP-07 Cable Distribution Module Test Points.
Voltage
Verify Normal Operation 1. Run three (3) cycles and verify normal operation, e.g. binding, liquid leaks,
etc.
RR-F1.04 Hard Disk Drive
Removal


Cover


Bezel).
Remove Right 1. Remove the right side cover

Side Cover (RR-A1.05 Right Cover).
Remove Front 1. Using a Phillips-head screwdriver,

Card Cage Shield remove the two (2) screws [1]
securing the Front Card Cage
Shield [2] to the chassis.
2. Remove the shield from the
instrument.
Prepare to 1. Disconnect the Backplane PCB
Remove Card Power cable from the DC Regulator
Cage from PCB.
Chassis 2. Disconnect the Disk Drive Power
Cable from the DC Regulator PCB.
3. Remove the two (2) screws securing
the top section of the backplane
PCB shield and remove the shield.
4. Disconnect cable connectors J9 and
J10 on the Backplane PCB.
Remove Card 1. Using a Phillips-head screwdriver,

Cage remove the four (4) screws [1]
securing the card cage [2] to the
chassis.
Caution
Once the screws are removed, the
card cage drops down. Excessive
movement on the card cage can
damage connectors on the
Backplane PCB.
Remove Hard 1. Slide the bottom of the card cage

Disk Drive forward and tilt it backward.
Note
Verify that all four (4) screws
securing the hard disk drive to the
card cage are accessible.

remove the four (4) screws [1]
securing the hard disk drive [2] to
the card cage.
3. Note the alignment of the red
stripe [5] on the ribbon cable in
relationship to the hard disk drive
connector.
Note
The same alignment must be
maintained during replacement.
4. Disconnect the power [3] and data

cable [4] connectors (rear of hard
disk drive).
5. Remove the hard drive from the
instrument.
Replacement

Install Hard Disk Drive 1. Install in reverse order of disassembly.
Verification
Verify Hard Disk Drive Setup 1. Perform VP-14 Hard Disk Drive Setup and Verification.
RR-F1.05 Floppy Disk Drive
Removal

turn OFF the power.

Front Cover

Remove Floppy 1. Using a Phillips-head screwdriver,

Disk Drive loosen the four (4) screws [1] securing
the floppy drive mounting bracket [2]
to the bezel.
securing the floppy drive to the
mounting bracket [4].
3. Remove the floppy disk drive from the
instrument.
Replacement
Install Hard Disk Drive 1. Install in reverse order of disassembly.
Verification
Verify Floppy Disk Drive 1. Power ON instrument, and allow it to initialize.

Operation 2. During initialization, verify that the LED on the floppy disk drive turns on and
that the LCD display screen is operational.
RR-F1.06 Signal Processor Module (SPM)
Removal

turn OFF the power.

Front Cover


Card Cage remove the two (2) screws [1]
Shield securing the Front Card Cage
instrument.
Remove SPM Note

PCB Card cage PCBs from left to right:
SPM, CCM, DLA and SBC.

from the SPM PCB [1].
2. Open the top and bottom plastic
mounting tabs.
Note
Pulls the board slightly out of the card
cage.
3. Pull the board out of the instrument.
Replacement
Install SPM 1. Install in reverse order of disassembly.

PCB
Note
When reinserting the SPM PCB into its slot at the rear of the card cage, press it firmly
into place to ensure proper seating.
Verification
Verify SPM PCB 1. Perform VP-09 Signal Processor Module (SPM) Verification/Adjustment.
Adjustments
Note
Make SPM adjustments in the following order:
o RBC
o RER
o WBC
o PLT
Verify Quality Controls 1. Run three (3) levels of CELL-DYN controls and verify results are within
RR-F1.07 Cell Count Module (CCM)
Removal

turn OFF the power.

Front Cover


instrument.
Remove CCM Note

1. Disconnect cable connectors J1

through J3, and J5 from the CCM
PCB.
2. Open the top and bottom plastic
mounting tabs [1].
Note
Pulls the board slightly out of the card
cage.
3. Pull the board out of the instrument.
Replacement
Install CCM 1. Install in reverse order of disassembly.

PCB
Note
When reinserting the CCM PCB into its slot at the rear of the card cage, press it firmly
Verification
RR-F1.08 Data Link Adapter (DLA)
Removal

turn OFF the power.

Front Cover


instrument.
Remove DLA Note

1. Disconnect cable connectors J1 [2]

and J2 [3] from the DLA PCB [1].
remove the screw securing the DLA
PCB bracket to the top of the card
cage.
3. Grasp the top and bottom of the DLA
PCB and pull the card from the card
cage and away from the instrument.
Replacement
Install DCM 1. Install in reverse order of disassembly.

PCB
Note
When reinserting the DLA PCB into its slot at the rear of the card cage, press it firmly
Verification
RR-F1.09 Single Board Computer (SBC)
Removal

turn OFF the power.

Front Cover


instrument.
Remove SBC Note

1. Label and disconnect all cable

connectors from the SBC PCB [1].
remove the screw securing the SBC
PCB bracket to the top of the card
cage.
3. Grasp the top and bottom of the SBC
PCB and pull the card from the card
cage and away from the instrument.
Replacement
Install SBC 1. Install in reverse order of disassembly.

PCB
Note
When reinserting the SBC PCB into its slot at the rear of the card cage, press it firmly
Verification

RR-F1.10 Card Cage Backplane PCB
Removal

turn OFF the power.

Front Cover


instrument.
Remove SPM 1. Remove SPM PCB.

PCB
Remove CCM 1. Remove CCM PCB.

PCB
Remove DLA 1. Remove DLA PCB.
PCB
Remove SBC 1. Remove SBC PCB.

PCB
Remove 1. Using a Phillips-head screwdriver

Backplane PCB (stubby), remove the top three (3)
Shield screws [2] securing the Backplane
PCB Shield [1] to the card cage [3].
2. Using a Phillips-head screwdriver
(stubby), remove the bottom three (3)
screws [4] securing the Backplane
PCB Shield [2] to the card cage [3].
3. Disconnect cable connectors J1, J9,
and J10 from the Backplane PCB.
instrument.
Remove 1. Verify that all screws and cable

Backplane PCB connections are removed from the
Backplane PCB.
2. Remove the Backplane PCB from the
instrument.
Replacement

Install Backplane 1. Install in reverse order of disassembly.
PCB
Note
When reinserting the backplane PCB into its slot at the rear of the card cage, press
it firmly into place to ensure proper seating.
Verification
Verify Normal Operation 1. Place instrument in Ready mode.

2. Verify that instrument properly initializes and primes.
Verify Card Cage Backplane 1. Perform VP-06 Card Cage Backplane Test Points.
PCB Voltages
Verify Background Counts 1. Run a normal background count cycle and verify results are within
acceptable limits. Refer to Specifications & Checklist.
Verify Quality Controls 1. Run three (3) levels of CELL-DYN Controls and verify results are within
LCD/Keyboard (CD18-G1)
Removal

turn OFF the power.
2. Unplug power cord from rear of
instrument.

Front Cover

Front Cover
(RR-A1.02 Lower Front Cover).

Bezel).
Remove LCD 1. Place bezel cover face down on a

Display Screen flat surface.
Assembly 2. Disconnect cable connector CN2 [3]
from the backlight inverter PCB [4].
3. Release the backlight wires from the
cable clip.
securing the LCD Display Screen
mounting plate to the bezel cover.
securing the LCD Display Screen [6]
to the mounting plate.
6. Disconnect the LVDS cable
connector [7] at the rear of the LCD
Display Screen.
Note
When disconnecting the LVDS cable
connector, pull the connector, NOT
the wires.
Replacement
Install the LCD Display 1. Install in reverse order of disassembly.

