PHARMACEUTICAL TOXICOLOGY

EXPERIMENT 8: Data Sheet

CHORIOALLANTOIC MEMBRANE (CAM) ASSAY

Name: Date Performed:

Member Number: Group Number: Year and Section:

ILLUSTRATION
After administration of the test substance:
Test substance Image Number of vessels
Negative control: 32
Sterile water

Test substance: 74
Dimethylsulfoxide (DMSO)

CAM Vascularity of test substance: ________________

Computation:

ASSESSMENT AND CONCLUSION:

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PHARMACEUTICAL TOXICOLOGY

EXPERIMENT 8: Post-Lab Questions

CHORIOALLANTOIC MEMBRANE (CAM) ASSAY

Name: Date Performed:

Seat Number: Group Number: Year and Section:

1. What is the reason of using sterilized filter paper in CAM Assay?

2. What is the difference between in-ovo and ex-ovo types of CAM Assay?

3. Describe the proper placement of the test substance to the chicken egg. Where should
you place the sterilized filter paper, on the tip or at the bottom of an egg?

4. What is the role of chorioallantoic membrane?

5. Is CAM Assay enough for the assessment on the carcinogenicity of a certain drug or
compound?

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