9012 sv Australian Standard™ Laboratory glassware— One-mark pipettes AS 2166—2002This Australian Standard was prepared by Committee CH-001, Laboratory Glassware and Related Apparatus. It was approved on behalf of the Council of Standards Australia on 15 November 2002 and published on 11 December 2002 The following are represented on Committee CH-001: Australian Chamber of Commerce and Industry National Association of Testing Authorities Australia National Standards Commission Royal Australian Chemical Institute Royal College of Pathologists of Australia Science Industry Inc Keeping Standards up-to-date Standards are living documents which reflect progress in science, technology and systems. To maintain their currency, all Standards are periodically reviewed, and new editions are published. Between editions, amendments may be issued. 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This Standard was issued in draft form for comment as DR 01234.AS 2166—2002 Australian Standard™ Laboratory glassware— One-mark pipettes Originated as AS RI6— 1053. Previous edtlon AS 21661996, ‘Thad eaition 2002 COPYRIGHT © Standards Ausvala Inemational AN rights ave reserved. No part of this work may be reproduced or copied in any form or by any ‘means, eloctronic or mechanical, including photocopying, without the wrtton permission ofthe publisher, Published by Standards Australia International Li GPO Box 5420, Sydney, NSWV 2001, Australia sen 0 73974028 3AS 2166-2002 PREFACE This Standard was prepared by the Standards Australia Committee CH-001, Laboratory Glassware and Related Apparatus to supersede AS 2166—1995, Laboratory glassware— One-mark pipettes. This Standard is based on but not equivalent to ISO 648:1977, Laboratory glassware—One- ‘mark pipettes (reconfirmed 1999). This edition is essentially the same as the 1995 edition but has been reformatted for clarity and Clause 7.1 considering delivery time has been re- written, The objective of this Standard is to provide a specification for one-mark pipettes required for general use in laboratoriesAS 21662002 CONTENTS SCOPE... REFERENCED DOCUMENTS 4 DEFINITIONS ....00c:sunsunesnnnsnnannnntnsinstnnineneinein 4 NOMINAL CAPACITIES AND TOLERANCES ON CAPACITIES, 4 CONSTRUCTION 5 6 9 0 0 GRADUATION LINE DELIVERY TIME.... MARKING COLOUR CODING...AS 2166-2002 4 STANDARDS AUSTRALIA Australian Standard Laboratory glassware— One-mark pipettes 1 SCOPE This Standard sets out requirements for one-mark pipettes, suitable for general laboratory purposes. Two classes of accuracy are specified. Class A pipettes being of higher accuracy than Class B pipettes, NOTE: The method of verification and notes for the use of one-mark pipettes are given in AS 2162. 2 REFERENCED DOCUMENTS The following documents are referred to in this Standard: AS 2162 Verification and use of volumetric apparatus 2162.1 Part I: General—Volumetrie glassware Iso 1769 Laboratory glassware—Pipettes—Colour coding 3. DEFINITIONS For the purpose of this Standard, the definitions below apply 3.1 Capacity The volume of water at 20-C, expressed tres, delivered by the pipette at 20-C, when it is filled to the graduation line and emptied in accordance with the method given in AS 2162.1 3.2. Delivery time The duration of descent of the water meniscus from the graduation line to the point at which it appears to come to rest in the jet, 3.3 Waiting time The time allowed, after the delivery time, to ensure that the total amount of liquid for which the pipette is calibrated has been delivered. 4 NOMINAL CAPACITIES AND TOLERANCES ON CAPACITIES ‘The nominal capacities and tolerances on the capacities of one-mark pipettes shall be as specified in Table | Standards Australis wun standards.com.auAS 2166—2002 TABLE 1 NOMINAL CAPACITIES AND TOLERANCES OF ONE-MARK PIPETTES al capacity Tolerance on capac Class A os 40.008 1 20.008 2 0.01 3 40.015 5 20015 10 40.02 1s 20,025 2» 40.08 25 40.03 50 20.05 100 40.08 200 01 5 CONSTRUCTION 5.1 Material ‘The pipettes shall be constructed of glass having suitable chemical and thermal properties and shall be as free as possible from visible defects. 