Pubmed Boneadhesi Set

PMID- 25861724
OWN - NLM
STAT- MEDLINE
DCOM- 20160912
LR - 20181113
IS - 1872-8294 (Electronic)
IS - 0169-409X (Print)
IS - 0169-409X (Linking)
VI - 94
DP - 2015 Nov 1
TI - Biomaterial strategies for engineering implants for enhanced osseointegration
and
bone repair.
PG - 53-62
LID - S0169-409X(15)00048-4 [pii]
LID - 10.1016/j.addr.2015.03.013 [doi]
AB - Bone tissue has a remarkable ability to regenerate and heal itself. However,
large
bone defects and complex fractures still present a significant challenge to
the
medical community. Current treatments center on metal implants for structural
and
mechanical support and auto- or allo-grafts to substitute long bone defects.
Metal
implants are associated with several complications such as implant loosening
and
infections. Bone grafts suffer from donor site morbidity, reduced
bioactivity, and
risk of pathogen transmission. Surgical implants can be modified to provide
vital
biological cues, growth factors and cells in order to improve
osseointegration and
repair of bone defects. Here we review strategies and technologies to
engineer metal
surfaces to promote osseointegration with the host tissue. We also discuss
strategies for modifying implants for cell adhesion and bone growth via
integrin
signaling and growth factor and cytokine delivery for bone defect repair.
CI - Copyright © 2015 Elsevier B.V. All rights reserved.
FAU - Agarwal, Rachit
AU - Agarwal R
AD - Woodruff School of Mechanical Engineering, Georgia Institute of Technology,
Atlanta,
GA, USA; Petit Institute for Bioengineering and Bioscience, Georgia Institute
of
Technology, Atlanta, GA, USA.
FAU - García, Andrés J
AU - García AJ
AD - Woodruff School of Mechanical Engineering, Georgia Institute of Technology,
Atlanta,
GA, USA; Petit Institute for Bioengineering and Bioscience, Georgia Institute
of
Technology, Atlanta, GA, USA. Electronic address:
andres.garcia@me.gatech.edu.
LA - eng
GR - R01 AR062920/AR/NIAMS NIH HHS/United States
PT - Journal Article
PT - Research Support, N.I.H., Extramural
PT - Review
DEP - 20150408
TA - Adv Drug Deliv Rev
JT - Advanced drug delivery reviews
JID - 8710523
RN - 0 (BMP2 protein, human)
RN - 0 (Biocompatible Materials)
RN - 0 (Bone Morphogenetic Protein 2)
RN - 0 (Cytokines)
RN - 0 (Intercellular Signaling Peptides and Proteins)
RN - 0 (Polymers)
SB - IM
MH - Biocompatible Materials
MH - Bone Morphogenetic Protein 2/pharmacology
MH - Bone Regeneration/drug effects/physiology
MH - Bone-Implant Interface/physiology
MH - Cytokines/metabolism
MH - Drug Delivery Systems/*methods
MH - Humans
MH - Intercellular Signaling Peptides and Proteins/metabolism
MH - Osseointegration/*drug effects/physiology
MH - Osteogenesis/*drug effects/physiology
MH - Polymers/pharmacology
MH - Surface Properties
MH - Tissue Scaffolds
PMC - PMC4598264
MID - NIHMS678998
OTO - NOTNLM
OT - Coatings
OT - Growth factor
OT - Metal implants
OT - Polymer Hydrogel
OT - Therapeutic release
EDAT- 2015/04/12 06:00
MHDA- 2016/09/13 06:00
CRDT- 2015/04/12 06:00
PHST- 2014/09/05 00:00 [received]
PHST- 2015/02/08 00:00 [revised]
PHST- 2015/03/17 00:00 [accepted]
PHST- 2015/04/12 06:00 [entrez]
PHST- 2015/04/12 06:00 [pubmed]
PHST- 2016/09/13 06:00 [medline]
AID - S0169-409X(15)00048-4 [pii]
AID - 10.1016/j.addr.2015.03.013 [doi]
PST - ppublish
SO - Adv Drug Deliv Rev. 2015 Nov 1;94:53-62. doi: 10.1016/j.addr.2015.03.013.
Epub 2015
Apr 8.
PMID- 23948983
OWN - NLM
STAT- MEDLINE
DCOM- 20140930
LR - 20181202
IS - 0341-2695 (Print)
IS - 0341-2695 (Linking)
VI - 37
IP - 12
DP - 2013 Dec
TI - Role of mesenchymal stem cells in bone regeneration and fracture repair: a
review.
PG - 2491-8
LID - 10.1007/s00264-013-2059-2 [doi]
AB - Mesenchymal stem cells (MSCs) are non-haematopoietic stromal stem cells that
have
many sources, such as bone marrow, periosteum, vessel walls, adipose, muscle,
tendon, peripheral circulation, umbilical cord blood, skin and dental

tissues. They
are capable of self-replication and of differentiating into, and contributing
to the
regeneration of, mesenchymal tissues, such as bone, cartilage, ligament,
tendon,
muscle and adipose tissue. The homing of MSCs may play an important role in
the
repair of bone fractures. As a composite material, the formation and growth
of bone
tissue is a complex process, including molecular, cell and biochemical
metabolic
changes. The recruitment of factors with an adequate number of MSCs and the
micro-environment around the fracture are effective for fracture repair.
Several
studies have investigated the functional expression of various chemokine
receptors,
trophic factors and adhesion molecules in human MSCs. Many external factors
affect
MSC homing. MSCs have been used as seed cells in building tissue-engineered
bone
grafts. Scaffolds seeded with MSCs are most often used in tissue engineering
and
include biotic and abiotic materials. This knowledge provides a platform for
the
development of novel therapies for bone regeneration with endogenous MSCs.
FAU - Wang, Xin
AU - Wang X
AD - Key Laboratory of Peoples Liberation Army, Institute of Orthopedics, Chinese
PLA
General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, 100853,
People's
Republic of China.
FAU - Wang, Yu
AU - Wang Y
FAU - Gou, Wenlong
AU - Gou W
FAU - Lu, Qiang
AU - Lu Q
FAU - Peng, Jiang
AU - Peng J
FAU - Lu, Shibi
AU - Lu S
LA - eng
PT - Research Support, Non-U.S. Gov't
PT - Review
DEP - 20130815
TA - Int Orthop
JT - International orthopaedics
JID - 7705431
SB - IM
MH - Bone Regeneration/*physiology
MH - Cell Differentiation/physiology
MH - Fractures, Bone/*therapy
MH - Humans
MH - Mesenchymal Stem Cell Transplantation/*methods
MH - Mesenchymal Stem Cells/cytology
MH - Tissue Engineering
MH - Wound Healing/physiology
PMC - PMC3843208
EDAT- 2013/08/21 06:00
MHDA- 2014/10/01 06:00
CRDT- 2013/08/17 06:00
PHST- 2013/06/12 00:00 [received]
PHST- 2013/07/26 00:00 [accepted]
PHST- 2013/08/17 06:00 [entrez]
PHST- 2013/08/21 06:00 [pubmed]
PHST- 2014/10/01 06:00 [medline]
AID - 2059 [pii]
AID - 10.1007/s00264-013-2059-2 [doi]
PST - ppublish
SO - Int Orthop. 2013 Dec;37(12):2491-8. doi: 10.1007/s00264-013-2059-2. Epub 2013
Aug
15.
PMID- 27871408
OWN - NLM
STAT- MEDLINE
DCOM- 20170426
LR - 20200728
IS - 1083-7515 (Linking)
VI - 21
IP - 4
DP - 2016 Dec
TI - The Biology of Bone and Ligament Healing.
PG - 739-761
LID - S1083-7515(16)30072-9 [pii]
LID - 10.1016/j.fcl.2016.07.017 [doi]
AB - This review describes the normal healing process for bone, ligaments, and
tendons,
including primary and secondary healing as well as bone-to-bone fusion. It
depicts
the important mediators and cell types involved in the inflammatory,
reparative, and
remodeling stages of each healing process. It also describes the main
challenges for
clinicians when trying to repair bone, ligaments, and tendons with a specific
emphasis on Charcot neuropathy, fifth metatarsal fractures, arthrodesis, and

tendon
sheath and adhesions. Current treatment options and research areas are also
reviewed.
CI - Copyright © 2016 Elsevier Inc. All rights reserved.
FAU - Cottrell, Jessica A
AU - Cottrell JA
AD - Department of Biological Sciences, Seton Hall University, 400 South Orange
Avenue,
South Orange, NJ 07101, USA. Electronic address: cottreje@shu.edu.
FAU - Turner, Jessica Cardenas
AU - Turner JC
AD - Department of Biomedical Engineering, New Jersey Institute of Technology, 323
Martin
Luther King Boulevard, Newark, NJ 07102, USA.
FAU - Arinzeh, Treena Livingston
AU - Arinzeh TL
AD - Department of Biomedical Engineering, New Jersey Institute of Technology, 323
Martin
Luther King Boulevard, Newark, NJ 07102, USA.
FAU - O'Connor, J Patrick
AU - O'Connor JP
AD - Department of Orthopaedics, Rutgers-New Jersey Medical School, Medical
Sciences
Building, Room E-659, 185 South Orange Avenue, Newark, NJ 07103, USA.
LA - eng
GR - R01 DE019926/DE/NIDCR NIH HHS/United States
PT - Review
PL - United States
TA - Foot Ankle Clin
JT - Foot and ankle clinics
JID - 9615073
SB - IM
MH - Arthrodesis
MH - Fracture Healing/physiology
MH - Fractures, Bone/*physiopathology
MH - Humans
MH - Ligaments/injuries/*physiopathology
MH - Osteogenesis/physiology
MH - Tendon Injuries/*physiopathology
MH - Tendons/physiopathology
MH - Wound Healing/*physiology
OTO - NOTNLM
OT - *Arthrodesis
OT - *Bone fracture healing
OT - *Charcot neuropathy
OT - *Fifth metatarsal fractures
OT - *Tendon and ligament healing
OT - *Tissue regeneration
OT - *Wound healing
EDAT- 2016/11/23 06:00
MHDA- 2017/04/27 06:00
CRDT- 2016/11/23 06:00
PHST- 2016/11/23 06:00 [entrez]
PHST- 2016/11/23 06:00 [pubmed]
PHST- 2017/04/27 06:00 [medline]
AID - S1083-7515(16)30072-9 [pii]
AID - 10.1016/j.fcl.2016.07.017 [doi]
PST - ppublish
SO - Foot Ankle Clin. 2016 Dec;21(4):739-761. doi: 10.1016/j.fcl.2016.07.017.
PMID- 29785024
OWN - NLM
STAT- MEDLINE
DCOM- 20190513
LR - 20201030
IS - 1546-170X (Electronic)
IS - 1078-8956 (Print)
IS -
1078-8956 (Linking)
VI -
24
IP -
6
DP -
2018 Jun
TI -
Targeting skeletal endothelium to ameliorate bone loss.
PG -
823-833
LID -
10.1038/s41591-018-0020-z [doi]
AB -
Recent studies have identified a specialized subset of CD31(hi)endomucin(hi)
(CD31(hi)EMCN(hi)) vascular endothelium that positively regulates bone
formation.
However, it remains unclear how CD31(hi)EMCN(hi) endothelium levels are
coupled to
anabolic bone formation. Mice with an osteoblast-specific deletion of Shn3,
which
have markedly elevated bone formation, demonstrated an increase in
CD31(hi)EMCN(hi)
endothelium. Transcriptomic analysis identified SLIT3 as an osteoblast-
derived,
SHN3-regulated proangiogenic factor. Genetic deletion of Slit3 reduced
skeletal
CD31(hi)EMCN(hi) endothelium, resulted in low bone mass because of impaired
bone
formation and partially reversed the high bone mass phenotype of Shn3(-/-)
mice.
This coupling between osteoblasts and CD31(hi)EMCN(hi) endothelium is
essential for
bone healing, as shown by defective fracture repair in SLIT3-mutant mice and
enhanced fracture repair in SHN3-mutant mice. Finally, administration of
recombinant
SLIT3 both enhanced bone fracture healing and counteracted bone loss in a
mouse
model of postmenopausal osteoporosis. Thus, drugs that target the SLIT3
pathway may
represent a new approach for vascular-targeted osteoanabolic therapy to treat
bone
loss.
FAU - Xu, Ren
AU - Xu R
AUID- ORCID: 0000-0001-6578-4553
AD - Department of Pathology and Laboratory Medicine, Cornell University, New
York, NY,
USA.
FAU - Yallowitz, Alisha
AU - Yallowitz A
York, NY,
USA.
FAU - Qin, An
AU - Qin A
AD - Department of Orthopaedics, Shanghai Key Laboratory of Orthopaedic Implant,
Shanghai
Ninth People's Hospital, Shanghai Jiaotong University School of Medicine,
Shanghai,
China.
FAU - Wu, Zhuhao
AU - Wu Z
AD - Laboratory of Brain Development and Repair, The Rockefeller University, New
York,
NY, USA.
FAU - Shin, Dong Yeon
AU - Shin DY
York, NY,
USA.
FAU - Kim, Jung-Min
AU - Kim JM
AD - Division of Rheumatology, Department of Medicine, University of Massachusetts
Medical School, Worcester, MA, USA.

FAU - Debnath, Shawon
AU - Debnath S
York, NY,
USA.
FAU - Ji, Gang
AU - Ji G
AD - Research Division, Hospital for Special Surgery, New York, NY, USA.
AD - Department of Joint Surgery, The Third Hospital of Hebei Medical University,
Shijiazhuang, China.
FAU - Bostrom, Mathias P
AU - Bostrom MP
AD - Division of Adult Reconstruction and Joint Replacement, Department of
Orthopaedic
Surgery, Hospital for Special Surgery, New York, NY, USA.
FAU - Yang, Xu
AU - Yang X
FAU - Zhang, Chao
AU - Zhang C
AD - Institute for Computational Biomedicine, Cornell University, New York, NY,
USA.
FAU - Dong, Han
AU - Dong H
AD - Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute
and
Harvard University Medical School, Boston, MA, USA.
AD - Department of Medicine, Harvard Medical School and Brigham and Women's
Hospital,
Boston, MA, USA.
FAU - Kermani, Pouneh
AU - Kermani P
AD - Division of Regenerative Medicine, Department of Medicine, Ansary Stem Cell
Institute, Cornell University, New York, NY, USA.
FAU - Lalani, Sarfaraz
AU - Lalani S
York, NY,
USA.
FAU - Li, Na
AU - Li N
York, NY,
USA.
FAU - Liu, Yifang
AU - Liu Y
York, NY,
USA.
FAU - Poulos, Michael G
AU - Poulos MG
FAU - Wach, Amanda
AU - Wach A
AD - Department of Biomechanics, Hospital for Special Surgery, New York, NY, USA.
FAU - Zhang, Yi
AU - Zhang Y
York, NY,
USA.
FAU - Inoue, Kazuki
AU - Inoue K
AD - Arthritis and Tissue Degeneration Program and David Z. Rosensweig Genomics
Research
Center, Hospital for Special Surgery, New York, NY, USA.
AD - Department of Medicine, Weill Cornell Medical College, Cornell University,
New York,
NY, USA.
FAU - Di Lorenzo, Annarita
AU - Di Lorenzo A
York, NY,
USA.
FAU - Zhao, Baohong
AU - Zhao B
AD - Arthritis and Tissue Degeneration Program and David Z. Rosensweig Genomics
Research
Center, Hospital for Special Surgery, New York, NY, USA.
AD - Department of Medicine, Weill Cornell Medical College, Cornell University,
New York,
NY, USA.
FAU - Butler, Jason M
AU - Butler JM
FAU - Shim, Jae-Hyuck
AU - Shim JH
AUID- ORCID: 0000-0002-4947-3293
AD - Division of Rheumatology, Department of Medicine, University of Massachusetts
Medical School, Worcester, MA, USA.

FAU - Glimcher, Laurie H
AU - Glimcher LH
AD - Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute
and
Harvard University Medical School, Boston, MA, USA.
laurie_glimcher@dfci.harvard.edu.
AD - Department of Medicine, Harvard Medical School and Brigham and Women's
Hospital,
Boston, MA, USA. laurie_glimcher@dfci.harvard.edu.
FAU - Greenblatt, Matthew B
AU - Greenblatt MB
York, NY,
USA. mag3003@med.cornell.edu.
LA - eng
GR - DP5 OD021351/OD/NIH HHS/United States
GR - S10 OD019986/OD/NIH HHS/United States
GR - R01 HL126913/HL/NHLBI NIH HHS/United States
DEP - 20180521
TA - Nat Med
JT - Nature medicine
JID - 9502015
RN - 0 (DNA-Binding Proteins)
RN - 0 (Emcn protein, mouse)
RN - 0 (Membrane Proteins)
RN - 0 (Nerve Tissue Proteins)
RN - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN - 0 (Receptors, Immunologic)
RN - 0 (Recombinant Proteins)
RN - 0 (Schnurri-3 protein, mouse)
RN - 0 (Sialoglycoproteins)
RN - 0 (Slit3 protein, mouse)
RN - 0 (roundabout protein)
SB - IM
CIN - Nat Rev Rheumatol. 2018 Jul;14(7):386. PMID: 29875380
CIN - Nat Rev Endocrinol. 2018 Sep;14(9):508-509. PMID: 30050157
MH - Animals
MH - Bone Marrow Cells/drug effects/metabolism
MH - Bone Resorption/diagnostic imaging/*pathology
MH - Bone and Bones/diagnostic imaging/drug effects/*pathology
MH - DNA-Binding Proteins/deficiency/metabolism
MH - Disease Models, Animal
MH - Endothelium/drug effects/*pathology
MH - Fracture Healing/drug effects
MH - Humans
MH - Membrane Proteins/metabolism
MH - Mice, Inbred BALB C
MH - Mice, Inbred C57BL
MH - Neovascularization, Physiologic/drug effects
MH - Nerve Tissue Proteins/metabolism
MH - Osteoblasts/drug effects/metabolism/pathology
MH - Osteogenesis/drug effects
MH - Osteoporosis, Postmenopausal/drug therapy/pathology
MH - Ovariectomy
MH - Platelet Endothelial Cell Adhesion Molecule-1/metabolism
MH - Receptors, Immunologic/metabolism
MH - Recombinant Proteins/administration & dosage/pharmacology/therapeutic use
MH - Sialoglycoproteins/metabolism
PMC - PMC5992080
MID - NIHMS953996
COIS- Conflicts of Interest LHG is on the board of directors of and holds equity in
the
GlaxoSmithKline and Waters Corporations. She is also a founder of Quentis
Pharmaceuticals.
EDAT- 2018/05/23 06:00
MHDA- 2019/05/14 06:00
CRDT- 2018/05/23 06:00
PHST- 2017/06/30 00:00 [received]
PHST- 2018/03/22 00:00 [accepted]
PHST- 2018/05/23 06:00 [pubmed]
PHST- 2019/05/14 06:00 [medline]
PHST- 2018/05/23 06:00 [entrez]
AID - 10.1038/s41591-018-0020-z [pii]
AID - 10.1038/s41591-018-0020-z [doi]
PST - ppublish
SO - Nat Med. 2018 Jun;24(6):823-833. doi: 10.1038/s41591-018-0020-z. Epub 2018
May 21.
PMID- 31037624
OWN - NLM
STAT- MEDLINE
DCOM- 20190808
LR - 20210313
IS - 0065-2598 (Print)
IS - 0065-2598 (Linking)
VI - 1132
DP - 2019
TI - Periostin in Bone Regeneration.
PG - 49-61
LID - 10.1007/978-981-13-6657-4_6 [doi]
AB - Bone regeneration is an efficient regenerative process depending on the
recruitment
and activation of skeletal stem cells that allow cartilage and bone formation
leading to fracture consolidation. Periosteum, the tissue located at the

outer
surface of bone is now recognized as an essential player in the bone repair
process
and contains skeletal stem cells with high regenerative potential. The matrix
composition of the periosteum defines its roles in bone growth, in cortical

bone
modeling and remodeling in response to mechanical strain, and in bone repair.
Periostin is a key extracellular matrix component of the periosteum involved

in
periosteum functions. In this chapter, we summarize the current knowledge on
the
bone regeneration process, the role of the periosteum and skeletal stem
cells, and
Periostin functions in this context. The matricellular protein Periostin has
several
roles through all stages of bone repair: in the early days of repair during
the
initial activation of stem cells within periosteum, in the active phase of
cartilage
and bone deposition in the facture callus, and in the final phase of bone
bridging
and reconstitution of the stem cell pool within periosteum.
FAU - Duchamp de Lageneste, Oriane
AU - Duchamp de Lageneste O
AD - INSERM UMR1163, Imagine Institute, Paris Descartes University, Paris, France.
FAU - Colnot, Céline
AU - Colnot C
AD - INSERM UMR1163, Imagine Institute, Paris Descartes University, Paris, France.
celine.colnot@inserm.fr.
LA - eng
PT - Review
PL - United States
TA - Adv Exp Med Biol
JT - Advances in experimental medicine and biology
JID - 0121103
RN - 0 (Cell Adhesion Molecules)
RN - 0 (POSTN protein, human)
MH - *Bone Regeneration
MH - Cartilage/physiology
MH - Cell Adhesion Molecules/*physiology
MH - Humans
MH - Osteogenesis
MH - Periosteum/*physiology
MH - Stem Cells/cytology
OTO - NOTNLM
OT - Bone repair
OT - Periosteum
OT - Periostin
OT - Stem cell
EDAT- 2019/05/01 06:00
MHDA- 2019/08/09 06:00
CRDT- 2019/05/01 06:00
PHST- 2019/05/01 06:00 [entrez]
PHST- 2019/05/01 06:00 [pubmed]
PHST- 2019/08/09 06:00 [medline]
AID - 10.1007/978-981-13-6657-4_6 [doi]
PST - ppublish
SO - Adv Exp Med Biol. 2019;1132:49-61. doi: 10.1007/978-981-13-6657-4_6.
PMID- 30966948
OWN - NLM
STAT- MEDLINE
DCOM- 20200529
LR - 20200529
IS - 1742-5689 (Print)
IS - 1742-5662 (Linking)
VI - 16
IP - 153
DP - 2019 Apr 26
TI - Adhesive-based tendon-to-bone repair: failure modelling and materials
selection.
PG - 20180838
LID - 10.1098/rsif.2018.0838 [doi]
LID - 20180838
AB - Surgical reattachment of tendon to bone is a procedure marked by high failure
rates.
For example, nearly all rotator cuff repairs performed on elderly patients
with
massive tears ultimately result in recurrence of tearing. These high failure
rates
have been attributed to stress concentrations that arise due to the
mechanical
mismatch between tendon and bone. Although recent studies have identified
potential
adhesives with mechanical properties tuned to alleviate these stress
concentrations,
and thereby delay the onset of failure, resistance to the progression of
failure has
not been studied. Here, we refined the space of adhesive material properties
that
can improve surgical attachment by considering the fracture process. Using
cohesive
zone modelling and physiologically relevant values of mode I and mode II
adhesive
fracture toughnesses, we predicted the maximum displacement and strength at
failure
of idealized, adhesively bonded tendon-to-bone repairs. Repair failure
occurred due
to excessive relative displacement of the tendon and bone tissues for strong
and
compliant adhesives. The failure mechanism shifted to rupture of the entire
repair
for stiffer adhesives below a critical shear strength. Results identified a
narrow
range of materials on an Ashby chart that are suitable for adhesive repair of
tendon
to bone, including a range of elastomers and porous solids.
FAU - Avgoulas, Evangelos I
AU - Avgoulas EI
AD - 1 Department of Engineering, University of Cambridge , Trumpington Street,
Cambridge
CB2 1PZ , UK.
FAU - Sutcliffe, Michael P F
AU - Sutcliffe MPF
AD - 1 Department of Engineering, University of Cambridge , Trumpington Street,
Cambridge
CB2 1PZ , UK.
FAU - Linderman, Stephen W
AU - Linderman SW
AD - 2 Department of Orthopaedic Surgery, Washington University School of Medicine
, St
Louis, MO 63131 , USA.
FAU - Birman, Victor
AU - Birman V
AD - 3 Missouri Science and Technology Global-St Louis, and Department of
Mechanical and
Aerospace Engineering , St Louis, MO 63131 , USA.
FAU - Thomopoulos, Stavros
AU - Thomopoulos S
AD - 4 Department of Orthopedic Surgery, Columbia University , New York, NY
10032 , USA.
AD - 5 Department of Biomedical Engineering, Columbia University , New York, NY
10032 ,
USA.
FAU - Genin, Guy M
AU - Genin GM
AD - 6 NSF Science and Technology Center for Engineering Mechanobiology,
Department of
Mechanical and Aerospace Engineering, Washington University , St Louis, MO
63130 ,
USA.
LA - eng
GR - T32 AR060719/AR/NIAMS NIH HHS/United States
GR - F30 AR069491/AR/NIAMS NIH HHS/United States
GR - U01 EB016422/EB/NIBIB NIH HHS/United States
TA - J R Soc Interface
JT - Journal of the Royal Society, Interface
JID - 101217269
RN - 0 (Adhesives)
SB - IM
MH - *Adhesives
MH - Animals
MH - *Biocompatible Materials
MH - Biomechanical Phenomena
MH - Bone and Bones/*injuries
MH - Humans
MH - Models, Biological
MH - Stress, Mechanical
MH - Tendon Injuries/*surgery
MH - Tendons/*pathology
MH - Wound Healing
PMC - PMC6505561
OTO - NOTNLM
OT - *cohesive zone model
OT - *enthesis
OT - *rotator cuff
COIS- Although we have no competing interests, we have applied for US patents on
technology related to the approach studied in this article.
EDAT- 2019/04/11 06:00
MHDA- 2020/05/30 06:00
CRDT- 2019/04/11 06:00
PHST- 2019/04/11 06:00 [entrez]
PHST- 2019/04/11 06:00 [pubmed]
PHST- 2020/05/30 06:00 [medline]
AID - rsif20180838 [pii]
AID - 10.1098/rsif.2018.0838 [doi]
PST - ppublish
SO - J R Soc Interface. 2019 Apr 26;16(153):20180838. doi: 10.1098/rsif.2018.0838.
PMID- 32181262
OWN - NLM
STAT- PubMed-not-MEDLINE
LR - 20200928
IS - 2297-1769 (Print)
IS - 2297-1769 (Linking)
VI - 7
DP - 2020
TI - Biomechanical Testing of a Calcium Phosphate-Phosphoserine-Based Mineral-
Organic
Adhesive for Non-invasive Fracture Repair of Mandibular Fractures in Dogs.
PG - 59
LID - 10.3389/fvets.2020.00059 [doi]
LID - 59
AB - Mandibular fracture repair is complicated by limited availability of bone as
well as
the presence of the neurovascular bundle and an abundance of tooth roots.
Fractures
at the location of the mandibular first molar teeth are common and it can be
particularly challenging to apply stable fixation. Non-invasive fracture
repair
techniques utilize intraoral placement of fixation devices typically
involving
polymerized composites and/or interdental wiring. A novel calcium
phosphate-phosphoserine-based mineral-organic adhesive was tested ex vivo to
determine its effects on augmenting strength of different non-invasive
fracture
fixation techniques. This study both tested the use of mineral-organic
adhesive for
the purpose of stabilizing currently used non-invasive fracture repair
constructs
(intraoral composite splinting ± interdental wiring) and evaluated adhesive
alone or
with subperiosteally placed plates on buccal cortical bone surface. Aside
from
controls, not receiving an osteotomy along the mesial root of the mandibular
first
molar tooth, six treatment groups were tested to evaluate ultimate strength,
stiffness, angular displacement, bending moment, and application time. All
forms of
fixation were found to be significantly weaker than control (p < 0.001). Only
the
control (p < 0.001) and mineral-organic adhesive and composite (P = 0.002)
groups
were found to be significantly stronger than wire and composite. No
difference was
noted in stiffness between any groups with control or wire and composite.
Application times varied from the mineral-organic adhesive group (mean = 206
s) to
mineral-organic adhesive and composite (mean = 1,281 s). Twenty-three
fixation
devices exhibited adhesive failure, 20 demonstrated cohesive failure, and 5
failed
by cohesive and adhesive failure. When evaluating the ultimate strength of
the
fixation device groups, mineral-organic adhesive, and composite was shown to
be the
strongest construct. The use of resorbable bone adhesive and composite may
provide a
stronger fixation construct over interdental wire and composite for
mandibular
fracture repair in dogs.
CI - Copyright © 2020 Geddes, Thatcher, Hetzel, McCabe, Vandereby and Snyder.
FAU - Geddes, Alexander T
AU - Geddes AT
AD - Veterinary Dentistry and Oral Surgery, Department of Surgical Sciences,
School of
Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, United
States.
FAU - Thatcher, Graham P
AU - Thatcher GP
School of
States.
FAU - Hetzel, Scott
AU - Hetzel S
AD - Department of Biostatistics and Medical Informatics, University of
Wisconsin-Madison, Madison, WI, United States.
FAU - McCabe, Ronald P
AU - McCabe RP
AD - Department of Orthopedics and Rehabilitation, University of Wisconsin-
Madison,
Madison, WI, United States.
FAU - Vandereby, Ray Jr
AU - Vandereby R Jr
AD - Department of Orthopedics and Rehabilitation, University of Wisconsin-
Madison,
Madison, WI, United States.
FAU - Snyder, Christopher J
AU - Snyder CJ
School of
States.
LA - eng
DEP - 20200227
TA - Front Vet Sci
JT - Frontiers in veterinary science
JID - 101666658
PMC - PMC7058112
OTO - NOTNLM
OT - adhesive
OT - dogs
OT - fracture
OT - mandible
OT - non-invasive
OT - repair
OT - stiffness bone healing
OT - strength
EDAT- 2020/03/18 06:00
MHDA- 2020/03/18 06:01
CRDT- 2020/03/18 06:00
PHST- 2019/11/29 00:00 [received]
PHST- 2020/01/24 00:00 [accepted]
PHST- 2020/03/18 06:00 [entrez]
PHST- 2020/03/18 06:00 [pubmed]
PHST- 2020/03/18 06:01 [medline]
AID - 10.3389/fvets.2020.00059 [doi]
PST - epublish
SO - Front Vet Sci. 2020 Feb 27;7:59. doi: 10.3389/fvets.2020.00059. eCollection
2020.
PMID- 29215204
OWN - NLM
STAT- MEDLINE
DCOM- 20190306
LR - 20200930
IS - 0935-9648 (Linking)
VI - 30
IP - 4
DP - 2018 Jan
TI - Titanium Fiber Plates for Bone Tissue Repair.
LID - 10.1002/adma.201703608 [doi]
AB - Titanium plates are widely used in clinical settings because of their high
bone
affinity. However, owing to their high elastic modulus, these plates are not
suitable for bone repair since their proximity to the bone surface for
prolonged
periods can cause stress shielding, leading to bone embrittlement. In
contrast,
titanium fiber plates prepared by molding titanium fibers into plates by
simultaneously applying compression and shear stress at normal room
temperature can
have an elastic modulus similar to that of bone cortex, and stress shielding
will
not occur even when the plate lies flush against the bone's surface. Titanium
fibers
can form a porous structure suitable for cell adhesion and as a bone repair
scaffold. A titanium fiber plate is combined with osteoblasts and shown that
the
titanium fiber plate is better able to facilitate bone tissue repair than the
conventional titanium plate when implanted in rat bone defects. Capable of

being
used in close contact with bone for a long time, and even capable of
promoting bone
repair, titanium fiber plates have a wide range of applications, and are
expected to
make great contributions to clinical management of increasing bone diseases,
including bone fracture repair and bone regenerative medicine.
CI - © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Takizawa, Takashi
AU - Takizawa T
AD - Department of Orthopaedic Surgery, Shinshu University School of Medicine,
Asahi
3-1-1, Matsumoto, 390-8621, Japan.
FAU - Nakayama, Noboru
AU - Nakayama N
AD - Mecganicl Systems Engineering, Shinshu University Faculty of Engineering,
Wakasato
4-17-1, Nagano, 380-8553, Japan.
FAU - Haniu, Hisao
AU - Haniu H
AD - Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge
Research, Shinshu University, Matsumoto, 390-8621, Japan.

FAU - Aoki, Kaoru
AU - Aoki K
AD - Department of Applied Physical Therapy, Shinshu University School of Health
Sciences, Asahi 3-1-1, Matsumoto, 390-8621, Japan.
FAU - Okamoto, Masanori
AU - Okamoto M
Asahi
FAU - Nomura, Hiroki
AU - Nomura H
Asahi
FAU - Tanaka, Manabu
AU - Tanaka M
Asahi
FAU - Sobajima, Atsushi
AU - Sobajima A
Asahi
FAU - Yoshida, Kazushige
AU - Yoshida K
Asahi
FAU - Kamanaka, Takayuki
AU - Kamanaka T
Asahi
FAU - Ajima, Kumiko
AU - Ajima K

FAU - Oishi, Ayumu
AU - Oishi A
Asahi
FAU - Kuroda, Chika
AU - Kuroda C
Asahi

FAU - Ishida, Haruka
AU - Ishida H

FAU - Okano, Satomi
AU - Okano S

FAU - Kobayashi, Shinsuke
AU - Kobayashi S
Asahi
FAU - Kato, Hiroyuki
AU - Kato H
Asahi
FAU - Saito, Naoto
AU - Saito N
AUID- ORCID: 0000-0001-6424-087X
LA - eng
DEP - 20171207
PL - Germany
TA - Adv Mater
JT - Advanced materials (Deerfield Beach, Fla.)
JID - 9885358
RN - 0 (titanium fiber)
RN - D1JT611TNE (Titanium)
SB - IM
MH - Bone Plates
MH - Bone and Bones
MH - Porosity
MH - Titanium/*chemistry
OTO - NOTNLM
OT - biomaterials
OT - bone tissue repair
OT - plate
OT - regenerative medicine
OT - titanium fibers
EDAT- 2017/12/08 06:00
MHDA- 2019/03/07 06:00
CRDT- 2017/12/08 06:00
PHST- 2017/06/28 00:00 [received]
PHST- 2017/09/13 00:00 [revised]
PHST- 2017/12/08 06:00 [pubmed]
PHST- 2019/03/07 06:00 [medline]
PHST- 2017/12/08 06:00 [entrez]
AID - 10.1002/adma.201703608 [doi]
PST - ppublish
SO - Adv Mater. 2018 Jan;30(4). doi: 10.1002/adma.201703608. Epub 2017 Dec 7.
PMID- 31984560
OWN - NLM
STAT- MEDLINE
DCOM- 20201215
LR - 20201215
IS - 0935-9648 (Linking)
VI - 32
IP - 10
DP - 2020 Mar
TI - Bioactive Pore-Forming Bone Adhesives Facilitating Cell Ingrowth for Fracture
Healing.
PG - e1907491
LID - 10.1002/adma.201907491 [doi]
AB - The effectiveness of commercial bone adhesives is known to be hampered by the
weak
efficacy of cell ingrowth. The strategy of macropore-forming, especially
bioactive
macropores, holds considerable promise to circumvent this problem, thereby
promoting
fracture healing. Herein, a class of bioactive glass-involved macropore-
embedded
bone adhesives is developed, which is capable of facilitating the migration
of
bone-derived mesenchymal stromal cells into the adhesive layer and
differentiation
into osteocytes. The integration of bioactive glass-particle-encapsulated
porogens
in the bone adhesives is key to this approach. A robust instant bonding on
the bone
adhesive and a high efficiency of bone regeneration on a mouse skull are
observed,
both of which are vital for clinical applications and personalized surgical
procedures. This work represents a general strategy to design biomaterials
with high
cell-ingrowth efficacy.
FAU - Xu, Liju
AU - Xu L
AD - Beijing National Laboratory for Molecular Sciences, State Key Laboratory of
Polymer
Physics and Chemistry, CAS Research/Education Center for Excellence in
Molecular
Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing,
100190,
China.
AD - School of Chemical Sciences, University of Chinese Academy of Sciences,
Beijing,
100190, China.
FAU - Gao, Shan
AU - Gao S
AD - Department of Orthopedics, Peking University Third Hospital, Beijing, 100191,
China.
FAU - Zhou, Rubing
AU - Zhou R
China.
FAU - Zhou, Fang
AU - Zhou F
China.
FAU - Qiao, Yan
AU - Qiao Y
AUID- ORCID: 0000-0003-1069-7756
Polymer
Molecular
100190,
China.
Beijing,
100190, China.
FAU - Qiu, Dong
AU - Qiu D
Polymer
Molecular
100190,
China.
Beijing,
100190, China.
LA - eng
GR - 2017YFC1103300/National Basic Research Program/
GR - 21474122/National Natural Science Foundation of China/
GR - XDB12020300/Strategic Priority Research Program of the Chinese Academy of
Sciences/
GR - 1000 Young Talents program of China/
DEP - 20200127
PL - Germany
TA - Adv Mater
JID - 9885358
RN - 0 (Adhesives)
RN - 0 (Bioglass)
SB - IM
MH - Adhesives/*therapeutic use
MH - Animals
MH - Biocompatible Materials/*therapeutic use
MH - Bone Regeneration
MH - Cell Proliferation
MH - Cells, Cultured
MH - Ceramics/*therapeutic use
MH - *Fracture Healing
MH - Mice
MH - Osteogenesis
MH - Porosity
MH - Skull/*injuries/pathology/physiopathology
OTO - NOTNLM
OT - bioactive glass
OT - biomaterials
OT - bone adhesives
OT - bone healing
OT - macropores
EDAT- 2020/01/28 06:00
MHDA- 2020/12/16 06:00
CRDT- 2020/01/28 06:00
PHST- 2019/11/14 00:00 [received]
PHST- 2019/12/18 00:00 [revised]
PHST- 2020/01/28 06:00 [pubmed]
PHST- 2020/12/16 06:00 [medline]
PHST- 2020/01/28 06:00 [entrez]
AID - 10.1002/adma.201907491 [doi]
PST - ppublish
SO - Adv Mater. 2020 Mar;32(10):e1907491. doi: 10.1002/adma.201907491. Epub 2020
Jan 27.
PMID- 32528290
OWN - NLM
LR - 20200928
IS - 1663-9812 (Print)
IS - 1663-9812 (Linking)
VI - 11
DP - 2020
TI - The Bone Extracellular Matrix in Bone Formation and Regeneration.
PG - 757
LID - 10.3389/fphar.2020.00757 [doi]
LID - 757
AB - Bone regeneration repairs bone tissue lost due to trauma, fractures, and
tumors, or
absent due to congenital disorders. The extracellular matrix (ECM) is an
intricate
dynamic bio-environment with precisely regulated mechanical and biochemical
properties. In bone, ECMs are involved in regulating cell adhesion,
proliferation,
and responses to growth factors, differentiation, and ultimately, the
functional
characteristics of the mature bone. Bone ECM can induce the production of new
bone
by osteoblast-lineage cells, such as MSCs, osteoblasts, and osteocytes and
the
absorption of bone by osteoclasts. With the rapid development of bone
regenerative
medicine, the osteoinductive, osteoconductive, and osteogenic potential of
ECM-based
scaffolds has attracted increasing attention. ECM-based scaffolds for bone
tissue
engineering can be divided into two types, that is, ECM-modified biomaterial
scaffold and decellularized ECM scaffold. Tissue engineering strategies that
utilize
the functional ECM are superior at guiding the formation of specific tissues
at the
implantation site. In this review, we provide an overview of the function of
various
types of bone ECMs in bone tissue and their regulation roles in the behaviors
of
osteoblast-lineage cells and osteoclasts. We also summarize the application
of bone
ECM in bone repair and regeneration. A better understanding of the role of
bone ECM
in guiding cellular behavior and tissue function is essential for its future
applications in bone repair and regenerative medicine.
CI - Copyright © 2020 Lin, Patil, Gao and Qian.
FAU - Lin, Xiao
AU - Lin X
AD - Laboratory for Bone Metabolism, Xi'an Key Laboratory of Special Medicine and
Health
Engineering, Key Laboratory for Space Biosciences and Biotechnology, Research
Center
for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory
for
Bone Metabolism, School of Life Sciences, Northwestern Polytechnical
University,
Xi'an, China.
FAU - Patil, Suryaji
AU - Patil S
Health
Center
for
University,
Xi'an, China.
FAU - Gao, Yong-Guang
AU - Gao YG
Health
Center
for
University,
Xi'an, China.
FAU - Qian, Airong
AU - Qian A
Health
Center
for
University,
Xi'an, China.
LA - eng
PT - Review
DEP - 20200526
TA - Front Pharmacol
JT - Frontiers in pharmacology
JID - 101548923
PMC - PMC7264100
OTO - NOTNLM
OT - ECM
OT - bone cells
OT - bone formation
OT - bone repair
OT - bone tissue engineering
EDAT- 2020/06/13 06:00
MHDA- 2020/06/13 06:01
CRDT- 2020/06/13 06:00
PHST- 2019/12/19 00:00 [received]
PHST- 2020/05/06 00:00 [accepted]
PHST- 2020/06/13 06:00 [entrez]
PHST- 2020/06/13 06:00 [pubmed]
PHST- 2020/06/13 06:01 [medline]
AID - 10.3389/fphar.2020.00757 [doi]
PST - epublish
SO - Front Pharmacol. 2020 May 26;11:757. doi: 10.3389/fphar.2020.00757.
eCollection
2020.
PMID- 29472541
OWN - NLM
STAT- MEDLINE
DCOM- 20180501
LR - 20190222
IS - 2041-1723 (Linking)
VI - 9
IP - 1
DP - 2018 Feb 22
TI - Periosteum contains skeletal stem cells with high bone regenerative potential
controlled by Periostin.
PG - 773
LID - 10.1038/s41467-018-03124-z [doi]
LID - 773
AB - Bone regeneration relies on the activation of skeletal stem cells (SSCs) that
still
remain poorly characterized. Here, we show that periosteum contains SSCs with
high
bone regenerative potential compared to bone marrow stromal cells/skeletal
stem
cells (BMSCs) in mice. Although periosteal cells (PCs) and BMSCs are derived
from a
common embryonic mesenchymal lineage, postnatally PCs exhibit greater
clonogenicity,
growth and differentiation capacity than BMSCs. During bone repair, PCs can
efficiently contribute to cartilage and bone, and integrate long-term after
transplantation. Molecular profiling uncovers genes encoding Periostin and
other
extracellular matrix molecules associated with the enhanced response to
injury of
PCs. Periostin gene deletion impairs PC functions and fracture consolidation.
Periostin-deficient periosteum cannot reconstitute a pool of PCs after injury
demonstrating the presence of SSCs within periosteum and the requirement of

Periostin in maintaining this pool. Overall our results highlight the
importance of
analyzing periosteum and PCs to understand bone phenotypes.
FAU - Duchamp de Lageneste, Oriane
AU - Duchamp de Lageneste O
AD - INSERM UMR1163, Imagine Institute, Paris Descartes University, 75015, Paris,
France.
FAU - Julien, Anaïs
AU - Julien A
France.
FAU - Abou-Khalil, Rana
AU - Abou-Khalil R
France.
FAU - Frangi, Giulia
AU - Frangi G
France.
FAU - Carvalho, Caroline
AU - Carvalho C
France.
FAU - Cagnard, Nicolas
AU - Cagnard N
AD - Paris-Descartes Bioinformatics Platform, 75015, Paris, France.
FAU - Cordier, Corinne
AU - Cordier C
AD - INSERM US24 - CNRS UMS3633 Cytometry Platform, Paris Descartes University,
75015,
Paris, France.
FAU - Conway, Simon J
AU - Conway SJ
AD - Herman B. Wells Center for Pediatric Research, Department of Pediatrics,
Indiana
University School of Medicine, Indianapolis, IN, 46202, USA.
FAU - Colnot, Céline
AU - Colnot C
AUID- ORCID: 0000-0001-8423-8718
France.
celine.colnot@inserm.fr.
LA - eng
DEP - 20180222
TA - Nat Commun
JT - Nature communications
JID - 101528555
RN - 0 (Postn protein, mouse)
SB - IM
MH - Animals
MH - Cell Adhesion Molecules/genetics/*metabolism
MH - Female
MH - Male
MH - Mice
MH - Mice, Knockout
MH - Osteogenesis
MH - Periosteum/*cytology/metabolism
MH - Stem Cells/cytology/*metabolism
MH - Stromal Cells/cytology/metabolism
PMC - PMC5823889
COIS- The authors declare no competing financial interests.
EDAT- 2018/02/24 06:00
MHDA- 2018/05/02 06:00
CRDT- 2018/02/24 06:00
PHST- 2017/02/09 00:00 [received]
PHST- 2018/01/19 00:00 [accepted]
PHST- 2018/02/24 06:00 [entrez]
PHST- 2018/02/24 06:00 [pubmed]
PHST- 2018/05/02 06:00 [medline]
AID - 10.1038/s41467-018-03124-z [pii]
AID - 3124 [pii]
AID - 10.1038/s41467-018-03124-z [doi]
PST - epublish
SO - Nat Commun. 2018 Feb 22;9(1):773. doi: 10.1038/s41467-018-03124-z.
PMID- 30715998
OWN - NLM
STAT- MEDLINE
DCOM- 20190624
LR - 20190624
IS - 0361-7734 (Linking)
VI - 44
IP - 1
DP - 2019 Jan/Feb
TI - Fracture Resistance of Endodontically Treated Maxillary Premolars Restored
With
Different Methods.
PG - E1-E11
LID - 10.2341/17-262-L [doi]
AB - PURPOSE: The purpose of this in vitro study was to evaluate the resistance
and
patterns of fracture of endodontically treated maxillary premolars (ETPs)
restored
with different methods. METHODS AND MATERIALS: Mesio-occluso-distal cavities
were
prepared in 50 extracted caries-free human maxillary premolars after
endodontic
treatment. The teeth were divided into five groups (n=10), according to the
restorative method. G1: intact teeth (control group); G2: conventional
composite
resin; G3: conventional composite resin with a horizontal glass fiber post
inserted
between buccal and palatal walls; G4: bulk-fill flowable and bulk-fill
restorative
composites; and G5: ceramic inlay. For direct restorations, Filtek Z350 XT,
Filtek
Bulk Fill Flowable Restorative, and Filtek Bulk Fill Posterior Restorative
were
used. Indirect restorations were fabricated from a pressable lithium
disilicate
glass-ceramic (IPS e-max Press) and adhesively cemented (RelyX Ultimate). All
specimens were subjected to thermocycling (5°C to 55°C/5000 cycles) and

additionally
submitted to cyclic loading 50,000 times in an Electro-Mechanical Fatigue
Machine.
Next, the specimens were subjected to a compressive load at a crosshead speed
of 1
mm/min until fracture. The fractured specimens were analyzed to determine the
fracture pattern using a stereomicroscope, and then representative specimens

were
carbon coated to allow for the studying of the fracture surface under
scanning
electron microscopy. One-way analysis of variance (ANOVA) was used to compare
fracture resistance of the groups. The results of fracture patterns were

submitted
to the Fisher exact test (α=0.05). RESULTS: All specimens survived fatigue.
Mean
(standard deviation) failure loads (N) for groups were as follows: G1: 949.6
(331.5); G2: 999.6 (352.5); G3: 934.5 (233.6); G4: 771.0 (147.4); and G5:
856.7
(237.5). The lowest fracture resistance was recorded for G4, and the highest
ones
were recorded for G2, followed by that of G1 and G3. One-way ANOVA did not
reveal
significant differences between groups ( p>0.05). The highest repairable
fracture
rates were observed in G1 (100%) and G3 (80%). CONCLUSIONS: ETPs restored
with
conventional composite resin with or without horizontal fiber post, bulk-fill
composite, and ceramic inlay showed fracture resistance similar to that of

sound
teeth. Conventional composite resin restorations exhibited the highest
prevalence of
unrepairable fractures, and the insertion of a horizontal fiber post
decreased this
prevalence. Intact teeth showed 100% of repairable fractures. It is difficult
to
extrapolate the results directly to a clinical situation due to the
limitations of
this study.
FAU - Mergulhão, V A
AU - Mergulhão VA
FAU - de Mendonça, L S
AU - de Mendonça LS
FAU - de Albuquerque, M S
AU - de Albuquerque MS
FAU - Braz, R
AU - Braz R
LA - eng
PL - United States
TA - Oper Dent
JT - Operative dentistry
JID - 7605679
RN - 0 (Composite Resins)
RN - 0 (Filtek Bulk Fill)
RN - 0 (IPS e.max Press)
RN - 0 (Rely X Unicem)
RN - 0 (Resin Cements)
RN - 0 (fiberglass)
RN - 12001-21-7 (Dental Porcelain)
SB - D
MH - Bicuspid
MH - Composite Resins/chemistry
MH - Dental Porcelain
MH - Dental Restoration Failure
MH - Dental Restoration, Permanent/*methods
MH - Dental Stress Analysis
MH - Glass
MH - Humans
MH - In Vitro Techniques
MH - Inlays
MH - Maxilla
MH - Post and Core Technique
MH - Resin Cements
MH - Tooth Fractures/*prevention & control
MH - Tooth, Nonvital/*therapy
EDAT- 2019/02/05 06:00
MHDA- 2019/06/25 06:00
CRDT- 2019/02/05 06:00
PHST- 2019/02/05 06:00 [entrez]
PHST- 2019/02/05 06:00 [pubmed]
PHST- 2019/06/25 06:00 [medline]
AID - 10.2341/17-262-L [doi]
PST - ppublish
SO - Oper Dent. 2019 Jan/Feb;44(1):E1-E11. doi: 10.2341/17-262-L.
PMID- 31282388
OWN - NLM
STAT- MEDLINE
DCOM- 20200317
LR - 20200317
IS - 1748-6041 (Linking)
VI - 14
IP - 5
DP - 2019 Jul 19
TI - A tough and novel dual-response PAA/P(NiPAAM-co-PEGDMA) IPN hydrogels with
ceramics
by photopolymerization for consolidation of bone fragments following
fracture.
PG - 054101
LID - 10.1088/1748-605X/ab2fa3 [doi]
AB - In this work, a novel dual-response hydrogel for enhanced bone repair
following
multiple fractures was investigated. The conventional treatment of multiple
bone
fracture consists on removing smaller bone fragments from the body in a
surgery,
followed by the fixation of the bone using screws and plates. This work
proposes an
alternative for this treatment via in situ UV-initiated radical
polymerization of a
novel IPN hydrogel composed of PAA/P(NiPAAM-co-PEGDMA) incorporated with
ceramic
additives. The influence of different additives on mechanical properties and
sensitivity of the polymer, as well as the prepolymer mixture, were
investigated in
order to analyse the suitability of the composites for bone healing
applications.
This material exhibited an interpenetrating network, confirmed by FTIR, with
ceramics particles dispersed in between the polymer network. These structures
presented high strength by tensile tests, sensitivity to pH and temperature

and a
decrease on Tg values of NiPAAm depending on the amount of PEGDMA and
ceramics
added; although, the addition of ceramics to these composites did not
decrease their
stability drastically. Finally, cytotoxicity tests revealed variations on the
toxicity, whereas the addition of TCP presented to be non-toxic and that the
cell
viability increased when ceramics additives were incorporated into the
polymeric
matrix with an increased reporter activity of NF-κB, associated with aiding
fibroblast adhesion. Hence, it was possible to optimise feedstock ratios to
increase
the applicability of the prepolymer mixture as a potential treatment of
multiple
fractures.
FAU - de Lima, Gabriel Goetten
AU - de Lima GG
AD - Materials Research Institute, Athlone Institute of Technology, Athlone,
Ireland.
Universidade Federal do Paraná, Programa de Pós-Graduação em Engenharia e
Ciência
dos Materiais - PIPE, Curitiba, PR, Brazil.
FAU - Elter, Johanna Katrin
AU - Elter JK
FAU - Chee, Bor Shin
AU - Chee BS
FAU - Magalhães, Washington Luiz Esteves
AU - Magalhães WLE
FAU - Devine, Declan M
AU - Devine DM
FAU - Nugent, Michael J D
AU - Nugent MJD
FAU - de Sá, Marcelo J C
AU - de Sá MJC
LA - eng
DEP - 20190719
PL - England
TA - Biomed Mater
JT - Biomedical materials (Bristol, England)
JID - 101285195
RN - 0 (Acrylamides)
RN - 0 (Hydrogels)
RN - 0 (Methacrylates)
RN - 0 (NF-kappa B)
RN - 0 (Polymers)
RN - 0 (poly(ethylene glycol)-dimethacrylate)
RN - 3WJQ0SDW1A (Polyethylene Glycols)
RN - 91D9GV0Z28 (Durapatite)
SB - IM
MH - Acrylamides/*chemistry
MH - Animals
MH - Cell Adhesion
MH - Cell Survival
MH - Ceramics/chemistry
MH - Durapatite/chemistry
MH - Fibroblasts/metabolism
MH - Fracture Healing
MH - Fractures, Multiple/*therapy
MH - Glass
MH - Hydrogels/*chemistry
MH - Light
MH - Methacrylates/*chemistry
MH - Mice
MH - NF-kappa B/chemistry
MH - NIH 3T3 Cells
MH - Photochemistry
MH - Polyethylene Glycols/*chemistry
MH - Polymers/*chemistry/metabolism
MH - Spectroscopy, Fourier Transform Infrared
MH - Tensile Strength
EDAT- 2019/07/10 06:00
MHDA- 2020/03/18 06:00
CRDT- 2019/07/09 06:00
PHST- 2019/07/10 06:00 [pubmed]
PHST- 2020/03/18 06:00 [medline]
PHST- 2019/07/09 06:00 [entrez]
AID - 10.1088/1748-605X/ab2fa3 [doi]
PST - epublish
SO - Biomed Mater. 2019 Jul 19;14(5):054101. doi: 10.1088/1748-605X/ab2fa3.
PMID- 32752105
OWN - NLM
LR - 20200928
IS - 2079-4991 (Print)
IS - 2079-4991 (Linking)
VI - 10
IP - 8
DP - 2020 Jul 31
TI - Hydrogel as a Biomaterial for Bone Tissue Engineering: A Review.
LID - 10.3390/nano10081511 [doi]
LID - 1511
AB - Severe bone damage from diseases, including extensive trauma, fractures, and
bone
tumors, cannot self-heal, while traditional surgical treatment may bring side
effects such as infection, inflammation, and pain. As a new biomaterial with

controllable mechanical properties and biocompatibility, hydrogel is widely
used in
bone tissue engineering (BTE) as a scaffold for growth factor transport and
cell
adhesion. In order to make hydrogel more suitable for the local treatment of
bone
diseases, hydrogel preparation methods should be combined with synthetic
materials
with excellent properties and advanced technologies in different fields to
better
control drug release in time and orientation. It is necessary to establish a
complete method to evaluate the hydrogel's properties and biocompatibility
with the
human body. Moreover, establishment of standard animal models of bone defects
helps
in studying the therapeutic effect of hydrogels on bone repair, as well as to
evaluate the safety and suitability of hydrogels. Thus, this review aims to
systematically summarize current studies of hydrogels in BTE, including the
mechanisms for promoting bone synthesis, design, and preparation;
characterization
and evaluation methods; as well as to explore future applications of
hydrogels in
BTE.
FAU - Yue, Shuai
AU - Yue S
AD - College of Food Science and Technology, Huazhong Agricultural University,
Wuhan
430070, China.
AD - Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural
University, Ministry of Education, Wuhan 43000, China.
FAU - He, Hui
AU - He H
Wuhan
430070, China.
FAU - Li, Bin
AU - Li B
Wuhan
430070, China.
FAU - Hou, Tao
AU - Hou T
AUID- ORCID: 0000-0002-7567-2800
Wuhan
430070, China.
LA - eng
PT - Review
DEP - 20200731
TA - Nanomaterials (Basel)
JT - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC7466535
OTO - NOTNLM
OT - design
OT - evaluation methods
OT - hydrogels
OT - mechanism
COIS- The authors declare no conflict of interest.
EDAT- 2020/08/06 06:00
MHDA- 2020/08/06 06:01
CRDT- 2020/08/06 06:00
PHST- 2020/07/02 00:00 [received]
PHST- 2020/07/22 00:00 [revised]
PHST- 2020/07/29 00:00 [accepted]
PHST- 2020/08/06 06:00 [entrez]
PHST- 2020/08/06 06:00 [pubmed]
PHST- 2020/08/06 06:01 [medline]
AID - nano10081511 [pii]
AID - nanomaterials-10-01511 [pii]
AID - 10.3390/nano10081511 [doi]
PST - epublish
SO - Nanomaterials (Basel). 2020 Jul 31;10(8):1511. doi: 10.3390/nano10081511.
PMID- 32078704
OWN - NLM
STAT- MEDLINE
DCOM- 20210203
LR - 20210203
IS -
1863-9941 (Electronic)
IS -
1863-9933 (Print)
IS -
1863-9933 (Linking)
VI -
46
IP -
2
DP -
2020 Apr
TI -
Electrical stimulation in bone tissue engineering treatments.
PG -
231-244
LID -
10.1007/s00068-020-01324-1 [doi]
AB -
Electrical stimulation (EStim) has been shown to promote bone healing and
regeneration both in animal experiments and clinical treatments. Therefore,
incorporating EStim into promising new bone tissue engineering (BTE)
therapies is a
logical next step. The goal of current BTE research is to develop
combinations of
cells, scaffolds, and chemical and physical stimuli that optimize treatment
outcomes. Recent studies demonstrating EStim's positive osteogenic effects at
the
cellular and molecular level provide intriguing clues to the underlying
mechanisms
by which it promotes bone healing. In this review, we discuss results of
recent in
vitro and in vivo research focused on using EStim to promote bone healing and
regeneration and consider possible strategies for its application to improve

outcomes in BTE treatments. Technical aspects of exposing cells and tissues
to EStim
in in vitro and in vivo model systems are also discussed.
FAU - Leppik, Liudmila
AU - Leppik L
AUID- ORCID: 0000-0003-0362-4727
AD - Frankfurt Initiative for Regenerative Medicine, Experimental Orthopedics and
Trauma
Surgery, J.W. Goethe University, Frankfurt/Main, Germany.
Liudmila.Leppik@kgu.de.
FAU - Oliveira, Karla Mychellyne Costa
AU - Oliveira KMC
AUID- ORCID: 0000-0002-3338-793X
Trauma
FAU - Bhavsar, Mit Balvantray
AU - Bhavsar MB
AUID- ORCID: 0000-0002-7400-1263
Trauma
FAU - Barker, John Howard
AU - Barker JH
AUID- ORCID: 0000-0003-0816-3413
Trauma
LA - eng
PT - Review
DEP - 20200220
TA - Eur J Trauma Emerg Surg
JT - European journal of trauma and emergency surgery : official publication of
the
European Trauma Society
JID - 101313350
RN - 0 (Heat-Shock Proteins)
RN - 0 (Reactive Oxygen Species)
RN - 0 (Receptors, Cell Surface)
RN - 8L70Q75FXE (Adenosine Triphosphate)
SB - IM
MH - Adenosine Triphosphate/metabolism
MH - Apoptosis
MH - *Bone and Bones
MH - Calcium Signaling
MH - Cell Adhesion
MH - Cell Differentiation
MH - Cell Movement
MH - Chondrogenesis
MH - Dental Pulp/cytology
MH - Electric Stimulation/*methods
MH - Electric Stimulation Therapy/*methods
MH - Guided Tissue Regeneration/*methods
MH - Heat-Shock Proteins/metabolism
MH - Humans
MH - In Vitro Techniques
MH - Inflammation
MH - MAP Kinase Signaling System
MH - Mechanotransduction, Cellular
MH - Membrane Microdomains
MH - Mesenchymal Stem Cells
MH - Neovascularization, Physiologic
MH - Osteoblasts
MH - Osteogenesis
MH - Reactive Oxygen Species/metabolism
MH - Receptors, Cell Surface/metabolism
MH - Signal Transduction
MH - Tissue Engineering/*methods
PMC - PMC7113220
OTO - NOTNLM
OT - Bone regeneration
OT - Bone tissue engineering
OT - Electrical stimulation
OT - In vitro
OT - In vivo
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/02/23 06:00
MHDA- 2021/02/04 06:00
CRDT- 2020/02/21 06:00
PHST- 2019/12/10 00:00 [received]
PHST- 2020/02/04 00:00 [accepted]
PHST- 2020/02/23 06:00 [pubmed]
PHST- 2021/02/04 06:00 [medline]
PHST- 2020/02/21 06:00 [entrez]
AID - 10.1007/s00068-020-01324-1 [pii]
AID - 1324 [pii]
AID - 10.1007/s00068-020-01324-1 [doi]
PST - ppublish
SO - Eur J Trauma Emerg Surg. 2020 Apr;46(2):231-244. doi: 10.1007/s00068-020-
01324-1.
Epub 2020 Feb 20.
PMID- 31629040
OWN - NLM
STAT- MEDLINE
DCOM- 20201008
LR - 20201008
IS - 0168-3659 (Linking)
VI - 313
DP - 2019 Nov 10
TI - Nanogels for regenerative medicine.
PG - 148-160
LID - S0168-3659(19)30555-3 [pii]
LID - 10.1016/j.jconrel.2019.09.015 [doi]
AB - Nanogels have been widely explored for drug delivery, but their applications
in the
tissue engineering field are still quite recent. Regenerative medicine also
demands
controlled delivery of growth factors and other active substances able to
promote
cell adhesion and guide cell differentiation and tissue formation. Moreover,
nanogels could be added to tissue scaffolds for modifying their inner
architecture,
texture and mechanical properties, which are critical for regulating cell
behavior.
This review aims to provide an insight into the different roles that nanogels
may
play for improving tissue regeneration. Last decade literature has been
carefully
analyzed with a focus on in vivo outcomes. After an introductory section to
nanogels, relevant examples of their performance for skin and bone tissue
regeneration applications are discussed. Healing of chronic wounds and
critical size
bone fractures may significantly improve thanks to the use of nanogels solely
or in
combination with scaffolds. Nanogel roles in regenerating vessels, cardiac
tissue,
urothelium and urethral muscle tissue are also presented. Overall, the
information
gathered in the review clearly highlights the relevance of multidisciplinary
approaches to design nanogels that can face up to the needs of the
regenerative
medicine. Nanogels may help bring together researchers working in active
ingredient
formulation, controlled release, nanomechanics, tissue engineering and
scaffolding
with the common purpose of developing clinically relevant tools for the
complete
regeneration of complex tissues.
FAU - Grimaudo, Maria Aurora
AU - Grimaudo MA
AD - Departamento de Farmacología, Fa+rmacia y Tecnología Farmacéutica, I+D Farma
Group
(GI-1645), Facultad de Farmacia and Health Research Institute of Santiago de
Compostela (IDIS), Universidade de Santiago de Compostela, 15782-Santiago de
Compostela, Spain; Nano-Oncology Unit, Translational Medical Oncology Group,
Health
Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela,
Spain.
Electronic address: Maria.Aurora.Grimaudo@sergas.es.
FAU - Concheiro, Angel
AU - Concheiro A
Group
Compostela, Spain.
FAU - Alvarez-Lorenzo, Carmen
AU - Alvarez-Lorenzo C
Group
Compostela, Spain. Electronic address: carmen.alvarez.lorenzo@usc.es.
LA - eng
PT - Review
DEP - 20191016
PL - Netherlands
TA - J Control Release
JT - Journal of controlled release : official journal of the Controlled Release
Society
JID - 8607908
RN - 0 (Cross-Linking Reagents)
RN - 0 (Drug Carriers)
RN - 0 (Nanogels)
RN - 0 (Polymers)
SB - IM
MH - Animals
MH - Biocompatible Materials/*chemistry
MH - Biophysical Phenomena
MH - Blood Vessels/physiology
MH - Bone and Bones/physiology
MH - Cross-Linking Reagents/chemistry
MH - Drug Carriers/chemistry
MH - Drug Liberation
MH - Heart/physiology
MH - Humans
MH - Muscles/physiology
MH - Nanogels/*chemistry
MH - Polymers/chemistry
MH - *Regeneration
MH - Regenerative Medicine
MH - Skin/metabolism
MH - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT - *Bone tissue engineering
OT - *Cardiac repair
OT - *Controlled release
OT - *Nanogel
OT - *Regenerative medicine
OT - *Wound healing
EDAT- 2019/10/20 06:00
MHDA- 2020/10/09 06:00
CRDT- 2019/10/20 06:00
PHST- 2019/07/30 00:00 [received]
PHST- 2019/09/19 00:00 [revised]
PHST- 2019/09/23 00:00 [accepted]
PHST- 2019/10/20 06:00 [pubmed]
PHST- 2020/10/09 06:00 [medline]
PHST- 2019/10/20 06:00 [entrez]
AID - S0168-3659(19)30555-3 [pii]
AID - 10.1016/j.jconrel.2019.09.015 [doi]
PST - ppublish
SO - J Control Release. 2019 Nov 10;313:148-160. doi:
10.1016/j.jconrel.2019.09.015. Epub
2019 Oct 16.
PMID- 32706234
OWN - NLM
STAT- MEDLINE
DCOM- 20210222
LR - 20210222
IS - 1944-8244 (Linking)
VI - 12
IP - 33
DP - 2020 Aug 19
TI - Nanoscaled Bionic Periosteum Orchestrating the Osteogenic Microenvironment
for
Sequential Bone Regeneration.
PG - 36823-36836
LID - 10.1021/acsami.0c06906 [doi]
AB - Periosteum orchestrates bone repair. Previously developed artificial
periosteum was
mainly focusing on materials modification to simply enhance bone formation,
but few
were attempting to make the artificial periosteum fit different bone repair
stages.
Here, we constructed a functionalized periosteum, which was composed of an
electrospun scaffold grafted with leptin receptor antibody (LepR-a) and BMP2-
loaded
hollow MnO(2) (h-MnO(2)) nanoparticles through a polydopamine (PDA)-assisted
technique. The bionic periosteum showed suitable mechanical properties and
favorable
biocompatibility. It effectively recruited skeletal stem cells (SSCs) through
antigen-antibody interactions, as in in vitro cell adhesion tests, we

observed that
more SSCs attached to the LepR-a-grafted periosteum compared to the control
group.
In vivo, the LepR-a-grafted periosteum covered on the cranial defect in
Prx1-Cre/ERT2, -EGFP mice recruited more Prx1-EGFP cells to the fracture site
compared to control groups at post-surgery day 3, 7, and 14. Co-staining with

Sp7
indicated that most of the recruited Prx1-EGFP cells underwent osteogenic
lineage
commitment. Sustained BMP2 release from h-MnO(2) promoted osteogenesis by
accelerating the osteogenic differentiation of recruited SSCs, as
demonstrated by
alkaline phosphatase (ALP) and alizarin red staining (ARS) in vitro and
microcomputed tomography (micro-CT) in vivo. Interestingly, we also observed
the
growth of osteogenic coupled capillaries (CD31(hi)Emcn(hi)) in the bone
repair site,
which might be induced by increased platelet-derived growth factor-BB (PDGF-
BB) in
the regenerative microenvironment subsequent to SSCs' differentiation. Taken
together, the findings from this study indicate that the multifunctionalized
periosteum efficiently recruited and motivated the SSCs in vivo and
orchestrated the
osteogenic microenvironment for bone repair in a sequence manner. Thus, the
construction of the bionic periosteum to couple with natural bone
regeneration
stages has been demonstrated to be effective in facilitating bone healing.
FAU - Li, Hanwen
AU - Li H
AD - Department of Orthopedic Surgery, The First Affiliated Hospital of Soochow
University, 899 Pinghai Road, Suzhou, Jiangsu 215000, P. R. China.
FAU - Wang, Huan
AU - Wang H
AD - Orthopedic Institute, Medical College, Soochow University, 708 Renmin Road,
Suzhou,
Jiangsu 215000, P. R. China.
FAU - Pan, Jun
AU - Pan J
FAU - Li, Jiaying
AU - Li J
Suzhou,
FAU - Zhang, Kai
AU - Zhang K
FAU - Duan, Weifeng
AU - Duan W
FAU - Liang, Huan
AU - Liang H
AD - Medical College, Yangzhou University, 136 Jiangyang Road, Yangzhou, Jiangsu
225009,
P. R. China.
FAU - Chen, Kangwu
AU - Chen K
FAU - Geng, Dechun
AU - Geng D
FAU - Shi, Qin
AU - Shi Q
Suzhou,
FAU - Yang, Huilin
AU - Yang H
Suzhou,
FAU - Li, Bin
AU - Li B
AUID- ORCID: 0000-0001-8516-9953
Suzhou,
FAU - Chen, Hao
AU - Chen H
AUID- ORCID: 0000-0002-4864-5030
AD - Medical College, Yangzhou University, 136 Jiangyang Road, Yangzhou, Jiangsu
225009,
P. R. China.
LA - eng
DEP - 20200807
PL - United States
TA - ACS Appl Mater Interfaces
JT - ACS applied materials & interfaces
JID - 101504991
RN - 0 (Homeodomain Proteins)
RN - 0 (Indoles)
RN - 0 (Manganese Compounds)
RN - 0 (Oxides)
RN - 0 (Polymers)
RN - 0 (Receptors, Leptin)
RN - 0 (polydopamine)
RN - 094ZI81Y45 (Tamoxifen)
RN - EC 3.1.3.1 (Alkaline Phosphatase)
RN - TF219GU161 (manganese dioxide)
SB - IM
MH - Alkaline Phosphatase/metabolism
MH - Animals
MH - Bone Morphogenetic Protein 2/metabolism
MH - Homeodomain Proteins/metabolism
MH - Humans
MH - Indoles/*chemistry
MH - Male
MH - Manganese Compounds/*chemistry
MH - Mice
MH - Models, Animal
MH - Mouse Embryonic Stem Cells
MH - Nanostructures/*chemistry
MH - Osteogenesis
MH - Oxides/*chemistry
MH - Periosteum/*metabolism
MH - Polymers/*chemistry
MH - Rats
MH - Receptors, Leptin/metabolism
MH - Tamoxifen/metabolism
OTO - NOTNLM
OT - bionic periosteum
OT - electrospun
OT - hollow MnO2 nanoparticles
OT - leptin receptor antibody
OT - osteogenic microenvironment
EDAT- 2020/07/25 06:00
MHDA- 2021/02/23 06:00
CRDT- 2020/07/25 06:00
PHST- 2020/07/25 06:00 [pubmed]
PHST- 2021/02/23 06:00 [medline]
PHST- 2020/07/25 06:00 [entrez]
AID - 10.1021/acsami.0c06906 [doi]
PST - ppublish
SO - ACS Appl Mater Interfaces. 2020 Aug 19;12(33):36823-36836. doi:
10.1021/acsami.0c06906. Epub 2020 Aug 7.
PMID- 28631442
OWN - NLM
STAT- MEDLINE
DCOM- 20190315
LR - 20190315
IS - 1464-7931 (Print)
IS - 0006-3231 (Linking)
VI - 93
IP - 1
DP - 2018 Feb
TI - Osteoblast migration in vertebrate bone.
PG - 350-363
LID - 10.1111/brv.12345 [doi]
AB - Bone formation, for example during bone remodelling or fracture repair,
requires
mature osteoblasts to deposit bone with remarkable spatial precision. As
osteoblast
precursors derive either from circulation or resident stem cell pools, they
and
their progeny are required to migrate within the three-dimensional bone space
and to
navigate to their destination, i.e. to the site of bone formation. An
understanding
of this process is emerging based on in vitro and in vivo studies of several
vertebrate species. Receptors on the osteoblast surface mediate cell adhesion
and
polarization, which induces osteoblast migration. Osteoblast migration is
then
facilitated along gradients of chemoattractants. The latter are secreted or
released
proteolytically by several cell types interacting with osteoblasts, including
osteoclasts and vascular endothelial cells. The positions of these cellular

sources
of chemoattractants in relation to the position of the osteoblasts provide
the
migrating osteoblasts with tracks to their destination, and osteoblasts
possess the
means to follow a track marked by multiple chemoattractant gradients. In
addition to
chemotactic cues, osteoblasts sense other classes of signals and utilize them
as
landmarks for navigation. The composition of the osseous surface guides
adhesion and
hence migration efficiency and can also provide steering through haptotaxis.
Further, it is likely that signals received from surface interactions
modulate
chemotaxis. Besides the nature of the surface, mechanical signals such as
fluid flow
may also serve as navigation signals for osteoblasts. Alterations in
osteoblast
migration and navigation might play a role in metabolic bone diseases such as
osteoporosis.
CI - © 2017 Cambridge Philosophical Society.
FAU - Thiel, Antonia
AU - Thiel A
AD - Bone Cell and Imaging Laboratory, Department of Orthopedics, Klinikum rechts
der
Isar, Ismaninger Straße 22, Technical University Munich, 81675 München,
Germany.
FAU - Reumann, Marie K
AU - Reumann MK
AD - Siegfried Weller Institute, BG Hospital, University of Tübingen,
Schnarrenbergstraße
95, 72076 Tübingen, Germany.
FAU - Boskey, Adele
AU - Boskey A
AD - Mineralized Tissue Laboratory, Research Division, Hospital for Special
Surgery, 535
E 70th Street, New York, NY 10021, U.S.A.
FAU - Wischmann, Johannes
AU - Wischmann J
der
Germany.
FAU - von Eisenhart-Rothe, Rüdiger
AU - von Eisenhart-Rothe R
der
Germany.
FAU - Mayer-Kuckuk, Philipp
AU - Mayer-Kuckuk P
der
Germany.
LA - eng
PT - Review
DEP - 20170619
TA - Biol Rev Camb Philos Soc
JT - Biological reviews of the Cambridge Philosophical Society
JID - 0414576
SB - IM
MH - Animals
MH - Bone and Bones/*cytology/*physiology
MH - Osteoblasts/*physiology
MH - Vertebrates/*physiology
PMC - PMC6218945
MID - NIHMS884047
OTO - NOTNLM
OT - *bone
OT - *cell migration
OT - *chemotaxis
OT - *fluid flow
OT - *mineralized surfaces
OT - *osteoblasts
EDAT- 2017/06/21 06:00
MHDA- 2019/03/16 06:00
CRDT- 2017/06/21 06:00
PHST- 2016/08/08 00:00 [received]
PHST- 2017/05/09 00:00 [revised]
PHST- 2017/05/12 00:00 [accepted]
PHST- 2017/06/21 06:00 [pubmed]
PHST- 2019/03/16 06:00 [medline]
PHST- 2017/06/21 06:00 [entrez]
AID - 10.1111/brv.12345 [doi]
PST - ppublish
SO - Biol Rev Camb Philos Soc. 2018 Feb;93(1):350-363. doi: 10.1111/brv.12345.
Epub 2017
Jun 19.
PMID- 28929186
OWN - NLM
STAT- MEDLINE
DCOM- 20190115
LR - 20190115
VI - 57
IP - 11
DP - 2017 Nov
TI - [When is cartilage repair successful?].
PG - 907-914
LID - 10.1007/s00117-017-0305-0 [doi]
AB - Focal cartilage lesions are a cause of long-term disability and morbidity.
After
cartilage repair, it is crucial to evaluate long-term progression or failure
in
a reproducible, standardized manner. This article provides an overview of the
different cartilage repair procedures and important characteristics to look
for in
cartilage repair imaging. Specifics and pitfalls are pointed out alongside
general
aspects. After successful cartilage repair, a complete, but not hypertrophic
filling
of the defect is the primary criterion of treatment success. The repair
tissue
should also be completely integrated to the surrounding native cartilage.
After some
months, the transplants signal should be isointense compared to native
cartilage.
Complications like osteophytes, subchondral defects, cysts, adhesion and
chronic
bone marrow edema or joint effusion are common and have to be observed via
follow-up. Radiological evaluation and interpretation of postoperative
changes
should always take the repair method into account.
FAU - Raudner, M
AU - Raudner M
AD - Exzellenzzentrum Hochfeld-MR, Universitätsklinik für Radiologie und
Nuklearmedizin,
Medizinische Universität Wien, Lazarettgasse 14, 1090, Wien, Österreich.
FAU - Schreiner, M M
AU - Schreiner MM
AD - Universitätsklinik für Orthopädie, Medizinische Universität Wien,
Währinger-Gürtel 18-20, 1090, Wien, Österreich.
FAU - Röhrich, S
AU - Röhrich S
Nuklearmedizin,
FAU - Zalaudek, M
AU - Zalaudek M
Nuklearmedizin,
FAU - Trattnig, S
AU - Trattnig S
Nuklearmedizin,
siegfried.trattnig@meduniwien.ac.at.
LA - ger
PT - Review
TT - Wann ist eine Knorpelreparatur erfolgreich?
PL - Germany
TA - Radiologe
JT - Der Radiologe
JID - 0401257
SB - IM
MH - Cartilage, Articular/diagnostic imaging/*injuries/physiopathology/*surgery
MH - Fractures, Cartilage/diagnostic imaging/physiopathology/*surgery
MH - Humans
MH - *Magnetic Resonance Imaging
MH - Postoperative Complications/*diagnostic imaging/physiopathology/surgery
OTO - NOTNLM
OT - Aftercare
OT - Cartilage defect
OT - Cartilage repair
OT - Magnetic resonance tomography
OT - Osteoarthritis
EDAT- 2017/09/21 06:00
MHDA- 2019/01/16 06:00
CRDT- 2017/09/21 06:00
PHST- 2017/09/21 06:00 [pubmed]
PHST- 2019/01/16 06:00 [medline]
PHST- 2017/09/21 06:00 [entrez]
AID - 10.1007/s00117-017-0305-0 [pii]
AID - 10.1007/s00117-017-0305-0 [doi]
PST - ppublish
SO - Radiologe. 2017 Nov;57(11):907-914. doi: 10.1007/s00117-017-0305-0.
PMID- 27416302
OWN - NLM
STAT- MEDLINE
DCOM- 20181220
LR - 20181220
VI - 27
IP - 5
DP - 2018 Jun
TI - Ratios of Cantilever Lengths and Anterior-Posterior Spreads of Definitive
Hybrid
Full-Arch, Screw-Retained Prostheses: Results of a Clinical Study.
PG - 402-408
LID - 10.1111/jopr.12519 [doi]
AB - PURPOSE: To record the distal cantilever lengths (CL) of full-arch,
definitive
hybrid prostheses fabricated for patients after treatment with an immediate
occlusal
loading protocol. Anterior/posterior (AP) spreads were measured on master
casts of
the definitive prostheses. CL/AP ratios were calculated for these 2
parameters.
These measurements were then compared and evaluated for statistical and
clinical
significance; the CL/AP ratios were also compared between definitive and
interim
prostheses. MATERIALS AND METHODS: One hundred thirty patients with 193
edentulous
arches (112 maxillary; 81 mandibular; 191 arches restored with 4 implants; 2
maxillary arches restored with 5 implants) were treated. Seven hundred
seventy-four
implants (Nobel Biocare Brånemark System [Nobel Active]) were included in
this
report. All but 2 patients had 4 implants placed into each jaw: the anterior
implants were relatively vertical; the posterior implants were tilted
parallel to
the anterior wall of the maxillary sinus and angled distally above the mental
foramen. Patients were treated and followed in private practice by the

author.
Implants had to have at least 35 Ncm of insertion torque to be immediately
loaded.
All implants were immediately loaded with full functional occlusions via
interim,
full-arch, all-acrylic resin prostheses. Definitive full-arch, hybrid
prostheses
were fabricated approximately 6 to 9 months after implant placement with
computer-aided design/computer-aided manufacturing (CAD/CAM) frameworks,
denture
bases, and acrylic resin denture teeth. Measurements of the distal
cantilevered
segments were made with a Boley gauge on the interim and definitive
prostheses prior
to insertion. AP spreads were measured on the master casts made from abutment
level
impressions approximately 4 months post-occlusal loading. Prosthetic
complications
such as denture base fractures and cohesive/adhesive denture tooth fractures
were
recorded in the charts as they occurred. All charts were reviewed for this
report.
Prosthetic repairs for the definitive prostheses were analyzed by type (tooth
or
denture base), arch, gender, and location within the edentulous arches.
RESULTS:
Patients were followed for up to 48 months post-immediate occlusal loading.
One
patient experienced maxillary implant failure; the overall implant survival
rate
(SR) was 99.5% (770 of 774). Four hundred forty-six of 450 maxillary implants
and
324 of 324 mandibular implants survived for SRs of 99.1% and 100%,
respectively.
Thirty-three of the 193 interim prostheses (17.1%) warranted at least one
repair
during treatment. One of the 193 definitive prostheses demonstrated a
posterior
denture base fracture. The average cantilevered segments for the definitive
maxillary prostheses were 15.6 mm (right) and 15.4 mm (left). The average
cantilevered segments for the definitive mandibular prostheses were 15.5 mm
(right)
and 15.6 mm (left). The average maxillary AP spread was 18.4 mm; the average
mandibular AP spread was 17.3 mm. Average maxillary CL/AP spread ratios were
0.85
(right) and 0.84 (left); average mandibular CL/AP spread ratios were 0.89
(right)
and 0.90 (left). There were no statistically significant associations between
the
CL/AP ratios and the frequency or type of prosthetic repairs recorded in this
study.
CONCLUSIONS: The results from this 4-year clinical retrospective analysis
indicated
that one of 130 patients experienced implant failures. The prosthetic
complication
rate for the definitive prostheses in this study was less than 1% (0.005).
The
author suggests that the parameters used in this study's framework designs
for
full-arch, titanium milled frameworks (CL/AP ratio <1), resulted in
consistent,
predictable results for rehabilitating edentulous patients.
CI - © 2016 by the American College of Prosthodontists.
FAU - Drago, Carl
AU - Drago C
AD - Gundersen Health System, LaCrosse, WI.
AD - Marquette University School of Dentistry, Milwaukee, WI.
LA - eng
PT - Clinical Study
DEP - 20160714
PL - United States
TA - J Prosthodont
JT - Journal of prosthodontics : official journal of the American College of
Prosthodontists
JID - 9301275
SB - D
MH - Bone Screws
MH - Computer-Aided Design
MH - Dental Prosthesis Design
MH - *Dental Prosthesis, Implant-Supported
MH - Denture Design
MH - *Denture, Complete
MH - Female
MH - Humans
MH - Immediate Dental Implant Loading/*methods
MH - Jaw, Edentulous/*rehabilitation
MH - Male
MH - Retrospective Studies
MH - Treatment Outcome
OTO - NOTNLM
OT - Dental implants
OT - immediate full-arch loading
OT - interim/definitive prostheses
OT - osseointegration
EDAT- 2016/07/15 06:00
MHDA- 2018/12/21 06:00
CRDT- 2016/07/15 06:00
PHST- 2016/05/30 00:00 [accepted]
PHST- 2016/07/15 06:00 [pubmed]
PHST- 2018/12/21 06:00 [medline]
PHST- 2016/07/15 06:00 [entrez]
AID - 10.1111/jopr.12519 [doi]
PST - ppublish
SO - J Prosthodont. 2018 Jun;27(5):402-408. doi: 10.1111/jopr.12519. Epub 2016 Jul
14.
PMID- 29662799
OWN - NLM
LR - 20201001
IS - 2214-031X (Print)
VI - 9
DP - 2017 Apr
TI - Ultrasound as a stimulus for musculoskeletal disorders.
PG - 52-59
LID - 10.1016/j.jot.2017.03.004 [doi]
AB - Ultrasound is an inaudible form of acoustic sound wave at 20 kHz or above
that is
widely used in the medical field with applications including medical imaging
and
therapeutic stimulation. In therapeutic ultrasound, low-intensity pulsed
ultrasound
(LIPUS) is the most widely used and studied form that generally uses acoustic
waves
at an intensity of 30 mW/cm(2), with 200 ms pulses and 1.5 MHz. In
orthopaedic
applications, it is used as a biophysical stimulus for musculoskeletal tissue
repair
to enhance tissue regeneration. LIPUS has been shown to enhance fracture
healing by
shortening the time to heal and reestablishment of mechanical properties
through
enhancing different phases of the healing process, including the inflammatory
phase,
callus formation, and callus remodelling phase. Reports from in vitro studies
reveal
insights in the mechanism through which acoustic stimulations activate cell
surface
integrins that, in turn, activate various mechanical transduction pathways
including
FAK (focal adhesion kinase), ERK (extracellular signal-regulated kinase),
PI3K, and
Akt. It is then followed by the production of cyclooxygenase 2 and
prostaglandin E2
to stimulate further downstream angiogenic, osteogenic, and chondrogenic
cytokines,
explaining the different enhancements observed in animal and clinical
studies.
Furthermore, LIPUS has also been shown to have remarkable effects on
mesenchymal
stem cells (MSCs) in musculoskeletal injuries and tissue regeneration. The
recruitment of MSCs to injury sites by LIPUS requires the SDF-1 (stromal cell
derived factor-1)/CXCR-4 signalling axis. MSCs would then differentiate

differently,
and this is regulated by the presence of different cytokines, which
determines their
fates. Other musculoskeletal applications including bone-tendon junction
healing,
and distraction osteogenesis are also explored, and the results are
promising.
However, the use of LIPUS is controversial in treating osteoporosis, with
negative
findings in clinical settings, which may be attributable to the absence of an
injury
entry point for the acoustic signal to propagate, strong attenuation effect
of
cortical bone and the insufficient intensity for penetration, whereas in some
animal
studies it has proven effective.
FAU - Zhang, Ning
AU - Zhang N
AD - Musculoskeletal Research Laboratory, Department of Orthopaedics and
Traumatology,
The Chinese University of Hong Kong, Hong Kong Special Administrative Region,
China.
FAU - Chow, Simon Kwoon-Ho
AU - Chow SK
Traumatology,
China.
AD - The Chinese University of Hong Kong - Astronaut Center of China (CUHK-ACC)
Space
Medicine Centre on Health Maintenance of Musculoskeletal System, The Chinese
University of Hong Kong Shenzhen Research Institute, Shenzhen, China.
FAU - Leung, Kwok-Sui
AU - Leung KS
Traumatology,
China.
FAU - Cheung, Wing-Hoi
AU - Cheung WH
Traumatology,
China.
AD - The Chinese University of Hong Kong - Astronaut Center of China (CUHK-ACC)
Space
Medicine Centre on Health Maintenance of Musculoskeletal System, The Chinese
University of Hong Kong Shenzhen Research Institute, Shenzhen, China.
LA - eng
PT - Review
DEP - 20170405
TA - J Orthop Translat
JT - Journal of orthopaedic translation
JID - 101625127
PMC - PMC5822964
OTO - NOTNLM
OT - LIPUS
OT - fracture healing
OT - musculoskeletal repair
OT - stem cell differentiation
OT - stem cell recruitment
EDAT- 2018/04/18 06:00
MHDA- 2018/04/18 06:01
CRDT- 2018/04/18 06:00
PHST- 2018/04/18 06:00 [entrez]
PHST- 2018/04/18 06:00 [pubmed]
PHST- 2018/04/18 06:01 [medline]
AID - S2214-031X(16)30270-4 [pii]
AID - 10.1016/j.jot.2017.03.004 [doi]
PST - epublish
SO - J Orthop Translat. 2017 Apr 5;9:52-59. doi: 10.1016/j.jot.2017.03.004.
eCollection
2017 Apr.
PMID- 32903290
OWN - NLM
LR - 20210610
IS - 2524-4426 (Linking)
VI - 1
DP - 2019
TI - A biomechanical test model for evaluating osseous and osteochondral tissue
adhesives.
PG - 11
LID - 10.1186/s42490-019-0011-2 [doi]
LID - 11
AB - BACKGROUND: Currently there are no standard models with which to evaluate the
biomechanical performance of calcified tissue adhesives, in vivo. We present,
herein, a pre-clinical murine distal femoral bone model for evaluating tissue
adhesives intended for use in both osseous and osteochondral tissue

reconstruction.
RESULTS: Cylindrical cores (diameter (Ø) 2 mm (mm) × 2 mm depth), containing
both
cancellous and cortical bone, were fractured out from the distal femur and
then
reattached using one of two tissue adhesives. The adhesiveness of fibrin glue
(Tisseel(tm)), and a novel, biocompatible, calcium phosphate-based tissue

adhesive
(OsStic(tm)) were evaluated by pullout testing, in which glued cores were
extracted
and the peak force at failure recorded. The results show that Tisseel weakly
bonded
the metaphyseal bone cores, while OsStic produced > 30-fold higher mean peak
forces
at failure (7.64 Newtons (N) vs. 0.21 N). The failure modes were consistently
disparate, with Tisseel failing gradually, while OsStic failed abruptly, as

would be
expected with a calcium-based material. Imaging of the bone/adhesive
interface with
microcomputed tomography revealed that, for OsStic, failure occurred more
often
within cancellous bone (75% of tested samples) rather than at the adhesive
interface. CONCLUSIONS: Despite the challenges associated with biomechanical
testing
in small rodent models the preclinical ex-vivo test model presented herein is
both
sensitive and accurate. It enabled differences in tissue adhesive strength to
be
quantified even for very small osseous fragments (<Ø4mm). Importantly, this
model
can easily be scaled to larger animals and adapted to fracture fragment
fixation in
human bone. The present model is also compatible with other long-term in vivo
evaluation methods (i.e. in vivo imaging, histological analysis, etc.).