Screen Assembly
Note
During replacement, verify that the LCD Display Screen assembly is
centered in the bezel cover, before securing the assembly to the cover.
Verification
Verify LCD 1. Turn the instrument Power ON and

Display Screen allow the instrument to initialize.
Installation 2. Verify that the LCD Display Screen
is turned on and centered in the
bezel.
3. Verify that the LVDS cable (between
the LCD display [3] and the LCD
Interconnect PCB [1]) is routed
correctly [1-3]
Note
Failure to properly route and secure
the LVDS cable may result in
damage to the wires and connector.
Power Supply (CD18-H1)
Removal

Note
Never touch a PCB when the power
is ON.
2. Unplug power cord from rear of

instrument.

Cover


Cover Cover).

Bezel).
Remove Right 1. Remove the right side cover

Side Cover (RR-A1.05 Right Cover).
Remove Cable 1. Label and disconnect the four (4)

Connectors from cable connectors at the rear of the
Power Switch power switch [1].
Note
The power switch contains two (2)
black and two (2) white cable
connectors.
Remove Cable 1. Label and disconnect cable

Connectors from connectors J3 through J9, and J11
DC Regulator PCB from the DC Regulator PCB [2].
Remove Cable Note

Connectors from Cut cable ties from cable bundles
AC Regulator PCB containing cable connector J7.
1. Label and disconnect the cable

connectors J5, J7 and J9 from the
AC Regulator PCB [1].
2. Label and disconnect the chain
cable connector J6 from the AC
Regulator PCB and J2 from the DC
Regulator PCB.
3. Release the chain cable connector
from the cable clamp attached to
the switching power supply.
Remove Power 1. Using a Phillips-head screwdriver,

Supply Assembly remove the four (4) screws [1] from
the rear of the instrument securing
the power supply assembly to the
instrument chassis.
2. Carefully lift the power supply up,
then partially out of the instrument.
3. Verify that no cable connectors are
connected to the power supply
assembly.
Caution
The power switch is vulnerable to
damage during this process.
4. Remove the power supply

assembly.
Replacement
Install Power Supply Assembly 1. Install in reverse order of disassembly.
Verification
Verify System Voltages 1. Perform VP-06 Card Cage Backplane Test Points.
Verification Procedures
VP-01 Preparation for Alignment and Verification

VP-01 Preparation for Alignment and Verification
Purpose Preparation for Alignments/Verifications. Module CELL-DYN 1800
Materials Required See Test Equipment and Supplies Required. Time 01:00 hr
Test Equipment and Supplies Required
ITEM DESCRIPTION QTY
1 Digital Volt Meter 1
3 5-inch Jumper Leads 3
4 Stopwatch 1
5 Vacuum Gauge 0-30 Inches 1
6 Pressure Gauge 0-5 lb. 1
7 Pressure Gauge 0-10 lbs. 1
8 Polystyrene Microspheres, 5.0 or 5.01 µm 1
9 Polystyrene Microspheres, 3.31 µm 1
10 Hemostats 3
11 500 mL Flask or Beaker 1
12 Silicone Tubing AR
13 Standard Tool Kit 1
14 Fresh Blood Samples With Reference Values AR
15 Assayed Controls for CELL-DYN 1800 AR
16 20k Ohm 1% Resistor 1
17 15k Ohm 1% Resistor 1
18 10 mL Graduated Cylinder 1
19 40 µL Micropipettes AR
20 100 µL Micropipettes AR
21 50 mL Counting Cups AR
22 25 mL Graduated Cylinder 1
23 12-inch Ruler With 1/16 Increments 1
24 Oscilloscope (Optional) 1
AR = As Required
Preparation for Alignment/Verification
Perform the following procedure to prepare the CELL-DYN 1800 System for alignment/verification:
Check Reagents 1. Verify all reagents are correct and are 1/3 full.
Remove Upper 1. Remove Upper Front Cover (RR-A1.01 Upper Front Cover).
Front Cover
Remove Lower 1. Remove Lower Front Cover (RR-A1.02 Lower Front Cover).
Front Cover
Remove Left 1. Remove Left Cover (RR-A1.06 Left Cover).

Cover
Remove Right 1. Remove Right Cover (RR-A1.05 Right Cover).

Cover
Remove Top 1. Remove Top Cover (RR-A1.03 Top Cover).

Cover
Clean Aperture 1. Remove and clean both RBC/PLT and WBC aperture plates following the
Plates procedure in Section 9: Service and Maintenance, Subsection:
Cleaning/Replacing Aperture Plates of the CELL-DYN 1800 Operator's Manual.
Clean HGB Flow 1. Clean the HGB Flow Cell following the procedure in Section 9, Subsection:
Cell Cleaning the HGB Flow Cell of the CELL-DYN 1800 Operator's Manual.
Re-initialize 1. Re-initialize the instrument by turning the system OFF then ON again.
Instrument
Check Tubing, 1. When Initialization is complete, press [PRIME/RUN] to prime the instrument.
Look For Leaks Observe the flow system for leaks, tubing placement, pinched tubing, etc.
Install Covers 1. Reinstall the covers on the instrument.
Run Background 1. Run a Background count. Verify all background values are within the following
Count specifications:
WBC 0.5 K/µL
RBC 0.05 M/µL
HGB 0.1 g/dL
PLT 10.0 K/µL
Record 1. In the MAIN MENU, press [CALIBRATION].

Calibration 2. Record all Calibration Factors for Open and Pre-Dilute modes.
Factors
Record Dilution 1. Type 94043 to display the DILUTION FACTORS screen. Record all Dilution
Factors Factors.
Enter Service 1. In the MAIN MENU, type 999 for Operator ID and press Enter on the PC keyboard
Personnel ID or membrane keypad. This is to identify all runs performed by service personnel.
Order of Alignment/Verification
The following procedures are presented to ensure proper alignment of the CELL-DYN 1800 System.
Sequence Procedure
1 VP-03 Vacuum and Pressure Adjustments
2 VP-04 Metering System Timing Adjustments - RBC and WBC
3 VP-06 Card Cage Backplane Test Points
4 VP-07 Cable Distribution Module Test Points
5 VP-08 Pre-Amplifier Module (PAM) Adjustment
6 VP-09 Signal Processor Module (SPM) Verification/Adjustment
7 VP-10 Diluent and Sample Verification/Adjustment
8 VP-11 Stepper Motor Power Test and Verification
9 VP-12 Sample Probe Alignment Check
When performing adjustments:
• At the completion of each Vacuum and Pressure Adjustment procedure, verify the WBC and RBC count times and
normal system operation
• Prior to starting any electronic alignment, verify the power supply voltages in VP-06 Card Cage Backplane Test
Points and VP-07 Cable Distribution Module Test Points.
• Whenever an alignment adjustment is performed on the Pre-Amplifier Module (PAM), verify all parameters on the
Signal Processor Module (SPM).
VP-02 Decontamination
Purpose To decontaminate all surfaces before servicing. Module CELL-DYN 1800
Materials Required Sodium hypochorite solution Time 00:15 min
Decontamination 1. The OSHA Bloodborne Pathogen Rule (29 CFR Part 1910.1030) requires the
Ratio decontamination of laboratory equipment prior to servicing or shipment:
o Decontaminate the instrument by performing the Auto-Clean cycle. This
cycle flushes all of the fluid pathways with reagents to purge any waste from
the fluid pathways. The Open Mode Sample Probe is automatically rinsed
after every cycle. The surfaces of the instrument should be wiped with a
nonabrasive detergent solution to remove any soiling, then wiped with a
tuberculocidal disinfectant, such as a 0.5% sodium hypochlorite solution.
2. To calculate the percent (%) sodium hypochlorite concentration desired, use the
following formula:
a. A = Percent (%) of sodium hypochlorite solution desired
b. B = Percent (%) of sodium hypochlorite stock solution (as purchased)
c. X = Parts of water to be mixed with one part of the sodium hypochlorite stock
solution:
d. Example: If you need a 0.5% solution of sodium hypochlorite for a cleaning