5.2 Shape 5.2.1 0.5 mL pipettes Pipettes of 0.5 mL capacity shall consist of a straight tube with a jet at the lower end. $2.2 I mil. pipettes and Class mL. pipentes Pipettes of | mL capacity and, in Class B, of 2 mL capacity, may be of similar shape to that specified in Clause 5.2.1. Alternatively, they may be of the bulb type as specified in Clause 5.2.3 5.2.3. Other pipettes Class A pipettes having a capacity of 2 mL, and all larger pipettes, shall consist of a bulb with suction and delivery tubes, the three segments being straight and coaxial. The general shape of the pipettes shall be that shown in Figure 1 5.24 Safety bulb If a safety bulb is provided, it should be of approximately spherical form, situated betwee the graduation line and the top of the pipette, in accordance with the dimensions given in Table 3 53 ensions ‘The dimensions of one-mark pipettes shall conform to the dimensions given in Tables 2 and www. standards.com.au © Standards Australia,AS 2166-2002 6 54 Fil h of top of pipette The top of the pipette shall be finished square with the axis and shall be free from any blemishes which might interfere with the required accurate control in the setting of the meniscus. The end may be lightly fire-polished or smoothly ground with a slight bevel on the outside. 5.5 Bulb The main portion of the bulb in I mL and 2 mL capacity pipettes shall be either eylinérical or oval. All other pipeties shall have cylindrical bulbs. 5.6 Delivery jet 5.6.1 Construction of jet The delivery jet shall be strongly constructed with a smooth and gradual taper and without any sudden constriction at the orifice. NOTE: 1 A fire-polished finish reduces the danger of chipping in use, but should not result in constriction (2s indicated in Clause 5.6.1) oF in undue stress 2A suitable shape for the jet is shown in Figure 2. 5.6.2. Finish of jet end The end of the jet shall be finished by one of the following methods, listed in order of preference: (a) Smoothly ground, square with the axis, slightly bevelled on the outside, and fire-polished, (>) Smoothly ground, square with the axis, and slightly bevelled on the outside. 6 GRADUATION LINE ‘The graduation line shall be a clean, permanent line of uniform thickness extending completely around the tube, except that a gap not exceeding 10 percent of the cireumference is permitted. The thickness of the line shall not exceed 0.2 mm © Standards Austialia worm standards.com 2u7 AS 2166—2002 TABLE 2 DIMENSIONS OF ONE-MARK PIPETTES, Length | Length External | Diameter | Diameter | Diameter Nominal of of | diameter oftube | ofbutb+ | of safety xen suction | delivery | at (straight | (max) | bth pac tube | tabe® | graduation tube | pattern (mas) (ein | taming | tines Get mm) | pipettes) (max) (max) Bulb] Straight pattern | pattern mt_| pipette | pipette o> — 280 = = 30 = 30 = = 1 328 280 iso | 110 3.0 50 60 ° ° 2 | 350 280 iso | 125 35 55 70 9 9 3 | 380 iso | 138 35 60 n s | a0 = iso | 14s 40 65 = 2 wo | 480 too | 160 4s 65 16 1s | 480 - io | 200 50 65 20 20 | 320 1m | 210 35 70 2 as | 330 170 | 220 33 70 4 so | 550 170 | 230 60 15 25 100 | 600 - 170 | 240 75 80 - 25 200_| 650 io_| 340 85 9.0 25 7 Applicable only to bulb pattem pipettes + For straight pattem pipettes; maximum internal diameter. For bulb pattern pipettes; maximum internal diamcier of suetion tube. TABLE 3 DIMENSIONS APPLYING TO ALL CAPACITIES Distanoe of graduation line from top of 100 pipette (min. Distance from graduation line to top oF 10 bulb (min.) Distance from graduation line to tip of 120 Jet (straight patter pipettes) (min.} Wall thickness Bull pipettes of 1 ml. and 2 malin.) 07 Other pipettes (min) 10 Distance from top of pipette (0 top of 30 safety bull (min) bulb 10 15 Distance from bottom of safety _graluation line (rin) wow. standards.com.au © Standards AuetraliaAS 2166—2002 8 27 FIGURE 1 BULB PATTERN FOR ONE-MARK PIPETTES, FIGURE 2. SUITABLE SHAPE FOR JET OF ONE-MARK PIPETTE, © Standards Australia www. standards. com.au° AS 2166—2002 7 DELIVERY TIME 7.1. General Delivery is made without restrieting or increasing the natural rate of outflow. The delivery ime, determined with the pipette in a vertical position and with the receiving vessel slightly is in contact with the inside of the vessel but without