CI - © The Author(s) 2019.
FAU - Procter, Philip
AU - Procter P
AUID- ORCID: 0000-0002-3219-1177
AD - Division of Applied Materials Science, Department of Engineering Sciences,
Uppsala
University, Box 523, 75120 Uppsala, Sweden. ISNI: 0000 0004 1936 9457. GRID:
grid.8993.b
AD - GPBio Ltd, Rathkeale, Ireland.
FAU - Pujari-Palmer, Michael
AU - Pujari-Palmer M
Uppsala
grid.8993.b
FAU - Hulsart-Billström, Gry
AU - Hulsart-Billström G
Uppsala
grid.8993.b
AD - Department Surgical Sciences, Orthopaedics, Uppsala University Hospital,
75185
Uppsala, Sweden. ISNI: 0000 0001 2351 3333. GRID: grid.412354.5
FAU - Wenner, David
AU - Wenner D
Uppsala
grid.8993.b
FAU - Insley, Gerard
AU - Insley G
Uppsala
grid.8993.b
AD - GPBio Ltd, Rathkeale, Ireland.
FAU - Larsson, Sune
AU - Larsson S
AD - Department Surgical Sciences, Orthopaedics, Uppsala University Hospital,
75185
Uppsala, Sweden. ISNI: 0000 0001 2351 3333. GRID: grid.412354.5
FAU - Engqvist, Håkan
AU - Engqvist H
Uppsala
grid.8993.b
LA - eng
GR - T32 HS000046/HS/AHRQ HHS/United States
DEP - 20190507
TA - BMC Biomed Eng
JT - BMC biomedical engineering
JID - 101756092
PMC - PMC7422571
OTO - NOTNLM
OT - Biomechanical model
OT - Bone adhesive
OT - Calcium phosphate cements
OT - Fracture repair
OT - Orthobiologics
OT - Phosphoserine
OT - Tissue adhesive
COIS- Competing interestsThe following authors declare partial ownership in a
company that
owns all related intellectual property (GPBio LTD): Michael Pujari-Palmer
(M.P.),
Gerard Insley (G.I.), Philip Procter (P.P.), Håkan Engqvist (H.E.).
EDAT- 2019/05/07 00:00
MHDA- 2019/05/07 00:01
CRDT- 2020/09/09 17:53
PHST- 2019/01/04 00:00 [received]
PHST- 2019/03/31 00:00 [accepted]
PHST- 2020/09/09 17:53 [entrez]
PHST- 2019/05/07 00:00 [pubmed]
PHST- 2019/05/07 00:01 [medline]
AID - 11 [pii]
AID - 10.1186/s42490-019-0011-2 [doi]
PST - epublish
SO - BMC Biomed Eng. 2019 May 7;1:11. doi: 10.1186/s42490-019-0011-2. eCollection
2019.
PMID- 31904174
OWN - NLM
STAT- In-Process
LR - 20201125
IS - 1549-3296 (Print)
IS - 1549-3296 (Linking)
VI - 108
IP - 4
DP - 2020 Apr
TI - Self-assembled biomimetic Nano-Matrix for stem cell anchorage.
PG - 984-991
LID - 10.1002/jbm.a.36875 [doi]
AB - Mesenchymal stem cells (MSCs) have been widely applied in biomedicine due to
their
ability to differentiate into many different cell types and their ability to
synthesize a broad spectrum of growth factors and cytokines that directly and
indirectly influence other cells in their vicinity. To guide MSC infiltration

to a
bone fracture site, we developed a novel self-assembled Nano-Matrix which can
be
used as an injectable scaffold to repair bone fractures. The Nano-Matrix is
formed
by Janus base nanotubes (JBNTs) and fibronectin (FN). JBNTs are nucleobase-
derived
nanotubes mimicking collagen fibers, and FN is one of the cell adhesive
glycoproteins which is responsible for cell-extracellular matrix interactions
and
guides stem cell migration and differentiation to desired cells types. Here,
we
demonstrated the successful fabrication and characterization of the JBNT/FN
Nano-Matrix as well as its excellent bioactivity that encouraged human MSC
migration
and adhesion. This work lays a solid foundation for using the Nano-Matrix as
an
injectable approach to improve MSC retention and function during bone
fracture
healing.
CI - © 2020 Wiley Periodicals, Inc.
FAU - Zhou, Libo
AU - Zhou L
AD - Department of Biomedical Engineering, University of Connecticut, Storrs,
Connecticut.
FAU - Yau, Anne
AU - Yau A
Connecticut.
AD - Brown University Medical School, Providence, Rhode Island.
FAU - Yu, Hongchuan
AU - Yu H
FAU - Kuhn, Liisa
AU - Kuhn L
Connecticut.
AD - Department of Biomedical Engineering, University of Connecticut, Farmington,
Connecticut.
FAU - Guo, Wei
AU - Guo W
AD - Beijing Friendship Hospital, Capital Medical University, Beijing, China.
FAU - Chen, Yupeng
AU - Chen Y
AUID- ORCID: 0000-0001-6940-6277
Connecticut.
LA - eng
GR - P30 GM122732/GM/NIGMS NIH HHS/United States
DEP - 20200110
TA - J Biomed Mater Res A
JT - Journal of biomedical materials research. Part A
JID - 101234237
SB - IM
EIN - J Biomed Mater Res A. 2021 Feb;109(2):265. PMID: 33283452
PMC - PMC7207220
MID - NIHMS1576390
OTO - NOTNLM
OT - *Janus-based nanotubes
OT - *Nano-Matrix
OT - *anchorage
OT - *fibronectin
OT - *mesenchymal stem cells
COIS- CONFLICT OF INTEREST Y.C. is a cofounder of NanoDe Therapeutics, Inc.
EDAT- 2020/01/07 06:00
MHDA- 2020/01/07 06:00
CRDT- 2020/01/07 06:00
PHST- 2019/09/24 00:00 [received]
PHST- 2019/12/27 00:00 [revised]
PHST- 2019/12/31 00:00 [accepted]
PHST- 2020/01/07 06:00 [pubmed]
PHST- 2020/01/07 06:00 [medline]
PHST- 2020/01/07 06:00 [entrez]
AID - 10.1002/jbm.a.36875 [doi]
PST - ppublish
SO - J Biomed Mater Res A. 2020 Apr;108(4):984-991. doi: 10.1002/jbm.a.36875. Epub
2020
Jan 10.
PMID- 28653048
OWN - NLM
STAT- MEDLINE
DCOM- 20170821
LR - 20210227
IS - 2198-591X (Print)
VI - 12
IP - 2
DP - 2017
TI - Porcelain veneer post-bonding crack repair by resin infiltration.
PG - 156-170
AB - Ceramic laminate veneer restorations are indicated in several clinical
situations.
Indirect restorations are usually chosen if the less-invasive options -
bleaching,
resin infiltration, or composite resin restorations - are not possible, or
when it
is too difficult to achieve an esthetically pleasing result in the long term.
Bonded
indirect partial restorations are highly dependent on their adhesive
interface, as
these thin restorations have a relatively low cohesive strength. Therefore,
preservation of sound enamel, conditioning of the restorations and of the
substrate,
and luting procedures are of paramount importance for a successful outcome.
Even
when utmost care is taken during every step of the procedure, failures such
as
fractures, chipping, or marginal discoloration and defects sometimes occur.
Only
very few of these cases of failure are presented or are a subject of
interest. In
this case presentation, a fracture repair is performed using an infiltration
technique with a resin composite material.
FAU - Gresnigt, Marco
AU - Gresnigt M
FAU - Magne, Michel
AU - Magne M
FAU - Magne, Pascal
AU - Magne P
LA - eng
PT - Case Reports
PL - Germany
TA - Int J Esthet Dent
JT - The international journal of esthetic dentistry
JID - 101624416
RN - 0 (Dental Cements)
RN - 0 (Dental Materials)
RN - 0 (Monobond S)
RN - 0 (OptiBond FL)
RN - 179240-22-3 (Variolink)
SB - D
SB - IM
MH - Adult
MH - Composite Resins/*chemistry
MH - Dental Cements
MH - Dental Etching/methods
MH - Dental Materials/*chemistry
MH - Dental Polishing
MH - Dental Porcelain/*chemistry
MH - *Dental Restoration Failure
MH - *Dental Veneers
MH - *Esthetics, Dental
MH - Female
MH - Humans
MH - Incisor
MH - Maxilla
MH - Resin Cements
MH - Retreatment
EDAT- 2017/06/28 06:00
MHDA- 2017/08/22 06:00
CRDT- 2017/06/28 06:00
PHST- 2017/06/28 06:00 [entrez]
PHST- 2017/06/28 06:00 [pubmed]
PHST- 2017/08/22 06:00 [medline]
AID - 852285 [pii]
PST - ppublish
SO - Int J Esthet Dent. 2017;12(2):156-170.
PMID- 30981346
OWN - NLM
STAT- MEDLINE
DCOM- 20190729
LR - 20190729
IS - 0144-8617 (Linking)
VI - 215
DP - 2019 Jul 1
TI - Biomimetic chitosan-graft-polypeptides for improved adhesion in tissue and
metal.
PG - 20-28
LID - S0144-8617(19)30337-6 [pii]
LID - 10.1016/j.carbpol.2019.03.065 [doi]
AB - Inspired by the mussel foot protein and chitosan-based macromolecular
adhesives, a
series of chitosan-graft-polypeptides were synthesized by ring-opening
polymerization of three N-carboxyanhydrides (NCAs) -
3,4-dihydroxyphenylalanine-N-carboxyanhydride (DOPA-NCA), cysteine-NCA (Cys-
NCA) and
arginine-NCA (Arg-NCA) - using partial-NH(2)-protected chitosan as an
initiator.
These copolymers demonstrated good biodegradability and low cytotoxicity. The
results of lap-shear adhesion test showed that the maximum lap-shear adhesion
strength on the porcine skin and aluminum sheet were 195.97 ± 21.1 kPa and
3080 ± 320 kPa, respectively, and the maximum tensile adhesion strength on
bone was
642.70 ± 61.1 kPa. The rat experiment in vivo showed that these copolymers
exhibited
good hemostatic performance and can promote the healing of skin wound and
bone
fracture. It is expected that thesecopolymeric adhesives will have broad
applications in hemostasis and soft tissue adhesions.
CI - Copyright © 2019 Elsevier Ltd. All rights reserved.
FAU - Lu, Dedai
AU - Lu D
AD - Key Laboratory of Eco-Environment-Related Polymer Materials Ministry of
Education,
Key Laboratory of Polymer Materials of Gansu Province, College of Chemistry
and
Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China.
Electronic address: ludedai@nwnu.edu.cn.
FAU - Wang, Hongsen
AU - Wang H
Education,
and
FAU - Wang, Xiangya
AU - Wang X
Education,
and
FAU - Li, Yunfei
AU - Li Y
Education,
and
FAU - Guo, Hongyun
AU - Guo H
AD - Institute of Gansu Medical Science Research, Gansu Provincial Cancer
Hospital,
Lanzhou, 730050, China.
FAU - Sun, Shaobo
AU - Sun S
AD - School of Basic Medical Sciences, Gansu University of Chinese Medicine,
Lanzhou,
730000, China.
FAU - Zhao, Xiaolong
AU - Zhao X
Education,
and
FAU - Yang, Zhiwang
AU - Yang Z
Education,
and
FAU - Lei, Ziqiang
AU - Lei Z
Education,
and
LA - eng
DEP - 20190322
PL - England
TA - Carbohydr Polym
JT - Carbohydrate polymers
JID - 8307156
RN - 0 (Adhesives)
RN - 0 (Hemostatics)
RN - 0 (Peptides)
RN - 0 (Tissue Adhesives)
RN - 789U1901C5 (Copper)
RN - 9012-76-4 (Chitosan)
RN - CPD4NFA903 (Aluminum)
MH - Adhesives/chemical synthesis/*chemistry/pharmacology/therapeutic use
MH - Aluminum/chemistry
MH - Animals
MH - Biomimetic Materials/chemical synthesis/*chemistry/pharmacology/therapeutic
use
MH - Chitosan/*analogs & derivatives
MH - Copper/chemistry
MH - Fractures, Bone/therapy
MH - Hemostasis/drug effects
MH - Hemostatics/chemical synthesis/*chemistry/pharmacology/therapeutic use
MH - Male
MH - Peptides/chemical synthesis/*chemistry/pharmacology/therapeutic use
MH - Polymerization
MH - Rats
MH - Swine
MH - Tissue Adhesives/chemical synthesis/*chemistry/pharmacology/therapeutic use
MH - Wound Healing/drug effects
OTO - NOTNLM
OT - Biocompatible
OT - Biomimetic adhesive
OT - Chitosan
OT - Polypeptide
OT - Surgical adhesive
EDAT- 2019/04/15 06:00
MHDA- 2019/07/30 06:00
CRDT- 2019/04/15 06:00
PHST- 2018/09/15 00:00 [received]
PHST- 2019/03/14 00:00 [revised]
PHST- 2019/03/18 00:00 [accepted]
PHST- 2019/04/15 06:00 [entrez]
PHST- 2019/04/15 06:00 [pubmed]
PHST- 2019/07/30 06:00 [medline]
AID - S0144-8617(19)30337-6 [pii]
AID - 10.1016/j.carbpol.2019.03.065 [doi]
PST - ppublish
SO - Carbohydr Polym. 2019 Jul 1;215:20-28. doi: 10.1016/j.carbpol.2019.03.065.
Epub 2019
Mar 22.
PMID- 26721738
OWN - NLM
STAT- MEDLINE
DCOM- 20180119
LR - 20180314
IS - 0303-7207 (Linking)
VI - 432
DP - 2016 Sep 5
TI - Periostin action in bone.
PG - 75-82
LID - S0303-7207(15)30170-2 [pii]
LID - 10.1016/j.mce.2015.12.014 [doi]
AB - Periostin is a highly conserved matricellular protein that shares close
homology
with the insect cell adhesion molecule fasciclin 1. Periostin is expressed in
a
broad range of tissues including the skeleton, where it serves both as a
structural
molecule of the bone matrix and a signaling molecule through integrin
receptors and
Wnt-beta-catenin pathways whereby it stimulates osteoblast functions and bone
formation. The development of periostin null mice has allowed to elucidate

the
crucial role of periostin on dentinogenesis and osteogenesis, as well as on
the
skeletal response to mechanical loading and parathyroid hormone. The use of
circulating periostin as a potential clinical biomarker has been explored in
different non skeletal conditions. These include cancers and more
specifically in
the metastasis process, respiratory diseases such as asthma, kidney failure,
renal
injury and cardiac infarction. In postmenopausal osteoporosis, serum levels
have
been shown to predict the risk of fracture-more specifically non-vertebral-
independently of bone mineral density. Because of its preferential
localization in
cortical bone and periosteal tissue, it can be speculated that serum
periostin may
be a marker of cortical bone metabolism, although additional studies are
clearly
needed.
CI - Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Bonnet, Nicolas
AU - Bonnet N
AD - Division of Bone Diseases, Department of Internal Medicine Specialties,
Geneva
University Hospitals & Faculty of Medicine, Geneva 14, Switzerland.
Electronic
address: nicolas.bonnet@unige.ch.
FAU - Garnero, Patrick
AU - Garnero P
Geneva
FAU - Ferrari, Serge
AU - Ferrari S
Geneva
LA - eng
PT - Review
DEP - 20151222
PL - Ireland
TA - Mol Cell Endocrinol
JT - Molecular and cellular endocrinology
JID - 7500844
RN - 0 (Biomarkers)
RN - 0 (Parathyroid Hormone)
SB - IM
MH - Animals
MH - Biomarkers/metabolism
MH - Bone and Bones/drug effects/*metabolism
MH - Cell Adhesion Molecules/chemistry/*metabolism
MH - Humans
MH - Parathyroid Hormone/pharmacology
MH - Signal Transduction/drug effects
OTO - NOTNLM
OT - *Biomarker
OT - *Bone
OT - *Exercise
OT - *Fracture
OT - *PTH
OT - *Periostin
EDAT- 2016/01/02 06:00
MHDA- 2018/01/20 06:00
CRDT- 2016/01/02 06:00
PHST- 2015/11/26 00:00 [received]
PHST- 2015/12/17 00:00 [revised]
PHST- 2015/12/18 00:00 [accepted]
PHST- 2016/01/02 06:00 [entrez]
PHST- 2016/01/02 06:00 [pubmed]
PHST- 2018/01/20 06:00 [medline]
AID - S0303-7207(15)30170-2 [pii]
AID - 10.1016/j.mce.2015.12.014 [doi]
PST - ppublish
SO - Mol Cell Endocrinol. 2016 Sep 5;432:75-82. doi: 10.1016/j.mce.2015.12.014.
Epub 2015
Dec 22.
PMID- 23553771
OWN - NLM
STAT- MEDLINE
DCOM- 20130903
LR - 20130621
IS - 0736-0266 (Linking)
VI - 31
IP - 8
DP - 2013 Aug
TI - Autologous uncultured bone marrow-derived mononuclear cells and modified
cannulated
screw in repair of femoral neck fracture.
PG - 1302-7
LID - 10.1002/jor.22346 [doi]
AB - The purpose of this study was to assess whether autologous uncultured bone
marrow-derived mononuclear cells (BMMNCs) combined with modified cannulated
screw
would accelerate the healing of canine femoral neck fracture. BMMNCs were
encapsulated within fibrin glue (FG) and implanted into the fractured femoral
neck
via modified cannulated screw in experiment group, and the control group was
treated
by modified cannulated screw. Gross observation, radiological examination,
histological analysis, and blood vessel microdensity counting were used to
compare
bone healing of each group at 1, 2, and 3 months. FG was confirmed as an
ideal
cell-delivery vehicle for BMMNCs proliferation and differentiation in vitro
testing.
In vivo animal testing, faster new bone formation and fracture healing were
confirmed by gross observation, radiological examination, histological
analysis in
experimental group than in control group at all times points. The blood
vessel
microdensity counting increased gradually both in the experimental group and
control
group, but was more obviously in experimental group at 3 months (p < 0.01).
These
data suggest that autologous BMMNCs combined with modified cannulated screw
treatment is an effective therapy for femoral neck fracture and thus, may be
an
option for clinical applications.
CI - Copyright © 2013 Orthopaedic Research Society.
FAU - Licheng, Zhang
AU - Licheng Z
AD - Department of Orthopaedics, Chinese PLA General Hospital, Beijing, China.
FAU - Lihai, Zhang
AU - Lihai Z
FAU - Meng, Xu
AU - Meng X
FAU - Qi, Yao
AU - Qi Y
FAU - Peifu, Tang
AU - Peifu T
LA - eng
DEP - 20130328
PL - United States
TA - J Orthop Res
JT - Journal of orthopaedic research : official publication of the Orthopaedic
Research
Society
JID - 8404726
RN - 0 (Fibrin Tissue Adhesive)
SB - IM
MH - Animals
MH - Bone Marrow Transplantation
MH - *Bone Screws
MH - Cell Differentiation/drug effects
MH - Cell Proliferation/drug effects
MH - Combined Modality Therapy
MH - Dogs
MH - Femoral Neck Fractures/pathology/*therapy
MH - Fibrin Tissue Adhesive/*pharmacology
MH - Fracture Healing/*drug effects
MH - Fractures, Closed/pathology/*therapy
MH - Male
MH - Microcirculation
MH - Monocytes/pathology/*transplantation
MH - Transplantation, Autologous
EDAT- 2013/04/05 06:00
MHDA- 2013/09/04 06:00
CRDT- 2013/04/05 06:00
PHST- 2012/11/04 00:00 [received]
PHST- 2013/02/21 00:00 [accepted]
PHST- 2013/04/05 06:00 [entrez]
PHST- 2013/04/05 06:00 [pubmed]
PHST- 2013/09/04 06:00 [medline]
AID - 10.1002/jor.22346 [doi]
PST - ppublish
SO - J Orthop Res. 2013 Aug;31(8):1302-7. doi: 10.1002/jor.22346. Epub 2013 Mar
28.
PMID- 28921632
OWN - NLM
STAT- MEDLINE
DCOM- 20190319
LR - 20190319
IS - 0736-0266 (Print)
IS - 0736-0266 (Linking)
VI - 36
IP - 2
DP - 2018 Feb
TI - Elevated solute transport at sites of diffuse matrix damage in cortical bone:
Implications on bone repair.

PG - 692-698
LID - 10.1002/jor.23742 [doi]
AB - Diffuse matrix damage in rat cortical bone has been observed to self-repair
efficiently in 2 weeks without activating bone remodeling, and unlike the
case with
linear cracks, the local osteocytes at the sites of diffuse damage remain
healthy.
However, the reason(s) for such high efficiency of matrix repair remains
unclear. We
hypothesized that transport of minerals and other compounds essential for
damage
repair is enhanced at the damaged sites and further increased by the
application of
tensile loading. To test our hypothesis, diffuse damage was introduced in
notched
bovine wafers under cyclic tensile loading and unloading. Using the
Fluorescence
Recovery After Photobleaching (FRAP) approach, we measured the transport of a
small
fluorescent tracer (sodium fluorescein, 376 Da) in damaged versus undamaged
regions
and under varying tensile load magnitudes (0.2 N, 10 N, 20 N, and 30 N),
which
corresponded to nominal strains of 12.5, 625, 1,250, and 1,875 microstrains,
respectively. We found a 37% increase in transport of fluorescein in damaged
regions
relative to undamaged regions and a further ∼18% increase in transport under
20 N
and 30 N tension compared to the non-loaded condition, possibly due to the
opening
of the cracking surfaces. The elevated transport of minerals and other
adhesive
proteins may, at least partially, account for the highly effective repair of
diffuse
damage observed in vivo. CLINICAL SIGNIFICANCE: Diffuse damage adversely
affects
bone's fracture resistance and this study provided quantitative data on
elevated
transport, which may be involved in repairing diffuse damage in vivo. 2017
Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop
Res
36:692-698, 2018.
CI - © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
FAU - Wang, Bin
AU - Wang B
AUID- ORCID: 0000-0003-0225-9393
AD - Department of Mechanical Engineering, University of Delaware, Newark,
Delaware
19716.
AD - Institute of Life Sciences, Chongqing Medical University, Chongqing 400016,
P.R.
China.
FAU - Sun, Xuanhao
AU - Sun X
AD - Department of Physiology and Neurobiology, University of Connecticut, Storrs,
Connecticut 06269.
FAU - Akkus, Ozan
AU - Akkus O
AD - Department of Mechanical and Aerospace Engineering, and Biomedical
Engineering, Case
Western Reserve University, Cleveland, Ohio 44106.
FAU - Wang, Liyun
AU - Wang L
AD - Department of Mechanical Engineering, University of Delaware, Newark,
Delaware
19716.
LA - eng
GR - P30 GM103333/GM/NIGMS NIH HHS/United States
PT - Comparative Study
DEP - 20171116
TA - J Orthop Res
Research
Society
JID - 8404726
SB - IM
MH - Animals
MH - Bone Matrix/*injuries/*metabolism
MH - Cattle
MH - Fluorescence Recovery After Photobleaching
MH - Microscopy, Confocal
MH - Weight-Bearing
PMC - PMC5839948
MID - NIHMS907373
OTO - NOTNLM
OT - *damage repair
OT - *diffuse matrix damage
OT - *microdamage
OT - *osteocyte
OT - *solute transport
COIS- Conflict of Interest Statement: The authors declare no potential conflict of
interest. All authors have read and approved the final submitted manuscript.
EDAT- 2017/09/19 06:00
MHDA- 2019/03/20 06:00
CRDT- 2017/09/19 06:00
PHST- 2017/05/01 00:00 [received]
PHST- 2017/09/13 00:00 [accepted]
PHST- 2017/09/19 06:00 [pubmed]
PHST- 2019/03/20 06:00 [medline]
PHST- 2017/09/19 06:00 [entrez]
AID - 10.1002/jor.23742 [doi]
PST - ppublish
SO - J Orthop Res. 2018 Feb;36(2):692-698. doi: 10.1002/jor.23742. Epub 2017 Nov
16.
PMID- 31882795
OWN - NLM
STAT- MEDLINE
DCOM- 20201207
LR - 20210110
IS - 2045-2322 (Linking)
VI - 9
IP - 1
DP - 2019 Dec 27
TI - Potential of electrospun cationic BSA fibers to guide osteogenic MSC
differentiation
via surface charge and fibrous topography.
PG - 20003
LID - 10.1038/s41598-019-56508-6 [doi]
LID - 20003
AB - Large or complex bone fractures often need clinical treatments for sufficient
bone
repair. New treatment strategies have pursued the idea of using mesenchymal
stromal
cells (MSCs) in combination with osteoinductive materials to guide
differentiation
of MSCs into bone cells ensuring complete bone regeneration. To overcome the
challenge of developing such materials, fundamental studies are needed to
analyze
and understand the MSC behavior on modified surfaces of applicable materials
for
bone healing. For this purpose, we developed a fibrous scaffold resembling
the
bone/bone marrow extracellular matrix (ECM) based on protein without addition
of
synthetic polymers. With this biomimetic in vitro model we identified the
fibrous
structure as well as the charge of the material to be responsible for its
effects
on MSC differentiation. Positive charge was introduced via cationization that
additionally supported the stability of the scaffold in cell culture, and

acted as
nucleation point for mineralization during osteogenesis. Furthermore, we
revealed
enhanced focal adhesion formation and osteogenic differentiation of MSCs
cultured on
positively charged protein fibers. This pure protein-based and chemically
modifiable, fibrous ECM model allows the investigation of MSC behavior on
biomimetic
materials to unfold new vistas how to direct cells' differentiation for the
development of new bone regenerating strategies.
FAU - Raic, Annamarija
AU - Raic A
AD - Leibniz University Hannover, Institute of Cell Biology and Biophysics,
Hannover,
30419, Germany.
AD - Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces,
Eggenstein-Leopoldshafen, 76344, Germany.
FAU - Friedrich, Frank
AU - Friedrich F
AD - Karlsruhe Institute of Technology (KIT), Competence Center for Material
Moisture,
FAU - Kratzer, Domenic
AU - Kratzer D
Hannover,
30419, Germany.
FAU - Bieback, Karen
AU - Bieback K
AD - Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim,
Heidelberg University; German Red Cross Blood Service Baden-Württemberg -
Hessen,
Mannheim, 68167, Germany.
FAU - Lahann, Joerg
AU - Lahann J
AD - Biointerfaces Institute and Departments of Chemical Engineering, Materials
Science
and Engineering, Macromolecular Science and Engineering and Biomedical
Engineering,
University of Michigan, Ann Arbor, MI, 48109, USA.
FAU - Lee-Thedieck, Cornelia
AU - Lee-Thedieck C
AUID- ORCID: 0000-0001-8863-5403
Hannover,
30419, Germany. lee-thedieck@cell.uni-hannover.de.
LA - eng
DEP - 20191227
TA - Sci Rep
JT - Scientific reports
JID - 101563288
RN - 0 (Cations)
RN - 27432CM55Q (Serum Albumin, Bovine)
SB - IM
MH - Biomimetic Materials
MH - Cations
MH - *Cell Differentiation
MH - Electrochemistry/*methods
MH - Humans
MH - Mesenchymal Stem Cells/*cytology
MH - *Osteogenesis
MH - Proteolysis
MH - Serum Albumin, Bovine/*chemistry
MH - Static Electricity
PMC - PMC6934613
COIS- The authors declare no competing interests.
EDAT- 2019/12/29 06:00
MHDA- 2020/12/15 06:00
CRDT- 2019/12/29 06:00
PHST- 2019/04/18 00:00 [received]
PHST- 2019/12/12 00:00 [accepted]
PHST- 2019/12/29 06:00 [entrez]
PHST- 2019/12/29 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
AID - 10.1038/s41598-019-56508-6 [pii]
AID - 56508 [pii]
AID - 10.1038/s41598-019-56508-6 [doi]
PST - epublish
SO - Sci Rep. 2019 Dec 27;9(1):20003. doi: 10.1038/s41598-019-56508-6.
PMID- 30268514
OWN - NLM
STAT- MEDLINE
DCOM- 20190523
LR - 20190523
IS - 0020-1383 (Linking)
VI - 49
IP - 12
DP - 2018 Dec
TI - Use of collagen/chitosan sponges mineralized with hydroxyapatite for the
repair of
cranial defects in rats.
PG - 2154-2160
LID - S0020-1383(18)30520-5 [pii]
LID - 10.1016/j.injury.2018.09.018 [doi]
AB - In traumatology, we encounter several clinical challenges that involve
extensive
bone loss primarily related to trauma, conditions that can be treated with
autologous grafts. A good alternative is the use of synthetic biomaterials as
substitutes. These polymers provide a suitable environment for the growth of

new
bone and vascular tissue, which are essential for repair.
Collagen/hydroxyapatite
composites have proven to be biocompatible and to behave mechanically.
Furthermore,
the addition of chitosan contributes to the formation of a three-dimensional
structure that permits cell adhesion and proliferation, thus improving
osteogenesis.
The aim of this study was to evaluate bone formation during the repair of
bone
defects experimentally induced in the skull of rats and grafted with a
polymer blend
consisting of bovine tendon collagen and chitosan combined with
hydroxyapatite.
Thirty animals were used for the creation of a defect in the left parietal
bone and
were divided into three groups of 10 animals each: a control group without
biomaterial implantation, a group receiving the blend of collagen and
chitosan, and
a group receiving this blend combined with hydroxyapatite. Each group was
subdivided
and the animals were sacrificed 3 or 8 weeks after surgery. After sacrifice,
the
skulls were removed for macroscopic photodocumentation and radiographic
examination.
The samples were processed for histological evaluation of new bone formation
at the
surgical site. Macroscopic and radiographic analysis demonstrated the
biocompatibility of the blends. Histologically, the formation of new bone
occurred
in continuity with the edges of the defect, with the observation of higher
volumes
in the grafted groups compared to control. Mineralization of sponges did not
stimulate bone neoformation, with bone repair being incomplete over the
experimental
period. In conclusion, mineralization by the addition of hydroxyapatite
should be
better studied. However, the collagen/chitosan sponges used in this study are
suitable to stimulate osteogenesis in cranial defects, although this process

is slow
and not sufficient to achieve complete bone regeneration over a short period
of
time.
FAU - Munhoz, M A S
AU - Munhoz MAS
AD - Department of Morphology and Pathology, Faculty of Medicine of Jundiaí, Rua
Francisco Telles, 250, Vila Arens, Jundiaí, CEP. 13202-550, Cx. Postal 1295,
SP,
Brazil; Postgraduate Program Interunidades in Bioengineering (EESC/FMRP
/IQSC)
(EESC/FMRP/IQSC), University São Paulo, USP, Av. Trabalhador São Carlense,
400, São
Carlos-SP, Brazil. CEP: 13566-590.
FAU - Hirata, H H
AU - Hirata HH
SP,
/IQSC)
400, São
Carlos-SP, Brazil. CEP: 13566-590.
FAU - Plepis, A M G
AU - Plepis AMG
AD - Institute of Chemistry of São Carlos, IQSC, University São Paulo, USP, Av.
Trabalhador São Carlense, 400, São Carlos, SP, CEP: 13566-590, Brazil;
Postgraduate
Program Interunidades in Bioengineering (EESC/FMRP /IQSC) (EESC/FMRP/IQSC),
University São Paulo, USP, Av. Trabalhador São Carlense, 400, São Carlos-SP,
Brazil.
CEP: 13566-590.
FAU - Martins, V C A
AU - Martins VCA
AD - Institute of Chemistry of São Carlos, IQSC, University São Paulo, USP, Av.
Trabalhador São Carlense, 400, São Carlos, SP, CEP: 13566-590, Brazil.
FAU - Cunha, M R
AU - Cunha MR
SP,
/IQSC)
400, São
Carlos-SP, Brazil. CEP: 13566-590. Electronic address: cunhamr@hotmail.com.
LA - eng
DEP - 20180920
PL - Netherlands
TA - Injury
JT - Injury
JID - 0226040
RN - 0 (Bmp2 protein, rat)
RN - 0 (Bone Substitutes)
RN - 9007-34-5 (Collagen)
SB - IM
MH - Animals
MH - Bone Morphogenetic Protein 2
MH - Bone Substitutes/*chemistry
MH - Cell Adhesion
MH - Chitosan/*pharmacology
MH - Collagen/*pharmacology
MH - Durapatite/*pharmacology
MH - Male
MH - Osteogenesis/*physiology
MH - Rats
MH - Rats, Wistar
MH - Skull Fractures/*pathology/surgery
OTO - NOTNLM
OT - Chitosan
OT - Collagen
OT - Hydroxyapatite
OT - Polymers
EDAT- 2018/10/01 06:00
MHDA- 2019/05/24 06:00
CRDT- 2018/10/01 06:00
PHST- 2018/08/17 00:00 [received]
PHST- 2018/09/09 00:00 [accepted]
PHST- 2018/10/01 06:00 [pubmed]
PHST- 2019/05/24 06:00 [medline]
PHST- 2018/10/01 06:00 [entrez]
AID - S0020-1383(18)30520-5 [pii]
AID - 10.1016/j.injury.2018.09.018 [doi]
PST - ppublish
SO - Injury. 2018 Dec;49(12):2154-2160. doi: 10.1016/j.injury.2018.09.018. Epub
2018 Sep
20.
PMID- 31529455
OWN - NLM
STAT- MEDLINE
DCOM- 20200611
LR - 20200611
IS - 1473-2262 (Linking)
VI - 38
DP - 2019 Sep 17
TI - Complete regeneration of large bone defects in rats with commercially
available
fibrin loaded with BMP-2.
PG - 94-105
LID - 10.22203/eCM.v038a08 [doi]
AB - This study aimed at investigating in vitro and in vivo the efficiency of
commercially available fibrin as a carrier for controlled and sustained bone
morphogenetic protein-2 (BMP-2) release to induce bone formation and reduce
the side
effects of its use. In vitro release and activity of low-dose recombinant
human
BMP-2 (rhBMP-2) (37.5 µg/mL) embedded in commercially available fibrin were
evaluated and, subsequently, critical-size femur defects in rats were grafted
to
study bone regeneration and vascularisation by micro-computed tomography
(µCT) and
histology. In vitro experiments showed a sustained BMP-2 release with a high
BMP
activity remaining after 28 d. In vivo, fibrin loaded with BMP-2 showed an
extremely
fast bone healing, with a large amount of new bone formation throughout the
entire
defect in the first 4 weeks and complete cortical repair and fusion after 8
weeks,
with no ectopic bone formation. In contrast, the control fibrin group did not
fuse
after 12 weeks. Vascularisation was similar in both groups at 4 and 12 weeks
after
implantation. In conclusion, commercially available fibrin is a very
efficient
carrier for rhBMP-2 to graft critical-size cortical bone defects and might be
a more
optimal delivery vehicle for BMP-2-induced bone regeneration than currently
available collagen sponges.
FAU - Koolen, M
AU - Koolen M
AD - Department of Orthopaedics, UMC Utrecht, G.05.228, P.O. Box 85500, 3508 GA
Utrecht,
the Netherlands.m.k.e.koolen@umcutrecht.nl.
FAU - Longoni, A
AU - Longoni A
FAU - van der Stok, J
AU - van der Stok J
FAU - Van der Jagt, O
AU - Van der Jagt O
FAU - Gawlitta, D
AU - Gawlitta D
FAU - Weinans, H
AU - Weinans H
LA - eng
DEP - 20190917
PL - Switzerland
TA - Eur Cell Mater
JT - European cells & materials
JID - 100973416
RN - 0 (Hydrogels)
SB - IM
MH - Animals
MH - Bone Morphogenetic Protein 2/*pharmacology
MH - Bone Substitutes/adverse effects/*chemistry
MH - Cell Line
MH - Drug Liberation
MH - Femoral Fractures/*therapy
MH - Femur/drug effects
MH - Humans
MH - Hydrogels/adverse effects/chemistry
MH - Mice
MH - Rats
MH - Rats, Wistar
EDAT- 2019/09/19 06:00
MHDA- 2020/06/12 06:00
CRDT- 2019/09/19 06:00
PHST- 2019/09/19 06:00 [entrez]
PHST- 2019/09/19 06:00 [pubmed]
PHST- 2020/06/12 06:00 [medline]
AID - vol038a08 [pii]
AID - 10.22203/eCM.v038a08 [doi]
PST - epublish
SO - Eur Cell Mater. 2019 Sep 17;38:94-105. doi: 10.22203/eCM.v038a08.
PMID- 30772515
OWN - NLM
STAT- MEDLINE
DCOM- 20200416
LR - 20200416
IS - 1742-7061 (Linking)
VI - 88
DP - 2019 Apr 1
TI - A strong, tough, and osteoconductive hydroxyapatite mineralized
polyacrylamide/dextran hydrogel for bone tissue regeneration.
PG - 503-513
LID - S1742-7061(19)30123-0 [pii]
LID - 10.1016/j.actbio.2019.02.019 [doi]
AB - The design of hydrogels with adequate mechanical properties and excellent
bioactivity, osteoconductivity, and capacity for osseointegration is
essential to
bone repair and regeneration. However, it is challenging to integrate all
these
properties into one bone scaffold. Herein, we developed a strong, tough,
osteoconductive hydrogel by a facile one-step micellar copolymerization of
acrylamide and urethacrylate dextran (Dex-U), followed by the in situ
mineralization
of hydroxyapatite (HAp) nanocrystals. We show that the soft, flexible, and
hydrophobically associated polyacrylamide (PAAm) network is strengthened by
the
stiff crosslinked Dex-U phase, and that the mineralized HAp simultaneously
improves
the mechanical properties and osteoconductivity. The obtained HAp mineralized
PAAm/Dex-U hydrogel (HAp-PADH) has extremely high compressive strength

(6.5 MPa) and
enhanced fracture resistance (over 2300 J m(-2)), as compared with pure PAAm
hydrogels. In vitro, we show that the mineralized HAp layer promotes the
adhesion
and proliferation of osteoblasts, and effectively stimulates osteogenic
differentiation. Through the in vivo evaluation of hydrogels in a femoral
condyle
defect rabbit model, we show regeneration of a highly mineralized bone tissue
and
direct bonding to the HAp-PADH interface. These findings confirm the
excellent
osteoconductivity and osseointegration ability of fabricated HAp-PADH. The
present
HAp-PADH, with its superior mechanical properties and excellent
osteoconductivity,
should have great potential for bone repair and regeneration. STATEMENT OF
SIGNIFICANCE: We developed a strong, tough, and osteoconductive hydrogel by a
facile
one-step micellar copolymerization of acrylamide and urethane methacrylate
dextran
(Dex-U), followed by the in situ mineralization of hydroxyapatite (HAp)
nanocrystals. The hydrophobic micellar copolymerization and introduction of
the
stiff crosslinked Dex-U phase endowed the soft polyacrylamide (PAAm) network
with
enhanced strength and toughness. The in situ mineralized HAp nanocrystals on
the
hydrogels further improved the mechanical properties of the hydrogels and
promoted
osteogenic differentiation of cells. Mechanical tests together with in vitro
and in
vivo evaluations confirmed that the HAp mineralized PAAm/Dex-U hydrogel (HAp-
PADH)
achieved a combination of superior mechanical properties and excellent
osseointegration, and thus may offer a promising candidate for bone repair
and
regeneration.
CI - Copyright © 2019 Acta Materialia Inc. Published by Elsevier Ltd. All rights
reserved.
FAU - Fang, Ju
AU - Fang J
AD - Department of Materials Science and Engineering, Southern University of
Science and
Technology, Shenzhen, Guangdong 518055, China; State Key Laboratory of
Bioelectronics, Southeast University, Nanjing, Jiangsu 210096, China.
FAU - Li, Pengfei
AU - Li P
AD - Key Lab of Advanced Technologies of Materials, Ministry of Education, School
of
Materials Science and Engineering, Southwest Jiaotong University, Chengdu,
Sichuan
621000, China.
FAU - Lu, Xiong
AU - Lu X
AD - Key Lab of Advanced Technologies of Materials, Ministry of Education, School
of
Materials Science and Engineering, Southwest Jiaotong University, Chengdu,
Sichuan
621000, China.
FAU - Fang, Liming
AU - Fang L
AD - National Engineering Research Center for Tissue Restoration and
Reconstruction,
South China University of Technology, Guangzhou, Guangdong 510006, China.
FAU - Lü, Xiaoying
AU - Lü X
AD - State Key Laboratory of Bioelectronics, Southeast University, Nanjing,
Jiangsu
210096, China.
FAU - Ren, Fuzeng
AU - Ren F
AD - Department of Materials Science and Engineering, Southern University of
Science and
Technology, Shenzhen, Guangdong 518055, China. Electronic address:
renfz@sustc.edu.cn.
LA - eng
DEP - 20190214
PL - England
TA - Acta Biomater
JT - Acta biomaterialia
JID - 101233144
RN - 0 (Acrylic Resins)
RN - 0 (Dextrans)
RN - 0 (Hydrogels)
RN - 9003-05-8 (polyacrylamide)
SB - IM
MH - Acrylic Resins/*pharmacology
MH - Animals
MH - Bone and Bones/*physiology
MH - Calcification, Physiologic/*physiology
MH - Cell Adhesion/drug effects
MH - Cell Line
MH - Dextrans/*pharmacology
MH - Female
MH - Hydrogels/*pharmacology
MH - Mice
MH - Osseointegration/drug effects
MH - Osteoblasts/cytology/drug effects
MH - Rabbits
MH - X-Ray Microtomography
OTO - NOTNLM
OT - *Bone regeneration
OT - *Osseointegration
OT - *Osteoconductive
OT - *Tough hydrogel
EDAT- 2019/02/18 06:00
MHDA- 2020/04/17 06:00
CRDT- 2019/02/18 06:00
PHST- 2018/11/05 00:00 [received]
PHST- 2019/02/12 00:00 [revised]
PHST- 2019/02/13 00:00 [accepted]
PHST- 2019/02/18 06:00 [pubmed]
PHST- 2020/04/17 06:00 [medline]
PHST- 2019/02/18 06:00 [entrez]
AID - S1742-7061(19)30123-0 [pii]
AID - 10.1016/j.actbio.2019.02.019 [doi]
PST - ppublish
SO - Acta Biomater. 2019 Apr 1;88:503-513. doi: 10.1016/j.actbio.2019.02.019. Epub
2019
Feb 14.
PMID- 30834821
OWN - NLM
STAT- MEDLINE
DCOM- 20200723
LR - 20200723
IS - 1937-3384 (Linking)
VI - 25
IP - 5
DP - 2019 May
TI - Biocompatible Three-Dimensional Printed Thermoplastic Scaffold for Osteoblast
Differentiation of Equine Induced Pluripotent Stem Cells.

PG - 253-261
LID - 10.1089/ten.TEC.2018.0343 [doi]
AB - Horses, like humans, can experience bone fractures and due to their large
size and
the need to bear weight on all limbs during the recovery period, they can be
difficult to treat. Surgical techniques to improve fracture repair are
improving,
but to date, regenerative medicine technologies to aid fracture healing are
not
commonly applied in horses. We have previously demonstrated that equine
induced
pluripotent stem cells (iPSCs) can be differentiated into bone forming
osteoblasts
in 2D culture. In this study, we report on the use of a thermoplastic, 3D-
printed
polymer to provide a scaffold for successful, in vitro osteoblast
differentiation of
equine iPSCs. The scaffold provides a transparent, cost-effective solution to
allow
the analysis of osteoblast differentiation using live-cell imaging,
immunohistochemistry, and quantitative polymerase chain reaction. This in
vitro
study demonstrates the future feasibility of generating 3D bone constructs
through
the cell seeding of scaffolds to use in regenerative medicine strategies to
improve
fracture repair in a relevant, large animal model.
FAU - Baird, Arabella
AU - Baird A
AD - 1 Centre for Preventive Medicine, Animal Health Trust, Newmarket, United
Kingdom.
FAU - Dominguez Falcon, Noelia
AU - Dominguez Falcon N
AD - 2 School of Pharmacy, University of East Anglia, Norwich, United Kingdom.
FAU - Saeed, Aram
AU - Saeed A
AD - 2 School of Pharmacy, University of East Anglia, Norwich, United Kingdom.
FAU - Guest, Deborah Jane
AU - Guest DJ
AD - 1 Centre for Preventive Medicine, Animal Health Trust, Newmarket, United
Kingdom.
LA - eng
PL - United States
TA - Tissue Eng Part C Methods
JT - Tissue engineering. Part C, Methods
JID - 101466663
SB - IM
MH - 3T3 Cells
MH - Animals
MH - Biocompatible Materials/*pharmacology
MH - Calcification, Physiologic/drug effects
MH - *Cell Differentiation/drug effects
MH - Extracellular Matrix/drug effects/metabolism
MH - Fibroblasts/cytology/drug effects
MH - Gene Expression Regulation/drug effects
MH - Horses
MH - Humans
MH - Induced Pluripotent Stem Cells/*cytology/drug effects
MH - Mice
MH - Osteoblasts/*cytology/drug effects/metabolism
MH - *Printing, Three-Dimensional
MH - *Temperature
OTO - NOTNLM
OT - *3D printing
OT - *bone differentiation
OT - *induced pluripotent stem cells
OT - *scaffold
EDAT- 2019/03/06 06:00
MHDA- 2020/07/24 06:00
CRDT- 2019/03/06 06:00
PHST- 2019/03/06 06:00 [pubmed]
PHST- 2020/07/24 06:00 [medline]
PHST- 2019/03/06 06:00 [entrez]
AID - 10.1089/ten.TEC.2018.0343 [doi]
PST - ppublish
SO - Tissue Eng Part C Methods. 2019 May;25(5):253-261. doi:
10.1089/ten.TEC.2018.0343.
PMID- 32764276
OWN - NLM
STAT- MEDLINE
DCOM- 20210301
LR - 20210301
IS - 1424-8220 (Linking)
VI - 20
IP - 16
DP - 2020 Aug 5
TI - Characterization and Monitoring of Titanium Bone Implants with Impedance
Spectroscopy.
LID - 10.3390/s20164358 [doi]
LID - 4358
AB - Porous titanium is a metallic biomaterial with good properties for the
clinical
repair of cortical bone tissue, although the presence of pores can compromise
its
mechanical behavior and clinical use. It is therefore necessary to
characterize the
implant pore size and distribution in a suitable way. In this work, we
explore the
new use of electrical impedance spectroscopy for the characterization and
monitoring
of titanium bone implants. Electrical impedance spectroscopy has been used as
a
non-invasive route to characterize the volumetric porosity percentage (30%,
40%, 50%
and 60%) and the range of pore size (100-200 and 355-500 mm) of porous
titanium
samples obtained with the space-holder technique. Impedance spectroscopy is
proved
to be an appropriate technique to characterize the level of porosity of the
titanium
samples and pore size, in an affordable and non-invasive way. The technique
could
also be used in smart implants to detect changes in the service life of the
material, such as the appearance of fractures, the adhesion of osteoblasts
and
bacteria, or the formation of bone tissue.
FAU - Olmo, Alberto
AU - Olmo A
AUID- ORCID: 0000-0001-6388-4462
AD - Instituto de Microelectrónica de Sevilla, IMSE-CNM (CSIC, Universidad de
Sevilla),
Av. Américo Vespucio, sn, 41092 Sevilla, Spain.
AD - Escuela Técnica Superior de Ingeniería Informática, Departamento de
Tecnología
Electrónica, Universidad de Sevilla, Av. Reina Mercedes sn, 41012 Sevilla,
Spain.
FAU - Hernández, Miguel
AU - Hernández M
AD - Instituto de Microelectrónica de Sevilla, IMSE-CNM (CSIC, Universidad de
Sevilla),
Av. Américo Vespucio, sn, 41092 Sevilla, Spain.
AD - Escuela Técnica Superior de Ingeniería Informática, Departamento de
Tecnología
Electrónica, Universidad de Sevilla, Av. Reina Mercedes sn, 41012 Sevilla,
Spain.
FAU - Chicardi, Ernesto
AU - Chicardi E
AD - Departamento de Ingeniería y Ciencia de los Materiales y del Transporte,
Escuela
Superior de Ingenieros, Universidad de Sevilla, 41092 Sevilla, Spain.
FAU - Torres, Yadir
AU - Torres Y
AUID- ORCID: 0000-0002-6481-0438
AD - Departamento de Ingeniería y Ciencia de los Materiales y del Transporte,
Escuela
Politécnica Superior, Calle Virgen de África, 7, 41011 Sevilla, Spain.
LA - eng
GR - US-1259771/Junta de Andalucía/
DEP - 20200805
TA - Sensors (Basel)
JT - Sensors (Basel, Switzerland)
JID - 101204366
SB - IM
MH - Bone and Bones
MH - *Dielectric Spectroscopy
MH - Materials Testing
MH - Porosity
MH - *Prostheses and Implants
MH - *Titanium
PMC - PMC7472105
OTO - NOTNLM
OT - cortical bone tissue
OT - electrical impedance spectroscopy
OT - porous titanium
OT - smart implants
EDAT- 2020/08/09 06:00
MHDA- 2021/03/02 06:00
CRDT- 2020/08/09 06:00
PHST- 2020/06/16 00:00 [received]
PHST- 2020/07/27 00:00 [revised]
PHST- 2020/08/03 00:00 [accepted]
PHST- 2020/08/09 06:00 [entrez]
PHST- 2020/08/09 06:00 [pubmed]
PHST- 2021/03/02 06:00 [medline]
AID - s20164358 [pii]
AID - sensors-20-04358 [pii]
AID - 10.3390/s20164358 [doi]
PST - epublish
SO - Sensors (Basel). 2020 Aug 5;20(16):4358. doi: 10.3390/s20164358.
PMID- 32165194
OWN - NLM
STAT- MEDLINE
DCOM- 20210519
LR - 20210519
IS - 1742-7061 (Linking)
VI - 108
DP - 2020 May
TI - In vitro and in vivo studies of Zn-Mn biodegradable metals designed for
orthopedic
applications.
PG - 358-372
LID - S1742-7061(20)30141-0 [pii]
LID - 10.1016/j.actbio.2020.03.009 [doi]
AB - In recent years, Zn-based materials provide a new option as biodegradable
metals for
orthopedic applications. To improve the low strength and brittle nature of
pure Zn,
small amounts of alloying element Mn (0.1, 0.4 and 0.8 wt.%) were added into
Zn to
fabricate binary Zn-Mn alloys. An extremely high elongation (83.96 ± 2.36%)
was
achieved in the resulting Zn-0.8 wt.%Mn alloy. Moreover, Zn-Mn alloys
displayed
significantly improved cytocompatibility as compared to pure Zn, according to
cell
proliferation and morphology analyses. More importantly, a significantly
improved
osteogenic activity was verified after adding Mn regarding ALP activity and
osteogenic expression. Furthermore, Zn-0.8 wt.%Mn alloy scaffolds were
implanted
into the rat femoral condyle for repairing bone defects with pure Ti as
control.
Enhanced osteogenic activities were confirmed for Zn-0.8Mn alloy in contrast
to pure
Ti based on Micro-CT and histological results, and favorable in vivo
biosafety of
Zn-0.8Mn alloy was verified by H&E staining and blood tests. The exceptional
mechanical performance and favorable osteogenic capability render Zn-Mn alloy
a
promising candidate material in the treatment of bone defects or fracture
repair.
STATEMENT OF SIGNIFICANCE: The element Mn, on the one hand, as an essential
trace
element in the human body, promotes cell proliferation, adhesion, spreading,
and
regulates bone metabolism; on the other hand, it could significantly improve
the
ductility of Zn alloys. Here, we systematically reported the biocompatibility
and
biofunctionality of binary biodegradable Zn-Mn alloys in the bone
environment. The
Zn-Mn alloys promoted MC3T3-E1 cell proliferation, adhesion, spreading, and
osteogenic differentiation in vitro. Furthermore, a rat femoral condyle
defect model
was established; porous Zn-Mn alloy scaffolds were manufactured to repair the
bone
defects. Significant bone regenerations, considerable bone ingrowth, and
desirable
biosafety were confirmed in vivo. Therefore, biodegradable Zn-Mn with
promising
osteogenic properties may become new options for orthopedic implant
materials.
CI - Copyright © 2020. Published by Elsevier Ltd.
FAU - Jia, Bo
AU - Jia B
AD - Department of Orthopaedic Surgery, Shanghai Key Laboratory of Orthopaedic
Implants,
Shanghai Ninth People's Hospital, Shanghai Jiaotong University, School of
Medicine,
Shanghai 200011, PR China.
FAU - Yang, Hongtao
AU - Yang H
AD - Department of Materials Science and Engineering, College of Engineering,
Peking
University, Beijing 100871, PR China; Department of Plastic & Reconstructive
Surgery, The Ohio State University, Columbus, OH 43210, United States.
FAU - Han, Yu
AU - Han Y
Implants,
Medicine,
FAU - Zhang, Zechuan
AU - Zhang Z
Peking
University, Beijing 100871, PR China.
FAU - Qu, Xinhua
AU - Qu X
AD - Department of Bone and Joint Surgery, Renji Hospital, School of Medicine,
Shanghai
Jiao Tong University, Shanghai 200127, PR China.
FAU - Zhuang, Yifu
AU - Zhuang Y
Implants,
Medicine,
FAU - Wu, Qiang
AU - Wu Q
Implants,
Medicine,
FAU - Zheng, Yufeng
AU - Zheng Y
Peking
University, Beijing 100871, PR China. Electronic address: yfzheng@pku.edu.cn.
FAU - Dai, Kerong
AU - Dai K
Implants,
Medicine,
Shanghai 200011, PR China. Electronic address: krdai@163.com.
LA - eng
DEP - 20200309
PL - England
TA - Acta Biomater
JID - 101233144
RN - 0 (Alloys)
RN - J41CSQ7QDS (Zinc)
SB - IM
MH - *Absorbable Implants
MH - Alloys/pharmacology
MH - Animals
MH - Biocompatible Materials/pharmacology
MH - *Osteogenesis
MH - Rats
MH - Zinc/pharmacology
OTO - NOTNLM
OT - *Biodegradable metal
OT - *Orthopedic implant
OT - *Osteogenesis
OT - *Zn-Mn alloy
COIS- Declaration of Competing Interests The authors declare no competing financial
interests or personal relationships.

EDAT- 2020/03/14 06:00
MHDA- 2021/05/20 06:00
CRDT- 2020/03/14 06:00
PHST- 2019/12/06 00:00 [received]
PHST- 2020/03/01 00:00 [revised]
PHST- 2020/03/04 00:00 [accepted]
PHST- 2020/03/14 06:00 [pubmed]
PHST- 2021/05/20 06:00 [medline]
PHST- 2020/03/14 06:00 [entrez]
AID - S1742-7061(20)30141-0 [pii]
AID - 10.1016/j.actbio.2020.03.009 [doi]
PST - ppublish
SO - Acta Biomater. 2020 May;108:358-372. doi: 10.1016/j.actbio.2020.03.009. Epub
2020
Mar 9.
PMID- 28382553
OWN - NLM
STAT- MEDLINE
DCOM- 20180716
LR - 20210216
VI - 28
IP - 8
DP - 2017 Aug
TI - Circulating periostin levels increase in association with bone density loss
and
healing progression during the early phase of hip fracture in Chinese older
women.
PG - 2335-2341
LID - 10.1007/s00198-017-4034-z [doi]
AB - The present study shows that hip fracture women had higher serum periostin
(sPostn)
levels. The elevation of sPostn is associated with bone density loss, yet
fracture
itself may even increase sPostn levels during early healing phase.
INTRODUCTION: The
study aims to quantify the associations of sPostn levels with bone density
loss and
the possible effect on the fracture healing. METHODS: This study enrolled 261
older
women with osteoporotic hip fracture and 106 age-matched women without
fracture
serving as controls. Clinical features, bone mineral density (BMD), and bone
turnover markers including sPostn level were measured after fracture within
2 days.
Follow-up sPostn levels during 1 year after 2 days were available for 128
patients.
RESULTS: Initial levels of sPostn after fracture were significantly higher in
patients than controls. sPostn was correlated with BMD of femoral neck (r = -
0.529,
P < 0.001), β-isomerized C-terminal crosslinking of type I collagen (β-CTX)
(r = 0.403, P = 0.008), and N-terminal procollagen of type I collagen (PINP)
(r = 0.236, P = 0.042) in the entire cohort. After multivariate analysis,
sPostn
remained as an independent risk factor for femoral neck BMD, which explained
19.1%
of the variance in BMD. sPostn sampled within 7 days after fracture were
acutely
increasing from day 2 and then decreased and maintained at slightly high
levels at
360 days. The percentage changes of sPostn positively correlated with the
variation
in β-CTX (r = 0.396, P = 0.002) and PINP (r = 0.180, P = 0.033) at day 7
after
fracture. CONCLUSIONS: High sPostn levels were an independent predictor of
femoral
neck BMD in older women presenting with an acute hip fracture. Increased
sPostn
levels during early healing phase may imply that Postn play a role in bone
repair.
FAU - Yan, J
AU - Yan J
AD - Department of Orthopaedic Surgery, Liaocheng People's Hospital, Liaocheng,
Shandong,
China.
FAU - Liu, H J
AU - Liu HJ
AD - Department of Endocrinology, Liaocheng People's Hospital, Liaocheng,
Shandong,
China.
FAU - Li, H
AU - Li H
Shandong,
China.
FAU - Chen, L
AU - Chen L
Shandong,
China.
FAU - Bian, Y Q
AU - Bian YQ
Shandong,
China.
FAU - Zhao, B
AU - Zhao B
Shandong,
China.
FAU - Han, H X
AU - Han HX
AD - Second Department of Clinical Medicine, Medical University of Anhui, Hefei,
Anhui,
China.
FAU - Han, S Z
AU - Han SZ
Shandong,
China.
FAU - Han, L R
AU - Han LR
Shandong,
China. fuleco@126.com.
FAU - Wang, D W
AU - Wang DW
Shandong,
China. wdw62@sohu.com.
FAU - Yang, X F
AU - Yang XF
Shandong,
China.
LA - eng
DEP - 20170405
PL - England
TA - Osteoporos Int
JT - Osteoporosis international : a journal established as result of cooperation
between
the European Foundation for Osteoporosis and the National Osteoporosis
Foundation of
the USA
JID - 9100105
RN - 0 (Biomarkers)
SB - IM
CIN - Osteoporos Int. 2020 Oct;31(10):2061. PMID: 32827067
MH - Absorptiometry, Photon/methods
MH - Aged
MH - Aged, 80 and over
MH - Biomarkers/blood
MH - Bone Density/*physiology
MH - Case-Control Studies
MH - Cell Adhesion Molecules/*blood/physiology
MH - Female
MH - Femur Neck/physiopathology
MH - Hip Fractures/*blood/physiopathology
MH - Humans
MH - Lumbar Vertebrae/physiopathology
MH - Osteoporosis, Postmenopausal/*blood/physiopathology
MH - Osteoporotic Fractures/*blood/physiopathology
OTO - NOTNLM
OT - *Elderly
OT - *Fracture repair
OT - *Hip fracture
OT - *Osteoporosis
OT - *Periostin
EDAT- 2017/04/07 06:00
MHDA- 2018/07/17 06:00
CRDT- 2017/04/07 06:00
PHST- 2016/12/15 00:00 [received]
PHST- 2017/03/30 00:00 [accepted]
PHST- 2017/04/07 06:00 [pubmed]
PHST- 2018/07/17 06:00 [medline]
PHST- 2017/04/07 06:00 [entrez]
AID - 10.1007/s00198-017-4034-z [pii]
AID - 10.1007/s00198-017-4034-z [doi]
PST - ppublish
SO - Osteoporos Int. 2017 Aug;28(8):2335-2341. doi: 10.1007/s00198-017-4034-z.
Epub 2017
Apr 5.
PMID- 21132409
OWN - NLM
STAT- MEDLINE
DCOM- 20110930
LR - 20201209
IS - 0009-921X (Print)
VI - 469
IP - 8
DP - 2011 Aug
TI - Anabolic agents and bone quality.
PG - 2215-24
LID - 10.1007/s11999-010-1722-9 [doi]
AB - BACKGROUND: The definition of bone quality is evolving particularly from the
perspective of anabolic agents that can enhance not only bone mineral density
but
also bone microarchitecture, composition, morphology, amount of microdamage,
and
remodeling dynamics. QUESTIONS/PURPOSES: This review summarizes the molecular
pathways and physiologic effects of current and potential anabolic drugs.

METHODS:
From a MEDLINE search (1996-2010), articles were identified by the search
terms
"bone quality" (1851 articles), "anabolic agent" (5044 articles), "PTH or
parathyroid hormone" (32,229 articles), "strontium" or "strontium ranelate"
(283
articles), "prostaglandin" (77,539 articles), and "statin" or "statins"
(14,233
articles). The search strategy included combining each with the phrase "bone
quality." Another more limited search aimed at finding more novel potential
agents.
RESULTS: Parathyroid hormone is the only US Food and Drug Administration-
approved
bone anabolic agent in the United States and has been the most extensively
studied
in in vitro animal and human trials. Strontium ranelate is approved in Europe
but
has not undergone Food and Drug Administration trials in the United States.
All the
studies on prostaglandin agonists have used in vivo animal models and there
are no
human trials examining prostaglandin agonist effects. The advantages of
statins
include the long-established advantages and safety profile, but they are
limited by
their bioavailability in bone. Other potential pathways include proline-rich
tyrosine kinase 2 (PYK2) and sclerostin (SOST) inhibition, among others.
CONCLUSIONS: The ongoing research to enhance the anabolic potential of
current
agents, identify new agents, and develop better delivery systems will greatly
enhance the management of bone quality-related injuries and diseases in the

future.
FAU - Sibai, Tarek
AU - Sibai T
AD - Department of Orthopaedic Surgery, Boston University Medical Center, 720
Harrison
Avenue, Suite 808, Boston, MA 02118, USA.
FAU - Morgan, Elise F
AU - Morgan EF
FAU - Einhorn, Thomas A
AU - Einhorn TA
LA - eng
PT - Review
TA - Clin Orthop Relat Res
JT - Clinical orthopaedics and related research
JID - 0075674
RN - 0 (Adaptor Proteins, Signal Transducing)
RN - 0 (Anabolic Agents)
RN - 0 (Bone Density Conservation Agents)
RN - 0 (Bone Morphogenetic Proteins)
RN - 0 (Genetic Markers)
RN - 0 (Hydroxymethylglutaryl-CoA Reductase Inhibitors)
RN - 0 (Organometallic Compounds)
RN - 0 (Peptide Fragments)
RN - 0 (Prostaglandins)
RN - 0 (SOST protein, human)
RN - 0 (Thiophenes)
RN - 04NQ160FRU (strontium ranelate)
RN - 10T9CSU89I (Teriparatide)
RN - 67763-96-6 (Insulin-Like Growth Factor I)
RN - EC 2.7.10.2 (Focal Adhesion Kinase 2)
RN - GKK29YSF88 (parathyroid hormone (1-34)amide)
SB - AIM
SB - IM
MH - Adaptor Proteins, Signal Transducing
MH - Anabolic Agents/*pharmacology/therapeutic use
MH - Animals
MH - Bone Density Conservation Agents/administration & dosage/pharmacology
MH - Bone Morphogenetic Proteins/physiology
MH - Bone Remodeling/drug effects/*physiology
MH - Bone and Bones/*drug effects/*physiology
MH - Focal Adhesion Kinase 2/physiology
MH - Genetic Markers/physiology
MH - Humans
MH - Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use
MH - Insulin-Like Growth Factor I/therapeutic use
MH - Organometallic Compounds/pharmacology
MH - Osteoporosis/drug therapy
MH - Parathyroid Hormone/physiology
MH - Peptide Fragments/administration & dosage
MH - Prostaglandins/agonists
MH - Teriparatide/administration & dosage/analogs & derivatives
MH - Thiophenes/pharmacology
PMC - PMC3126945
EDAT- 2010/12/07 06:00
MHDA- 2011/10/01 06:00
CRDT- 2010/12/07 06:00
PHST- 2010/12/07 06:00 [entrez]
PHST- 2010/12/07 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - 1722 [pii]
AID - 10.1007/s11999-010-1722-9 [doi]
PST - ppublish
SO - Clin Orthop Relat Res. 2011 Aug;469(8):2215-24. doi: 10.1007/s11999-010-1722-
9.
PMID- 32723042
OWN - NLM
STAT- MEDLINE
DCOM- 20201230
LR - 20201230
VI - 25
IP - 3
DP - 2020 Sep
TI - Clinical Outcomes of Ready-Made J-Shaped Nail Fixation for Unstable
Metacarpal
Fractures.
PG - 276-280
LID - 10.1142/S2424835520500289 [doi]
AB - Background: The purpose of this study was to report the clinical outcomes of
ready-made J-shaped intramedullary nail fixation for unstable metacarpal
fractures.
Methods: A total of 25 unstable fractures from 24 patients were evaluated in
this
retrospective study, comprising 20 metacarpal neck and 5 metacarpal shaft
fractures.
The mean follow-up was 22 weeks. Functional outcomes were assessed based on
the
range of motion of the metacarpophalangeal joint. Radiographic outcomes were
evaluated by four projections of the postoperative plain radiographs at the
final
follow-up, and then were rated as excellent if projections at the fracture
site
showed no correction loss or angular deformity greater than 10°. Surgery time
and
complications during the treatments were recorded for each case. Results: All
25
fractures obtained bony union. The mean range of motion of the
metacarpophalangeal
joint was 78° (range, 45°-90°). Radiographic outcomes were excellent in 24
(96%) of
25 fractures. Only one fracture had correction loss. The mean surgery time
was 29
minutes (range, 14-61 minutes). Two cases had extensor tendon adhesion at the
insertion site, which was easily released when the implant was removed.
Conclusions:
This study demonstrates that intramedullary fixation with a ready-made J-
shaped nail
is a reliable treatment option for unstable metacarpal fractures.
FAU - Itadera, Eichi
AU - Itadera E
AD - Department of Orthopaedic Surgery, Japanese Red Cross Narita Hospital, Chiba,
Japan.
FAU - Okamoto, Seiji
AU - Okamoto S
AD - Department of Orthopaedic Surgery, Japanese Red Cross Narita Hospital, Chiba,
Japan.
LA - eng
PL - Singapore
TA - J Hand Surg Asian Pac Vol
JT - The journal of hand surgery Asian-Pacific volume
JID - 101688432
SB - IM
MH - Adolescent
MH - Adult
MH - Aged
MH - *Bone Nails
MH - Female
MH - Fracture Fixation, Intramedullary/*methods
MH - Fractures, Bone/*surgery
MH - Humans
MH - Male
MH - Metacarpal Bones/injuries/*surgery
MH - Metacarpophalangeal Joint/physiology
MH - Middle Aged
MH - Operative Time
MH - Range of Motion, Articular/physiology
MH - Young Adult
OTO - NOTNLM
OT - Intramedullary nail fixation
OT - Metacarpal fractures
EDAT- 2020/07/30 06:00
MHDA- 2020/12/31 06:00
CRDT- 2020/07/30 06:00
PHST- 2020/07/30 06:00 [entrez]
PHST- 2020/07/30 06:00 [pubmed]
PHST- 2020/12/31 06:00 [medline]
AID - 10.1142/S2424835520500289 [doi]
PST - ppublish
SO - J Hand Surg Asian Pac Vol. 2020 Sep;25(3):276-280. doi:
10.1142/S2424835520500289.
PMID- 32632163
OWN - NLM
LR - 20210706
IS - 2045-2322 (Linking)
VI - 10
IP - 1
DP - 2020 Jul 6
TI - The effect of Cu nanoparticle adding on to epoxy-based adhesive and adhesion
properties.
PG - 11038
LID - 10.1038/s41598-020-68162-4 [doi]
LID - 11038
AB - Epoxy-based adhesives are widely used for repairing or jointing the metal
sheets in
the industry. Because of their superior mechanical properties, the metallic
nanoparticles can be selected as the additive of the epoxy adhesive. The
strength of
the Cu nanoparticles (CuNPs) can be expected to improve the mechanical
properties of
neat epoxy. In this study, CuNPs were added at various weight ratios, such as
1, 2,
5, 10, 15, and 20% into the epoxy resin adhesive. Tensile tests of the dog-
bone
specimens and the lap-shear tensile tests of the single lap joints were
performed
for obtaining the mechanical properties. In order to investigate the failure
mechanisms, the fractured surfaces of the tensile test samples and adhesively
joined
sheets were imaged by using a Scanning Electron Microscope. The thermal
properties
of the adhesives were obtained by using Thermo Gravimetric Analysis and
Differential
Thermal Analysis. The mechanical and thermal properties of epoxy resin
adhesive were
improved by adding the CuNPs. The best adding ratios of CuNPs into epoxy were
obtained by both mechanical and thermally point of views. As a result of this

study,
15 wt% the ratio of Cu nanoparticle adding into the epoxy-based adhesive is
suitable
for improving the mechanical properties. On the other hand, 20% is the proper
Cu
nanoparticle adding ratio for the thermal properties improving.
FAU - Necati Ataberk
AU - Necati Ataberk
AUID- ORCID: 0000-0002-5394-9549
AD - Mechanical Engineering Department, Necmettin Erbakan University, Konya,
Turkey.
nataberk@erbakan.edu.tr.
LA - eng
DEP - 20200706
TA - Sci Rep
JID - 101563288
SB - IM
PMC - PMC7338459
EDAT- 2020/07/08 06:00
MHDA- 2020/07/08 06:01
CRDT- 2020/07/08 06:00
PHST- 2020/01/02 00:00 [received]
PHST- 2020/06/15 00:00 [accepted]
PHST- 2020/07/08 06:00 [entrez]
PHST- 2020/07/08 06:00 [pubmed]
PHST- 2020/07/08 06:01 [medline]
AID - 10.1038/s41598-020-68162-4 [pii]
AID - 68162 [pii]
AID - 10.1038/s41598-020-68162-4 [doi]
PST - epublish
SO - Sci Rep. 2020 Jul 6;10(1):11038. doi: 10.1038/s41598-020-68162-4.
PMID- 31811849
OWN - NLM
STAT- MEDLINE
DCOM- 20200930
LR - 20200930
IS - 0141-8130 (Linking)
VI - 143
DP - 2020 Jan 15
TI - Phosphorylation of chitosan to improve osteoinduction of chitosan/xanthan-
based
scaffolds for periosteal tissue engineering.
PG - 619-632
LID - S0141-8130(19)36980-6 [pii]
LID - 10.1016/j.ijbiomac.2019.12.004 [doi]
AB - The periosteum is a membrane that surrounds bones, providing essential
cellular and
biological components for fracture healing and bone repair. Tissue engineered
scaffolds able to function as periosteum substitutes can significantly

improve bone
regeneration in severely injured tissues. Efforts to develop more bioactive
and
tunable periosteal substitutes are required to improve the success of this
tissue
engineering approach. In this work, a chemical modification was performed in
chitosan, a polysaccharide with osteoconductive properties, by introducing
phosphate
groups to its structure. The phosphorylated polymer (Chp) was used to produce
chitosan-xanthan-based scaffolds for periosteal tissue engineering. Porous

and
mechanically reinforced matrices were obtained with addition of the
surfactant
Kolliphor® P188 and the silicone rubber Silpuran® 2130A/B. Scaffolds
properties,
such as large pore sizes (850-1097 μm), micro-roughness and thickness (0.7-
3.5 mm in
culture medium), as well as low thrombogenicity compared to standard
implantable
materials, extended degradation time and negligible cytotoxicity, enable
their
application as periosteum substitutes. Moreover, the higher adsorption of
bone
morphogenetic protein mimic (cytochrome C) by Chp-based formulations suggests
improved osteoinductivity of these materials, indicating that, when used in

vivo,
the material would be able to concentrate native BMPs and induce
osteogenesis. The
scaffolds produced were not toxic to adipose tissue-derived stem cells,
however,
cell adhesion and proliferation on the scaffolds surfaces can be still
further
improved. The mineralization observed on the surface of all formulations
indicates
that the materials studied have promising characteristics for the application
in
bone regeneration.
FAU - Bombaldi de Souza, Renata Francielle
AU - Bombaldi de Souza RF
AD - Department of Engineering of Materials and of Bioprocesses, School of
Chemical
Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil.
FAU - Bombaldi de Souza, Fernanda Carla
AU - Bombaldi de Souza FC
Chemical
FAU - Thorpe, Andrea
AU - Thorpe A
AD - Department of Mechanical Engineering, School of Biomedical Engineering,
Colorado
State University (CSU), Fort Collins, CO, USA.
FAU - Mantovani, Diego
AU - Mantovani D
AD - Laboratory for Biomaterials and Bioengineering, Canada Research Chair I in
Biomaterials and Bioengineering for the Innovation in Surgery, Department of
Min-Met-Materials Engineering, Research Center of CHU de Quebec, Division of
Regenerative Medicine, Laval University, Quebec, QC, Canada.
FAU - Popat, Ketul C
AU - Popat KC
AD - Department of Mechanical Engineering, School of Biomedical Engineering,
Colorado
State University (CSU), Fort Collins, CO, USA.
FAU - Moraes, Ângela Maria
AU - Moraes ÂM
Chemical
Electronic
address: ammoraes@feq.unicamp.br.
LA - eng
DEP - 20191204
PL - Netherlands
TA - Int J Biol Macromol
JT - International journal of biological macromolecules
JID - 7909578
RN - 0 (Polysaccharides, Bacterial)
RN - 9007-43-6 (Cytochromes c)
RN - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN - EC 3.2.1.17 (Muramidase)
RN - SY7Q814VUP (Calcium)
RN - TTV12P4NEE (xanthan gum)
SB - IM
MH - Adipose Tissue/cytology
MH - Adsorption
MH - Calcium/metabolism
MH - Cell Death/drug effects
MH - Chitosan/*pharmacology
MH - Cytochromes c/metabolism
MH - Elastic Modulus
MH - Humans
MH - L-Lactate Dehydrogenase/metabolism
MH - Muramidase/metabolism
MH - Osseointegration/*drug effects
MH - Periosteum/drug effects/*physiology
MH - Phosphorylation
MH - Polysaccharides, Bacterial/*pharmacology
MH - Porosity
MH - Stem Cells/cytology/drug effects
MH - Thrombosis/pathology
OTO - NOTNLM
OT - Chitosan
OT - Periosteum
OT - Phosphorylation
OT - Silicone
OT - Surfactant
OT - Tissue engineering
OT - Xanthan
EDAT- 2019/12/08 06:00
MHDA- 2020/10/02 06:00
CRDT- 2019/12/08 06:00
PHST- 2019/08/29 00:00 [received]
PHST- 2019/11/27 00:00 [revised]
PHST- 2019/12/01 00:00 [accepted]
PHST- 2019/12/08 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
PHST- 2019/12/08 06:00 [entrez]
AID - S0141-8130(19)36980-6 [pii]
AID - 10.1016/j.ijbiomac.2019.12.004 [doi]
PST - ppublish
SO - Int J Biol Macromol. 2020 Jan 15;143:619-632. doi:
10.1016/j.ijbiomac.2019.12.004.
Epub 2019 Dec 4.
PMID- 27655624
OWN - NLM
STAT- MEDLINE
DCOM- 20180312
LR - 20181113
IS - 1618-1247 (Linking)
VI - 105
IP - 2
DP - 2017 Apr
TI - Periodontal tissue repair after sealing of the gap in vertical root fracture.
PG - 202-207
LID - 10.1007/s10266-016-0270-5 [doi]
AB - The aim of this study was to determine whether sealing of fracture gap using
adhesive resin through the root canal can prevent inflammation of periodontal
tissue, and resealing the incompletely sealed fracture gap from outside can
resolve
such inflammation in experimentally created vertical root fractures. Vertical
root
fractures were created in incisor of beagles. In the experimental group, the
fracture gap was sealed through the root canal with adhesive resin. After
5 weeks,
sites with the clinical attachment level ≥4 mm were further divided randomly
into
the poor-replanting group and the poor-untreated group. In the poor-
replanting
group, the tooth was extracted and replanted after resealing the fracture gap
with
adhesive resin from the outer surface. Sites with clinical attachment
level ≤3 mm
after 5 weeks were considered as the satisfactory group. The poor-untreated
group
and the satisfactory group were subjected to no further treatment. The
clinical
attachment level was evaluated at baseline and after 2, 5, and 9 weeks. After
9 weeks, histological measurements were made to determine the length of the

epithelial downgrowth and the area of alveolar bone resorption. The clinical
attachment level and the area of bone resorption were significantly smaller
in the
poor-replanting group and the satisfactory group than in the poor-untreated
group
(p < 0.05). The results indicate the possibility that periodontal
inflammation along
the fracture line can be prevented and improved if the fracture gap is
sealed.
FAU - Sugaya, Tsutomu
AU - Sugaya T
AD - Department of Periodontology and Endodontology, Hokkaido University Graduate
School
of Dental Medicine, Kita 13 Nishi 7 Kita-ku, Sapporo, 060-8586, Japan.
sugaya@den.hokudai.ac.jp.
FAU - Tomita, Mahito
AU - Tomita M
School
FAU - Motoki, Youji
AU - Motoki Y
School
FAU - Zaman, Khurshiduz
AU - Zaman K
AD - Department of Dentistry, Dinajpur Medical College and Hospital, Dinajpur,
5200,
Bangladesh.
FAU - Miyaji, Hirofumi
AU - Miyaji H
School
FAU - Kawanami, Masamitsu
AU - Kawanami M
School
LA - eng
DEP - 20160921
PL - Japan
TA - Odontology
JT - Odontology
JID - 101134822
RN - 0 (Cyanoacrylates)
RN - 0 (Super Bonder Instant Adhesive)
SB - D
SB - IM
MH - Animals
MH - Cyanoacrylates/*pharmacology
MH - Dogs
MH - Incisor
MH - Male
MH - Maxilla
MH - Periodontitis/*prevention & control
MH - Random Allocation
MH - Tooth Fractures/*therapy
MH - Tooth Root/*injuries
OTO - NOTNLM
OT - Adhesive resin
OT - Attachment level
OT - Gap sealing
OT - Periodontal inflammation
OT - Vertical root fracture
EDAT- 2016/09/23 06:00
MHDA- 2018/03/13 06:00
CRDT- 2016/09/23 06:00
PHST- 2016/02/26 00:00 [received]
PHST- 2016/07/11 00:00 [accepted]
PHST- 2016/09/23 06:00 [pubmed]
PHST- 2018/03/13 06:00 [medline]
PHST- 2016/09/23 06:00 [entrez]
AID - 10.1007/s10266-016-0270-5 [pii]
AID - 10.1007/s10266-016-0270-5 [doi]
PST - ppublish
SO - Odontology. 2017 Apr;105(2):202-207. doi: 10.1007/s10266-016-0270-5. Epub
2016 Sep
21.
PMID- 29699560
OWN - NLM
STAT- MEDLINE
DCOM- 20181001
LR - 20181202
VI - 13
IP - 1
DP - 2018 Apr 27
TI - Alcohol exposure decreases osteopontin expression during fracture healing and
osteopontin-mediated mesenchymal stem cell migration in vitro.