procedure, and the label on the bottle of bleach states that it is 5.25%
sodium hypochlorite, then:
e. Add 9.5 parts deionized water to 1 part bleach to obtain a 0.5% sodium
hypochlorite solution, or 9.5 mL of deionized water to 0.5 mL of bleach
(5.25% sodium hypochlorite) to obtain 10 mL of a 0.5% solution of sodium
hypochlorite.
3. If the instrument is to be shipped, it must be decontaminated prior to shipment. This
is accomplished by pressing the [CLEAN FOR SHIPPING] key in the SPECIAL
PROTOCOLS menu. Instructions for this procedure are given in the
CELL-DYN 1800 Operator's Manual, (Section 9 Preparing the Instrument for
Extended Periods of Non-Use or Shipping).
VP-03 Vacuum and Pressure Adjustments
Purpose Align and adjust pressure and vacuum. Module CELL-DYN 1800
Materials Required Digital Volt Meter (DVM) Time 00:30 min

0-5 psi Pressure Gauge
0-30 psi Pressure Gauge
0-30 HG Vacuum Gauge
Phillips Screwdriver
Thin Flat Blade Screwdriver
This procedure consist of four parts (perform in sequence):
Regulator Alignment
Pressure Adjustment (0.5 psi)
Pressure Verification (High)
Vacuum Adjustment (8 inch)
The CELL-DYN 1800 System utilizes one vacuum and two pressure levels to accomplish the following tasks: moving sample,
reagents, and waste, bubble mixing of sample, and back flushing RBC and WBC apertures. The vacuum and bubble mix
pressures are adjustable by a solid-state regulator. The backflush pressure is not critical and the pump is under direct
computer control.
The solid state regulator has two input ports: P1 for pressure and P2 for vacuum. It also has jumper terminals which
accommodate all desired vacuum and pressure ranges. The jumper positions are:
Jumpers Pressure (psi) Vacuum (inches Hg)
1 A-B 0.0 - 3.0 0.0 - 6.0
2 C-D 2.5 - 5.1 5.0 - 10.2
3 E-F 4.3 - 6.6 8.6 - 13.2
4 G-H 6.0 - 8.0 12.0 - 16.0
Regulator Alignment
Access Pump Note

Relay Board This offset adjustment must be performed with no pressure or vacuum applied to
the regulator.
1. Disconnect the appropriate pumps on the Pump Relay Board and bleed off
pressure from the Accumulators.
o J4 for low pressure
o J2 for vacuum on new-style pumps
2. Remove the pressure or vacuum line from the top of the regulator.
Measure Voltage 1. On the Pump Relay Board the voltage should be 5.0 ± 0.15 volts at J7 pin 7 for
vacuum, and J8 pin 5 for pressure.
Note
You must remove the board mounting screws and free the rest of the board to
make the remaining adjustments. Disconnect J6 or J7 while relocating the
appropriate printed circuit board to avoid shorting out components.
2. Note the current position of jumper E1 and set E1 to the C-D position.
3. Connect the DVM (Digitial Volt Meter) negative lead to TP3 (GND) on regulator
board. Connect the positive lead to TP2 (REF). The voltage should be
1.00V ± 0.14 volts.
o If voltage is not correct, re-check Step 1.
o If voltage is still not correct, replace the regulator.
4. Connect the DVM negative lead to TP1 and the positive lead to TP2, and read the
voltage. Adjust R18 for a voltage of 0.000 ± 0.005.
Note
If voltage is negative, turn R18 (offset) clockwise; if the voltage is positive, turn R18
counterclockwise.
Reposition E1 1. Move jumper E1 back to the proper operating position.
Reconnect 1. Reconnect the pressure or vacuum line to the top of the regulator and reconnect
Pressure / Vacuum the cable(s) on the pump relay module.
Line
Pressure Adjustment (0.5 psi)
Follow the steps below for pressure adjustment:
Remove Top 1. Remove Top Cover (RR-A1.03 Top Cover).

Cover
Open Top Inner 1. Open Top Inner Cover (RR-B1.04 Hemoglobin Flow Cell).
Cover
Connect 1. Locate the small silicone tubing connected to the in-line fitting at top of the 0.5 psi
Pressure Gauge Pressure Accumulator (located closest to the rear of the instrument).
2. Connect a 0-5 psi gauge in-line with the silicone tubing and fitting (the Pressure
Accumulator is located on the part of the Fluid Power Supply which is closest to the
rear of the instrument).
Adjust 1. Adjust R16 on the Regulator board for 0.55 psi ± 0.02.
Regulator
Note
Clockwise adjustment increases pressure and counterclockwise adjustment
decreases pressure.
2. Remove the gauge and reconnect the silicone tubing to in-line connector.
Pressure Verification (High)
Follow the steps below for pressure verification:
Locate In-line 1. Locate solenoid valve 1-6 and trace the tubing back through the flow panel to the
Connector in-line connector.
Connect Pressure 1. Connect a 0-30 psi gauge in-line.

Gauge 2. In the RUN screen, press [CLEAR ORIFICE] while observing the gauge.
Verify Correct 1. When the pump activates, verify a pressure of no less than 4 psi.
Pressure o If the pressure is less than 4 psi, check for leaks. Replace the Pressure
Pump if necessary. (Refer to RR-C1.01 Fluid Power Supply.)
Vacuum Adjustment (8 inch)
Follow the steps below for solid state regulator adjustment:

Locate Vacuum Note

Regulator This is a coarse adjustment only. The fine adjustment is predicated upon metering
system count times. Make this coarse adjustment only if there is reason to believe that
the vacuum is grossly misadjusted.
1. Locate the solid state vacuum regulator on the rear side of the Fluid Power Supply
(closest to the flow panel).
Adjust R16 1. Remove the TYGON tubing from the top of the Vacuum Accumulator (located on the
back of the Fluid Power Supply closest to the flow panel) and connect a 0-30" Hg
gauge in-line between the tubing and the Accumulator.
2. Adjust R16 (accessible only from the front of the Fluid Power Supply) for
8.25" Hg ± 0.25".
Note
Clockwise adjustment increases vacuum and counterclockwise adjustment decreases
vacuum.
Reconnect 1. Once R16 is adjusted, remove the vacuum gauge and reconnect the tubing line to the
Tubing Line regulator.
VP-04 Metering System Timing Adjustments - RBC and WBC
Purpose Adjust Metering System to regulate count times. Module CELL-DYN 1800
Materials Required Phillips Screwdriver Time 00:15 min
This procedure consist of two parts:
Links
RBC Metering System Timing Adjustment
WBC Metering System Timing Adjustment

