Abacus5 Fluidics v2.

1
SV in Needle Position

V6 V7 V8

Open 4diff
from Waste
Diff-5p Lyse Dilu II Air
Dilu I V16/1 Dilu 1
Puffer Puffer Puffer Puffer Baso Dilu
P Dilu 3 clean
V5 4diff in from V20
Laser Temperature Lyse2
V3 WBC 4diff out
Baso Control 4diff in
Diluent Inlet System
1 2 Diff-5p
Green Dilu 4
3
to V38/1 to V39/1 V4
Diff-5p Open 3
SV inner SV outer 1 2 3

Inlet Lyse Air Diluent ring ring

Inlet
2 1
Orange Puffers
Yellow to V36 V40 V42 V37 V41 V43 /1 Mea V16
Puff Sheath Dilu 7
Puffer
Dilu 6 Dilu 2
V35
from WBC RBC MIX
aperture
P Mix Sample
MIX 1 2 3

from RBC V20 Dilu 5

from V19 Mix RBC
aperture
Sample WBC Lyse 1 V19
V34 V1
1 M R W
Mea to V1/1
3

i B B
2

Puff V32 x C to V35 C

WBC
Small Laser to V34
Preheat

V12
1 2
Dilu 8 Dilu 9
3 Air2
Baso
Air1/1 Air1/2 P
V23 V2
laser

V15 Cham
V9 V10 V11 Puff
2

4diff
3

Waste
1
2

V13 V14
3

3
1

Pump2 Pump1

Dilu 10

Needle

Baso Dilu
Dilu 5 V24
clean
Dilu 2 1 2
Dilu 7
V33 V18 3 Lyse 1 Lyse 2
1 2
1 2
3
Dilu 4 Dilu 10
3
V44 1 2
Dilu 6 V28
1 2
V25
Dilu 1 V17
3 1 2 Dilu 3
3
3
V30
1 2
Dilu 8 1 2
3 V26
1 2
V29 Dilu 9
3
Diff-5p 3
V43 Air2 1 2
Air1/2
to Dilu 1 2 to Dilu 1V362 V39 3
Puffer Puffer to V38 1 2 V40
3 3 V37 1 2 V42
to Dilu 1 2 Diff-5p to Lyse 3 to Dilu
1 2
V41 to Dilu 1 2 Air1/1
Puffer 3 Puffer 3 Puffer Puffer 3 to Dilu 1 2
Puffer 3
Puffer 3

D D D
i D i
i L D
l i f D D
l y i
u l f i i
u s l
u 5 l l
e u
p u u
M
M
M
M
D1-MDilu D2-MStop
D3-MLyse D4-MAir