By GS 2013EC

By GS 2013EC
By GS 2013EC
By GS 2013EC
By GS 2013EC
By GS 2013EC
By GS 2013EC
By GS 2013EC

The purity of a pharmaceutical preparation of sulfanilamide, C6H4N2O2S, can

be determined by oxidizing the sulfur to SO2 and bubbling the SO2 through
H2O2 to produce H2SO4. The acid is then titrated with a standard solution of
NaOH to the bromothymol blue end point, where both of sulfuric acid’s acidic
protons have been neutralized. Calculate the purity of the preparation, given
that a 0.5136-g sample required 48.13 mL of 0.1251 M NaOH.
SOLUTION
Conservation of protons for the titration reaction requires that
2 moles H2SO4 = moles NaOH
Since all the sulfur in H2SO4 comes from sulfanilamide, we use a conservation
of mass on sulfur to establish the following stoichiometric relationship.
Moles C6H4N2O2S = moles H2SO4
Combining the two conservation equations gives a single equation relating the
moles of analyte to the moles of titrant.
2 moles C6H4N2O2S = moles NaOH
Making appropriate substitutions for the moles of sulfanilamide and moles of
NaOH gives
2*g sulfanilamide

MbVb
Fw sulfanilamide

The amount of protein in a sample of cheese is determined by a Kjeldahl

analysis for nitrogen. After digesting a 0.9814-g sample of cheese, the nitrogen
is oxidized to NH4
+, converted to NH3 with NaOH, and distilled into a
collection flask containing 50.00 mL of 0.1047 M HCl. The excess HCl is then
back titrated with 0.1183 M NaOH, requiring 22.84 mL to reach the
bromothymol blue end point. Report the %w/w protein in the cheese given
that there is 6.38 g of protein for every gram of nitrogen in most dairy
products.
SOLUTION
In this procedure, the HCl reacts with two different bases; thus
Moles HCl = moles HCl reacting with NH3 + moles HCl reacting with NaOH
Conservation of protons requires that
Moles HCl reacting with NH3 = moles NH3
Moles HCl reacting with NaOH = moles NaOH
A conservation of mass on nitrogen gives the following equation.
Moles NH3 = moles N
Combining all four equations gives a final stoichiometric equation of
Moles HCl = moles N + moles NaOH
Making appropriate substitutions for the moles of HCl, N, and NaOH gives
which we solve for the grams of nitrogen.
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By GS 2013EC