The instrument uses the Volumetric Metering process to regulate the count cycle and to ensure that a precise volume of
sample is analyzed for the measurement. This table lists the results of a fault report displayed on the screen if a flow error or
clog occurs during a run cycle.
Metering Timing Fault Report
Detector Masked Flow Error Time Valid Meniscus Time Clog Time
(in seconds) (in seconds) (in seconds) (in seconds)
WBC Upper 0 - 0.5 0.5 - 1.0 1.0 - 3.0 3.0+
WBC Lower 0 - 3.5 3.5 - 4.0 4.0 - 6.5 6.5+ or greater than the moving average
RBC/PLT Upper 0 - 3.0 3.0 - 4.0 4.0 - 6.0 6.0+
RBC/PLT Lower 0 - 5.5 5.5 - 6.0 6.0 - 7.5 7.5+ or greater than the moving average
This figure illustrates the timing relationships for WBC and RBC measurements.
CELL-DYN 1800 Metering Timing Chart (in Seconds)

For each transducer there are two distinct counting periods, T1 and T2. RBC Metering Tube and WBC Metering Tube
illustrate the counting periods for the RBC and WBC metering tubes, respectively.
RBC Metering Tube
WBC Metering Tube

All count times are based on the vacuum level of the 8-inch vacuum reservoir and tubing length. These count times are
critical in that all clog and flow system alarms are generated via these count times. The vacuum adjustment (Vacuum
Adjustment (8 inch)) sets the coarse vacuum requirement, but further adjustments are necessary to ensure proper timing for
the flow system alerts.
RBC Metering System Timing Adjustment
Follow the steps below for adjusting the timing of the RBC metering system:
Verify 1. Ensure that the RBC aperture plate has been removed, cleaned, and reinstalled following
Count the procedure in Section 9: Service and Maintenance, Subsection: Cleaning/Replacing
Time Aperture Plates of the CELL-DYN 1800 Operator's Manual.
2. Run a background count and verify that the RBC displayed count time (T2) is 6.7 seconds
± 0.2 seconds.
o If the specification is met, go to Step 4.
o If the specification is not met, go to Step 3.
3. Readjust the vacuum to correct the count time if the time is out of specification (refer to
Adjust R16, Step 2 of the Vacuum Adjustment (8-inch) procedure previously discussed).
4. From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].
5. Verify the time displayed for RBC UPTIME is 4.8 to 5.2 seconds. (This time, displayed in
milliseconds, represents T1, which is the time when the RBC valve 1-2 opens until the
meniscus reaches the upper detector.)
o If the specification is met, the procedure is complete.
o If the specification is not met, go to Step 6.
6. Adjust the metering tube.
o Raise the metering tube to increase the upper time.
o Lower the metering tube to decrease the upper time.
7. Repeat Step 2 through Step 5 until the RBC Up Time/Count Time is within specification.
WBC Metering System Timing Adjustment
Follow the steps below for adjusting the timing of the WBC metering system:
Prerequisite Note
The RBC count times (T1 & T2) must be within specification before performing this
procedure.
Verify Count 1. Remove and clean the WBC aperture plate as described in Section 9: Service and
Time Maintenance, Subsection: Cleaning/Replacing Aperture Plates of the
CELL-DYN 1800 Operator's Manual.
2. Run a background count and verify the count time displayed to the right of the WBC
histogram is 5.0 ± 0.5 seconds.
Note
There is no count time adjustment procedure.
3. From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].

4. Verify that the time displayed for WBC UPTIME is 1.8 to 2.2 seconds. (This time
represents T1 which is the time when the WBC valve 4-3 opens until the meniscus
reaches the upper detector. See WBC Metering Tube.)
o If the WBC UPTIME specification is met, the procedure is complete,
o If the WBC UPTIME specification is not met, go to Step 5.
5. Adjust the metering tube.
o Raise the metering tube to increase the upper time.
o Lower the metering tube to decrease the upper time.
6. Repeat Step 1 through Step 4 until WBC UPTIME/COUNT TIME is within specification.
VP-05 CMOS Setup Verification/Adjustment
Purpose Provide instructions to verify and/or modify CMOS settings. Module CELL-DYN 1800
Materials Required None Time 00:10 min
Preparation 1. Power OFF the system.
Access CMOS Setup 1. Power ON the analyzer and press the Delete key, when prompted, to display
Utility Screen the CMOS Setup Utility Screen.
1. Use the up/down arrow keys to select the STANDARD CMOS SETUP.
2. Press the Enter key.
3. Refer to the figure (H2_6006) and make any necessary adjustments to the
Standard CMOS Setup screen.
Note
Verify/Modify
Press the up/down arrow keys to select a setting and then press PgUp/PgDn
Standard CMOS
keys to modify.
Setup
4. Press ESC to exit the screen.

1. Use the up/down arrow keys to select the ADVANCED CMOS SETUP.
3. Refer to the figure and make any necessary adjustments to the Advanced
CMOS Setup screen.
Note
keys to modify.
Verify/Modify
Advanced CMOS
Setup
1. Use the up/down arrow keys to select the ADVANCED CHIPSET SETUP.
3. Refer to the figure and make any necessary adjustments to the Advanced
Chipset Setup screen.
Note
keys to modify.
Verify/Modify
Advanced Chipset
Setup
Note
The SAMSUNG LCD assembly is Type 8, while the TOSHIBA LCD assembly is Type 7.
1. Use the up/down arrow keys to select the POWER MANAGEMENT SETUP.
3. Refer to the figure and make any necessary adjustments to the Power
Management Setup screen.
Note
Verify/Modify Power Press the up/down arrow keys to select a setting and then press PgUp/PgDn
Management Setup keys to modify.

1. Use the up/down arrow keys to select the PERIPHERAL SETUP.
3. Refer to the figure and make any necessary adjustments to the Peripheral
Setup screen.
Note
keys to modify.
Verify/Modify
Peripheral Setup
Save CMOS Settings 1. Press F10.
and Exit CMOS Setup
The message Save current settings and exit (Y/N)? displays.
2. Type Y and press Enter.
Note
Saves the CMOS settings, exits the CMOS Setup Utility and restarts the
computer.
Verification 1. Verify that the system boots up properly to the application software.
VP-06 Card Cage Backplane Test Points
Purpose Identify test points on Backplane PCB. Module CELL-DYN 1800
The following table lists the test points located on the backplane board and the figure illustrates the test points on the
backplane board.
Test Function Range Ripple
TP1 +5V +5.00V ± 0.20V
TP2 +12V Analog +12V ± 0.50V
TP3 -12V Analog -12V ± 0.50V
TP4 +12V Digital +12V ± 0.50V
TP5 -12V Digital -12V ± 0.50V
Card Cage Backplane (Rear Side)

Card Cage Backplane (Card Side)
1 LD4 +12VDC (DIG) 4 LD2 -12VDC (ANALOG)
2 LD5 -12VDC (DIG) 5 LD1 +5VDC
3 LD3 +12VDC (ANALOG)
Note
LEDs indicate voltages supplied to the board.
VP-07 Cable Distribution Module Test Points

Purpose Identify test points on CDM. Module CELL-DYN 1800
The following table lists the test points located on the Cable Distribution Module (CDM).
Location
Pin Function Voltage Ripple
(J5 and J8)
1 Brown Ground < 0.05V N/A
2 Blank N/A N/A N/A
3 Orange Ground < 0.05V N/A
4 Yellow +14V ±0.5V N/A
5 Green +28V ±1.00V N/A
6 Blank N/A N/A N/A
Note
On the CDM, +28V is used to initially energize solenoids and +14V is used to keep them closed.
VP-08 Pre-Amplifier Module (PAM) Adjustment
Purpose Adjust HGB self test and gain. Module CELL-DYN 1800
Adjust RBC/PLT and WBC aperture currents.