PG - 101
LID - 10.1186/s13018-018-0800-7 [doi]
LID - 101
AB - BACKGROUND: Alcohol consumption is a risk factor for impaired fracture
healing,
though the mechanism(s) by which this occurs are not well understood. Our
laboratory
has previously shown that episodic alcohol exposure of rodents negatively
affects
fracture callus development, callus biomechanics, and cellular signaling
which
regulates stem cell differentiation. Here, we examine whether alcohol alters
chemokine expression and/or signaling activity in the mouse fracture callus
during
early fracture healing. METHODS: A mouse model for alcohol-impaired tibia
fracture
healing was utilized. Early fracture callus was examined for alcohol-effects
on
tissue composition, expression of chemokines involved in MSC migration to the
fracture site, and biomechanics. The effects of alcohol on MSC migration and
cell
adhesion receptors were examined in an in vitro system. RESULTS: Mice exposed
to
alcohol showed decreased evidence of external callus formation, decreased
callus-related osteopontin (OPN) expression levels, and decreased
biomechanical
stiffness. Alcohol exposure decreased rOPN-mediated MSC migration and
integrin β1
receptor expression in vitro. CONCLUSIONS: The effects of alcohol exposure
demonstrated here on fracture callus-associated OPN expression, rOPN-mediated
MSC
migration in vitro, and MSC integrin β1 receptor expression in vitro have not
been
previously reported. Understanding the effects of alcohol exposure on the
early
stages of fracture repair may allow timely initiation of treatment to
mitigate the
long-term complications of delayed healing and/or fracture non-union.
FAU - Natoli, Roman M
AU - Natoli RM
AD - Department of Orthopaedic Surgery and Rehabilitation, Stritch School of
Medicine,
Loyola University Chicago, 2160 South First Ave, Maywood, IL, 60153, USA.
AD - Present Address: Department of Orthopaedic Surgery, Indiana University School
of
Medicine, Indianapolis, IN, USA.
FAU - Yu, Henry
AU - Yu H
Medicine,
FAU - Meislin, Megan Conti-Mica
AU - Meislin MC
Medicine,
AD - Present Address: Department of Orthopaedic Surgery and Rehabilitation
Medicine, Hand
and Upper Extremity Division, The University of Chicago, Chicago, IL, USA.
FAU - Abbasnia, Pegah
AU - Abbasnia P
Medicine,
AD - Present Address: School of Veterinary Medicine, University of Pennsylvania,
Philadelphia, PA, USA.
FAU - Roper, Philip
AU - Roper P
Medicine,
FAU - Vuchkovska, Aleksandra
AU - Vuchkovska A
Medicine,
FAU - Xiao, Xianghui
AU - Xiao X
AD - Present Address: Argonne National Laboratory Advanced Photon Source, Lemont,
IL,
USA.
FAU - Stock, Stuart R
AU - Stock SR
AD - Present Address: School of Medicine, Northwestern University Feinberg,
Chicago, IL,
USA.
FAU - Callaci, John J
AU - Callaci JJ
Medicine,
jcallaci@luc.edu.
LA - eng
GR - AA025551/National Institute on Alcohol Abuse and Alcoholism/
GR - R21 AA025551/AA/NIAAA NIH HHS/United States
GR - 12-024/Orthopaedic Research and Education Foundation/
GR - R21 AA021225/AA/NIAAA NIH HHS/United States
GR - AA021225/National Institute on Alcohol Abuse and Alcoholism/
DEP - 20180427
TA - J Orthop Surg Res
JT - Journal of orthopaedic surgery and research
JID - 101265112
RN - 0 (Spp1 protein, mouse)
RN - 106441-73-0 (Osteopontin)
RN - 3K9958V90M (Ethanol)
SB - IM
MH - Animals
MH - Cell Movement/*drug effects/physiology
MH - Ethanol/*toxicity
MH - Fracture Healing/*drug effects/physiology
MH - Gene Expression
MH - Male
MH - Mesenchymal Stem Cells/*drug effects/metabolism
MH - Mice
MH - Osteopontin/*antagonists & inhibitors/*biosynthesis/genetics
MH - Tibia/drug effects/injuries/metabolism
PMC - PMC5921778
OTO - NOTNLM
OT - Alcohol
OT - Bone fracture
OT - Fracture non-union
OT - Integrin
OT - Mesenchymal stem cell migration
OT - Osteopontin
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Our laboratory received approval
to
conduct the animal studies described in this manuscript from the Loyola
University
Institutional Animal Care and Use Committee (IACUC). Letter of approval is on
file
with JOSR. COMPETING INTERESTS: The authors declare that they have no
competing
interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to
jurisdictional claims in published maps and institutional affiliations.
EDAT- 2018/04/28 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/04/28 06:00
PHST- 2017/10/30 00:00 [received]
PHST- 2018/04/03 00:00 [accepted]
PHST- 2018/04/28 06:00 [entrez]
PHST- 2018/04/28 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
AID - 10.1186/s13018-018-0800-7 [pii]
AID - 800 [pii]
AID - 10.1186/s13018-018-0800-7 [doi]
PST - epublish
SO - J Orthop Surg Res. 2018 Apr 27;13(1):101. doi: 10.1186/s13018-018-0800-7.
PMID- 23240664
OWN - NLM
STAT- MEDLINE
DCOM- 20130701
LR - 20121217
IS - 0001-6489 (Linking)
VI - 133
IP - 1
DP - 2013 Jan
TI - Open reduction of nasal bone fractures through an intercartilaginous
incision.
PG - 77-81
LID - 10.3109/00016489.2012.712215 [doi]
AB - CONCLUSION: Open reduction through an intercartilaginous incision was useful
for
treating delayed-diagnosed nasal bone fractures because it resulted in a
successful
outcome with minimal complications. OBJECTIVES: Nasal bone fractures are
generally
managed with closed reduction, which is usually inadequate and results in
airway
obstruction with a delayed diagnosis of nasal bone fracture when bone healing
and
fibrotic adhesions around the bone fragment have progressed. This study
investigated
the surgical outcome of open reduction through an intercartilaginous incision
for
delayed-diagnosis nasal bone fractures. METHODS: The study enrolled 18
patients who
underwent open reduction through an intercartilaginous incision to correct
delayed-diagnosis nasal bone fractures. Three independent
otorhinolaryngologists
evaluated the outcomes 4-35 months (average 12.7 months) postoperatively as
excellent, fair or poor. RESULTS: The time from injury to surgery was 11-39
days
(20-39 days in adults and 11-30 days in children). The 18 cases included 16
primary
repairs and two revisions. A Kirschner wire was inserted in six (33.3%)
patients who
had unstable reduced nasal bones. Postoperatively, l5 (83%) patients had
excellent
results, two (11%) had fair, and one (6%) had a poor outcome. No patient
experienced
any complication.
FAU - Kim, Ji Heui
AU - Kim JH
AD - Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred
Heart
Hospital, Hallym University College of Medicine, Chuncheon, South Korea.
FAU - Lee, Jun Ho
AU - Lee JH
FAU - Hong, Seok Min
AU - Hong SM
FAU - Park, Chan Hum
AU - Park CH
LA - eng
PL - England
TA - Acta Otolaryngol
JT - Acta oto-laryngologica
JID - 0370354
SB - IM
MH - Adolescent
MH - Adult
MH - Child
MH - Cohort Studies
MH - Delayed Diagnosis
MH - Female
MH - Fracture Fixation, Internal/*methods
MH - Humans
MH - Male
MH - Middle Aged
MH - Nasal Bone/*injuries
MH - Nasal Cartilages/*surgery
MH - Skull Fractures/diagnosis/etiology/*surgery
MH - Young Adult
EDAT- 2012/12/18 06:00
MHDA- 2013/07/03 06:00
CRDT- 2012/12/18 06:00
PHST- 2012/12/18 06:00 [entrez]
PHST- 2012/12/18 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
AID - 10.3109/00016489.2012.712215 [doi]
PST - ppublish
SO - Acta Otolaryngol. 2013 Jan;133(1):77-81. doi: 10.3109/00016489.2012.712215.
PMID- 30347467
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR - 20200622
IS - 0884-0431 (Print)
IS - 0884-0431 (Linking)
VI - 34
IP - 3
DP - 2019 Mar
TI - Chondrocytes Promote Vascularization in Fracture Healing Through a FOXO1-
Dependent
Mechanism.
PG - 547-556
LID - 10.1002/jbmr.3610 [doi]
AB - Chondrocytes play an essential role in fracture healing by producing
cartilage,
which forms an anlage for endochondral ossification that stabilizes the
healing
fracture callus. More recently it has been appreciated that chondrocytes have
the
capacity to produce factors that may affect the healing process. We examined
the
role of chondrocytes in angiogenesis during fracture healing and the role of
the
transcription factor forkhead box-O 1 (FOXO1), which upregulates wound
healing in
soft tissue. Closed fractures were induced in experimental mice with
lineage-specific FOXO1 deletion by Cre recombinase under the control of a
collagen-2α1 promoter element (Col2α1Cre(+) FOXO1(L/L) ) and Cre recombinase
negative control littermates containing flanking loxP sites (Col2α1Cre(-)
FOXO1(L/L)
). Experimental mice had significantly reduced CD31(+) new vessel formation.
Deletion of FOXO1 in chondrocytes in vivo suppressed the expression of
vascular
endothelial growth factor-A (VEGFA) at both the protein and mRNA levels.
Overexpression of FOXO1 in chondrocytes in vitro increased VEGFA mRNA levels
and
VEGFA transcriptional activity whereas silencing FOXO1 reduced it. Moreover,
FOXO1
interacted directly with the VEGFA promoter and a deacetylated FOXO1 mutant
enhanced
VEGFA expression whereas an acetylated FOXO1 mutant did not. Lastly, FOXO1
knockdown
by siRNA significantly reduced the capacity of chondrocytes to stimulate
microvascular endothelial cell tube formation in vitro. The results indicate
that
chondrocytes play a key role in angiogenesis which is FOXO1 dependent and
that FOXO1
in chondrocytes regulates a potent angiogenic factor, VEGFA. These studies
provide
new insight into fracture healing given the important role of vessel
formation in
the fracture repair process. © 2018 American Society for Bone and Mineral
Research.
CI - © 2018 American Society for Bone and Mineral Research.
FAU - Zhang, Citong
AU - Zhang C
AD - Department of Periodontics, School of Dental Medicine, University of
Pennsylvania,
AD - Department of Implantology, School of Stomatology, Jilin University,
Changchun,
China.
FAU - Feinberg, Daniel
AU - Feinberg D
Pennsylvania,
FAU - Alharbi, Mohammed
AU - Alharbi M
Pennsylvania,
AD - Department of Endodontics, Faculty of Dentistry, King Abdulaziz University,
Jeddah,
KSA.
FAU - Ding, Zhenjiang
AU - Ding Z
Pennsylvania,
AD - Department of Pediatric Dentistry, School of Stomatology, China Medical
University,
Shenyang, China.
AD - Key Laboratory of Oral Disease and Liaoning Province, Shenyang, China.
FAU - Lu, Chanyi
AU - Lu C
Pennsylvania,
AU - O'Connor JP
AD - Department of Orthopaedics, New Jersey Medical School, Rutgers, The State
University
of New Jersey, Newark, NJ, USA.
FAU - Graves, Dana T
AU - Graves DT
Pennsylvania,
LA - eng
DEP - 20181120
TA - J Bone Miner Res
JT - Journal of bone and mineral research : the official journal of the American
Society
for Bone and Mineral Research
JID - 8610640
RN - 0 (Col2a1 protein, mouse)
RN - 0 (Collagen Type II)
RN - 0 (Forkhead Box Protein O1)
RN - 0 (Foxo1 protein, mouse)
RN - 0 (Pecam1 protein, mouse)
RN - 0 (Vascular Endothelial Growth Factor A)
RN - 0 (vascular endothelial growth factor A, mouse)
SB - IM
MH - Animals
MH - Cell Line
MH - Chondrocytes/*metabolism
MH - Collagen Type II/biosynthesis/genetics
MH - Down-Regulation
MH - Endothelial Cells/pathology
MH - Forkhead Box Protein O1/genetics/*metabolism
MH - Gene Deletion
MH - Mice
MH - Mice, Transgenic
MH - *Neovascularization, Physiologic
MH - Platelet Endothelial Cell Adhesion Molecule-1/biosynthesis
MH - Transcription, Genetic
MH - Vascular Endothelial Growth Factor A/*biosynthesis/genetics
PMC - PMC6414243
MID - NIHMS1004521
OTO - NOTNLM
OT - *BONE
OT - *CHONDROCYTE AND CARTILAGE BIOLOGY
OT - *ChIP
OT - *FRACTURE HEALING
OT - *GENETIC ANIMAL MODELS
COIS- Disclosures The authors declare no conflicts of interest.
EDAT- 2018/10/23 06:00
MHDA- 2020/06/23 06:00
CRDT- 2018/10/23 06:00
PHST- 2018/02/27 00:00 [received]
PHST- 2018/10/08 00:00 [revised]
PHST- 2018/10/11 00:00 [accepted]
PHST- 2018/10/23 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
PHST- 2018/10/23 06:00 [entrez]
AID - 10.1002/jbmr.3610 [doi]
PST - ppublish
SO - J Bone Miner Res. 2019 Mar;34(3):547-556. doi: 10.1002/jbmr.3610. Epub 2018
Nov 20.
PMID- 26763079
OWN - NLM
STAT- MEDLINE
DCOM- 20171221
LR - 20180829
IS - 0884-0431 (Linking)
VI - 31
IP - 6
DP - 2016 Jun
TI - Neurotrophin-3 Induces BMP-2 and VEGF Activities and Promotes the Bony Repair
of
Injured Growth Plate Cartilage and Bone in Rats.
PG - 1258-74
LID - 10.1002/jbmr.2786 [doi]
AB - Injured growth plate is often repaired by bony tissue causing bone growth
defects,
for which the mechanisms remain unclear. Because neurotrophins have been
implicated
in bone fracture repair, here we investigated their potential roles in growth
plate
bony repair in rats. After a drill-hole injury was made in the tibial growth
plate
and bone, increased injury site mRNA expression was observed for
neurotrophins NGF,
BDNF, NT-3, and NT-4 and their Trk receptors. NT-3 and its receptor TrkC
showed the
highest induction. NT-3 was localized to repairing cells, whereas TrkC was
observed
in stromal cells, osteoblasts, and blood vessel cells at the injury site.
Moreover,
systemic NT-3 immunoneutralization reduced bone volume at injury sites and
also
reduced vascularization at the injured growth plate, whereas recombinant NT-3
treatment promoted bony repair with elevated levels of mRNA for osteogenic
markers
and bone morphogenetic protein (BMP-2) and increased vascularization and mRNA
for
vascular endothelial growth factor (VEGF) and endothelial cell marker CD31 at
the
injured growth plate. When examined in vitro, NT-3 promoted osteogenesis in
rat bone
marrow stromal cells, induced Erk1/2 and Akt phosphorylation, and enhanced
expression of BMPs (particularly BMP-2) and VEGF in the mineralizing cells.
It also
induced CD31 and VEGF mRNA in rat primary endothelial cell culture. BMP
activity
appears critical for NT-3 osteogenic effect in vitro because it can be almost
completely abrogated by co-addition of the BMP inhibitor noggin. Consistent

with its
angiogenic effect in vivo, NT-3 promoted angiogenesis in metatarsal bone
explants,
an effect abolished by co-treatment with anti-VEGF. This study suggests that
NT-3
may be an osteogenic and angiogenic factor upstream of BMP-2 and VEGF in bony
repair, and further studies are required to investigate whether NT-3 may be a
potential target for preventing growth plate faulty bony repair or for
promoting
bone fracture healing. © 2016 American Society for Bone and Mineral Research.
FAU - Su, Yu-Wen
AU - Su YW
AD - School of Pharmacy and Medical Sciences, Sansom Institute for Health
Research,
University of South Australia, Adelaide, Australia.
FAU - Chung, Rosa
AU - Chung R
Research,
FAU - Ruan, Chun-Sheng
AU - Ruan CS
Research,
FAU - Chim, Shek Man
AU - Chim SM
AD - School of Pathology and Laboratory Medicine, University of Western Australia,
Nedlands, Australia.
FAU - Kuek, Vincent
AU - Kuek V
FAU - Dwivedi, Prem P
AU - Dwivedi PP
Research,
FAU - Hassanshahi, Mohammadhossein
AU - Hassanshahi M
Research,
FAU - Chen, Ke-Ming
AU - Chen KM
AD - Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou Command of CPLA,
Lanzhou, China.
FAU - Xie, Yangli
AU - Xie Y
AD - Center of Bone Metabolism and Repair, State Key Laboratory of Trauma, Burns,
and
Combined Injury, Daping Hospital, Third Military Medical University,
Chongqing,
China.
FAU - Chen, Lin
AU - Chen L
AD - Center of Bone Metabolism and Repair, State Key Laboratory of Trauma, Burns,
and
Combined Injury, Daping Hospital, Third Military Medical University,
Chongqing,
China.
FAU - Foster, Bruce K
AU - Foster BK
AD - Department of Orthopaedic Surgery, Women's and Children's Hospital, North
Adelaide,
Australia.
FAU - Rosen, Vicki
AU - Rosen V
AD - Department of Developmental Biology, Harvard School of Dental Medicine,
Boston, MA,
USA.
FAU - Zhou, Xin-Fu
AU - Zhou XF
Research,
FAU - Xu, Jiake
AU - Xu J
FAU - Xian, Cory J
AU - Xian CJ
Research,
LA - eng
DEP - 20160216
PL - United States
Society
JID - 8610640
RN - 0 (Neurotrophin 3)
RN - 0 (vascular endothelial growth factor A, rat)
RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB - IM
MH - Animals
MH - Bone Morphogenetic Protein 2/*metabolism
MH - Cartilage/*metabolism
MH - Growth Plate/*metabolism
MH - MAP Kinase Signaling System/physiology
MH - Male
MH - Mitogen-Activated Protein Kinase 3/metabolism
MH - Neurotrophin 3/*metabolism
MH - Proto-Oncogene Proteins c-akt/metabolism
MH - Rats
MH - Rats, Sprague-Dawley
MH - Vascular Endothelial Growth Factor A/*metabolism
OTO - NOTNLM
OT - *BMP-2
OT - *BONE REPAIR
OT - *GROWTH PLATE
OT - *NEUROTROPHIC FACTOR
OT - *NEUROTROPHIN-3
OT - *VEGF
EDAT- 2016/01/15 06:00
MHDA- 2017/12/22 06:00
CRDT- 2016/01/15 06:00
PHST- 2015/04/02 00:00 [received]
PHST- 2016/01/06 00:00 [revised]
PHST- 2016/01/08 00:00 [accepted]
PHST- 2016/01/15 06:00 [entrez]
PHST- 2016/01/15 06:00 [pubmed]
PHST- 2017/12/22 06:00 [medline]
AID - 10.1002/jbmr.2786 [doi]
PST - ppublish
SO - J Bone Miner Res. 2016 Jun;31(6):1258-74. doi: 10.1002/jbmr.2786. Epub 2016
Feb 16.
PMID- 30539752
OWN - NLM
STAT- MEDLINE
DCOM- 20190523
LR - 20190523
IS - 0020-1383 (Linking)
VI - 49
IP - 12
DP - 2018 Dec
TI - Utility of early active motion for flexor tendon repair with concomitant
injuries: A
multivariate analysis.
PG - 2248-2251
LID - S0020-1383(18)30627-2 [pii]
LID - 10.1016/j.injury.2018.10.022 [doi]
AB - INTRODUCTION: Flexor tendon injury often occurs with concomitant injuries
such as
fracture, vascular injury, and extensor tendon injury. These injuries are
repaired
independently, without a comprehensive strategy. We aimed to identify the
effect of
concomitant injuries and treatment choice on the outcome of flexor tendon
repair.
PATIENTS AND METHODS: We evaluated 118 fingers of 102 adult patients with
zone 1-3
flexor digitorum profundus (FDP) tendon injuries who underwent primary
surgery at
our hospital between April 2009 and December 2017. The 2-strand pull-out, 4-
strand
Tsuge, 6-strand Lim & Tsai, and 8-strand cross-locked cruciate suturing
techniques
were used. We performed multivariate analyses, with the active range of
motion
(AROM) of the proximal interphalangeal (PIP) and distal interphalangeal (DIP)
joints
as dependent variables, and age, existence of concomitant injuries, and their
treatment as independent variables. RESULTS: The average AROM of the PIP +

DIP
joints was 130° at the last follow-up, and 'excellent' or 'good' function was
obtained in 74 (63%) of 118 fingers by using the Strickland criteria. Old

age,
concomitant diaphyseal fractures, and specific methods of osteosynthesis,
such as
pinning, flexor digitorum superficialis injury, and immobilisation for 3
weeks,
significantly worsened the results. However, wiring for osteosynthesis and
early
active motion protocol improved postoperative functional outcome. Although
the
outcome did not differ among the suture techniques, the 4-strand Tsuge
procedure was
performed for the two surgically confirmed ruptures of repair that occurred
in our
series. DISCUSSION: We clarified the superiority of early mobilisation
protocols
with rigid osteosynthesis procedure, other than pinning. To minimise tendon
adhesion
or joint stiffness, surgeons should repair the tendon and fractured bone
appropriately, to ensure early mobilisation without serious complications.
FAU - Fujihara, Yuki
AU - Fujihara Y
AD - Department of Orthopaedic Surgery, Nagoya Ekisaikai Hospital, 4-66 Shonen-
Cho,
Nakagawa-Ku, Nagoya, 454-8502, Japan. Electronic address:
yukifujihara@yahoo.co.jp.
FAU - Ota, Hideyuki
AU - Ota H
Cho,
Nakagawa-Ku, Nagoya, 454-8502, Japan.
FAU - Watanabe, Kentaro
AU - Watanabe K
Cho,
Nakagawa-Ku, Nagoya, 454-8502, Japan.
LA - eng
PT - Observational Study
DEP - 20181022
PL - Netherlands
TA - Injury
JT - Injury
JID - 0226040
SB - IM
MH - Adult
MH - *Early Ambulation
MH - Female
MH - Finger Injuries/physiopathology/rehabilitation/*surgery
MH - Fractures, Bone/physiopathology/rehabilitation/*surgery
MH - Humans
MH - Male
MH - Middle Aged
MH - Range of Motion, Articular/*physiology
MH - Recovery of Function/physiology
MH - Tendon Injuries/physiopathology/rehabilitation/*surgery
MH - Vascular System Injuries/physiopathology/rehabilitation/*surgery
MH - Young Adult
OTO - NOTNLM
OT - Complex injury
OT - Early active motion
OT - Flexor tendon injury
OT - Fracture fixation
OT - Prognostic factor
OT - Tendon repair
EDAT- 2018/12/13 06:00
MHDA- 2019/05/24 06:00
CRDT- 2018/12/13 06:00
PHST- 2018/05/09 00:00 [received]
PHST- 2018/10/06 00:00 [revised]
PHST- 2018/10/19 00:00 [accepted]
PHST- 2018/12/13 06:00 [entrez]
PHST- 2018/12/13 06:00 [pubmed]
PHST- 2019/05/24 06:00 [medline]
AID - S0020-1383(18)30627-2 [pii]
AID - 10.1016/j.injury.2018.10.022 [doi]
PST - ppublish
SO - Injury. 2018 Dec;49(12):2248-2251. doi: 10.1016/j.injury.2018.10.022. Epub
2018 Oct
22.
PMID- 30829593
OWN - NLM
STAT- MEDLINE
DCOM- 20190830
LR - 20190830
IS - 1512-0112 (Print)
IS - 1512-0112 (Linking)
IP - 286
DP - 2019 Jan
TI - STUDY OF BONE TISSUE REPARATION AFTER A FEMUR FRACTURE DEPENDING ON THE
CORRECTION
OF ARTERIAL HYPERTENSION IN MODEL OBJECT RATTUS NORVEGICUS (RAT GRAY).
PG - 72-77
AB - The processes of bone remodeling and regulation of blood pressure are caused
by
common genetic determinants underlying the development of these diseases. To
solve
practical medical problems, it seems relevant to carry out the correction and
treatment of syntropic diseases at the same time with the aim of achieving
the
maximum positive effect. The most serious complication of osteoporosis is
bone
fractures, especially fractures of the proximal femur, which are a serious
medical
and social problem. The aim of our work was to evaluate the repair of bone
tissue
after fractures of the proximal femur with subsequent intramedullary
osteosynthesis
on the background of arterial hypertension and its correction. A model object
was
used in the work - gray rats, lines Wistar and SHR, which were operated on.
For the
animals of one of the groups with genetically determined arterial
hypertension,
correction of the above-mentioned pathology in the form of enalapril
monotherapy in
the postoperative period was applied. In this group, the picture of the
dynamics of
blood pressure indices corresponded to the cumulative effect of enalapril -
after
three weeks of taking the drug, the rats' systolic blood pressure indices
corresponded to those of normotensive animals, and the indicators of adhesion
of the
fracture zone were higher than in the group of rats with arterial
hypertension,
which was not corrected. Adequate correction of the level of blood pressure
in the
postoperative period favorably affects the reparative capabilities of the
bone,
while at the same time increasing the chances of positive results of surgical
treatment of patients.
FAU - Babalyan, V
AU - Babalyan V
AD - Kharkiv Medical Academy Of Postgraduate Education; National Pharmaceutical
University; V.N. Karazin Kharkiv National University, Ukraine.
FAU - Valilshchykov, M
AU - Valilshchykov M
FAU - Pavlov, S
AU - Pavlov S
FAU - Koshevaya, E
AU - Koshevaya E
FAU - Fedota, O
AU - Fedota O
LA - eng
PL - Georgia (Republic)
TA - Georgian Med News
JT - Georgian medical news
JID - 101218222
SB - IM
MH - Animals
MH - Blood Pressure
MH - *Femoral Fractures/therapy
MH - *Hypertension
MH - Rats
MH - Rats, Inbred SHR
MH - Rats, Wistar
EDAT- 2019/03/05 06:00
MHDA- 2019/08/31 06:00
CRDT- 2019/03/05 06:00
PHST- 2019/03/05 06:00 [entrez]
PHST- 2019/03/05 06:00 [pubmed]
PHST- 2019/08/31 06:00 [medline]
PST - ppublish
SO - Georgian Med News. 2019 Jan;(286):72-77.
PMID- 25190762
OWN - NLM
STAT- MEDLINE
DCOM- 20150413
LR - 20140905
IS - 0007-1420 (Linking)
VI - 111
IP - 1
DP - 2014 Sep
TI - Myeloma bone disease: pathogenesis, current treatments and future targets.
PG - 117-38
LID - 10.1093/bmb/ldu016 [doi]
AB - INTRODUCTION: Patients with myeloma develop localized and generalized bone
loss
leading to hypercalcaemia, accelerated osteoporosis, vertebral wedge
fractures,
other pathological fractures, spinal cord compression and bone pain. Bone
loss is
mediated by a variety of biological modifiers including osteoclast-activating
factors (OAF) and osteoblast (OB) inhibitory factors produced either directly
by
malignant plasma cells (MPCs) or as a consequence of their interaction with
the bone
marrow microenvironment (BMM). Raised levels of OAFs such as receptor
activator of
nuclear factor-kappa B ligand (RANKL), macrophage inflammatory protein 1
alpha,
tumour necrosis factor-alpha and interleukin 6 stimulate bone resorption by
recruiting additional osteoclasts. Via opposing mechanisms, increases in OB
inhibitory factors, such as dickkopf-1 (Dkk-1), soluble frizzled-related
protein-3
and hepatocyte growth factor (HGF), suppress bone formation by inhibiting the
differentiation and recruitment of OBs. These changes result in an uncoupling

of
physiological bone remodelling, leading to myeloma bone disease (MBD).
Moreover, the
altered BMM provides a fertile ground for the growth and survival of MPCs.
Current
clinical management of MBD is both reactive (to pain and fractures) and
preventive,
with bisphosphonates (BPs) being the mainstay of pharmacological treatment.
However,
side effects and uncertainties associated with BPs warrant the search for
more
targeted treatments for MBD. This review will summarize recent developments
in
understanding the intimate relationship between MBD and the BMM and the novel
ways
in which they are being therapeutically targeted. SOURCES OF DATA: All data
included
were sourced and referenced from PubMed. AREAS OF AGREEMENT: The clinical
utility of
BP therapy is well established. However, there is general acknowledgement
that BPs
are only partially successful in the treatment of MBD. The number of skeletal
events
attributable to myeloma are reduced by BPs but not totally eliminated.
Furthermore,
existing damage is not repaired. It is widely recognized that more effective
treatments are needed. AREAS OF CONTROVERSY: There remains controversy
concerning
the duration of BP therapy. Whether denosumab is a viable alternative to BP
therapy
is also contested. Many of the new therapeutic strategies discussed are yet
to
translate to clinical practice and demonstrate equal efficacy or superiority
to BP
therapy. It also remains controversial whether reported anti-tumour effects
of
bone-modulating therapies are clinically significant. GROWING POINTS: The
potential
clinical utility of bone anabolic therapies including agents such as anti-
Dkk-1,
anti-sclerostin and anti-HGF is becoming increasingly recognized. AREAS
TIMELY FOR
DEVELOPING RESEARCH: Further research effectively targeting the mediators of
MBD,
targeting both bone resorption and bone formation, is urgently needed. This
should
translate promptly to clinical trials of combination therapy comprising
anti-resorptives and bone anabolic therapies to demonstrate efficacy and
improved
outcomes over BPs.
CI - © The Author 2014. Published by Oxford University Press. All rights reserved.
For
permissions, please e-mail: journals.permissions@oup.com.
FAU - Walker, Rebecca E
AU - Walker RE
AD - Sheffield Myeloma Research Team (SmaRT), Department of Oncology, University
of
Sheffield, Sheffield, UK Department of Haematology, Sheffield Teaching
Hospitals NHS
Foundation Trust, Sheffield, UK.
FAU - Lawson, Michelle A
AU - Lawson MA
of
Hospitals NHS
FAU - Buckle, Clive H
AU - Buckle CH
of
Hospitals NHS
FAU - Snowden, John A
AU - Snowden JA
of
Hospitals NHS
FAU - Chantry, Andrew D
AU - Chantry AD
of
Hospitals NHS
Foundation Trust, Sheffield, UK a.d.chantry@sheffield.ac.uk.
LA - eng
PT - Review
PL - England
TA - Br Med Bull
JT - British medical bulletin
JID - 0376542
RN - 0 (Immunologic Factors)
RN - 0 (cell aggregation factors)
SB - IM
MH - Bone Density Conservation Agents/therapeutic use
MH - Bone Diseases/*etiology/therapy
MH - Cell Adhesion Molecules/physiology
MH - Humans
MH - Immunologic Factors/therapeutic use
MH - Multiple Myeloma/*complications/therapy
MH - Osteoblasts/physiology
MH - Osteoclasts/physiology
MH - Osteoporosis/etiology/therapy
OTO - NOTNLM
OT - bone disease
OT - myeloma
OT - therapeutic targets
EDAT- 2014/09/06 06:00
MHDA- 2015/04/14 06:00
CRDT- 2014/09/06 06:00
PHST- 2014/09/06 06:00 [entrez]
PHST- 2014/09/06 06:00 [pubmed]
PHST- 2015/04/14 06:00 [medline]
AID - ldu016 [pii]
AID - 10.1093/bmb/ldu016 [doi]
PST - ppublish
SO - Br Med Bull. 2014 Sep;111(1):117-38. doi: 10.1093/bmb/ldu016.
PMID- 26664114
OWN - NLM
STAT- MEDLINE
DCOM- 20160905
LR - 20181202
IS - 1176-9114 (Print)
IS - 1176-9114 (Linking)
VI - 10
DP - 2015
TI - Combination of calcium sulfate and simvastatin-controlled release
microspheres
enhances bone repair in critical-sized rat calvarial bone defects.
PG - 7231-40
LID - 10.2147/IJN.S88134 [doi]
AB - Most allogenic bone graft substitutes have only osteoconductive properties.
Developing new strategies to improve the osteoinductive activity of bone
graft
substitutes is both critical and practical for clinical application.
Previously, we
developed novel simvastatin-encapsulating poly(lactic-co-glycolic acid)
microspheres
(SIM/PLGA) that slowly release simvastatin and enhance fracture healing. In
this
study, we combined SIM/PLGA with a rapidly absorbable calcium sulfate (CS)
bone
substitute and studied the effect on bone healing in critical-sized calvarial
bone
defects in a rat model. The cytotoxicity and cytocompatibility of this
combination
was tested in vitro using lactate dehydrogenase leakage and a cell attachment
assay,
respectively. Combination treatment with SIM/PLGA and the CS bone substitute
had no
cytotoxic effect on bone marrow stem cells. Compared with the control, cell
adhesion
was substantially enhanced following combination treatment with SIM/PLGA and
the CS
bone substitute. In vivo, implantation of the combination bone substitute
promoted
healing of critical-sized calvarial bone defects in rats; furthermore,
production of
bone morphogenetic protein-2 and neovascularization were enhanced in the area
of the
defect. In summary, the combination of SIM/PLGA and a CS bone substitute has
osteoconductive and osteoinductive properties, indicating that it could be
used for
regeneration of bone in the clinical setting.
FAU - Fu, Yin-Chih
AU - Fu YC
AD - Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung,
Taiwan ;
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung,
Taiwan ;
Department of Orthopaedics, Kaohsiung Medical University, Kaohsiung, Taiwan ;
Department of Orthopaedics, College of Medicine, Kaohsiung Medical

University,
Kaohsiung, Taiwan.
FAU - Wang, Yan-Hsiung
AU - Wang YH
Taiwan ;
School of Dentistry, College of Dental Medicine, Kaohsiung Medical
University,
Kaohsiung, Taiwan.
FAU - Chen, Chung-Hwan
AU - Chen CH
Taiwan ;

University,
Kaohsiung, Taiwan.
FAU - Wang, Chih-Kuang
AU - Wang CK
Taiwan ;
Department of Medicinal and Applied Chemistry, Kaohsiung Medical University,
Kaohsiung, Taiwan.
FAU - Wang, Gwo-Jaw
AU - Wang GJ
Taiwan ;

University,
Kaohsiung, Taiwan.
FAU - Ho, Mei-Ling
AU - Ho ML
Taiwan ;
Department of Physiology, College of Medicine, Kaohsiung Medical University,

Kaohsiung, Taiwan ; Department of Marine Biotechnology and Resources,
National Sun
Yat-sen University, Kaohsiung, Taiwan.
LA - eng
DEP - 20151201
TA - Int J Nanomedicine
JT - International journal of nanomedicine
JID - 101263847
RN - 0 (Delayed-Action Preparations)
RN - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN - 26009-03-0 (Polyglycolic Acid)
RN - 33X04XA5AT (Lactic Acid)
RN - AGG2FN16EV (Simvastatin)
RN - WAT0DDB505 (Calcium Sulfate)
SB - IM
MH - Animals
MH - Bone Regeneration/drug effects
MH - Bone Substitutes/pharmacology
MH - Calcium Sulfate/*pharmacology
MH - Delayed-Action Preparations/pharmacology
MH - Immunohistochemistry
MH - Lactic Acid/chemistry
MH - Male
MH - Mice
MH - *Microspheres
MH - Polyglycolic Acid/chemistry
MH - Polylactic Acid-Polyglycolic Acid Copolymer
MH - Simvastatin/*pharmacology
MH - Skull/drug effects/*pathology
MH - Staining and Labeling
MH - Wound Healing/*drug effects
PMC - PMC4671780
OTO - NOTNLM
OT - calcium sulfate
OT - calvarial bone defects
OT - osteoconductive
OT - osteoinductive
OT - simvastatin
EDAT- 2015/12/15 06:00
MHDA- 2016/09/07 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2016/09/07 06:00 [medline]
AID - ijn-10-7231 [pii]
AID - 10.2147/IJN.S88134 [doi]
PST - epublish
SO - Int J Nanomedicine. 2015 Dec 1;10:7231-40. doi: 10.2147/IJN.S88134.
eCollection
2015.
PMID- 25886640
OWN - NLM
STAT- MEDLINE
DCOM- 20160422
LR - 20161125
IS - 1748-6041 (Linking)
VI - 10
IP - 2
DP - 2015 Apr 17
TI - Bio-functionalized MWCNT/hyperbranched polyurethane bionanocomposite for bone
regeneration.
PG - 025011
LID - 10.1088/1748-6041/10/2/025011 [doi]
AB - The proper fabrication of biomaterials, particularly for purposes like bone
regeneration, is of the utmost importance for the clinical success of
materials that
fulfill the design criteria at bio-interfacial milieu. Building on this
aspect, a
polyurethane nanocomposite (PNC) was fabricated by the combination of
rapeseed
protein functionalized multi-walled carbon nanotubes (MWCNTs) and
vegetable-oil-based hyperbranched polyurethane. Biofunctionalized MWCNTs
showed
incredible biocompatibility compared to pristine MWCNTs as ascertained via in
vitro
and in vivo studies. PNC showed enhanced MG63 cell differentiation ability
compared
to the control and carboxyl functionalized MWCNT-based nanocomposite, as
postulated
by alkaline phosphatase activity together with better cellular adhesion,
spreading
and proliferation. Consequently, a critical-sized fracture gap (6 mm) bridged
by the
sticky PNC scaffold illustrated rapid bone neoformation within 30-45 d, with
90-93%
of the defect area filling up. Histopathological studies demonstrated the
reorganization of the normal tibial architecture and biodegradation of the
implant.
The subsequent toxicological study through cytokine expression, biochemical
analysis
and hematological studies suggested non-immunogenic and non-toxic effects of
PNCs
and their degraded/leached products. Their excellent bio-physiological
features with
high load-bearing ability (49-55.5 Mpa), ductility (675-790%) and
biodegradability
promote them as the best alternative biomaterials for bone regeneration in a
comprehensive manner.
FAU - Das, Beauty
AU - Das B
AD - Advanced Polymer and Nanomaterial Laboratory, Department of Chemical
Sciences,
Tezpur University, Tezpur-784028, India.
FAU - Chattopadhyay, Pronobesh
AU - Chattopadhyay P
FAU - Maji, Somnath
AU - Maji S
FAU - Upadhyay, Aadesh
AU - Upadhyay A
FAU - Das Purkayastha, Manashi
AU - Das Purkayastha M
FAU - Mohanta, Charu Lata
AU - Mohanta CL
FAU - Maity, Tapas Kumar
AU - Maity TK
FAU - Karak, Niranjan
AU - Karak N
LA - eng
DEP - 20150417
PL - England
TA - Biomed Mater
JID - 101285195
RN - 0 (Nanotubes, Carbon)
RN - 0 (Polyurethanes)
SB - IM
MH - Absorbable Implants
MH - Animals
MH - Biocompatible Materials/chemistry
MH - Cell Line
MH - Male
MH - Nanocomposites/*chemistry/ultrastructure
MH - Nanotubes, Carbon/*chemistry/ultrastructure
MH - Osteoblasts/cytology
MH - Polyurethanes/*chemistry
MH - Radiography
MH - Rats
MH - Rats, Wistar
MH - Tibial Fractures/diagnostic imaging/pathology/surgery
MH - Tissue Scaffolds/chemistry
EDAT- 2015/04/18 06:00
MHDA- 2016/04/23 06:00
CRDT- 2015/04/18 06:00
PHST- 2015/04/18 06:00 [entrez]
PHST- 2015/04/18 06:00 [pubmed]
PHST- 2016/04/23 06:00 [medline]
AID - 10.1088/1748-6041/10/2/025011 [doi]
PST - epublish
SO - Biomed Mater. 2015 Apr 17;10(2):025011. doi: 10.1088/1748-6041/10/2/025011.
PMID- 23899020
OWN - NLM
STAT- MEDLINE
DCOM- 20150518
LR - 20161125
IS - 2169-1401 (Linking)
VI - 42
IP - 5
DP - 2014 Oct
TI - The modification experimental study in vivo of nano-bone gelatin.
PG - 309-15
LID - 10.3109/21691401.2013.821411 [doi]
AB - OBJECTIVE: To study the feasibility of therapy using nano-bone gelatin to
comminuted
fracture by animal experiment. METHODS: The animal models of transverse
fracture
were made on bilateral ulnas of 45 New Zealand white rabbits, which were
divided
into experimental group (repair with nano-bone gelatin), control group
(repair with
traditional medical glue), and blank group (unrepaired) randomly. The
reconstruction
effect in each group was evaluated using X-ray examination, MicroCT scanning,
histopathology observation, and biomechanical test postoperation regularly.

RESULT:
On 12th week, the fractures in experimental and blank group get the marrow
cavity
completely unobstructed and good bone union; however, in control group, the
fracture
line slightly blurred with the marrow cavity not fully unobstructed; 6 weeks
later,
observation of bony calluses through MircoCT: experimental group, 68.5 ±
2.71%;
blank group, 69.19 ± 2.3%; and control group, 49.35 ± 3.56%, there were no
significant difference between the two groups (P > 0.05). The control group
obviously showed worse bone union than the former two (P < 0.05). The
histopathological examination shows that the bony calluses of experimental
group are
similar to those of the blank group; however, gelatin degraded slowly in
control
group with delayed union; on the 12th week, biomechanical test shows that the
blank
and experiment groups had basically same average bending strength values
which had
no significant difference (P > 0.05) and obviously were higher than those of
the
control group (P < 0.01). CONCLUSION: The nano-bone gelatin won't lead to
delayed
union of fractures and may be beneficial to it, and so may be an ideal
gelatin for
fixing small fractures.
FAU - Li, Wencui
AU - Li W
AD - Anhui Medical College , Anhui P. R. China.
FAU - Zhao, Zhe
AU - Zhao Z
FAU - Xiong, Jianyi
AU - Xiong J
FAU - Zeng, Yanjun
AU - Zeng Y
LA - eng
DEP - 20130730
PL - England
TA - Artif Cells Nanomed Biotechnol
JT - Artificial cells, nanomedicine, and biotechnology
JID - 101594777
RN - 0 (Adhesives)
RN - 9000-70-8 (Gelatin)
SB - IM
MH - Adhesives/*chemistry/*pharmacology
MH - Animals
MH - Biomechanical Phenomena/drug effects
MH - Fractures, Bone/diagnostic imaging/pathology
MH - Gelatin/*chemistry/*pharmacology
MH - Male
MH - *Nanostructures
MH - Organ Size/drug effects
MH - Rabbits
MH - Ulna/cytology/diagnostic imaging/*drug effects/pathology
OTO - NOTNLM
OT - cyanoacrylate
OT - fractures
OT - gelatin
OT - nanometer
EDAT- 2013/08/01 06:00
MHDA- 2015/05/20 06:00
CRDT- 2013/08/01 06:00
PHST- 2013/08/01 06:00 [entrez]
PHST- 2013/08/01 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
AID - 10.3109/21691401.2013.821411 [doi]
PST - ppublish
SO - Artif Cells Nanomed Biotechnol. 2014 Oct;42(5):309-15. doi:
10.3109/21691401.2013.821411. Epub 2013 Jul 30.
PMID- 30387212
OWN - NLM
STAT- MEDLINE
DCOM- 20190604
LR - 20200930
IS - 0935-9648 (Linking)
VI - 30
IP - 52
DP - 2018 Dec
TI - The Dawn of Thiol-Yne Triazine Triones Thermosets as a New Material Platform
Suited
for Hard Tissue Repair.
PG - e1804966
LID - 10.1002/adma.201804966 [doi]
AB - The identification of a unique set of advanced materials that can bear
extraordinary
loads for use in bone and tooth repair will inevitably unlock unlimited
opportunities for clinical use. Herein, the design of high-performance
thermosets is
reported based on triazine-trione (TATO) monomers using light-initiated
thiol-yne
coupling (TYC) chemistry as a polymerization strategy. In comparison to
traditional
thiol-ene coupling (TEC) systems, TYC chemistry has yielded highly dense
networks
with unprecedented mechanical properties. The most promising system notes 4.6
GPa in
flexural modulus and 160 MPa in flexural strength, an increase of 84% in
modulus and
191% in strength when compared to the corresponding TATO system based on TEC
chemistry. Remarkably, the mechanical properties exceed those of polylactide
(PLA)
and challenge poly(ether ether ketone) PEEK and today's methacrylate-based
dental
resin composites. All the materials display excellent biocompatibility, in
vitro,
and are successfully: i) molded into medical devices for fracture repair, and
ii)
used as bone adhesive for fracture fixation and as tooth fillers with the
outstanding bond strength that outperform methacrylate systems used today in
dental
restoration application. Collectively, a new era of advanced TYC materials is
unfolded that can fulfill the preconditions as bone fixating implants and for
tooth
restorations.
FAU - Arseneault, Mathieu
AU - Arseneault M
AUID- ORCID: 0000-0003-2118-6687
AD - KTH Royal Institute of Technology, Department of Fibre and Polymer
Technology,
SE-100 44, Stockholm, Sweden.
FAU - Granskog, Viktor
AU - Granskog V
AUID- ORCID: 0000-0001-8595-0037
Technology,
FAU - Khosravi, Sara
AU - Khosravi S
Technology,
FAU - Heckler, Ilona Maria
AU - Heckler IM
Technology,
FAU - Mesa-Antunez, Pablo
AU - Mesa-Antunez P
AUID- ORCID: 0000-0002-6990-496X
Technology,
FAU - Hult, Daniel
AU - Hult D
AUID- ORCID: 0000-0001-7543-5322
Technology,
FAU - Zhang, Yuning
AU - Zhang Y
AUID- ORCID: 0000-0002-9597-9578
Technology,
FAU - Malkoch, Michael
AU - Malkoch M
AUID- ORCID: 0000-0002-9200-8004
Technology,
LA - eng
GR - 2012-0196/Knut och Alice Wallenbergs Stiftelse/
DEP - 20181102
PL - Germany
TA - Adv Mater
JID - 9885358
RN - 0 (Triazines)
SB - IM
MH - Animals
MH - Bone and Bones/chemistry
MH - Calorimetry, Differential Scanning
MH - Cell Line
MH - Elasticity
MH - Humans
MH - Hydrogen Bonding
MH - Light-Curing of Dental Adhesives
MH - Methacrylates/chemistry
MH - Spectrum Analysis, Raman
MH - Swine
MH - Triazines/*chemistry
OTO - NOTNLM
OT - biomedical applications
OT - dental fillers
OT - implants
OT - polymeric materials
OT - thiol-yne photochemistry
EDAT- 2018/11/06 06:00
MHDA- 2019/06/05 06:00
CRDT- 2018/11/03 06:00
PHST- 2018/07/31 00:00 [received]
PHST- 2018/08/27 00:00 [revised]
PHST- 2018/11/06 06:00 [pubmed]
PHST- 2019/06/05 06:00 [medline]
PHST- 2018/11/03 06:00 [entrez]
AID - 10.1002/adma.201804966 [doi]
PST - ppublish
SO - Adv Mater. 2018 Dec;30(52):e1804966. doi: 10.1002/adma.201804966. Epub 2018
Nov 2.
PMID- 30658956
OWN - NLM
STAT- MEDLINE
DCOM- 20190730
LR - 20190730
IS - 1067-2516 (Linking)
VI - 58
IP - 2
DP - 2019 Mar
TI - Open Talar Neck Fracture With Medial Subtalar Joint Dislocation: A Case
Report.
PG - 392-397
LID - S1067-2516(18)30390-9 [pii]
LID - 10.1053/j.jfas.2018.08.049 [doi]
AB - We present a unique case of an open talar neck fracture with medial subtalar
joint
dislocation. This rare and traumatic injury was treated with immediate open
reduction of the subtalar joint and open reduction internal fixation of the
talar
neck fracture. After a follow-up of 2.2 years, highlighted by numerous
complications
including posttraumatic arthritis, soft tissue abscess, and fibrotic
adhesions, the
patient recovered sufficiently to return full activity.
CI - Copyright © 2018 the American College of Foot and Ankle Surgeons. Published
by
Elsevier Inc. All rights reserved.
FAU - Flippin, Mitchell
AU - Flippin M
AD - Podiatric Surgical Resident, Department of Podiatric Surgery, Beaumont
Hospital
Wayne, Wayne, MI. Electronic address: mpflippin@gmail.com.
FAU - Fallat, Lawrence M
AU - Fallat LM
AD - Podiatric Surgical Resident, Department of Podiatric Surgery, Beaumont
Hospital
Wayne, Wayne, MI.
LA - eng
PT - Case Reports
PT - Review
DEP - 20190115
PL - United States
TA - J Foot Ankle Surg
JT - The Journal of foot and ankle surgery : official publication of the American
College
of Foot and Ankle Surgeons
JID - 9308427
MH - *Accidental Falls
MH - Bone Screws
MH - Emergency Service, Hospital
MH - Follow-Up Studies
MH - Fracture Fixation, Internal/instrumentation/*methods
MH - Fractures, Bone/diagnostic imaging/*surgery
MH - Fractures, Open/diagnostic imaging/*surgery
MH - Humans
MH - Injury Severity Score
MH - Joint Dislocations/diagnostic imaging/*surgery
MH - Male
MH - Middle Aged
MH - Soft Tissue Injuries/diagnostic imaging/surgery
MH - Subtalar Joint/injuries/surgery
MH - Talus/diagnostic imaging/injuries/*surgery
OTO - NOTNLM
OT - Hawkins type II
OT - avascular necrosis
OT - open fracture
OT - osteonecrosis
OT - subtalar joint dislocation
OT - talar neck
OT - trauma
EDAT- 2019/01/20 06:00
MHDA- 2019/07/31 06:00
CRDT- 2019/01/20 06:00
PHST- 2017/10/25 00:00 [received]
PHST- 2019/01/20 06:00 [pubmed]
PHST- 2019/07/31 06:00 [medline]
PHST- 2019/01/20 06:00 [entrez]
AID - S1067-2516(18)30390-9 [pii]
AID - 10.1053/j.jfas.2018.08.049 [doi]
PST - ppublish
SO - J Foot Ankle Surg. 2019 Mar;58(2):392-397. doi: 10.1053/j.jfas.2018.08.049.
Epub
2019 Jan 15.
PMID- 31642955
OWN - NLM
STAT- MEDLINE
DCOM- 20200908
LR - 20200908
IS - 0936-8051 (Linking)
VI - 140
IP - 4
DP - 2020 Apr
TI - External iliac artery thrombosis following open reduction of acetabular
fracture: a
case report and literature review.
PG - 481-485
LID - 10.1007/s00402-019-03288-3 [doi]
AB - BACKGROUND: Postoperative thrombosis of the external iliac artery (EIA)
following
open reduction and internal fixation for acetabular fracture is extremely
rare.
PURPOSE: To report a patient with EIA thrombosis following open reduction and
internal fixation using the modified ilioinguinal approach for acetabular

fractures.
STUDY DESIGN: This is a case report of a 69-year-old male with a left
acetabular
fracture who was treated surgically. METHODS: A 69-year-old male presented
with left
hip pain after a 1.5-m fall. Radiographs revealed left acetabular anterior
wall and
posterior hemitransverse fractures with dome impaction. Computed tomography
(CT)
showed atherosclerotic changes in many arteries. Open reduction and internal
fixation were performed using the modified ilioinguinal approach. Adhesion
around
the external iliac vessels was severe, and the external iliac vein (EIV)
ruptured
during exposure. After EIV repair, anatomical reduction was achieved and the
fracture was fixed using a reconstruction plate. Nine hours after surgery,
the left
lower limb showed acute ischemic symptoms. Contrast-enhanced CT indicated
complete
occlusion of the left EIA. The patient was immediately taken for a
thrombectomy via
EIA cut-down using a Fogarty catheter. Postoperatively, he had palpable
dorsalis
pedis and posterior tibial pulses; however, post-reperfusion compartment
syndrome
developed. Fasciotomy of the left leg was performed. RESULTS: At the 2-year
and
4-month follow-up, he was pain-free in his hip and leg. Although he was
walking with
a cane, activity was limited due to a mild foot drop. CONCLUSIONS: It is very
important for surgeons to consider EIA thrombosis as a potential complication
following open reduction and internal fixation. In this case, EIA thrombosis
could
be explained by preoperative atherosclerotic changes and intraoperative
vascular
handling procedures. Preoperative screening and management, and meticulous
surgical
procedures are necessary for patients with a high risk of thrombosis.
FAU - Yamamoto, Norio
AU - Yamamoto N
AD - Department of Orthopedic Surgery, Kagawa Prefectural Central Hospital,
Kagawa,
Japan.
FAU - Noda, Tomoyuki
AU - Noda T
AD - Department of Musculoskeletal Traumatology, Okayama University Graduate
School of
Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku,
Okayama City, 700-8558, Okayama, Japan. tnoda@md.okayama-u.ac.jp.

FAU - Saito, Taichi
AU - Saito T
AD - Department of Orthopaedic Surgery, Okayama University Graduate School of
Medicine,
Dentistry and Pharmaceutical Science, Okayama, Japan.
FAU - Uehara, Takenori
AU - Uehara T
AD - Department of Emergency Healthcare and Disaster Medicine, Okayama University
Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama,
Japan.
FAU - Shimamura, Yasunori
AU - Shimamura Y
AD - Department of Sports Medicine, Okayama University Graduate School of
Medicine,
Dentistry and Pharmaceutical Sciences, Okayama, Japan.
FAU - Ozaki, Toshifumi
AU - Ozaki T
AD - Department of Orthopaedic Surgery, Okayama University Graduate School of
Medicine,
Dentistry and Pharmaceutical Science, Okayama, Japan.
LA - eng
PT - Case Reports
PT - Review
DEP - 20191023
PL - Germany
TA - Arch Orthop Trauma Surg
JT - Archives of orthopaedic and trauma surgery
JID - 9011043
SB - IM
MH - Acetabulum/injuries/surgery
MH - Aged
MH - Hip Fractures/surgery
MH - Humans
MH - *Iliac Artery/physiopathology/surgery
MH - Male
MH - Open Fracture Reduction/*adverse effects
MH - *Thrombosis/etiology/surgery
OTO - NOTNLM
OT - Acetabular fracture
OT - External iliac artery
OT - External iliac vein
OT - Ilioinguinal approach
OT - Occlusion
OT - Thrombosis
EDAT- 2019/10/24 06:00
MHDA- 2020/09/09 06:00
CRDT- 2019/10/24 06:00
PHST- 2019/08/18 00:00 [received]
PHST- 2019/10/24 06:00 [pubmed]
PHST- 2020/09/09 06:00 [medline]
PHST- 2019/10/24 06:00 [entrez]
AID - 10.1007/s00402-019-03288-3 [pii]
AID - 10.1007/s00402-019-03288-3 [doi]
PST - ppublish
SO - Arch Orthop Trauma Surg. 2020 Apr;140(4):481-485. doi: 10.1007/s00402-019-
03288-3.
Epub 2019 Oct 23.
PMID- 21986659
OWN - NLM
STAT- MEDLINE
DCOM- 20120224
LR - 20190608
IS - 1678-7757 (Print)
IS - 1678-7757 (Linking)
VI - 19
IP - 5
DP - 2011 Oct
TI - Histomorphometric analysis of the repair process of autogenous bone grafts
fixed at
rat calvaria with cyanoacrylate.
PG - 529-34
LID - S1678-77572011000500016 [pii]
AB - OBJECTIVE: The purpose of this study was to perform histological and
histometric
analyses of the repair process of autogenous bone grafts fixed at rat
calvaria with
ethyl-cyanoacrylate adhesive. MATERIAL AND METHODS: Thirty-two rats were
divided
into two groups (n=16), Group I - Control and Group II - Adhesive.
Osteotomies were
made at the right parietal bone for graft obtainment using a 4-mm-diameter
trephine
drill. Then, the bone segments were fixed with the adhesive in the parietal
region
of the opposite side to the donor site. After 10 and 30 days, 8 animals of
each
group were euthanized and the calvarias were laboratorially processed for
obtaining
hematoxylin and eosin-stained slides for histological and histometric
analyses.
RESULTS: An intense inflammatory reaction was observed at the 10-day period.
At 30
days, this reaction was less intense, despite the presence of adhesive at the
recipient-site/graft interface. Graft incorporation to the recipient site was
observed only at the control group, which maintained the highest graft size
at 10
and 30 days. CONCLUSIONS: Although the fragment was stable, the presence of
adhesive
in Group II did not allow graft incorporation to the recipient site,
determining a
localized, discrete and persistent inflammatory reaction.
FAU - Esteves, Jônatas Caldeira
AU - Esteves JC
AD - Araraquara Dental School, Univ. Estadual Paulista, Rua Humaitá 1680,
Araraquara, SP,
Brazil. jonatasce@hotmail.com
FAU - Borrasca, Albanir Gabriel
AU - Borrasca AG
FAU - Aranega, Alessandra Marcondes
AU - Aranega AM
FAU - Garcia Junior, Idelmo Rangel
AU - Garcia Junior IR
FAU - Magro Filho, Osvaldo
AU - Magro Filho O
LA - eng
TA - J Appl Oral Sci
JT - Journal of applied oral science : revista FOB
JID - 101189774
RN - 2G95FOH7SF (ethyl 2-cyanoacrylate)
SB - D
SB - IM
MH - Animals
MH - Bone Transplantation/*methods
MH - *Cyanoacrylates
MH - Fracture Healing/drug effects/physiology
MH - Male
MH - Rats
MH - Rats, Wistar
MH - Skull/transplantation
MH - Time Factors
MH - *Tissue Adhesives
PMC - PMC3984202
EDAT- 2011/10/12 06:00
MHDA- 2012/03/01 06:00
CRDT- 2011/10/12 06:00
PHST- 2009/10/26 00:00 [received]
PHST- 2010/10/26 00:00 [accepted]
PHST- 2011/10/12 06:00 [entrez]
PHST- 2011/10/12 06:00 [pubmed]
PHST- 2012/03/01 06:00 [medline]
AID - S1678-77572011000500016 [pii]
AID - 10.1590/s1678-77572011000500016 [doi]
PST - ppublish
SO - J Appl Oral Sci. 2011 Oct;19(5):529-34. doi: 10.1590/s1678-77572011000500016.
PMID- 28919510
OWN - NLM
STAT- MEDLINE
DCOM- 20180611
LR - 20181202
IS - 1742-7061 (Linking)
VI - 63
DP - 2017 Nov
TI - Magnesium (Mg) based interference screws developed for promoting tendon graft
incorporation in bone tunnel in rabbits.

PG -
393-410
LID -
S1742-7061(17)30582-2 [pii]
LID -
10.1016/j.actbio.2017.09.018 [doi]
AB -
How to enhance tendon graft incorporation into bone tunnels for achieving
satisfactory healing outcomes in patients with anterior cruciate ligament
reconstruction (ACLR) is one of the most challenging clinical problems in
orthopaedic sports medicine. Several studies have recently reported the
beneficial
effects of Mg implants in bone fracture healing, indicating the use potential
of Mg
devices in promoting the tendon graft osteointegration. Here, we developed an
innovative Mg-based interference screws for fixation of the tendon graft in

rabbits
underwent ACLR and investigated the biological role of Mg-based implants in
the
graft healing. The titanium (Ti) interference screw was used as the control.
We
demonstrated that Mg interference screw significantly accelerated the
incorporation
of the tendon graft into bone tunnels via multiscale analytical methods
including
scanning electronic microscopy/energy dispersive spectrometer (SEM/EDS),
micro-hardness, micro-Fourier transform infrared spectroscopy (μFTIR), and
histology. Our in vivo study showed that Mg implants enhanced the recruitment
of
bone marrow stromal stem cells (BMSCs) towards peri-implant bone tissue,
which may
be ascribed to the upregulation of local TGF-β1 and PDGF-BB. Besides, the in
vitro
study revealed that higher Mg ions was beneficial to the improvement of
capability
in cell adhesion and osteogenic differentiation of BMSCs. Thus, the
enhancement in
cell migration, cell adhesion and osteogenic differentiation of BMSCs may
contribute
to an improved tendon graft osteointegration in the Mg group. Our findings in
this
work may further facilitate clinical applications of Mg-based interference
screws
for enhancing tendon graft-bone junction healing in patients indicated for
ACLR.
STATEMENT OF SIGNIFICANCE: How to promote tendon-bone junction healing is one
of the
major challenging issues for satisfactory clinical outcomes in patients after
ACL
reconstruction. The improvement of bony ingrowth into the tendon graft-bone
interface can enhance the tendon graft osteointegration. In this study, we
applied
Mg based interference screws to fix the tendon graft in rabbits and found the
use of
Mg screws could accelerate and significantly increase mineralized matrix
formation
at the tendon-bone interface in animals when compared to those with Ti
screws. We
elucidated the mechanism behind the favorable effects of Mg screws on the
graft
healing in both in vitro and in vivo studies from multiscale technologies.
The
optimized interface structure and function in Mg group may be ascribed to the
improved cell migration capability, enhanced cell adhesion strength and

promoted
osteogenic differentiation ability of BMSCs under the stimuli of Mg ions
degraded
from implanted Mg screws. Our findings may help us broaden our thinking in
the
application potential of Mg interference screws in future clinical trials.
reserved.
FAU - Wang, Jiali
AU - Wang J
AD - Musculoskeletal Research Laboratory of Department of Orthopaedics &
Traumatology and
Innovative Orthopaedic Biomaterial and Drug Translational Research Laboratory
of Li
Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong,
Hong
Kong Special Administrative Region.
FAU - Xu, Jiankun
AU - Xu J
Traumatology and
of Li
Hong
FAU - Song, Bin
AU - Song B
AD - Department of Sports Medicine, Sun Yat Sen Memorial Hospital, Sun Yat Sen
University, Guangzhou 510120, PR China.
FAU - Chow, Dick Hokiu
AU - Chow DH
Traumatology and
of Li
Hong
FAU - Shu-Hang Yung, Patrick
AU - Shu-Hang Yung P
Traumatology and
of Li
Hong
FAU - Qin, Ling
AU - Qin L
Traumatology and
of Li
Hong
Kong Special Administrative Region; Center for Translational Medicine
Research and
Development, Institute of Biomedical and Health Engineering, Chinese Academy
of
Sciences, Shenzhen 518055, PR China. Electronic address: qin@ort.cuhk.edu.hk.
LA - eng
DEP - 20170914
PL - England
TA - Acta Biomater
JID - 101233144
RN - 0 (Minerals)
RN - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
RN - I38ZP9992A (Magnesium)
SB - IM
MH - Animals
MH - Anterior Cruciate Ligament/drug effects/surgery
MH - Anterior Cruciate Ligament Reconstruction
MH - *Bone Screws
MH - Bone and Bones/drug effects/*physiology
MH - Corrosion
MH - Hardness
MH - Implants, Experimental
MH - Knee Joint/diagnostic imaging/pathology
MH - Magnesium/*pharmacology
MH - Mesenchymal Stem Cells/cytology/drug effects
MH - Minerals/metabolism
MH - Osteoclasts/drug effects/metabolism
MH - Rabbits
MH - Rats
MH - Tartrate-Resistant Acid Phosphatase/metabolism
MH - Tendons/drug effects/*transplantation
MH - Tomography, X-Ray Computed
OTO - NOTNLM
OT - *ACL
OT - *Biodegradable
OT - *Magnesium
OT - *Structure-function
OT - *Tendon graft-bone junction
EDAT- 2017/09/19 06:00
MHDA- 2018/06/12 06:00
CRDT- 2017/09/19 06:00
PHST- 2017/03/28 00:00 [received]
PHST- 2017/08/27 00:00 [revised]
PHST- 2017/09/13 00:00 [accepted]
PHST- 2017/09/19 06:00 [pubmed]
PHST- 2018/06/12 06:00 [medline]
PHST- 2017/09/19 06:00 [entrez]
AID - S1742-7061(17)30582-2 [pii]
AID - 10.1016/j.actbio.2017.09.018 [doi]
PST - ppublish
SO - Acta Biomater. 2017 Nov;63:393-410. doi: 10.1016/j.actbio.2017.09.018. Epub
2017 Sep
14.
PMID- 30791404
OWN - NLM
STAT- MEDLINE
DCOM- 20190313
LR - 20200225
IS - 1424-8220 (Linking)
VI - 19
IP - 4
DP - 2019 Feb 19
TI - Towards a Non-Invasive Technique for Healing Assessment of Internally Fixated
Femur.
LID - 10.3390/s19040857 [doi]
LID - 857
AB - The lack of a quantitative method to adequately assess fractured bone healing
that
has undergone fixation limits prognostic capabilities on patients' optimal
return to
work. This paper addresses the use of vibrational analysis to monitor the
state of
healing of a plate-screw fixated femur and supplement the current clinical
radiographic assessment. This experimental study involves an osteotomised
composite
femur specimen enclosed by modelling clay to simulate the damping effect of
overlying soft tissues. Epoxy adhesives are applied to the fractured region
and to
simulate the healing process. With the instrumentation described, the cross-
spectrum
and coherence are obtained and analysed in the frequency domain over a period
of
time. The results suggest that it is crucial to analyse the cross-spectrum
and
proposed healing index to quantitatively assess the stages of healing. The
results
also show that the mass loading effect due to modelling clay did not
influence the
proposed healing assessment technique. The findings indicate a potential
non-intrusive technique to evaluate the healing of fractured femur by
utilising the
vibrational responses.
FAU - Chiu, Wing Kong
AU - Chiu WK
AD - Department of Mechanical & Aerospace Engineering, Monash University,
Wellington Rd,
Clayton, VIC 3800, Australia. wing.kong.chiu@monash.edu.
FAU - Vien, Benjamin Steven
AU - Vien BS
AUID- ORCID: 0000-0002-0991-4293
AD - Department of Mechanical & Aerospace Engineering, Monash University,
Wellington Rd,
Clayton, VIC 3800, Australia. ben.vien@monash.edu.
FAU - Russ, Matthias
AU - Russ M
AD - The Alfred Hospital, 55 Commercial Road, Melbourne, VIC 3004, Australia.
M.Russ@alfred.org.au.
AD - National Trauma Research Institute, 89 Commercial Road, Melbourne, VIC 3004,
Australia. M.Russ@alfred.org.au.
FAU - Fitzgerald, Mark
AU - Fitzgerald M
AD - The Alfred Hospital, 55 Commercial Road, Melbourne, VIC 3004, Australia.
M.Fitzgerald@alfred.org.au.
AD - National Trauma Research Institute, 89 Commercial Road, Melbourne, VIC 3004,
Australia. M.Fitzgerald@alfred.org.au.
LA - eng
GR - N00014-16-1-2882/Office of Naval Research/
DEP - 20190219
TA - Sensors (Basel)
JT - Sensors (Basel, Switzerland)
JID - 101204366
RN - 0 (Epoxy Compounds)
SB - IM
MH - Bone Plates
MH - Bone Screws
MH - Cadaver
MH - Epoxy Compounds/*administration & dosage
MH - Femoral Fractures/*drug therapy/physiopathology/surgery
MH - Femur/*drug effects/physiopathology
MH - Finite Element Analysis
MH - Humans
MH - Internal Fixators
MH - *Wound Healing
PMC - PMC6413011
OTO - NOTNLM
OT - dynamic response
OT - fractured femur
OT - healing assessment
OT - internal fixation
OT - spectral analysis
EDAT- 2019/02/23 06:00
MHDA- 2019/03/14 06:00
CRDT- 2019/02/23 06:00
PHST- 2019/01/22 00:00 [received]
PHST- 2019/02/13 00:00 [revised]
PHST- 2019/02/15 00:00 [accepted]
PHST- 2019/02/23 06:00 [entrez]
PHST- 2019/02/23 06:00 [pubmed]
PHST- 2019/03/14 06:00 [medline]
AID - s19040857 [pii]
AID - sensors-19-00857 [pii]
AID - 10.3390/s19040857 [doi]
PST - epublish
SO - Sensors (Basel). 2019 Feb 19;19(4):857. doi: 10.3390/s19040857.
PMID- 29369492
OWN - NLM
STAT- MEDLINE
DCOM- 20190305
LR - 20190305
IS - 1616-5187 (Linking)
VI - 18
IP - 3
DP - 2018 Mar
TI - Biological Activity of an Injectable Biphasic Calcium Phosphate/PMMA Bone
Cement for
Induced Osteogensis in Rabbit Model.
LID - 10.1002/mabi.201700331 [doi]
AB - Polymethylmethacrylate (PMMA) bone cement is widely used in repair of
vertebral
fracture because of its good biomechanical properties and fast curing.
However, the
bioinertness of PMMA cement may cause interfacial loosening, fatigue,
fracture, and
ultimate failure. In this study, biphasic calcium phosphate (BCP) is
introduced into
PMMA cement to prepare an injectable composite bone cement (BCP(x) /PMMA) and
the
content of BCP is optimized to achieve appropriate rate of absorption that
matches
the bone regeneration. The compressive strength of BCP(x) /PMMA bone cement
is found
to comply with the International Standardization Organization standard 5833,
and can
promote biomineralization as well as adhesion, proliferation, and osteogenic
differentiation of Sprague-Dawley rat bone marrow mesenchymal stem cells in
vitro.
Furthermore, in vivo test performed on a rabbit radius defect model
demonstrates
that the presence of BCP can significantly improve the osteogenic efficacy of
PMMA
cement. Therefore, it is anticipated that BCP(x) /PMMA bone cement, as a
promising
injectable biomaterial, is of great potential in bone tissue regeneration.
FAU - Zhang, Xiashiyao
AU - Zhang X
AD - Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical
Instruments,
Department of Biomedical Engineering, School of Engineering, Sun Yat-sen
University,
Guangzhou, 510006, P. R. China.
FAU - Kang, Ting
AU - Kang T
Instruments,
University,
FAU - Liang, Peiqing
AU - Liang P
Instruments,
University,
FAU - Tang, Yong
AU - Tang Y
AD - Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen
University,
FAU - Quan, Changyun
AU - Quan C
Instruments,
University,
LA - eng
DEP - 20180125
PL - Germany
TA - Macromol Biosci
JT - Macromolecular bioscience
JID - 101135941
RN - 0 (Bone Cements)
RN - 0 (Hydroxyapatites)
RN - 0 (hydroxyapatite-beta tricalcium phosphate)
RN - 9011-14-7 (Polymethyl Methacrylate)
SB - IM
MH - Animals
MH - Bone Cements/*chemistry/pharmacology
MH - Bone and Bones/*drug effects/physiology
MH - Compressive Strength
MH - Hydroxyapatites/*pharmacology
MH - Male
MH - Models, Animal
MH - *Osteogenesis
MH - *Polymethyl Methacrylate
MH - Rabbits
MH - Rats
OTO - NOTNLM
OT - *BCPx/PMMA bone cement
OT - *biological activity
OT - *biphasic calcium phosphate
OT - *osteogensis
EDAT- 2018/01/26 06:00
MHDA- 2019/03/06 06:00
CRDT- 2018/01/26 06:00
PHST- 2017/09/29 00:00 [received]
PHST- 2017/12/14 00:00 [revised]
PHST- 2018/01/26 06:00 [pubmed]
PHST- 2019/03/06 06:00 [medline]
PHST- 2018/01/26 06:00 [entrez]
AID - 10.1002/mabi.201700331 [doi]
PST - ppublish
SO - Macromol Biosci. 2018 Mar;18(3). doi: 10.1002/mabi.201700331. Epub 2018 Jan
25.
PMID- 33125971
OWN - NLM
STAT- MEDLINE
DCOM- 20210225
LR - 20210225
IS - 0753-3322 (Linking)
VI - 132
DP - 2020 Dec
TI - Puerarin improves the bone micro-environment to inhibit OVX-induced
osteoporosis via
modulating SCFAs released by the gut microbiota and repairing intestinal
mucosal
integrity.
PG - 110923
LID - S0753-3322(20)31115-X [pii]
LID - 10.1016/j.biopha.2020.110923 [doi]
AB - SCOPE: Half of women over the age of 50 will experience a fracture related
osteoporosis in their lifetime. The common treatment is estrogen replacement
therapy, which can cause many side effects. Puerarin as a phytoestrogen has
been
proven to improve postmenopausal osteoporosis. However, the mechanisms of
anti-osteoporosis remain unclear due to its low bioavailability. The aim of
this
study is to investigate whether the anti-osteoporosis effects of puerarin are
related to modulations in the gut microbiota and focus on the mechanism of

gut /
bone axis. METHODS: We established ovariectomized (OVX) rats as osteoporosis
model.
The femur was analyzed by microcomputed tomography (μ-CT) and we measured
serum
biochemical indices and inflammatory factors. 16S rRNA sequencing was
employed to
evaluate the gut microbiota composition in the fecal samples. Short-chain
fatty
acids (SCFAs) was analyzed by GC. The expression of intestinal inflammatory
factors
and adhesion proteins was confirmed by western blotting and qPCR. RESULTS:
Puerarin
increased the BMD and improved the intestinal mucosal integrity to reduce the
systemic inflammation. The disorder of gut microbiota was improved and its
metabolites SCFAs were elevated. Metabolic pathways such as amino acid
metabolism,
LPS biosynthesis and butyrate metabolism were enriched. CONCLUSION: Puerarin
treatment modulated the gut microbiota disorder to elicit the anti-
osteoporosis
effects in OVX rats, by improving the bone micro-environment via regulating
the
SCFAs levels and repairing the intestinal mucosal integrity.
CI - Copyright © 2020 The Author(s). Published by Elsevier Masson SAS.. All rights
reserved.
FAU - Li, Bo
AU - Li B
AD - School of Pharmacy, China Medical University, Shenyang, 110122, China;
Liaoning Key
Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation,
Shenyang, 110122, China.
FAU - Liu, Mingyan
AU - Liu M
Liaoning Key
FAU - Wang, Yu
AU - Wang Y
Liaoning Key
FAU - Gong, Shiqiang
AU - Gong S
Liaoning Key
FAU - Yao, Weifan
AU - Yao W
Liaoning Key
FAU - Li, Wenshuai
AU - Li W
Liaoning Key
FAU - Gao, Hua
AU - Gao H
Division of
Pharmacology Laboratory, National Institutes for Food and Drug Control,
Beijing,
102629, China. Electronic address: huag55@163.com.
FAU - Wei, Minjie
AU - Wei M
Liaoning Key
Shenyang, 110122, China. Electronic address: minjie_wei@163.com.
LA - eng
DEP - 20201028
PL - France
TA - Biomed Pharmacother
JT - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN - 0 (Fatty Acids, Volatile)
RN - 0 (Inflammation Mediators)
RN - 0 (Isoflavones)
RN - Z9W8997416 (puerarin)
SB - IM
MH - Animals
MH - Bacteria/*drug effects/metabolism
MH - Bone Density/drug effects
MH - Bone Remodeling/*drug effects
MH - Colon/*drug effects/microbiology/pathology
MH - Dysbiosis
MH - Fatty Acids, Volatile/*metabolism
MH - Female
MH - Femur/*drug effects/metabolism/pathology
MH - Gastrointestinal Microbiome/*drug effects
MH - Humans
MH - Inflammation Mediators/metabolism
MH - Intestinal Mucosa/*drug effects/microbiology/pathology
MH - Isoflavones/*pharmacology
MH - Osteoporosis, Postmenopausal/metabolism/microbiology/pathology/*prevention &
control
MH - Ovariectomy
OTO - NOTNLM
OT - Gut microbiota
OT - Intestinal mucosal integrity
OT - Osteoporosis
OT - Puerarin
OT - SCFAs
EDAT- 2020/10/31 06:00
MHDA- 2021/02/26 06:00
CRDT- 2020/10/30 20:09
PHST- 2020/08/11 00:00 [received]
PHST- 2020/10/15 00:00 [revised]
PHST- 2020/10/20 00:00 [accepted]
PHST- 2020/10/31 06:00 [pubmed]
PHST- 2021/02/26 06:00 [medline]
PHST- 2020/10/30 20:09 [entrez]
AID - S0753-3322(20)31115-X [pii]
AID - 10.1016/j.biopha.2020.110923 [doi]
PST - ppublish
SO - Biomed Pharmacother. 2020 Dec;132:110923. doi: 10.1016/j.biopha.2020.110923.
Epub
2020 Oct 28.
PMID- 26814464
OWN - NLM
STAT- MEDLINE
DCOM- 20160701
LR - 20181113
IS - 1678-7757 (Print)
IS - 1678-7757 (Linking)
VI - 23
IP - 6
DP - 2015 Nov-Dec
TI - Effect of collagen sponge and fibrin glue on bone repair.
PG - 623-8
LID - S1678-77572015000600623 [pii]
LID - 10.1590/1678-775720150374 [doi]
AB - The ability of hemostatic agents to promote bone repair has been investigated
using
in vitro and in vivo models but, up to now, the results are inconclusive.
Objective
In this context, the aim of this study was to compare the potential of bone
repair
of collagen sponge with fibrin glue in a rat calvarial defect model. MATERIAL
AND
METHODS: Defects of 5 mm in diameter were created in rat calvariae and
treated with
either collagen sponge or fibrin glue; untreated defects were used as
control. At 4
and 8 weeks, histological analysis and micro-CT-based histomorphometry were
carried
out and data were compared by two-way ANOVA followed by Student-Newman-Keuls
test
when appropriated (p≤0.05). RESULTS: Three-dimensional reconstructions showed
increased bone formation in defects treated with either collagen sponge or

fibrin
glue compared with untreated defects, which was confirmed by the histological
analysis. Morphometric parameters indicated the progression of bone formation

from 4
to 8 weeks. Additionally, fibrin glue displayed slightly higher bone
formation rate
when compared with collagen sponge. CONCLUSION: Our results have shown the
benefits
of using collagen sponge and fibrin glue to promote new bone formation in rat
calvarial bone defects, the latter being discreetly more advantageous.