shown in Table 4 nelined, so that the tip of the movement of one against the other, shall be within the limi The delivery times specified in Table 4 relate to the use of volumetric pipettes with water. When used with liquids possessing density and/or viscosity significantly different from those of water, the delivery times may be different. TABLE 4 DELIVERY TIMES FOR ONE-MARK PIPETTES USING WATER Delivery times s is Class A Clase B Nominatenpacity | X. waiting time | Waiting time 154 | No waiting time specified specified mL min, max. | min. | max, ‘min. max. 08 4 3 20 1 5 9 ey 2 5 9 25 3 = - a3 3 1 n 30 10 8 2 40 13 ~ - 0 » 9 3 ° 50 2 0 13 10 50 30 B 18 B 6 100 25 30 25 60 200 40 0 7.2. Waiting time The receiving vessel shall be withdrawn after a waiting period of approximately 3 s, or specified waiting time where applicable, which shall commence when the meniscus comes to rest slightly above the lower end of the jet. The the jet shall not be added to the delivered volume, nall quantity of liquid which remains in Where no specific waiting time is nominated, a waiting time of approximately 3 s shall be observed to ensure that delivery is complete before removal of the pipette from the receiving vessel. NOTE: It is unnecessary to adhere closely to the period of 3 s: itis sufficient to be certain that the meniscus has come to rest in the jet before removal of the pipette from contact with the receiving vessel In the case of pipettes where a waiting time of 15s is specified, this period shall be observed before removing the pipette from the receiving vessel. NOTE: Pipettes of Class A accuracy may either be (a) without definite waiting time, in which case a waiting period of approximately 3s is observed; or (b) with a waiting time of 15 s. Pipettes of Class B accuracy should be without definite waiting time as described under Item (a), www. standards.com.au © Standards Australia,AS 2166-2002 0 7.3 Correspondence of observed and marked delivery times Clause 9.2(b) shall both be within the limits given in Table 4, The maximum permissible difference between observed and marked delivery times shall be as follows (a) For 0.5, 1,2 and 3 mL pipettes (>) For 5, 10 and 15 ml pipettes (©) For 20 and 25 mL pipettes 4s, (A) For 50, 100 and 200 mL. pipeties.... co oo oe 5s. 8 MARKING 8.1 Mandatory inseriptions The following inscriptions shall be permanently and legibly marked on all one-mark pipettes: (a) A number to indicate the nominal capacity. (>) Adjacent to the number, the symbol ‘mL.’ to indicate the unit of graduation. © (d)__ ‘The inscription “A” or *B’, to indicate the class of accuracy for which the pipette has been graduated. (©) The waiting time, if specified, in the form: "Ex + 15 (£) ‘The maker's and/or vendor's name or mark, NOTE: Manufacturers making statement of compliance with this Australian Standard on a product, packaging, or promotional material related to that product are advised to ensure that such compliance is capable of being verified 8.2 Class A pipettes The following additional inscriptions should be marked on Class A pipettes intended for official verification or certification: they should preferably be marked on other Class A pipettes and may if desired also be marked on Class B pipettes: (a) An identification number. (b) The delivery time, in seconds, 8.3 Leg The graduation line and all figures and inscriptions shall be of such size and shape as to be clearly legible under normal conditions of use. of graduation line and marking, 9 COLOUR CODING Colour coding, if used on the pipettes, shall conform to ISO 1769. standards Australie wun standards.com.uAS 21662002AS 2166-2002 R NorEs‘Standards Australia ‘Standards Australia is an independent company, limited by guarantee, which preperes and publishes most of the voluntary technical and commercial standards used in Australia. These standards are developed through an open process of consultation and concensus, in which all interested partios are invited to participate. Through a Memorandum of Understanging with the Commonwealth government, ‘Standards Australia Is recognized as Australia's peak national standards body. 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