Flat Blade Screwdriver
5-inch Jumper Leads
20K Ohm 1% Resistor
15K Ohm 1% Resistor
The Pre-Amplifier Module (PAM) is located on the upper right corner of the Flow Panel. Hemoglobin circuitry, and the
RBC/PLT and WBC aperture currents require verification and/or adjustment on this module. The HGB flow cell should be
cleaned before performing hemoglobin alignments (refer to Section 9, Subsection: Cleaning the HGB Flow Cell in the
CELL-DYN 1800 Operator's Manual).
Pre-Amplifier Module (PAM) Test Points
The following table lists the test points located on the PAM.
Location Function Voltage
TP5 Analog Ground N/A
TP4 +100V +100V ± 0.5V
TP7 HGB Self Test/Gain Adjust +1.84V ± 0.02V (Self Test)

+5.2V ± 0.2V (Gain Adjust)
Test points for the PAM are shown in the figure below.
Pre-Amp Module (PAM) Test Points
Follow the steps below to align the PAM:

Open Upper Front Cover 1. Open the upper front cover.
Remove Lower Front Cover 1. Remove Lower Front Cover (RR-A1.02 Lower Front Cover).
Remove Top Cover 1. Remove Top Cover (RR-A1.03 Top Cover).
Remove Pre-amp Cover 1. Remove the pre-amp cover (RR-F1.01 PAM (Pre-Amplifier Module)).
HGB Self Test 1. Remove jumper JMP3 (lower right corner).

2. Press the touch plate to cycle the instrument and fill the HGB flow cell
with fresh reagent.
3. Connect the positive lead of the DVM to TP7.
4. Connect the ground of the DVM to TP5.
5. Adjust R14 to 1.84 volts ± 0.02 volts.
6. Verify the voltage at TP7.
7. Connect jumper JMP3 and verify voltage at TP7 returns to 5 volts.
Note
If voltage remains unchanged, the jumper may be in the wrong position.
Adjust HGB Gain 1. Press the touch plate to cycle the instrument and fill the HGB flow cell
with fresh reagent.
3. Adjust R4 (HGB Gain Adjust) for 5.2 volts ± 0.2 volts.
4. Press the touch plate to cycle the instrument and fill the HGB flow cell
with fresh reagent.
Note
If voltage does not meet specification, repeat Step 2 and Step 3.
Verify +100 Volts 1. Connect the positive lead of the DVM to TP4, and the negative lead to
TP5 (Gnd).
2. Verify 100 volts ±0.5 volts.
Adjust RBC/PLT Aperture 1. Disconnect the RBC signal cable from J4.
Current 2. Connect a 20K Ohm 1/4 watt resistor across pins 4 and 5 of J4.
3. Connect the DVM across the resistor.
4. Run a background count.
5. Adjust R72 (RBC/PLT aperture current adjust) for 11.0 volts ± 0.05 volts.
6. Remove the resistor and reconnect cable to J4.
Adjust WBC Aperture 1. Disconnect the WBC signal cable from J3.
Current 2. Connect a 15K Ohm 1/4 watt resistor across pins 4 and 5 of J3.
3. Connect the DVM across resistor.
4. Run a background count.
5. Adjust R66 (WBC aperture current adjust) for 12.0 volts ± 0.05 volts.
6. Remove the resistor and reconnect cable to J3.
Install Covers 1. Reinstall the top cover, upper and lower front covers, bezel and pre-amp
cover.
The following table contains the specifications for the Pre-Amp Module alignment.
Pre-Amp Alignments
HGB SELF TEST TP7 R14 +1.84V ± 0.02 V
HGB GAIN TP7 R4 5.2 V. ± 0.2 V
RBC/PLT APERTURE CURRENT 20K Ohm Resistor R72 11.0 V. ± 0.05 V
WBC APERTURE CURRENT 15K Ohm Resistor R66 12.0 V. ± 0.05 V
VP-09 Signal Processor Module (SPM) Verification/Adjustment
Purpose Adjust WBC, RBC, PLT Gain. and RBC Edit Ratio (RER) percentage (%). Module CELL-DYN 1800

Thin Flat Blade Screwdriver
Polystyrene Microspheres, 5.0 or 5.01 µm
Polystyrene Microspheres, 3.31 µm
Clean 50 mL Container
Note
The Signal Processor Module (SPM) is located in the main electronics card cage. Alignment of the WBC, RBC, and
PLT gains are critical adjustments that must be verified and/or adjusted before instrument accuracy can be
established. It is important to maintain the adjustment order for this verification procedure. The adjustment order is:
Sequence Activity
1 RBC Gain
2 RER Adjustment
3 WBC Gain
4 PLT Gain
Latex particles are used to perform these adjustments. The particles must be mixed vigorously before diluting to obtain
accurate results.
The Gain and RBC Cell Editing adjustments are performed in the Gain Adjust Mode, which allows multiple counts to be run
on the same sample. When Gain Adjust Mode is entered, whatever is in the Pre-Mix Cup is transferred to the WBC Cup and
5 mL of diluent is left undisturbed in the RBC Cup.
When performing RBC or PLT adjustments, 10 mL of diluent must be placed in Pre-Mix Cup before entering Gain Adjust
Mode to prevent air from being pulled into the von Behrens WBC Transducer during a count cycle.
Test points for the SPM are shown in the figure below:
SPM Test Points

RBC Gain
Prepare Latex 1. Pour 10 mL of diluent into the Pre-Mix Cup and add 1 drop of well-mixed 5.0 latex
Dilution particle solution to the 5 mL of diluent already in the RBC Mixing Chamber.
2. From the RUN screen, press [SPECIMEN TYPE].
3. Press Shift and # (simultaneously) to activate the Gain Adjust Mode.
Note
The message <PREPARING FOR GAIN ADJUST.> displays and WBC dilution is
transferred to the WBC Mixing Chamber. When the preparation cycle is complete,
the RUN screen displays.
Set RBC Gain 1. Press the Touch Plate to run a cycle.
Note
Always return to the RUN screen to run a cycle.
Note
When the procedure is completed, or if there is a need to exit the Gain Adjust Mode
while performing this procedure, such as constant clogs or improper dilution ratio,
press [SPECIMEN TYPE] followed by [PATIENT SPECIMEN].
Note
The RBC count should be between 3.5 and 4.5. If the count is outside this range,
modify the dilution ratio of the latex particles, exit Gain Adjust Mode and try again.
2. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].

3. Press [SMOOTHING OFF].
Note
The key changes to [SMOOTHING ON] and is highlighted, indicating the smoothing
function is ON.
4. Press [RBC HISTOGRAM].

5. Observe the lower part of the histogram data to determine if the channel number for
peak of 100 is Channel 98 ± 1 channel.
o If the specification is met, go to RER Adjustment.
o If the specification is not met, adjust R27.
Note
Turning R27 clockwise decreases the channel number; turning R27
counterclockwise increases the channel number.
6. Return to the RUN screen and run another cycle.
7. Observe the channel number for peak count of 100.
o If the gain is still outside of specification, repeat this process until the peak
count meets the specification (100 is in Channel 98 ± 1 channel). Refer to
RBC Gain Adjustments (Example)
Note
Turning R27 clockwise decreases the channel number; turning R27
counterclockwise increases the channel number.
Continue with 1. Go to RER Adjustment.