FAU - Santos, Thiago de Santana
AU - Santos Tde S
AD - Laboratório de Cultura de Células, Faculdade de Odontologia de Ribeirão
Preto,
Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
FAU - Abuna, Rodrigo Paolo Flores
AU - Abuna RP
Preto,
FAU - Almeida, Adriana Luisa Gonçalves de
AU - Almeida AL
Preto,
FAU - Beloti, Marcio Mateus
AU - Beloti MM
Preto,
FAU - Rosa, Adalberto Luiz
AU - Rosa AL
Preto,
LA - eng
TA - J Appl Oral Sci
JT - Journal of applied oral science : revista FOB
JID - 101189774
RN - 0 (Hemostatics)
SB - D
SB - IM
MH - Animals
MH - Bone Regeneration/*drug effects
MH - Collagen/*pharmacology
MH - Hemostatics/*pharmacology
MH - Male
MH - Osteogenesis/*drug effects
MH - Rats, Wistar
MH - Reproducibility of Results
MH - Skull/drug effects/injuries
MH - Swine
MH - Time Factors
PMC - PMC4716700
COIS- Disclosure statement All authors confirm that there is no conflict of
interest
associated with this study and there has been no financial support that could
have
influenced the results.
EDAT- 2016/01/28 06:00
MHDA- 2016/07/02 06:00
CRDT- 2016/01/28 06:00
PHST- 2015/08/10 00:00 [received]
PHST- 2015/09/25 00:00 [accepted]
PHST- 2016/01/28 06:00 [entrez]
PHST- 2016/01/28 06:00 [pubmed]
PHST- 2016/07/02 06:00 [medline]
AID - S1678-77572015000600623 [pii]
AID - 1678-775720150374 [pii]
AID - 10.1590/1678-775720150374 [doi]
PST - ppublish
SO - J Appl Oral Sci. 2015 Nov-Dec;23(6):623-8. doi: 10.1590/1678-775720150374.
PMID- 30853660
OWN - NLM
STAT- MEDLINE
DCOM- 20200701
LR - 20200701
IS - 1873-2763 (Linking)
VI - 122
DP - 2019 May
TI - GIT1 is critical for formation of the CD31(hi)Emcn(hi) vessel subtype in
coupling
osteogenesis with angiogenesis via modulating preosteoclasts secretion of
PDGF-BB.
PG - 218-230
LID - S8756-3282(19)30082-1 [pii]
LID - 10.1016/j.bone.2019.03.006 [doi]
AB - G protein-coupled receptor kinase 2 interacting protein-1 (GIT1) is a
scaffold
protein that plays a vital role in bone modeling and remodeling during
osteogenesis
coupled with angiogenesis. Recent studies have shown that a specialized
subset of
vascular endothelium strongly positive for CD31 and Endomucin
(CD31(hi)Emcn(hi)) is
coupled with anabolic bone formation. Based on our previous finding that GIT1
knockout (GIT1 KO) mice have impaired angiogenesis and bone formation, we
hypothesized that GIT1 affects formation of the CD31(hi)Emcn(hi) vessel
subtype. In
the current study, GIT1 knockout (GIT1 KO) mice displayed a significant
decrease in
trabecular bone mass and CD31(hi)Emcn(hi) vessel number, compared to their
wild-type
counterparts. In the fracture healing mouse model, GIT1 KO mice contained a
lower
number of CD31(hi)Emcn(hi) vessels in fracture callus at days 7 and 14.
However, no
significant differences in the number of preosteoclasts in bone marrow,
trabecular
bone and callus in GIT1 KO mice were observed, compared with wild-type mice.
Notably, concentrations of serum platelet-derived growth factor-BB(PDGF-BB)
secreted
by preosteoclasts associated with CD31(hi)Emcn(hi) vessel formation were
lower in
GIT1 KO mice. In addition, PDGF-BB-associated expression of phosphorylated
extracellular signal-regulated kinase- 1/2 (ERK1/2) and specificity protein 1
(SP1)
was significantly decreased in preosteoclasts of GIT1 KO mice. These results
collectively suggest that GIT1 is a critical participant in formation of the
CD31(hi)Emcn(hi) vessel subtype, highlighting a novel biologic function of
this
scaffold protein in preosteoclasts.
CI - Copyright © 2019. Published by Elsevier Inc.
FAU - Xu, Tao
AU - Xu T
AD - Department of Orthopedic, the First Affiliated Hospital of Nanjing Medical
University, 300 Guangzhou Rd., Nanjing 210029, China.
FAU - Luo, YongJun
AU - Luo Y
FAU - Kong, FanQi
AU - Kong F
FAU - Lv, Bin
AU - Lv B
AD - Department of Orthopedics, The Affiliated People's Hospital with Jiangsu
University,
Zhenjiang, Jiangsu Province 212000, China.
FAU - Zhao, ShuJie
AU - Zhao S
FAU - Chen, Jian
AU - Chen J
FAU - Liu, Wei
AU - Liu W
FAU - Cheng, Lin
AU - Cheng L
FAU - Zhou, Zheng
AU - Zhou Z
FAU - Zhou, ZhiMin
AU - Zhou Z
FAU - Huang, YiFan
AU - Huang Y
FAU - Li, LinWei
AU - Li L
FAU - Zhao, Xuan
AU - Zhao X
FAU - Qian, DingFei
AU - Qian D
FAU - Fan, Jin
AU - Fan J
University, 300 Guangzhou Rd., Nanjing 210029, China. Electronic address:
fanjin@njmu.edu.cn.
FAU - Yin, GuoYong
AU - Yin G
University, 300 Guangzhou Rd., Nanjing 210029, China. Electronic address:
guoyong_yin@sina.com.
LA - eng
DEP - 20190307
PL - United States
TA - Bone
JT - Bone
JID - 8504048
RN - 0 (Cell Cycle Proteins)
RN - 0 (Culture Media, Conditioned)
RN - 0 (Emcn protein, mouse)
RN - 0 (GTPase-Activating Proteins)
RN - 0 (Git1 protein, mouse)
RN - 0 (Sialoglycoproteins)
RN - 0 (Sp1 Transcription Factor)
RN - 1B56C968OA (Becaplermin)
SB - IM
MH - Animals
MH - Becaplermin/*metabolism
MH - Cell Cycle Proteins/*metabolism
MH - Cell Movement/drug effects
MH - Culture Media, Conditioned/pharmacology
MH - Endothelium/metabolism
MH - GTPase-Activating Proteins/*metabolism
MH - HEK293 Cells
MH - Humans
MH - Mice, Knockout
MH - Mitogen-Activated Protein Kinase 3/metabolism
MH - *Neovascularization, Physiologic/drug effects
MH - Osteoclasts/drug effects/*metabolism
MH - *Osteogenesis/drug effects
MH - Platelet Endothelial Cell Adhesion Molecule-1/*metabolism
MH - Sialoglycoproteins/*metabolism
MH - Sp1 Transcription Factor/metabolism
OTO - NOTNLM
OT - *Bone formation
OT - *Endomucin
OT - *Endothelial cells
OT - *GIT1
OT - *PDGF-BB
OT - *Preosteoclasts
EDAT- 2019/03/12 06:00
MHDA- 2020/07/02 06:00
CRDT- 2019/03/12 06:00
PHST- 2018/12/25 00:00 [received]
PHST- 2019/03/06 00:00 [revised]
PHST- 2019/03/06 00:00 [accepted]
PHST- 2019/03/12 06:00 [pubmed]
PHST- 2020/07/02 06:00 [medline]
PHST- 2019/03/12 06:00 [entrez]
AID - S8756-3282(19)30082-1 [pii]
AID - 10.1016/j.bone.2019.03.006 [doi]
PST - ppublish
SO - Bone. 2019 May;122:218-230. doi: 10.1016/j.bone.2019.03.006. Epub 2019 Mar 7.
PMID- 30731438
OWN - NLM
STAT- MEDLINE
DCOM- 20191021
LR - 20191022
IS - 1748-6041 (Linking)
VI - 14
IP - 3
DP - 2019 Mar 14
TI - Strontium ranelate simultaneously improves the radiopacity and osteogenesis
of
calcium phosphate cement.
PG - 035005
LID - 10.1088/1748-605X/ab052d [doi]
AB - In a minimally invasive surgery of osteoporotic fractures, high radiopacity
is
necessary to monitor the delivery and positioning of injectable cements and
good
osteogenesis is indispensable. In this work, strontium ranelate (SrR), an
agent for
treating osteoporosis, is firstly used as a radiopaque agent for calcium
phosphate
cement (CPC). The addition of SrR does not affect the hydration products of
CPC, but
prolonged the setting time and decreased the compressive strength. The
injectability
of the cement was higher than 85% when SrR content is more than 10 wt%. The
radiopacity of CPC is significantly improved by SrR and higher than cortical
bone
when the content of SrR is more than 5 wt%. The concentration of Sr ions
released
from CPC is increased by the increasing content of SrR, which is among 17-
1329 μM.
Moreover, CPCs with SrR significantly promote the osteogenic differentiation
of
mouse bone marrow mesenchymal stem cells and inhibit the osteoclastogenic
differentiation of RAW264.7 cells. Based on its good radiopacity and
osteogenesis,
suppressed osteoclastogenesis and appropriate physicochemical properties, the
radiopaque CPC with more than 10 wt% SrR is prospective to be a promising

biomaterial for osteoporotic fracture repairing in minimal invasive surgery.
FAU - Wu, Tingting
AU - Wu T
AD - National Engineering Research Center for Tissue Restoration and
Reconstruction,
South China University of Technology, Guangzhou 510006, People's Republic of
China.
Institute of Orthopedic Diseases and Center for Joint Surgery and Sports
Medicine,
The First Affiliated Hospital of Jinan University, Guangzhou 510630, People's
Republic of China. School of Materials Science and Engineering, South China

University of Technology, Guangzhou 510640, People's Republic of China.
FAU - Yang, Shue
AU - Yang S
FAU - Lu, Teliang
AU - Lu T
FAU - He, Fupo
AU - He F
FAU - Zhang, Jing
AU - Zhang J
FAU - Shi, Haishan
AU - Shi H
FAU - Lin, Zefeng
AU - Lin Z
FAU - Ye, Jiandong
AU - Ye J
LA - eng
DEP - 20190314
PL - England
TA - Biomed Mater
JID - 101285195
RN - 0 (Calcium Phosphates)
RN - 0 (Culture Media)
RN - 0 (Ions)
RN - 0 (Thiophenes)
RN - 04NQ160FRU (strontium ranelate)
RN - 97Z1WI3NDX (calcium phosphate)
SB - IM
MH - Animals
MH - Bone Cements/*chemistry
MH - Bone Marrow Cells/cytology
MH - Calcium Phosphates/*chemistry
MH - Cell Adhesion
MH - Cell Survival
MH - Culture Media
MH - Gene Expression Profiling
MH - Ions
MH - Mice
MH - Minimally Invasive Surgical Procedures
MH - Osteoclasts/cytology
MH - Osteoporosis
MH - Rheology
MH - Thiophenes/*chemistry
EDAT- 2019/02/08 06:00
MHDA- 2019/10/23 06:00
CRDT- 2019/02/08 06:00
PHST- 2019/02/08 06:00 [pubmed]
PHST- 2019/10/23 06:00 [medline]
PHST- 2019/02/08 06:00 [entrez]
AID - 10.1088/1748-605X/ab052d [doi]
PST - epublish
SO - Biomed Mater. 2019 Mar 14;14(3):035005. doi: 10.1088/1748-605X/ab052d.
PMID- 25967044
OWN - NLM
STAT- MEDLINE
DCOM- 20160904
LR - 20181202
IS - 0884-0431 (Print)
IS - 0884-0431 (Linking)
VI - 30
IP - 11
DP - 2015 Nov
TI - BMP2 Regulation of CXCL12 Cellular, Temporal, and Spatial Expression is
Essential
During Fracture Repair.
PG - 2014-27
LID - 10.1002/jbmr.2548 [doi]
AB - The cellular and humoral responses that orchestrate fracture healing are
still
elusive. Here we report that bone morphogenic protein 2 (BMP2)-dependent
fracture
healing occurs through a tight control of chemokine C-X-C motif-ligand-12
(CXCL12)
cellular, spatial, and temporal expression. We found that the fracture repair
process elicited an early site-specific response of CXCL12(+)-BMP2(+)

endosteal
cells and osteocytes that was not present in unfractured bones and gradually
decreased as healing progressed. Absence of a full complement of BMP2 in
mesenchyme
osteoprogenitors (BMP2(cKO/+)) prevented healing and led to a dysregulated
temporal
and cellular upregulation of CXCL12 expression associated with a deranged
angiogenic
response. Healing was rescued when BMP2(cKO/+) mice were systemically treated
with
AMD3100, an antagonist of CXCR4 and agonist for CXCR7 both receptors for
CXCL12. We
further found that mesenchymal stromal cells (MSCs), capable of delivering
BMP2 at
the endosteal site, restored fracture healing when transplanted into
BMP2(cKO/+)
mice by rectifying the CXCL12 expression pattern. Our in vitro studies showed
that
in isolated endosteal cells, BMP2, while inducing osteoblastic
differentiation,
stimulated expression of pericyte markers that was coupled with a decrease in
CXCL12. Furthermore, in isolated BMP2(cKO/cKO) endosteal cells, high

expression
levels of CXCL12 inhibited osteoblastic differentiation that was restored by
AMD3100
treatment or coculture with BMP2-expressing MSCs that led to an upregulation
of
pericyte markers while decreasing platelet endothelial cell adhesion molecule
(PECAM). Taken together, our studies show that following fracture, a
CXCL12(+)-BMP2(+) perivascular cell population is recruited along the
endosteum,
then a timely increase of BMP2 leads to downregulation of CXCL12 that is
essential
to determine the fate of the CXCL12(+)-BMP2(+) to osteogenesis while
departing their
supportive role to angiogenesis. Our findings have far-reaching implications
for
understanding mechanisms regulating the selective recruitment of distinct
cells into
the repairing niches and the development of novel pharmacological (by
targeting
BMP2/CXCL12) and cellular (MSCs, endosteal cells) interventions to promote
fracture
healing.
FAU - Myers, Timothy J
AU - Myers TJ
AD - Division of Endocrinology, Department of Pediatrics, University of North
Carolina at
Chapel Hill, Chapel Hill, NC, USA.
FAU - Longobardi, Lara
AU - Longobardi L
Carolina at
FAU - Willcockson, Helen
AU - Willcockson H
Carolina at
FAU - Temple, Joseph D
AU - Temple JD
Carolina at
AD - Department of Pediatrics, Rush University Medical Center, Chicago, IL, USA.
FAU - Tagliafierro, Lidia
AU - Tagliafierro L
Carolina at
FAU - Ye, Ping
AU - Ye P
Carolina at
FAU - Li, Tieshi
AU - Li T
Carolina at
FAU - Esposito, Alessandra
AU - Esposito A
Carolina at
FAU - Moats-Staats, Billie M
AU - Moats-Staats BM
Carolina at
FAU - Spagnoli, Anna
AU - Spagnoli A
Carolina at
LA - eng
GR - F32 AR060113/AR/NIAMS NIH HHS/United States
GR - R01 DK070929/DK/NIDDK NIH HHS/United States
DEP - 20150615
Society
JID - 8610640
RN - 0 (Chemokine CXCL12)
SB - IM
MH - Animals
MH - Cell Separation
MH - Chemokine CXCL12/*metabolism
MH - Fractures, Bone/metabolism/pathology
MH - Mesenchymal Stem Cell Transplantation
MH - Mesenchymal Stem Cells/metabolism
MH - Mice, Knockout
MH - Time Factors
PMC - PMC4970512
MID - NIHMS805353
OTO - NOTNLM
OT - BMP2
OT - CXCL12
OT - ENDOSTEAL CELLS
OT - FRACTURE REPAIR
OT - MESENCHYMAL STROMAL CELLS
COIS- Disclosures All authors state that they have no conflicts of interest.
EDAT- 2015/05/15 06:00
MHDA- 2016/09/07 06:00
CRDT- 2015/05/14 06:00
PHST- 2014/10/15 00:00 [received]
PHST- 2015/04/27 00:00 [revised]
PHST- 2015/05/05 00:00 [accepted]
PHST- 2015/05/14 06:00 [entrez]
PHST- 2015/05/15 06:00 [pubmed]
PHST- 2016/09/07 06:00 [medline]
AID - 10.1002/jbmr.2548 [doi]
PST - ppublish
SO - J Bone Miner Res. 2015 Nov;30(11):2014-27. doi: 10.1002/jbmr.2548. Epub 2015
Jun 15.
PMID- 32319614
OWN - NLM
STAT- MEDLINE
DCOM- 20210203
LR - 20210203
IS - 1791-2997 (Print)
IS - 1791-2997 (Linking)
VI - 21
IP - 4
DP - 2020 Apr
TI - Identification of key microRNAs and target genes for the diagnosis of bone
nonunion.
PG - 1921-1933
LID - 10.3892/mmr.2020.10996 [doi]
AB - A number of recent studies have highlighted the causes of bone nonunion (BN),
however, the rate of BN incidence continues to rise and available therapeutic
options to treat this condition remain limited. Thus, to prevent disease

progression
and improve patient prognosis, it is vital that BN, or the risk thereof, be
accurately identified in a timely manner. In the present study,
bioinformatics
analyses were used to screen for the differentially expressed genes (DEGs)
and
differentially expressed miRNAs (DEMs) between patients with BN and those
with bone
union, using data from the Gene Expression Omnibus database. Furthermore,
clinical
samples were collected and analyzed by reverse transcription-quantitative PCR
and
western blotting. In vitro and in vivo experiments were carried out to
confirm the
relationship between BN and the DEGs of interest, in addition to being used
to
explore the underlying molecular mechanism of BN. Functional enrichment
analysis of
the downregulated DEGs revealed them to be enriched for genes associated with
'ECM-receptor interactions', 'focal adhesion', 'and the calcium signaling

pathway'.
When comparing DEM target genes with these DEGs, nine DEGs were identified as
putative DEM targets, where hsa-microRNA (miR)-1225-5p-CCNL2,

hsa-miR-339-5p-PRCP,
and hsa-miR-193a-3p-mitogen-activated protein kinase 10 (MAPK10) were the
only three
pairs which were associated with decreased gene expression levels.
Furthermore,
hsa-miR-193a-3p was demonstrated to induce BN by targeting MAPK10.
Collectively, the
results of the present study suggest that hsa-miR-193a-3p may be a viable
biomarker
of BN.
FAU - Xiong, Yuan
AU - Xiong Y
AD - Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong
University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
FAU - Cao, Faqi
AU - Cao F
FAU - Chen, Lang
AU - Chen L
FAU - Yan, Chenchen
AU - Yan C
FAU - Zhou, Wu
AU - Zhou W
FAU - Chen, Yanyan
AU - Chen Y
AD - Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei
430071,
P.R. China.
FAU - Endo, Yori
AU - Endo Y
AD - Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical
School,
Boston, MA 02215, USA.
FAU - Leng, Xingzhu
AU - Leng X
AD - Department of Biomedical Sciences, UMC Utrecht, Utrecht University, Utrecht,
3508
GA, The Netherlands.
FAU - Mi, Bobin
AU - Mi B
FAU - Liu, Guohui
AU - Liu G
LA - eng
DEP - 20200221
TA - Mol Med Rep
JT - Molecular medicine reports
JID - 101475259
RN - 0 (MicroRNAs)
SB - IM
MH - Animals
MH - Down-Regulation/genetics
MH - Fracture Healing/genetics
MH - Fractures, Ununited/*diagnosis/*genetics
MH - *Gene Expression Regulation
MH - Gene Ontology
MH - Gene Regulatory Networks
MH - Humans
MH - Male
MH - MicroRNAs/*genetics/metabolism
MH - Osteoblasts/metabolism
MH - Protein Interaction Maps/genetics
PMC - PMC7057810
EDAT- 2020/04/23 06:00
MHDA- 2021/02/04 06:00
CRDT- 2020/04/23 06:00
PHST- 2019/08/06 00:00 [received]
PHST- 2019/12/12 00:00 [accepted]
PHST- 2020/04/23 06:00 [pubmed]
PHST- 2021/02/04 06:00 [medline]
PHST- 2020/04/23 06:00 [entrez]
AID - mmr-21-04-1921 [pii]
AID - 10.3892/mmr.2020.10996 [doi]
PST - ppublish
SO - Mol Med Rep. 2020 Apr;21(4):1921-1933. doi: 10.3892/mmr.2020.10996. Epub 2020
Feb
21.
PMID- 25580247
OWN - NLM
LR - 20191120
IS - 2050-750X (Print)
VI - 3
DP - 2015 Jan 21
TI - Development of Injectable Citrate-Based Bioadhesive Bone Implants.
PG - 387-398
AB - Injectable bone implants have been widely used in bone tissue repairs
including the
treatment of comminuted bone fractures (CBF). However, most injectable bone
implants
are not suitable for the treatment of CBF due to their weak tissue adhesion
strengths and minimal osteoinduction. Citrate has been recently reported to
promote
bone formation through enhanced bioceramic integration and osteoinductivity.
Herein,
a novel injectable citrate-based mussel-inspired bioadhesive hydroxyapatite
(iCMBA/HA) bone substitute was developed for CBF treatment. iCMBA/HA can be
set
within 2-4 minutes and the as-prepared (wet) iCMBA/HA possess low swelling
ratios,
compressive mechanical strengths of up to 3.2±0.27 MPa, complete degradation
in 30
days, suitable biocompatibility, and osteoinductivity. This is also the first
time
to demonstrate that citrate supplementation in osteogenic medium and citrate
released from iCMBA/HA degradation can promote the mineralization of
osteoblastic
committed human mesenchymal stem cells (hMSCs). In vivo evaluation of
iCMBA/HA in a
rabbit comminuted radial fracture model showed significantly increased bone
formation with markedly enhanced three-point bending strength compared to the
negative control. Neovascularization and bone ingrowth as well as highly

organized
bone formation were also observed showing the potential of iCMBA/HA in
treating CBF.
FAU - Xie, Denghui
AU - Xie D
AD - Department of Orthopedic Surgery, The Third Affiliated Hospital of Southern
Medical
University; Academy of Orthopedics, Guangdong Province; Biology Department,
Southern
Medical University, Guangzhou, 510515, China ; Department of Biomedical
Engineering,
Materials Research Institutes, The Huck Institutes of The Life Sciences, The
Pennsylvania State University, University Park 16802, USA.
FAU - Guo, Jinshan
AU - Guo J
AD - Department of Biomedical Engineering, Materials Research Institutes, The Huck
Institutes of The Life Sciences, The Pennsylvania State University,

University Park
16802, USA.
FAU - Mehdizadeh, Mohammadreza
AU - Mehdizadeh M

University Park
16802, USA.
FAU - Tran, Richard T
AU - Tran RT

University Park
16802, USA.
FAU - Chen, Ruisong
AU - Chen R
Medical
Southern
Medical University, Guangzhou, 510515, China.
FAU - Sun, Dawei
AU - Sun D
Medical
Southern
FAU - Qian, Guoying
AU - Qian G
AD - Department of Biology, College of Biological and Environmental Sciences,
Zhejiang
Wanli University, Ningbo 315100, China.
FAU - Jin, Dadi
AU - Jin D
Medical
Southern
FAU - Bai, Xiaochun
AU - Bai X
Medical
Southern
FAU - Yang, Jian
AU - Yang J
Medical
Southern
Medical University, Guangzhou, 510515, China ; Department of Biomedical
Engineering,
Materials Research Institutes, The Huck Institutes of The Life Sciences, The
Pennsylvania State University, University Park 16802, USA.
LA - eng
GR - R01 CA182670/CA/NCI NIH HHS/United States
GR - R01 EB012575/EB/NIBIB NIH HHS/United States
GR - R01 HL118498/HL/NHLBI NIH HHS/United States
TA - J Mater Chem B
JT - Journal of materials chemistry. B
JID - 101598493
PMC - PMC4286886
MID - NIHMS640365
EDAT- 2015/01/13 06:00
MHDA- 2015/01/13 06:01
CRDT- 2015/01/13 06:00
PHST- 2015/01/13 06:00 [entrez]
PHST- 2015/01/13 06:00 [pubmed]
PHST- 2015/01/13 06:01 [medline]
AID - 10.1039/C4TB01498G [doi]
PST - ppublish
SO - J Mater Chem B. 2015 Jan 21;3:387-398. doi: 10.1039/C4TB01498G.
PMID- 30248646
OWN - NLM
STAT- MEDLINE
DCOM- 20191014
LR - 20191201
IS - 0142-9612 (Print)
IS - 0142-9612 (Linking)
VI - 185
DP - 2018 Dec
TI - 3D printed biofunctionalized scaffolds for microfracture repair of cartilage
defects.
PG - 219-231
LID - S0142-9612(18)30659-8 [pii]
LID - 10.1016/j.biomaterials.2018.09.022 [doi]
AB - While articular cartilage defects affect millions of people worldwide from
adolescents to adults, the repair of articular cartilage defects still
remains
challenging due to the limited endogenous regeneration of the tissue and poor
integration with implants. In this study, we developed a 3D-printed scaffold

functionalized with aggrecan that supports the cellular fraction of bone
marrow
released from microfracture, a widely used clinical procedure, and
demonstrated
tremendous improvement of regenerated cartilage tissue quality and joint
function in
a lapine model. Optical coherence tomography (OCT) revealed doubled thickness
of the
regenerated cartilage tissue in the group treated with our aggrecan
functionalized
scaffold compared to standard microfracture treatment. H&E staining showed
366 ± 95
chondrocytes present in the unit area of cartilage layer with the support of
bioactive scaffold, while conventional microfracture group showed only
112 ± 26
chondrocytes. The expression of type II collagen appeared almost 10 times
higher
with our approach compared to normal microfracture, indicating the potential
to
overcome the fibro-cartilage formation associated with the current
microfracture
approach. The therapeutic effect was also evaluated at joint function level.
The
mobility was evaluated using a modified Basso, Beattie and Bresnahan (BBB)
scale.
While the defect control group showed no movement improvement over the course
of
study, all experimental groups showed a trend of increasing scores over time.
The
present work developed an effective method to regenerate critical articular
defects
by combining a 3D-printed therapeutic scaffold with the microfracture
surgical
procedure. This biofunctionalized acellular scaffold has great potential to
be
applied as a supplement for traditional microfracture to improve the quality
of
cartilage regeneration in a cost and labor effective way.
FAU - Guo, Ting
AU - Guo T
AD - Fischell Department of Bioengineering, University of Maryland, College Park,
MD USA;
Center for Engineering Complex Tissues, University of Maryland, College Park,
MD
USA.
FAU - Noshin, Maeesha
AU - Noshin M
MD USA;
MD
USA.
FAU - Baker, Hannah B
AU - Baker HB
MD USA;
MD
USA.
FAU - Taskoy, Evin
AU - Taskoy E
AD - Department of Orthopaedics, University of Maryland School of Medicine,
Baltimore, MD
USA.
FAU - Meredith, Sean J
AU - Meredith SJ
Baltimore, MD
USA.
FAU - Tang, Qinggong
AU - Tang Q
MD USA.
FAU - Ringel, Julia P
AU - Ringel JP
MD USA;
MD
USA.
FAU - Lerman, Max J
AU - Lerman MJ
AD - Center for Engineering Complex Tissues, University of Maryland, College Park,
MD
USA; Department of Materials Science and Engineering, University of Maryland,
College Park, MD USA; Surface and Trace Chemical Analysis Group, Materials
Measurement Lab, National Institute of Standards and Technology,
Gaithersburg, MD
USA.
FAU - Chen, Yu
AU - Chen Y
MD USA.
FAU - Packer, Jonathan D
AU - Packer JD
Baltimore, MD
USA.
FAU - Fisher, John P
AU - Fisher JP
MD USA;
MD
USA. Electronic address: jpfisher@umd.edu.
LA - eng
GR - P41 EB023833/EB/NIBIB NIH HHS/United States
PT - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20180914
TA - Biomaterials
JT - Biomaterials
JID - 8100316
RN - 0 (Aggrecans)
RN - 0 (Immobilized Proteins)
SB - IM
MH - Aggrecans/*chemistry
MH - Animals
MH - Bioprinting
MH - Cartilage, Articular/cytology/*injuries
MH - Cell Adhesion
MH - Cell Line
MH - Chondrocytes/cytology
MH - Chondrogenesis
MH - Female
MH - Fractures, Stress/*therapy
MH - Humans
MH - Immobilized Proteins/*chemistry
MH - Printing, Three-Dimensional
MH - Rabbits
PMC - PMC6186501
MID - NIHMS1507999
OTO - NOTNLM
OT - *Aggrecan
OT - *Articular cartilage
OT - *Custom fabrication
OT - *Extrusion 3D printing
OT - *Microfracture
OT - *Poly(l-lactide-co-ε-caprolactone)
OT - *Scaffold
EDAT- 2018/09/25 06:00
MHDA- 2019/10/15 06:00
CRDT- 2018/09/25 06:00
PHST- 2018/07/20 00:00 [received]
PHST- 2018/08/27 00:00 [revised]
PHST- 2018/09/13 00:00 [accepted]
PHST- 2018/09/25 06:00 [pubmed]
PHST- 2019/10/15 06:00 [medline]
PHST- 2018/09/25 06:00 [entrez]
AID - S0142-9612(18)30659-8 [pii]
AID - 10.1016/j.biomaterials.2018.09.022 [doi]
PST - ppublish
SO - Biomaterials. 2018 Dec;185:219-231. doi: 10.1016/j.biomaterials.2018.09.022.
Epub
2018 Sep 14.
PMID- 29619243
OWN - NLM
LR - 20200929
IS - 1226-4601 (Print)
IS - 1226-4601 (Linking)
VI - 22
DP - 2018
TI - Two-dimensional material-based bionano platforms to control mesenchymal stem
cell
differentiation.
PG - 10
LID - 10.1186/s40824-018-0120-3 [doi]
LID - 10
AB - BACKGROUND: In the past decade, stem cells, with their ability to
differentiate into
various types of cells, have been proven to be resourceful in regenerative
medicine
and tissue engineering. Despite the ability to repair damaged parts of organs
and
tissues, the use of stem cells still entails several limitations, such as low
differentiation efficiency and difficulties in guiding differentiation. To

address
these limitations, nanotechnology approaches have been recently implemented
in stem
cell research. It has been discovered that stem cells, in combination with
carbon-based functional materials, show enhanced regenerative performances in
varying biophysical conditions. In particular, several studies have reported

solutions to the conventional quandaries in biomedical engineering, using
synergetic
effects of nanohybrid materials, as well as further development of
technologies to
recover from diverse health conditions such as bone fracture and strokes.
MAIN TEXT:
In this review, we discuss several prior studies regarding the application of
various nanomaterials in controlling the behavior of stem cells. We focus on

the
potential of different types of nanomaterials, such as two-dimensional
materials,
gold nanoparticles, and three-dimensional nanohybrid composites, to control
the
differentiation of human mesenchymal stem cells (hMSCs). These materials have
been
found to affect stem cell functions via the adsorption of
growth/differentiation
factors on the surfaces of nanomaterials and the activation of signaling
pathways
that are mostly related to cell adhesion and differentiation (e.g., FAK,
Smad, Erk,
and Wnt). CONCLUSION: Controlling stem cell differentiation using biophysical
factors, especially the use of nanohybrid materials to functionalize

underlying
substrates wherein the cells attach and grow, is a promising strategy to
achieve
cells of interest in a highly efficient manner. We hope that this review will
facilitate the use of other types of newly discovered and/or synthesized

nanomaterials (e.g., metal transition dichalcogenides, non-toxic quantum
dots, and
metal oxide frameworks) for stem cell-based regenerative therapies.
FAU - Kang, Ee-Seul
AU - Kang ES
AD - 1School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro,
Dongjak-gu, Seoul, 06974 Republic of Korea. ISNI: 0000 0001 0789 9563. GRID:
grid.254224.7
FAU - Kim, Da-Seul
AU - Kim DS
grid.254224.7
FAU - Suhito, Intan Rosalina
AU - Suhito IR
grid.254224.7
FAU - Lee, Wanhee
AU - Lee W
grid.254224.7
FAU - Song, Inbeom
AU - Song I
grid.254224.7
FAU - Kim, Tae-Hyung
AU - Kim TH
grid.254224.7
AD - 2Integrative Research Center for Two-Dimensional Functional Materials,
Institute of
Interdisciplinary Convergence Research, Chung-Ang University, Seoul, 06974
Republic
of Korea. ISNI: 0000 0001 0789 9563. GRID: grid.254224.7
LA - eng
PT - Review
DEP - 20180402
TA - Biomater Res
JT - Biomaterials research
JID - 101650636
PMC - PMC5879765
OTO - NOTNLM
OT - Differentiation
OT - Gold nanoparticles
OT - Graphene
OT - Human mesenchymal stem cell
OT - Three-dimensional graphene composites
OT - Two-dimensional materials
COIS- Not applicable.Not applicable.The authors declare that they have no competing
interests.Springer Nature remains neutral with regard to jurisdictional

claims in
published maps and institutional affiliations.
EDAT- 2018/04/06 06:00
MHDA- 2018/04/06 06:01
CRDT- 2018/04/06 06:00
PHST- 2017/12/28 00:00 [received]
PHST- 2018/03/09 00:00 [accepted]
PHST- 2018/04/06 06:00 [entrez]
PHST- 2018/04/06 06:00 [pubmed]
PHST- 2018/04/06 06:01 [medline]
AID - 120 [pii]
AID - 10.1186/s40824-018-0120-3 [doi]
PST - epublish
SO - Biomater Res. 2018 Apr 2;22:10. doi: 10.1186/s40824-018-0120-3. eCollection
2018.
PMID- 23508571
OWN - NLM
STAT- MEDLINE
DCOM- 20131224
LR - 20181202
IS - 1076-1551 (Print)
IS - 1076-1551 (Linking)
VI - 19
IP - 1
DP - 2013 Apr 30
TI - IL-1β inhibits human osteoblast migration.
PG - 36-42
LID - 10.2119/molmed.2012.00058 [doi]
AB - Bone has a high capacity for self-renewal and repair. Prolonged local
secretion of
interleukin 1β (IL-1β), however, is known to be associated with severe bone
loss and
delayed fracture healing. Since induction of bone resorption by IL-1β may not
sufficiently explain these pathologic processes, we investigated, in vitro,

if and
how IL-1β affects migration of multipotent mesenchymal stromal cells (MSC) or
osteoblasts. We found that homogenous exposure to IL-1β significantly

diminished
both nondirectional migration and site-directed migration toward the
chemotactic
factors platelet-derived growth factor (PDGF)-BB and insulin like growth
factor 1
(IGF-1) in osteoblasts. Exposure to a concentration gradient of IL-1β induced
an
even stronger inhibition of migration and completely abolished the migratory
response of osteoblasts toward PDGF-BB, IGF-1, vascular endothelial growth
factor A
(VEGF-A) and the complement factor C5a. IL-1β induced extracellular signal-
regulated
kinases 1 and 2 (ERK1/2) and c-Jun N-terminal kinases (JNK) activation and
inhibition of these signaling pathways suggested an involvement in the IL-1β
effects
on osteoblast migration. In contrast, basal migration of MSC and their
migratory
activity toward PDGF-BB was found to be unaffected by IL-1β. These results
indicate
that the presence of IL-1β leads to impaired recruitment of osteoblasts which
might
influence early stages of fracture healing and could have pathological
relevance for
bone remodeling in inflammatory bone disease.
FAU - Hengartner, Nina-Emily
AU - Hengartner NE
AD - Orthopedic Department, Division for Biochemistry of Joint and Connective
Tissue
Diseases, University of Ulm, Germany.
FAU - Fiedler, Jörg
AU - Fiedler J
FAU - Ignatius, Anita
AU - Ignatius A
FAU - Brenner, Rolf E
AU - Brenner RE
LA - eng
DEP - 20130430
TA - Mol Med
JT - Molecular medicine (Cambridge, Mass.)
JID - 9501023
RN - 0 (IL1R1 protein, human)
RN - 0 (Interleukin-1beta)
RN - 0 (Proto-Oncogene Proteins c-sis)
RN - 0 (Receptors, Interleukin-1 Type I)
RN - 0 (VEGFA protein, human)
RN - 1B56C968OA (Becaplermin)
RN - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB - IM
MH - Becaplermin
MH - Humans
MH - Insulin-Like Growth Factor I/pharmacology
MH - Interleukin-1beta/*pharmacology
MH - Mesenchymal Stem Cells/drug effects/physiology
MH - Mitogen-Activated Protein Kinases/metabolism
MH - Osteoblasts/*drug effects/physiology
MH - Proto-Oncogene Proteins c-sis/pharmacology
MH - Receptors, Interleukin-1 Type I/metabolism
MH - Recombinant Proteins/pharmacology
MH - Vascular Endothelial Growth Factor A/pharmacology
PMC - PMC3646095
EDAT- 2013/03/20 06:00
MHDA- 2013/12/25 06:00
CRDT- 2013/03/20 06:00
PHST- 2012/02/14 00:00 [received]
PHST- 2013/03/11 00:00 [accepted]
PHST- 2013/03/20 06:00 [entrez]
PHST- 2013/03/20 06:00 [pubmed]
PHST- 2013/12/25 06:00 [medline]
AID - molmed.2012.00058 [pii]
AID - 12_058_hengartner [pii]
AID - 10.2119/molmed.2012.00058 [doi]
PST - epublish
SO - Mol Med. 2013 Apr 30;19(1):36-42. doi: 10.2119/molmed.2012.00058.
PMID- 26886459
OWN - NLM
STAT- MEDLINE
DCOM- 20180604
LR - 20180604
IS - 0271-6798 (Linking)
VI - 38
IP - 1
DP - 2018 Jan
TI - Dynamic Splinting in Children and Adolescents With Stiffness After Knee
Surgery.
PG - 38-43
LID - 10.1097/BPO.0000000000000730 [doi]
AB - PURPOSE: The purpose of this study was to investigate the indications and
outcomes
of dynamic splinting (DS) of the arthrofibrotic knee in the pediatric
population.
METHODS: Seventy-four patients (41 males, 33 females) with postoperative
arthrofibrosis treated with DS after an index knee surgery were reviewed.
Median age
was 13 years (range, 4 to 18 y), and median follow-up was 17 months
(interquartile
range, 10 to 28 mo). Demographics, index surgery procedure, preoperative and
postoperative knee range of motion (ROM) measurements, treatment length and
subsequent need for manipulation under anesthesia (MUA), and surgical lysis
of
adhesions (LOA) were evaluated. A ROM deficit was defined as lack of
extension ≥10
degrees or lack of flexion <130 degrees. Successful improvement of ROM was
defined
as an increase of ≥10 degrees in flexion, extension, or both. There were 23
patients
with flexion deficit only, 17 with extension deficit only, and 34 with
combined
flexion and extension deficits. Wilcoxon signed-rank test was used to assess
median
improvement in ROM. Patients were classified into 4 surgical groups: anterior
cruciate ligament (ACL) reconstruction without meniscal repair (n=19), ACL

reconstruction with meniscal repair (n=12), tibial spine fracture repair
(n=21), and
other (n=22). Multivariable logistic regression was used to identify
independent
predictors of failure of DS requiring MUA and LOA. RESULTS: A total of 57
patients
with flexion deficits showed median improvement of 30 degrees in flexion (95%
confidence interval, 0-90 degrees; P<0.001), and 51 patients with extension

deficits
showed median improvement of 7 degrees in extension (95% confidence interval,
0-60
degrees; P<0.001). DS was associated with ROM improvement in 84% and avoided
the
need for surgery in 58% of all 74 patients included in the study.
Multivariate
analysis of the ACL with meniscus repair subgroup revealed that each 1-month
delay
in DS treatment was associated with a 5-fold increased risk of undergoing a
LOA
(P=0.007). Thirty-six (63%) patients with flexion deficit avoided need for
surgery,
whereas 26 (51%) patients with extension deficits avoided surgery.
CONCLUSIONS: Our
data suggest that DS is an effective method to increase knee ROM and reduce
the need
for subsequent MUA/LOA in the pediatric and adolescent patient with
arthrofibrosis
after an index knee surgery. LEVEL OF EVIDENCE: Level IV-retrospective case
series.
FAU - Pace, James L
AU - Pace JL
AD - Division of Orthopaedic Surgery, Children's Hospital Los Angeles, Los
Angeles, CA.
FAU - Nasreddine, Adam Y
AU - Nasreddine AY
AD - Department of Orthopaedic Surgery.
FAU - Simoni, Michael
AU - Simoni M
AD - Harvard Medical School, Boston, MA.
FAU - Zurakowski, David
AU - Zurakowski D
AD - Surgery.
FAU - Kocher, Mininder S
AU - Kocher MS
AD - Harvard Medical School, Boston, MA.
AD - Division of Sports Medicine, Boston Children's Hospital.
LA - eng
PL - United States
TA - J Pediatr Orthop
JT - Journal of pediatric orthopedics
JID - 8109053
SB - IM
MH - Adolescent
MH - Anterior Cruciate Ligament Reconstruction/adverse effects
MH - Arthroplasty, Replacement, Knee/adverse effects
MH - Child
MH - Child, Preschool
MH - Female
MH - Humans
MH - Joint Diseases/etiology/*therapy
MH - Knee Joint/physiopathology/*surgery
MH - Male
MH - Postoperative Complications/*therapy
MH - Range of Motion, Articular
MH - *Splints
MH - Tibia/*surgery
EDAT- 2016/02/18 06:00
MHDA- 2018/06/05 06:00
CRDT- 2016/02/18 06:00
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2018/06/05 06:00 [medline]
PHST- 2016/02/18 06:00 [entrez]
AID - 10.1097/BPO.0000000000000730 [doi]
PST - ppublish
SO - J Pediatr Orthop. 2018 Jan;38(1):38-43. doi: 10.1097/BPO.0000000000000730.
PMID- 27098172
OWN - NLM
STAT- MEDLINE
DCOM- 20170911
LR - 20181202
VI - 33
IP - 5
DP - 2016 May
TI - Local Injection of Bone Mesenchymal Stem Cells and Fibrin Glue Promotes the
Repair
of Bone Atrophic Nonunion In Vivo.
PG - 824-33
LID - 10.1007/s12325-016-0329-2 [doi]
AB - INTRODUCTION: This study aimed to evaluate the efficacy of local injection of
bone
mesenchymal stem cells (BMSCs) and fibrin glue in the treatment of atrophic
nonunion
in an animal model. METHODS: Thirty-six male Lewis rats were randomly
assigned into
three groups: Group A (control group), Group B (atrophic nonunion group), and
Group C (experimental group). All the rats underwent femoral osteotomy of the
right
hind limb, and stabilized with a custom-designed external fixator. Atrophic
nonunion
of the rats in Group B and C was induced by cauterization of the periosteum
and bone
marrow removal, and repaired by injection of fibrin glue and BMSCs-seeded
fibrin
glue, respectively. The surgically treated femurs were assessed by
radiographic and
histological analysis, and biomechanical test. RESULTS: During the follow-up
period,
the external fixator maintained correct placement and all the femurs retained
normal
positioning. Eight weeks postoperatively, atrophic nonunion was detected in
Group B,
with the presence of fibrous connective tissue in the osteotomy gap. The
femurs in
Group C demonstrated complete bony bridging of the osteotomy gap, with the
formation
of plenty of woven bone. CONCLUSION: The repair of bone atrophic nonunion can
be
promoted through local injection of BMSCs and fibrin glue.
FAU - Hao, Chenguang
AU - Hao C
AD - Department of Orthopaedics, The Second Affiliated Hospital of Harbin Medical
University, Harbin, China.
FAU - Wang, Yanlong
AU - Wang Y
FAU - Shao, Lin
AU - Shao L
FAU - Liu, Jianyu
AU - Liu J
FAU - Chen, Lin
AU - Chen L
FAU - Zhao, Zhenyu
AU - Zhao Z
University, Harbin, China. zhenyuzhao78@yeah.net.
LA - eng
DEP - 20160420
PL - United States
TA - Adv Ther
JT - Advances in therapy
JID - 8611864
SB - T
MH - Animals
MH - *Fractures, Ununited/physiopathology/therapy
MH - Male
MH - Rats
MH - Rats, Inbred Lew
MH - Tissue Engineering/methods
OTO - NOTNLM
OT - *Animal study
OT - *Atrophic nonunion
OT - *Bone mesenchymal stem cells
OT - *External fixator
OT - *Orthopedics
OT - *Tissue engineering
EDAT- 2016/04/22 06:00
MHDA- 2017/09/12 06:00
CRDT- 2016/04/22 06:00
PHST- 2016/02/04 00:00 [received]
PHST- 2016/04/22 06:00 [entrez]
PHST- 2016/04/22 06:00 [pubmed]
PHST- 2017/09/12 06:00 [medline]
AID - 10.1007/s12325-016-0329-2 [pii]
AID - 10.1007/s12325-016-0329-2 [doi]
PST - ppublish
SO - Adv Ther. 2016 May;33(5):824-33. doi: 10.1007/s12325-016-0329-2. Epub 2016
Apr 20.
PMID- 28793652
OWN - NLM
LR - 20201001
IS - 1996-1944 (Print)
IS - 1996-1944 (Linking)
VI - 8
IP - 11
DP - 2015 Nov 6
TI - Enhanced Stability of Calcium Sulfate Scaffolds with 45S5 Bioglass for Bone
Repair.
PG - 7498-7510
LID - 10.3390/ma8115398 [doi]
AB - Calcium sulfate (CaSO₄), as a promising tissue repair material, has been
applied
widely due to its outstanding bioabsorbability and osteoconduction. However,
fast
disintegration, insufficient mechanical strength and poor bioactivity have
limited
its further application. In the study, CaSO₄ scaffolds fabricated by using
selective
laser sintering were improved by adding 45S5 bioglass. The 45S5 bioglass
enhanced
stability significantly due to the bond effect of glassy phase between the
CaSO₄
grains. After immersing for four days in simulated body fluid (SBF), the
specimens
with 45S5 bioglass could still retain its original shape compared as opposed
to
specimens without 45S5 bioglass who experienced disintegration. Meanwhile,
its
compressive strength and fracture toughness increased by 80% and 37%,
respectively.
Furthermore, the apatite layer was formed on the CaSO₄ scaffolds with 45S5
bioglass
in SBF, indicating good bioactivity of the scaffolds. In addition, the
scaffolds
showed good ability to support the osteoblast-like cell adhesion and
proliferation.
FAU - Shuai, Cijun
AU - Shuai C
AD - State Key Laboratory of High Performance Complex Manufacturing, Central South
University, Changsha 410083, China. shuai@csu.edu.cn.

FAU - Zhou, Jianhua
AU - Zhou J
University, Changsha 410083, China. zhouyx@csu.edu.cn.

FAU - Wu, Ping
AU - Wu P
AD - College of Chemistry, Xiangtan University, Xiangtan 411105, China.
wuping401@gmail.com.
FAU - Gao, Chengde
AU - Gao C
University, Changsha 410083, China. gaochengde@csu.edu.cn.

FAU - Feng, Pei
AU - Feng P
University, Changsha 410083, China. fengpei@csu.edu.cn.

FAU - Xiao, Tao
AU - Xiao T
AD - Department of Orthopedics, the Second Xiangya Hospital, Central South
University,
Changsha 410011, China. xiaotaocsu@gmail.com.
FAU - Deng, Youwen
AU - Deng Y
AD - Department of Orthopedics, the Second Xiangya Hospital, Central South
University,
Changsha 410011, China. dengjunjiejj@csu.edu.cn.
FAU - Peng, Shuping
AU - Peng S
AD - School of Basic Medical Science, Central South University, Changsha 410078,
China.
shuping@csu.edu.cn.
AD - Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya
School
of Medicine, Central South University, Changsha 410013, China.
shuping@csu.edu.cn.
LA - eng
DEP - 20151106
TA - Materials (Basel)
JT - Materials (Basel, Switzerland)
JID - 101555929
PMC - PMC5458930
OTO - NOTNLM
OT - 45S5 bioglass
OT - calcium sulfate
OT - scaffolds
OT - selective laser sintering
OT - stability
EDAT- 2015/11/06 00:00
MHDA- 2015/11/06 00:01
CRDT- 2017/08/11 06:00
PHST- 2015/09/01 00:00 [received]
PHST- 2015/10/29 00:00 [revised]
PHST- 2015/11/02 00:00 [accepted]
PHST- 2017/08/11 06:00 [entrez]
PHST- 2015/11/06 00:00 [pubmed]
PHST- 2015/11/06 00:01 [medline]
AID - ma8115398 [pii]
AID - materials-08-05398 [pii]
AID - 10.3390/ma8115398 [doi]
PST - epublish
SO - Materials (Basel). 2015 Nov 6;8(11):7498-7510. doi: 10.3390/ma8115398.
PMID- 23198432
OWN - NLM
STAT- MEDLINE
DCOM- 20131031
LR - 20181202
IS - 1001-5515 (Print)
IS - 1001-5515 (Linking)
VI - 29
IP - 5
DP - 2012 Oct
TI - [Experimental study on application recombinant human bone morphogenetic
protein
2(rhBMP-2)/poly-lactide-co-glycolic acid (PLGA)/fibrin sealant(FS) on repair
of
rabbit radial bone defect].
PG - 903-7
AB - This paper is aimed to investigate the repair of rabbit radial bone defect by
the
recombinant human bone morphogenetic protein 2/poly-lactideco-glycolic acid
microsphere with fibrin sealant (rhBMP-2/PLGA/FS). The radial bone defect
models
were prepared using New Zealand white rabbits, which were randomly divided
into 3
groups, experiment group which were injected with eMP-2/PLGA/FS at bone
defect
location, control group which were injected with FS at bone defect location,
and
blank control group without treatment. The ability of repairing bone defect
was
evaluated with X-ray radiograph. Bone mineral density in the defect regions
was
analysed using the level of ossification. The osteogenetic ability of
repairing bone
defect, the degradation of the material, the morphologic change and the bone
formation were assessed by HE staining and Masson staining. The result showed
that
rhBMP-2/PLGA/FS had overwhelming superiority in the osteogenetic ability and
quality
of bone defect over the control group, and it could promote the repair of
bone
defect and could especially repair the radial bone defect of rabbit well. It
may be
a promising and efficient synthetic bone graft.
FAU - Fan, Zhongkai
AU - Fan Z
AD - Department of Orthopaedics, First Affiliated Hospital, Liaoning Medical
University,
Jinzhou 121000, China. flanz@sohu.com
FAU - Cao, Yang
AU - Cao Y
FAU - Zhang, Zhe
AU - Zhang Z
FAU - Zhang, Mingchao
AU - Zhang M
FAU - Lu, Wei
AU - Lu W
FAU - Tang, Lei
AU - Tang L
FAU - Yao, Qi
AU - Yao Q
FAU - Lu, Gang
AU - Lu G
LA - chi
PL - China
TA - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering =
Shengwu
yixue gongchengxue zazhi
JID - 9426398
RN - 0 (Transforming Growth Factor beta)
RN - 0 (recombinant human bone morphogenetic protein-2)
SB - IM
MH - Animals
MH - Bone Morphogenetic Protein 2/*therapeutic use
MH - Bone Substitutes/*therapeutic use
MH - Female
MH - Fibrin Tissue Adhesive/*therapeutic use
MH - Lactic Acid/*therapeutic use
MH - Male
MH - Microspheres
MH - Polyglycolic Acid/*therapeutic use
MH - Rabbits
MH - Radius Fractures/*therapy
MH - Recombinant Proteins/therapeutic use
MH - Transforming Growth Factor beta/*therapeutic use
EDAT- 2012/12/04 06:00
MHDA- 2013/11/01 06:00
CRDT- 2012/12/04 06:00
PHST- 2012/12/04 06:00 [entrez]
PHST- 2012/12/04 06:00 [pubmed]
PHST- 2013/11/01 06:00 [medline]
PST - ppublish
SO - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2012 Oct;29(5):903-7.
PMID- 24749403
OWN - NLM
STAT- MEDLINE
DCOM- 20140508
LR - 20191210
IS - 1550-7033 (Print)
IS - 1550-7033 (Linking)
VI - 10
IP - 6
DP - 2014 Jun
TI - Biocompatibility and bone-repairing effects: comparison between porous
poly-lactic-co-glycolic acid and nano-hydroxyapatite/poly(lactic acid)
scaffolds.
PG - 1091-104
AB - Copolymer composite scaffolds and bioceramic/polymer composite scaffolds are
two
representative forms of composite scaffolds used for bone tissue engineering.
Studies to compare biocompatibility and bone-repairing effects between these

two
scaffolds are significant for selecting or improving the scaffold for
clinical
application. We prepared two porous scaffolds comprising
poly-lactic-acid/poly-glycolic-acid (PLGA) and poly-lactic-acid/nano-
hydroxyapatite
(nHAP/PLA) respectively, and examined their biocompatibility with human bone
marrow-derived mesenchymal stem cells (hMSCs) through evaluating adhesion,
proliferation and osteogenic differentiation potentials of hMSCs in the
scaffold.
Then, the PLGA scaffold with hMSCs (PM construct) and the nHAP/PLA scaffold
with
hMSCs (HPM construct) were transplanted into the rat calvarial defect areas
to
compare their effects on the bone reconstruction. The results showed that the
nHAP/PLA scaffold was in favor of adhesion, matrix deposition and osteogenic
differentiation of hMSCs. For in vivo transplantation, both HPM and PM
constructs
led to mineralization and osteogenesis in the defect area of rat. However,
the area
grafted with PM construct showed a better formation of mature bone than that
with
HPM construct. In addition, the evaluation of in vitro and in vivo
degradation
indicated that the degradation rate of nHAP/PLA scaffold was much lower than
that of
PLGA scaffold. It is inferred that the lower degradation of nHAP/PLA scaffold
should
result in its inferior bone reconstruction in rat calvaria. Therefore, the
preparation of an ideal composite scaffold for bone tissue engineering should
be
taken into account of the balance between its biocompatibility, degradation
rate,
osteoconductivity and mechanical property.
FAU - Zong, Chen
AU - Zong C
FAU - Qian, Xiaodan
AU - Qian X
FAU - Tang, Zihua
AU - Tang Z
FAU - Hu, Qinghong
AU - Hu Q
FAU - Chen, Jiarong
AU - Chen J
FAU - Gao, Changyou
AU - Gao C
FAU - Tang, Ruikang
AU - Tang R
FAU - Tong, Xiangmin
AU - Tong X
FAU - Wang, Jinfu
AU - Wang J
LA - eng
PT - Evaluation Study
PL - United States
TA - J Biomed Nanotechnol
JT - Journal of biomedical nanotechnology
JID - 101230869
RN - 0 (Polyesters)
RN - 0 (Polymers)
RN - 459TN2L5F5 (poly(lactide))
SB - IM
MH - Animals
MH - Bone Substitutes/*administration & dosage/adverse effects/chemistry
MH - Durapatite/*administration & dosage/adverse effects/chemistry
MH - Lactic Acid/*administration & dosage/adverse effects/chemistry
MH - Male
MH - Polyesters
MH - Polyglycolic Acid/*administration & dosage/adverse effects/chemistry
MH - Polymers/*administration & dosage/adverse effects/chemistry
MH - Porosity
MH - Rats
MH - Skull Fractures/pathology/*therapy
MH - *Tissue Scaffolds
EDAT- 2014/04/23 06:00
MHDA- 2014/05/09 06:00
CRDT- 2014/04/23 06:00
PHST- 2014/04/23 06:00 [entrez]
PHST- 2014/04/23 06:00 [pubmed]
PHST- 2014/05/09 06:00 [medline]
AID - 10.1166/jbn.2014.1696 [doi]
PST - ppublish
SO - J Biomed Nanotechnol. 2014 Jun;10(6):1091-104. doi: 10.1166/jbn.2014.1696.
PMID- 29117211
OWN - NLM
STAT- MEDLINE
DCOM- 20171127
LR - 20181113
IS - 1932-6203 (Linking)
VI - 12
IP - 11
DP - 2017
TI - The treatment of femoral neck fracture using VEGF-loaded nanographene coated
internal fixation screws.
PG - e0187447
LID - 10.1371/journal.pone.0187447 [doi]
LID - e0187447
AB - PURPOSE: Previous studies have proved that vascular endothelial growth factor
(VEGF)
has a dual role in the promotion of new bone formation and blood vessel
repair
during fracture healing. However, how to introduce VEGF to a fracture site
safely
and effectively is still a challenge. This study aimed to prepare a VEGF-
loaded
nanographene coated internal fixation screw and to evaluate its effects in
the
treatment of femoral neck fracture. METHODS: Nanographene coated screws were
prepared by direct liquid-phase exfoliation of the graphite method, and the
surface
characteristics were observed through scanning electron microscopy (SEM).
VEGF was
loaded on nanographene coatings through physical adsorption, and the VEGF
controlled
release was examined by ELISA. Then a canine femoral neck fracture model was
built
to examine both the angiogenic and osteogenic properties of the VEGF-loaded
coated
screws. X-ray, micro-CT-based microangiography, and histopathologic
evaluation were
used to assess the fracture healing progress. RESULTS: The results
demonstrated that
nanographene could load VEGF effectively, and the accumulative release of
VEGF
clearly increased during the entire testing period (9 days) without burst
release.
In canine fracture models, the results of X-ray, microangiography, and
histopathologic examination proved that the speed of fracture healing, new
bone
formation area, and revascularization of the fractured femoral heads in the
VEGF-loaded coated screws groups were significantly higher than in the
control
groups. CONCLUSION: Our study proved that VEGF-loaded nanographene coated
screws
were effective in the treatment of femoral neck fracture and prevention of
avascular
necrosis of femoral head.
FAU - Li, Shuo
AU - Li S
AD - Department of Orthopedics, Fudan University, Shanghai, China.
FAU - Yuan, Hengfeng
AU - Yuan H
FAU - Pan, Jianfeng
AU - Pan J
FAU - Fan, Wenshuai
AU - Fan W
FAU - Zhu, Liang
AU - Zhu L
FAU - Yan, Zuoqin
AU - Yan Z
FAU - Guo, Changan
AU - Guo C
LA - eng
DEP - 20171108
TA - PLoS One
JT - PloS one
JID - 101285081
RN - 0 (Coated Materials, Biocompatible)
RN - 7782-42-5 (Graphite)
SB - IM
EIN - PLoS One. 2018 Jan 9;13(1):e0191143. PMID: 29315343
MH - Animals
MH - *Bone Screws
MH - Coated Materials, Biocompatible/*pharmacology
MH - Dogs
MH - Female
MH - Femoral Neck Fractures/diagnostic imaging/pathology/*surgery
MH - Femur Head/diagnostic imaging/drug effects/pathology
MH - *Fracture Fixation, Internal
MH - Graphite/*pharmacology
MH - Human Umbilical Vein Endothelial Cells/drug effects
MH - Humans
MH - Imaging, Three-Dimensional
MH - Male
MH - Microvessels/diagnostic imaging
MH - Nanoparticles/*chemistry/ultrastructure
MH - Vascular Endothelial Growth Factor A/*pharmacology
PMC - PMC5678728
COIS- Competing Interests: The authors have declared that no competing interests
exist.
EDAT- 2017/11/09 06:00
MHDA- 2017/11/29 06:00
CRDT- 2017/11/09 06:00
PHST- 2016/09/07 00:00 [received]
PHST- 2017/10/19 00:00 [accepted]
PHST- 2017/11/09 06:00 [entrez]
PHST- 2017/11/09 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - PONE-D-16-34589 [pii]
AID - 10.1371/journal.pone.0187447 [doi]
PST - epublish
SO - PLoS One. 2017 Nov 8;12(11):e0187447. doi: 10.1371/journal.pone.0187447.
eCollection
2017.
PMID- 26594970
OWN - NLM
STAT- MEDLINE
DCOM- 20160707
LR - 20181202
IS - 1049-2275 (Linking)
VI - 26
IP - 8
DP - 2015 Nov
TI - Reconstruction of Inferior Orbital Wall Fractures Using Bone Fragments.
PG - 2412-4
LID - 10.1097/SCS.0000000000002090 [doi]
AB - INTRODUCTION: Various materials have been used as implants in orbital floor
fractures. The fractured bone fragments, however, are not usually used
because of
their small size and delicate characteristics. To overcome this limitation,
the
authors used autologous bone fragments combined with fibrin glue and an
absorbable
plate to repair inferior orbital wall fractures. METHODS: Thirty-four
patients with
orbital floor fractures treated in a single center from January 2013 to
September
2014 were prospectively evaluated. Patients' demographic characteristics,
clinical
signs and symptoms, physical examination findings, postoperative
complications, and
preoperative and postoperative computed tomography findings were assessed.
Fracture
repair by a transconjunctival approach in which bone fragments were merged
with
fibrin glue and an absorbable plate was performed in all the patients.
RESULTS:
Postoperative computed tomography showed good orbital fracture reduction and
soft
tissue restoration in all the patients. Five patients developed postoperative
diplopia; however, this symptom resolved spontaneously. Exophthalmometry

showed that
the degree of enophthalmos had improved significantly. CONCLUSION: Based on
the
results of this study, the combination of autologous bone fragments and
absorbable
mesh appears to be a safe and feasible option for the reconstruction of
orbital
floor fractures.
FAU - Kim, Jin Tae
AU - Kim JT
AD - Department of Plastic and Reconstructive Surgery, Ilsan Paik Hospital, Inje
University, Goyang, Republic of Korea.
FAU - Lee, Soo-Hyang
AU - Lee SH
LA - eng
PL - United States
TA - J Craniofac Surg
JT - The Journal of craniofacial surgery
JID - 9010410
SB - D
MH - Adolescent
MH - Adult
MH - *Bone Plates
MH - Bone Transplantation/*methods
MH - Child
MH - Diplopia/diagnostic imaging/etiology/surgery
MH - Enophthalmos/diagnostic imaging/surgery
MH - Female
MH - Humans
MH - Male
MH - Middle Aged
MH - Orbital Fractures/diagnostic imaging/*surgery
MH - Postoperative Complications/diagnostic imaging/surgery
MH - Prospective Studies
MH - Reconstructive Surgical Procedures/*methods
MH - *Surgical Mesh
MH - Young Adult
EDAT- 2015/11/26 06:00
MHDA- 2016/07/09 06:00
CRDT- 2015/11/24 06:00
PHST- 2015/11/24 06:00 [entrez]
PHST- 2015/11/26 06:00 [pubmed]
PHST- 2016/07/09 06:00 [medline]
AID - 00001665-201511000-00040 [pii]
AID - 10.1097/SCS.0000000000002090 [doi]
PST - ppublish
SO - J Craniofac Surg. 2015 Nov;26(8):2412-4. doi: 10.1097/SCS.0000000000002090.
PMID- 22804243
OWN - NLM
STAT- MEDLINE
DCOM- 20130401
LR - 20121018
IS - 0161-3499 (Linking)
VI - 41
IP - 7
DP - 2012 Oct
TI - Osteoprogenitor cell therapy in an equine fracture model.
PG - 773-83
LID - 10.1111/j.1532-950X.2012.01024.x [doi]
AB - OBJECTIVE: To compare the efficacy of osteoprogenitors in fibrin glue to
fibrin glue
alone in bone healing of surgically induced ostectomies of the fourth
metacarpal
bones in an equine model. STUDY DESIGN: Experimental. ANIMALS: Adult horses
(n =
10). METHODS: Segmental ostectomies of the 4th metacarpal bone (MC4) were
performed
bilaterally in 10 horses. There was 1 treatment and 1 control limb in each
horse.
Bone defects were randomly injected with either fibrin glue and
osteoprogenitor
cells or fibrin glue alone. Radiography was performed every week until the
study
endpoint at 12 weeks. After euthanasia, bone healing was evaluated using
radiography
and histology. Analysis of radiographic data was conducted using a linear-
mixed
model. Analysis of histologic data was conducted using a general linear
model.
Statistical significance was set at P < .05. RESULTS: Radiographic grayscale
data as
a measure of bone healing revealed no significant difference between
treatment and
control limbs. Radiographic scoring results also showed that the treatment
effect
was not significant. Histologic analysis was consistent with radiographic
analysis
showing no significant difference between the area of bone present in
treatment and
control limbs. CONCLUSION: Injection of periosteal-derived osteoprogenitors
in a
fibrin glue carrier into surgically created ostectomies of MC4 does not
accelerate
bone healing when compared with fibrin glue alone.
CI - © Copyright 2012 by The American College of Veterinary Surgeons.
FAU - McDuffee, Laurie A
AU - McDuffee LA
AD - Department of Health Management, University of Prince Edward Island,
Charlottetown,
PEI, Canada. lmcduffee@upei.ca
FAU - Pack, LeeAnn
AU - Pack L
FAU - Lores, Marcos
AU - Lores M
FAU - Wright, Glenda M
AU - Wright GM
FAU - Esparza-Gonzalez, Blanca
AU - Esparza-Gonzalez B
FAU - Masaoud, Elmabrok
AU - Masaoud E
LA - eng
GR - Canadian Institutes of Health Research/Canada
DEP - 20120713
PL - United States
TA - Vet Surg
JT - Veterinary surgery : VS
JID - 8113214
SB - IM
MH - Animals
MH - Fibrin Tissue Adhesive/therapeutic use
MH - Fractures, Bone/therapy/*veterinary
MH - Fractures, Ununited/veterinary
MH - Horses/*injuries
MH - Metacarpal Bones/injuries/physiology
MH - Periosteum/*cytology
MH - Stem Cell Transplantation/methods/*veterinary
MH - Stem Cells/cytology/*physiology
EDAT- 2012/07/19 06:00
MHDA- 2013/04/02 06:00
CRDT- 2012/07/19 06:00
PHST- 2012/07/19 06:00 [entrez]
PHST- 2012/07/19 06:00 [pubmed]
PHST- 2013/04/02 06:00 [medline]
AID - 10.1111/j.1532-950X.2012.01024.x [doi]
PST - ppublish
SO - Vet Surg. 2012 Oct;41(7):773-83. doi: 10.1111/j.1532-950X.2012.01024.x. Epub
2012
Jul 13.
PMID- 23665116
OWN - NLM
STAT- MEDLINE
DCOM- 20131017
LR - 20200225
IS - 1742-7061 (Print)
IS - 1742-7061 (Linking)
VI - 9
IP - 8
DP - 2013 Aug
TI - A mechanism for effective cell-seeding in rigid, microporous substrates.
PG - 7977-86
LID - S1742-7061(13)00221-3 [pii]
LID - 10.1016/j.actbio.2013.04.040 [doi]
AB - Seeding cells into porous ceramic substrates has been shown to improve
outcomes in
surgical repair of large bone defects, but the physics underlying cellular
ingress
into such scaffolds remains elusive. This paper demonstrates capillary forces
as a
novel, yet simple, self-loading or self-seeding mechanism for rigid,
microporous
substrates. Capillary forces were found to draw cells through a microporous
network
with interconnections smaller than the diameter of the cells in suspension.
Work
here emphasizes CaP-based bone scaffolds containing both macroporosity
(>100μm) and
microporosity (5-50μm); these have been shown to improve bone formation in
vivo as
compared to their macroporous counterparts and also performed better than
microporous scaffolds containing BMP-2 by some measures of bone regeneration.
We
hypothesize that capillary force driven self-seeding in both macro- and
micropores
may underlie this improvement, and present a mathematical model and
experiments that
support this hypothesis. The cell localization and penetration depth within
these
two-dimensional substrates in vitro depends upon both the cell type (size and
stiffness) and the capillary forces generated by the microstructure.

