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«2 United States Patent Bickerton et al. ‘USO1OL30701B2 (10) Patent No.: (54) @ORONAVIRUS (72) Inventors: Erica Bickerton, Woking (GB), Sarah Keep, Woking (GB): Paul Briton, Woking (GB) (73) Assignee: THE PIRBRIGHT INSTITUTE, Woking, Pirbright (GB) (9) Notice: Subject to any disclaimer, the tem ofthis patent is extended or adjusted under 35, USC. 154(b) by 0 days. (21) Appl. Nos 18928179 (22) PCT Filed: Jul. 23, 2018 (86) PCTNo: — PCT/GB2018/082124 $371 EL, @) Date” Jan. 23, 2017 (87) PCT Pub. No WO2016/012793 PCT Pub. Date: Jan. 28, 2016 «sy Prior Publication Data US 201710216427 Al Aug. 3, 2017 co) Foreign Application Priority Data Jul, 23, 2014 (GB) 14130207 (1) Intech AGIK 392218 (200601) cRN 700 (2006.01) CRN 9/2 (200601) 61K 39100 200601) (2) US. cre AGIK 39/215 (2013.01); CN 700 (2013.01), C12N 9/127 201300; CRY 20707048 (2013.01); AGIK 2039/5254 (2013.01): 461K 2039/54 (2013.01), CIN 277020021 (2013.01); C12N 2770/2002: (2013 01); C12N 2770/20034 (2013.01); C12N 2770220051 (2013.01); C12N 2770/20062 (2013.01) (S8)_Fleld of Classification Search cee AGIK 39/215 ‘Seo application file for complete search history 6) References Cited U.S. PATENT DOCUMENTS 7452,$42 B2* 11/2008 Denison co7k 1av0s “4721 FOREIGN PATENT DOCUMENTS WO Wo-2nodp2s50 42 10.2004 WO Wo2sostisis A2 "62005 WO Woorim AT 42007 WO Woot OSI4G AL L201 (OTHER PUBLICATIONS Spemy Jounal of Virology 2005, vol 79, No.6, pp. 3891-3400: Altschul etal, Basi local alignment search ol. Mol. Biol 215 405-10 (999) AAmmayapepan tal, Kenifcaion of saqunce changes respon: She forthe atleaaion of avian ifections bronchitis vis sain Arkansas DP, Arch, Vira. 154(3)495.9 (2009). ‘Anonsmows “EM SIDKES77S77", Oct. 30, 2013, ‘Ammesto eal, A rcombiaant avian infectious bronchide vius ‘xpresing a hserlogous spike gene belonging othe 491 serotype, Pros One, 6)e24882 COIN). Armest etal, The replicase gene of avian coronvinus infectious ‘bronchitis vinis is a determinant of pathogenicity. PLoS One 4110 67384 (2009). ‘Armesto ea, Transient doin’ selection for the madication ‘an generation of recombinant nfeciows bronchitis coromavinaes, Methods Mol. Bis 454 255-73 (208). ‘Ausubel etal, Short Protocols ia Molecular Biolog th ton, Chaper 18 (1999) Brion

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