RER Adjustment
RBC Gain Adjustments (Example)
RER Adjustment
Determine 1. Verify that the dilution level on both the WBC

Adequate and RBC transducers (left side) is above the
Dilution Amount aperture (orifice) plates.
o If the dilution level in transducers is
met, go to Determine Edit Ratio
Percentage.
o If the specification is not met, go to
Step 2.
2. From the RUN screen, press [SPECIMEN
TYPE], then [PATIENT SPECIMEN].
3. Press the touch plate to run a cycle (exits Gain
Adjust Mode).
4. Pour 10 mL of diluent into the Pre-Mix Cup and
add 1 drop of well-mixed 5.0 µm latex particle
solution to the 5 mL of diluent already in the
RBC Mixing Chamber.
5. In the RUN screen, press [SPECIMEN TYPE].
6. Press Shift and # (simultaneously) to activate
the Gain Adjust Mode.
Note
The message <PREPARING FOR GAIN ADJ.>
displays and diluent in the Pre-Mix Cup is
transferred to the WBC Mixing Chamber. When
the preparation cycle is complete, the RUN
screen displays.
Determine Edit 1. Press the Touch Plate to run a cycle. Make a

Ratio note of the RBC results.
Percentage
Note
The RBC count should be between 3.5 and 4.5.
If the count is outside this range, adjust the
dilution ratio of the latex particles by adding
more diluent or latex to the RBC Mixing
Chamber.
2. Repeat Step 1 two more times for a total of

three (3) runs. Calculate the average of the
three RBC counts.
3. Refer to Cell Edit Table, to find the target value
for the following:
a. Use the average RBC calculated in
Step 2 to determine the Edit Ratio
Percentage that coincides with the
calculated average.
Note
Example: A count of 4 million relates to an edit
ratio of 28.0% ± 1.3.
Adjust RER Note Cell Edit Table

Use the edit ratio calculated in Determine Edit
Ratio Percentage above to perform the
following procedure:
1. From the MAIN Menu, press [DIAGNOSTICS]

followed by [RAW DATA].
2. Observe the RBC RER displayed on the screen
from the last cycle.
o If the specification is met (see Cell Edit
Table), go to WBC Gain.
o If the specification is not met, go to
Step 3.
3. Adjust R51.
Note
Turning R51 clockwise decreases the RER
percentage; turning R51 counterclockwise
increases the RER percentage.
4. Return to the MAIN Menu and run another

cycle.
5. Observe the RBC RER in the RAW DATA
screen.
6. If the ratio is still outside of specification, repeat
this process until the RBC Editing Ratio is within
the specified value.
7. Return to the MAIN Menu and press [RUN] to
access the RUN screen.
8. Observe the RBC Histogram.
9. The trailing edge of the histogram should be
straight with almost no humps. Refer to Editing
Ratio Displays.
Continue with 1. Go to WBC Gain.
WBC Gain
Editing Ratio Displays
WBC Gain

Prepare Latex 1. From the [RUN] screen, press [SPECIMEN TYPE], then [PATIENT SPECIMEN].
Dilution 2. Press the touch plate to run a cycle (exits Gain Adjust Mode).
3. Obtain a clean container.
4. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice to
display the [10 mL DISPENSE] key.
5. Press [10 mL DISPENSE] four times to dispense 20 mL of diluent into the container.
Note
Each 10 mL dispense requires key to be pressed twice.
6. Add 1 drop of well mixed 5.0 µm latex particle solution into the 20 mL of diluent and mix
well.
7. Before entering the Gain Adjust Mode, use the prepared WBC latex dilution to fill the
Pre-Mix cup up to the level of the diluent inlet port.
8. From the MAIN Menu, press [RUN] followed by [SPECIMEN TYPE].
9. Press Shift and # (simultaneously) to activate the Gain Adjust Mode.
Note
The message <PREPARING FOR GAIN ADJ.> displays and WBC dilution is
transferred to the WBC Mixing Chamber. When the preparation cycle is complete, the
RUN screen displays.
Set WBC 1. Press the touch plate to run a cycle.

Gain
Note
Always return to the RUN screen to run a cycle.
Note
When the procedure is completed, or if there is a need to exit the Gain Adjust Mode
while performing this procedure, such as constant clogs or improper dilution ratio, press
[SPECIMEN TYPE] followed by [PATIENT SPECIMEN].
Note
The WBC count should be between 10.0 and 30.0. If the count is outside this range,
modify the dilution ratio of the latex particles, exit Gain Adjust Mode and try again.
2. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].

Note
The key changes to [SMOOTHING ON] and is highlighted, indicating the smoothing
function is ON.
4. Press [WBC HISTOGRAM].

5. Observe the lower part of the histogram data to determine if the channel number for
peak of 100 is Channel 56 ± 1 channel.
o If the specification is met, go to PLT Gain.
o If the specification is not met, adjust R22, and repeat Step 1 through Step 5.
Note
Turning R22 clockwise decreases the channel number; turning R22 counterclockwise
increases the channel number.
Continue with 1. Go to PLT Gain.

PLT Gain
WBC Gain Adjustments
PLT Gain
Prepare 1. From the [RUN] screen, press [SPECIMEN TYPE], Note

Latex then [PATIENT SPECIMEN]. The PLT channel cannot be correctly
2. Press the touch plate to run a cycle (exits Gain Adjust adjusted unless the RBC channel is at
Mode). the correct gain. The RBC channel
3. From the MAIN Menu, press [SPECIAL must be verified prior to adjusting the
PROTOCOLS] followed by [MORE] twice. Place a PLT channel.
clean container under the Sample Probe and press
[10 mL DISPENSE] four times to dispense 20 mL of
diluent.
Note
Each 10 mL dispense requires key to be pressed
twice.
4. Add one (1) drop of well-mixed 3.31 µm latex particle

solution and mix well.
5. Hold the diluted latex solution under the Sample Probe
and press [1/50 DILUTION] to aspirate for 1:50
dilution, while in the SPECIAL PROTOCOLS Menu.
6. Place a clean container under the Sample Probe and
press [1/50 DISPENSE] to dispense the 1:50 dilution.
7. Pull open Solenoid 2-5 (located directly under the left
side of the RBC/PLT Transducer) to drain the existing
solution in the RBC Mixing Chamber.
8. Pour the 1:50 dilution of 3.31 latex particles into RBC
Mixing Chamber.
Enter Gain 1. Pour 10 mL of diluent into the Pre-Mix Cup (do not add
Adjust any latex particle solution).
Mode 2. From the RUN screen, press [SPECIMEN TYPE].
3. Press Shift and # (simultaneously) to activate the
Gain Adjust Mode.
Note
The message <PREPARING FOR GAIN ADJ.>
displays and WBC dilution is transferred to the WBC
Mixing Chamber. When the preparation cycle is
complete, the RUN screen displays.
Note
When running PLT counts, the lower front cover must
be on the instrument to prevent noise from interfering
with PLT results.
Adjust PLT 1. Press the touch plate to run a cycle.

Gain
Note
The PLT count should be between 150 and 350. If the
count is outside this range, adjust the dilution ratio of
the latex particles by adding more dilution or latex.
2. From the MAIN Menu, press [DIAGNOSTICS]

followed by [MORE].
Note
The key changes to [SMOOTHING ON] and is
highlighted, indicating the smoothing function is ON.
4. Press [PLT HISTOGRAM].

5. Observe the lower part of the histogram data to
determine if the channel number for peak of 100 is
Channel 136 ± 1 channel (refer to PLT Gain
Adjustments (Example).).
o If the specification is met, the procedure is
complete.
o If the specification is not met, adjust R41.
Note
Turning R41 clockwise decreases the channel
number; turning R41 counterclockwise increases the
channel number.
6. Return to the RUN screen and run another cycle.