Additional
experiments showing that cell penetration depth in vitro depends on cell size
and
stiffness suggest that microporosity could be tailored to optimize cell
infiltration
in a cell-specific way. Endogenous cells are also drawn into the microporous
network
in vivo. Results have important implications for design of scaffolds for the
healing
of large bone defects, and for controlled release of drugs in vivo.
reserved.
FAU - Polak, S J
AU - Polak SJ
AD - Department of Bioengineering, University of Illinois at Urbana-Champaign,
1304 West
Springfield Avenue, Urbana, IL 61801, USA. spolak2@illinois.edu
FAU - Rustom, L E
AU - Rustom LE
FAU - Genin, G M
AU - Genin GM
FAU - Talcott, M
AU - Talcott M
FAU - Wagoner Johnson, A J
AU - Wagoner Johnson AJ
LA - eng
GR - U01 EB016422/EB/NIBIB NIH HHS/United States
DEP - 20130509
TA - Acta Biomater
JID - 101233144
SB - IM
MH - Animals
MH - Bone Substitutes/*chemical synthesis
MH - Capillary Action
MH - Cell Adhesion/physiology
MH - Cell Line
MH - Equipment Design
MH - Equipment Failure Analysis
MH - Male
MH - Mandibular Fractures/pathology/*surgery
MH - Miniaturization
MH - Osteoblasts/*cytology/physiology/*transplantation
MH - Porosity
MH - Swine
MH - Tissue Engineering/*instrumentation
PMC - PMC6494621
MID - NIHMS988175
EDAT- 2013/05/15 06:00
MHDA- 2013/10/18 06:00
CRDT- 2013/05/14 06:00
PHST- 2012/12/25 00:00 [received]
PHST- 2013/04/23 00:00 [revised]
PHST- 2013/04/24 00:00 [accepted]
PHST- 2013/05/14 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2013/10/18 06:00 [medline]
AID - S1742-7061(13)00221-3 [pii]
AID - 10.1016/j.actbio.2013.04.040 [doi]
PST - ppublish
SO - Acta Biomater. 2013 Aug;9(8):7977-86. doi: 10.1016/j.actbio.2013.04.040. Epub
2013
May 9.
PMID- 26883948
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR - 20181113
IS - 0957-4530 (Print)
IS - 0957-4530 (Linking)
VI - 27
IP - 4
DP - 2016 Apr
TI - Biocompatibility of artificial bone based on vancomycin loaded mesoporous
silica
nanoparticles and calcium sulfate composites.
PG - 64
LID - 10.1007/s10856-016-5671-z [doi]
LID - 64
AB - The aim of this study was to evaluate the in vitro and in vivo
biocompatibility of
artificial bone based on vancomycin loaded mesoporous silica nanoparticles
and
calcium sulfate composites. In vitro cytotoxicity tests by cholecystokinin
octapeptide (CCK-8) assay showed that the 5%Van-MSN-CaSO4 and Van-CaSO4 bone
cements
were cytocompatible for mouse osteoblastic cell line MC3T3-E1. The
microscopic
observation confirmed that MC3T3-E1cells incubated with Van-CaSO4 group and
5%Van-MSN-CaSO4 group exhibited clear spindle-shaped changes, volume increase
and
maturation, showing that these cements supported adhesion of osteoblastic
cells on
their surfaces. In addition, the measurement of alkaline phosphatase activity
revealed the osteoconductive property of these biomaterials. In order to

assess in
vivo biocompatibility, synthesized cements were implanted into the distal
femur of
twelve adult male and female New Zealand rabbits. After implantation in
artificial
defects of the distal femur, 5%Van-MSN-CaSO4 and Van-CaSO4 bone cements did
not
damage the function of main organs of rabbits. In addition, the Van-MSN-CaSO4
composite allowed complete repair of bone defects with new bone formation 3
months
after implantation. These results show potential application of Van-MSN-CaSO4
composites as bone graft materials for the treatment of open fracture in

human due
to its mechanical, osteoconductive and potential sustained drug release
characteristics and the absence of adverse effects on the body.
FAU - Gu, Jisheng
AU - Gu J
AD - Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji
University
School of Medicine, Yanchang Road 301, Shanghai, 200072, China.
FAU - Wang, Teng
AU - Wang T
AD - The First Clinical Medical College of Nanjing Medical University, Nanjing,
Jiangsu,
210029, China.
FAU - Fan, Guoxin
AU - Fan G
University
FAU - Ma, Junhua
AU - Ma J
AD - The First Clinical Medical College of Nanjing Medical University, Nanjing,
Jiangsu,
210029, China.
FAU - Hu, Wei
AU - Hu W
AD - Department of General Surgery, Changhai Hospital, Second Military Medical
University, Changhai Road 168, Shanghai, 200433, China. huweicj@163.com.
FAU - Cai, Xiaobing
AU - Cai X
University
caixbjn@163.com.
LA - eng
DEP - 20160216
TA - J Mater Sci Mater Med
JT - Journal of materials science. Materials in medicine
JID - 9013087
RN - 6Q205EH1VU (Vancomycin)
RN - 7631-86-9 (Silicon Dioxide)
SB - IM
MH - 3T3 Cells
MH - Animals
MH - Bone Cements/*adverse effects/chemistry
MH - Bone Transplantation
MH - Calcium Sulfate/*adverse effects/chemistry
MH - Cell Survival
MH - Female
MH - Male
MH - Mice
MH - Nanoparticles/*adverse effects/chemistry
MH - Rabbits
MH - Silicon Dioxide/*adverse effects/chemistry
MH - Vancomycin/*adverse effects/chemistry
PMC - PMC4756035
EDAT- 2016/02/18 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/18 06:00
PHST- 2015/09/09 00:00 [received]
PHST- 2016/01/08 00:00 [accepted]
PHST- 2016/02/18 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1007/s10856-016-5671-z [pii]
AID - 5671 [pii]
AID - 10.1007/s10856-016-5671-z [doi]
PST - ppublish
SO - J Mater Sci Mater Med. 2016 Apr;27(4):64. doi: 10.1007/s10856-016-5671-z.
Epub 2016
Feb 16.
PMID- 28636926
OWN - NLM
STAT- MEDLINE
DCOM- 20180417
LR - 20181202
IS - 1742-7061 (Linking)
VI - 59
DP - 2017 Sep 1
TI - Enhanced human bone marrow mesenchymal stromal cell adhesion on scaffolds
promotes
cell survival and bone formation.
PG - 94-107
LID - S1742-7061(17)30381-1 [pii]
LID - 10.1016/j.actbio.2017.06.018 [doi]
AB - In order to induce an efficient bone formation with human bone marrow
mesenchymal
stromal cells (hBMSC) associated to a scaffold, it is crucial to determine
the key
points of the hBMSC action after in vivo transplantation as well as the
appropriate
features of a scaffold. To this aim we compared the hBMSC behavior when
grafted onto
two biomaterials allowing different bone potential in vivo. The cancellous
devitalized Tutoplast®-processed bone (TPB) and the synthetic
hydroxyapatite/β-tricalcium-phosphate (HA/βTCP) which give at 6weeks 100% and
50% of
bone formation respectively. We first showed that hBMSC adhesion is two times
favored on TPB in vitro and in vivo compared to HA/βTCP. Biomaterial

structure
analysis indicated that the better cell adhesion on TPB is associated to its
higher
and smooth open pore architecture as well as its content in collagen. Our
6week time
course analysis, showed using qPCR that only adherent cells are able to
survive in
vivo giving thus an advantage in term of cell number on TPB during the first
4weeks
after graft. We then showed that grafted hBMSC survival is crucial as cells
participate directly to bone formation and play a paracrine action via the
secretion
of hIGF1 and hRANKL which are known to regulate the bone formation and
resorption
pathways respectively. Altogether our results point out the importance of
developing
a smooth and open pore scaffold to optimize hBMSC adhesion and ensure cell
survival
in vivo as it is a prerequisite to potentiate their direct and paracrine
functions.
STATEMENT OF SIGNIFICANCE: Around 10% of skeletal fractures do not heal
correctly
causing nonunion. An approach involving mesenchymal stromal cells (MSC)
associated
with biomaterials emerges as an innovative strategy for bone repair. The
diversity
of scaffolds is a source of heterogeneity for bone formation efficiency. In
order to
better determine the characteristics of a powerful scaffold it is crucial to
understand their relationship with cells after graft. Our results highlight
that a
biomaterial architecture similar to cancellous bone is important to promote
MSC
adhesion and ensure cell survival in vivo. Additionally, we demonstrated that
the
grafted MSC play a direct role coupled to a paracrine effect to enhance bone
formation and that both of those roles are governed by the used scaffold.
reserved.
FAU - Mebarki, Miryam
AU - Mebarki M
AD - IMRB U955-E10, INSERM, Creteil, France; Faculty of Medicine, Paris Est
University,
Creteil, France; Engineering and Cellular Therapy Unit, Etablissement
Français du
Sang, Créteil, France.
FAU - Coquelin, Laura
AU - Coquelin L
University,
Français du
FAU - Layrolle, Pierre
AU - Layrolle P
AD - INSERM U957, Lab. Pathophysiology of Bone Resorption, Faculty of Medicine,
University of Nantes, Nantes, France.
FAU - Battaglia, Séverine
AU - Battaglia S
AD - INSERM U957, Lab. Pathophysiology of Bone Resorption, Faculty of Medicine,
University of Nantes, Nantes, France.
FAU - Tossou, Marine
AU - Tossou M
University,
Français du
FAU - Hernigou, Philippe
AU - Hernigou P
University,
Creteil, France; Orthopaedic Surgery Department, Henri-Mondor AP-HP Hospital,
Creteil, France.
FAU - Rouard, Hélène
AU - Rouard H
University,
Français du
FAU - Chevallier, Nathalie
AU - Chevallier N
University,
Français du
Sang, Créteil, France. Electronic address: nathalie.chevallier@efs.sante.fr.
LA - eng
DEP - 20170619
PL - England
TA - Acta Biomater
JID - 101233144
RN - 0 (Antigens, Differentiation)
RN - 0 (IGF1 protein, human)
RN - 0 (RANK Ligand)
RN - 0 (TNFSF11 protein, human)
RN - 0 (beta-tricalcium phosphate)
SB - IM
MH - Antigens, Differentiation/biosynthesis
MH - Cell Adhesion
MH - Cell Survival
MH - Durapatite/*chemistry
MH - Humans
MH - Insulin-Like Growth Factor I/biosynthesis
MH - Mesenchymal Stem Cells/cytology/*metabolism
MH - *Osteogenesis
MH - Paracrine Communication
MH - RANK Ligand/biosynthesis
OTO - NOTNLM
OT - *Biomaterial
OT - *Cell therapy
OT - *HA/βTCP
OT - *Mesenchymal stem cells
OT - *Tutoplast
EDAT- 2017/06/22 06:00
MHDA- 2018/04/18 06:00
CRDT- 2017/06/22 06:00
PHST- 2017/02/09 00:00 [received]
PHST- 2017/06/08 00:00 [revised]
PHST- 2017/06/13 00:00 [accepted]
PHST- 2017/06/22 06:00 [pubmed]
PHST- 2018/04/18 06:00 [medline]
PHST- 2017/06/22 06:00 [entrez]
AID - S1742-7061(17)30381-1 [pii]
AID - 10.1016/j.actbio.2017.06.018 [doi]
PST - ppublish
SO - Acta Biomater. 2017 Sep 1;59:94-107. doi: 10.1016/j.actbio.2017.06.018. Epub
2017
Jun 19.
PMID- 27062846
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR - 20181202
IS - 1002-1892 (Print)
IS - 1002-1892 (Linking)
VI - 30
IP - 1
DP - 2016 Jan
TI - [EFFECTIVENESS OF MODIFIED OPERATION FOR TREATMENT OF OLD MONTEGGIA
FRACTURE].
PG - 50-3
AB - OBJECTIVE: To investigate the effectiveness of a modified surgical treatment
of old
Monteggia fracture. METHODS: Between March 2006 and December 2013, 40 cases
of old
Monteggia fracture were treated with modified operation. Modified operation
procedure included expanding excision of pedicled forearm fascia flap for
reconstruction of the annular ligament and repair of elbow radial lateral
collateral
ligament complex and extending osteotomy of the ulna, callus replantation,
and
internal fixation with steel plate. There were 26 boys and 14 girls, aged 2-
10 years
with an average age of 4 years. Injury was caused by falling in 24 cases, by
traffic
accident in 8 cases, and by falling from height in 8 cases. The disease
duration was
2-11 months (mean, 4 months). Four patients had combined radial nerve palsy.
RESULTS: Incision healed by first intention after operation, without early
complication of radial nerve palsy, fascial compartment syndrome, or
decreased hand
extensor muscle strength. All the children were followed up 1-5 years (mean,
2.5
years). X-ray films showed fracture healing, and the healing time was 10-20
weeks
(mean, 15 weeks). During follow-up, 3 cases had re-dislocation. Neither hand
dysfunction caused by hand muscle adhesion nor radial head bottleneck shape
change
was found. On the basis of the functional evaluation criteria by Mackay, the
results
were excellent in 32 cases, good in 5 cases, and poor in 3 cases; the
excellent and
good rate was 92.5% at last follow-up. CONCLUSION: The modified surgical
treatment
of old Monteggia fracture is an effective method, with good matching of
humeroradial
joint and without internal fixation of the humeroradial joint.
FAU - Wei, Shizhan
AU - Wei S
FAU - Tang, Yujin
AU - Tang Y
FAU - Peng Weibo
AU - Peng Weibo
FAU - Ban, Huadeng
AU - Ban H
LA - chi
PL - China
TA - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike
zazhi =
Chinese journal of reparative and reconstructive surgery
JID - 9425194
SB - IM
MH - *Bone Plates
MH - Child
MH - Elbow
MH - Elbow Joint/*surgery
MH - Female
MH - Fracture Fixation, Internal
MH - Humans
MH - *Joint Dislocations
MH - Ligaments, Articular/surgery
MH - Male
MH - Monteggia's Fracture/*surgery
MH - Orthopedic Procedures/methods
MH - *Osteotomy
MH - Radius
MH - Radius Fractures
MH - Ulna
MH - Ulna Fractures
MH - Wrist Joint
EDAT- 2016/04/12 06:00
MHDA- 2016/09/16 06:00
CRDT- 2016/04/12 06:00
PHST- 2016/04/12 06:00 [entrez]
PHST- 2016/04/12 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
PST - ppublish
SO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2016 Jan;30(1):50-3.
PMID- 30030175
OWN - NLM
STAT- MEDLINE
DCOM- 20190617
LR - 20190617
IS - 1742-7061 (Linking)
VI - 77
DP - 2018 Sep 1
TI - Effects of locally applied adipose tissue-derived microvascular fragments by
thermoresponsive hydrogel on bone healing.
PG - 201-211
LID - S1742-7061(18)30423-9 [pii]
LID - 10.1016/j.actbio.2018.07.029 [doi]
AB - Insufficient vascularization is a major cause for the development of non-
unions. To
overcome this problem, adipose tissue-derived microvascular fragments (MVF)
may
serve as vascularization units. However, their application into bone defects
needs a
carrier system. Herein, we analyzed whether this is achieved by a
thermoresponsive
hydrogel (TRH). MVF were isolated from CD-1 mice and cultivated after
incorporation
into TRH, while non-incorporated MVF served as controls. Viability of MVF was
assessed immunohistochemically over a 7-day period. Moreover, osteotomies

were
induced in femurs of CD-1 mice. The osteotomy gaps were filled with MVF-
loaded TRH
(TRH + MVF), unloaded TRH (TRH) or no material (control). Bone healing was
evaluated
14 and 35 days postoperatively. MVF incorporated into TRH exhibited less
apoptotic
cells and showed a stable vessel morphology compared to controls. Micro-
computed
tomography revealed a reduced bone volume in TRH + MVF femurs.
Histomorphometry
showed less bone and more fibrous tissue after 35 days in TRH + MVF femurs
compared
to controls. Accordingly, TRH + MVF femurs exhibited a lower osseous bridging
score
and a reduced bending stiffness. Histology and Western blot analysis revealed
an
increased vascularization and CD31 expression, whereas vascular endothelial
growth
factor (VEGF) expression was reduced in TRH + MVF femurs. Furthermore, the
callus of
TRH + MVF femurs showed increased receptor activator of NF-κB ligand
expression and
higher numbers of osteoclasts. These findings indicate that TRH is an
appropriate
carrier system for MVF. Application of TRH + MVF increases the
vascularization of
bone defects. However, this impairs bone healing, most likely due to lower
VEGF
expression during the early course of bone healing. STATEMENT OF
SIGNIFICANCE: In
the present study we analyzed for the first time the in vivo performance of a
thermoresponsive hydrogel (TRH) as a delivery system for bioactive

microvascular
fragments (MVF). We found that TRH represents an appropriate carrier for MVF
as
vascularization units and maintains their viability. Application of MVF-
loaded TRH
impaired bone formation in an established murine model of bone healing,
although
vascularization was improved. This unexpected outcome was most likely due to
a
reduced VEGF expression in the early phase bone healing.
reserved.
FAU - Orth, M
AU - Orth M
AD - Department of Trauma, Hand and Reconstructive Surgery, Saarland University,
66421
Homburg, Germany; Institute for Clinical and Experimental Surgery, Saarland
University, 66421 Homburg, Germany. Electronic address: marcel.orth@uks.eu.
FAU - Altmeyer, M A B
AU - Altmeyer MAB
66421
University, 66421 Homburg, Germany.
FAU - Scheuer, C
AU - Scheuer C
AD - Institute for Clinical and Experimental Surgery, Saarland University, 66421
Homburg,
Germany.
FAU - Braun, B J
AU - Braun BJ
66421
FAU - Holstein, J H
AU - Holstein JH
66421
FAU - Eglin, D
AU - Eglin D
AD - AO Research Institute Davos, Davos, Switzerland.
FAU - D'Este, M
AU - D'Este M
AD - AO Research Institute Davos, Davos, Switzerland.
FAU - Histing, T
AU - Histing T
66421
FAU - Laschke, M W
AU - Laschke MW
Homburg,
Germany.
FAU - Pohlemann, T
AU - Pohlemann T
66421
Homburg, Germany.
FAU - Menger, M D
AU - Menger MD
Homburg,
Germany.
LA - eng
DEP - 20180717
PL - England
TA - Acta Biomater
JID - 101233144
RN - 0 (Hydrogels)
SB - IM
MH - Adipose Tissue/*cytology
MH - Animals
MH - Bony Callus/pathology
MH - Elasticity
MH - Femur/pathology
MH - Hydrogels/*chemistry
MH - Male
MH - Mice
MH - *Microcirculation
MH - Microvessels/*growth & development
MH - Osteoclasts/metabolism
MH - Osteotomy
MH - Shear Strength
MH - Vascular Endothelial Growth Factor A/metabolism
MH - Viscosity
OTO - NOTNLM
OT - *Bone healing
OT - *Microvascular fragments
OT - *Thermoresponsive hydrogel
OT - *VEGF
OT - *Vascularization
EDAT- 2018/07/22 06:00
MHDA- 2019/06/18 06:00
CRDT- 2018/07/22 06:00
PHST- 2018/04/09 00:00 [received]
PHST- 2018/06/13 00:00 [revised]
PHST- 2018/07/16 00:00 [accepted]
PHST- 2018/07/22 06:00 [pubmed]
PHST- 2019/06/18 06:00 [medline]
PHST- 2018/07/22 06:00 [entrez]
AID - S1742-7061(18)30423-9 [pii]
AID - 10.1016/j.actbio.2018.07.029 [doi]
PST - ppublish
SO - Acta Biomater. 2018 Sep 1;77:201-211. doi: 10.1016/j.actbio.2018.07.029. Epub
2018
Jul 17.
PMID- 24588873
OWN - NLM
STAT- MEDLINE
DCOM- 20141117
LR - 20140326
IS - 1944-8244 (Linking)
VI - 6
IP - 6
DP - 2014 Mar 26
TI - Synthesis, mechanical properties, and in vitro biocompatibility with
osteoblasts of
calcium silicate-reduced graphene oxide composites.
PG - 3947-62
LID - 10.1021/am500845x [doi]
AB - Calcium silicate (CaSiO3, CS) ceramics are promising bioactive materials for
bone
tissue engineering, particularly for bone repair. However, the low toughness
of CS
limits its application in load-bearing conditions. Recent findings indicating
the
promising biocompatibility of graphene imply that graphene can be used as an
additive to improve the mechanical properties of composites. Here, we report
a
simple method for the synthesis of calcium silicate/reduced graphene oxide
(CS/rGO)
composites using a hydrothermal approach followed by hot isostatic pressing
(HIP).
Adding rGO to pure CS increased the hardness of the material by ∼40%, the
elastic
modulus by ∼52%, and the fracture toughness by ∼123%. Different toughening
mechanisms were observed including crack bridging, crack branching, crack
deflection, and rGO pull-out, thus increasing the resistance to crack
propagation
and leading to a considerable improvement in the fracture toughness of the
composites. The formation of bone-like apatite on a range of CS/rGO
composites with
rGO weight percentages ranging from 0 to 1.5 has been investigated in
simulated body
fluid (SBF). The presence of a bone-like apatite layer on the composite
surface
after soaking in SBF was demonstrated by X-ray diffraction (XRD) and field
emission
scanning electron microscopy (FESEM). The biocompatibility of the CS/rGO
composites
was characterized using methyl thiazole tetrazolium (MTT) assays in vitro.
The cell
adhesion results showed that human osteoblast cells (hFOB) can adhere to and
develop
on the CS/rGO composites. In addition, the proliferation rate and alkaline
phosphatase (ALP) activity of cells on the CS/rGO composites were improved
compared
with the pure CS ceramics. These results suggest that calcium
silicate/reduced
graphene oxide composites are promising materials for biomedical
applications.
FAU - Mehrali, Mehdi
AU - Mehrali M
AD - Department of Mechanical Engineering and Center of advanced Material,
University of
Malaya , 50603, Kuala Lumpur, Malaysia.
FAU - Moghaddam, Ehsan
AU - Moghaddam E
FAU - Shirazi, Seyed Farid Seyed
AU - Shirazi SF
FAU - Baradaran, Saeid
AU - Baradaran S
FAU - Mehrali, Mohammad
AU - Mehrali M
FAU - Latibari, Sara Tahan
AU - Latibari ST
FAU - Metselaar, Hendrik Simon Cornelis
AU - Metselaar HS
FAU - Kadri, Nahrizul Adib
AU - Kadri NA
FAU - Zandi, Keivan
AU - Zandi K
FAU - Osman, Noor Azuan Abu
AU - Osman NA
LA - eng
DEP - 20140314
PL - United States
JID - 101504991
RN - 0 (Calcium Compounds)
RN - 0 (Oxides)
RN - 0 (Polymers)
RN - 0 (Silicates)
RN - 7782-42-5 (Graphite)
RN - S4255P4G5M (calcium silicate)
SB - IM
MH - Biocompatible Materials/chemical synthesis/*chemistry
MH - Calcium Compounds/*chemistry
MH - Cell Adhesion
MH - Cell Line
MH - Graphite/*chemistry
MH - Humans
MH - Osteoblasts/*cytology
MH - Oxides/chemistry
MH - Polymers/chemical synthesis/chemistry
MH - Silicates/*chemistry
MH - Tissue Engineering/*instrumentation
EDAT- 2014/03/05 06:00
MHDA- 2014/11/18 06:00
CRDT- 2014/03/05 06:00
PHST- 2014/03/05 06:00 [entrez]
PHST- 2014/03/05 06:00 [pubmed]
PHST- 2014/11/18 06:00 [medline]
AID - 10.1021/am500845x [doi]
PST - ppublish
SO - ACS Appl Mater Interfaces. 2014 Mar 26;6(6):3947-62. doi: 10.1021/am500845x.
Epub
2014 Mar 14.
PMID- 33116264
OWN - NLM
STAT- MEDLINE
DCOM- 20210302
LR - 20210302
IS - 2045-2322 (Linking)
VI - 10
IP - 1
DP - 2020 Oct 28
TI - Proteomics of regenerated tissue in response to a titanium implant with a
bioactive
surface in a rat tibial defect model.
PG - 18493
LID - 10.1038/s41598-020-75527-2 [doi]
LID - 18493
AB - Due to their excellent mechanical and biocompatibility properties, titanium-
based
implants are successfully used as biomedical devices. However, when new bone
formation fails for different reasons, impaired fracture healing becomes a
clinical
problem and affects the patient's quality of life. We aimed to design a new
bioactive surface of titanium implants with a synergetic PEG biopolymer-based
composition for gradual delivery of growth factors (FGF2, VEGF, and BMP4)
during
bone healing. The optimal architecture of non-cytotoxic polymeric coatings
deposited
by dip coating under controlled parameters was assessed both in cultured
cells and
in a rat tibial defect model (100% viability). Notably, the titanium adsorbed
polymer matrix induced an improved healing process when compared with the
individual
action of each biomolecules. High-performance mass spectrometry analysis
demonstrated that recovery after a traumatic event is governed by specific
differentially regulated proteins, acting in a coordinated response to the
external
stimulus. Predicted protein interactions shown by STRING analysis were well
organized in hub-based networks related with response to chemical, wound
healing and
response to stress pathways. The proposed functional polymer coatings of the
titanium implants demonstrated the significant improvement of bone healing
process
after injury.
FAU - Boteanu, Raluca M
AU - Boteanu RM
AD - Institute of Cellular Biology and Pathology "N. Simionescu" of the Romanian
Academy,
8, B.P. Hasdeu Street, PO Box 35-14, 050568, Bucharest, Romania.
FAU - Suica, Viorel I
AU - Suica VI
Academy,
FAU - Ivan, Luminita
AU - Ivan L
Academy,
FAU - Safciuc, Florentina
AU - Safciuc F
Academy,
FAU - Uyy, Elena
AU - Uyy E
Academy,
FAU - Dragan, Emanuel
AU - Dragan E
Academy,
FAU - Croitoru, Sorin M
AU - Croitoru SM
AD - Faculty of Engineering and Management of Technological Systems, Politehnica
University of Bucharest, Bucharest, Romania.
FAU - Grumezescu, Valentina
AU - Grumezescu V
AD - National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor
Street,
Magurele, P.O. Box MG-54, 77125, Bucharest, Romania.
FAU - Chiritoiu, Marioara
AU - Chiritoiu M
AD - Institute of Biochemistry of the Romanian Academy, Bucharest, Romania.
FAU - Sima, Livia E
AU - Sima LE
AD - Institute of Biochemistry of the Romanian Academy, Bucharest, Romania.
FAU - Vlagioiu, Constantin
AU - Vlagioiu C
AD - Faculty of Veterinary Medicine, University of Agronomic Sciences and
Veterinary
Medicine of Bucharest, Bucharest, Romania.
FAU - Socol, Gabriel
AU - Socol G
AD - National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor
Street,
Magurele, P.O. Box MG-54, 77125, Bucharest, Romania. gabriel.socol@inflpr.ro.
FAU - Antohe, Felicia
AU - Antohe F
Academy,
felicia.antohe@icbp.ro.
LA - eng
DEP - 20201028
TA - Sci Rep
JID - 101563288
RN - 0 (Actins)
RN - 0 (Biopolymers)
SB - IM
MH - Actins/chemistry
MH - Animals
MH - Biopolymers
MH - Cell Adhesion
MH - Coated Materials, Biocompatible/chemistry
MH - Computational Biology
MH - Male
MH - Mass Spectrometry
MH - Microscopy, Fluorescence
MH - *Prostheses and Implants
MH - Prosthesis Design
MH - Proteomics
MH - Rats
MH - Rats, Wistar
MH - Tibia/*physiopathology
PMC - PMC7595204
EDAT- 2020/10/30 06:00
MHDA- 2021/03/03 06:00
CRDT- 2020/10/29 05:46
PHST- 2020/01/30 00:00 [received]
PHST- 2020/10/07 00:00 [accepted]
PHST- 2020/10/29 05:46 [entrez]
PHST- 2020/10/30 06:00 [pubmed]
PHST- 2021/03/03 06:00 [medline]
AID - 10.1038/s41598-020-75527-2 [pii]
AID - 75527 [pii]
AID - 10.1038/s41598-020-75527-2 [doi]
PST - epublish
SO - Sci Rep. 2020 Oct 28;10(1):18493. doi: 10.1038/s41598-020-75527-2.
PMID- 24579215
OWN - NLM
STAT- MEDLINE
DCOM- 20141029
LR - 20191112
IS - 0147-7447 (Linking)
VI - 36
IP - 12
DP - 2013 Dec
TI - Current state and use of biological adhesives in orthopedic surgery.
PG - 945-56
AB - Bone and tissue adhesives are common and beneficial supplements to standard
methods
of musculoskeletal tissue suture repair. Knowledge and development of
biologically
derived or inspired adhesives useful in orthopedic surgery are rapidly
advancing.
Recent literature demonstrates the increased adjunct or primary use of
biological
adhesives in the repair of musculoskeletal soft tissues, chondral fractures,
and
osteochondral fractures. Adhesives offer more benefits and enhancements to
tissue
healing than current fixation methods afford, including improved
biocompatibility,
resorbability, and non-immunogenicity. Further investigation is required to
determine the extent of the role that these bioadhesives can play in
orthopedic
surgery. The largest group of biologically derived adhesives and sealants is
fibrin
sealants, which include first- and second-generation commercially available
fibrin
sealants, autologous fibrin sealants, and variants. Other groups include
gelatin-resorcin aldehydes, protein-aldehyde systems, collagen-based
adhesives,
polysaccharide- based adhesives, mussel adhesive proteins, and various
biologically
inspired or biomimetic glues. Potential uses include applications in
orthopedic-related blood conservation, arthroplasty, articular cartilage
disorders,
sports medicine, spine surgery, trauma, and tumors. The development of an
adhesive
with universal application is likely unfeasible, given the unique
characteristics of
various musculoskeletal tissues. However, the literature demonstrates the
overall
underuse of adhesives and indicates the rising probability of the development
of a
successful variety of bioadhesives for use in orthopedic surgery. As a result
of
reading this article, physicians should be able to: 1. Describe the
difference
between adhesives and sealants. 2. Recognize fibrin adhesives commonly used
in
practice today and identify other biological adhesives with rising potential.
3.
Analyze how fibrin sealants work relative to fibrin and fibrinogen. 4.
Identify
anatomical areas and techniques in which fibrin sealants are used.
FAU - Shah, Neil V
AU - Shah NV
FAU - Meislin, Robert
AU - Meislin R
LA - eng
PT - Review
PL - United States
TA - Orthopedics
JT - Orthopedics
JID - 7806107
SB - IM
CIN - Orthopedics. 2014 Mar;37(3):147-8. PMID: 24598020
CIN - Orthopedics. 2014 Mar;37(3):148. PMID: 24779083
MH - Humans
MH - *Orthopedic Procedures
MH - Tissue Adhesives/*therapeutic use
EDAT- 2014/03/04 06:00
MHDA- 2014/10/30 06:00
CRDT- 2014/03/04 06:00
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2014/10/30 06:00 [medline]
AID - 10.3928/01477447-20131120-09 [doi]
PST - ppublish
SO - Orthopedics. 2013 Dec;36(12):945-56. doi: 10.3928/01477447-20131120-09.
PMID- 29620150
OWN - NLM
STAT- MEDLINE
DCOM- 20180924
LR - 20181114
IS - 1107-3756 (Print)
IS - 1107-3756 (Linking)
VI - 42
IP - 1
DP - 2018 Jul
TI - Lack of endogenous parathyroid hormone delays fracture healing by inhibiting
vascular endothelial growth factor-mediated angiogenesis.
PG - 171-181
LID - 10.3892/ijmm.2018.3614 [doi]
AB - Intermittent low-dose injections of parathyroid hormone (PTH) have been
reported to
exert bone anabolic effects and to promote fracture healing. As an important
proangiogenic cytokine, vascular endothelial growth factor (VEGF) is secreted
by
bone marrow mesenchymal stem cells (BMSCs) and osteoblasts, and serves a
crucial
regulatory role in the process of vascular development and regeneration. To
investigate whether lack of endogenous PTH causes reduced angiogenic capacity
and
thereby delays the process of fracture healing by downregulating the VEGF
signaling
pathway, a PTH knockout (PTHKO) mouse fracture model was generated. Fracture
healing
was observed using X-ray and micro-computerized tomography. Bone anabolic and
angiogenic markers were analyzed by immunohistochemistry and western blot

analysis.
The expression levels of VEGF and associated signaling pathways in murine
BMSC-derived osteoblasts were measured by quantitative polymerase chain
reaction and
western blot analysis. The expression levels of protein kinase A (PKA),
phosphorylated-serine/threonine protein kinase (pAKT), hypoxia-inducible
factor-1α (HIF1α) and VEGF were significantly decreased in BMSC-derived
osteoblasts
from PTHKO mice. In addition, positive platelet endothelial cell adhesion
molecule
staining was reduced in PTHKO mice, as determined by immunohistochemistry.
The
expression levels of HIF1α, VEGF, runt-related transcription factor 2,
osteocalcin
and alkaline phosphatase were also decreased in PTHKO mice, and fracture
healing was
delayed. In conclusion, lack of endogenous PTH may reduce VEGF expression in
BMSC-derived osteoblasts by downregulating the activity of the
PKA/pAKT/HIF1α/VEGF
pathway, thus affecting endochondral bone formation by causing a reduction in
angiogenesis and osteogenesis, ultimately leading to delayed fracture

healing.
FAU - Ding, Qingfeng
AU - Ding Q
AD - Orthopaedic Department, The First Affiliated Hospital of Nanjing Medical
University,
Nanjing, Jiangsu 210029, P.R. China.
FAU - Sun, Peng
AU - Sun P
University,
FAU - Zhou, Hao
AU - Zhou H
University,
FAU - Wan, Bowen
AU - Wan B
University,
FAU - Yin, Jian
AU - Yin J
University,
FAU - Huang, Yao
AU - Huang Y
University,
FAU - Li, Qingqing
AU - Li Q
University,
FAU - Yin, Guoyong
AU - Yin G
University,
FAU - Fan, Jin
AU - Fan J
University,
LA - eng
DEP - 20180403
TA - Int J Mol Med
JT - International journal of molecular medicine
JID - 9810955
RN - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN - 0 (Proliferating Cell Nuclear Antigen)
RN - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB - IM
MH - Animals
MH - Cyclic AMP-Dependent Protein Kinases/metabolism
MH - Down-Regulation/genetics
MH - Human Umbilical Vein Endothelial Cells/metabolism
MH - Humans
MH - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH - Mice, Knockout
MH - Osteogenesis
MH - Parathyroid Hormone/*deficiency/metabolism
MH - Proliferating Cell Nuclear Antigen/metabolism
MH - Proto-Oncogene Proteins c-akt/metabolism
MH - Vascular Endothelial Growth Factor A/*metabolism
PMC - PMC5979887
EDAT- 2018/04/06 06:00
MHDA- 2018/09/25 06:00
CRDT- 2018/04/06 06:00
PHST- 2016/03/03 00:00 [received]
PHST- 2017/12/19 00:00 [accepted]
PHST- 2018/04/06 06:00 [pubmed]
PHST- 2018/09/25 06:00 [medline]
PHST- 2018/04/06 06:00 [entrez]
AID - ijmm-42-01-0171 [pii]
AID - 10.3892/ijmm.2018.3614 [doi]
PST - ppublish
SO - Int J Mol Med. 2018 Jul;42(1):171-181. doi: 10.3892/ijmm.2018.3614. Epub 2018
Apr 3.
PMID- 28001498
OWN - NLM
STAT- MEDLINE
DCOM- 20171204
LR - 20180308
IS - 0920-5063 (Linking)
VI - 28
IP - 4
DP - 2017 Mar
TI - Evaluation of the osteoconductive potential of poly(propylene
carbonate)/nano-hydroxyapatite composites mimicking the osteogenic niche for
bone
augmentation.
PG - 350-364
LID - 10.1080/09205063.2016.1274624 [doi]
AB - Nano-hydroxyapatite (n-HA) reinforced poly(propylene carbonate) (PPC)
composites
were prepared for bone repair and reconstruction. The effects of
reinforcement on
the morphology, mechanical properties and biological performance of n-HA/PPC
composites were investigated. The surface morphology and mechanical
properties of
the composites were characterized by scanning electron microscopy (SEM) and
universal material testing machine. The analytical data showed that good
incorporation and dispersion of n-HA crystals could be obtained in the PPC
matrix at
a 30:70 weight ratio. With the increase of n-HA content, the tensile strength
increased and the fracture elongation rate decreased. In vitro cell culture
revealed
that the composite was favorable template for cell attachment and growth. In
vivo
implantation in femoral condyle defects of rabbits confirmed that the n-
HA/PPC
composite had good biocompatibility and gradual biodegradability, exhibiting
good
performance in guided bone regeneration. The results demonstrates that the
incorporation of n-HA crystals into PPC matrix provides a practical way to
produce
biodegradable and cost-competitive composites mimicking the osteogenic niche
for
bone augmentation.
FAU - Zou, Qin
AU - Zou Q
AD - a Research Center for Nano-Biomaterials, Analytical & Testing Center ,
Sichuan
University , Chengdu , China.
FAU - Liao, Jianguo
AU - Liao J
AD - b School of Materials Science and Engineering , Henan Polytechnic
University ,
Jiaozuo , China.
FAU - Li, Jidong
AU - Li J
Sichuan
FAU - Li, Yubao
AU - Li Y
Sichuan
LA - eng
DEP - 20161229
PL - England
TA - J Biomater Sci Polym Ed
JT - Journal of biomaterials science. Polymer edition
JID - 9007393
RN - 8D08K3S51E (propylene carbonate)
RN - T75W9911L6 (Propane)
SB - IM
MH - Animals
MH - Bone Substitutes/*chemistry/*pharmacology/toxicity
MH - Cell Line, Tumor
MH - Cell Survival/drug effects
MH - Femur/*drug effects/physiology
MH - Humans
MH - Nanocomposites/*chemistry
MH - Propane/*analogs & derivatives/chemistry
MH - Rabbits
OTO - NOTNLM
OT - *Propylene carbonate
OT - *biodegradation
OT - *bone regeneration
OT - *composite
OT - *nano-hydroxyapatite
EDAT- 2016/12/22 06:00
MHDA- 2017/12/05 06:00
CRDT- 2016/12/22 06:00
PHST- 2016/12/22 06:00 [pubmed]
PHST- 2017/12/05 06:00 [medline]
PHST- 2016/12/22 06:00 [entrez]
AID - 10.1080/09205063.2016.1274624 [doi]
PST - ppublish
SO - J Biomater Sci Polym Ed. 2017 Mar;28(4):350-364. doi:
10.1080/09205063.2016.1274624.
Epub 2016 Dec 29.
PMID- 30131011
OWN - NLM
STAT- MEDLINE
DCOM- 20191001
LR - 20191001
IS - 1022-5536 (Linking)
VI - 26
IP - 3
DP - 2018 May-Aug
TI - Comparison of two alternative wound closure methods for tumor arthroplasty of
the
hip: A frequency matched cohort study.
PG - 2309499018792436
LID - 10.1177/2309499018792436 [doi]
AB - OBJECTIVE: To examine the effect of an alternative wound closure method after
tumor
arthroplasty of the hip compared to routine wound closure with skin staples.
METHOD:
Single center, frequency matched cohort study. We reviewed all patients who
underwent tumor resection and endoprosthetic reconstruction of the proximal
femur
for pathologic fracture due to metastatic bone disease or malignant
hematologic bone
disease at our center between 2010 and 2014. All patients treated with
occlusive
wound closure (OWC), a combination of intradermal suture, Steri-Strips™, and
an
occlusive skin adhesive, during this period ( n = 35), were compared to an
equally
sized frequency matched group of patients having undergone routine wound
closure
with conventional skin staples. RESULTS: Patients with OWC were significantly
faster
to achieve dry wound status and consequently had significantly shorter
administration of antibiotics and hospital stay. Compared to the patients
with
conventional wound closure with staples, their wounds were already dry after
a mean
3.4 days (vs. 6.7 days [95%CI: 3-3.8 vs. 5.5-7.9], p < 0.0001), they received
antibiotics for a mean 4.2 days (vs. 6.8 days [95%CI: 3.7-4.8 vs. 5.5-8.0], p
<
0.0003) and their mean hospital stay was 6.3 days (vs. 8.0 days [95%CI: 5.5-7
vs.
6.8-9.3], p < 0.015). Prolonged wound discharge (PWD) for 7 days or more was
observed in 34% of patients ( n = 12) in the conventional group, whereas this
complication was completely absent ( n = 0) in the investigational group. For

every
three patients treated with OWC, one complication of PWD over 7 days is
avoided
(number needed to treat = 3). CONCLUSION: Compared to conventional staples,
OWC
appears to significantly reduce wound complications, use of antibiotics, and
hospital stay in patients undergoing tumor arthroplasty procedures of the
hip. As
such, it may also contribute to a reduction of the substantially increased
risk for
prosthetic joint infection in this patient population.
FAU - Hettwer, Werner H
AU - Hettwer WH
AD - 1 Department of Orthopedic Surgery Rigshospitalet, University of Copenhagen,
Copenhagen, Denmark.
FAU - Horstmann, Peter F
AU - Horstmann PF
FAU - Wu, Chunsen
AU - Wu C
AD - 2 Institute of Clinical Research, University of Southern Denmark, Odense,
Denmark.
AD - 3 Odense University Hospital, Odense, Denmark.
FAU - Petersen, Michael M
AU - Petersen MM
LA - eng
PL - England
TA - J Orthop Surg (Hong Kong)
JT - Journal of orthopaedic surgery (Hong Kong)
JID - 9440382
SB - IM
MH - Adult
MH - Aged
MH - *Arthroplasty, Replacement, Hip
MH - Cohort Studies
MH - Female
MH - Femoral Neoplasms/*surgery
MH - Humans
MH - Length of Stay
MH - Male
MH - Middle Aged
MH - Surgical Fixation Devices
MH - *Wound Closure Techniques
MH - Wound Healing
OTO - NOTNLM
OT - *bone metastases
OT - *bone tumor resection
OT - *endoprosthesis
OT - *hip replacement
OT - *occlusive wound closure
OT - *skin staples
OT - *wound discharge
EDAT- 2018/08/23 06:00
MHDA- 2019/10/02 06:00
CRDT- 2018/08/23 06:00
PHST- 2018/08/23 06:00 [entrez]
PHST- 2018/08/23 06:00 [pubmed]
PHST- 2019/10/02 06:00 [medline]
AID - 10.1177/2309499018792436 [doi]
PST - ppublish
SO - J Orthop Surg (Hong Kong). 2018 May-Aug;26(3):2309499018792436. doi:
10.1177/2309499018792436.
PMID- 24129725
OWN - NLM
STAT- MEDLINE
DCOM- 20140611
LR - 20191210
IS - 1864-6697 (Linking)
VI - 151
IP - 5
DP - 2013 Oct
TI - [Elastic titanium nails for minimally invasive intramedullary splinting of
metacarpal fractures].
PG - 525-31
LID - 10.1055/s-0033-1350875 [doi]
AB - AIM: The goal in treatment of metacarpal fractures is to restore the normal
function
of the hand. Although a majority of these fractures can be treated non-
operatively,
surgery is recommended for displaced fractures and in case of a patient wish
for
primary stability for practise. A poor clinical outcome is described for
metacarpal
shortening of more than 5 millimeters and for rotational deformity. Whereas
plate
osteosynthesis may lead to soft tissue irritation involving tendon adhesions
and
scar formation, we have used the elastic stable intramedullary nailing [ESIN]
technique using titanium elastic nails (TEN) for intramedullary splinting of

short
metacarpal shaft and neck fractures. METHOD: Within 5 years, ESIN was
performed in
95 patients. The operative technique was evaluated retrospectively concerning
its
functional results and complications. RESULTS: Sixty-three patients were
analysed
after an average follow-up of 14.0 ± 5.2 weeks. The mean DASH-Score was 2.3 ±
3.9
points. We saw one implant out-of-position and three cases of skin irritation
affording TEN shortening. Two superficial wound infections were treated

conservatively. Pseudarthrosis and nail breakage were not observed. After TEN
removal 9 ± 2.4 weeks post implantationem no refracture occurred. CONCLUSION:
Minimally invasive intramedullary stabilisation of short metacarpal shaft and

neck
fractures with a TEN is a safe surgical technique and achieves primary
stability for
practise. It reliably leads to fracture healing and produces excellent
functional
results.
CI - Georg Thieme Verlag KG Stuttgart · New York.
FAU - Müller, M C
AU - Müller MC
AD - Klinik und Poliklinik für Orthopädie und Unfallchirurgie,
Universitätsklinikum Bonn.
FAU - Welle, K
AU - Welle K
FAU - Windemuth, M
AU - Windemuth M
FAU - Burger, C
AU - Burger C
FAU - Pennekamp, P H
AU - Pennekamp PH
LA - ger
TT - Elastischer Titannagel zur minimalinvasiven Osteosynthese von
Mittelhandfrakturen.
DEP - 20131015
PL - Germany
TA - Z Orthop Unfall
JT - Zeitschrift fur Orthopadie und Unfallchirurgie
JID - 101308227
SB - IM
MH - Adolescent
MH - Adult
MH - Aged
MH - *Bone Nails
MH - Female
MH - Fracture Fixation, Intramedullary/*instrumentation/methods
MH - Fractures, Bone/diagnostic imaging/*surgery
MH - Humans
MH - Male
MH - Metacarpal Bones/diagnostic imaging/*injuries/*surgery
MH - Middle Aged
MH - Minimally Invasive Surgical Procedures/*instrumentation/methods
MH - Radiography
MH - Splints
MH - *Titanium
MH - Young Adult
EDAT- 2013/10/17 06:00
MHDA- 2014/06/12 06:00
CRDT- 2013/10/17 06:00
PHST- 2013/10/17 06:00 [entrez]
PHST- 2013/10/17 06:00 [pubmed]
PHST- 2014/06/12 06:00 [medline]
AID - 10.1055/s-0033-1350875 [doi]
PST - ppublish
SO - Z Orthop Unfall. 2013 Oct;151(5):525-31. doi: 10.1055/s-0033-1350875. Epub
2013 Oct
15.
PMID- 28960963
OWN - NLM
STAT- MEDLINE
DCOM- 20180709
LR - 20180709
IS - 1525-7797 (Linking)
VI - 18
IP - 11
DP - 2017 Nov 13
TI - Sr-HA-graft-Poly(γ-benzyl-l-glutamate) Nanocomposite Microcarriers:
Controllable
Sr(2+) Release for Accelerating Osteogenenisis and Bony Nonunion Repair.
PG - 3742-3752
LID - 10.1021/acs.biomac.7b01101 [doi]
AB - The microcarrier system offers an attractive method for cellular
amplification and
phenotype enhancement in the field of bone tissue engineering. However, it
remains a
challenge to fabricate porous microcarriers with osteoinductive activity for
speedy
and high-quality osseointegration in regeneration of serious complication of
bone
fracture, like nonunion. Here, we present a facile method for the first time
manufacture microcarriers with osteogenic effects and properties based on
well
controlled and long-term Sr(2+) release. At first, strontium-substituted
hydroxyapatite was prepared (Sr-HA) and a novel
Sr-HA-graft-poly(γ-benzyl-l-glutamate) (Sr-HA-PBLG) nanocomposite was
synthesized.
Then, the microcarriers with highly interconnected macropores were fabricated
by a
double emulsion method, which allowed cells to adhere and proliferate and
secrete
extracellular matrix. Besides, the microcarriers with a relatively uniform
diameter
of 271.5 ± 45.0 μm are feasible for injection. The Sr-HA-PBLG microcarriers
efficiently promoted osteogenic gene expression in vitro. With injection of
the
Sr-HA-PBLG microcarriers loading adipose derived stem cells (ADSCs) into the
nonunion sites, bone regeneration was observed at 8 weeks after injection in
a mice
model.
FAU - Gao, Long
AU - Gao L
AD - Department of Polymer Materials, Shanghai University , Shanghai 200444,
People's
Republic of China.
FAU - Huang, Zhongyue
AU - Huang Z
AD - Minhang Hospital, Fudan University , 200119, Shanghai, China.
FAU - Yan, Shifeng
AU - Yan S
People's
Republic of China.
FAU - Zhang, Kunxi
AU - Zhang K
People's
Republic of China.
FAU - Xu, Shenghua
AU - Xu S
People's
Republic of China.
FAU - Li, Guifei
AU - Li G
People's
Republic of China.
FAU - Cui, Lei
AU - Cui L
AD - Department of Regenerative Medicine, Tong Ji University School of Medicine ,
Shanghai 200092, People's Republic of China.
AD - Department of Plastic Surgery, Beijing Shijitan Hospital, Capital Medical
University
, Beijing 100038, People's Republic of China.
FAU - Yin, Jingbo
AU - Yin J
AUID- ORCID: 0000-0001-7614-0331
People's
Republic of China.
LA - eng
DEP - 20171010
PL - United States
TA - Biomacromolecules
JT - Biomacromolecules
JID - 100892849
RN - 0 (strontium-containing hydroxyapatite)
RN - 25014-27-1 (poly-gamma-benzyl-L-glutamate)
RN - 25513-46-6 (Polyglutamic Acid)
SB - IM
MH - Animals
MH - Durapatite/*administration & dosage/chemistry
MH - Extracellular Matrix/drug effects
MH - Gene Expression Regulation, Developmental/drug effects
MH - Mice
MH - Nanocomposites/*administration & dosage/chemistry
MH - Polyglutamic Acid/administration & dosage/analogs & derivatives/chemistry
MH - *Tissue Engineering
EDAT- 2017/09/30 06:00
MHDA- 2018/07/10 06:00
CRDT- 2017/09/30 06:00
PHST- 2017/09/30 06:00 [pubmed]
PHST- 2018/07/10 06:00 [medline]
PHST- 2017/09/30 06:00 [entrez]
AID - 10.1021/acs.biomac.7b01101 [doi]
PST - ppublish
SO - Biomacromolecules. 2017 Nov 13;18(11):3742-3752. doi:
10.1021/acs.biomac.7b01101.
Epub 2017 Oct 10.
PMID- 29395468
OWN - NLM
STAT- MEDLINE
DCOM- 20190123
LR - 20190123
IS - 0109-5641 (Linking)
VI - 34
IP - 4
DP - 2018 Apr
TI - Development of a novel dental resin cement incorporating FGF-2-loaded polymer
particles with the ability to promote tissue regeneration.

PG - 641-648
LID - S0109-5641(17)31053-9 [pii]
LID - 10.1016/j.dental.2018.01.007 [doi]
AB - OBJECTIVE: Aiming to achieve bioactive dental resins that promote healing of
surrounding tissues, we developed novel poly(2-hydroxyethyl
methacrylate/trimethylolpropane trimethacrylate) (polyHEMA/TMPT) particles.
These
particles have been reported to be useful as a non-biodegradable carrier for
fibroblast growth factor-2 (FGF-2) in vitro. The aim of this study was to
evaluate
the ability of an adhesive resin incorporating FGF-2-loaded polymer particles
to
promote tissue regeneration in vitro and in vivo. METHODS: Experimental
adhesive
resins were prepared by incorporating FGF-2-loaded polyHEMA/TMPT particles
into a
4-META/MMA-based adhesive resin, and the release profiles of FGF-2 were
evaluated.
The proliferation of osteoblast-like cells in the eluate from cured
experimental
resin was assessed. When the experimental resin was implanted into rat
calvaria
defects, bone regeneration was evaluated by microcomputed tomography and
histological observations. RESULTS: Sustained release of FGF-2 from the
experimental
resin was observed for 14 days. Eluate from the cured experimental resin
significantly promoted the proliferation of osteoblast-like cells.
Significantly
greater bone regeneration was observed using the experimental resin compared
with
the control resin without FGF-2. SIGNIFICANCE: 4-META/MMA-based adhesive
resin
incorporating FGF-2-loaded polymer particles is useful to promote tissue
regeneration, suggesting that its application would be beneficial for root-
end
filling or the repair of fractured roots in cases with severely damaged
periodontal
tissue.
CI - Copyright © 2018 The Academy of Dental Materials. Published by Elsevier Ltd.
All
rights reserved.
FAU - Tsuboi, Ririko
AU - Tsuboi R
AD - Department of Biomaterials Science, Osaka University Graduate School of
Dentistry,
1-8 Yamadaoka, Suita, Osaka 565-0871, Japan; Division for Interdisciplinary
Dentistry, Osaka University Dental Hospital, 1-8 Yamadaoka, Suita, Osaka 565-
0871,
Japan. Electronic address: tsuboi-ririko@dent.osaka-u.ac.jp.
FAU - Sasaki, Jun-Ichi
AU - Sasaki JI
Dentistry,
1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
FAU - Kitagawa, Haruaki
AU - Kitagawa H
Dentistry,
FAU - Yoshimoto, Itsumi
AU - Yoshimoto I
Dentistry,
FAU - Takeshige, Fumio
AU - Takeshige F
AD - Division for Interdisciplinary Dentistry, Osaka University Dental Hospital,
1-8
Yamadaoka, Suita, Osaka 565-0871, Japan.
FAU - Imazato, Satoshi
AU - Imazato S
Dentistry,
LA - eng
DEP - 20180201
PL - England
TA - Dent Mater
JT - Dental materials : official publication of the Academy of Dental Materials
JID - 8508040
RN - 0 (Polymers)
RN - 0 (Resins, Synthetic)
RN - 103107-01-3 (Fibroblast Growth Factor 2)
RN - 6E1I4IV47V (hydroxyethyl methacrylate)
RN - S734UC120F (trimethylolpropane trimethacrylate)
SB - D
MH - Animals
MH - Dental Materials/chemistry/pharmacology
MH - Fibroblast Growth Factor 2/*pharmacology
MH - Methacrylates/chemistry/*pharmacology
MH - Microscopy, Electron, Scanning
MH - Osteoblasts/*drug effects
MH - Particle Size
MH - Polymers
MH - Rats
MH - Resins, Synthetic/chemistry/*pharmacology
MH - Skull/diagnostic imaging/drug effects
OTO - NOTNLM
OT - *Adhesive resin
OT - *Bone
OT - *FGF-2
OT - *Osteoblast-like cell
OT - *Tissue regeneration
OT - *polyHEMA/TMPT particle
EDAT- 2018/02/06 06:00
MHDA- 2019/01/24 06:00
CRDT- 2018/02/04 06:00
PHST- 2017/09/12 00:00 [received]
PHST- 2018/01/10 00:00 [revised]
PHST- 2018/01/10 00:00 [accepted]
PHST- 2018/02/06 06:00 [pubmed]
PHST- 2019/01/24 06:00 [medline]
PHST- 2018/02/04 06:00 [entrez]
AID - S0109-5641(17)31053-9 [pii]
AID - 10.1016/j.dental.2018.01.007 [doi]
PST - ppublish
SO - Dent Mater. 2018 Apr;34(4):641-648. doi: 10.1016/j.dental.2018.01.007. Epub
2018 Feb
1.
PMID- 25231276
OWN - NLM
STAT- MEDLINE
DCOM- 20150217
LR - 20180222
IS - 0736-0266 (Linking)
VI - 33
IP - 1
DP - 2015 Jan
TI - Local manganese chloride treatment accelerates fracture healing in a rat
model.
PG - 122-30
LID - 10.1002/jor.22733 [doi]
AB - This study investigated the effects of local delivery of manganese chloride
(MnCl2),
an insulin-mimetic compound, upon fracture healing using a rat femoral
fracture
model. Mechanical testing, histomorphometry, and immunohistochemistry were
performed
to assess early and late parameters of fracture healing. At 4 weeks post-
fracture,
maximum torque to failure was 70% higher (P<0.05) and maximum torsional
rigidity
increased 133% (P<0.05) in animals treated with 0.125 mg/kg MnCl2 compared to
saline
controls. Histological analysis of the fracture callus revealed percent new
mineralized tissue was 17% higher (P<0.05) at day 10. Immunohistochemical
analysis
of the 0.125 mg/kg MnCl2 treated group, compared to saline controls, showed a
379%
increase in the density of VEGF-C+ cells. In addition, compared to saline
controls,
the 0.125 mg/kg MnCl2 treated group showed a 233% and 150% increase in blood
vessel
density in the subperiosteal region at day 10 post-fracture as assessed by
detection
of PECAM and smooth muscle α actin, respectively. The results suggest that
local
MnCl2 treatment accelerates fracture healing by increasing mechanical
parameters via
a potential mechanism of amplified early angiogenesis leading to increased
osteogenesis. Therefore, local administration of MnCl2 is a potential
therapeutic
adjunct for fracture healing.
FAU - Hreha, Jeremy
AU - Hreha J
AD - Department of Orthopaedics, Rutgers-New Jersey Medical School, 90 Bergen
Street,
Suite 7300, Newark, New Jersey, 07103.
FAU - Wey, Aaron
AU - Wey A
FAU - Cunningham, Catherine
AU - Cunningham C
FAU - Krell, Ethan S
AU - Krell ES
FAU - Brietbart, Eric A
AU - Brietbart EA
FAU - Paglia, David N
AU - Paglia DN
FAU - Montemurro, Nicholas J
AU - Montemurro NJ
FAU - Nguyen, Daniel A
AU - Nguyen DA
FAU - Lee, Yung-Jae
AU - Lee YJ
FAU - Komlos, Daniel
AU - Komlos D
FAU - Lim, Elisha
AU - Lim E
FAU - Benevenia, Joseph
AU - Benevenia J
AU - O'Connor JP
FAU - Lin, Sheldon S
AU - Lin SS
LA - eng
GR - T90 DE021989/DE/NIDCR NIH HHS/United States
DEP - 20140917
PL - United States
TA - J Orthop Res
Research
Society
JID - 8404726
RN - 0 (Actins)
RN - 0 (Chlorides)
RN - 0 (Manganese Compounds)
RN - 0 (smooth muscle actin, rat)
RN - QQE170PANO (manganese chloride)
SB - IM
MH - Actins/metabolism
MH - Animals
MH - Chlorides/*pharmacology/*therapeutic use
MH - Female
MH - Femoral Fractures/*drug therapy/metabolism
MH - Male
MH - Manganese Compounds/*pharmacology/*therapeutic use
MH - Models, Animal
MH - Neovascularization, Physiologic/drug effects
MH - Rats
MH - Rats, Inbred BB
MH - Rats, Wistar
OTO - NOTNLM
OT - bone regeneration
OT - insulin-mimetic
OT - manganese chloride
OT - rat
EDAT- 2014/09/19 06:00
MHDA- 2015/02/18 06:00
CRDT- 2014/09/19 06:00
PHST- 2013/10/01 00:00 [received]
PHST- 2014/08/20 00:00 [accepted]
PHST- 2014/09/19 06:00 [entrez]
PHST- 2014/09/19 06:00 [pubmed]
PHST- 2015/02/18 06:00 [medline]
AID - 10.1002/jor.22733 [doi]
PST - ppublish
SO - J Orthop Res. 2015 Jan;33(1):122-30. doi: 10.1002/jor.22733. Epub 2014 Sep
17.
PMID- 24888232
OWN - NLM
STAT- MEDLINE
DCOM- 20170112
LR - 20170113
IS - 0905-7161 (Linking)
VI - 26
IP - 10
DP - 2015 Oct
TI - Enhanced bone healing by improved fibrin-clot formation via fibrinogen
adsorption on
biphasic calcium phosphate granules.
PG - 1203-10
LID - 10.1111/clr.12431 [doi]
AB - OBJECTIVES: Fibrin clots play an important role in bone tissue regeneration.
This
study aimed at improving the fibrin-clotting rate by coating the surface of
biphasic
calcium phosphate (BCP) granules with fibrinogen (FNG). METHODS AND
MATERIALS: FNG
was coated on the BCP surface using an adsorption and freeze-drying method.
The
surface morphology of FNG-adsorbed BCP (FNG-BCP) was characterized using
scanning
electron microscopy (SEM), and the stability of the adsorbed FNG evaluated by
gel
electrophoresis and circular dichroism (CD) analysis. The biocompatibility of
FNG-BCP was evaluated in vitro using human mesenchymal stem cells, and in
vivo
bone-healing efficiency determined using a rabbit calvarial bone defect
model.
RESULTS: SEM studies showed numerous irregularly distributed FNG fractions
adsorbed
onto the surface of BCP granules. Gel electrophoresis, CD analysis, and in
vitro
coagulation results showed that the adsorbed FGN maintained its native
protein
structure and clotting properties. Biocompatibility experiments showed that
cell
proliferation and adhesion were improved in cells cultivated on the FNG-BCP
granules. After surgical implantation into the bone defects, the FNG-BCP
granules
coagulated at the defect site by reacting with the blood discharged from the
surgical site tissue. In addition, at 8 weeks, the volume of FNG40-BCP
(P = 0.012)
was significantly higher than that of BCP alone in the newly formed bone.
CONCLUSIONS: These results indicate that self-coagulating FNG-CBP granules
may have
the potential to be used as a bone substitute for enabling effective bone
repair
through rapid fibrin-clot formation.
CI - © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Kim, Beom-Su
AU - Kim BS
AD - Wonkwang Bone Regeneration Research Institute, Wonkwang University, Iksan,
Korea.
AD - Bonecell Biotech Inc., Daejeon, Korea.
FAU - Lee, Jun
AU - Lee J
AD - Department of Oral and Maxillofacial Surgery, Daejeon Dental Hospital,
College of
Dentistry, Wonkwang University, Iksan, Korea.
LA - eng
DEP - 20140529
PL - Denmark
TA - Clin Oral Implants Res
JT - Clinical oral implants research
JID - 9105713
RN - 0 (hydroxyapatite-beta tricalcium phosphate)
RN - 9001-32-5 (Fibrinogen)
SB - D
MH - *Adsorption
MH - Animals
MH - Biocompatible Materials/metabolism
MH - *Blood Coagulation
MH - Bone Substitutes/*metabolism
MH - Circular Dichroism
MH - Electrophoresis
MH - Fibrinogen/*metabolism
MH - Hydroxyapatites/*metabolism
MH - Protein Binding
MH - Rabbits
OTO - NOTNLM
OT - biphasic calcium phosphate
OT - bone substitute
OT - fibrin clot
OT - fibrinogen
EDAT- 2014/06/04 06:00
MHDA- 2017/01/14 06:00
CRDT- 2014/06/04 06:00
PHST- 2014/05/03 00:00 [accepted]
PHST- 2014/06/04 06:00 [entrez]
PHST- 2014/06/04 06:00 [pubmed]
PHST- 2017/01/14 06:00 [medline]
AID - 10.1111/clr.12431 [doi]
PST - ppublish
SO - Clin Oral Implants Res. 2015 Oct;26(10):1203-10. doi: 10.1111/clr.12431. Epub
2014
May 29.
PMID- 23172432
OWN - NLM
STAT- MEDLINE
DCOM- 20140109
LR - 20161125
IS - 1049-2275 (Linking)
VI - 23
IP - 6
DP - 2012 Nov
TI - Antiadhesive effect of mixed solution of sodium hyaluronate and sodium
carboxymethylcellulose after blow-out fracture repair.
PG - 1878-83
LID - 10.1097/SCS.0b013e318260efd4 [doi]
AB - Treatment of blow-out fractures is aimed at the prevention of permanent
diplopia and
cosmetically unacceptable enophthalmos. Porous polyethylene sheets are one of
the
most common alloplastic implants for blow-out fracture repair. Because
adhesion
between the porous polyethylene and the orbital soft tissue can result in
restrictions of ocular motility, prevention of postoperative adhesion is
important
in the reconstruction of blow-out fractures. The purpose of this study was to
find
out the effect of the mixed solution of sodium hyaluronate and sodium
carboxymethylcellulose (HACMC) on postoperative adhesion in blow-out fracture
repair
in an animal model.Twenty-four New Zealand white rabbits were used. An 8-mm
defect
was made in the maxillary sinuses including the bone and mucosa. A 10-mm
porous
polyethylene sheet (Medpor; Porex Surgical Inc., Newnan, GA) was inserted in
to the
defect. The rabbits were divided into a control group and a HACMC group. In
the
HACMC group, HACMC solution was instilled onto the surface of the implant and
then
the implant was inserted. The implants were harvested at 1, 2, 4, and 8 weeks
after
surgery (3 implants each period). Hematoxylin and eosin, Masson trichrome,
and CD31
(platelet endothelial cell adhesion molecule-1) stains were performed for
evaluation
of inflammation, fibrosis, and vascularization.Inflammation appeared less
severe in
the HACMC group, but the difference between the 2 groups was not
statistically
significant. The degree of fibrosis was more severe in the control group.
There were
significant differences in the degree of fibrosis between the 2 groups 4 and
8 weeks
after surgery (P = 0.046). The amount of vascularization was similar in both
groups.The HACMC solution seemed to be effective for reducing postoperative
adhesion
in reconstruction of blow-out fractures in a rabbit model. Our results
suggest that
the application of HACMC solution could be an effective adjunct for the
repair of
trap-door fractures or revision of blow-out fractures.
FAU - Lee, Jong Mi
AU - Lee JM
AD - Department of Ophthalmology, Ulsan University Hospital, University of Ulsan
College
of Medicine, Ulsan, Korea.
FAU - Baek, Sehyun
AU - Baek S
LA - eng
PL - United States
JID - 9010410
RN - 0 (Medpor)
RN - 0 (Polyethylenes)
RN - 9004-61-9 (Hyaluronic Acid)
RN - K679OBS311 (Carboxymethylcellulose Sodium)
SB - D
MH - Animals
MH - Carboxymethylcellulose Sodium/*pharmacology
MH - Hyaluronic Acid/*pharmacology
MH - Orbital Fractures/*surgery
MH - Polyethylenes/pharmacology
MH - Postoperative Complications/*prevention & control
MH - Prostheses and Implants
MH - Rabbits
MH - Tissue Adhesions/*prevention & control
EDAT- 2012/11/23 06:00
MHDA- 2014/01/10 06:00
CRDT- 2012/11/23 06:00
PHST- 2012/11/23 06:00 [entrez]
PHST- 2012/11/23 06:00 [pubmed]
PHST- 2014/01/10 06:00 [medline]
AID - 00001665-201211000-00079 [pii]
AID - 10.1097/SCS.0b013e318260efd4 [doi]
PST - ppublish
SO - J Craniofac Surg. 2012 Nov;23(6):1878-83. doi: 10.1097/SCS.0b013e318260efd4.
PMID- 25208584
OWN - NLM
STAT- MEDLINE
DCOM- 20151015
LR - 20181113
VI - 9
DP - 2014 Sep 11
TI - Effect of N-butyl cyanoacrylate on fracture healing in segmental rat tibia
fracture
model.
PG - 76
LID - 10.1186/s13018-014-0076-5 [doi]
LID - 76
AB - BACKGROUND: Comminuted fractures can occur due to severe traumas. The
treatment of
these fractures that may cause serious morbidity and sometimes mortality is
N-butyl
cyanoacrylate. It has been reported that this adhesive provides sufficient
rigid
fixation for bone healing. This study aims to examine cyanoacrylate
radiologically
and histologically to determine whether it provides adequate recovery in
segmental
fractures. The secondary objective is to evaluate N-butyl cyanoacrylate, an
adhesive
material that can hold the fragments on the fracture line together following
reduction. METHODS: Sixteen Sprague-Dawley rats were divided in two groups as
control (n = 8) and experimental (n = 8) groups. In the control group,

segmental
fractures were made and fixated with K-wire. In the experimental group, the
same
surgical procedure was applied and also fragments were stabilized with N-
butyl
cyanoacrylate. RESULTS: On the sixth week, we did not see any statistically
significant difference in the radiological scoring between groups. However,
the
pathological scores of the control group were statistically higher than the
cyanoacrylate group. CONCLUSIONS: We found that cyanoacrylate was rapidly and
easily
applied in the segmental fractures but did not cause any superior
radiological and
clinical results compared to the control group. The cyanoacrylate had low
viscosity,
and it was not capable enough to fill the defects formed between osteotomy
surfaces.
However, it did not adversely affect fracture healing as seen in biopsies
taken as a
result of follow-ups.
FAU - Akcal, Mehmet Akif
AU - Akcal MA
FAU - Poyanli, Oguz
AU - Poyanli O
FAU - Unay, Koray
AU - Unay K
FAU - Esenkaya, Irfan
AU - Esenkaya I
FAU - Gokcen, Bahadir
AU - Gokcen B
FAU - Fıratlıgil, Ayşe Sanem
AU - Fıratlıgil AS
LA - eng
DEP - 20140911
JID - 101265112
RN - F8CEP82QNP (Enbucrilate)
SB - IM
MH - Animals
MH - Enbucrilate/*pharmacology
MH - Fractures, Bone/diagnostic imaging/*drug therapy
MH - Male
MH - Radiography
MH - Tibial Fractures/diagnostic imaging/*drug therapy
PMC - PMC4189668
EDAT- 2014/09/12 06:00
MHDA- 2015/10/16 06:00
CRDT- 2014/09/12 06:00
PHST- 2014/03/11 00:00 [received]
PHST- 2014/07/30 00:00 [accepted]
PHST- 2014/09/12 06:00 [entrez]
PHST- 2014/09/12 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
AID - s13018-014-0076-5 [pii]
AID - 76 [pii]
AID - 10.1186/s13018-014-0076-5 [doi]
PST - epublish
SO - J Orthop Surg Res. 2014 Sep 11;9:76. doi: 10.1186/s13018-014-0076-5.
PMID- 31915363
OWN - NLM
STAT- MEDLINE
DCOM- 20200130
LR - 20200130
IS - 1022-4742 (Linking)
VI - 29
IP - 1
DP - 2020 Jan
TI - Management of a Subgingivally Fractured Maxillary Central Incisor by
Reattachment
Technique.
PG - 228-233
AB - Reattachment of a fractured fragment to the remaining tooth is challenging
but one
of the best treatment protocols in regards to aesthetics, function as well as
patients acceptance. If the original tooth fragment is retained following

fracture,
the natural tooth structures can be reattached using adhesive protocols to
ensure
reliable strength, durability and aesthetics. This case report will discuss a
13
years old boy with a complicated crown-root fracture of maxillary right
central
incisor tooth. The procedure used to repair the fracture regarding this case
including flap surgery with endodontic treatment. The root canal was filled
with a
root canal sealer and gutta-percha. After root canal obturation, fragment was
reattached with an adhesive tooth reattachment technique. After 6 months

evaluation,
clinical and radiographic examinations showed a stable re-attachment, good
aesthetic
and healthy periodontium.
FAU - Uddin, M F
AU - Uddin MF
AD - Dr Md Farid Uddin, Associate Professor, Department of Conservative Dentistry
&
Endodontics, Pioneer Dental College & Hospital, Ka-40/1, Lichu Bagan Road,
Joar
Sahara, Baridhara, Dhaka, Bangladesh; E-mail: faridbdsdr@gmail.com.
FAU - Amin, M R
AU - Amin MR
LA - eng
PT - Case Reports
PL - Bangladesh
TA - Mymensingh Med J
JT - Mymensingh medical journal : MMJ
JID - 9601799
SB - IM
MH - Adolescent
MH - Composite Resins
MH - *Dental Bonding
MH - Humans
MH - Incisor/*injuries
MH - Male
MH - Maxilla
MH - Tooth Fractures/complications/*therapy
EDAT- 2020/01/10 06:00
MHDA- 2020/01/31 06:00
CRDT- 2020/01/10 06:00
PHST- 2020/01/10 06:00 [entrez]
PHST- 2020/01/10 06:00 [pubmed]
PHST- 2020/01/31 06:00 [medline]
PST - ppublish
SO - Mymensingh Med J. 2020 Jan;29(1):228-233.
PMID- 28599578
OWN - NLM
STAT- MEDLINE
DCOM- 20180430
LR - 20181022
IS - 0885-3282 (Linking)
VI - 32
IP - 2
DP - 2017 Aug
TI - Antimicrobial and bone-forming activity of a copper coated implant in a
rabbit
model.
PG - 139-149
LID - 10.1177/0885328217713356 [doi]
AB - Current strategies in implant technology are directed to generate bioactive
implants
that are capable to activate the regenerative potential of the surrounding
tissue.
On the other hand, implant-related infections are a common problem in
orthopaedic
trauma patients. To meet both challenges, i.e. to generate a bone implant
with
regenerative and antimicrobial characteristics, we tested the use of copper
coated
nails for surgical fixation in a rabbit model. Copper acetate was
galvanically
deposited with a copper load of 1 µg/mm(2) onto a porous oxide layer of
Ti6Al4V
nails, which were used for the fixation of a tibia fracture, inoculated with
bacteria. After implantation of the nail the concentration of copper ions did
not
increase in blood which indicates that copper released from the implant was
locally
restricted to the fracture site. After four weeks, analyses of the extracted
implants revealed a distinct antimicrobial effect of copper, because copper
completely prevented both a weak adhesion and firm attachment of biofilm-
forming
bacteria on the titanium implant. To evaluate fracture healing, radiographic
examination demonstrated an increased callus index in animals with copper
coated
nails. This result indicates a stimulated bone formation by releasing copper
ions.
We conclude that the use of implants with a defined load of copper ions
enables both
prevention of bacterial infection and the stimulation of regenerative
processes.
FAU - Prinz, Cornelia
AU - Prinz C
AD - 1 DOT GmbH, Charles-Darwin-Ring 1a, Rostock, Germany.
FAU - Elhensheri, Mohamed
AU - Elhensheri M
AD - 2 Bioserv GmbH, Rostock, Germany.
FAU - Rychly, Joachim
AU - Rychly J
FAU - Neumann, Hans-Georg
AU - Neumann HG
LA - eng
DEP - 20170609
PL - England
TA - J Biomater Appl
JT - Journal of biomaterials applications
JID - 8813912
RN - 0 (Anti-Bacterial Agents)
RN - 12743-70-3 (titanium alloy (TiAl6V4))
RN - 789U1901C5 (Copper)
SB - IM
MH - Animals
MH - Anti-Bacterial Agents/chemistry/*therapeutic use
MH - *Bone Nails/microbiology
MH - Coated Materials, Biocompatible/chemistry/*therapeutic use
MH - Copper/chemistry/*therapeutic use
MH - Female
MH - Rabbits
MH - Staphylococcal Infections/complications/drug therapy/microbiology
MH - Staphylococcus aureus/drug effects
MH - Tibial Fractures/complications/drug therapy/microbiology/*surgery
MH - Titanium/chemistry/*therapeutic use
OTO - NOTNLM
OT - *Antimicrobial
OT - *bacteria
OT - *bone healing
OT - *copper
OT - *implant
EDAT- 2017/06/11 06:00
MHDA- 2018/05/01 06:00
CRDT- 2017/06/11 06:00
PHST- 2017/06/11 06:00 [pubmed]
PHST- 2018/05/01 06:00 [medline]
PHST- 2017/06/11 06:00 [entrez]
AID - 10.1177/0885328217713356 [doi]
PST - ppublish
SO - J Biomater Appl. 2017 Aug;32(2):139-149. doi: 10.1177/0885328217713356. Epub
2017
Jun 9.
PMID- 24435526
OWN - NLM
STAT- MEDLINE
DCOM- 20150512
LR - 20181202
IS - 0957-4530 (Linking)
VI - 25
IP - 4
DP - 2014 Apr
TI - Improvement in angiogenesis and osteogenesis with modified cannulated screws
combined with VEGF/PLGA/fibrin glue in femoral neck fractures.
PG - 1165-72
LID - 10.1007/s10856-013-5138-4 [doi]
AB - Angiogenesis is essential for bone healing. Vascular endothelial growth
factor
(VEGF) is regarded as one of the most potent antigenic cytokines; however,
there
have been very few studies that have previously investigated the effects of
VEGF on
bone healing in a femoral neck fracture model. Thus, the aim of the present
study
was to test both the angiogenic and osteogenic properties of a VEGF/poly-
lactic acid
glycolic acid (PLGA) delivery system for the treatment of femoral neck
fractures.
VEGF/PLGA microspheres were prepared by the double emulsion solvent-
evaporation
method and in vitro VEGF release was quantified by an ELISA assay. Then the
preparation of femoral neck fracture model and internal fixation were
performed, and
the effect of the VEGF/PLGA microspheres on bone healing was determined by X-
ray,
radionuclide bone scanning, and histomorphometric evaluation. The release of
VEGF
from the VEGF/PLGA microspheres was sustained for at least 42 days in vitro,
and
suspension of the delivery system in fibrin glue further slowed this VEGF
release
rate. In dogs, revascularization of the fractured femoral heads was
significantly
improved by a local injection of VEGF/PLGA/fibrin glue, and the quality and
speed of
fracture healing were significantly improved in the Experimental group than
in the
Control group. Our study confirmed that the VEGF/PLGA delivery system offers
good
angiogenic and osteogenic properties for the treatment of canine femoral neck
fractures.
FAU - Zhang, Licheng
AU - Zhang L
AD - Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road,
Beijing, 100853, People's Republic of China.

FAU - Zhang, Lihai
AU - Zhang L
FAU - Lan, Xia
AU - Lan X
FAU - Xu, Meng
AU - Xu M
FAU - Mao, Zhi
AU - Mao Z
FAU - Lv, Houchen
AU - Lv H
FAU - Yao, Qi
AU - Yao Q
FAU - Tang, Peifu
AU - Tang P
LA - eng
DEP - 20140117
PL - United States
TA - J Mater Sci Mater Med
JT - Journal of materials science. Materials in medicine
JID - 9013087
SB - IM
MH - Animals
MH - *Bone Screws
MH - Dogs
MH - Drug Carriers/chemistry
MH - Drug Compounding
MH - Femoral Neck Fractures/pathology/physiopathology/*surgery
MH - *Fibrin Tissue Adhesive
MH - *Lactic Acid/chemistry
MH - Microspheres
MH - Neovascularization, Physiologic/*drug effects
MH - *Polyglycolic Acid/chemistry
MH - Vascular Endothelial Growth Factor A/*administration &
dosage/pharmacokinetics
EDAT- 2014/01/18 06:00
MHDA- 2015/05/13 06:00
CRDT- 2014/01/18 06:00
PHST- 2013/07/30 00:00 [received]
PHST- 2013/12/28 00:00 [accepted]
PHST- 2014/01/18 06:00 [entrez]
PHST- 2014/01/18 06:00 [pubmed]
PHST- 2015/05/13 06:00 [medline]
AID - 10.1007/s10856-013-5138-4 [doi]
PST - ppublish
SO - J Mater Sci Mater Med. 2014 Apr;25(4):1165-72. doi: 10.1007/s10856-013-5138-
4. Epub
2014 Jan 17.
PMID- 30084745
OWN - NLM
STAT- MEDLINE
DCOM- 20200713
LR - 20210702
IS - 0897-7151 (Linking)
VI - 36
IP - 4
DP - 2019 Feb 15
TI - Cerebral Edema and Neurological Recovery after Traumatic Brain Injury Are
Worsened
if Accompanied by a Concomitant Long Bone Fracture.
PG - 609-618
LID - 10.1089/neu.2018.5812 [doi]
AB - Progression of severe traumatic brain injury (TBI) is associated with
worsening
cerebral inflammation, but it is unknown how a concomitant bone fracture (FX)
affects this progression. Enoxaparin (ENX), a low molecular weight heparin

often
used for venous thromboembolic prophylaxis, decreases penumbral leukocyte
(LEU)
mobilization in isolated TBI and improves neurological recovery. We
investigated if
TBI accompanied by an FX worsens LEU-mediated cerebral inflammation and if
ENX
alters this process. CD1 male mice underwent controlled cortical impact (CCI)
or
sham craniotomy with or without an open tibial FX, and received either ENX
(1 mg/kg,
three times/day) or saline for 2 days following injury. Randomization defined
four
groups (Sham, CCI, CCI+FX, CCI+FX+ENX, n = 10/group). Two days after CCI,
neurological recovery was assessed with the Garcia Neurological Test (GNT);
intravital microscopy (LEU rolling and adhesion, microvascular leakage) and
blood
hemoglobin levels were also evaluated. Penumbral cerebral neutrophil
sequestration
(Ly-6G immunohistochemistry [IHC]) were evaluated post-mortem. In vivo LEU
rolling
was greater in CCI+FX (45.2 ± 4.8 LEUs/100 μm/min) than in CCI alone (26.5 ±
3.1,
p = 0.007), and was suppressed by ENX (23.2 ± 5.5, p = 0.003 vs. CCI + FX).
Neurovascular permeability was higher in CCI+FX (71.1 ± 2.9%) than CCI alone
(42.5 ± 2.3, p < 0.001). GNT scores were lower in CCI+FX (15.2 ± 0.2) than in
CCI
alone (16.3 ± 0.3, p < 0.001). Hemoglobin was lowest in the CCI+FX+ENX group,
lower
than in Sham or CCI. IHC demonstrated greatest polymorphonuclear neutrophil
(PMN)
invasion in CCI+FX in uninjured cerebral territories. A concomitant long bone
FX
worsens TBI-induced cerebral LEU mobilization, microvascular leakage, and
cerebral
edema, and impairs neurological recovery at 48 h. ENX suppresses this
progression
but may increase bleeding.
FAU - Suto, Yujin
AU - Suto Y
AD - 1 Division of Traumatology, Surgical Clinical Care and Emergency Surgery,
University
of Pennsylvania Perelman School of Medicine , Philadelphia, Pennsylvania.
AD - 2 Department of Neurosurgery, Center for Brain Injury and Repair, University
of
Pennsylvania Perelman School of Medicine , Philadelphia, Pennsylvania.
FAU - Nagata, Katsuhiro
AU - Nagata K
AD - 3 Department of Emergency and Critical Care Medicine, Tokyo Medical
University
Hachioji Medical Center , Tokyo, Japan.
FAU - Ahmed, Syed M
AU - Ahmed SM
University
of
FAU - Jacovides, Christina L
AU - Jacovides CL
University
of
FAU - Browne, Kevin D
AU - Browne KD
of
FAU - Cognetti, John
AU - Cognetti J
of
FAU - Johnson, Victoria E
AU - Johnson VE
of
FAU - Leone, Ryan
AU - Leone R
University
FAU - Kaplan, Lewis J
AU - Kaplan LJ
University
FAU - Smith, Douglas H
AU - Smith DH
of
FAU - Pascual, Jose L
AU - Pascual JL
University
of
LA - eng
GR - R01 NS092398/NS/NINDS NIH HHS/United States
DEP - 20180907
PL - United States
TA - J Neurotrauma
JT - Journal of neurotrauma
JID - 8811626
RN - 0 (Anticoagulants)
RN - 0 (Enoxaparin)
SB - IM
MH - Animals
MH - Anticoagulants/pharmacology
MH - Brain Edema/*etiology
MH - Brain Injuries, Traumatic/*complications
MH - Enoxaparin/pharmacology
MH - Fractures, Bone/*complications
MH - Male
MH - Mice
MH - Recovery of Function/drug effects/*physiology
OTO - NOTNLM
OT - *ENX
OT - *TBI
OT - *intravital microscopy
OT - *multiple trauma
OT - *tibial FX
EDAT- 2018/08/08 06:00
MHDA- 2020/07/14 06:00
CRDT- 2018/08/08 06:00
PHST- 2018/08/08 06:00 [pubmed]
PHST- 2020/07/14 06:00 [medline]
PHST- 2018/08/08 06:00 [entrez]
AID - 10.1089/neu.2018.5812 [doi]
PST - ppublish
SO - J Neurotrauma. 2019 Feb 15;36(4):609-618. doi: 10.1089/neu.2018.5812. Epub
2018 Sep
7.
PMID- 22451091
OWN - NLM
STAT- MEDLINE
DCOM- 20140710
LR - 20181202
IS - 1932-6254 (Print)
IS - 1932-6254 (Linking)
VI - 7
IP - 10
DP - 2013 Oct
TI - Non-rigid calcium phosphate cement containing hydrogel microbeads and
absorbable
fibres seeded with umbilical cord stem cells for bone engineering.
PG - 777-87
LID - 10.1002/term.1466 [doi]
AB - The need for bone repair has increased as the population ages. Non-rigid
calcium
phosphate scaffolds could provide compliance for micro-motions within the
tissues
and yet have load-supporting strength. The objectives of this study were to:
(a)
develop a non-rigid calcium phosphate cement (CPC) with microbeads and fibre
reinforcement; and (b) investigate human umbilical cord mesenchymal stem cell
(hUCMSC) proliferation, osteodifferentiation and mineralization on non-rigid

CPC for
the first time. Non-rigid CPC was fabricated by adding extra tetracalcium
phosphate
in the traditional CPC and by incorporating chitosan, absorbable fibres and
hydrogel
microbeads. The non-rigid CPC-microbead scaffold possessed a strain-at-
failure of
10.7%, much higher than the traditional CPC's strain of 0.05% which is
typical for
brittle bioceramics. Flexural strength of non-rigid CPC-microbead was 4-fold
that of
rigid CPC-microbead scaffold, while work-of-fracture (toughness) was
increased by
20-fold. The strength of non-rigid CPC-microbead-fibre scaffold matched that
of
cancellous bone. hUCMSCs on non-rigid CPC proliferated from 100 cells/mm(2)
at 1 day
to 600 cells/mm(2) at 8 days. Alkaline phosphatase, osteocalcin and collagen
gene
expressions of hUCMSCs were greatly increased, and the cells synthesized bone
minerals. hUCMSCs on non-rigid CPC-microbead-fibre constructs had higher bone
markers and more mineralization than those on rigid CPC controls. In

conclusion,
this study developed the first non-rigid, in situ-setting calcium
phosphate-microbead-fibre scaffold with a strain-at-failure exceeding 10%.
hUCMSCs
showed excellent proliferation, osteodifferentiation and mineralization on
non-rigid
CPC scaffold. The novel non-rigid CPC-hUCMSC construct with good strength,
high
strain-at-failure and toughness, as well as superior stem cell proliferation,
osteodifferentiation and mineralization, is promising for load-bearing bone

regeneration applications.
CI - Copyright © 2012 John Wiley & Sons, Ltd.
FAU - TheinHan, Wahwah
AU - TheinHan W
AD - Biomaterials and Tissue Engineering Division, Department of Endodontics,
Prosthodontics and Operative Dentistry, University of Maryland Dental School,
Baltimore, MD, USA.

