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  • ATCC Medium 30-2006

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    Mario Alfredo Loeza Cabrera
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    The document lists the formulation for DMEM:F-12 Medium, including inorganic salts, vitamins, amino acids, and other components. It provides the specific amounts in grams per liter of each component, such as calcium chloride, copper sulfate, iron nitrate, magnesium sulfate, sodium bicarbonate, and various vitamins and amino acids needed for the cell culture medium. The medium also contains glucose, HEPES, linoleic acid, and other additives. The formulation is provided by ATCC (American Type Culture Collection).

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    The document lists the formulation for DMEM:F-12 Medium, including inorganic salts, vitamins, amino acids, and other components. It provides the specific amounts in grams per liter of each component, such as calcium chloride, copper sulfate, iron nitrate, magnesium sulfate, sodium bicarbonate, and various vitamins and amino acids needed for the cell culture medium. The medium also contains glucose, HEPES, linoleic acid, and other additives. The formulation is provided by ATCC (American Type Culture Collection).

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    12 views1 page

    ATCC Medium 30-2006

    Uploaded by

    Mario Alfredo Loeza Cabrera
    The document lists the formulation for DMEM:F-12 Medium, including inorganic salts, vitamins, amino acids, and other components. It provides the specific amounts in grams per liter of each component, such as calcium chloride, copper sulfate, iron nitrate, magnesium sulfate, sodium bicarbonate, and various vitamins and amino acids needed for the cell culture medium. The medium also contains glucose, HEPES, linoleic acid, and other additives. The formulation is provided by ATCC (American Type Culture Collection).

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    © All Rights Reserved
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    Formulation for DMEM:F-12 Medium

    ATCC® 30-2006

    Inorganic Salts (g/liter) Vitamins (g/liter)


    CaCl2 (anhydrous) 0.11665 D-Biotin 0.00000365
    CuSO4 (anhydrous) 0.0000008 Choline Chloride 0.00898
    Fe(NO3)3·9H2O 0.00005 Folic Acid 0.00265
    FeSO4·7H2O 0.000417 myo-Inositol 0.01261
    MgSO4 (anhydrous) 0.08495 Niacinamide 0.00202
    KCl 0.3118 D-Pantothenic Acid 0.00224
    NaHCO3 1.20000 (hemicalcium)
    NaCl 7.00000 Pyridoxine·HCl 0.00203
    Na2HPO4 (anhydrous) 0.07100 Riboflavin 0.00022
    NaH2PO4 ·H2O 0.06250 Thiamine·HCl 0.00217
    ZnSO4·7H2O 0.000432 Vitamin B-12 0.00068

    Amino Acids (g/liter)


    Other (g/liter)
    L-Alanine 0.00445
    L-Arginine·HCl 0.14750 D-Glucose 3.15100
    L-Asparagine·H2O 0.00750 HEPES 3.57480
    L-Aspartic Acid 0.00665 Hypoxanthine 0.00239
    L-Cystine·HCl·H2O 0.01756 Linoleic Acid 0.000044
    L-Cystine·2HCl 0.03129 Phenol Red, Sodium Salt 0.00810
    L-Glutamic Acid 0.00735 Putrescine·2HCl 0.00008
    L-Glutamine 0.36510 Pyruvic Acid·Na 0.05500
    Glycine 0.01875 DL-Thioctic Acid 0.000105
    L-Histidine·HCl·H2O 0.03148 Thymidine 0.000365
    L-Isoleucine 0.05437
    L-Leucine 0.05895
    L-Lysine·HCl 0.09135
    L-Methionine 0.01724
    L-Phenylalanine 0.03548
    L-Proline 0.01725
    L-Serine 0.02625
    L-Threonine 0.05355
    L-Tryptophan 0.00902
    L-Tyrosine·2Na·2H2O 0.05582
    L-Valine 0.05285

    ATCC (American Type Culture Collection) 800-638-6597 or 703-365-2700


    P.O. Box 1549 Fax: 703-365-2750
    Manassas, VA 20108 USA E-mail: tech@atcc.org
    www.atcc.org Or contact your local distributor
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