7. Repeat Step 2 through Step 5.
8. To exit Gain Adjust mode, go to the RUN Menu and
press [SPECIMEN TYPE] followed by [PATIENT
SPECIMEN]
9. Run two to three background cycles.
10.Verify acceptable background results (refer to
Specifications & Checklist).
PLT Gain Adjustments (Example)

VP-10 Diluent and Sample Verification/Adjustment
Purpose Verify diluent dispense to maintain proper dilution ratios. Module CELL-DYN 1800
Materials Required 10 mL Graduated Cylinder Time 00:15 min

40 µL Micropipettes
100 µL Micropipettes
Clean 50 mL Container
WARNING
Potential Biohazard
This procedure consist of both Diluent and Sample adjustment that can be accessed with the following links:
Diluent Volume Verification
Sample Volume Verification
To minimize problems like coincidence passage, the CELL-DYN 1800 uses two different dilution ratios of whole blood to
diluent. The ratio for WBC/HGB is 1:285; the ratio for RBC/MCV/PLT is 1:12,801. The following procedure is used to verify
the diluent dispense to maintain proper dilution ratios and thereby optimize instrument performance.
Diluent Volume Verification
Verify 1. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice.
Diluent 2. Place an empty 10 mL graduated cylinder under the Sample Probe and press [10 mL
Volume DISPENSE] twice to dispense 10 mL of diluent.
3. Verify a volume of 10 mL ± 0.2 mL.
4. Place an empty 10 mL graduated cylinder under Sample Probe and press [1/50
DILUTION]. Once the probe has returned to the aspirate position, press
[1/50 DISPENSE] to dispense.
5. Verify a volume of 5 mL ± 0.1 mL.
Note
The volume dispensed is under direct computer control. If the volume is out-of-range, the
Dispenser and Stepper Motor drive circuitry must be repaired. (Refer to RR-E1.02
Diluent Syringe Driver Assembly.)
Sample Volume Verification
Sample Volume 1. Remove the 1/32" silicone tubing attached to the top of the sample probe.
Verification 2. Attach a 100 microliter pipette to the silicone tubing.
3. Place the tip of the pipette on the bottom of a small container and press [10 mL
DISPENSE] twice.
Note
Keep the tip of the pipette submerged when dispensing.
4. Take the pipette out of the container and wipe any drop from the end of the pipette,
being careful not to wick any liquid from the end.
5. Press [1/50 DILUTION].
6. Verify that the column of liquid is no more than 1/16 inch above or below 100
microliter mark on the pipette.
7. Replace the 100 microliter pipette with a 40 microliter pipette.
8. Place the tip of the 40 microliter pipette on the bottom of a small container and press
[1/50 DISPENSE].
9. Take the pipette out of the container and wipe any drop from the end of the pipette,
being careful not to wick any liquid from the end.
10. Press [1/250 DILUTION].
11.Verify that the column of liquid is no more than 1/16" above or below 40 microliter
mark on the pipette.
Remove Pipette 1. Place a waste container under the tip of the pipette and press [1/250 DISPENSE].
2. Remove the pipette and re-attach the silicone tubing to the sample probe.
Note
The volume aspirated is under direct computer control. If the volume is out-of-range,
the Sample Syringe and Stepper Motor Drive must be repaired. (Refer to RR-E1.04
Sample Syringe Driver Assembly.)
VP-11 Stepper Motor Power Test and Verification
Purpose Verify stepper motor power. Module CELL-DYN 1800
Materials Required None Time 00:30 min
Test Stepper 1. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times.
Motor Power Then press [SERVICE DEC CODE].
2. Type in 128 from the keyboard and press Enter.
3. The test runs for approximately 30 seconds.
4. When the test is complete, the results are displayed on the screen.
5. Compare the results displayed on the screen with the nominal values listed in Motor
Power Specifications. Ensure that all values fall within the specified ranges.
Motor Power Specifications
VP-12 Sample Probe Alignment Check
Purpose Check Probe Alignment. Module CELL-DYN 1800
Materials Required Standard Set of Hex Wrenches Time 00:10 min
Check 1. From the MAIN Menu, press

Probe [DIAGNOSTICS], followed by [MORE]
Alignment twice, then [PROBE HOME].
2. Observe the wash block in relationship
to the probe tip. Verify that the tip of the
probe is between the waste removal
channel and the top of the countersink,
located at the bottom of the wash block.
Note
Proper probe tip location is essential for
proper cleaning of the probe, and
prevents carryover and CV problems.
3. If alignment is required, loosen the set

screw on the probe collar and raise or
lower probe until proper height is
achieved.
4. Retighten set screw on probe collar.
5. Press PROBE DOWN to exit.
VP-13 Serial Transmit to LIS Verification
Purpose Verify operation of serial port. Module CELL-DYN 1800
Materials Required Laptop computer w/Windows 2000 Time 00:40 min

Copy of CELL-DYN 1800 Interface Specification (Latest Revision)
Nine (9) Pin Null Modem Serial Cable
This verification procedure is used to verify the operation of the serial port (COM 1) that is used for communication with a
Laboratory Information System (LIS). This procedure only verifies the transmit function.
A 9 pin, "null modem", serial cable is needed for this procedure.
Note
The screens shown in this procedure are given for example only. Each model instrument has slightly different field
structure and content to the count data message that is downloaded. Consult the applicable interface specification
document for details.
Create New 1. Place the laptop near the instrument and

Connection connect the null modem serial cable to
COM 1 on the right side of the instrument.
Connect the other end to COM 1 on the
laptop.
2. From the Windows 2000 desktop, use the
mouse to click on Start.
3. Highlight Programs, Accessories,
Communications, and click Hyper
Terminal.
The Connection Description window

displays.
4. In the Name field, type in the filename

sertest1.
5. Select the first icon and click the OK
button.
Select 1. The Connect To window displays:

Connection 2. At the bottom, in the box Connect
using:, use the mouse to select COM 1.
Note
All the other boxes are blocked (gray).
3. Click the OK button.
Configure 1. The COM1 Properties window displays:

COM1 2. Configure the Bits per second: field to
match the Baud Rate in the CELL-DYN
SETUP menu.
3. Verify that the Data bits, Parity and Stop
bits are configured according to the
CELL-DYN SETUP menu.
4. In the CELL-DYN SETUP menu, verify
the CTS enabled setting:
o If CTS enabled is off set Flow
control: to Xon/Xoff.
o If CTS enabled is on set Flow
control: to Hardware.
5. Click the OK button.
sertest1 1. The test session window named sertest1

Active is now active.
Note
If the message "Unable to open COM1.
Please check your port settings"
displays, there may be another device
assigned to the COM1 port, such as the
modem. Contact the local area IS
administrator for assistance with resolving
device interrupt conflicts.
2. Compare the results to the interface

specification. The screen is shown for
example only. Transmit specimen data as
many times as desired.
Transmit 1. From the MAIN Menu, press

Data [DATALOG], followed by [TRANSMIT
DATA].
2. Enter starting and ending sequence
numbers of data to be transmitted.
3. Compare the results to the
CELL-DYN 1800 Interface Specification.
Note
The screen below is shown for example
only.
4. If desired, click on File then Print to

obtain a printout. When testing is
complete, click on File then Save. After
saving, click on Call, then Disconnect,
then exit the window by clicking the X.
Close out the Hyper Terminal window by
clicking the X.
VP-14 Hard Disk Drive Setup and Verification
Purpose Provide instructions to verify/modify CMOS Settings, partition and format Module CELL-DYN 1800
replacement hard disk drives
Materials DK, Drive, Hard, IDE (8200570701) Time 00:30 min

Required DKS (C) SRV ABBOTT CD3200 (8938070001)
CELL-DYN 1800 Installation Disk
Preparation Note
If replacing an existing customer hard disk drive, print copies of all customer
settings, quality control data files, and the data log if the drive is accessible. It
may not be possible to access this data if the hard disk drive is corrupt or
defective.
1. Power OFF the system.