FAU - Weir, Michael D
AU - Weir MD
FAU - Simon, Carl G
AU - Simon CG
FAU - Xu, Hockin H K
AU - Xu HH
LA - eng
DEP - 20120327
TA - J Tissue Eng Regen Med
JT - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN - 0 (Actins)
RN - 0 (Anthraquinones)
RN - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN - 60MEW57T9G (alizarin)
SB - IM
MH - Actins/metabolism
MH - Anthraquinones/metabolism
MH - Bone Cements/*pharmacology
MH - Bone and Bones/drug effects/*physiology
MH - Calcium Phosphates/*pharmacology
MH - Cell Differentiation/drug effects/genetics
MH - Fluorescence
MH - Humans
MH - Hydrogel, Polyethylene Glycol Dimethacrylate/*pharmacology
MH - Mechanical Phenomena
MH - Mesenchymal Stem Cells/*cytology/drug effects/metabolism/ultrastructure
MH - *Microspheres
MH - Stress Fibers/drug effects/metabolism
MH - Umbilical Cord/*cytology
PMC - PMC3641181
MID - NIHMS424493
OTO - NOTNLM
OT - calcium phosphate cement (CPC)
OT - human umbilical cord stem cells
OT - non-rigid scaffold
OT - osteogenic differentiation
OT - strength and strain
EDAT- 2012/03/28 06:00
MHDA- 2014/07/11 06:00
CRDT- 2012/03/28 06:00
PHST- 2011/02/03 00:00 [received]
PHST- 2011/10/04 00:00 [revised]
PHST- 2012/01/05 00:00 [accepted]
PHST- 2012/03/28 06:00 [entrez]
PHST- 2012/03/28 06:00 [pubmed]
PHST- 2014/07/11 06:00 [medline]
AID - 10.1002/term.1466 [doi]
PST - ppublish
SO - J Tissue Eng Regen Med. 2013 Oct;7(10):777-87. doi: 10.1002/term.1466. Epub
2012 Mar
27.
PMID- 29625614
OWN - NLM
STAT- MEDLINE
DCOM- 20180917
LR - 20191210
VI - 13
IP - 1
DP - 2018 Apr 6
TI - A novel implant removal technique by endoscopy.
PG - 74
LID - 10.1186/s13018-018-0783-4 [doi]
LID - 74
AB - BACKGROUND: Routine implant removal after fracture healing remains
controversial.
However, it has been suggested that implant removal should be performed in
cases of
joint impingement, painful scar adhesion, and implant malposition. Entrance
selection is relatively critical in patients with poor soft tissue conditions
or
sloughing coverage. We propose an innovative technique using endoscopy.
METHODS:
Consecutive surgeries of endoscopic implant removal performed between 2005
and 2016
by a single experienced arthroscopic surgeon were included. Overall, 73
patients
were enrolled; 44 were not eligible for inclusion and were excluded from the
study.
RESULTS: Twenty-nine patients, including 32 surgical sites, were included.
Twenty-four plates and 166 screws were removed using this technique. There
were five
complications during the follow-up period (range, 0.5 to 104 months; mean,
8.8),
including one broken screw, one persistent knee joint contracture, and three
wound
dehiscence. There were no infections or neurovascular injuries. CONCLUSION:
Implant
removal using endoscopy is a minimally invasive surgery that ensures that the
screw
axis does not strip, and treats the intra-articular pathology concomitantly.
This
innovative technique may be considered as an alternative to the traditional
open
method in cases with good surgical indications.
FAU - Liu, Chang Heng
AU - Liu CH
AD - Department of Orthopaedic surgery, Chang Gung Memorial Hospital, Linkou
Medical
Center, #5, Fusing Street, Gueishan Township, Taoyuan County, 33305, Taiwan,
Republic of China.
AD - Bone and Joint Research Center, Chang Gung Memorial Hospital, Linkou Medical
Center,
Taoyuan, Taiwan.
FAU - Yeh, Wen Lin
AU - Yeh WL
Medical
Republic of China.
Center,
Taoyuan, Taiwan.
AD - Department of Athletic Training and Health, National Taiwan Sports
University,
Taoyuan, Taiwan.
AD - College of Medicine, Chang Gung University, Taoyuan, Taiwan.
FAU - Tsai, Ping Jui
AU - Tsai PJ
Medical
Republic of China.
Center,
Taoyuan, Taiwan.
FAU - Fan, Kuo Feng
AU - Fan KF
Medical
Republic of China.
Center,
Taoyuan, Taiwan.
FAU - Cheng, Hung Wei
AU - Cheng HW
AD - Department of Athletic Training and Health, National Taiwan Sports
University,
Taoyuan, Taiwan.
FAU - Chen, Jian Ming
AU - Chen JM
Medical
Republic of China. vantchen@gmail.com.
Center,
Taoyuan, Taiwan. vantchen@gmail.com.
AD - College of Medicine, Chang Gung University, Taoyuan, Taiwan.
vantchen@gmail.com.
LA - eng
DEP - 20180406
JID - 101265112
SB - IM
MH - Adult
MH - Aged
MH - Arthroscopy/adverse effects/methods
MH - Bone Plates
MH - Bone Screws
MH - Device Removal/adverse effects/*methods
MH - Endoscopy/adverse effects/*methods
MH - Female
MH - Fracture Fixation, Internal/adverse effects/*instrumentation/methods
MH - Humans
MH - *Internal Fixators/adverse effects
MH - Male
MH - Middle Aged
MH - Minimally Invasive Surgical Procedures/adverse effects/methods
MH - Prosthesis Failure
MH - Young Adult
PMC - PMC5889551
OTO - NOTNLM
OT - Arthroscopy
OT - Endoscopy
OT - Implant removal
OT - Minimally invasive
OT - Surgical technique
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Chang Gung Medical Foundation
Institutional Review Board. IRB No. 201601489B0. No consent was needed from
any
patients involved in this retrospectively reviewed case series study. CONSENT
FOR
PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that
they have
no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral
with
regard to jurisdictional claims in published maps and institutional
affiliations.
EDAT- 2018/04/08 06:00
MHDA- 2018/09/18 06:00
CRDT- 2018/04/08 06:00
PHST- 2018/02/18 00:00 [received]
PHST- 2018/03/24 00:00 [accepted]
PHST- 2018/04/08 06:00 [entrez]
PHST- 2018/04/08 06:00 [pubmed]
PHST- 2018/09/18 06:00 [medline]
AID - 10.1186/s13018-018-0783-4 [pii]
AID - 783 [pii]
AID - 10.1186/s13018-018-0783-4 [doi]
PST - epublish
SO - J Orthop Surg Res. 2018 Apr 6;13(1):74. doi: 10.1186/s13018-018-0783-4.
PMID- 30067116
OWN - NLM
STAT- MEDLINE
DCOM- 20190213
LR - 20190215
IS - 0268-8697 (Linking)
VI - 32
IP - 5
DP - 2018 Oct
TI - Use of cyanoacrylate to prevent cerebrospinal fluid fistulas after cranial
surgery.
PG - 544-547
LID - 10.1080/02688697.2018.1494265 [doi]
AB - PURPOSE: Cerebrospinal fluid (CSF) fistula is one of the most common
complications
encountered after cranial surgeries. In cases where CSF fistula frequently
appears
due to surgical technique, dural sealants are used as auxiliary preparations
to
prevent CSF fistula and provide convenience to the surgeon. MATERIALS AND
METHODS:
Data obtained from 128 number of cases where cyanoacrylate (CA) had been used
for
dural repair to prevent CSF fistula was evaluated, retrospectively. The cases
of
skull base and frontal sinus fractures where the primary repair had not been
carried
out were also included in the study. RESULTS: The mean follow-up of all cases
was
9,7 months. CSF fistula was not encountered in 121 of 128 cases. 4 of the
cases
developed CSF fistula in the early period. 3 of the cases presented with CSF
fistula
in the late period after discharge. No side effects due to hypersensitivity
or
preparation were encountered. CONCLUSION: CA can help dural repair against
the
development of CSF fistula by taking effect quickly. Also, it is a rapid
anti-haemorrhagic agent. It can also be used after posterior fossa surgery,
skull
base surgery where dural repair is difficult, or during sinus repair.
FAU - Asan, Ziya
AU - Asan Z
AD - a Department of Neurosurgery, Faculty of Medicine , Ahi Evran University ,
Kırşehir
, Turkey.
FAU - Kilitci, Asuman
AU - Kilitci A
AD - b Department of Pathology, Faculty of Medicine , Ahi Evran University ,
Kırşehir ,
Turkey.
LA - eng
DEP - 20180801
PL - England
TA - Br J Neurosurg
JT - British journal of neurosurgery
JID - 8800054
SB - IM
MH - Adult
MH - Brain/surgery
MH - Cerebrospinal Fluid Leak/*prevention & control
MH - Cerebrospinal Fluid Rhinorrhea/prevention & control
MH - Cranial Fossa, Posterior/surgery
MH - Cyanoacrylates/adverse effects/*therapeutic use
MH - Dura Mater/surgery
MH - Female
MH - Humans
MH - Male
MH - Middle Aged
MH - Neurosurgical Procedures/adverse effects/*methods
MH - Skull Base/surgery
MH - Tissue Adhesives/adverse effects/*therapeutic use
OTO - NOTNLM
OT - CSF fistula
OT - Cerebrospinal fluid fistula
OT - complication
OT - cyanoacrylate
OT - posterior fossa surgery
EDAT- 2018/08/02 06:00
MHDA- 2019/02/14 06:00
CRDT- 2018/08/02 06:00
PHST- 2018/08/02 06:00 [pubmed]
PHST- 2019/02/14 06:00 [medline]
PHST- 2018/08/02 06:00 [entrez]
AID - 10.1080/02688697.2018.1494265 [doi]
PST - ppublish
SO - Br J Neurosurg. 2018 Oct;32(5):544-547. doi: 10.1080/02688697.2018.1494265.
Epub
2018 Aug 1.
PMID- 25260421
OWN - NLM
STAT- MEDLINE
DCOM- 20150612
LR - 20141013
IS - 0142-9612 (Linking)
VI - 35
IP - 38
DP - 2014 Dec
TI - Enhanced bone tissue regeneration by antibacterial and osteoinductive
silica-HACC-zein composite scaffolds loaded with rhBMP-2.
PG - 10033-45
LID - S0142-9612(14)01007-2 [pii]
AB - Next-generation orthopedic implants with both osteoinductivity and
antibacterial
ability are greatly needed. In the present study, biodegradable rhBMP-2
loaded
zein-based scaffolds with a macroporous structure were synthesized, and SBA-
15
nanoparticles and hydroxypropyltrimethyl ammonium chloride chitosan (HACC)
were
incorporated into the scaffolds to produce an anti-infective composite
scaffold for
delivery of osteogenic factors that facilitate the functional repair of bone
defects. The silica/HACC/zein scaffolds developed here showed bioactivity,
biocompatibility, and effective antibacterial activity. Confocal laser
scanning
microscopy (CLSM) was used to quantitatively measure the bactericidal
efficacy with
respect to bacterial adhesion. Results showed that the sample zein-HACC-S20
exhibited long-lasting antibacterial activity against Escherichia coli and
Staphylococcus aureus up to 5 d. At a low dosage of rhBMP-2 (ca. 80 μg), the
scaffolds released rhBMP-2 protein efficiently at a relatively slow rate,
even after
27 d. An ALP activity and ECM mineralization assay showed that the zein-HACC-
S20
scaffolds exhibited significant early osteogenic differentiation by
generating
enhanced ALP product on day 14 and ECM mineralization on day 21. In a mouse
model of
thigh muscle pouches, zein-S20 and zein-HACC-S20 groups resulted in obvious
bone
formation and gave more extensive mineralization to the implants than silica
free
groups, indicating effective bone induction in vivo. In a rabbit model of
critical-sized radius bone defects (20 mm in length and 5 mm in diameter),
the bone
defects were almost fully repaired and bone marrow cavity recanalization was
detectable by 3D micro-CT technique and histological analysis after 12 weeks.
In
this way, the zein-HACC-S20 scaffolds were proven to significantly promote
the bone
repair. They also demonstrated considerable promise for tissue engineering.
Silica/HACC/zein scaffolds with both antibacterial activity and the ability
to
induce osteogenesis have immense potential in orthopedics and other
biomedical
applications.
FAU - Zhou, Panyu
AU - Zhou P
AD - Department of Emergency, Changhai Hospital, Second Military Medical
University,
Shanghai, China; Department of Orthopedics, Changhai Hospital, Second
Military
Medical University, Shanghai, China.
FAU - Xia, Yan
AU - Xia Y
University,
Shanghai, China.
FAU - Cheng, Xiaosong
AU - Cheng X
University,
Shanghai, China.
FAU - Wang, Panfeng
AU - Wang P
University,
Shanghai, China.
FAU - Xie, Yang
AU - Xie Y
AD - Department of Orthopedics, Changhai Hospital, Second Military Medical
University,
Shanghai, China.
FAU - Xu, Shuogui
AU - Xu S
University,
Shanghai, China; Department of Orthopedics, Changhai Hospital, Second
Military
Medical University, Shanghai, China. Electronic address: shuogui126@126.com.
LA - eng
DEP - 20140926
PL - Netherlands
TA - Biomaterials
JT - Biomaterials
JID - 8100316
RN - 0 (Drug Implants)
RN - 0 (SBA-15)
RN - 01Q9PC255D (Ammonium Chloride)
RN - 7631-86-9 (Silicon Dioxide)
RN - 9010-66-6 (Zein)
SB - IM
MH - Ammonium Chloride/*administration & dosage/chemistry
MH - Animals
MH - Anti-Bacterial Agents/administration & dosage
MH - Bacterial Physiological Phenomena/*drug effects
MH - Bone Morphogenetic Protein 2/*administration & dosage/chemistry
MH - Bone Substitutes/administration & dosage
MH - Chitosan/chemistry
MH - Drug Implants/administration & dosage/chemistry
MH - Humans
MH - Mice
MH - Nanocomposites/*administration & dosage/chemistry
MH - Osteogenesis/drug effects/physiology
MH - Rabbits
MH - Radius Fractures/pathology/*therapy
MH - Recombinant Proteins/administration & dosage
MH - Silicon Dioxide/chemistry
MH - Zein/chemistry
OTO - NOTNLM
OT - Antibacterial
OT - Osteoinductive
OT - Silica-HACC-zein
OT - rhBMP-2
EDAT- 2014/09/28 06:00
MHDA- 2015/06/13 06:00
CRDT- 2014/09/28 06:00
PHST- 2014/08/18 00:00 [received]
PHST- 2014/09/06 00:00 [accepted]
PHST- 2014/09/28 06:00 [entrez]
PHST- 2014/09/28 06:00 [pubmed]
PHST- 2015/06/13 06:00 [medline]
AID - S0142-9612(14)01007-2 [pii]
PST - ppublish
SO - Biomaterials. 2014 Dec;35(38):10033-45. doi:
10.1016/j.biomaterials.2014.09.009.
Epub 2014 Sep 26.
PMID- 29306083
OWN - NLM
STAT- MEDLINE
DCOM- 20190612
LR - 20190613
IS - 1878-0180 (Linking)
VI - 79
DP - 2018 Mar
TI - Fabrication and characterization of highly porous barium titanate based
scaffold
coated by Gel/HA nanocomposite with high piezoelectric coefficient for bone
tissue
engineering applications.
PG - 195-202
LID - S1751-6161(17)30587-8 [pii]
LID - 10.1016/j.jmbbm.2017.12.034 [doi]
AB - It is well established that the piezoelectric effect plays an important
physiological role in bone growth, remodeling and fracture healing. Barium
titanate,
as a well-known piezoelectric ceramic, is especially an attractive material
as a
scaffold for bone tissue engineering applications. In this regard, we tried
to
fabricate a highly porous barium titanate based scaffolds by foam replication
method
and polarize them by applying an external electric field. In order to enhance
the
mechanical and biological properties, polarized/non-polarized scaffolds were
coated
with gelatin and nanostructured HA and characterized for their morphologies,
porosities, piezoelectric and mechanical properties. The results showed that
the
compressive strength and piezoelectric coefficient of porous scaffolds
increased
with the increase of sintering temperature. After being coated with Gel/HA
nanocomposite, the interconnected porous structure and pore size of the
scaffolds
almost remain unchanged while the Gel/nHA-coated scaffolds exhibited enhanced
compressive strength and elastic modulus compared with the uncoated samples.
Also,
the effect of polarizing and coating of optimal scaffolds on adhesion,
viability,
and proliferation of the MG63 osteoblast-like cell line was evaluated by
scanning
electron microscope (SEM) and MTT assay. The cell culture experiments
revealed that
developed scaffolds had good biocompatibility and cells were able to adhere,
proliferate and migrate into pores of the scaffolds. Furthermore, cell
density was
significantly higher in the coated scaffolds at all tested time-points. These
results indicated that highly porous barium titanate scaffolds coated with
Gel/HA
nanocomposite has great potential in tissue engineering applications for bone
tissue
repair and regeneration.
FAU - Ehterami, Arian
AU - Ehterami A
AD - Department of Mechanical and Aerospace Engineering, Science and Research
Branch,
Islamic Azad University, Tehran, Iran.
FAU - Kazemi, Mansure
AU - Kazemi M
AD - Department of Tissue Engineering and Applied Cell Sciences, School of
Advanced
Technologies in Medicine, Tehran University of Medical Sciences, Tehran,
Iran.
FAU - Nazari, Bahareh
AU - Nazari B
AD - Department of Medical Biotechnology, School of Advanced Technologies in
Medicine,
Tehran University of Medical Sciences, Tehran, Iran.
FAU - Saraeian, Payam
AU - Saraeian P
AD - Department of Mechanical Engineering, North Tehran Branch, Islamic Azad
University,
Tehran, Iran.
FAU - Azami, Mahmoud
AU - Azami M
Advanced
Technologies in Medicine, Tehran University of Medical Sciences, Tehran,
Iran.
Electronic address: m-azami@tums.ac.ir.
LA - eng
DEP - 20171230
PL - Netherlands
TA - J Mech Behav Biomed Mater
JT - Journal of the mechanical behavior of biomedical materials
JID - 101322406
RN - 24GP945V5T (Barium)
SB - IM
MH - Barium
MH - Bone and Bones
MH - Nanocomposites/*chemistry
MH - Porosity
OTO - NOTNLM
OT - *BaTiO3
OT - *Barium titanate
OT - *Foam replication method
OT - *Piezoelectric ceramics
OT - *d33
EDAT- 2018/01/07 06:00
MHDA- 2019/06/14 06:00
CRDT- 2018/01/07 06:00
PHST- 2017/11/10 00:00 [received]
PHST- 2017/12/25 00:00 [revised]
PHST- 2017/12/29 00:00 [accepted]
PHST- 2018/01/07 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2018/01/07 06:00 [entrez]
AID - S1751-6161(17)30587-8 [pii]
AID - 10.1016/j.jmbbm.2017.12.034 [doi]
PST - ppublish
SO - J Mech Behav Biomed Mater. 2018 Mar;79:195-202. doi:
10.1016/j.jmbbm.2017.12.034.
Epub 2017 Dec 30.
PMID- 33312462
OWN - NLM
LR - 20201216
IS - 2008-6482 (Print)
IS - 2008-6482 (Linking)
VI - 10
IP - 4
DP - 2019
TI - Morphological and Molecular Analysis of Osteoblasts Differentiated from
Mesenchymal
Stem Cells in Polycaprolactone/Magnesium Oxide/Graphene Oxide Scaffold.
PG - 171-182
AB - BACKGROUND: The loss or dysfunction of bone tissue that observed after bone
tumor
resections and severe nonunion fractures afflicts 200 million people
worldwide. Bone
tissue engineering is a promising approach to repair osteoporotic fractures.
OBJECTIVE: In this paper, polycaprolactone (PCL)/magnesium oxide
(MgO)/graphene
oxide (GO) nanofibrous scaffold was fabricated by electrospining method, and
its
biocompatibility and osteogenic differentiation of adipose-derived
mesenchymal stem
cells (MSCs) on this scaffold were evaluated and compared with pure PCL
nanofibrous
scaffold. METHODS: SEM analysis, DAPI staining and MTT assay were used to
evaluation
biocompatibility of PCL/MgO/GO composite scaffold. In addition by ALP assay
and
proteomic approach, osteostimulatory effect of electrospun composite scaffold
was
investigated and the expression level of osteogenic markers including Runt-
related
transcription factor cbfa1/runx2 (runx2), collagen type I (Col1a1) and
osteopontin
(OPN) in MSCs seeded on PCL/MgO/GO composite scaffold was determined and
compared
with pure PCL scaffold. Then, RT-PCR technique was used to validate the level
expression of these genes. RESULTS: The obtained results showed that

adhesion,
viability and ALP activity of MSCs on PCL/MgO/GO scaffold considerably
enhanced
compared with pure PCL. As well as proteomic and real-time analysis
illustrated the
expression of osteogenic markers including runx2, Col1a1 and OPN increased
(>2-fold)
in cells seeded on PCL/MgO/GO composite scaffold. CONCLUSION: It was
concluded that
MgO and GO nanoparticles could improve the biocompatibility of PCL scaffold
and
enhance the osteogenic differentiation of MSCs.
FAU - Niknam, Z
AU - Niknam Z
AD - Faculty of Paramedical Sciences, Shahid Beheshti University of Medical
Sciences,
Tehran, Iran.
AD - Proteomics Research Center, Shahid Beheshti University of Medical Sciences,
Tehran,
Iran.
FAU - Zali, H
AU - Zali H
Tehran,
Iran.
Advanced
Technologies in Medicine, Shahid Beheshti University of Medical Sciences,
Tehran,
Iran.
FAU - Mansouri, V
AU - Mansouri V
AD - Faculty of Paramedical Sciences, Shahid Beheshti University of Medical
Sciences,
Tehran, Iran.
Tehran,
Iran.
FAU - Rezaei Tavirani, M
AU - Rezaei Tavirani M
Tehran,
Iran.
FAU - Omidi, M
AU - Omidi M
AD - Protein Research Center, Shahid Beheshti University, GC, Tehran, Iran.
LA - eng
TA - Int J Organ Transplant Med
JT - International journal of organ transplantation medicine
JID - 101535773
PMC - PMC7722513
OTO - NOTNLM
OT - Mesenchymal stem cells
OT - Osteoblast
OT - Proteomics
OT - Scaffold
EDAT- 2019/01/01 00:00
MHDA- 2019/01/01 00:01
CRDT- 2020/12/14 10:57
PHST- 2020/12/14 10:57 [entrez]
PHST- 2019/01/01 00:00 [pubmed]
PHST- 2019/01/01 00:01 [medline]
AID - ijotm-10-171 [pii]
PST - ppublish
SO - Int J Organ Transplant Med. 2019;10(4):171-182.
PMID- 28285015
OWN - NLM
STAT- MEDLINE
DCOM- 20180212
LR - 20190115
IS - 8756-3282 (Print)
IS - 1873-2763 (Linking)
VI - 99
DP - 2017 Jun
TI - TNFα contributes to diabetes impaired angiogenesis in fracture healing.
PG - 26-38
LID - S8756-3282(17)30068-6 [pii]
LID - 10.1016/j.bone.2017.02.014 [doi]
AB - Diabetes increases the likelihood of fracture, interferes with fracture
healing and
impairs angiogenesis. The latter may be significant due to the critical
nature of
angiogenesis in fracture healing. Although it is known that diabetes
interferes with
angiogenesis the mechanisms remain poorly defined. We examined fracture
healing in
normoglycemic and streptozotocin-induced diabetic mice and quantified the
degree of
angiogenesis with antibodies to three different vascular markers, CD34, CD31
and
Factor VIII. The role of diabetes-enhanced inflammation was investigated by
treatment of the TNFα-specific inhibitor, pegsunercept starting 10days after
induction of fractures. Diabetes decreased both angiogenesis and VEGFA
expression by
chondrocytes. The reduced angiogenesis and VEGFA expression in diabetic
fractures
was rescued by specific inhibition of TNF in vivo. In addition, the TNF
inhibitor
rescued the negative effect of diabetes on endothelial cell proliferation and
endothelial cell apoptosis. The effect of TNFα in vitro was enhanced by high
glucose
and an advanced glycation endproduct to impair microvascular endothelial cell
proliferation and tube formation and to stimulate apoptosis. The effect of

TNF, high
glucose and an AGE was mediated by the transcription factor FOXO1, which
increased
expression of p21 and caspase-3. These studies indicate that inflammation
plays a
major role in diabetes-impaired angiogenesis in endochondral bone formation
through
its effect on microvascular endothelial cells and FOXO1.
FAU - Lim, Jason C
AU - Lim JC
Pennsylvania,
Philadelphia, PA 19104, USA.
FAU - Ko, Kang I
AU - Ko KI
Pennsylvania,
FAU - Mattos, Marcelo
AU - Mattos M
Pennsylvania,
FAU - Fang, Miao
AU - Fang M
AD - Department of Endocrinology, Shanxi Province People's Hospital, Shanxi
Province,
China.
FAU - Zhang, Citong
AU - Zhang C
Pennsylvania,
Philadelphia, PA 19104, USA; Department of Implantology, School of
Stomatology,
Jilin University, Changchun 130021, China.
FAU - Feinberg, Daniel
AU - Feinberg D
Pennsylvania,
FAU - Sindi, Hisham
AU - Sindi H
Pennsylvania,
FAU - Li, Shuai
AU - Li S
AD - Department of Implant Dentistry, Peking University, School and Hospital of
Stomatology, Beijing, China.
FAU - Alblowi, Jazia
AU - Alblowi J
AD - Department of Oral Basic and Clinical Sciences, Faculty of Dentistry, King
Abdulaziz
University, Jeddah, Saudi Arabia.
FAU - Kayal, Rayyan A
AU - Kayal RA
AD - Department of Oral Basic and Clinical Sciences, Faculty of Dentistry, King
Abdulaziz
University, Jeddah, Saudi Arabia.
FAU - Einhorn, Thomas A
AU - Einhorn TA
AD - Department of Orthopedic Surgery, School of Medicine, Boston University,
Boston, MA
02118, USA.
FAU - Gerstenfeld, Louis C
AU - Gerstenfeld LC
AD - Department of Orthopedic Surgery, School of Medicine, Boston University,
Boston, MA
02118, USA.
FAU - Graves, Dana T
AU - Graves DT
Pennsylvania,
Philadelphia, PA 19104, USA. Electronic address: dtgraves@upenn.edu.
LA - eng
GR - P30 AR069619/AR/NIAMS NIH HHS/United States
DEP - 20170308
TA - Bone
JT - Bone
JID - 8504048
RN - 0 (Antigens, CD34)
RN - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN - 0 (Tumor Necrosis Factor-alpha)
RN - 3WJQ0SDW1A (Polyethylene Glycols)
RN - 9001-27-8 (Factor VIII)
RN - Q5I7SFZ853 (PEGylated soluble tumor necrosis factor receptor I)
SB - IM
MH - Animals
MH - Antigens, CD34/blood
MH - Diabetes Mellitus, Experimental/blood/immunology/*metabolism/physiopathology
MH - Factor VIII/metabolism
MH - Fracture Healing/*drug effects/immunology
MH - Hyperglycemia/blood/immunology/metabolism/physiopathology
MH - Inflammation/blood/immunology/metabolism/physiopathology
MH - Male
MH - Mice
MH - Platelet Endothelial Cell Adhesion Molecule-1/blood
MH - Polyethylene Glycols/pharmacology
MH - Receptors, Tumor Necrosis Factor, Type I/pharmacology
MH - Tumor Necrosis Factor-alpha/antagonists & inhibitors/blood/*metabolism
MH - Vascular Endothelial Growth Factor A/blood
PMC - PMC5563392
MID - NIHMS863011
OTO - NOTNLM
OT - *Blood vessel
OT - *Bone
OT - *Cytokine
OT - *Endothelial
OT - *Forkhead
OT - *Hyperglycemia
OT - *Inflammation
OT - *VEGF
OT - *Vascularization
COIS- Disclosures All authors state that they have no conflicts of interest.
EDAT- 2017/03/13 06:00
MHDA- 2018/02/13 06:00
CRDT- 2017/03/13 06:00
PHST- 2016/06/17 00:00 [received]
PHST- 2017/01/05 00:00 [revised]
PHST- 2017/02/26 00:00 [accepted]
PHST- 2017/03/13 06:00 [pubmed]
PHST- 2018/02/13 06:00 [medline]
PHST- 2017/03/13 06:00 [entrez]
AID - S8756-3282(17)30068-6 [pii]
AID - 10.1016/j.bone.2017.02.014 [doi]
PST - ppublish
SO - Bone. 2017 Jun;99:26-38. doi: 10.1016/j.bone.2017.02.014. Epub 2017 Mar 8.
PMID- 32897447
OWN - NLM
STAT- In-Process
LR - 20210205
IS - 1387-2176 (Linking)
VI - 22
IP - 4
DP - 2020 Sep 8
TI - Carbon nanotube-collagen@hydroxyapatite composites with improved mechanical
and
biological properties fabricated by a multi in situ synthesis process.
PG - 64
LID - 10.1007/s10544-020-00520-5 [doi]
AB - A novel carbon nanotube-collagen@hydroxyapatite (CNT-Col@HA) composite with
good
mechanical and biological properties was fabricated successfully by a multi
in situ
synthesis process, which can be used to repair or replace the damaged bone
tissues.
The carbon nanotube (CNT)/hydroxyapatite (HA) composite powders were firstly
synthesized by the in situ chemical vapor deposition method. After the
acidification
of CNTs, the collagen (Col) molecules were covalently grafted onto the
surface of
CNTs in situ by the formation of amide linkages, obtaining Col-encapsulated
CNTs
powders. And then, a HA layer was deposited in situ onto the Col-encapsulated
CNTs
to form HA- and Col-encapsulated CNTs, consequently the ideal CNT-Col@HA
composite
was fabricated by the powder metallurgy method, and its mechanical and
biological
properties were investigated. The results showed that, the multi in situ
synthesis
process ensured the homogeneous dispersion of CNTs in HA matrix, and via the
intermediate layer of Col, the close chemical bonding between CNT
reinforcements and
HA matrix was obtained, thereby the flexural strength and fracture toughness
of the
in situ synthesized 3 wt.% CNT-Col@HA composite were increased by
approximately
74.2% and 274.6% compared with those of pure HA bulk, and better cell
adhesion,
spreading and proliferation were also observed on the in situ synthesized
CNT-Col@HA
composites. Therefore, the obtained composites in this work have great
potential to
be applied as implant material in clinic.
FAU - Li, Haipeng
AU - Li H
AD - School of Materials Science and Engineering, Hebei University of Technology,
Tianjin, 300130, China.
AD - Research Institute for Energy Equipment Materials, Hebei University of
Technology,
FAU - Sun, Xiwen
AU - Sun X
FAU - Li, Yuanjun
AU - Li Y
FAU - Wang, Hongshui
AU - Wang H
FAU - Li, Baoe
AU - Li B
Tianjin, 300130, China. libaoe@hotmail.com.
FAU - Liang, Chunyong
AU - Liang C
Tianjin, 300130, China. liangcyhebut@163.com.
AD - Research Institute for Energy Equipment Materials, Hebei University of
Technology,
Tianjin, 300130, China. liangcyhebut@163.com.
LA - eng
GR - No. 51201056, No. 51771069, No. 51901239/National Natural Science Foundation
of
China/
GR - No. 16JCYBJC43400/Natural Science Foundation of Tianjin City/
GR - No. E2020202028/Natural Science Foundation of Hebei Province/
DEP - 20200908
PL - United States
TA - Biomed Microdevices
JT - Biomedical microdevices
JID - 100887374
SB - IM
OTO - NOTNLM
OT - *Carbon nanotubes
OT - *Collagen
OT - *Hydroxyapatite
OT - *In situ synthesis
OT - *Mechanical and biological property
EDAT- 2020/09/09 06:00
MHDA- 2020/09/09 06:00
CRDT- 2020/09/08 12:15
PHST- 2020/09/08 12:15 [entrez]
PHST- 2020/09/09 06:00 [pubmed]
PHST- 2020/09/09 06:00 [medline]
AID - 10.1007/s10544-020-00520-5 [pii]
AID - 10.1007/s10544-020-00520-5 [doi]
PST - epublish
SO - Biomed Microdevices. 2020 Sep 8;22(4):64. doi: 10.1007/s10544-020-00520-5.
PMID- 24498185
OWN - NLM
STAT- MEDLINE
DCOM- 20141015
LR - 20181113
IS - 1932-6203 (Linking)
VI - 9
IP - 1
DP - 2014
TI - Characterization of mechanical and biological properties of 3-D scaffolds
reinforced
with zinc oxide for bone tissue engineering.
PG - e87755
LID - e87755
AB - A scaffold for bone tissue engineering should have highly interconnected
porous
structure, appropriate mechanical and biological properties. In this work, we
fabricated well-interconnected porous β-tricalcium phosphate (β-TCP)

scaffolds via
selective laser sintering (SLS). We found that the mechanical and biological
properties of the scaffolds were improved by doping of zinc oxide (ZnO). Our
data
showed that the fracture toughness increased from 1.09 to 1.40 MPam(1/2), and
the
compressive strength increased from 3.01 to 17.89 MPa when the content of ZnO
increased from 0 to 2.5 wt%. It is hypothesized that the increase of ZnO

would lead
to a reduction in grain size and an increase in density of the strut.
However, the
fracture toughness and compressive strength decreased with further increasing
of ZnO
content, which may be due to the sharp increase in grain size. The
biocompatibility
of the scaffolds was investigated by analyzing the adhesion and the
morphology of
human osteoblast-like MG-63 cells cultured on the surfaces of the scaffolds.
The
scaffolds exhibited better and better ability to support cell attachment and
proliferation when the content of ZnO increased from 0 to 2.5 wt%. Moreover,
a bone
like apatite layer formed on the surfaces of the scaffolds after incubation
in
simulated body fluid (SBF), indicating an ability of osteoinduction and
osteoconduction. In summary, interconnected porous β-TCP scaffolds doped with
ZnO
were successfully fabricated and revealed good mechanical and biological
properties,
which may be used for bone repair and replacement potentially.
FAU - Feng, Pei
AU - Feng P
University, Changsha, Hunan Province, P. R. China.

FAU - Wei, Pingpin
AU - Wei P
AD - Cancer Research Institute, Central South University, Changsha, Hunan
Province, P. R.
China.
FAU - Shuai, Cijun
AU - Shuai C
University, Changsha, Hunan Province, P. R. China ; Department of

Regenerative
Medicine & Cell Biology, Medical University of South Carolina, Charleston,
South
Carolina, United States of America.
FAU - Peng, Shuping
AU - Peng S
AD - Cancer Research Institute, Central South University, Changsha, Hunan
Province, P. R.
China ; Department of Obstetrics, Gynecology and Reproductive Sciences, Yale
University School of Medicine, New Haven, Connecticut, United States of
America.
LA - eng
DEP - 20140131
TA - PLoS One
JT - PloS one
JID - 101285081
RN - SOI2LOH54Z (Zinc Oxide)
SB - IM
MH - Cell Line
MH - Humans
MH - *Materials Testing
MH - Osteoblasts/cytology/*metabolism
MH - *Tissue Engineering
MH - Zinc Oxide/*chemistry
PMC - PMC3909231
exist.
EDAT- 2014/02/06 06:00
MHDA- 2014/10/16 06:00
CRDT- 2014/02/06 06:00
PHST- 2013/06/05 00:00 [received]
PHST- 2014/01/02 00:00 [accepted]
PHST- 2014/02/06 06:00 [entrez]
PHST- 2014/02/06 06:00 [pubmed]
PHST- 2014/10/16 06:00 [medline]
PST - epublish
SO - PLoS One. 2014 Jan 31;9(1):e87755. doi: 10.1371/journal.pone.0087755.
eCollection
2014.
PMID- 25822264
OWN - NLM
STAT- MEDLINE
DCOM- 20160706
LR - 20190118
IS - 1617-7959 (Print)
IS - 1617-7940 (Linking)
VI - 14
IP - 6
DP - 2015 Nov
TI - Mechanical microenvironments and protein expression associated with formation
of
different skeletal tissues during bone healing.
PG - 1239-53
LID - 10.1007/s10237-015-0670-4 [doi]
AB - Uncovering the mechanisms of the sensitivity of bone healing to mechanical
factors
is critical for understanding the basic biology and mechanobiology of the
skeleton,
as well as for enhancing clinical treatment of bone injuries. This study
refined an
experimental method of measuring the strain microenvironment at the site of a
bone
injury during bone healing. This method used a rat model in which a well-
controlled
bending motion was applied to an osteotomy to induce the formation of
pseudarthrosis
that is composed of a range of skeletal tissues, including woven bone,
cartilage,
fibrocartilage, fibrous tissue, and clot tissue. The goal of this study was
to
identify both the features of the strain microenvironment associated with
formation
of these different tissues and the expression of proteins frequently
implicated in
sensing and transducing mechanical cues. By pairing the strain measurements
with
histological analyses that identified the regions in which each tissue type
formed,
we found that formation of the different tissue types occurs in distinct
strain
microenvironments and that the type of tissue formed is correlated most
strongly to
the local magnitudes of extensional and shear strains. Weaker correlations
were
found for dilatation. Immunohistochemical analyses of focal adhesion kinase
and rho
family proteins RhoA and CDC42 revealed differences within the cartilaginous
tissues
in the calluses from the pseudarthrosis model as compared to fracture
calluses
undergoing normal endochondral bone repair. These findings suggest the
involvement
of these proteins in the way by which mechanical stimuli modulate the process
of
cartilage formation during bone healing.
FAU - Miller, Gregory J
AU - Miller GJ
AD - Department of Mechanical Engineering, Boston University, Boston, MA, USA.
FAU - Gerstenfeld, Louis C
AU - Gerstenfeld LC
AD - Department of Orthopaedic Surgery, Boston University School of Medicine,
Boston, MA,
USA.
FAU - Morgan, Elise F
AU - Morgan EF
AD - Department of Mechanical Engineering, Boston University, Boston, MA, USA.
efmorgan@bu.edu.
AD - Department of Orthopaedic Surgery, Boston University School of Medicine,
Boston, MA,
USA. efmorgan@bu.edu.
LA - eng
GR - UL1 RR025771/RR/NCRR NIH HHS/United States
GR - AR53353/AR/NIAMS NIH HHS/United States
GR - UL1RR025771/RR/NCRR NIH HHS/United States
DEP - 20150331
TA - Biomech Model Mechanobiol
JT - Biomechanics and modeling in mechanobiology
JID - 101135325
RN - 0 (Extracellular Matrix Proteins)
SB - IM
SB - S
MH - Animals
MH - Cellular Microenvironment/physiology
MH - Computer Simulation
MH - Extracellular Matrix/*physiology
MH - Extracellular Matrix Proteins/*physiology
MH - Femoral Fractures/*physiopathology
MH - Fracture Healing/*physiology
MH - Gene Expression Regulation
MH - Male
MH - *Mechanotransduction, Cellular
MH - *Models, Biological
MH - Rats
PMC - PMC5608650
MID - NIHMS851868
OTO - NOTNLM
OT - FAK
OT - Mechanical environment
OT - Pseudarthrosis
OT - RhoA
OT - Tissue differentiation
EDAT- 2015/03/31 06:00
MHDA- 2016/07/07 06:00
CRDT- 2015/03/31 06:00
PHST- 2014/10/23 00:00 [received]
PHST- 2015/03/23 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2016/07/07 06:00 [medline]
AID - 10.1007/s10237-015-0670-4 [pii]
AID - 10.1007/s10237-015-0670-4 [doi]
PST - ppublish
SO - Biomech Model Mechanobiol. 2015 Nov;14(6):1239-53. doi: 10.1007/s10237-015-
0670-4.
Epub 2015 Mar 31.
PMID- 29702291
OWN - NLM
STAT- MEDLINE
DCOM- 20190522
LR - 20190522
IS - 1742-7061 (Linking)
VI - 74
DP - 2018 Jul 1
TI - Multilayered coating of titanium implants promotes coupled osteogenesis and
angiogenesis in vitro and in vivo.
PG - 489-504
LID - S1742-7061(18)30246-0 [pii]
LID - 10.1016/j.actbio.2018.04.043 [doi]
AB - We used surface-modified titanium (Ti) substrates with a multilayered
structure
composed of chitosan-catechol (Chi-C), gelatin (Gel) and hydroxyapatite (HA)
nanofibers, which were previously shown to improve osteogenesis, as a
platform to
investigate the interaction of osteogenesis and angiogenesis during bone
healing.
Combined techniques of Transwell co-culture, wound healing assay, enzyme
linked
immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction
(qRT-PCR), western blotting and immunohistochemical staining were used to

evaluate
adhesion, morphology and migration of adipose-derived mesenchymal stem cells
(Ad-MSCs) and human umbilical vein endothelial cells (HUVECs) grown on
different Ti
substrates. We investigated the effect of substrates on the osteogenic
differentiation of Ad-MSCs and reciprocal paracrine effects of Ad-MSCs on
HUVECs or
vice versa. The multilayered Ti substrates directly regulated the cellular
functions
of Ad-MSCs and angiogenic HUVECs and mediated communication between them by
enhancing paracrine effects via cell-matrix interactions in vitro. The in
vivo
results showed that the change of microenvironment induced by surface-
modified Ti
implants promoted the adhesion, recruitment and proliferation of MSCs and
facilitated coupled osteogenesis and angiogenesis in bone healing. The study
proved
that multilayer-film-coated Ti substrates positively mediated cellular
biological
function in vitro and improved bone healing in vivo. STATEMENT OF
SIGNIFICANCE:
Recent studies have revealed that osteogenesis and angiogenesis are coupled,
and
that communication between osteoblasts and endothelial cells is essential for
bone
healing and remodeling processes; however, these conclusions only result from
in
vitro studies or in vivo studies using transgenic murine models. Relatively
little
is known about the communication between osteoblasts and endothelial cells in
peri-implants during bone healing processes. Our results revealed the

cellular/molecular mechanism of how multilayered Ti substrates mediate
reciprocal
paracrine effects between adipose-derived mesenchymal stem cells and human
umbilical
vein endothelial cells; moreover, the interactions between the cell-matrix
and
peri-implant was proven in vivo with enhanced bone healing. This study
contributes
to our understanding of the fundamental mechanisms of angiogenesis and
osteogenesis
that affect peri-implantation, and thus, provides new insights into the
design of
future high-quality orthopedic implants.
reserved.
FAU - Chen, Weizhen
AU - Chen W
AD - Key Laboratory of Biorheological Science and Technology, Ministry of
Education
College of Bioengineering, Chongqing University, Chongqing 400044, China;
First
Affiliated Hospital, College of Medicine of Zhejiang University, & Key
Laboratory of
Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou,
Zhejiang
310003, China.
FAU - Xu, Kui
AU - Xu K
AD - Biomedical Engineering Center, Medical School of Ningbo University, Ningbo,
Zhejiang
315211, China.
FAU - Tao, Bailong
AU - Tao B
Education
College of Bioengineering, Chongqing University, Chongqing 400044, China.
FAU - Dai, Liangliang
AU - Dai L
Education
FAU - Yu, Yonglin
AU - Yu Y
Education
FAU - Mu, Caiyun
AU - Mu C
Education
FAU - Shen, Xinkun
AU - Shen X
Education
FAU - Hu, Yan
AU - Hu Y
Education
FAU - He, Ye
AU - He Y
Education
FAU - Cai, Kaiyong
AU - Cai K
Education
College of Bioengineering, Chongqing University, Chongqing 400044, China;
Chongqing
Collaborative Innovation Center for Minimally-Invasive and Noninvasive
Medicine,
Chongqing 400016, China. Electronic address: kaiyong_cai@cqu.edu.cn.
LA - eng
DEP - 20180425
PL - England
TA - Acta Biomater
JID - 101233144
SB - IM
MH - Animals
MH - Coated Materials, Biocompatible/*chemistry
MH - Female
MH - Human Umbilical Vein Endothelial Cells/cytology/*metabolism
MH - Humans
MH - *Implants, Experimental
MH - Mesenchymal Stem Cells/cytology/*metabolism
MH - *Osteogenesis
MH - Rats
OTO - NOTNLM
OT - *Angiogenesis
OT - *Bone healing
OT - *Molecular mechanism
OT - *Osteogenesis
OT - *Titanium substrates
EDAT- 2018/04/28 06:00
MHDA- 2019/05/23 06:00
CRDT- 2018/04/28 06:00
PHST- 2017/12/20 00:00 [received]
PHST- 2018/03/27 00:00 [revised]
PHST- 2018/04/22 00:00 [accepted]
PHST- 2018/04/28 06:00 [pubmed]
PHST- 2019/05/23 06:00 [medline]
PHST- 2018/04/28 06:00 [entrez]
AID - S1742-7061(18)30246-0 [pii]
AID - 10.1016/j.actbio.2018.04.043 [doi]
PST - ppublish
SO - Acta Biomater. 2018 Jul 1;74:489-504. doi: 10.1016/j.actbio.2018.04.043. Epub
2018
Apr 25.
PMID- 25469892
OWN - NLM
STAT- MEDLINE
DCOM- 20150930
LR - 20181202
IS - 1049-2275 (Linking)
VI - 26
IP - 1
DP - 2015 Jan
TI - Intracranial repair of posttraumatic cerebrospinal fluid rhinorrhea
associated with
recurrent meningitis.
PG - 170-3
LID - 10.1097/SCS.0000000000001181 [doi]
AB - OBJECTIVE: The purposes of this study are to assess the efficacy of our
intracranial
surgery and evaluate the association between failure after first surgical
repair and
the risk factors that have been applied on a group of 13 patients affected by
posttraumatic cerebrospinal fluid rhinorrhea associated with recurrent

meningitis.
METHODS: We retrospectively collected data on 13 patients referred to our
institution. All patients had history of head trauma and experienced 2 or
more
episodes of meningitis. RESULTS: Three of the 13 patients had craniectomy
defect due
to previous trauma and surgery, 9 patients had linear fracture, and 1 patient
had no
apparent fracture line on preoperative radiologic evaluation. Ten of the 13
patients
had identified frontal bone fracture involving the frontal sinus during
surgery.
Dural tear was identified intradurally and was repaired using a fascia lata
graft
with or without fibrin glue. Fibrin glue was applied over the suture in 7
patients.
Three of the 13 patients had large dural defects. CONCLUSIONS: The size of
bone and
dural defect seems to be an important prognostic factor of episodes of
meningitis.
The use of fibrin glue to fixate fascia lata graft did not benefit the
outcome.
FAU - Yaldiz, Can
AU - Yaldiz C
AD - From the *Department of Neurosurgery, Sakarya University Training and
Research
Hospital, Sakarya; †Department of Neurosurgery, İzmir Tepecik Training and
Research
Hospital, İzmir; and ‡Department of Neurosurgery, İzmir Atatürk Training and
Research Hospital, İzmir, Turkey.
FAU - Ozdemir, Nail
AU - Ozdemir N
FAU - Yaman, Onur
AU - Yaman O
FAU - Seyin, İsmail Ertan
AU - Seyin İE
FAU - Oguzoglu, Serdar
AU - Oguzoglu S
LA - eng
PL - United States
JID - 9010410
SB - D
MH - Adolescent
MH - Adult
MH - Aged
MH - Cerebrospinal Fluid Rhinorrhea/etiology/*surgery
MH - Child
MH - Fascia Lata/transplantation
MH - Female
MH - Humans
MH - Male
MH - Meningitis/*complications
MH - Middle Aged
MH - Recurrence
MH - Risk Factors
MH - Skull Fractures/complications/*surgery
MH - Young Adult
EDAT- 2014/12/04 06:00
MHDA- 2015/10/01 06:00
CRDT- 2014/12/04 06:00
PHST- 2014/12/04 06:00 [entrez]
PHST- 2014/12/04 06:00 [pubmed]
PHST- 2015/10/01 06:00 [medline]
AID - 10.1097/SCS.0000000000001181 [doi]
PST - ppublish
SO - J Craniofac Surg. 2015 Jan;26(1):170-3. doi: 10.1097/SCS.0000000000001181.
PMID- 20886648
OWN - NLM
STAT- MEDLINE
DCOM- 20110127
LR - 20171116
IS - 0736-0266 (Linking)
VI - 29
IP - 2
DP - 2011 Feb
TI - The effects of low-intensity pulsed ultrasound upon diabetic fracture
healing.
PG - 181-8
LID - 10.1002/jor.21223 [doi]
AB - In the United States, over 17 million people are diagnosed with type 1
diabetes
mellitus (DM) with its inherent morbidity of delayed bone healing and
nonunion.
Recent studies demonstrate the utility of pulsed low-intensity ultrasound
(LIPUS) to
facilitate fracture healing. The current study evaluated the effects of daily
application of LIPUS on mid-diaphyseal femoral fracture growth factor

expression,
cartilage formation, and neovascularization in DM and non-DM BB Wistar rats.
Polymerase chain reaction (PCR) and ELISA assays were used to measure and
quantify
growth factor expression. Histomorphometry assessed cartilage formation while
immunohistochemical staining for PECAM evaluated neovascularization at the

fracture
site. In accordance with previous studies, LIPUS was shown to increase growth
factor
expression and cartilage formation. Our study also demonstrated an increase
in
fracture callus neovascularization with the addition of LIPUS. The DM group
showed
impaired growth factor expression, cartilage formation, and
neovascularization.
However, the addition of LIPUS significantly increased all parameters so that
the DM
group resembled that of the non-DM group. These findings suggest a potential
role of
LIPUS as an adjunct for DM fracture treatment.
FAU - Coords, Michael
AU - Coords M
AD - Department of Orthopaedics, University of Medicine and Dentistry of New
Jersey-New
Jersey Medical School, 185 South Orange Avenue, Newark, New Jersey 07103,
USA.
FAU - Breitbart, Eric
AU - Breitbart E
FAU - Paglia, David
AU - Paglia D
FAU - Kappy, Nikolas
AU - Kappy N
FAU - Gandhi, Ankur
AU - Gandhi A
FAU - Cottrell, Jessica
AU - Cottrell J
FAU - Cedeno, Natalie
AU - Cedeno N
FAU - Pounder, Neill
AU - Pounder N
AU - O'Connor JP
AU - Lin SS
LA - eng
DEP - 20100930
PL - United States
TA - J Orthop Res
Research
Society
JID - 8404726
SB - IM
MH - Animals
MH - Chondrogenesis
MH - Diabetes Complications/*therapy
MH - Femoral Fractures/complications/metabolism/pathology/*therapy
MH - Femur/blood supply/metabolism/pathology
MH - Intercellular Signaling Peptides and Proteins/*metabolism
MH - Rats
MH - Rats, Wistar
MH - *Ultrasonic Therapy
EDAT- 2010/10/05 06:00
MHDA- 2011/01/29 06:00
CRDT- 2010/10/02 06:00
PHST- 2010/01/20 00:00 [received]
PHST- 2010/06/18 00:00 [accepted]
PHST- 2010/10/02 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2011/01/29 06:00 [medline]
AID - 10.1002/jor.21223 [doi]
PST - ppublish
SO - J Orthop Res. 2011 Feb;29(2):181-8. doi: 10.1002/jor.21223. Epub 2010 Sep 30.
PMID- 29953336
OWN - NLM
STAT- MEDLINE
DCOM- 20190624
LR - 20190624
IS - 0361-7734 (Linking)
VI - 44
IP - 1
DP - 2019 Jan/Feb
TI - Color Repair of a Composite Resin Restoration.
PG - 1-7
LID - 10.2341/17-079-T [doi]
AB - Fractured teeth with both enamel and dentin involvement might be treated with
adhesive composite resin restorations. In cases where a perfect color match

between
the composite restoration and the remaining tooth structure is not achieved,
a
repair might be carried out to correct the color of restoration. This
procedure
avoids the restoration replacement, preserving tooth structure without
compromising
the esthetic outcome.
FAU - Rauber, G B
AU - Rauber GB
FAU - Taguchi, Cmc
AU - Taguchi C
FAU - Padilha, Acl
AU - Padilha A
FAU - de Re Silveira, R C
AU - de Re Silveira RC
FAU - Bernardon, J K
AU - Bernardon JK
FAU - Baratieri, L N
AU - Baratieri LN
LA - eng
PT - Case Reports
DEP - 20180628
PL - United States
TA - Oper Dent
JT - Operative dentistry
JID - 7605679
SB - D
MH - Child
MH - Color
MH - Composite Resins/*chemistry
MH - Esthetics, Dental
MH - Humans
MH - Incisor/*injuries
MH - Male
MH - Maxilla
MH - Soccer/*injuries
EDAT- 2018/06/29 06:00
MHDA- 2019/06/25 06:00
CRDT- 2018/06/29 06:00
PHST- 2018/06/29 06:00 [pubmed]
PHST- 2019/06/25 06:00 [medline]
PHST- 2018/06/29 06:00 [entrez]
AID - 10.2341/17-079-T [doi]
PST - ppublish
SO - Oper Dent. 2019 Jan/Feb;44(1):1-7. doi: 10.2341/17-079-T. Epub 2018 Jun 28.
PMID- 24781604
OWN - NLM
STAT- MEDLINE
DCOM- 20150106
LR - 20170214
IS - 1022-5536 (Linking)
VI - 22
IP - 1
DP - 2014 Apr
TI - Outcome of locking compression plating for proximal humeral fractures: a
prospective
study.
PG - 4-8
AB - PURPOSE: To evaluate the outcome of open reduction and internal fixation
using
locking compression plates for proximal humeral fractures. METHODS: 54 men
and 16
women aged 28 to 79 (mean, 54) years underwent open reduction and internal
fixation
using a locking compression plate for closed 2-part (n=22), 3-part (n=38),
and
4-part (n=10) proximal humeral fractures. 10 of the patients also had
dislocation of
the humeral head; 4 had fractures extending to the shaft. Wound condition,
functional outcome, bone union, amount of collapse, and malalignment were
assessed.
Functional outcome was assessed using the Constant-Murley score. RESULTS; The
mean
follow-up period was 15 (range, 6-24) months. All fractures achieved union
after a
mean of 9 (range, 6-12) weeks. The mean Constant-Murley scores for the
injured and
normal shoulders were 72 and 82, respectively (88% of normal). The final
outcome was
excellent in 14 patients, good in 28, moderate in 22, and poor in 6. In the
latter 6
patients, 2 had screw penetration, 2 had plate impingement, one had a mal-
reduced
greater tuberosity, and one had adhesive capsulitis. All were preventable. In
all,
18 patients had 20 complications: subacromial impingement of the plate (n=6),
mal-reduction of the greater tuberosity (n=6), screw penetration (n=2),

adhesive
capsulitis (n=2), superficial infection (n=2), and haematoma (n=2); 12 of
these
complications were technique-related. CONCLUSION: Locking proximal humeral
plates
enabled stable fixation in all Neer-type proximal humeral fractures. Most
complications were technique-related.
FAU - Chowdary, Umapathi
AU - Chowdary U
AD - Department of Orthopaedics, Srinivas Institute of Medical Sciences & Research
Center, Mukka, Mangalore, Karnataka, India.

FAU - Prasad, Hari
AU - Prasad H
AD - Department of Orthopaedics, Sri Devaraj Urs Medical College, Tamaka, Kolar,
Karnataka, India.
FAU - Subramanyam, P Krishna
AU - Subramanyam PK
AD - Department of Orthopaedics, Kamineni Hospitals, LB Nagar, Hyderabad, Andhra
Pradesh,
India.
LA - eng
PL - England
TA - J Orthop Surg (Hong Kong)
JT - Journal of orthopaedic surgery (Hong Kong)
JID - 9440382
SB - IM
CIN - J Orthop Surg (Hong Kong). 2014 Aug;22(2):269-70. PMID: 25163971
MH - Adult
MH - Aged
MH - *Bone Plates
MH - Female
MH - Fracture Fixation, Internal/*instrumentation
MH - Fractures, Closed/diagnostic imaging/*surgery
MH - Humans
MH - Male
MH - Middle Aged
MH - Radiography
MH - Shoulder Fractures/diagnostic imaging/*surgery
OTO - NOTNLM
OT - bone plates
OT - fracture fixation, internal
OT - humeral fractures
OT - shoulder fractures
EDAT- 2014/05/02 06:00
MHDA- 2015/01/07 06:00
CRDT- 2014/05/01 06:00
PHST- 2014/05/01 06:00 [entrez]
PHST- 2014/05/02 06:00 [pubmed]
PHST- 2015/01/07 06:00 [medline]
AID - 10.1177/230949901402200104 [doi]
PST - ppublish
SO - J Orthop Surg (Hong Kong). 2014 Apr;22(1):4-8. doi:
10.1177/230949901402200104.
PMID- 24204147
OWN - NLM
STAT- MEDLINE
DCOM- 20140609
LR - 20181202
IS - 1176-9114 (Print)
IS - 1176-9114 (Linking)
VI - 8
DP - 2013
TI - Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-
caprolactone
scaffolds for bone tissue engineering applications.
PG - 4197-213
LID - 10.2147/IJN.S50685 [doi]
AB - The regeneration of functional tissue in osseous defects is a formidable
challenge
in orthopedic surgery. In the present study, a novel biomimetic composite
scaffold,
here called nano-hydroxyapatite (HA)/poly-ε-caprolactone (PCL) was fabricated
using
a selective laser sintering technique. The macrostructure, morphology, and
mechanical strength of the scaffolds were characterized. Scanning electronic
microscopy (SEM) showed that the nano-HA/PCL scaffolds exhibited predesigned,
well-ordered macropores and interconnected micropores. The scaffolds have a

range of
porosity from 78.54% to 70.31%, and a corresponding compressive strength of
1.38 MPa
to 3.17 MPa. Human bone marrow stromal cells were seeded onto the nano-HA/PCL
or PCL
scaffolds and cultured for 28 days in vitro. As indicated by the level of
cell
attachment and proliferation, the nano-HA/PCL showed excellent
biocompatibility,
comparable to that of PCL scaffolds. The hydrophilicity, mineralization,
alkaline
phosphatase activity, and Alizarin Red S staining indicated that the nano-
HA/PCL
scaffolds are more bioactive than the PCL scaffolds in vitro. Measurements of
recombinant human bone morphogenetic protein-2 (rhBMP-2) release kinetics
showed
that after nano-HA was added, the material increased the rate of rhBMP-2
release. To
investigate the in vivo biocompatibility and osteogenesis of the composite
scaffolds, both nano-HA/PCL scaffolds and PCL scaffolds were implanted in
rabbit
femur defects for 3, 6, and 9 weeks. The wounds were studied radiographically
and
histologically. The in vivo results showed that both nano-HA/PCL composite
scaffolds
and PCL scaffolds exhibited good biocompatibility. However, the nano-HA/PCL
scaffolds enhanced the efficiency of new bone formation more than PCL
scaffolds and
fulfilled all the basic requirements of bone tissue engineering scaffolds.
Thus,
they show large potential for use in orthopedic and reconstructive surgery.
FAU - Xia, Yan
AU - Xia Y
AD - Department of Orthopedics, Changhai Hospital, Second Military Medical
University,
Shanghai, People's Republic of China.
FAU - Zhou, Panyu
AU - Zhou P
FAU - Cheng, Xiaosong
AU - Cheng X
FAU - Xie, Yang
AU - Xie Y
FAU - Liang, Chong
AU - Liang C
FAU - Li, Chao
AU - Li C
FAU - Xu, Shuogui
AU - Xu S
LA - eng
DEP - 20131101
TA - Int J Nanomedicine
JT - International journal of nanomedicine
JID - 101263847
RN - 0 (Polyesters)
RN - 24980-41-4 (polycaprolactone)
SB - IM
MH - Animals
MH - Biocompatible Materials/chemical synthesis/chemistry/pharmacology
MH - Bone Morphogenetic Protein 2/chemistry/pharmacokinetics/pharmacology
MH - Durapatite/*chemistry/pharmacology
MH - Humans
MH - Hydrophobic and Hydrophilic Interactions
MH - Nanostructures/*chemistry
MH - Polyesters/*chemistry/pharmacology
MH - Rabbits
MH - Tissue Engineering/*instrumentation/methods
PMC - PMC3818022
OTO - NOTNLM
OT - biomimetic composite scaffold
OT - orthopedic surgery
OT - osseous defects
OT - reconstructive surgery
EDAT- 2013/11/10 06:00
MHDA- 2014/06/10 06:00
CRDT- 2013/11/09 06:00
PHST- 2013/11/09 06:00 [entrez]
PHST- 2013/11/10 06:00 [pubmed]
PHST- 2014/06/10 06:00 [medline]
AID - ijn-8-4197 [pii]
AID - 10.2147/IJN.S50685 [doi]
PST - ppublish
SO - Int J Nanomedicine. 2013;8:4197-213. doi: 10.2147/IJN.S50685. Epub 2013 Nov
1.
PMID- 27329730
OWN - NLM
STAT- MEDLINE
DCOM- 20180507
LR - 20181202
IS - 2045-2322 (Linking)
VI - 6
DP - 2016 Jun 22
TI - Effects of local delivery of BMP2, zoledronate and their combination on bone
microarchitecture, biomechanics and bone turnover in osteoporotic rabbits.
PG - 28537
LID - 10.1038/srep28537 [doi]
LID - 28537
AB - The hip fracture is one major clinical challenge associated with
osteoporosis,
resulting in heavy socioeconomic burdens and high mortality. Systemic
therapies of
anti-osteoporosis drugs are expensive, time-consuming and also evoke
substantial
side effects, which fails to provide early protection from fractures.
Accumulating
evidence demonstrates the high bioavailability and therapeutic efficacy of
local
drug delivery in accelerating facture healing and bone defect repair. This
study
aims at investigating the effects of local delivery of BMP2 and zoledronate
(two
promising anabolic/anti-catobolic reagents) encapsulated by fibrin sealants
into
femoral necks on regulating bone quality and remodeling in osteoporotic
rabbits
subjected to combined ovariectomy and glucocorticoid injection. We show that
6-week
BMP2 delivery exhibited more prominent effect on mitigating trabecular bone
microarchitecture deterioration and mechanical strength reduction of femoral
necks
than local zoledronate treatment. BMP2 plus zoledronate showed more
significant
improvement of bone microstructure, mechanical strength and bone formation
rate at
12 weeks post injection than single BMP2 or zoledronate delivery via μCT,
biomechanical, histomorphometric and serum biochemical analyses. This study
enriches
our knowledge for understanding the availability of local drug delivery for
improving bone quantity and quality, which may lead to earlier, safer and
more
efficient protection from osteoporosis-induced fractures in clinics.
FAU - Jing, Da
AU - Jing D
AD - Department of Biomedical Engineering, Fourth Military Medical University,
Xi'an,
China.
AD - Institute of Orthopaedics, Xijing hospital, Fourth Military Medical
University,
Xi'an, China.
FAU - Hao, Xuguang
AU - Hao X
AD - Department of orthopaedics, the Fifth Hospital of Harbin, Harbin, China.
FAU - Xu, Fang
AU - Xu F
AD - Department of Pharmacy, Zhejiang University of Technology, Hangzhou,
Zhejiang,
China.
FAU - Liu, Jian
AU - Liu J
University,
Xi'an, China.
FAU - Xu, Fei
AU - Xu F
AD - Department of Radiation Oncology, PLA 302 hospital, Beijing, China.
FAU - Luo, Erping
AU - Luo E
AD - Department of Biomedical Engineering, Fourth Military Medical University,
Xi'an,
China.
FAU - Meng, Guolin
AU - Meng G
University,
Xi'an, China.
LA - eng
DEP - 20160622
TA - Sci Rep
JID - 101563288
RN - 0 (Diphosphonates)
RN - 0 (Imidazoles)
RN - 6XC1PAD3KF (Zoledronic Acid)
SB - IM
MH - Animals
MH - Bone Density Conservation Agents/*administration & dosage
MH - Bone Morphogenetic Protein 2/*administration & dosage
MH - Bone Remodeling/drug effects
MH - Diphosphonates/*administration & dosage
MH - Drug Delivery Systems
MH - Female
MH - Femur Neck/diagnostic imaging/drug effects/physiopathology
MH - Fibrin Tissue Adhesive/administration & dosage
MH - Imidazoles/*administration & dosage
MH - Osteoporosis/diagnostic imaging/*drug therapy/physiopathology
MH - Ovariectomy
MH - Rabbits
MH - Recombinant Proteins/administration & dosage
MH - Zoledronic Acid
PMC - PMC4916507
EDAT- 2016/06/23 06:00
MHDA- 2018/05/08 06:00
CRDT- 2016/06/23 06:00
PHST- 2015/11/24 00:00 [received]
PHST- 2016/06/03 00:00 [accepted]
PHST- 2016/06/23 06:00 [entrez]
PHST- 2016/06/23 06:00 [pubmed]
PHST- 2018/05/08 06:00 [medline]
AID - srep28537 [pii]
AID - 10.1038/srep28537 [doi]
PST - epublish
SO - Sci Rep. 2016 Jun 22;6:28537. doi: 10.1038/srep28537.
PMID- 24485794
OWN - NLM
STAT- MEDLINE
DCOM- 20141015
LR - 20171116
IS - 0142-9612 (Linking)
VI - 35
IP - 12
DP - 2014 Apr
TI - The role of fucosylation in the promotion of endothelial progenitor cells in
neovascularization and bone repair.
PG - 3777-85
LID - S0142-9612(14)00027-1 [pii]
AB - Bone marrow-derived endothelial progenitor cells (EPCs) are being tested as a
therapy to treat a variety of ischemic diseases. Poor homing to targeted

tissues is
one of the major factors limiting the therapeutic efficacy of EPCs. Here, we
show
that human cord blood-derived EPCs expressed little sialyl Lewis X (sLe(x))
antigen
that is necessary for selectin-mediated cell-cell interactions. Expression of
α1,3-fucosyltransferase VI (FucT VI) in the EPCs enhanced sLe(x) synthesis,

E- and
P-selectin-binding, and EPC adhesion to tumor necrosis factor-α-stimulated
human
umbilical vein endothelial cells in culture. In a mouse model of hind limb
ischemia,
in which EPCs were injected intravenously, FucT VI expression increased EPC
homing,
neovascularization, and blood flow in ischemic muscles. In another mouse
model of
femoral fracture, FucT VI-expressing EPCs were more efficient than control
EPCs in
targeting to peri-fracture tissues to enhance angiogenesis, blood flow and
bone
repair. These results indicate that fucosylated EPCs may be used to as an
improved
cellular source to treat ischemic diseases.
FAU - Sun, Shengxuan
AU - Sun S
AD - The Cyrus Tang Hematology Center, Soochow University, Suzhou 215123, China;
The
Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
FAU - Liu, Zhenzhen
AU - Liu Z
AD - The Cyrus Tang Hematology Center, Soochow University, Suzhou 215123, China.
FAU - Zhou, Haibin
AU - Zhou H
AD - The Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
FAU - Li, Guoqiang
AU - Li G
AD - The Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
FAU - Liu, Meng
AU - Liu M
FAU - Yao, Jiannan
AU - Yao J
AD - Department of Oncology, Chao-Yang Hospital, Capital Medical University,
Beijing
100020, China.
FAU - Zhou, Tiantian
AU - Zhou T
FAU - An, Guangyu
AU - An G
AD - Department of Oncology, Chao-Yang Hospital, Capital Medical University,
Beijing
100020, China. Electronic address: anguangyu@hotmail.com.
FAU - Wu, Qingyu
AU - Wu Q
Molecular Cardiology, Cleveland Clinic, Cleveland, OH 44195, USA. Electronic
address: wuq@ccf.org.
FAU - Dong, Ningzheng
AU - Dong N
The MOH
Key Laboratory of Thrombosis and Hemostasis, Jiangsu Institute of Hematology,
the
First Affiliated Hospital of Soochow University, Suzhou 215006, China.
Electronic
address: ningzhengdong@suda.edu.cn.
LA - eng
DEP - 20140130
PL - Netherlands
TA - Biomaterials
JT - Biomaterials
JID - 8100316
RN - 28RYY2IV3F (Fucose)
SB - IM
MH - Animals
MH - *Bone Development
MH - Cell Adhesion
MH - Endothelial Cells/cytology
MH - Fucose/*metabolism
MH - Hindlimb/blood supply
MH - Humans
MH - Ischemia/therapy
MH - Mice
MH - Stem Cells/*cytology
OTO - NOTNLM
OT - Angiogenesis
OT - Bone repair
OT - Endothelial progenitor cells
OT - Fucosylation
OT - Ischemic disease
OT - Selectins
EDAT- 2014/02/04 06:00
MHDA- 2014/10/16 06:00
CRDT- 2014/02/04 06:00
PHST- 2013/11/20 00:00 [received]
PHST- 2014/01/09 00:00 [accepted]
PHST- 2014/02/04 06:00 [entrez]
PHST- 2014/02/04 06:00 [pubmed]
PHST- 2014/10/16 06:00 [medline]
AID - S0142-9612(14)00027-1 [pii]
PST - ppublish
SO - Biomaterials. 2014 Apr;35(12):3777-85. doi:
10.1016/j.biomaterials.2014.01.025. Epub
2014 Jan 30.
PMID- 28580902
OWN - NLM
STAT- MEDLINE
DCOM- 20180320
LR - 20180320
IS - 1748-6041 (Linking)
VI - 12
IP - 3
DP - 2017 Jun 5
TI - Enhanced bone formation by strontium modified calcium sulfate hemihydrate in
ovariectomized rat critical-size calvarial defects.
PG - 035004
LID - 10.1088/1748-605X/aa68bc [doi]
AB - The development of a new generation of biomaterials with high osteogenic
ability for
treatment of osteoporotic fractures is being intensively investigated. The
objective
of this paper was to investigate new bone formation in an ovariectomized rat
(OVX
rat) calvarial model of critical size bone defects filled with Sr-containing
α-calcium sulfate hemihydrate (SrCSH) cement compared to an α-calcium sulfate
hemihydrate (α-CSH) cement and empty defect. X-ray diffraction analysis

verified the
partial substitution of Sr(2+) for Ca(2+) did not change the phase
composition of
α-CSH. Scanning electron microscopy showed that Sr-substituted α-CSH
significantly
increased the surface roughness. The effects of Sr substitution on the
biological
properties of SrCSH cement were evaluated by adhesion, proliferation,
alkaline
phosphatase (ALP) activity of osteoblast-like cells MC3T3-E1. The results
showed
that SrCSHs enhanced MC3T3-E1 cell proliferation, differentiation, and ALP
activity.
Furthermore, SrCSH cement was used to repair critical-sized OVX rat calvarial
defects. The in vivo results revealed that SrCSH had good osteogenic
capability and
stimulated new blood vessel formation in a critical sized OVX calvarial
defect
within 12 weeks, suggesting that SrCSH cement has more potential for
application in
bone tissue regeneration.
FAU - Yang, Shenyu
AU - Yang S
AD - Department of Materials Science and Engineering, Jinan University, Guangzhou
510632,
People's Republic of China. Engineering Research Center of Artificial Organs
and
Materials, Ministry of Education, Jinan University, Guangzhou 510632,
People's
Republic of China.
FAU - Wang, Lei
AU - Wang L
FAU - Feng, Shanbao
AU - Feng S
FAU - Yang, Qinmeng
AU - Yang Q
FAU - Yu, Bin
AU - Yu B
FAU - Tu, Mei
AU - Tu M
LA - eng
DEP - 20170605
PL - England
TA - Biomed Mater
JID - 101285195
RN - YZS2RPE8LE (Strontium)
SB - IM
MH - 3T3 Cells
MH - Animals
MH - Bone Substitutes/*chemical synthesis/*therapeutic use
MH - Calcium Sulfate/*chemistry
MH - Female
MH - Mice
MH - *Osteogenesis
MH - Osteoporotic Fractures/pathology/physiopathology/*therapy
MH - Ovariectomy
MH - Rats
MH - Skull Fractures/pathology/physiopathology/*therapy
MH - Strontium/*chemistry
EDAT- 2017/06/06 06:00
MHDA- 2018/03/21 06:00
CRDT- 2017/06/06 06:00
PHST- 2017/06/06 06:00 [entrez]
PHST- 2017/06/06 06:00 [pubmed]
PHST- 2018/03/21 06:00 [medline]
AID - 10.1088/1748-605X/aa68bc [doi]
PST - epublish
SO - Biomed Mater. 2017 Jun 5;12(3):035004. doi: 10.1088/1748-605X/aa68bc.
PMID- 33005533
OWN - NLM
LR - 20201003
IS - 2168-8184 (Print)
IS - 2168-8184 (Linking)
VI - 12
IP - 8
DP - 2020 Aug 29
TI - Negative Pressure Wound Therapy With Flap Reconstruction for Extensive Soft
Tissue
Loss in the Foot: A Case Report.
PG - e10116
LID - 10.7759/cureus.10116 [doi]
LID - e10116
AB - Negative pressure wound therapy (NPWT) can create the healing granulation
tissue
that will form a wound bed for the skin graft, thereby reducing the volume of
the
soft tissue defect. The application of uniform negative pressure, which is
delivered
by vacuum-assisted closure (VAC) therapy, induces a physical response
(macrostrain)
and a biological response (microstrain). The patient in the current case
report
presented with an alleged history of a road traffic accident, sustaining a
crush
injury to his right heel pad, resulting in an open comminuted fracture of the
right
calcaneum with bone loss. A total of seven days of NPWT was allowed. Negative
pressure sponge dressing was then applied in this region and adhesive drapes
were
sealed. Once sealed, suction was set at the continuous pressure of -125 mm
Hg. The
authors noted that the benefits significantly outweigh the costs of the
VAC system,
making it an essential treatment option for patients similar to the one
presented in
this case report.
CI - Copyright © 2020, Vellingiri et al.
FAU - Vellingiri, Kishore
AU - Vellingiri K
AD - Orthopaedics, Sri Devaraj Urs Academy of Higher Education and Research,
Kolar, IND.
FAU - S, Nagakumar J
AU - S NJ
AD - Orthopaedics, Sri Devaraj Urs Academy of Higher Education and Research,
Kolar, IND.
FAU - Hongaiah, Deepak
AU - Hongaiah D
AD - Plastic Surgery, Sri Devaraj Urs Academy of Higher Education and Research,
Kolar,
IND.
LA - eng
PT - Case Reports
DEP - 20200829
TA - Cureus
JT - Cureus
JID - 101596737
PMC - PMC7523747
OTO - NOTNLM
OT - negative pressure wound therapy
COIS- The authors have declared that no competing interests exist.
EDAT- 2020/10/03 06:00
MHDA- 2020/10/03 06:01
CRDT- 2020/10/02 05:45
PHST- 2020/10/02 05:45 [entrez]
PHST- 2020/10/03 06:00 [pubmed]
PHST- 2020/10/03 06:01 [medline]
AID - 10.7759/cureus.10116 [doi]
PST - epublish
SO - Cureus. 2020 Aug 29;12(8):e10116. doi: 10.7759/cureus.10116.
PMID- 31101448
OWN - NLM
STAT- MEDLINE
DCOM- 20200401
LR - 20200401
IS - 0301-5629 (Linking)
VI - 45
IP - 8
DP - 2019 Aug
TI - Effect of Low-Intensity Pulsed Ultrasound Stimulation, Extracorporeal
Shockwaves and
Radial Pressure Waves on Akt, BMP-2, ERK-2, FAK and TGF-β1 During Bone
Healing in
Rat Tibial Defects.
PG - 2140-2161
LID - S0301-5629(19)30150-4 [pii]
LID - 10.1016/j.ultrasmedbio.2019.04.011 [doi]
AB - An experimental study was conducted to determine whether low-intensity pulsed
ultrasound stimulation (LIPUS), extracorporeal shockwave treatment (ESWT) and

radial
pressure wave treatment (RPWT) modulate Akt, bone morphogenetic protein-2
(BMP-2),
extracellular signal-regulated kinase-2 (ERK-2), focal adhesion kinase (FAK)
and
transforming growth factor-β1 (TGF-β1) during bone healing in rat tibial
defects.
Rat tibial defects were exposed to 500 shots of ESWT delivered at 0.12
mJ/mm(2), 500
impulses of RPWT operated at 2.0 bar or to daily 20-min 30 mW/cm(2) LIPUS.
Following
1, 3 and 6 wk, bones were harvested to determine the expression and activity
of Akt,
BMP-2, ERK-2, FAK and TGF-β1. Animals exposed to ultrasound were followed up
to 3
wk. Protein expression and activity were unchanged following LIPUS treatment.
ESWT
increased Akt activity 2.11-fold (p = 0.043) and TGF-β1 expression 9.11-fold
(p = 0.016) at 1 wk and increased FAK activity 2.16-fold (p = 0.047) at 3 wk.
RPWT
increased FAK activity 2.6-fold (p = 0.028) at 3 wk and decreased Akt
expression
0.52-fold (p = 0.05) at 6 wk. In conclusion, the protocols employed for ESWT
and
RPWT modulated distinct signaling pathways during fracture healing, while
LIPUS
standard protocol did not change the usual signaling pathways of the proteins
investigated. Future studies are required to monitor osteogenesis so that the
biologic meaning of our results can be clarified.