2. Verify jumper setting is set to Master according to Jumper Configuration Table
on hard disk drive.
Install Hard Drive 1. Install the new hard drive using the existing hardware and cables (refer to
RR-F1.04 Hard Disk Drive).
Access CMOS Setup 1. Power ON the analyzer and press the Delete key, when prompted, to display
Utility Screen the CMOS Setup Utility Screen.
Verify CMOS Settings: 1. Use the up/down arrow keys to select the STANDARD CMOS SETUP.
Standard CMOS Setup 2. Press the Enter key.
3. Set Pri Master to Auto.
4. Set 32 Bit Mode to ON.
Note
Pri Slave, Sec Master and Sec Slave show Not Installed.
Verify CMOS Settings: 1. Use the up/down arrow keys to select the ADVANCED CMOS SETUP.
Advanced CMOS Setup 2. Press the Enter key.
3. Set Quick Boot to Enabled.
4. Set 1st Boot Device to Floppy.
5. Set 2nd Boot Device to IDE-0.
6. Set 3rd Boot Device to Disabled.
7. Set 4th Boot Device to Disabled.
Verify CMOS Settings: 1. Use the up/down arrow keys to select the ADVANCED CHIPSET SETUP.
Advanced Chipset 2. Press the Enter key.
Setup 3. Set LCD CRT Selection to Simultaneous.
4. Set LCD Type to Type 8.
Verify CMOS Settings: 1. Use the up/down arrow keys to select the POWER MANAGEMENT SETUP.
Power Management 2. Press the Enter key.
Setup 3. Set Power Management/APM to Disabled.
4. Set CPU Critical Temperature to 60°C/140°F.
Verify CMOS Settings: 1. Use the up/down arrow keys to select the PERIPHERAL SETUP.
Peripheral Setup 2. Press the Enter key.
3. Set OnBoard FDC to Enabled.
Save CMOS Settings 1. Place the DKS (C) SRV ABBOTT CD3200 (8938070001) (service disk) into
and Exit CMOS Setup the floppy drive.
2. Press F10.
The message Save current settings and exit (Y/N)? displays.
3. Type Y and press Enter.
Note
Saves the CMOS settings, exits the CMOS Setup Utility and restarts the
computer.
Create DOS Partition 1. At the A:\> prompt, type FDISK and press Enter.
2. At the FDISK Options menu, enter choice 1 and press Enter.
3. At the Create DOS Partition or Logical DOS Drive menu enter choice 1 and
press Enter.
Note
If you get the message Primary DOS Partition already exists, press Esc to
exit. Then go to Format Hard Drive, Step 1.
The message Do you wish to use the maximum available size for a
Primary DOS Partition and make the partition active (Y/N)? displays.
4. Type Y, and press Enter.
Note
Uses the maximum size for the hard drive DOS partition and makes it active.
5. When the screen displays System will now restart. Insert DOS system
diskette in drive A:. Press any key when ready, press Enter.
The system reboots and displays the A:\ prompt.
Format Hard Drive 1. At the A:\> prompt, type FORMAT C: /S and press Enter.
The message WARNING: ALL DATA ON NON-REMOVABLE DISK DRIVE

C: WILL BE LOST! Proceed with Format (Y/N)? displays.
2. Type Y, and press Enter.
The message Volume label (11 characters, ENTER for none)? displays.
3. Press Enter for None.
Install DOS and System 1. At the A:\> prompt, type INSTALL and press Enter.
Software
Note
Installs DOS and system files.
2. When the system installation is complete, remove the service disk and insert
the CELL-DYN 1800 Installation disk into the floppy drive.
3. Type Install and press Enter.
Note
When the installation is complete, the message "Power off the instrument
and remove diskette. Power on the instrument." displays.
4. Remove the CELL-DYN 1800 Installation disk and power OFF the instrument.
Verification 1. Power ON the instrument

2. Verify that the system boots up properly to the application software.
Note
During initial power up after a hard disk drive installation, DO NOT turn off the
instrument while the Data Log is being created. This corrupts the Data Log.
3. If printouts are available, re-enter the data from the printouts. If not, perform
instrument calibration procedure and verify control recovery.
VP-15 Software Installation/Upgrades
Purpose Install or Upgrade Software Module CELL-DYN 1800
Materials Required Disk, installation (8938144001) Time 00:10 min
Software 1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.

Installation/Upgrade 2. Press ESC on the PC keyboard. A warning message displays on the screen.
3. Press Alt and x (simultaneously). The following DOS prompt displays:
C:\CD1800>.
4. Insert the CELL-DYN 1800 Installation disk into Drive A.
5. Type a: and press Enter.
6. Type install and press Enter.
Note
When the installation is complete, the message "Power off the instrument
and remove diskette. Power on the instrument." displays.
7. Remove the diskette and power OFF the instrument.

8. Power ON the instrument and allow it to initialize.
Note
During initial power up after a hard disk drive installation, DO NOT turn off the
instrument while the Data Log is being created. This corrupts the Data Log.

Abbott: CELL-DYN 1800 Automated Hematology Analyzer Service & Support Manual

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Abbott: CELL-DYN 1800 Automated Hematology Analyzer Service & Support Manual

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ABBOTT

• Flow Panel System [3]

Fluid Power Supply

Reagent Inlet Panel

Syringe Drive Assembies

Electronics Card Cage

LCD Display Screen and Keyboard

Power Supply Assembly

Major Subsystem Descriptions

• Data Interface and Control Subsystem

Data Interface and Control Subsystem

• DLA/CCM (Data Link Adapter/Cell Count Module)

Data Interface and Control Block Diagram

See the figure below for a diagram of the measurement process.

Measurement Block Diagram

Solenoid, Motor Drive, and Pump Subsystem

Solenoid, Motor Drive and Pump Block Diagram

• Single Board Computer PCB

User Interface Computer

SBC (Single Board Computer) PCB

DLA (Data Link Adapter)

• Receives measurement and analyzer status data from the CCM

Serial I/O Ports

COM1 RS-232 Data Output

Parallel I/O Port

Touch Pad (Membrane) Keyboard

LCD Display Screen (Color)

The LCD Display Screen has the following characteristics:

• Size: 8.5 x 6.4 inches (10.4 inches measured diagonally)

Hard Disk Drive

AC and DC Power Distribution Subsystem

+28VDC Switching Power Supply

Pre-amplifier Module (PAM)

PAM PCB Diagram

Signal Processor Module (SPM)

RBC and PLT Signals

There are two modes for RBC/PLT data acquisition:

Cell Count Module (CCM)

• 16 bit address bus

Scratch Pad RAM

This RAM is used for general purpose programming.

This RAM is used for histogram building.

Histogram Building (cell counting)

The following sequence is executed to build histograms:

1. Histogram Memory is cleared.

This disconnects Histogram Memory from the processor buss.

3. Wait for an End of Conversion (EOC) signal from SPM.

7. The count is incremented and stored back into histogram memory.

The CDM port is connected directly to microprocessor ports E, G and K.

Cable Distribution Module

Solenoid, Motor Drive and Pump Block Diagram

Solenoid Driver Module (SDM)

Motor Processor Module (MPM)

Control functions of the MPM are performed by microprocessor (U5).

Stepper Drive Printed Circuit Board

Pressure/Vacuum Regulator Module

Power Supply Assembly

Pump Relay Module (PRM)

Refer to Solenoid, Motor Drive and Pump Block Diagram.

Single Board Computer (SBC)

Data Link Adapter (DLA)

Jumpers W1 - W6 assign the DLA interrupt to one of the following:

* Since IRQ 5 is used by the DLA, W1 is hard-wired (this is the default).