CI - Copyright © 2019 World Federation for Ultrasound in Medicine & Biology.
Published by
Elsevier Inc. All rights reserved.
FAU - Buarque de Gusmão, Carlos Vinícius
AU - Buarque de Gusmão CV
AD - Department of Orthopedics and Traumatology, Faculty of Medical Sciences,
State
University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. Electronic
address:
vigusmao@yahoo.com.
FAU - Batista, Nilza Alzira
AU - Batista NA
State
University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
FAU - Vidotto Lemes, Valeria Trombini
AU - Vidotto Lemes VT
State
FAU - Maia Neto, Wilson Leite
AU - Maia Neto WL
State
FAU - de Faria, Lidia Dornelas
AU - de Faria LD
State
FAU - Alves, José Marcos
AU - Alves JM
AD - Electrical Engineering Department, College of Engineering of São Carlos,
University
of São Paulo (USP), São Carlos, São Paulo, Brazil.
FAU - Belangero, William Dias
AU - Belangero WD
State
LA - eng
DEP - 20190514
PL - England
TA - Ultrasound Med Biol
JT - Ultrasound in medicine & biology
JID - 0410553
RN - 0 (Transforming Growth Factor beta2)
RN - EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
SB - IM
MH - Animals
MH - Extracorporeal Shockwave Therapy/methods
MH - Focal Adhesion Protein-Tyrosine Kinases/*metabolism
MH - High-Energy Shock Waves
MH - Immunoblotting
MH - Male
MH - Mitogen-Activated Protein Kinase 1/*metabolism
MH - Proto-Oncogene Proteins c-akt/*metabolism
MH - Rats
MH - Tibia/injuries/*metabolism
MH - Transforming Growth Factor beta2/*metabolism
MH - Ultrasonic Therapy/*methods
MH - Ultrasonic Waves
OTO - NOTNLM
OT - *Bone
OT - *Bone morphogenetic protein
OT - *ERK
OT - *ESWT
OT - *Focal adhesion kinase
OT - *Fracture
OT - *LIPUS
OT - *Osteogenesis
OT - *Radial pressure waves
OT - *Transforming growth factor
EDAT- 2019/05/19 06:00
MHDA- 2020/04/02 06:00
CRDT- 2019/05/19 06:00
PHST- 2018/09/02 00:00 [received]
PHST- 2019/03/24 00:00 [revised]
PHST- 2019/04/07 00:00 [accepted]
PHST- 2019/05/19 06:00 [pubmed]
PHST- 2020/04/02 06:00 [medline]
PHST- 2019/05/19 06:00 [entrez]
AID - S0301-5629(19)30150-4 [pii]
AID - 10.1016/j.ultrasmedbio.2019.04.011 [doi]
PST - ppublish
SO - Ultrasound Med Biol. 2019 Aug;45(8):2140-2161. doi:
10.1016/j.ultrasmedbio.2019.04.011. Epub 2019 May 14.
PMID- 26457873
OWN - NLM
STAT- MEDLINE
DCOM- 20160905
LR - 20181202
IS - 1944-8244 (Print)
IS - 1944-8244 (Linking)
VI - 7
IP - 41
DP - 2015 Oct 21
TI - Microgrooved Polymer Substrates Promote Collective Cell Migration To
Accelerate
Fracture Healing in an in Vitro Model.
PG - 23336-45
LID - 10.1021/acsami.5b07976 [doi]
AB - Surface topography can affect cell adhesion, morphology, polarity,
cytoskeleton
organization, and osteogenesis. However, little is known about the effect of
topography on the fracture healing in repairing nonunion and large bone
defects.
Microgrooved topography on the surface of bone implants may promote cell
migration
into the fracture gap to accelerate fracture healing. To prove this
hypothesis, we
used an in vitro fracture (wound) healing assay on the microgrooved
polycaprolactone
substrates to study the effect of microgroove widths and depths on the
osteoblast-like cell (MG-63) migration and the subsequent healing. We found
that the
microgrooved substrates promoted MG-63 cells to migrate collectively into the
wound
gap, which serves as a fracture model, along the grooves and ridges as
compared with
the flat substrates. Moreover, the groove widths did not show obvious
influence on
the wound healing whereas the smaller groove depths tended to favor the
collective
cell migration and thus subsequent healing. The microgrooved substrates
accelerated
the wound healing by facilitating the collective cell migration into the
wound gaps
but not by promoting the cell proliferation. Furthermore, microgrooves were
also
found to promote the migration of human mesenchymal stem cells (hMSCs) to
heal the
fracture model. Though osteogenic differentiation of hMSCs was not improved
on the
microgrooved substrate, collagen I and minerals deposited by hMSCs were
organized in
a way similar to those in the extracellular matrix of natural bone. These
findings
suggest the necessity in using microgrooved implants in enhancing fracture
healing
in bone repair.
FAU - Zhang, Qing
AU - Zhang Q
AD - Department of Biomedical Engineering, South China University of Technology ,
Guangzhou, Guangdong 510641, China.
AD - School of Materials Science and Engineering, South China University of
Technology ,
FAU - Dong, Hua
AU - Dong H
Technology ,
FAU - Li, Yuli
AU - Li Y
Technology ,
FAU - Zhu, Ye
AU - Zhu Y
AD - Department of Chemistry & Biochemistry, Stephenson Life Sciences Research
Center,
University of Oklahoma , Norman, Oklahoma 73019, United States.
FAU - Zeng, Lei
AU - Zeng L
Technology ,
FAU - Gao, Huichang
AU - Gao H
Technology ,
FAU - Yuan, Bo
AU - Yuan B
Technology ,
FAU - Chen, Xiaofeng
AU - Chen X
Technology ,
FAU - Mao, Chuanbin
AU - Mao C
AD - Department of Chemistry & Biochemistry, Stephenson Life Sciences Research
Center,
University of Oklahoma , Norman, Oklahoma 73019, United States.
AD - School of Materials Science and Engineering, Zhejiang University , Hangzhou
310027,
China.
LA - eng
GR - R21 EB015190/EB/NIBIB NIH HHS/United States
GR - EB015190/EB/NIBIB NIH HHS/United States
DEP - 20151012
JID - 101504991
RN - 0 (Collagen Type I)
RN - 0 (Polyesters)
RN - 0 (Polymers)
SB - IM
MH - Cell Line
MH - Cell Movement/*drug effects
MH - Cell Shape/drug effects
MH - Collagen Type I/metabolism
MH - Fluorescent Antibody Technique
MH - Humans
MH - Mesenchymal Stem Cells/cytology/drug effects
MH - *Models, Biological
MH - Polyesters/pharmacology
MH - Polymers/chemistry/*pharmacology
MH - Real-Time Polymerase Chain Reaction
PMC - PMC4934131
MID - NIHMS796995
OTO - NOTNLM
OT - bone
OT - collective cell migration
OT - implants
OT - microgrooved topography
COIS- Notes The authors declare no competing financial interest.
EDAT- 2015/10/13 06:00
MHDA- 2016/09/07 06:00
CRDT- 2015/10/13 06:00
PHST- 2015/10/13 06:00 [entrez]
PHST- 2015/10/13 06:00 [pubmed]
PHST- 2016/09/07 06:00 [medline]
AID - 10.1021/acsami.5b07976 [doi]
PST - ppublish
SO - ACS Appl Mater Interfaces. 2015 Oct 21;7(41):23336-45. doi:
10.1021/acsami.5b07976.
Epub 2015 Oct 12.
PMID- 23413842
OWN - NLM
STAT- MEDLINE
DCOM- 20130806
LR - 20161125
IS - 0218-8104 (Linking)
VI - 18
IP - 1
DP - 2013
TI - Effects on bone union and prevention of tendon adhesion by new porous anti-
adhesive
poly L-lactide-co-ε-caprolactone membrane in a rabbit model.
PG - 1-10
LID - 10.1142/S0218810413500019 [doi]
AB - To prevent adhesion between the tendon and the plate in hand surgery, a
porous poly
L-lactide-co-ε-caprolactone (P(LA/CL)) membrane was developed as a
biologically
absorbable anti-adhesion material. Our study aims to confirm the efficacy of
this
new P(LA/CL) membrane and its influences on the osteotomy site by performing
a
fundamental experiment assessing the possibility of clinical application. We
prepared a rabbit model of tendon-plate adhesion, and evaluated the efficacy
of the
P(LA/CL) membrane to pullout tendon strength, to be scored in terms of
macroscopic,
pathological results. Another rabbit model of osteotomy was prepared to
confirm the
P(LA/CL) membrane influences on bone union by radiological, mechanical and
pathological evaluation. The result showed to be significantly lower adhesion
in the
P(LA/CL) membrane group than in the control group. Also there were no
differences
between the P(LA/CL) membrane group and control group in the results for bone
union.
FAU - Sato, Takuya
AU - Sato T
AD - Department of Orthopaedic Surgery, St. Marianna University School of
Medicine,
Kanagawa, Japan. satotaku@kg8.so-net.ne.jp
FAU - Shimizu, Hiroyuki
AU - Shimizu H
FAU - Beppu, Moroe
AU - Beppu M
FAU - Takagi, Masayuki
AU - Takagi M
LA - eng
PL - Singapore
TA - Hand Surg
JT - Hand surgery : an international journal devoted to hand and upper limb
surgery and
related research : journal of the Asia-Pacific Federation of Societies for
Surgery
of the Hand
JID - 9602613
RN - 0 (Caproates)
RN - 0 (Lactones)
RN - 0 (Membranes, Artificial)
RN - 56RE988L1R (caprolactone)
SB - IM
MH - Animals
MH - *Caproates
MH - Fractures, Bone/pathology/*surgery
MH - *Lactones
MH - *Membranes, Artificial
MH - Orthopedic Procedures/methods
MH - Rabbits
MH - Tendon Injuries/pathology/*surgery
MH - Tendons/*pathology/surgery
MH - Tissue Adhesions/etiology/*prevention & control
EDAT- 2013/02/19 06:00
MHDA- 2013/08/07 06:00
CRDT- 2013/02/19 06:00
PHST- 2013/02/19 06:00 [entrez]
PHST- 2013/02/19 06:00 [pubmed]
PHST- 2013/08/07 06:00 [medline]
AID - 10.1142/S0218810413500019 [doi]
PST - ppublish
SO - Hand Surg. 2013;18(1):1-10. doi: 10.1142/S0218810413500019.
PMID- 24909139
OWN - NLM
STAT- MEDLINE
DCOM- 20150109
LR - 20201209
IS - 0021-9541 (Linking)
VI - 230
IP - 1
DP - 2015 Jan
TI - EGFL7 is expressed in bone microenvironment and promotes angiogenesis via
ERK,
STAT3, and integrin signaling cascades.
PG - 82-94
LID - 10.1002/jcp.24684 [doi]
AB - Angiogenesis plays a pivotal role in bone formation, remodeling, and fracture
healing. The regulation of angiogenesis in the bone microenvironment is

highly
complex and orchestrated by intercellular communication between bone cells
and
endothelial cells. Here, we report that EGF-like domain 7 (EGFL7), a member
of the
epidermal growth factor (EGF) repeat protein superfamily is expressed in both
the
osteoclast and osteoblast lineages, and promotes endothelial cell activities.
Addition of exogenous recombinant EGFL7 potentiates SVEC (simian virus

40-transformed mouse microvascular endothelial cell line) cell migration and
tube-like structure formation in vitro. Moreover, recombinant EGFL7 promotes
angiogenesis featuring web-like structures in ex vivo fetal mouse metatarsal
angiogenesis assay. We show that recombinant EGFL7 induces phosphorylation of
extracellular signal-regulated kinase 1/2 (ERK1/2), signal transducer and

activator
of transcription 3 (STAT3), and focal adhesion kinase (FAK) in SVEC cells.
Inhibition of ERK1/2 and STAT3 signaling impairs EGFL7-induced endothelial
cell
migration, and angiogenesis in fetal mouse metatarsal explants. Bioinformatic
analyses indicate that EGFL7 contains a conserved RGD/QGD motif and EGFL7-
induced
endothelial cell migration is significantly reduced in the presence of RGD
peptides.
Moreover, EGFL7 gene expression is significantly upregulated during growth
plate
injury repair. Together, these results demonstrate that EGFL7 expressed by
bone
cells regulates endothelial cell activities through integrin-mediated
signaling.
This study highlights the important role that EGFL7, like EGFL6, expressed in
bone
microenvironment plays in the regulation of angiogenesis in bone.
AU - Chim SM
Nedlands, WA, Australia.

FAU - Kuek, Vincent
AU - Kuek V
FAU - Chow, Siu To
AU - Chow ST
FAU - Lim, Bay Sie
AU - Lim BS
FAU - Tickner, Jennifer
AU - Tickner J
FAU - Zhao, Jinmin
AU - Zhao J
FAU - Chung, Rosa
AU - Chung R
FAU - Su, Yu-Wen
AU - Su YW
FAU - Zhang, Ge
AU - Zhang G
FAU - Erber, Wendy
AU - Erber W
FAU - Xian, Cory J
AU - Xian CJ
FAU - Rosen, Vicki
AU - Rosen V
FAU - Xu, Jiake
AU - Xu J
LA - eng
PL - United States
TA - J Cell Physiol
JT - Journal of cellular physiology
JID - 0050222
RN - 0 (Calcium-Binding Proteins)
RN - 0 (EGF Family of Proteins)
RN - 0 (Egfl7 protein, mouse)
RN - 0 (Integrins)
RN - 0 (Proteins)
RN - 0 (STAT3 Transcription Factor)
RN - 0 (Stat3 protein, rat)
RN - 104982-03-8 (Osteocalcin)
RN - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB - IM
MH - Animals
MH - Bone and Bones/blood supply/cytology
MH - Calcium-Binding Proteins
MH - EGF Family of Proteins
MH - Endothelial Cells/*metabolism
MH - Extracellular Signal-Regulated MAP Kinases/antagonists &
inhibitors/*metabolism
MH - Focal Adhesion Protein-Tyrosine Kinases/metabolism
MH - Growth Plate/metabolism
MH - Integrins/*metabolism
MH - Male
MH - Mice
MH - Neovascularization, Physiologic/*drug effects
MH - Osteoblasts/metabolism
MH - Osteocalcin/biosynthesis
MH - Osteoclasts/metabolism
MH - Phosphorylation/drug effects
MH - Proteins/*metabolism/pharmacology
MH - Rats
MH - STAT3 Transcription Factor/antagonists & inhibitors/*metabolism
MH - Salter-Harris Fractures
MH - Vascular Endothelial Growth Factor A/biosynthesis
EDAT- 2014/06/10 06:00
MHDA- 2015/01/13 06:00
CRDT- 2014/06/10 06:00
PHST- 2013/08/09 00:00 [received]
PHST- 2014/05/21 00:00 [accepted]
PHST- 2014/06/10 06:00 [entrez]
PHST- 2014/06/10 06:00 [pubmed]
PHST- 2015/01/13 06:00 [medline]
AID - 10.1002/jcp.24684 [doi]
PST - ppublish
SO - J Cell Physiol. 2015 Jan;230(1):82-94. doi: 10.1002/jcp.24684.
PMID- 28962655
OWN - NLM
STAT- MEDLINE
DCOM- 20180523
LR - 20181202
IS - 1757-6512 (Linking)
VI - 8
IP - 1
DP - 2017 Sep 29
TI - A unique heterologous fibrin sealant (HFS) as a candidate biological scaffold
for
mesenchymal stem cells in osteoporotic rats.
PG - 205
LID - 10.1186/s13287-017-0654-7 [doi]
LID - 205
AB - BACKGROUND: The injection of mesenchymal stem cells (MSCs) directly into the
bone of
osteoporotic (OP) patients for rapid recovery has been studied worldwide.
Scaffolds
associated with MSCs are used to maintain and avoid cell loss after
application. A
unique heterologous fibrin sealant (HFS) derived from snake venom was
evaluated for
the cytotoxicity of its main components and as a three-dimensional biological
scaffold for MSCs to repair a critical femur defect in osteoporotic rats.

METHODS:
The cytotoxicity of HFS was assessed using a
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay
and
transmission electron microscopy. The cells were cultured, characterized by
flow
cytometry and differentiated into the osteogenic lineage. Two-month-old rats
underwent ovariectomy to induce OP. After 3 months, a 5 mm critical bone
defect was
made in the distal end of the rat femurs and filled with HFS; HFS + MSCs; and
HFS + MSCs D (differentiated into the osteogenic lineage) to evaluate the

effects.
An injury control group (injury and no treatment) and blank control group (no
injury
and no treatment) were also included. The animals were observed at days 14
and 28 by
microtomographic (micro-CT) analyses, histologic and biochemical analysis, as
well
as scanning electron microscopy. RESULTS: The results revealed that one of
the
compounds of HFS, the thrombin-like enzyme extracted from snake venom, had no
cytotoxic effects on the MSCs. OP was successfully induced, as demonstrated

by the
significant differences in the levels of 17β-estradiol, Micro-CT analyses and
alkaline phosphatase between the ovariectomized (OVX) and non-ovariectomized

(NOVX)
groups. The histological data revealed that at 14 days after surgery in both
the OVX
and NOVX animals, the HFS + CTMs and HFS + CTMsD showed a higher formation of
bone
cells at the site in relation to the control group (without treatment).
Collagen
formation was evidenced through bone neoformation in all treated and control
groups.
No morphological differences in the femurs of the NOVX and OVX animals were
observed
after the surgical procedure. Scanning electron microscopy (SEM) confirmed
the
histological analysis. CONCLUSIONS: The new HFS composed of two non-toxic
components
for MSCs showed capacity to promote the recovery of the bone lesions in OVX
and NOVX
animals at 14 days after surgery. In addition, the HFS enabled the
differentiation
of MSCs into MSCs D in the group treated with HFS + MSCs. Using the MSCs
and/or MSCs
D together with this biopharmaceutical could potentially enable significant
advances
in the treatment of osteoporotic fractures. Future clinical trials will be
necessary
to confirm these results.
FAU - Orsi, Patrícia Rodrigues
AU - Orsi PR
AD - Center for the Study of Venoms and Venomous Animals (CEVAP), UNESP -
Universidade
Estadual Paulista, Botucatu, SP, Brazil.
AD - Botucatu Medical School, UNESP - Universidade Estadual Paulista, Botucatu,
SP,
Brazil.
FAU - Landim-Alvarenga, Fernanda Cruz
AU - Landim-Alvarenga FC
AD - College of Veterinary Medicine and Animal Husbandry (FMVZ), UNESP - Univ
Estadual
Paulista, Botucatu, SP, Brazil.
FAU - Justulin, Luis Antônio Jr
AU - Justulin LA Jr
AD - Botucatu Biosciences Institute, UNESP - Universidade Estadual Paulista,
Botucatu,
SP, Brazil.
FAU - Kaneno, Ramon
AU - Kaneno R
Botucatu,
SP, Brazil.
FAU - de Assis Golim, Marjorie
AU - de Assis Golim M
SP,
Brazil.
FAU - Dos Santos, Daniela Carvalho
AU - Dos Santos DC
Botucatu,
SP, Brazil.
FAU - Creste, Camila Fernanda Zorzella
AU - Creste CFZ
Universidade
SP,
Brazil.
FAU - Oba, Eunice
AU - Oba E
Estadual
FAU - Maia, Leandro
AU - Maia L
Estadual
FAU - Barraviera, Benedito
AU - Barraviera B
Universidade
SP,
Brazil.
FAU - Ferreira, Rui Seabra Jr
AU - Ferreira RS Jr
Universidade
Estadual Paulista, Botucatu, SP, Brazil. rseabra@cevap.unesp.br.
SP,
Brazil. rseabra@cevap.unesp.br.
LA - eng
DEP - 20170929
TA - Stem Cell Res Ther
JT - Stem cell research & therapy
JID - 101527581
SB - IM
MH - Animals
MH - Female
MH - Fibrin Tissue Adhesive/*adverse effects
MH - Humans
MH - Mesenchymal Stem Cells/cytology/*drug effects/metabolism
MH - Osteoporosis, Postmenopausal/*therapy
MH - Rats
MH - Rats, Wistar
MH - Tissue Scaffolds/chemistry
PMC - PMC5622505
OTO - NOTNLM
OT - *Cytotoxicity
OT - *Fibrin sealant
OT - *Fibrinogen
OT - *Osteoporosis
OT - *Snake venom
COIS- ETHICS APPROVAL: The animals were handled and the surgical procedures were
conducted
in accordance with the Brazilian College for Animal Experimentation
guidelines, and
the protocols were approved by the university’s Ethics Committee in
Experimental
Animal Use (protocol number 998/2013). CONSENT FOR PUBLICATION: Not
applicable.
COMPETING INTERESTS: The authors declare that they have no competing
interests.
PUBLISHER’S NOTE: Springer Nature remains neutral with regard to
jurisdictional
claims in published maps and institutional affiliations.
EDAT- 2017/10/01 06:00
MHDA- 2018/05/24 06:00
CRDT- 2017/10/01 06:00
PHST- 2017/05/16 00:00 [received]
PHST- 2017/08/30 00:00 [accepted]
PHST- 2017/08/27 00:00 [revised]
PHST- 2017/10/01 06:00 [entrez]
PHST- 2017/10/01 06:00 [pubmed]
PHST- 2018/05/24 06:00 [medline]
AID - 10.1186/s13287-017-0654-7 [pii]
AID - 654 [pii]
AID - 10.1186/s13287-017-0654-7 [doi]
PST - epublish
SO - Stem Cell Res Ther. 2017 Sep 29;8(1):205. doi: 10.1186/s13287-017-0654-7.
PMID- 21485564
OWN - NLM
STAT- MEDLINE
DCOM- 20110518
LR - 20161125
IS - 1003-0034 (Print)
IS - 1003-0034 (Linking)
VI - 24
IP - 3
DP - 2011 Mar
TI - [Analysis and prevention of the complications after treatment of metacarpal
and
phalangeal fractures with internal fixation].
PG - 199-201
AB - OBJECTIVE: To retrospective analysis the complications after treatment of
metacarpal
and phalangeal fractures with internal fixation, and propose measures to
prevent or
reduce surgical complications. METHODS: From July 2007 to October 2009, 342
patients
with metacarpal and phalangeal fractures were treated with internal
fixation,including 203 males and 139 females with an average age of 30.4
years old
ranging from 18 to 56 years. There were 217 right hands and 125 left hands,
38 cases
of the first metacarpal fracture, 47 cases of the second metacarpal fracture,
52
cases of the third metacarpal fracture, 40 cases of the forth metacarpal
fracture,
39 cases of the fifth metacarpal fracture, 43 cases of the proximal
phalangeal
fractures, 52 cases of the middle phalangeal fractures, and 48 case of the
distal
phalangeal fractures. The fractures were fixed with K-wires in 129 patients,
mini
plates in 153 cases, screws in 48 cases, wires in 12 cases. RESULTS: All 324
patients were followed up for 3 to 15 months (averaged 8.5 months) and
complications
occurred in 74 patients (22.84%). The main complication was unhealthy wound
healing
in 24 patients (7.4%), others was adhesion of tendon in 54 patients (16.67%),
malunion in 34 patients (10.49%), nonunion in 13 patients (4.01%) and

shortening of
metacarpal and phalangeal in 21 patients (7.41%). CONCLUSION: The important
reasons
responsible for complications are iatrogenic factors such as unsuitable
implant
selection, unskillful surgical technique and improper post-operative
functional
rehabilitation.
FAU - Yan, Yu-ming
AU - Yan YM
AD - Department of Hand Surgery, the Second Hospital of Zhenhai, Ningbo, Zhejiang,
China.
yanyuming2003@163.com
FAU - Zhang, Wei-ping
AU - Zhang WP
FAU - Liao, Yong
AU - Liao Y
FAU - Weng, Ze-fu
AU - Weng ZF
FAU - Ren, Wei-jie
AU - Ren WJ
FAU - Lin, Jun
AU - Lin J
FAU - Tang, Xian-ao
AU - Tang XA
LA - chi
PL - China
TA - Zhongguo Gu Shang
JT - Zhongguo gu shang = China journal of orthopaedics and traumatology
JID - 9815790
SB - IM
MH - Adolescent
MH - Adult
MH - Female
MH - Finger Phalanges/diagnostic imaging/*injuries/physiopathology/surgery
MH - Fracture Fixation, Internal/*adverse effects
MH - Fractures, Bone/diagnostic imaging/physiopathology/*surgery
MH - Humans
MH - Male
MH - Metacarpal Bones/diagnostic imaging/*injuries/physiopathology/surgery
MH - Middle Aged
MH - Recovery of Function
MH - Young Adult
EDAT- 2011/04/14 06:00
MHDA- 2011/05/19 06:00
CRDT- 2011/04/14 06:00
PHST- 2011/04/14 06:00 [entrez]
PHST- 2011/04/14 06:00 [pubmed]
PHST- 2011/05/19 06:00 [medline]
PST - ppublish
SO - Zhongguo Gu Shang. 2011 Mar;24(3):199-201.
PMID- 21531721
OWN - NLM
STAT- MEDLINE
DCOM- 20110830
LR - 20210205
IS - 0021-9258 (Print)
IS - 0021-9258 (Linking)
VI - 286
IP - 25
DP - 2011 Jun 24
TI - EGFL6 promotes endothelial cell migration and angiogenesis through the
activation of
extracellular signal-regulated kinase.
PG - 22035-46
LID - 10.1074/jbc.M110.187633 [doi]
AB - Angiogenesis is required for bone development, growth, and repair. It is
influenced
by the local bone environment that involves cross-talks between endothelial
cells
and adjacent bone cells. However, data regarding factors that directly
contribute to
angiogenesis by bone cells remain poorly understood. Here, we report that
EGFL6, a
member of the epidermal growth factor (EGF) repeat superfamily proteins,
induces
angiogenesis by a paracrine mechanism in which EGFL6 is expressed in
osteoblastic-like cells but promotes migration and angiogenesis of
endothelial
cells. Co-immunoprecipitation assays revealed that EGFL6 is secreted in
culture
medium as a homodimer protein. Using scratch wound healing and transwell
assays, we
found that conditioned medium containing EGFL6 potentiates SVEC (a simian
virus
40-transformed mouse microvascular endothelial cell line) endothelial cell
migration. In addition, EGFL6 promotes the endothelial cell tube-like
structure
formation in Matrigel assays and angiogenesis in a chick embryo
chorioallantoic
membrane. Furthermore, we show that EGFL6 recombinant protein induces
phosphorylation of ERK in SVEC endothelial cells. Inhibition of ERK impaired
EGFL6-induced ERK activation and endothelial cell migration. Together, these
results
demonstrate, for the first time, that osteoblastic-like cells express EGFL6
that is
capable of promoting endothelial cell migration and angiogenesis via ERK
activation.
Thus, the EGLF6 mediates a paracrine mechanism of cross-talk between vascular
endothelial cells and osteoblasts and might offer an important new target for
the
potential treatment of bone diseases, including osteonecrosis, osteoporosis,
and
fracture healing.
AU - Chim SM
AD - School of Pathology and Laboratory Medicine, The Universityof Western
Australia,
Western Australia 6009, Australia
FAU - Qin, An
AU - Qin A
FAU - Tickner, Jennifer
AU - Tickner J
FAU - Pavlos, Nathan
AU - Pavlos N
FAU - Davey, Tamara
AU - Davey T
FAU - Wang, Hao
AU - Wang H
FAU - Guo, Yajun
AU - Guo Y
FAU - Zheng, Ming Hao
AU - Zheng MH
FAU - Xu, Jiake
AU - Xu J
LA - eng
DEP - 20110429
TA - J Biol Chem
JT - The Journal of biological chemistry
JID - 2985121R
RN - 0 (Calcium-Binding Proteins)
RN - 0 (Egfl6 protein, mouse)
RN - 0 (Glycoproteins)
RN - 0 (Neoplasm Proteins)
RN - 0 (Oligopeptides)
RN - 0 (Peptides)
RN - 0 (RNA, Messenger)
RN - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB - IM
MH - Animals
MH - Bone and Bones/blood supply
MH - COS Cells
MH - Calcium-Binding Proteins
MH - Cell Adhesion Molecules
MH - Cell Line
MH - *Cell Movement/drug effects
MH - Chick Embryo
MH - Chlorocebus aethiops
MH - Chorioallantoic Membrane/metabolism
MH - Endothelial Cells/*cytology/drug effects/*metabolism
MH - Enzyme Activation/drug effects
MH - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH - Gene Expression Regulation/drug effects
MH - Glycoproteins/chemistry/genetics/*metabolism
MH - Humans
MH - MAP Kinase Signaling System/drug effects
MH - Mice
MH - Neoplasm Proteins/chemistry/genetics/*metabolism
MH - *Neovascularization, Physiologic/drug effects
MH - Oligopeptides/pharmacology
MH - Osteoclasts/cytology
MH - Paracrine Communication/drug effects
MH - Peptides/chemistry/genetics/*metabolism
MH - Protein Multimerization/drug effects
MH - Protein Structure, Quaternary
MH - RNA, Messenger/genetics/metabolism
PMC - PMC3121348
EDAT- 2011/05/03 06:00
MHDA- 2011/08/31 06:00
CRDT- 2011/05/03 06:00
PHST- 2011/05/03 06:00 [entrez]
PHST- 2011/05/03 06:00 [pubmed]
PHST- 2011/08/31 06:00 [medline]
AID - S0021-9258(19)48911-4 [pii]
AID - M110.187633 [pii]
AID - 10.1074/jbc.M110.187633 [doi]
PST - ppublish
SO - J Biol Chem. 2011 Jun 24;286(25):22035-46. doi: 10.1074/jbc.M110.187633. Epub
2011
Apr 29.
PMID- 29806317
OWN - NLM
STAT- MEDLINE
DCOM- 20180628
LR - 20190401
IS - 1002-1892 (Print)
IS - 1002-1892 (Linking)
VI - 31
IP - 10
DP - 2017 Oct 15
TI - [Effectiveness comparison between ultrasound-guided and C-arm-guided in
closed
reduction and pinning for treatment of metacarpophalangeal fractures].
PG - 1179-1183
LID - 10.7507/1002-1892.201705037 [doi]
AB - OBJECTIVE: To compare the effectiveness between ultrasound-guided and C-arm-
guided
in closed reduction and pinning for the treatment of metacarpophalangeal
fractures.
METHODS: The clinical data of 30 patients with metacarpophalangeal fractures
between
October 2015 and November 2016 were retrospectively analyzed. According to
different
treatments, the patients were divided into ultrasound group (using
ultrasound-guided
closed reduction and pinning, n=15) and C-arm group (using C-arm-guided
closed
reduction and pinning, n=15). There was no significant difference in gender,
age,
disease duration, causes of injury, injured finger, location of injury
finger,
fracture classification between 2 groups ( P>0.05). The status and success
rate of
reduction were compared (excellent, good, and acceptable grades could be
regarded as
the successful reduction). The operation time, intraoperative fluoroscopy
times, and
fracture healing time were recorded. And the postoperative functional
recovery was
evaluated according to the total active movement (TAM) by the standard
functional
evaluation issued by Hand Surgery Association of Chinese Medical Association.
RESULTS: The operation time of ultrasound group was longer than C-arm group,
and the
intraoperative fluoroscopy times was less than C-arm group, all showing
significant
differences ( P<0.05). There was no signifi cant difference in the grade and
the
success rate of reduction between 2 groups ( P>0.05). All the patients were
followed
up 6-18 months (mean, 10 months), without malunion, joint stiffness, tendon
adhesions, and other complications. There was also no significant difference
in the
fracture healing time, the grade of TAM, and the excellent and good rate of
TAM
between 2 groups ( P>0.05). CONCLUSION: The treatment of ultrasound-guided
closed
reduction and pinning for metacarpophalangeal fractures is effective, which
is a
feasible auxiliary method of closed reduction and fixation for fracture. And
less
fluoroscopy can reduce the radiation damage of operation.
FAU - Wang, Lei
AU - Wang L
AD - Hunan University of Chinese Medicine, Changsha Hunan, 410000, P.R.China.
FAU - Wang, Xiaohui
AU - Wang X
AD - Department of Hand Micro Orthopedics, Luoyang Orthopedic Hospital of Henan
Province
(Orthopedic Hospital of Henan Province), Luoyang Henan, 471002,
P.R.China.963099784@qq.com.
FAU - Li, Wuyin
AU - Li W
AD - Department of Hand Micro Orthopedics, Luoyang Orthopedic Hospital of Henan
Province
(Orthopedic Hospital of Henan Province), Luoyang Henan, 471002, P.R.China.
LA - chi
PL - China
zazhi =
JID - 9425194
SB - IM
MH - Finger Injuries/*surgery
MH - *Fracture Fixation, Intramedullary
MH - Humans
OTO - NOTNLM
OT - *Ultrasonography
OT - *internal fixation
OT - *metacarpophalangeal fracture
EDAT- 2018/05/29 06:00
MHDA- 2018/06/29 06:00
CRDT- 2018/05/29 06:00
PHST- 2018/05/29 06:00 [entrez]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2018/06/29 06:00 [medline]
AID - 10.7507/1002-1892.201705037 [doi]
PST - ppublish
SO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2017 Oct 15;31(10):1179-1183. doi:
10.7507/1002-1892.201705037.
PMID- 20664370
OWN - NLM
STAT- MEDLINE
DCOM- 20110204
LR - 20181201
IS - 0022-5282 (Linking)
VI - 70
IP - 1
DP - 2011 Jan
TI - Combination of guided osteogenesis with autologous platelet-rich fibrin glue
and
mesenchymal stem cell for mandibular reconstruction.
PG - 228-37
LID - 10.1097/TA.0b013e3181e12b56 [doi]
AB - BACKGROUND: This study examined whether a combination of autologous platelet-
rich
fibrin glue (PRFG) with mesenchymal stem cells (MSCs) and MEDPOR as guided
tissue
regeneration (GTR) could act as an osteogenic substitute and whether this
treatment
yields faster new bone formation than MEDPOR alone or PRFG plus MSC.
MATERIAL: MSCs
were harvested and isolated from the bone marrow of dog ilium. Full-thickness
bony
defects (1.5×1.5 cm) were created in the bilateral mandible angles of the
dog.
Treatments for bone defect in each group were as follows: group I (n=4),
MEDPOR
sheet as GTR and autologous PRFG/MSCs admixtures; group II (n=4), autologous
PRFG/MSCs admixtures; group III (n=4), MEDPOR sheet as GTR; and group IV
(n=4),
control (empty defect). The percentage of new bone regeneration in
computerized
tomography at 2 months and 4 months was calculated by Analyze version 7.0
software.
The mandibles were harvested from all specimens at 4 months, and the grafted
sites
were evaluated by gross, histologic, and X-ray examination. RESULTS: By
radiographic
analysis at 16 weeks posttransplantation, it was shown that an average of
72.8%±8.02% new bone formation in group I, 53.34%±6.87% in group II, 26.58%
±6.41% in
group III, and 15.14%±2.37% in group IV. Histologic examination revealed that
the
defect was repaired by typical bone tissue in groups I and II, whereas only
minimal
bone formation with fibrous connection was observed in the groups III and IV
group.
Besides, muscle incarceration was found in groups II and IV without MEDPOR as
GTR.
CONCLUSION: Autologous PRFG plus osteoinduced MSCs have good potential for
bone
regeneration. In combination with MEDPOR as GTR, bone regeneration is
enhanced by
preventing soft tissue ingrowth hindering bone regeneration.
FAU - Liao, Han-Tsung
AU - Liao HT
AD - Division of Traumatic Plastic Surgery, Department of Plastic and
Reconstructive
Surgery, Chang Gung Memorial Hospitals, Chang Gung University College of
Medicine,
Taipei, Taiwan.
FAU - Chen, Chien-Tzung
AU - Chen CT
FAU - Chen, Chih-Hao
AU - Chen CH
FAU - Chen, Jyh-Ping
AU - Chen JP
FAU - Tsai, Jui-Che
AU - Tsai JC
LA - eng
PL - United States
TA - J Trauma
JT - The Journal of trauma
JID - 0376373
SB - AIM
SB - IM
MH - Animals
MH - Blood Platelets/physiology
MH - Dogs
MH - Mandible/diagnostic imaging/growth & development/physiology/*surgery
MH - Mandibular Fractures/surgery
MH - Mesenchymal Stem Cells/*physiology
EDAT- 2010/07/29 06:00
MHDA- 2011/02/05 06:00
CRDT- 2010/07/29 06:00
PHST- 2010/07/29 06:00 [entrez]
PHST- 2010/07/29 06:00 [pubmed]
PHST- 2011/02/05 06:00 [medline]
AID - 10.1097/TA.0b013e3181e12b56 [doi]
PST - ppublish
SO - J Trauma. 2011 Jan;70(1):228-37. doi: 10.1097/TA.0b013e3181e12b56.
PMID- 26106926
OWN - NLM
STAT- MEDLINE
DCOM- 20160422
LR - 20161125
IS - 1748-6041 (Linking)
VI - 10
IP - 3
DP - 2015 Jun 24
TI - Membrane-reinforced three-dimensional electrospun silk fibroin scaffolds for
bone
tissue engineering.
PG - 035011
LID - 10.1088/1748-6041/10/3/035011 [doi]
AB - Electrospun silk fibroin (SF) scaffolds have drawn much attention because of
their
resemblance to natural tissue architecture such as extracellular matrix, and
the
biocompatibility of SF as a candidate material to replace collagen. However,
electrospun scaffolds lack the physical integrity of bone tissue scaffolds,
which
require resistance to mechanical loadings. In this work, we propose
membrane-reinforced electrospun SF scaffolds by a serial process of
electrospinning
and freeze-drying of SF solutions in two different solvents: formic acid and
water,
respectively. After wet electrospinning followed by replacement of methanol
with
water, SF nanofibers dispersed in water were mixed with aqueous SF solution.
Freeze-drying of the mixed solution resulted in 3D membrane-connected SF
nanofibrous
scaffolds (SF scaffolds) with a thickness of a few centimeters. We
demonstrated that
the SF concentration of aqueous SF solution controlled the degree of membrane
reinforcement between nanofibers. It was also shown that both increase in

degree of
membrane reinforcement and inclusion of hydroxyapatite (HAP) nanoparticles
resulted
in higher resistance to compressive loadings of the SF scaffolds. Culture of
human
osteoblasts on collagen, SF, and SF-HAP scaffolds showed that both SF and SF-
HAP
scaffolds had biocompatibility and cell proliferation superior to that of the
collagen scaffolds. SF-HAP scaffolds with and without BMP-2 were used for in
vivo
studies for 4 and 8 weeks, and they showed enhanced bone tissue formation in
rat
calvarial defect models.
FAU - Yang, Sung Yeun
AU - Yang SY
AD - School of Mechanical Engineering, Yonsei University, Seoul 120-749, Korea.
FAU - Hwang, Tae Heon
AU - Hwang TH
FAU - Che, Lihua
AU - Che L
FAU - Oh, Jin Soo
AU - Oh JS
FAU - Ha, Yoon
AU - Ha Y
FAU - Ryu, WonHyoung
AU - Ryu W
LA - eng
DEP - 20150624
PL - England
TA - Biomed Mater
JID - 101285195
RN - 9007-76-5 (Fibroins)
SB - IM
MH - Animals
MH - Cell Adhesion
MH - Cell Line
MH - Cell Survival
MH - Durapatite/chemistry
MH - Fibroins/*chemistry
MH - Humans
MH - Male
MH - Nanofibers/chemistry
MH - Osteogenesis
MH - Porosity
MH - Rats
MH - Skull/diagnostic imaging/injuries/pathology
EDAT- 2015/06/25 06:00
MHDA- 2016/04/23 06:00
CRDT- 2015/06/25 06:00
PHST- 2015/06/25 06:00 [entrez]
PHST- 2015/06/25 06:00 [pubmed]
PHST- 2016/04/23 06:00 [medline]
AID - 10.1088/1748-6041/10/3/035011 [doi]
PST - epublish
SO - Biomed Mater. 2015 Jun 24;10(3):035011. doi: 10.1088/1748-6041/10/3/035011.
PMID- 21732280
OWN - NLM
STAT- MEDLINE
DCOM- 20111227
LR - 20191112
IS - 1473-2262 (Linking)
VI - 22
DP - 2011 Jul 6
TI - CD73 and CD29 concurrently mediate the mechanically induced decrease of
migratory
capacity of mesenchymal stromal cells.
PG - 26-42
AB - e assumption that mesenchymal stromal cell (MSC)-based-therapies are capable
of
augmenting physiological regeneration processes has fostered intensive basic
and
clinical research activities. However, to achieve sustained therapeutic
success in
vivo, not only the biological, but also the mechanical microenvironment of
MSCs
during these regeneration processes needs to be taken into account. This is
especially important for e.g., bone fracture repair, since MSCs present at
the
fracture site undergo significant biomechanical stimulation. This study has
therefore investigated cellular characteristics and the functional behaviour
of MSCs
in response to mechanical loading. Our results demonstrated a reduced
expression of
MSC surface markers CD73 (ecto-5'-nucleotidase) and CD29 (integrin β1) after
loading. On the functional level, loading led to a reduced migration of MSCs.
Both
effects persisted for a week after the removal of the loading stimulus.
Specific
inhibition of CD73/CD29 demonstrated their substrate dependent involvement in
MSC
migration after loading. These results were supported by scanning electron
microscopy images and phalloidin staining of actin filaments displaying less
cell
spreading, lamellipodia formation and actin accumulations. Moreover, focal
adhesion
kinase and Src-family kinases were identified as candidate downstream targets
of
CD73/CD29 that might contribute to the mechanically induced decrease in MSC
migration. These results suggest that MSC migration is controlled by
CD73/CD29,
which in turn are regulated by mechanical stimulation of cells. We therefore
speculate that MSCs migrate into the fracture site, become mechanically
entrapped,
and thereby accumulate to fulfil their regenerative functions.
FAU - Ode, A
AU - Ode A
AD - Julius Wolff Institute and Musculoskeletal Research Center Berlin,
Charité-Universitätsmedizin, Berlin, Germany.
FAU - Kopf, J
AU - Kopf J
FAU - Kurtz, A
AU - Kurtz A
FAU - Schmidt-Bleek, K
AU - Schmidt-Bleek K
FAU - Schrade, P
AU - Schrade P
FAU - Kolar, P
AU - Kolar P
FAU - Buttgereit, F
AU - Buttgereit F
FAU - Lehmann, K
AU - Lehmann K
FAU - Hutmacher, D W
AU - Hutmacher DW
FAU - Duda, G N
AU - Duda GN
FAU - Kasper, G
AU - Kasper G
LA - eng
DEP - 20110706
PL - Switzerland
TA - Eur Cell Mater
JT - European cells & materials
JID - 100973416
RN - 0 (GPI-Linked Proteins)
RN - 0 (Integrin beta1)
RN - EC 3.1.3.5 (5'-Nucleotidase)
RN - EC 3.1.3.5 (NT5E protein, human)
SB - IM
MH - 5'-Nucleotidase/*physiology
MH - *Biomechanical Phenomena
MH - *Cell Movement
MH - Fractures, Bone/therapy
MH - GPI-Linked Proteins/physiology
MH - Humans
MH - Integrin beta1/*physiology
MH - Mesenchymal Stem Cells/*cytology/physiology
MH - *Regeneration
MH - Wound Healing
EDAT- 2011/07/07 06:00
MHDA- 2011/12/28 06:00
CRDT- 2011/07/07 06:00
PHST- 2011/07/07 06:00 [entrez]
PHST- 2011/07/07 06:00 [pubmed]
PHST- 2011/12/28 06:00 [medline]
AID - vol022a03 [pii]
AID - 10.22203/ecm.v022a03 [doi]
PST - epublish
SO - Eur Cell Mater. 2011 Jul 6;22:26-42. doi: 10.22203/ecm.v022a03.
PMID- 28277417
OWN - NLM
STAT- MEDLINE
DCOM- 20180418
LR - 20181202
VI - 26
IP - 5
DP - 2017 Sep
TI - Comparison of healing intra-articular fracture of distal femur using a
Kirschner
wire and autologous fibrin glue in an animal model.
PG - 454-457
LID - 10.1097/BPB.0000000000000444 [doi]
AB - Considering different surgical techniques for the fixation of osteochondral
intra-articular fracture, the present study aimed to compare the efficacy of
autologous fibrin glue and Kirschner wire (KW) on an osteochondral fracture
in the
left lateral condyle of Dutch rabbits with a control group. After 6 weeks,
macroscopic and microscopic evaluation showed that autologous fibrin glue is
easier
and faster with a higher number of bone trabecula (P<0.05), whereas the
healing rate
and cellularity of the healing site were not different between the two groups
(KW
and glue). The use of autologous fibrin glue can be an alternative to KW
fixation in
the fixation of osteochondral fractures. LEVEL OF EVIDENCE: Level II.
FAU - Azarpira, Mohammad R
AU - Azarpira MR
AD - aDepartment of Orthopedics bBone and Joint Diseases Research Center
cTransplant
Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
FAU - Vazani, Kasra
AU - Vazani K
FAU - Ayatollahi, Maryam
AU - Ayatollahi M
FAU - Azarpira, Negar
AU - Azarpira N
FAU - Kaviani, Maryam
AU - Kaviani M
LA - eng
PL - United States
TA - J Pediatr Orthop B
JT - Journal of pediatric orthopedics. Part B
JID - 9300904
SB - IM
MH - Animals
MH - Bone Wires/*statistics & numerical data
MH - Cartilage, Articular/diagnostic imaging/surgery
MH - Femur/diagnostic imaging/*injuries/*surgery
MH - Fibrin Tissue Adhesive/*administration & dosage
MH - *Fracture Healing/physiology
MH - Intra-Articular Fractures/diagnostic imaging/*surgery
MH - Male
MH - Models, Animal
MH - Rabbits
EDAT- 2017/03/10 06:00
MHDA- 2018/04/19 06:00
CRDT- 2017/03/10 06:00
PHST- 2017/03/10 06:00 [pubmed]
PHST- 2018/04/19 06:00 [medline]
PHST- 2017/03/10 06:00 [entrez]
AID - 10.1097/BPB.0000000000000444 [doi]
PST - ppublish
SO - J Pediatr Orthop B. 2017 Sep;26(5):454-457. doi:
10.1097/BPB.0000000000000444.
PMID- 30839472
OWN - NLM
STAT- MEDLINE
DCOM- 20190501
LR - 20200511
IS - 0271-6798 (Linking)
VI - 39
IP - 4
DP - 2019 Apr
TI - Extensor Tendon Injury Associated With Dorsal Entry Flexible Nailing of
Radial Shaft
Fractures in Children: A Report of 5 New Cases and Review of the Literature.
PG - 163-168
LID - 10.1097/BPO.0000000000000897 [doi]
AB - BACKGROUND: Extensor pollicis longus (EPL) tendon injury following the dorsal
approach to elastic stable intramedullary nailing (ESIN) of the radius has

been
reported in a growing number of cases in the literature. This study includes
5 new
cases from our institution as well as a comprehensive review of previously
reported
cases from the literature. METHODS: We conducted a retrospective chart review
of all
patients undergoing ESIN between January 1, 2004 and December 31, 2013 at our
institution. Those patients with an EPL injury or rupture were identified and
clinical data collected included operative technique, diagnosis, treatment,

and
outcomes data. In addition, we performed a systematic review of the
literature using
Pubmed MEDLINE database, the Chochrane database, Scopus, Web of Science, and
Embase.
A total of 28 cases of EPL injury following ESIN of the radius were
identified in
the literature and the relevant data were extracted from those studies.
RESULTS: All
33 pediatric cases of EPL tendon injury occurred after entry to the radial
canal was
obtained by the dorsal approach to ESIN. EPL injury was diagnosed an average
of 10
weeks following the index procedure. Extensor indicis pollicis to EPL
transfer was
performed in 13 patients, tendon release/lysis of adhesions in 5, EPL repair
in 2,
EPL graft reconstruction from palmaris longus tendon in 1, 3 patients refused
further intervention, and treatment was unreported in 7 cases. By 12-month

follow-up, all operatively treated patients had a good functional outcome
with near
anatomic extension at the thumb interphalangeal joint, no pain, and no
further
complication. CONCLUSIONS: EPL tendon injury was found to be a complication
unique
to the dorsal entry approach for ESIN of the radius. The lateral approach
appears to
offer a safer alternative with regard to the EPL tendon. We suggest that
physicians
consider the risk of EPL tendon injury when planning for ESIN of the radius,
and
make an effort to avoid direct injury when using a dorsal approach. LEVEL OF
EVIDENCE: Level III-therapeutic.
FAU - Murphy, Hamadi A
AU - Murphy HA
AD - University of Cincinnati College of Medicine.
FAU - Jain, Viral V
AU - Jain VV
AD - Division of Pediatric Orthopaedic Surgery, Cincinnati Children's Hospital
Medical
Center, Cincinnati, OH.
FAU - Parikh, Shital N
AU - Parikh SN
Medical
FAU - Wall, Eric J
AU - Wall EJ
Medical
FAU - Cornwall, Roger
AU - Cornwall R
Medical
FAU - Mehlman, Charles T
AU - Mehlman CT
Medical
LA - eng
PT - Case Reports
PT - Review
PL - United States
JID - 8109053
SB - IM
MH - Adolescent
MH - Bone Nails/*adverse effects
MH - Child
MH - Female
MH - Fracture Fixation, Intramedullary/*adverse effects
MH - Humans
MH - Male
MH - Radius Fractures/diagnosis/*surgery
MH - Tendon Injuries/diagnosis/*etiology/surgery
MH - Tendon Transfer/*methods
EDAT- 2019/03/07 06:00
MHDA- 2019/05/02 06:00
CRDT- 2019/03/07 06:00
PHST- 2019/03/07 06:00 [entrez]
PHST- 2019/03/07 06:00 [pubmed]
PHST- 2019/05/02 06:00 [medline]
AID - 01241398-201904000-00002 [pii]
AID - 10.1097/BPO.0000000000000897 [doi]
PST - ppublish
SO - J Pediatr Orthop. 2019 Apr;39(4):163-168. doi: 10.1097/BPO.0000000000000897.
PMID- 28734495
OWN - NLM
STAT- MEDLINE
DCOM- 20180626
LR - 20180626
IS - 0020-1383 (Linking)
VI - 48
IP - 10
DP - 2017 Oct
TI - Clinical experiences in the use of a gentamicin-coated titanium nail in tibia
fractures.
PG - 2235-2241
LID - S0020-1383(17)30427-8 [pii]
LID - 10.1016/j.injury.2017.07.008 [doi]
AB - Despite the improvement of surgical techniques surgical site infections
(SSIs) still
remain clinically challenging in high risk patients undergoing osteosynthesis
for
tibia fractures. The use of an antibiotic coated implant might reduce the
adhesion
of bacteria on the implant surface and could therefore reduce the rate of
implant-related infection or osteomyelitis. A gentamicin-coated tibia nail
was
evaluated in a prospective study. Four centers enrolled 100 patients (99
treated)
with fresh open or closed tibia fractures, or for non-union revision surgery
and
followed them for 18 months. Data collected included infection events,
radiographs,
SF-12, EQ-5D, Iowa Ankle score, and the WOMAC questionnaire. Sixty-eight of
the 99
treated patients suffered from a fresh fracture, while in 31 patients, the
intramedullary nail was implanted for revision purposes, including non-unions
due to
infection. Fifteen (22%) of the fresh fractures were GA Type III. The follow-
up rate
was 87% and 82% at 12 months and 18 months, respectively. Deep surgical site
infections occurred in 3 fresh fractures and two in revision surgeries. We
did not
observe any local or systemic toxic effects related to gentamicin during this
study.
The use of the antibiotic coated nail is an option in patients with a high
infection
risk, like open factures or infected non unions, in the prevention of the
onset of
an implant-related infection or osteomyelitis.
FAU - Schmidmaier, G
AU - Schmidmaier G
AD - Trauma and Reconstructive Surgery HTRG, Center of Orthopaedics, Traumatology
and
Spinal Cord Injury - University Clinic of Heidelberg, Schlierbacher
Landstraße 200a,
69118 Heidelberg, Germany. Electronic address:
gerhard.schmidmaier@med.uni-heidelberg.de.
FAU - Kerstan, M
AU - Kerstan M
AD - Clinical Research, DePuy Synthes, Zuchwil, Switzerland.
FAU - Schwabe, P
AU - Schwabe P
AD - Trauma- and Reconstructive Surgery, CMSC, Charité University Medicine,
Germany.
FAU - Südkamp, N
AU - Südkamp N
AD - Clinic for Orthopaedics and Trauma Surgery, Department of Surgery, University
Clinic
of Freiburg, Germany.
FAU - Raschke, M
AU - Raschke M
AD - Clinic for Trauma-, Hand-, and Reconstructive Surgery, University Clinic
Münster,
Germany.
LA - eng
PT - Multicenter Study
DEP - 20170710
PL - Netherlands
TA - Injury
JT - Injury
JID - 0226040
RN - 0 (Gentamicins)
SB - IM
MH - Adult
MH - *Anti-Bacterial Agents/administration & dosage
MH - *Bone Nails
MH - Female
MH - Fracture Fixation, Intramedullary/*methods
MH - *Gentamicins
MH - Humans
MH - Male
MH - Middle Aged
MH - Osteomyelitis/etiology/microbiology/*prevention & control
MH - Surgical Wound Infection/microbiology/*prevention & control
MH - Tibial Fractures/complications/microbiology/*surgery
MH - Titanium
OTO - NOTNLM
OT - Closed tibial fracture
OT - ETN Protect
OT - Gentamicin
OT - Implant coating
OT - Infection prophylaxis
OT - Open tibial fracture
OT - Tibial nail
EDAT- 2017/07/25 06:00
MHDA- 2018/06/27 06:00
CRDT- 2017/07/24 06:00
PHST- 2017/01/20 00:00 [received]
PHST- 2017/07/03 00:00 [revised]
PHST- 2017/07/09 00:00 [accepted]
PHST- 2017/07/25 06:00 [pubmed]
PHST- 2018/06/27 06:00 [medline]
PHST- 2017/07/24 06:00 [entrez]
AID - S0020-1383(17)30427-8 [pii]
AID - 10.1016/j.injury.2017.07.008 [doi]
PST - ppublish
SO - Injury. 2017 Oct;48(10):2235-2241. doi: 10.1016/j.injury.2017.07.008. Epub
2017 Jul
10.
PMID- 29772391
OWN - NLM
STAT- MEDLINE
DCOM- 20180720
LR - 20181202
IS - 1011-1344 (Linking)
VI - 183
DP - 2018 Jun
TI - Development of ionic liquid assisted-synthesized nano-silver combined with
vascular
endothelial growth factor as wound healing in the care of femoral fracture in
the
children after surgery.
PG - 385-390
LID - S1011-1344(18)30100-3 [pii]
LID - 10.1016/j.jphotobiol.2018.03.003 [doi]
AB - This investigation aimed to develop the silver (Ag) nanoparticles
incorporated
vascular endothelial growth factor (VEGF) for the improvement wound healing
and
reduce Aseptic necrosis in treatment of femoral fracture healing. The
spherical
shaped Ag nanoparticles with improved morphology have been effectively
synthesized
via microwave assisted method using ionic liquids 1-dodecyl-3-
methylimidazolium
chloride. The morphological structure and crystalline properties of Ag
nanoparticles
are analyzed by using UV, XRD and TEM-EDX analytical methods. The average
grain size
of the Ag nanoparticles is 20 nm, which was observed by defining the width of
the
(111) Bragg reflection with the Debye-Scherer formula and TEM results. The
biological analyses confirmed that the Ag nanoparticles with VEGF molecules
are
promoted the cell adhesion and proliferation of Human mesenchymal stem cells
(MSCs)
cells. Ag NPs at appropriate concentrations have favorable biocompatibility
to
encourage cell activation properties like cell proliferation, cytokines
release and
chemotaxis. In the present study, our experimental results indicated that Ag
NPs
incorporated VEGF material are highly favorable to fracture healing and
mainly as
blood vessel repair. The surface morphology improved synthetic Ag NPs using
ionic
liquids has shown advantageous for cell activity and also improve the
materials
performances with VEGF for the regeneration of femoral fractures.
CI - Copyright © 2018. Published by Elsevier B.V.
FAU - Li, Xingli
AU - Li X
AD - NO.1 Department of Pediatric Internal Medicine, Linyi Central Hospital, No.
17,
Jiankang Road, Yishui, Shandong Province 276400, PR China.
FAU - Liu, Maohua
AU - Liu M
17,
FAU - Cheng, Hongxia
AU - Cheng H
AD - Operation room, Yantai Yuhuangding Hospital, No. 20 Yuhuangding East road,
Shangdong
Province 264000, PR China.
FAU - Wang, Qun
AU - Wang Q
17,
Jiankang Road, Yishui, Shandong Province 276400, PR China. Electronic
address:
qwang008@rediffmail.com.
FAU - Miao, Chuanna
AU - Miao C
17,
FAU - Ju, Shumei
AU - Ju S
17,
FAU - Liu, Fang
AU - Liu F
17,
LA - eng
DEP - 20180306
PL - Switzerland
TA - J Photochem Photobiol B
JT - Journal of photochemistry and photobiology. B, Biology
JID - 8804966
RN - 0 (Cytokines)
RN - 0 (Ionic Liquids)
RN - 3M4G523W1G (Silver)
SB - IM
MH - Child
MH - Cytokines/metabolism
MH - Drug Carriers/*chemistry
MH - Femoral Fractures/pathology/surgery
MH - Femur Head/pathology
MH - Humans
MH - Ionic Liquids/*chemistry
MH - Mesenchymal Stem Cells/cytology/drug effects/metabolism
MH - Metal Nanoparticles/*chemistry
MH - Particle Size
MH - Silver/*chemistry
MH - Vascular Endothelial Growth Factor A/*chemistry/pharmacology/therapeutic use
OTO - NOTNLM
OT - Children after surgery
OT - Femoral fracture
OT - Ionic liquid
OT - Nanoparticles
OT - Silver
OT - VEGF
EDAT- 2018/05/18 06:00
MHDA- 2018/07/22 06:00
CRDT- 2018/05/18 06:00
PHST- 2018/01/29 00:00 [received]
PHST- 2018/02/25 00:00 [revised]
PHST- 2018/03/05 00:00 [accepted]
PHST- 2018/05/18 06:00 [pubmed]
PHST- 2018/07/22 06:00 [medline]
PHST- 2018/05/18 06:00 [entrez]
AID - S1011-1344(18)30100-3 [pii]
AID - 10.1016/j.jphotobiol.2018.03.003 [doi]
PST - ppublish
SO - J Photochem Photobiol B. 2018 Jun;183:385-390. doi:
10.1016/j.jphotobiol.2018.03.003. Epub 2018 Mar 6.
PMID- 21344438
OWN - NLM
STAT- MEDLINE
DCOM- 20110414
LR - 20161125
VI - 121
IP - 3
DP - 2011 Mar
TI - Traumatic dislocation of the incudostapedial joint repaired with fibrin
tissue
adhesive.
PG - 577-9
LID - 10.1002/lary.21427 [doi]
AB - We present a case of traumatic dislocation of the incudostapedial joint (ISJ)
and a
simple method for controlled application of the glue using commercial fibrin
tissue
adhesive. A 26-year-old female presented to our ENT clinic for hearing
impairment to
her left ear 2 months after a head trauma due to a motorcycle accident. The
audiogram revealed a 40- to 50-dB HL conductive hearing loss with a notch
configuration in bone conduction curve on the left ear. Computed tomography
of the
left temporal bone revealed a longitudinal fracture line. An exploratory
tympanotomy
was performed under general anesthesia. The ISJ was found dislocated while
the incus
was trapped by the edges of the bony lateral attic wall fracture. A small
bony edge
that impeded incus movement was removed and a small amount of the glue was
precisely
applied to the lenticular process of the incus with an angled incision knife.
The
long process of the incus was firmly pressed over the stapes for 30 seconds
with a
90° hook and 60 seconds after the application of the glue the ISJ was
repaired. One
year after our patient achieved full airbone gap (ABG) closure (ABG, ≤10 dB
HL),
while she demonstrated overclosure in frequencies 2 and 4 kHz. Fibrin tissue
glue
allowed safe, rapid, and accurate repair of the ISJ and resulted in an
anatomically
normal articulation as the mass and shape of the ossicles was preserved.
Moreover,
our patient achieved full ABG closure.
CI - Copyright © 2011 The American Laryngological, Rhinological, and Otological
Society,
Inc.
FAU - Nikolaidis, Vasilios
AU - Nikolaidis V
AD - From the Second University ENT Clinic of Aristotle University of
Thessaloniki,
Papageorgiou G. Hospital, Greece. nikldis@ath.forthnet.gr
LA - eng
PT - Case Reports
DEP - 20110113
PL - United States
TA - Laryngoscope
JT - The Laryngoscope
JID - 8607378
SB - IM
MH - Adult
MH - Female
MH - Fibrin Tissue Adhesive/*administration & dosage
MH - Head Injuries, Closed/*complications/diagnostic imaging
MH - Hearing Loss, Conductive/diagnostic imaging/*surgery
MH - Humans
MH - Incus/diagnostic imaging/*injuries/*surgery
MH - Joint Dislocations/diagnostic imaging/*surgery
MH - Radiography
MH - Skull Fractures/complications/diagnostic imaging
MH - Stapes/diagnostic imaging/*injuries
MH - Temporal Bone/diagnostic imaging/injuries
MH - Tissue Adhesives/*administration & dosage
EDAT- 2011/02/24 06:00
MHDA- 2011/04/16 06:00
CRDT- 2011/02/24 06:00
PHST- 2010/09/14 00:00 [received]
PHST- 2010/10/27 00:00 [accepted]
PHST- 2011/02/24 06:00 [entrez]
PHST- 2011/02/24 06:00 [pubmed]
PHST- 2011/04/16 06:00 [medline]
AID - 10.1002/lary.21427 [doi]
PST - ppublish
SO - Laryngoscope. 2011 Mar;121(3):577-9. doi: 10.1002/lary.21427. Epub 2011 Jan
13.
PMID- 22539160
OWN - NLM
STAT- MEDLINE
DCOM- 20130404
LR - 20181202
IS - 0341-2695 (Print)
IS - 0341-2695 (Linking)
VI - 36
IP - 10
DP - 2012 Oct
TI - Improvement of intertrochanteric bone quality in osteoporotic female rats
after
injection of polylactic acid-polyglycolic acid copolymer/collagen type I
microspheres combined with bone mesenchymal stem cells.
PG - 2163-71
LID - 10.1007/s00264-012-1543-4 [doi]
AB - PURPOSE: Osteoporosis mainly involves cancellous bone, and the spine and hip,
with
their relatively high cancellous bone to cortical bone ratio, are severely
affected.
Studies of bone mesenchymal stem cells (BMSCs) from osteoporotic patients and
animal
models have revealed that osteoporosis is often associated with reduction of
BMSCs'
proliferation and osteogenic differentiation. Our aim was to test whether
polylactic
acid-polyglycolic acid copolymer(PLGA)/collagen type I(CoI) microspheres
combined
with BMSCs could be used as injectable scaffolds to improve bone quality in
osteoporotic female rats. METHODS: PLGA microspheres were coated with CoI.
BMSCs of
the third passage and were cultured with PLGA/CoI microspheres for seven
days. Forty
three-month-old female non-pregnant SD rats were ovariectomized to establish
osteoporotic animal models. Three months after being ovariectomized, the
osteoporotic rats were randomly divided into five groups: SHAM group, PBS
group,
cell group, microsphere (MS) group, and cell+MS group. Varying materials were
injected into the intertrochanters of each group's rats. Twenty rats were
sacrificed
at one month and three months post-op, respectively. The femora were
harvested in
order to measure the intertrochanteric bone mineral density (BMD) with DEXA
and
trabecular thickness (Tb.Th), percentage of trabecular area (%Tb.Ar), bone
volume
fraction (BV/TV) and trabecular spacing (Tb.Sp) with Micro CT. One-way ANOVA
and
Kruskal-Wallis tests were used. RESULTS: BMSCs seeded on PLGA/CoI
microspheres had a
nice adhesion and proliferation. At one month post-op, the BMD (0.33 ± 0.01
g/cm(2)), Tb.Th (459.65 ± 28.31 μm), %Tb.Ar (9.61 ± 0.29 %) and Tb.Sp
(2645.81 ±
94.91 μm) of the cell+ MS group were better than those of the SHAM group and
the
cell group. At three months post-op, the BMD (0.32 ± 0.01 g/cm(2)), Tb.Th
(372.81 ±
38.45 μm), %Tb.Ar (6.65 ± 0.25 %), BV/TV (6.62 ± 0.25 %) and Tb.Sp (1559.03 ±
57.06
μm) of the cell + MS group were also better than those of the SHAM group and
the
cell group. CONCLUSION: The PLGA/CoI microspheres combined with BMSCs can
repair
bone defects more quickly. This means that PLGA/CoI microspheres combined
with BMSCs
can promote trabecular reconstruction and improve bone quality in
osteoporotic rats.
This scaffold can provide a promising minimally invasive surgical tool for
enhancement of bone fracture healing or prevention of fracture occurrence
which will
in turn minimize complications endemic to patients with osteoporosis.
FAU - Yu, Zhengrong
AU - Yu Z
AD - Department of Orthopedics, Peking University 1st Hospital, Beijing, China.
FAU - Zhu, Tianyue
AU - Zhu T
FAU - Li, Chunde
AU - Li C
FAU - Shi, Xudong
AU - Shi X
FAU - Liu, Xianyi
AU - Liu X
FAU - Yang, Xin
AU - Yang X
FAU - Sun, Haolin
AU - Sun H
LA - eng
DEP - 20120427
TA - Int Orthop
JT - International orthopaedics
JID - 7705431
SB - IM
MH - Animals
MH - Bone Density/*drug effects/physiology
MH - Coated Materials, Biocompatible
MH - Collagen Type I/chemistry/*pharmacology
MH - Female
MH - Femur/diagnostic imaging/drug effects/metabolism
MH - Lactic Acid/*administration & dosage
MH - *Mesenchymal Stem Cell Transplantation
MH - Mesenchymal Stem Cells/*cytology
MH - Microspheres
MH - Osteoporosis/metabolism/pathology/*therapy
MH - Ovariectomy
MH - Polyglycolic Acid/*administration & dosage
MH - Radiography
MH - Rats
PMC - PMC3460074
EDAT- 2012/04/28 06:00
MHDA- 2013/04/05 06:00
CRDT- 2012/04/28 06:00
PHST- 2012/03/07 00:00 [received]
PHST- 2012/04/02 00:00 [accepted]
PHST- 2012/04/28 06:00 [entrez]
PHST- 2012/04/28 06:00 [pubmed]
PHST- 2013/04/05 06:00 [medline]
AID - 1543 [pii]
AID - 10.1007/s00264-012-1543-4 [doi]
PST - ppublish
SO - Int Orthop. 2012 Oct;36(10):2163-71. doi: 10.1007/s00264-012-1543-4. Epub
2012 Apr
27.
PMID- 22934667
OWN - NLM
STAT- MEDLINE
DCOM- 20130711
LR - 20181202
IS - 1937-3341 (Print)
IS - 1937-3341 (Linking)
VI - 19
IP - 3-4
DP - 2013 Feb
TI - Fabrication of blended polycaprolactone/poly(lactic-co-glycolic acid)/β-
tricalcium
phosphate thin membrane using solid freeform fabrication technology for
guided bone
regeneration.
PG - 317-28
LID - 10.1089/ten.TEA.2011.0730 [doi]
AB - This study developed a bioabsorbable-guided bone regeneration membrane made
of
blended polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and
beta-tricalcium phosphate (β-TCP) using solid freeform fabrication (SFF)
technology.
The chemical and physical properties of the membrane were evaluated using
field
emission scanning electron microscopy, energy dispersive spectroscopy, and a
tensile
test. In vitro cell activity assays revealed that the adhesion,
proliferation, and
osteogenic differentiation of seeded adipose-derived stem cells (ADSCs) were
significantly promoted by the PCL/PLGA/β-TCP membranes compared with PCL/PLGA
membranes. When the PCL/PLGA and PCL/PLGA/β-TCP membranes were implanted on

rabbit
calvaria bone defects without ADSCs, microcomputed tomography and
histological
analyses confirmed that the SFF-based PCL/PLGA/β-TCP membranes greatly
increased
bone formation without the need for bone substitute materials. Moreover,
tight
integration, which helps to prevent exposure of the membrane, between both
membranes
and the soft tissues was clearly observed histologically. The SFF-based
PCL/PLGA and
PCL/PLGA/β-TCP membranes retained their mechanical stability for up to 8
weeks
without significant collapse. Furthermore, PCL/PLGA/β-TCP underwent adequate
degradation without a significant immune response at 8 weeks.
FAU - Shim, Jin-Hyung
AU - Shim JH
AD - Department of Mechanical Engineering, POSTECH, Gyeongbuk, Korea.
FAU - Huh, Jung-Bo
AU - Huh JB
FAU - Park, Ju Young
AU - Park JY
FAU - Jeon, Young-Chan
AU - Jeon YC
FAU - Kang, Seong Soo
AU - Kang SS
FAU - Kim, Jong Young
AU - Kim JY
FAU - Rhie, Jong-Won
AU - Rhie JW
FAU - Cho, Dong-Woo
AU - Cho DW
LA - eng
DEP - 20121004
TA - Tissue Eng Part A
JT - Tissue engineering. Part A
JID - 101466659
RN - 0 (Membranes, Artificial)
RN - 0 (Polyesters)
RN - 0 (beta-tricalcium phosphate)
SB - IM
MH - Animals
MH - Biocompatible Materials/chemical synthesis
MH - Guided Tissue Regeneration/*instrumentation
MH - Lactic Acid/*chemistry
MH - *Membranes, Artificial
MH - Polyesters/*chemistry
MH - Polyglycolic Acid/*chemistry
MH - Rabbits
MH - Skull Fractures/diagnosis/*surgery
PMC - PMC3542872
EDAT- 2012/09/01 06:00
MHDA- 2013/07/13 06:00
CRDT- 2012/09/01 06:00
PHST- 2012/09/01 06:00 [entrez]
PHST- 2012/09/01 06:00 [pubmed]
PHST- 2013/07/13 06:00 [medline]
AID - 10.1089/ten.tea.2011.0730 [pii]
AID - 10.1089/ten.TEA.2011.0730 [doi]
PST - ppublish
SO - Tissue Eng Part A. 2013 Feb;19(3-4):317-28. doi: 10.1089/ten.TEA.2011.0730.
Epub
2012 Oct 4.
PMID- 24365600
OWN - NLM
STAT- MEDLINE
DCOM- 20150105
LR - 20181202
IS - 1873-5061 (Linking)
VI - 12
IP - 2
DP - 2014 Mar
TI - Knockdown of SVCT2 impairs in-vitro cell attachment, migration and wound
healing in
bone marrow stromal cells.
PG - 354-63
LID - S1873-5061(13)00164-5 [pii]
LID - 10.1016/j.scr.2013.11.002 [doi]
AB - Bone marrow stromal cell (BMSC) adhesion and migration are fundamental to a
number
of pathophysiologic processes, including fracture and wound healing. Vitamin
C is
beneficial for bone formation, fracture repair and wound healing. However,
the role
of the vitamin C transporter in BMSC adhesion, migration and wound healing is
not
known. In this study, we knocked-down the sodium-dependent vitamin C
transporter,
SVCT2, the only known transporter of vitamin C in BMSCs, and performed cell
adhesion, migration, in-vitro scratch wound healing and F-actin re-
arrangement
studies. We also investigated the role of oxidative stress on the above
processes.
Our results demonstrate that both oxidative stress and down-regulation of
SVCT2
decreased cell attachment and spreading. A trans-well cell migration assay
showed
that vitamin C helped in BMSC migration and that knockdown of SVCT2 decreased
cell
migration. In the in-vitro scratch wound healing studies, we established that
oxidative stress dose-dependently impairs wound healing. Furthermore, the

supplementation of vitamin C significantly rescued the BMSCs from oxidative
stress
and increased wound closing. The knockdown of SVCT2 in BMSCs strikingly
decreased
wound healing, and supplementing with vitamin C failed to rescue cells
efficiently.
The knockdown of SVCT2 and induction of oxidative stress in cells produced an
alteration in cytoskeletal dynamics. Signaling studies showed that oxidative

stress
phosphorylated members of the MAP kinase family (p38) and that vitamin C
inhibited
their phosphorylation. Taken together, these results indicate that both the
SVCT2
transporter and oxidative stress play a vital role in BMSC attachment,
migration and
cytoskeletal re-arrangement. BMSC-based cell therapy and modulation of SVCT2
could
lead to a novel therapeutic approach that enhances bone remodeling, fracture
repair
and wound healing in chronic disease conditions.
CI - Published by Elsevier B.V.
FAU - Sangani, Rajnikumar
AU - Sangani R
AD - Department of Orthopaedic Surgery, Georgia Regents University, Augusta, GA
30912,
USA.
FAU - Pandya, Chirayu D
AU - Pandya CD
AD - Department of Psychiatry and Health Behavior, Georgia Regents University,
Augusta,
GA 30912, USA.
FAU - Bhattacharyya, Maryka H
AU - Bhattacharyya MH
30912,
USA.
FAU - Periyasamy-Thandavan, Sudharsan
AU - Periyasamy-Thandavan S
AD - Cellular Biology and Anatomy, Georgia Regents University, Augusta, GA 30912,
USA.
FAU - Chutkan, Norman
AU - Chutkan N
30912,
USA.
FAU - Markand, Shanu
AU - Markand S
USA.
FAU - Hill, William D
AU - Hill WD
USA;
Institute of Regenerative and Reparative Medicine, Georgia Regents
University,
Augusta, GA 30912, USA.
FAU - Hamrick, Mark
AU - Hamrick M
USA;
Institute of Regenerative and Reparative Medicine, Georgia Regents
University,
FAU - Isales, Carlos
AU - Isales C
30912,
USA; Institute of Regenerative and Reparative Medicine, Georgia Regents
University,
FAU - Fulzele, Sadanand
AU - Fulzele S
30912,
USA; Institute of Regenerative and Reparative Medicine, Georgia Regents
University,
Augusta, GA 30912, USA. Electronic address: sfulzele@gru.edu.
LA - eng
DEP - 20131109
PL - England
TA - Stem Cell Res
JT - Stem cell research
JID - 101316957
RN - 0 (Slc23a2 protein, mouse)
RN - 0 (Sodium-Coupled Vitamin C Transporters)
SB - IM
MH - Animals
MH - Bone Marrow Cells/*cytology/metabolism
MH - Cell Movement/physiology
MH - Gene Knockdown Techniques
MH - Mesenchymal Stem Cells/*cytology/metabolism
MH - Mice
MH - Oxidative Stress/physiology
MH - Sodium-Coupled Vitamin C Transporters/genetics/*metabolism
MH - Up-Regulation
MH - Wound Healing/*physiology
EDAT- 2013/12/25 06:00
MHDA- 2015/01/06 06:00
CRDT- 2013/12/25 06:00
PHST- 2013/05/28 00:00 [received]
PHST- 2013/10/01 00:00 [revised]
PHST- 2013/11/03 00:00 [accepted]
PHST- 2013/12/25 06:00 [entrez]
PHST- 2013/12/25 06:00 [pubmed]
PHST- 2015/01/06 06:00 [medline]
AID - S1873-5061(13)00164-5 [pii]
AID - 10.1016/j.scr.2013.11.002 [doi]
PST - ppublish
SO - Stem Cell Res. 2014 Mar;12(2):354-63. doi: 10.1016/j.scr.2013.11.002. Epub
2013 Nov
9.
PMID- 26018619
OWN - NLM
STAT- MEDLINE
DCOM- 20160408
LR - 20181113
VI - 10
DP - 2015 May 28
TI - In vivo animal study and clinical outcomes of autologous atelocollagen-
induced
chondrogenesis for osteochondral lesion treatment.
PG - 82
LID - 10.1186/s13018-015-0212-x [doi]
LID - 82
AB - BACKGROUND: Collagen acts as a scaffold for healing damaged cartilage. This
study
evaluated the results of an in vivo animal study and provides short-term
clinical
results on a mixture of atelocollagen and fibrin glue-enhanced microfracture
techniques in patients with osteochondral lesions (OCL) of the talus.
METHODS: This
paper contains animal in vivo data and clinical outcomes on the effectiveness
of
atelocollagen. An in vivo animal study was conducted with full-thickness
cartilage
defects created in the femoral condyle of 12 rabbits equally divided into 4
groups
evaluated at 2, 4, 8, and 12 weeks. Four chondral lesions were created
according to
one procedure on each rabbit with each lesion treated as follows: (1)
microfracture,
(2) microfracture and the lesion covered with atelocollagen, (3)
microfracture and
the lesion covered with mixture of atelocollagen and fibrin glue, and (4)
microfracture and the lesion covered with fibrin glue. In the clinical
evaluation,
17 patients were treated with a combination of microfracture and
atelocollagen
injection for symptomatic full-thickness OCL of the talus. They were
evaluated by
the American Orthopedic Foot and Ankle Society Ankle-Hindfoot Score (AOFAS),
Hannover Ankle Score System (HSS), visual analog scale (VAS), and magnetic
resonance
imaging (MRI) at baseline and at 12-months follow-up. Magnetic Resonance
Observation
of Cartilage Repair Tissue (MOCART) score of the post-op status was compared
with
the MOCART score and a modified Anderson's score of the pre-op status.
RESULTS: In
the animal study, subchondral bone and cartilage were generated completely in
groups
2 and 3 microscopically. Hyaline-like cartilage was found in the repair
tissue. In
the clinical evaluation, mean AOFAS improved from 62 to 88, mean HSS improved
from
62 to 87, and mean VAS score improved from 64 to 18, respectively (p <0.001).
Fifteen patients (89%) reported good or excellent satisfaction. We defined

the
improvement of most of the subchondral bone edema and bone cyst as well as a
chondral lesion by radiologic evaluation. CONCLUSIONS: Rapid regeneration of
cartilage was demonstrated in the in vivo animal study, and patients showed
significant clinical improvement. Atelocollagen-enhanced microfracture
enabled a
reasonable treatment of cartilage defects.
FAU - Kim, Jinsu
AU - Kim J
AD - Department of Foot and Ankle Surgery, Eulji General Hospital, College of
Medicine,
Eulji University, 14 Hangeulbiseok-Gil, Nowon-gu, Seoul, 139-711, South
Korea.
FAU - Cho, Hunki
AU - Cho H
Medicine,
Korea.
part4uos@naver.com.
FAU - Young, Kiwon
AU - Young K
Medicine,
Korea.
FAU - Park, Jaehyun
AU - Park J
Medicine,
Korea.
FAU - Lee, Junkeun
AU - Lee J
AD - RMS, SewonCellontech, Seongdong-gu, Seoul, South Korea.
FAU - Suh, Dongsam
AU - Suh D
AD - 8 F Wooyoung Techno Center, 273-15, Seongsu 2ga 3-dong, Seongdong-gu, Seoul,
South
Korea.
LA - eng
DEP - 20150528
JID - 101265112
RN - 0 (atelocollagen)
SB - IM
MH - Adult
MH - Animals
MH - Cartilage, Articular/*injuries/surgery
MH - Chondrogenesis/*drug effects
MH - Collagen/*therapeutic use
MH - Female
MH - Fractures, Bone/surgery/therapy
MH - Humans
MH - Male
MH - Rabbits
MH - Talus/*injuries/surgery
PMC - PMC4449973
EDAT- 2015/05/29 06:00
MHDA- 2016/04/09 06:00
CRDT- 2015/05/29 06:00
PHST- 2014/10/27 00:00 [received]
PHST- 2015/05/04 00:00 [accepted]
PHST- 2015/05/29 06:00 [entrez]
PHST- 2015/05/29 06:00 [pubmed]
PHST- 2016/04/09 06:00 [medline]
AID - 10.1186/s13018-015-0212-x [pii]
AID - 212 [pii]
AID - 10.1186/s13018-015-0212-x [doi]
PST - epublish
SO - J Orthop Surg Res. 2015 May 28;10:82. doi: 10.1186/s13018-015-0212-x.
PMID- 28155060
OWN - NLM
STAT- MEDLINE
DCOM- 20180404
LR - 20190112
IS - 1590-9921 (Print)
IS - 1590-9921 (Linking)
VI - 18
IP - 2
DP - 2017 Jun
TI - Fast-resorbable antibiotic-loaded hydrogel coating to reduce post-surgical
infection
after internal osteosynthesis: a multicenter randomized controlled trial.
PG - 159-169
LID - 10.1007/s10195-017-0442-2 [doi]
AB - BACKGROUND: Infection is one of the main reasons for failure of orthopedic
implants.
Antibacterial coatings may prevent bacterial adhesion and biofilm formation,
according to various preclinical studies. The aim of the present study is to
report
the first clinical trial on an antibiotic-loaded fast-resorbable hydrogel
coating
(Defensive Antibacterial Coating, DAC(®)) to prevent surgical site infection,
in
patients undergoing internal osteosynthesis for closed fractures. MATERIALS
AND
METHODS: In this multicenter randomized controlled prospective study, a total
of 256
patients in five European orthopedic centers who were scheduled to receive
osteosynthesis for a closed fracture, were randomly assigned to receive
antibiotic-loaded DAC or to a control group (without coating). Pre- and
postoperative assessment of laboratory tests, wound healing, clinical scores
and
X-rays were performed at fixed time intervals. RESULTS: Overall, 253 patients
were
available with a mean follow-up of 18.1 ± 4.5 months (range 12-30). On
average,
wound healing, clinical scores, laboratory tests and radiographic findings
did not
show any significant difference between the two groups. Six surgical site
infections
(4.6%) were observed in the control group compared to none in the treated
group
(P < 0.03). No local or systemic side-effects related to the DAC hydrogel
product
were observed and no detectable interference with bone healing was noted.
CONCLUSIONS: The use of a fast-resorbable antibiotic-loaded hydrogel implant
coating
provides a reduced rate of post-surgical site infections after internal
osteosynthesis for closed fractures, without any detectable adverse event or
side-effects. LEVEL OF EVIDENCE: 2.
FAU - Malizos, Kostantinos
AU - Malizos K
AD - Orthopaedic Surgery and Trauma, Medical School, University of Thessaly,
Larissa,
Greece.
FAU - Blauth, Michael
AU - Blauth M
AD - Department for Trauma Surgery, Medical University, Innsbruck, Austria.
FAU - Danita, Adrian
AU - Danita A
AD - Department for Trauma Surgery, Medical University, Innsbruck, Austria.
FAU - Capuano, Nicola
AU - Capuano N
AD - Department for Orthopaedics, San Luca Hospital, Vallo Della Lucania, Italy.
FAU - Mezzoprete, Riccardo
AU - Mezzoprete R
AD - Department for Orthopaedics, San Camillo de Lellis Hospital, Rieti, Italy.
FAU - Logoluso, Nicola
AU - Logoluso N
AD - Department of Reconstructive Surgery of Osteo-articular Infections CRIO Unit,
IRCCS
Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy.
FAU - Drago, Lorenzo
AU - Drago L
AD - Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic
Institute, Milan, Italy.

AD - Laboratory of Medical Technical Sciences, Department of Biochemical Sciences
for
Health, University of Milano, Milan, Italy.
FAU - Romanò, Carlo Luca
AU - Romanò CL
AD - Department of Reconstructive Surgery of Osteo-articular Infections CRIO Unit,
IRCCS
Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy.
carlo.romano@grupposandonato.it.
LA - eng
PT - Randomized Controlled Trial
DEP - 20170202
TA - J Orthop Traumatol
JT - Journal of orthopaedics and traumatology : official journal of the Italian
Society
of Orthopaedics and Traumatology
JID - 101090931
RN - 0 (Drug Combinations)
RN - 0 (Hydrogels)
SB - IM
MH - Adult
MH - Aged
MH - Anti-Bacterial Agents/*pharmacology
MH - Drug Combinations
MH - Europe/epidemiology
MH - Female
MH - Fracture Fixation, Internal/*instrumentation
MH - Fractures, Closed/*surgery
MH - Humans
MH - Hydrogels/*pharmacology
MH - Incidence
MH - Male
MH - Middle Aged
MH - Surgical Wound Infection/epidemiology/*prevention & control
MH - Time Factors
MH - Young Adult
PMC - PMC5429256
OTO - NOTNLM
OT - Coating
OT - DAC
OT - Hydrogel
OT - Infection
OT - Osteosynthesis
OT - Prevention
COIS- CONFLICT OF INTEREST: The authors declare that they have no conflict of
interest.
PATIENT CONSENT: Informed consent was obtained from all individual
participants
included in the study. ETHICAL APPROVAL: All patients gave the informed
consent
prior being included into the study. All procedures involving human
participants
were in accordance with the 1964 Helsinki declaration and its later
amendments. The
study was approved by the responsible Research Ethics Committee or
Institutional
Review Board. FUNDING: The study was performed within the 7th European
Framework
Programme (project #277988) and funded by the European Commission and the
participating partners (clinical institutions and the following private
companies:
Novagenit SRL, Mezzolombardo, Italy, acting as project leader; AdlerOrtho
SRL,
Bologna, Italy; Arcos SARL, Brignoles, France; Belgafix SPRL, Drogenbos,
Belgium).
EDAT- 2017/02/06 06:00
MHDA- 2018/04/05 06:00
CRDT- 2017/02/04 06:00
PHST- 2016/07/19 00:00 [received]
PHST- 2016/12/31 00:00 [accepted]
PHST- 2017/02/06 06:00 [pubmed]
PHST- 2018/04/05 06:00 [medline]
PHST- 2017/02/04 06:00 [entrez]
AID - 10.1007/s10195-017-0442-2 [pii]
AID - 442 [pii]
AID - 10.1007/s10195-017-0442-2 [doi]
PST - ppublish
SO - J Orthop Traumatol. 2017 Jun;18(2):159-169. doi: 10.1007/s10195-017-0442-2.
Epub
2017 Feb 2.
PMID- 28415416
OWN - NLM
STAT- MEDLINE
DCOM- 20170724
LR - 20170724
IS - 0928-4931 (Linking)
VI - 75
DP - 2017 Jun 1
TI - In vitro and in vivo corrosion, mechanical properties and biocompatibility
evaluation of MgF(2)-coated Mg-Zn-Zr alloy as cancellous screws.
PG - 1268-1280
LID - S0928-4931(16)31859-8 [pii]
LID - 10.1016/j.msec.2017.02.168 [doi]
AB - Magnesium (Mg) and its alloys as biodegradable materials have received much
attention in the orthopedics applications; however, the corrosion behavior of
these
metals in vivo remains challenging. In this work, a dense and nanoscale
magnesium
fluoride (MgF(2)) coating was deposited on the surface of Mg-Zn-Zr (MZZ)
alloy
cancellous screw. The MZZ cancellous screw with MgF(2) coating maintained an
integrated shape and high yield tensile stress after 30days immersion in SBF,
comparing with the bare screw. Hydrogen releasing rate of the MZZ samples was
suppressed at a lower level at the initial stage, which is in favour of the

adhesion
of the cells. And in vivo experiments indicated that MgF(2)-coated MZZ screws
presented advantages in cytocompatibility, osteoconductivity and osteogenesis

of
cancellous bone in rabbits. Corrosion rate in vivo perfusion environment
increased
very slowly with time in long-term study, which was an opposite trend in
vitro
static immersion test. Moreover, maximum corrosion rate (CR(max)), a critical
calculation method of corrosion rate was introduced to predict fracture

regions of
the sample. The MZZ alloy with MgF(2) coating possesses a great potential for
clinical applications for internal fracture fixation repair.

FAU - Li, Zhen
AU - Li Z
AD - School of Materials Science and Engineering, Tianjin University of
Technology,
300384 Tianjin, China.
FAU - Shizhao, Sun
AU - Shizhao S
Technology,
FAU - Chen, Minfang
AU - Chen M
Technology,
300384 Tianjin, China. Electronic address: mfchentj@163.com.
FAU - Fahlman, Bradley Dean
AU - Fahlman BD
AD - Department of Chemistry & Biochemistry, , Central Michigan University, Mount
Pleasant, MI 48859, USA.
FAU - Debao Liu
AU - Debao Liu
Technology,
FAU - Bi, Hongwei
AU - Bi H
AD - Tianjin Meisheng Biological Technology Co., Ltd, 300384 Tianjin, China.
LA - eng
DEP - 20170306
PL - Netherlands
TA - Mater Sci Eng C Mater Biol Appl
JT - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN - 0 (Magnesium Compounds)
RN - 5N014C7IWU (magnesium fluoride)
RN - C6V6S92N3C (Zirconium)
RN - J41CSQ7QDS (Zinc)
RN - Q80VPU408O (Fluorides)
SB - IM
MH - Animals
MH - *Bone Screws
MH - Cell Line
MH - Corrosion
MH - Fluorides/*chemistry
MH - Magnesium/*chemistry
MH - Magnesium Compounds/*chemistry
MH - Mice
MH - Zinc/*chemistry
MH - Zirconium/*chemistry
OTO - NOTNLM
OT - Biocompatibility
OT - Corrosion rate
OT - Magnesium fluoride coating
OT - Mechanical test
OT - Mg-Zn-Zr alloy cancellous screws
OT - Micro-CT
EDAT- 2017/04/19 06:00
MHDA- 2017/07/25 06:00
CRDT- 2017/04/19 06:00
PHST- 2016/10/23 00:00 [received]
PHST- 2017/01/07 00:00 [revised]
PHST- 2017/02/28 00:00 [accepted]
PHST- 2017/04/19 06:00 [entrez]
PHST- 2017/04/19 06:00 [pubmed]
PHST- 2017/07/25 06:00 [medline]
AID - S0928-4931(16)31859-8 [pii]
AID - 10.1016/j.msec.2017.02.168 [doi]
PST - ppublish
SO - Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:1268-1280. doi:
10.1016/j.msec.2017.02.168. Epub 2017 Mar 6.
PMID- 25563014
OWN - NLM
STAT- MEDLINE
DCOM- 20150223
LR - 20161020
IS - 0869-6047 (Print)
IS - 0869-6047 (Linking)
IP - 7-8
DP - 2014
TI - [Fracture healing under intramedullary insertion of wires with hydroxyapatite
coating].
PG - 127-32
AB - AIM: To study morphological features of the bone formation process in
consolidation
of fractures of long tubular bones in conditions of intramedullary wires
insertion
with bioactive calcium-phosphate coating of hydroxyapatite. MATERIALS AND
METHODS:
In experimental study in dogs was simulated open comminuted tibia fracture
and
performed intramedullary insertion of wires with hydroxyapatite coating.
Using light
and electron microscopy, using X-ray electron microprobe microanalyses were
studied
bone regenerates in 14-360 days after surgery. RESULTS: It was found that
around
wires there is a formation of an area of active reparative bone formation and
angiogenesis, bone shaped case with the properties of the conductor and
inducer of
osteogenesis. Fracture consolidation is carried out in the early stages of
the
primary type without formation of cartilage and connective tissue in the bone
adhesion. CONCLUSION: Study results testify that intramedullary wires with

hydroxyapatite coating positively influence on the process and intensity of
reparative bone formation in fracture healing.
FAU - Ir'ianov, Iu M
AU - Ir'ianov IuM
FAU - Kir'ianov, N A
AU - Kir'ianov NA
FAU - Popkov, A V
AU - Popkov AV
LA - rus
PT - English Abstract
PL - Russia (Federation)
TA - Vestn Ross Akad Med Nauk
JT - Vestnik Rossiiskoi akademii meditsinskikh nauk
JID - 9215641
SB - IM
MH - Animals
MH - Coated Materials, Biocompatible/pharmacology
MH - Dogs
MH - Fracture Fixation, Intramedullary/*instrumentation/methods
MH - Fractures, Comminuted/surgery
MH - Materials Testing/methods
MH - Tibial Fractures/*surgery
EDAT- 2015/01/08 06:00
MHDA- 2015/02/24 06:00
CRDT- 2015/01/08 06:00
PHST- 2015/01/08 06:00 [entrez]
PHST- 2015/01/08 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
PST - ppublish
SO - Vestn Ross Akad Med Nauk. 2014;(7-8):127-32.
PMID- 30091422
OWN - NLM
STAT- MEDLINE
DCOM- 20190715
LR - 20190715
IS - 1748-6041 (Linking)
VI -13
IP -6
DP -2018 Aug 24
TI -3D printing of strontium-doped hydroxyapatite based composite scaffolds for
repairing critical-sized rabbit calvarial defects.
PG - 065004
LID - 10.1088/1748-605X/aad923 [doi]
AB - In this study, strontium substituted hydroxyapatite (Sr-HAP) was synthesized
using
collagen type I and citrate as bi-templates and the obtained nanoparticles
with high
similarity to natural bone minerals were made into composite scaffolds with
interconnected porous structure using a three-dimensional (3D) printing
technique. A
calcium deficient structure of HAP phase was caused by doping Sr which was
verified
by Fourier transform infrared, x-ray diffractometer, scanning electron
microscopy
and transmission electron microscopy. The Sr/(Sr + Ca) molar ratio in Sr-HAP
nanoparticles was 5.8% estimated by EDX. Furthermore, both 3D printed
scaffolds made
of Sr-HAP and HAP had uniform porous structure and porosity of about 60%.
Cell
culturing indicated that MC3T3-E1 cells could adhere on the surface of the
scaffolds
and the strontium substitution could enhance cell adhesion, proliferation and
alkaline phosphatase activity. The printed composite scaffolds were used to

repair
critical-sized rabbit calvarial defects with a diameter of 15 mm. The results
showed
that the Sr-HAP scaffolds had better osteogenic capability and stimulated
more new
bone formation within 12 weeks. It was suggested that these printed Sr-HAP
composite
scaffolds possessed high potential as candidates in the application of bone
augmentation and regeneration.
FAU - Luo, Yun
AU - Luo Y
AD - Advanced Biomaterials and Tissue Engineering Center, Huazhong University of
Science
and Technology, Wuhan 430074, People's Republic of China.
FAU - Chen, Shangsi
AU - Chen S
FAU - Shi, Yufei
AU - Shi Y
FAU - Ma, Jun
AU - Ma J
LA - eng
DEP - 20180824
PL - England
TA - Biomed Mater
JID - 101285195
RN - 0 (Powders)
RN - 0 (strontium hydroxyapatite)
SB - IM
MH - 3T3 Cells
MH - Animals
MH - Bone and Bones/*drug effects
MH - Calcification, Physiologic
MH - Cell Adhesion
MH - Collagen/chemistry
MH - Collagen Type I/*chemistry
MH - Hydroxyapatites/*chemistry
MH - Mice
MH - Osteogenesis
MH - Porosity
MH - Powders
MH - *Printing, Three-Dimensional
MH - Rabbits
MH - Strontium/*chemistry
EDAT- 2018/08/10 06:00
MHDA- 2019/07/16 06:00
CRDT- 2018/08/10 06:00
PHST- 2018/08/10 06:00 [pubmed]
PHST- 2019/07/16 06:00 [medline]
PHST- 2018/08/10 06:00 [entrez]
AID - 10.1088/1748-605X/aad923 [doi]
PST - epublish
SO - Biomed Mater. 2018 Aug 24;13(6):065004. doi: 10.1088/1748-605X/aad923.
PMID- 30528224
OWN - NLM
STAT- MEDLINE
DCOM- 20200124
LR - 20200124
IS - 0949-2658 (Linking)
VI - 24
IP - 4
DP - 2019 Jul
TI - Effects of extracorporeal fucosylation of CD44 on the homing ability of
rabbit bone
marrow mesenchymal stem cells.
PG - 725-730
LID - S0949-2658(18)30334-8 [pii]
LID - 10.1016/j.jos.2018.11.010 [doi]
AB - BACKGROUND: The aim of the study was to investigate the effects of
extracorporeal
fucosylation of CD44 on the homing ability of rabbit bone marrow mesenchymal
stem
cells (BMSCs). METHODS: The rabbit BMSCs were extracorporeal fucosylated
using
alpha-(1,3)-fucosyltransferase VI (FTVI), then the positive rate of sialyl-
LewisX
(sLe(X)) and the binding rate of E-selectin were detected by flow cytometry,
as well
as the fluid adhesion of rabbit BMSCs were detected by the parallel flow
chamber
adhesion test. Then BMSCs were constructed to stably express enhanced green
fluorescent protein (EGFP) and were injected intravenously into the model
rabbits
with tibial fractures. After 6 weeks of injection, the levels of stromal
cell-derived factor (SDF-1) and monocyte chemoattractant protein-1 (MCP-1) in
rabbit
serum and damaged bone tissues were detected. The positive rate of EGFP
expressions
was detected by immunohistochemistry staining. RESULTS: After fucosylation,
the
positive rate of sLe(X) and the binding rate of E-selectin were significantly
higher
than those in the no fucosylated group. The results of fluorescence
microscopy
showed that BMSCs with stable expression of EGFP were successfully
constructed. The
results of ELISA and Western Blot showed that the secretion of SDF-1 and MCP-
1 and
the expression of SDF-1 and MCP-1 protein in BMSCs treatment group processed
by
fucosylated were significantly higher than those in BMSCs treatment group
processed
by no fucosylated. The results of immunohistochemical staining showed that
the
positive rate of EGFP expression was also significantly increased, which
indicated
that the BMSCs at the injured bone tissues were significantly increased and
helpful
in the repair of bone injury. CONCLUSIONS: Extracorporeal fucosylation of
CD44
molecules can significantly enhance the homing ability of rabbit BMSCs, which
may be
achieved by SDF-1 and MCP-1 regulation.
CI - Copyright © 2018 The Japanese Orthopaedic Association. Published by Elsevier
B.V.
All rights reserved.
FAU - Xiao, Fei
AU - Xiao F
AD - Department of Orthopaedics, Wuhan Fourth Hospital, Puai Hospital, Tongji
Medical
College, Huazhong University of Science and Technology, Wuhan, Hubei, 430033,
China;
Department of Orthopaedic Surgery, Zhongnan Hospital, Wuhan University,
Wuhan,
Hubei, 430071, China.
FAU - Chen, Liaobin
AU - Chen L
AD - Department of Orthopaedic Surgery, Zhongnan Hospital, Wuhan University,
Wuhan,
Hubei, 430071, China. Electronic address: jia19811001@whu.edu.cn.
LA - eng
DEP - 20181206
PL - Japan
TA - J Orthop Sci
JT - Journal of orthopaedic science : official journal of the Japanese Orthopaedic
Association
JID - 9604934
RN - 0 (Chemokine CCL2)
RN - 0 (Chemokine CXCL12)
RN - 0 (Hyaluronan Receptors)
RN - EC 2.4.1.- (Fucosyltransferases)
SB - IM
MH - Animals
MH - Cell Culture Techniques
MH - Chemokine CCL2/metabolism
MH - Chemokine CXCL12/metabolism
MH - Fucosyltransferases/*pharmacology
MH - Hyaluronan Receptors/*metabolism
MH - Mesenchymal Stem Cells/*drug effects
MH - Rabbits
MH - Tibial Fractures/metabolism/pathology/*therapy
EDAT- 2018/12/12 06:00
MHDA- 2020/01/25 06:00
CRDT- 2018/12/12 06:00
PHST- 2018/07/17 00:00 [received]
PHST- 2018/10/23 00:00 [revised]
PHST- 2018/11/16 00:00 [accepted]
PHST- 2018/12/12 06:00 [pubmed]
PHST- 2020/01/25 06:00 [medline]
PHST- 2018/12/12 06:00 [entrez]
AID - S0949-2658(18)30334-8 [pii]
AID - 10.1016/j.jos.2018.11.010 [doi]
PST - ppublish
SO - J Orthop Sci. 2019 Jul;24(4):725-730. doi: 10.1016/j.jos.2018.11.010. Epub
2018 Dec
6.
PMID- 29985868
OWN - NLM
STAT- MEDLINE
DCOM- 20181109
LR - 20181109
IS - 0271-6798 (Linking)
VI - 38
IP - 9
DP - 2018 Oct
TI - Range of Motion Improvement Following Surgical Management of Knee
Arthrofibrosis in
Children and Adolescents.
PG - e495-e500
LID - 10.1097/BPO.0000000000001227 [doi]
AB - BACKGROUND: Arthrofibrosis of the knee is well-described in adults as a
potentially
debilitating postoperative complication following anterior cruciate ligament
reconstruction, total knee arthroplasty, or fracture fixation. Knee
arthrofibrosis
in children and adolescents, however, has received little attention. The
primary
purpose of this study was to report improvements in range of motion (ROM)
following
lysis of adhesions and manipulation under anesthesia (LOA/MUA) in children
and
adolescents with knee arthrofibrosis, and, secondarily, to evaluate for any
effect
of preoperative dynamic splinting on ROM outcomes. METHODS: Ninety patients
aged 18
years and below (mean, 14.4±3.5) and 31% male who underwent LOA/MUA at an
urban
tertiary care hospital following prior knee surgery were evaluated.
Demographic,
clinical, ROM, and revision data were compiled. Primary outcome was absolute
ROM.
Secondarily, ROM was analyzed as a categorical variable with "Full ROM"
defined to
be -5 to 130 degrees or better, "functional" ROM was defined as unable to
obtain -5
to 130 degrees but not requiring revision, and "failure" defined as resulting
in
revision arthrofibrosis surgery. t tests and χ analyses were used to compare
ROM and
count variables between dynamic splinting subgroups. RESULTS: Mean time from
index
surgery to LOA/MUA was 6.0±4.4 months, and follow-up was 42±56 months. Index
procedures included anterior cruciate ligament reconstruction (N=33), tibial
spine
arthroscopic reduction and internal fixation (N=18), fracture fixation
(N=17), soft
tissue repair (N=17), and multiligament reconstruction (N=5). In total, 68
subjects
(76%) had any flexion loss, 57 subjects (63%) had any extension loss, and 40
subjects (44%) had both flexion and extension loss.Fifty-six subjects (62%)
had full
ROM at final follow-up, 25 subjects (28%) had functional ROM, and 9 subjects
(10%)
required revision. No demographic, clinical, or surgical variable was
predictive of
treatment failure. Patients who underwent dynamic splinting preoperatively
(N=46;
51%) had greater preoperative flexion (99±16 vs.77±34 degrees; P=0.001), but
no
difference in flexion at final follow-up (121±20 vs.128±11 degrees; P=0.08).
Failure
was not associated with time from index procedure to LOA/MUA, and the
proportion who
regained full ROM postoperatively was equivalent between those who had
dynamic
splinting and those who did not (65% vs. 59%; P=0.70). CONCLUSIONS: LOA/MUA
for
children with arthrofibrotic knees results in significant improvements in ROM
with
90% revision-free success. Preoperative dynamic or static progressive
splinting
improves preoperative flexion but does not affect postoperative range of
motion or
failure rate. LEVEL OF EVIDENCE: Level II.
FAU - Fabricant, Peter D
AU - Fabricant PD
AD - Division of Pediatric Orthopaedic Surgery, Hospital for Special Surgery, New
York,
NY.
FAU - Tepolt, Frances A
AU - Tepolt FA
AD - Department of Orthopedic Surgery, Division of Sports Medicine, Boston
Children's
Hospital.
FAU - Kocher, Mininder S
AU - Kocher MS
AD - Department of Orthopedic Surgery, Division of Sports Medicine, Boston
Children's
Hospital.
AD - Department of Orthopedic Surgery, Harvard Medical School, Boston, MA.
LA - eng
PL - United States
JID - 8109053
SB - IM
CIN - J Pediatr Orthop. 2019 Nov/Dec;39(10):e797-e798. PMID: 30562276
CIN - J Pediatr Orthop. 2019 Nov/Dec;39(10):e796-e797. PMID: 31600175
MH - Adolescent
MH - Anterior Cruciate Ligament Reconstruction/*adverse effects
MH - Child
MH - Female
MH - Humans
MH - Joint Diseases/etiology/rehabilitation/*surgery
MH - Knee Joint/physiopathology/*surgery
MH - Male
MH - Postoperative Complications/etiology/*surgery
MH - *Range of Motion, Articular
MH - Reoperation
MH - *Splints
MH - Tibia/surgery
EDAT- 2018/07/10 06:00
MHDA- 2018/11/10 06:00
CRDT- 2018/07/10 06:00
PHST- 2018/07/10 06:00 [pubmed]
PHST- 2018/11/10 06:00 [medline]
PHST- 2018/07/10 06:00 [entrez]
AID - 10.1097/BPO.0000000000001227 [doi]
PST - ppublish
SO - J Pediatr Orthop. 2018 Oct;38(9):e495-e500. doi:
10.1097/BPO.0000000000001227.
PMID- 26334897
OWN - NLM
STAT- MEDLINE
DCOM- 20160211
LR - 20210109
IS - 0025-7974 (Print)
IS - 0025-7974 (Linking)
VI - 94
IP - 35
DP - 2015 Sep
TI - Flexor Tendon Entrapment at the Malunited Base Fracture of the Proximal
Phalanx of
the Finger in Child: A Case Report.
PG - e1408
LID - 10.1097/MD.0000000000001408 [doi]
LID - e1408
AB - The proximal phalangeal base is the most commonly fractured hand bone in
children.
Such fractures are rarely reported as irreducible due to flexor tendon
entrapment.
Here, we describe a patient who sustained a malunited fracture on the right
fifth
finger proximal phalanx with flexor tendon entrapment after treatment with
closed
reduction with K-wires fixation.A 13-year-old patient came to the clinic
following a
bicycle accident 6 weeks ago. He presented with flexion limitation in his
small
finger on the right hand. During physical examination, the patient felt no
pain, and
the neurovascular structures were intact. However range of motion (ROM) in
his small
finger was not normal. Plain radiographs displayed a Salter-Harris type II
fracture
of the small finger proximal phalanx base and volar angulation with callus
formation. During the operation, it was established that the flexor digitorum
superficialis (FDS) around the fracture had a severe adhesion, whereas the
flexor
digitorum profundus (FDP) was entrapped between the volarly displaced
metaphyses and
the epiphyses and united with the bone. We removed the volarly displaced
metaphyses
and freed FDP and repaired the A2 pulley. The bone was anatomically fixed
with
K-wires. In the treatment after the operation, on the 2nd day, the patient
was
permitted the DIP joint motion by wearing a dynamic splint.At the 12-months
follow-up, the patient had regained full ROM with no discomfort and the
proximal
phalanx growth plate of the small finger closed naturally with normal
alignment.When
treating a proximal phalangeal base fracture in children, the possibility of
flexor
tendon entrapment should be considered and should be carefully dealt with in
its
treatment.
FAU - Lee, Young-Keun
AU - Lee YK
AD - From the Department of Orthopedic Surgery, Chonbuk National University
Hospital,
Jeonju (Y-K L, M L); Graduate Scool of MOT/ Sogang Institute of Advanced
Technology,
Sogang University, Seoul, Korea.
FAU - Park, Soojin
AU - Park S
FAU - Lee, Malrey
AU - Lee M
LA - eng
PT - Case Reports
TA - Medicine (Baltimore)
JT - Medicine
JID - 2985248R
SB - AIM
SB - IM
EIN - Medicine (Baltimore). 2015 Sep;94(38):1
MH - Adolescent
MH - Bone Wires
MH - Finger Joint/physiology
MH - Finger Phalanges/*injuries
MH - Fractures, Bone/*complications/diagnostic imaging/surgery
MH - Fractures, Malunited/*complications/diagnostic imaging/surgery
MH - Humans
MH - Male
MH - Radiography
MH - Range of Motion, Articular/physiology
MH - Trigger Finger Disorder/*diagnosis/diagnostic imaging/physiopathology
PMC - PMC4616507
COIS- All named authors hereby declare that they have no conflicts of interest to
disclose.
EDAT- 2015/09/04 06:00
MHDA- 2016/02/13 06:00
CRDT- 2015/09/04 06:00
PHST- 2015/09/04 06:00 [entrez]
PHST- 2015/09/04 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
AID - 00005792-201509010-00017 [pii]
AID - 10.1097/MD.0000000000001408 [doi]
PST - ppublish
SO - Medicine (Baltimore). 2015 Sep;94(35):e1408. doi:
10.1097/MD.0000000000001408.
PMID- 24265122
OWN - NLM
STAT- MEDLINE
DCOM- 20140513
LR - 20140324
IS - 0021-9541 (Linking)
VI - 229
IP - 7
DP - 2014 Jul
TI - Osteoactivin induces transdifferentiation of C2C12 myoblasts into
osteoblasts.
PG - 955-66
LID - 10.1002/jcp.24512 [doi]
AB - Osteoactivin (OA) is a novel osteogenic factor important for osteoblast
differentiation and function. Previous studies showed that OA stimulates
matrix
mineralization and transcription of osteoblast specific genes required for
differentiation. OA plays a role in wound healing and its expression was
shown to
increase in post fracture calluses. OA expression was reported in muscle as
OA is
upregulated in cases of denervation and unloading stress. The regulatory
mechanisms
of OA in muscle and bone have not yet been determined. In this study, we
examined
whether OA plays a role in transdifferentiation of C2C12 myoblast into
osteoblasts.
Infected C2C12 with a retroviral vector overexpressing OA under the CMV
promoter
were able to transdifferentiate from myoblasts into osteoblasts.
Immunofluorescence
analysis showed that skeletal muscle marker MF-20 was severely downregulated
in
cells overexpressing OA and contained significantly less myotubes compared to
uninfected control. C2C12 myoblasts overexpressing OA showed an increase in

expression of bone specific markers such as alkaline phosphatase and alizarin
red
staining, and also showed an increase in Runx2 protein expression. We also
detected
increased levels of phosphorylated focal adhesion kinase (FAK) in C2C12
myoblasts
overexpressing OA compared to control. Taken together, our results suggest
that OA
is able to induce transdifferentiation of myoblasts into osteoblasts through
increasing levels of phosphorylated FAK.
FAU - Sondag, Gregory R
AU - Sondag GR
AD - Department of Anatomy and Neurobiology, Northeast Ohio Medical University
(NEOMED),
Rootstown, Ohio; School of Biomedical Sciences, Kent State University, Kent,
Ohio.
FAU - Salihoglu, Sibel
AU - Salihoglu S
FAU - Lababidi, Suzanne L
AU - Lababidi SL
FAU - Crowder, Douglas C
AU - Crowder DC
FAU - Moussa, Fouad M
AU - Moussa FM
FAU - Abdelmagid, Samir M
AU - Abdelmagid SM
FAU - Safadi, Fayez F
AU - Safadi FF
LA - eng
GR - R01AR048892-06/AR/NIAMS NIH HHS/United States
PL - United States
TA - J Cell Physiol
JT - Journal of cellular physiology
JID - 0050222
RN - 0 (Core Binding Factor Alpha 1 Subunit)
RN - 0 (Eye Proteins)
RN - 0 (Gpnmb protein, mouse)
RN - 0 (Membrane Glycoproteins)
SB - IM
MH - Alkaline Phosphatase/biosynthesis
MH - Animals
MH - Cell Line
MH - Cell Transdifferentiation/*genetics
MH - Core Binding Factor Alpha 1 Subunit/biosynthesis
MH - Eye Proteins/*genetics/metabolism
MH - Focal Adhesion Protein-Tyrosine Kinases/biosynthesis
MH - Gene Expression Regulation, Developmental/genetics
MH - Humans
MH - Membrane Glycoproteins/*genetics/metabolism
MH - Mice
MH - Myoblasts/cytology/*metabolism
EDAT- 2013/11/23 06:00
MHDA- 2014/05/14 06:00
CRDT- 2013/11/23 06:00
PHST- 2013/06/04 00:00 [received]
PHST- 2013/11/18 00:00 [accepted]
PHST- 2013/11/23 06:00 [entrez]
PHST- 2013/11/23 06:00 [pubmed]
PHST- 2014/05/14 06:00 [medline]
AID - 10.1002/jcp.24512 [doi]
PST - ppublish
SO - J Cell Physiol. 2014 Jul;229(7):955-66. doi: 10.1002/jcp.24512.
PMID- 22733430
OWN - NLM
STAT- MEDLINE
DCOM- 20130507
LR - 20210108
IS - 1549-3296 (Linking)
VI - 101
IP - 1
DP - 2013 Jan
TI - Directed osteogenic differentiation of human mesenchymal stem/precursor cells
on
silicate substituted calcium phosphate.
PG - 13-22
LID - 10.1002/jbm.a.34261 [doi]
AB - Insufficient, underactive, or inappropriate osteoblast function results in
serious
clinical conditions such as osteoporosis, osteogenesis imperfecta and
fracture
nonunion and therefore the control of osteogenesis is a medical priority. In
vitro
mesenchymal stem cells (MSCs) can be directed to form osteoblasts through the
addition of soluble factors such as β-glycerophosphate, ascorbic acid, and

dexamethasone; however this is unlikely to be practical in the clinical
setting. An
alternative approach would be to use a scaffold or matrix engineered to
provide cues
for differentiation without the need for soluble factors. Here we describe
studies
using Silicate-substituted calcium phosphate (Si-CaP) and unmodified
hydroxyapatite
(HA) to test whether these materials are capable of promoting osteogenic
differentiation of MSCs in the absence of soluble factors. Si-CaP supported
attachment and proliferation of MSCs and induced osteogenesis to a greater
extent
than HA, as evidenced through upregulation of the osteoblast-related genes:
Runx2
(1.2 fold), Col1a1 (2 fold), Pth1r (1.5 fold), and Bglap (1.7 fold) Dmp1 (1.1
fold),
respectively. Osteogenic-associated proteins, alkaline phosphatase (1.4
fold),
RUNX2, COL1A1, and BGLAP, were also upregulated and there was an increased
production of mineralized bone matrix (1.75 fold), as detected by the Von
Kossa
Assay. These data indicate that inorganic substrates are capable of directing
the
differentiation programme of stem cells in the absence of known chemical
drivers and
therefore may provide the basis for bone repair in the clinical setting.
CI - Copyright © 2012 Wiley Periodicals, Inc.
FAU - Cameron, Kate
AU - Cameron K
AD - MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh,
United
Kingdom. k.r.cameron@sms.ed.ac.uk
FAU - Travers, Paul
AU - Travers P
FAU - Chander, Chaman
AU - Chander C
FAU - Buckland, Tom
AU - Buckland T
FAU - Campion, Charlie
AU - Campion C
FAU - Noble, Brendon
AU - Noble B
LA - eng
GR - CZB/4/656/CSO_/Chief Scientist Office/United Kingdom
DEP - 20120626
PL - United States
TA - J Biomed Mater Res A
JT - Journal of biomedical materials research. Part A
JID - 101234237
RN - 0 (Silicates)
SB - IM
MH - Cell Differentiation/*drug effects
MH - Humans
MH - Mesenchymal Stem Cells/*cytology/drug effects/enzymology
MH - Osteoblasts/cytology/drug effects/metabolism
MH - Silicates/*pharmacology
EDAT- 2012/06/27 06:00
MHDA- 2013/05/08 06:00
CRDT- 2012/06/27 06:00
PHST- 2012/02/28 00:00 [received]
PHST- 2012/04/13 00:00 [revised]
PHST- 2012/05/03 00:00 [accepted]
PHST- 2012/06/27 06:00 [entrez]
PHST- 2012/06/27 06:00 [pubmed]
PHST- 2013/05/08 06:00 [medline]
AID - 10.1002/jbm.a.34261 [doi]
PST - ppublish
SO - J Biomed Mater Res A. 2013 Jan;101(1):13-22. doi: 10.1002/jbm.a.34261. Epub
2012 Jun
26.
PMID- 22523967
OWN - NLM
STAT- MEDLINE
DCOM- 20120515
LR - 20190715
IS - 1533-4880 (Print)
IS - 1533-4880 (Linking)
VI - 12
IP - 1
DP - 2012 Jan
TI - Evaluation of osteoinduction and proliferation on nano-Sr-HAP: a novel
orthopedic
biomaterial for bone tissue regeneration.
PG - 207-12
AB - Hydroxyapatite (HAP), a CaP compound similar to the mineral phase present in
bone,
has excellent biocompatibility but little osseous inductivity. In this study,
we
evaluated the novel nano-Sr-HAP, in which the calcium of hydroxyapatite was
substituted with strontium, which acts as a bone-forming agent. Its
biocompatibility
and osteoinduction were assayed using marrow mesenchymal stem cells (MSCs)
and
osteoblasts (OBs) in vitro. We were able to demonstrate that nano-Sr-HAP
supported
increased OB cell adhesion, proliferation and viability up to 4 days in
culture when
compared with nano-HAP. MSCs cultured with nano-Sr-HAP showed higher alkaline
phosphatase (ALP) activity. More extracellular mineralized nodules were found

with
nano-Sr-HAP compared to nano-HAP, especially in images of ALP staining. We
suggest
that nano-Sr-HAP powders possess osteoconductive and osteoinductive
properties and
have the potential to be used in the repair of bone defects caused by
osteoporotic
fractures.
FAU - Hao, Yongqiang
AU - Hao Y
AD - Department of Orthopaedics, Ninth People's Hospital, Shanghai JiaoTong
University
School of Medicine, Shanghai 200011, China.
FAU - Yan, Huanqing
AU - Yan H
FAU - Wang, Xuepeng
AU - Wang X
FAU - Zhu, Bangshang
AU - Zhu B
FAU - Ning, Congqin
AU - Ning C
FAU - Ge, Shengfang
AU - Ge S
LA - eng
PL - United States
TA - J Nanosci Nanotechnol
JT - Journal of nanoscience and nanotechnology
JID - 101088195
SB - IM
MH - Animals
MH - Bone Substitutes/*chemical synthesis/*pharmacology
MH - Durapatite/chemistry/*pharmacology
MH - Osteoblasts/*cytology/drug effects/*physiology
MH - Rats
MH - Regeneration/*drug effects
MH - Strontium/chemistry/*pharmacology
EDAT- 2012/04/25 06:00
MHDA- 2012/05/16 06:00
CRDT- 2012/04/25 06:00
PHST- 2012/04/25 06:00 [entrez]
PHST- 2012/04/25 06:00 [pubmed]
PHST- 2012/05/16 06:00 [medline]
AID - 10.1166/jnn.2012.5125 [doi]
PST - ppublish
SO - J Nanosci Nanotechnol. 2012 Jan;12(1):207-12. doi: 10.1166/jnn.2012.5125.
PMID- 25266795
OWN - NLM
STAT- MEDLINE
DCOM- 20150217
LR - 20181113
IS - 0736-0266 (Print)
IS - 0736-0266 (Linking)
VI - 33
IP - 1
DP - 2015 Jan
TI - Parathyroid hormone 1-34 enhances extracellular matrix deposition and
organization
during flexor tendon repair.
PG - 17-24
LID - 10.1002/jor.22735 [doi]
AB - Parathyroid hormone (PTH) 1-34 is known to enhance fracture healing. Tendon
repair
is analogous to bone healing in its dependence on the proliferation and
differentiation of mesenchymal stem cells, matrix formation, and tissue
remodeling.(1,2,3) We hypothesized that PTH 1-34 enhances tendon healing in a
flexor
digitorum longus (FDL) tendon repair model. C57Bl/6J mice were treated with
either
intraperitoneal PTH 1-34 or vehicle-control (PBS). Tendons were harvested at
3-28
days for histology, gene expression, and biomechanical testing. The
metatarsophalangeal joint range of motion was reduced 1.5-2-fold in PTH 1-34
mice
compared to control mice. The gliding coefficient, a measure of adhesion
formation,
was 2-3.5-fold higher in PTH 1-34 mice. At 14 days post-repair, the tensile
strength
was twofold higher in PTH 1-34 specimens, but at 28 days there were no
differences.
PTH 1-34 mice had increased fibrous tissue deposition that correlated with
elevated
expression of collagens and fibronectin as seen on quantitative PCR. PTH 1-34
accelerated the deposition of reparative tissue but increased adhesion

formation.
FAU - Lee, Daniel J
AU - Lee DJ
AD - The Center for Musculoskeletal Research, University of Rochester Medical
Center, 601
Elmwood Avenue, Box 665, Rochester, New York, 14642.
FAU - Southgate, Richard D
AU - Southgate RD
FAU - Farhat, Youssef M
AU - Farhat YM
FAU - Loiselle, Alayna E
AU - Loiselle AE
FAU - Hammert, Warren C
AU - Hammert WC
FAU - Awad, Hani A
AU - Awad HA
FAU - O'Keefe, Regis J
AU - O'Keefe RJ
LA - eng
GR - T32 GM07356/GM/NIGMS NIH HHS/United States
GR - R01AR056696/AR/NIAMS NIH HHS/United States
GR - T32 AR053459/AR/NIAMS NIH HHS/United States
GR - T32 GM007356/GM/NIGMS NIH HHS/United States
GR - P50 AR054041/AR/NIAMS NIH HHS/United States
GR - P50AR954041/AR/NIAMS NIH HHS/United States
DEP - 20140929
TA - J Orthop Res
Research
Society
JID - 8404726
RN - 0 (Fibronectins)
SB - IM
MH - Animals
MH - Biomechanical Phenomena/drug effects/physiology
MH - Collagen/metabolism
MH - Extracellular Matrix/*drug effects/*metabolism
MH - Fibronectins/metabolism
MH - Male
MH - Metatarsophalangeal Joint/physiology
MH - Mice
MH - Models, Animal
MH - Parathyroid Hormone/*pharmacology/therapeutic use
MH - Range of Motion, Articular/drug effects/physiology
MH - Tendon Injuries/drug therapy/*metabolism/surgery
MH - Tendons/*drug effects/*metabolism/surgery
MH - Tensile Strength/drug effects/physiology
MH - Wound Healing/*drug effects/physiology
PMC - PMC4241167
MID - NIHMS624232
OTO - NOTNLM
OT - PTH 1-34
OT - adhesions
OT - biomechanics
OT - forteo
OT - tendon healing
EDAT- 2014/10/01 06:00
MHDA- 2015/02/18 06:00
CRDT- 2014/10/01 06:00
PHST- 2014/05/16 00:00 [received]
PHST- 2014/08/20 00:00 [accepted]
PHST- 2014/10/01 06:00 [entrez]
PHST- 2014/10/01 06:00 [pubmed]
PHST- 2015/02/18 06:00 [medline]
AID - 10.1002/jor.22735 [doi]
PST - ppublish
SO - J Orthop Res. 2015 Jan;33(1):17-24. doi: 10.1002/jor.22735. Epub 2014 Sep 29.
PMID- 26848820
OWN - NLM
STAT- MEDLINE
DCOM- 20180730
LR - 20180730
VI - 26
IP - 6
DP - 2017 Aug
TI - Cantilever Lengths and Anterior-Posterior Spreads of Interim, Acrylic Resin,
Full-Arch Screw-Retained Prostheses and Their Relationship to Prosthetic
Complications.
PG - 502-507
LID - 10.1111/jopr.12426 [doi]
AB - PURPOSE: To retrospectively record the distal cantilever lengths (CL) of
full-arch
interim, all-acrylic resin prostheses used in an immediate occlusal loading
protocol. Anterior/posterior (A/P) spreads were measured on master casts
associated
with the interim prostheses. Ratios were calculated (CL/AP). Prosthetic
complications were recorded. The ratios and prosthetic complications were
statistically compared and analyzed for statistical and clinical
significance.
MATERIALS AND METHODS: One hundred twenty-eight patients with 192 edentulous
arches
(109 maxillary; 83 mandibular; 190 arches were restored with 4 implants; 2
maxillary
arches were restored with 5 implants) were treated. Seven hundred seventy
implants
(Brånemark System) from September 1, 2011, until August 31, 2013 were
included in
this report. Patients were treated and followed in a single private practice
for up
to 40 months. Implants had to have at least 35 Ncm of insertion torque to be
immediately loaded. All implants were immediately loaded with full functional
occlusions on the day the implants were placed. Interim, full-arch, all-
acrylic
resin prostheses were fabricated and placed into full functional occlusion
following
an All-on-Four protocol. Measurements of the distal cantilevered segments
were made
on the prostheses prior to insertion. A/P spreads were measured on the master
casts
made from abutment level impressions made on the day of surgery. Prosthetic
complications (denture base fracture, cohesive/adhesive denture tooth
fractures)
were recorded in the charts as they occurred. All charts were reviewed for
this
report; no patients were lost to follow-up. Interim prosthetic repairs were
analyzed
by type (tooth or denture base), arch, gender, and location within the
edentulous
arches. RESULTS: One patient experienced complete maxillary implant failure;
the
overall implant survival rate (SR) was 99.5% (766 of 770). Four hundred
thirty of
434 maxillary implants and 336 of 336 mandibular implants survived for SRs of
99.1
and 100%, respectively. Thirty four of the 192 interim prostheses (17.7%)
warranted
at least one repair during treatment. The average cantilevered segments for
the
interim maxillary prostheses without prosthetic complications were 9.7 mm
(right)
and 9.5 mm (left). The average cantilevered segments for the repaired
maxillary
prostheses were 10.1 mm (right); 9.9 mm (left). The average cantilevered
segments
for the interim mandibular prostheses without prosthetic complications were
9.2 mm
(right) and 9.3 mm (left). The average cantilevered segments for the repaired
mandibular prostheses were 9.87 mm (right) and 9.18 mm (left). The average
maxillary
A/P spread was 18.4 mm; the average mandibular A/P spread was 17.3 mm. The
average
maxillary CL/AP spread ratios were 0.55 (right) and 0.53 (left); the average
mandibular CL/AP spread ratios were 0.61 (right) and 0.57 (left). There were
no
statistical correlations between the CL/AP ratios and the frequency or type
of
prosthetic repairs recorded in this study. The ratios were statistically
significant
(p = 0.041) for mandibular prostheses with prosthetic complications: slightly
greater CL/A-P ratios were noted. CONCLUSIONS: The results from this 2-year
clinical
retrospective analysis indicated that CL/AP ratios in the range of 0.5 to 0.6
generally resulted in successful interim prostheses during the time the

interim
prostheses were in function. The results of this investigation also revealed
that 1
of 129 patients experienced implant failures; implants placed and restored on
the
same day with full-arch, screw-retained prostheses resulted in high clinical
survival rates for implants and prostheses. The All-on-Four treatment
protocol used
in this study was a viable alternative to other implant loading/placement
protocols
for rehabilitating edentulous patients and resulted in minimal prosthetic
complications.
FAU - Drago, Carl
AU - Drago C
AD - Gundersen Health System, LaCrosse WI.
AD - Marquette University School of Dentistry, Milwaukee, WI.
LA - eng
DEP - 20160205
PL - United States
TA - J Prosthodont
Prosthodontists
JID - 9301275
RN - 0 (Acrylic Resins)
SB - D
MH - *Acrylic Resins
MH - Bone Screws
MH - Dental Prosthesis Design
MH - Dental Prosthesis, Implant-Supported/*adverse effects
MH - Humans
MH - Jaw, Edentulous/*surgery
MH - Postoperative Complications/*etiology
OTO - NOTNLM
OT - immediate full-arch loading
OT - interim prostheses
OT - osseointegration
EDAT- 2016/02/06 06:00
MHDA- 2018/07/31 06:00
CRDT- 2016/02/06 06:00
PHST- 2015/09/17 00:00 [accepted]
PHST- 2016/02/06 06:00 [pubmed]
PHST- 2018/07/31 06:00 [medline]
PHST- 2016/02/06 06:00 [entrez]
AID - 10.1111/jopr.12426 [doi]
PST - ppublish
SO - J Prosthodont. 2017 Aug;26(6):502-507. doi: 10.1111/jopr.12426. Epub 2016 Feb
5.
PMID- 23387447
OWN - NLM
STAT- MEDLINE
DCOM- 20130716
LR - 20131121
IS - 0167-6830 (Linking)
VI - 32
IP - 1
DP - 2013 Feb
TI - Orbital adherence with titanium mesh floor implants: a review of 10 cases.
PG - 8-11
LID - 10.3109/01676830.2012.736597 [doi]
AB - BACKGROUND: Multiple materials have been used in the repair of orbital floor
fractures. We report 10 cases of complications relating to the use of
titanium mesh
orbital floor implants. METHOD: A retrospective review of 10 cases in 2
centres in
New Zealand. Patients presented with diplopia or eyelid retraction following
repair
of an orbital floor fracture with titanium mesh implants. RESULTS: Ten
patients (7
male, 3 female) aged between 15-78 years old (mean 39 years) presented with
significant restriction of eye movement and/or eyelid retraction following
repair of
an orbital floor fracture with a titanium mesh implant. Seven patients
presented
with restriction of eye movement alone. Three patients had lower lid
retraction in
addition to restriction of eye movement. One patient presented with epiphora
following erosion of the implant through the nasolacrimal duct. Seven
patients
underwent surgical removal of the implant with all patients showing
improvement of
extraocular movement post-operatively. Three cases did not undergo implant
removal
with one case showing mild improvement over 9 months, and 2 cases showing no
improvement. The mean interval between the initial surgery and removal of the
implant was 7.1 months. DISCUSSION: In our series, 7 cases required

explantation of
the original titanium implant. In these cases a vigorous fibrotic reaction
had taken
place between the orbital contents and the titanium mesh implant. We
postulate that
the fibrous reaction between the implant and the orbital contents caused the
eye
movement restriction and the lid retraction. Implant materials used in
orbital floor
fracture surgery should be inert with a flat profile rather than a mesh to
prevent
adhesions through the mesh that may cause cicatricial eye movement
restriction and
eyelid retraction.
FAU - Kersey, Thomas L
AU - Kersey TL
AD - Department of Ophthalmology, Auckland University Hostpital, Greenlane
Clinical
Centre, Auckland, New Zealand. thomaskersey@doctors.net.uk
FAU - Ng, Stephen G J
AU - Ng SG
FAU - Rosser, Paul
AU - Rosser P
FAU - Sloan, Brian
AU - Sloan B
FAU - Hart, Richard
AU - Hart R
LA - eng
PL - England
TA - Orbit
JT - Orbit (Amsterdam, Netherlands)
JID - 8301221
SB - IM
MH - Adolescent
MH - Adult
MH - Aged
MH - Device Removal
MH - Diplopia/diagnosis/*etiology
MH - Eyelid Diseases/diagnosis/*etiology
MH - Female
MH - Fibrosis
MH - Humans
MH - Male
MH - Middle Aged
MH - Ocular Motility Disorders/diagnosis/*etiology
MH - Orbit/pathology
MH - Orbital Fractures/*surgery
MH - Reoperation
MH - Surgical Mesh/*adverse effects
MH - *Titanium
MH - Young Adult
EDAT- 2013/02/08 06:00
MHDA- 2013/07/17 06:00
CRDT- 2013/02/08 06:00
PHST- 2013/02/08 06:00 [entrez]
PHST- 2013/02/08 06:00 [pubmed]
PHST- 2013/07/17 06:00 [medline]
AID - 10.3109/01676830.2012.736597 [doi]
PST - ppublish
SO - Orbit. 2013 Feb;32(1):8-11. doi: 10.3109/01676830.2012.736597.
PMID- 26511696
OWN - NLM
STAT- MEDLINE
DCOM- 20160810
LR - 20181023
VI - 49
IP - 6
DP - 2015
TI - Effect of pentoxifylline on healing of segmental bone defects and
angiogenesis.
PG - 676-82
LID - 10.3944/AOTT.2015.15.0158 [doi]
AB - OBJECTIVE: The aim of this study was to determine the effect of
pentoxifylline (PTX)
on angiogenesis and the healing of a critical-sized segmental defect of the
radius
diaphysis in a rat model, using radiological and histological grading
systems.
METHODS: The study included 24 female Sprague-Dawley rats (weight: 300±20 g)
divided
into 4 groups. A critical-sized segmental defect was created in the radius
diaphysis
in all rats. In Group 1, morcellized iliac crest autografts were used to fill
the
segmental bone defect. In Group 2, segmental bone defects were filled using
morcellized iliac crest autografts, and 25 mg/kg/day PTX was applied
intraperitoneally. In Group 3, the segmental bone defects were not filled,
and in
Group 4 the segmental bone defects were left unfilled, and an intraperitoneal
(IP)
dose of 25 mg/kg/day PTX was applied. Rats were sacrificed at postoperative
Week 8,
and defects were evaluated using radiographic, histological and
immunohistochemical
methods. RESULTS: There were significant differences between Group 1 and 2
according
to radiological evaluation (p=0.003) and quality of union at the defect site
(p=0.01). Union quality was higher in Group 4 than Group 3 (p=0.01). Cluster
of
differentiation 31 (CD31) and vascular endothelial growth factor (VEGF)
levels were
higher in Group 2 than in Groups 3 and 4. CONCLUSION: According to
radiological and
histological parameters, PTX appears to improve angiogenesis and healing of
segmental cortical bone defects of the radius in a rat model.
FAU - Çakmak, Gökhan
AU - Çakmak G
AD - Başkent University Faculty of Medicine, Department of Orthopaedics and
Traumatology,
Antalya, Turkey. gokhancakmak75@gmail.com.
FAU - Şahin, Mehmet Şükrü
AU - Şahin MŞ
Traumatology,
Antalya, Turkey.
FAU - Özdemİr, B Handan
AU - Özdemİr BH
AD - Başkent University Faculty of Medicine, Department of Pathology, Ankara,
Turkey.
FAU - Karadenİz, Emre
AU - Karadenİz E
Traumatology,
Antalya, Turkey.
LA - eng
LA - tur
PL - Turkey
TA - Acta Orthop Traumatol Turc
JT - Acta orthopaedica et traumatologica turcica
JID - 9424806
RN - SD6QCT3TSU (Pentoxifylline)
SB - IM
MH - Animals
MH - *Bone Transplantation
MH - Bone and Bones/pathology/*surgery
MH - Diaphyses
MH - Female
MH - Pentoxifylline/*administration & dosage
MH - Radius
MH - Rats
EDAT- 2015/10/30 06:00
MHDA- 2016/08/11 06:00
CRDT- 2015/10/30 06:00
PHST- 2015/10/30 06:00 [entrez]
PHST- 2015/10/30 06:00 [pubmed]
PHST- 2016/08/11 06:00 [medline]
AID - 10.3944/AOTT.2015.15.0158 [doi]
PST - ppublish
SO - Acta Orthop Traumatol Turc. 2015;49(6):676-82. doi:
10.3944/AOTT.2015.15.0158.
PMID- 27062468
OWN - NLM
STAT- MEDLINE
DCOM- 20170202
LR - 20181202
IS - 1053-8127 (Linking)
VI - 29
IP - 4
DP - 2016 Nov 21
TI - An evaluation of meniscus tears in lateral tibial plateau fractures and
repair
results.
PG - 845-851
AB - BACKGROUND: Soft tissue injuries may co-occur with tibial plateau fractures.
These
injuries may include medial or lateral ligament ruptures, peroneal nerve
lesions,
anterior cruciate ligament ruptures, and meniscus tears. OBJECTIVE: The aim
of this
study was to investigate the frequency of meniscus tears in lateral tibial
plateau
fractures and to evaluate the clinical and radiological results of meniscus
repairs.
MATERIALS AND METHOD: The study included 19 patients who underwent surgery
for a
closed lateral tibial plateau fracture. Anteroposterior and lateral
radiographs of
the knee, followed by magnetic resonance imaging (MRI) examinations, were
undertaken
for all cases. The clinical and radiological evaluation of the surgical
treatment
results was performed according to the Rasmussen criteria. RESULTS: Meniscus
lesions
were found in 10 (52.6%) patients. Nine meniscus tears were found in patients
with
type 2 fractures, and one meniscus tear was found in a patient with a type 3
fracture. All of the menisci were separated from the peripheral capsule
adhesion
point. On the MRI examination during follow-up, all of the repaired lateral
menisci
were determined to be in their original anatomic location. CONCLUSION: For
successful outcomes in lateral plateau fractures, it is essential to
determine
whether there is a meniscus tear. In cases with meniscus tears, meniscus
repair can
be easily performed and should be considered because it has a positive impact
on the
treatment outcome.
FAU - Tekin, Ali Çağrı
AU - Tekin AÇ
AD - Orthopaedics and Traumatology Clinic, Okmeydanı Research and Training
Hospital,
İ!stanbul, Turkey.
FAU - Çakar, Murat
AU - Çakar M
Hospital,
İ!stanbul, Turkey.
FAU - Esenyel, Cem Zeki
AU - Esenyel CZ
Hospital,
İ!stanbul, Turkey.
FAU - Adaş, Müjdat
AU - Adaş M
Hospital,
İ!stanbul, Turkey.
FAU - Bayraktar, Mehmet Kürşad
AU - Bayraktar MK
Hospital,
İ!stanbul, Turkey.
FAU - Özcan, Yusuf
AU - Özcan Y
Hospital,
İ!stanbul, Turkey.
FAU - Saygılı, Mehmet Selçuk
AU - Saygılı MS
AD - M.S. Baltalimanı Bone Diseases Research and Training Hospital, İstanbul,
Turkey.
FAU - Tekin, Zeynep Nilüfer
AU - Tekin ZN
AD - Radiology Clinic, Darıca Farabi Government Hospital, Kocaeli, Turkey.
LA - eng
PL - Netherlands
TA - J Back Musculoskelet Rehabil
JT - Journal of back and musculoskeletal rehabilitation
JID - 9201340
SB - IM
MH - Adult
MH - Female
MH - Humans
MH - Male
MH - Middle Aged
MH - Pain Measurement
MH - Tibial Fractures/classification/diagnostic imaging/*surgery
MH - Tibial Meniscus Injuries/diagnostic imaging/*surgery
MH - Young Adult
OTO - NOTNLM
OT - Knee joint
OT - intra-articular fractures
OT - menisci
OT - tibial
EDAT- 2016/04/12 06:00
MHDA- 2017/02/06 06:00
CRDT- 2016/04/11 06:00
PHST- 2016/04/12 06:00 [pubmed]
PHST- 2017/02/06 06:00 [medline]
PHST- 2016/04/11 06:00 [entrez]
AID - BMR698 [pii]
AID - 10.3233/BMR-160698 [doi]
PST - ppublish
SO - J Back Musculoskelet Rehabil. 2016 Nov 21;29(4):845-851. doi: 10.3233/BMR-
160698.
PMID- 26346217
OWN - NLM
STAT- MEDLINE
DCOM- 20160811
LR - 20190108
IS - 2045-2322 (Linking)
VI - 5
DP - 2015 Sep 8
TI - Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous
composite scaffolds--an in vitro efficacy study.
PG - 13775
LID - 10.1038/srep13775 [doi]
LID - 13775
AB - Bone infections are common in trauma-induced open fractures with bone
defects.
Therefore, developing anti-infection scaffolds for repairing bone defects is
desirable. This study develoepd novel Mg-based porous composite scaffolds
with a
basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium
phosphate (TCP). A unique low-temperature rapid prototyping technology was
used to
fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M),
PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion
and
biofilm formation of Staphylococcus aureus were evaluated. The results
indicated
that the Mg-based scaffolds significantly inhibited bacterial adhesion and
biofilm
formation compared to PT, and the PT10M and PT15M exhibited significantly
stronger
anti-biofilm ability than PT5M. In vitro degratation tests revealed that the
degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+)
concentration
and osmolality, and the increased pH may be one of the major contributing
factors to
the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M
exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1

cells.
In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and
biofilm formation, and the PT10M scaffold was considered to be an effective

composition with considerable antibacterial ability and good
cytocompatibility.
FAU - Ma, Rui
AU - Ma R
AD - Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic
Surgery,
Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of
Medicine,
Shanghai, China.
AD - Department of Orthopedic Surgery, the Second Affiliated Hospital of Xi'an
Jiaotong
University, Xi'an, Shanxi Province, China.
FAU - Lai, Yu-xiao
AU - Lai YX
AD - Translational Medicine Research and Development Centre, Institute of
Biomedical and
Health Engineering, Shenzhen Institute of Advanced Technology, The Chinese
Academy
of Sciences, Shenzhen, China.
FAU - Li, Long
AU - Li L
Biomedical and
Academy
FAU - Tan, Hong-lue
AU - Tan HL
Surgery,
Medicine,
Shanghai, China.
FAU - Wang, Jia-li
AU - Wang JL
AD - Department of Orthopedics and Traumatology, The Chinese University of Hong
Kong,
Hong Kong, China.
FAU - Li, Ye
AU - Li Y
Biomedical and
Academy
FAU - Tang, Ting-ting
AU - Tang TT
Surgery,
Medicine,
Shanghai, China.
FAU - Qin, Ling
AU - Qin L
Biomedical and
Academy
AD - Department of Orthopedics and Traumatology, The Chinese University of Hong
Kong,
Hong Kong, China.
LA - eng
DEP - 20150908
TA - Sci Rep
JID - 101563288
RN - K4C08XP666 (tricalcium phosphate)
SB - IM
MH - Animals
MH - *Anti-Bacterial Agents
MH - Bacterial Adhesion
MH - Biofilms
MH - *Bone Substitutes/chemistry
MH - Calcium Phosphates/chemistry
MH - Cell Adhesion
MH - Cell Line
MH - *Magnesium/chemistry
MH - Mice
MH - Microbial Sensitivity Tests
MH - Microbial Viability
MH - Polyglycolic Acid/chemistry
MH - Staphylococcus aureus/drug effects/ultrastructure
MH - *Tissue Scaffolds/chemistry
PMC - PMC4561899
EDAT- 2015/09/09 06:00
MHDA- 2016/08/12 06:00
CRDT- 2015/09/09 06:00
PHST- 2015/03/18 00:00 [received]
PHST- 2015/08/05 00:00 [accepted]
PHST- 2015/09/09 06:00 [entrez]
PHST- 2015/09/09 06:00 [pubmed]
PHST- 2016/08/12 06:00 [medline]
AID - 10.1038/srep13775 [doi]
PST - epublish
SO - Sci Rep. 2015 Sep 8;5:13775. doi: 10.1038/srep13775.
PMID- 29806260
OWN - NLM
STAT- MEDLINE
DCOM- 20180629
LR - 20190402
IS - 1002-1892 (Print)
IS - 1002-1892 (Linking)
VI - 31
IP - 3
DP - 2017 Mar 15
TI - [Evaluation of closed multi-axial screws iliosacral fixation system combined
with
posterior segmental spinal fixation for treatment of unstable sacral
fractures].
PG - 313-318
LID - 10.7507/1002-1892.201608014 [doi]
AB - OBJECTIVE: To evaluate the effectiveness of lumbopelvic fixation using the
combination of closed multi-axial screws (CMAS) iliosacral fixation system
and the
posterior segmental spinal fixation for unstable sacral fractures. METHODS:
Between
January 2013 and November 2014, 25 patients (39 sides) with unstable sacral
fractures were treated with lumbopelvic fixation using the combination of
CMAS
iliosacral fixation system and the posterior segmental spinal fixation. There
were
17 males and 8 females, aged 19-55 years (mean, 33.9 years). The causes were
traffic
accident injury in 15 cases, falling injury from height in 8 cases, and
crushing
injury in 2 cases. The interval of injury and operation was 1-13 days (mean,
3.5
days). Fracture was classified as Denis type I in 2 sides, type II in 20
sides, and
type III in 17 sides; nerve injury was rated as Gibbons grade I in 2 cases,
grade II
in 2 cases, grade III in 7 cases, and grade IV in 9 cases. The reduction
quality was
evaluated by Matta criterion, the clinical function outcome by Majeed, and
nerve
function by Gibbons criterion. RESULTS: The average operation time was 110
minutes
(range, 80-150 minutes). The average blood loss was 570 mL (range, 250-1 400
mL).
Superficial wound infection occurred in 2 patients, and was cured after
debridement
and antibiotic therapy. All patients were followed up for an average of 18
months
(range, 15-22 months). Postoperative X-ray and CT examination showed clinical
healing of sacral fractures at 8-12 weeks after operation (mean, 10 weeks).

The mean
removal time of internal fixation was 13 months (range, 12-20 months). No
screw
loosening and fracture, adhesion of internal fixation to surrounding tissue,
and
obvious electrolysis phenomenon occurred. According to Matta criterion,
reduction
was rated as excellent in 32 sides, good in 6 sides, fair in 1 side, and the
excellent and good rate was 97.5%. According to Majeed functional scoring at
last
follow-up, the mean score was 84.7 (range, 64-98); the results were excellent
in 18
cases, good in 5 cases, and fair in 2 cases, and the excellent and good rate
was
92.0%. The nerve function was significantly improved when compared with
preoperative
one; nerve injury was rated as Gibbons grade I in 8 cases, grade II in 8
cases,
grade III in 3 cases, and grade IV in 1 case. CONCLUSION: Lumbopelvic
fixation using
the combination of CMAS iliosacral fixation system and the posterior
segmental
spinal fixation is a relatively effective fixation for unstable sacral
fractures.
Not only is the fracture fixation rigid for early full weight-bearing, but
also
nerve decompression can be performed which facilitates nerve function
recovery.
FAU - Huang, Zhong
AU - Huang Z
AD - Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu
Sichuan, 610041, P.R.China.
FAU - Meng, Weikun
AU - Meng W
FAU - Li, Liang
AU - Li L
FAU - Tan, Zhen
AU - Tan Z
FAU - Guo, Qiang
AU - Guo Q
FAU - Liu, Lei
AU - Liu L
FAU - Huang, Fuguo
AU - Huang F
FAU - Wang, Guanglin
AU - Wang G
Sichuan, 610041, P.R.China.wglfrank@163.com.
LA - chi
PL - China
zazhi =
JID - 9425194
SB - IM
MH - Adult
MH - *Bone Screws
MH - Female
MH - *Fracture Fixation, Internal
MH - Fractures, Bone
MH - Humans
MH - Male
MH - Middle Aged
MH - Sacrum/*injuries
MH - Spinal Fractures/*surgery
MH - Young Adult
OTO - NOTNLM
OT - *Sacral fracture
OT - *closed multi-axial screws
OT - *lumbopelvic fixation
OT - *pelvic fracture
EDAT- 2017/03/01 00:00
MHDA- 2018/06/30 06:00
CRDT- 2018/05/29 06:00
PHST- 2018/05/29 06:00 [entrez]
PHST- 2017/03/01 00:00 [pubmed]
PHST- 2018/06/30 06:00 [medline]
AID - 10.7507/1002-1892.201608014 [doi]
PST - ppublish
SO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2017 Mar 15;31(3):313-318. doi:
10.7507/1002-1892.201608014.
PMID- 26455161
OWN - NLM
STAT- MEDLINE
DCOM- 20160302
LR - 20181202
IS - 1002-1892 (Print)
IS - 1002-1892 (Linking)
VI - 29
IP - 1
DP - 2015 Jan
TI - [ARTHROSCOPOIC FIXATION WITH PERCUTANEOUS CANNULATED SCREWS FOR ACUTE
DISPLACED
ISOLATED GREATER TUBEROSITY FRACTURES OF THE PROXIMAL HUMERUS].
PG - 1-5
AB - OBJECTIVE: To evaluate the technique and the effectiveness of arthroscopic
fixation
with percutaneous cannulated screws for acute displaced isolated greater
tuberosity
fractures of the proximal humerus. METHODS: A retrospective analysis was made
on the
clinical data of 15 patients with acute displaced isolated greater tuberosity
fractures of the proximal humerus, who accepted arthroscopic percutaneous

cannulated
screw fixation treatment between January 2010 and February 2013. There were 8
males
and 7 females with an average age of 44.9 years (range, 31-66 years). Eight
left
shoulders and 7 right shoulders were affected. The mean interval of injury
and
operation was 9.9 days (range, 4-19 days). Before operation, the average
range of
motion (ROM) values of the affected shoulder were (74.13 ±17.19)° in forward
flexion, (121.67 ± 17.50)° in abduction, (T11 ± 2)° in internal rotation, and
(39.27
± 8.08)° in external rotation; the visual analogue scale (VAS) score was 6.46
±
1.30; and Costant score was 62.27 ± 11.90. RESULTS: Operations were all
successfully
completed and incision healed by first intention in all cases. All cases were
followed up 12-27 months (mean, 15 months). Postoperative X-ray films showed

good
reduction, alignment, and union of fracture. The average fracture healing
time was 8
weeks (range, 6-13 weeks). At last follow-up, the ROM of the affected
shoulder were
significantly improved to (169.33 ± 7.99)° in forward flexion, (156.67 ±
10.47)° in
abduction, (T6 ± 2)° in internal rotation, and (71.67 ± 7.94)° in external
rotation
(P < 0.05); the VAS score was significantly reduced to 1.73 ± 1.02 (t =
-8.51, P =
0.00); and the Costant score was significantly increased to 96.20 ± 2.34 (t =
11.50,
P = 0.00). No complication was found, such as neural or vascular injury,
infection,
shoulder joint adhesion, fixation failure, or fracture displacement.
CONCLUSION:
Arthroscopic fixation with percutaneous cannulated screws is a safe method to
treat
acute displaced isolated greater tuberosity fractures of the proximal
humerus, and
it has good short-term effectiveness with the advantages of little trauma and
satisfactory functional recovery.

FAU - Xi, Zhijie
AU - Xi Z
FAU - Liang, Qianqian
AU - Liang Q
FAU - Mi, Kun
AU - Mi K
FAU - Feng, Zhibin
AU - Feng Z
FAU - Wang, Bin
AU - Wang B
LA - chi
PL - China
zazhi =
JID - 9425194
SB - IM
MH - Adult
MH - Aged
MH - *Bone Screws
MH - Epiphyses
MH - Female
MH - Humans
MH - Humerus/*surgery
MH - Male
MH - Middle Aged
MH - Shoulder
MH - Shoulder Joint
EDAT- 2015/10/13 06:00
MHDA- 2016/03/05 06:00
CRDT- 2015/10/13 06:00
PHST- 2015/10/13 06:00 [entrez]
PHST- 2015/10/13 06:00 [pubmed]
PHST- 2016/03/05 06:00 [medline]
PST - ppublish
SO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2015 Jan;29(1):1-5.
PMID- 25953935
OWN - NLM
STAT- MEDLINE
DCOM- 20160211
LR - 20150508
IS - 1512-0112 (Print)
IS - 1512-0112 (Linking)
IP - 241
DP - 2015 Apr
TI - [Immunological aspects of delayed regeneration of mandibular fractures].
PG - 30-6
AB - The level of complications in patients with the mandibular fractures does not
have a
tendency to the decline. A research purposes is a study of the basic laws of
immunological reactions and possibility of optimizing processes osteogenesis
by
drugs-cytokines at patients with the mandibular fractures with delayed
consolidation
of bone tissue. 46 patients with the mandibular fractures were observed. The
maintenance of cytokines IL - 1β, TNF - α, IL - 4, SICAM-1 in the blood
serum, IgA,
IgM, IgG in a mouth liquid was probed. It is set that in pathogenesis of
delayed
consolidation a basic role is played by changes reactivity of organism, which
realized in three directions: immunodeficit of humoral immunity,

immunodepression of
cellular factors of defence, disbalance in functioning of the cytokines
system. It
is necessary to count the levels of products SICAM-1 and cytokine IL-1β in
the blood
serum by the diagnostic criteria of bone repair features at patients with the
mandibular fractures: development of delayed consolidation of mandibular

fragments
is accompanied the increase of their parameters at the control group more
than in 2
and in 15 times (668,2±10,3 pg/ml and 363,4 ±6,6 pg/ml relatively).
Includding in
the complex treatment of the mandibular fractures of immunomodulator
Ronkoleukin
showed clinico-immunological efficiency for the patients with impaired bone
repair.
FAU - Mashchenko, I
AU - Mashchenko I
AD - SE «Dnipropetrovsk medical academy Ministry of Health of Ukraine»,
Dnipropetrovsk,
Ukraine.
FAU - Idashkina, N
AU - Idashkina N
Dnipropetrovsk,
Ukraine.
FAU - Gudaryan, A
AU - Gudaryan A
Dnipropetrovsk,
Ukraine.
LA - rus
PL - Georgia (Republic)
TA - Georgian Med News
JT - Georgian medical news
JID - 101218222
RN - 0 (Immunoglobulin A)
RN - 0 (Immunoglobulin G)
RN - 0 (Immunoglobulin M)
RN - 0 (Immunologic Factors)
RN - 0 (Interleukin-1beta)
RN - 0 (Tumor Necrosis Factor-alpha)
RN - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN - 207137-56-2 (Interleukin-4)
SB - IM
MH - Adult
MH - Bone Regeneration/drug effects/*immunology
MH - Female
MH - Humans
MH - Immunoglobulin A/immunology
MH - Immunoglobulin G/immunology
MH - Immunoglobulin M/immunology
MH - Immunologic Factors/administration & dosage
MH - Intercellular Adhesion Molecule-1/blood/immunology
MH - Interleukin-1beta/blood/immunology
MH - Interleukin-4/blood/immunology
MH - Male
MH - Mandibular Fractures/blood/*drug therapy/*immunology
MH - Osteogenesis/drug effects/*immunology
MH - Tumor Necrosis Factor-alpha/blood/immunology
EDAT- 2015/05/09 06:00
MHDA- 2016/02/13 06:00
CRDT- 2015/05/09 06:00
PHST- 2015/05/09 06:00 [entrez]
PHST- 2015/05/09 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
PST - ppublish
SO - Georgian Med News. 2015 Apr;(241):30-6.
PMID- 24124594
OWN - NLM
STAT- MEDLINE
DCOM- 20140522
LR - 20181113
IS - 1932-6203 (Linking)
VI - 8
IP - 10
DP - 2013
TI - Sonic hedgehog regulates osteoblast function by focal adhesion kinase
signaling in
the process of fracture healing.
PG - e76785
LID - e76785
AB - Several biological studies have indicated that hedgehog signaling plays an
important
role in osteoblast proliferation and differentiation, and sonic hedgehog
(SHH)
expression is positively correlated with phosphorylated focal adhesion kinase
(FAK)
Tyr(397). However, the relationship between them and their role in the
process of
normal fracture repair has not been clarified yet. Immunohistochemical
analysis
revealed that SHH and pFAK Tyr(397) were expressed in bone marrow cells and
that
pFAK Tyr(397) was also detected in ALP-positive osteoblasts near the TRAP-
positive
osteoclasts in the fracture site in the ribs of mice on day 5 after fracture.
SHH
and pFAK Tyr(397) were detectable in osteoblasts near the hypertrophic
chondrocytes
on day 14. In vitro analysis showed that SHH up-regulated the expression of
FAK mRNA
and pFAK Tyr(397) time dependently in osteoblastic MC3T3-E1 cells. Functional
analysis revealed that 5 lentivirus encoding short hairpin FAK RNAs (shFAK)-
infected
MC3T3-E1 cell groups displayed a round morphology and decreased
proliferation,
adhesion, migration, and differentiation. SHH stimulated the proliferation
and
differentiation of MC3T3-E1 cells, but had no effect on the shFAK-infected
cells.
SHH also stimulated osteoclast formation in a co-culture system containing
MC3T3-E1
and murine CD11b(+) bone marrow cells, but did not affect the shFAK-infected
MC3T3-E1 co-culture group. These data suggest that SHH signaling was
activated in
osteoblasts at the dynamic remodeling site of a bone fracture and regulated
their
proliferation and differentiation, as well as osteoclast formation, via FAK
signaling.
FAU - Horikiri, Yuu
AU - Horikiri Y
AD - Department of Oral and Maxillofacial Surgery, Okayama University Graduate
School of
Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
FAU - Shimo, Tsuyoshi
AU - Shimo T
FAU - Kurio, Naito
AU - Kurio N
FAU - Okui, Tatsuo
AU - Okui T
FAU - Matsumoto, Kenichi
AU - Matsumoto K
FAU - Iwamoto, Masahiro
AU - Iwamoto M
FAU - Sasaki, Akira
AU - Sasaki A
LA - eng
DEP - 20131004
TA - PLoS One
JT - PloS one
JID - 101285081
RN - 0 (Hedgehog Proteins)
RN - 0 (Parathyroid Hormone-Related Protein)
SB - IM
MH - Animals
MH - Cell Adhesion/genetics
MH - Cell Differentiation/genetics
MH - Cell Line
MH - Cell Movement/genetics
MH - Focal Adhesion Protein-Tyrosine Kinases/genetics/*metabolism
MH - Gene Knockdown Techniques
MH - Hedgehog Proteins/genetics/*metabolism
MH - Male
MH - Mice
MH - Osteoclasts/drug effects/metabolism
MH - Parathyroid Hormone-Related Protein/metabolism/pharmacology
MH - RNA Interference
MH - Rib Fractures/genetics/metabolism/pathology
MH - *Signal Transduction
PMC - PMC3790742
exist.
EDAT- 2013/10/15 06:00
MHDA- 2014/05/23 06:00
CRDT- 2013/10/15 06:00
PHST- 2013/05/04 00:00 [received]
PHST- 2013/09/03 00:00 [accepted]
PHST- 2013/10/15 06:00 [entrez]
PHST- 2013/10/15 06:00 [pubmed]
PHST- 2014/05/23 06:00 [medline]
PST - epublish
SO - PLoS One. 2013 Oct 4;8(10):e76785. doi: 10.1371/journal.pone.0076785.
eCollection
2013.
PMID- 29457418
OWN - NLM
STAT- MEDLINE
DCOM- 20180702
LR - 20181202
IS - 1003-0034 (Print)
IS - 1003-0034 (Linking)
VI - 30
IP - 10
DP - 2017 Oct 25
TI - [Outcomes and complications of Tightrope button plate for repairing
acromioclavicular dislocation].
PG - 946-951
LID - 10.3969/j.issn.1003-0034.2017.10.013 [doi]
AB - OBJECTIVE: To study the clinical outcome and complications of Tightrope
button plate
for repairing acromioclavicular dislocation of Rockwood type III to V.
METHODS: From
May 2014 to December 2016, 17 patients with acromioclavicular dislocation of
type
III-V were treated with Tightrope button plate including 10 males and 7
females with
an average age 39.8 years old ranging from 20 to 68 years old. Four patients
were
treated with arthroscopy and 17 patients were treated with mini-invasive by
X-ray
assisted. Shoulder function, X-ray and complications after operation were
assessed.
RESULTS: All patients were followed up for 5 to 23 months with a mean of 10.8
months. All patients got satisfying reduction immediately postoperatively.

Among
them, 1 case of clavicle end wound foreign body reaction, rupture, effusion,
healing
after the second suture; 1 case of foreign body granuloma formation at the
end of
clavicle were resected and removed at 4 months after operation; 3 cases loss
reduction(less than 50% of acromioclavicular joint). No coracoid fracture and
suture
breakage observed. The shoulder mobility was restored in 15 cases at 4 to 6
weeks
postoperatively, and the shoulder adhesion in 2 cases was delayed to 5 to 7
months
after operation. The Constant scores were improved from 46.9±6.0
preoperatively to
92.7±4.0 at the final follow-up. X-ray evaluation of postoperative
coracoclavicular
tunnel location, patients' coracoclavicular tunnel with mini-invasive
fluoroscopy
all closed to the ideal position (across the clavicle vertically through the
coracoid base center), while different degree of tunnel position deviation
were
observed in arthroscopic patients. CONCLUSIONS: Tightrope button plate for
the
treatment of acromioclavicular joint dislocation had advantages of minimally
invasive, effective, good clinical results, the majority of common
complications
does not affect efficacy. Small incision X-ray method can provide more
satisfactory
and reliable tunnel location.
FAU - Zuo, Yong-Xiang
AU - Zuo YX
AD - Department of Orthopaedics, The First People's Hospital of Wenling Affiliated
to
Wenzhou Medical College, Wenling 317500, Zhejiang, China; 513168774@qq.com.
FAU - Ma, Zi-Ping
AU - Ma ZP
AD - Department of Orthopaedics, The First People's Hospital of Wenling Affiliated
to
Wenzhou Medical College, Wenling 317500, Zhejiang, China.
LA - chi
PL - China
TA - Zhongguo Gu Shang
JT - Zhongguo gu shang = China journal of orthopaedics and traumatology
JID - 9815790
SB - IM
MH - Acromioclavicular Joint/*injuries
MH - Adult
MH - Aged
MH - Bone Plates/*adverse effects
MH - Clavicle
MH - Female
MH - Humans
MH - Joint Dislocations
MH - Male
MH - Middle Aged
MH - Shoulder Dislocation/*surgery
MH - Young Adult
OTO - NOTNLM
OT - Acromioclavicular joint
OT - Arthroscopes
OT - Dislocations
OT - Postoperative complications
COIS- The authors of this article and the planning committee members and staff have
no
relevant financial relationships with commercial interests to disclose.
EDAT- 2018/02/20 06:00
MHDA- 2018/07/03 06:00
CRDT- 2018/02/20 06:00
PHST- 2017/06/13 00:00 [received]
PHST- 2018/02/20 06:00 [entrez]
PHST- 2018/02/20 06:00 [pubmed]
PHST- 2018/07/03 06:00 [medline]
AID - 10.3969/j.issn.1003-0034.2017.10.013 [doi]
PST - ppublish
SO - Zhongguo Gu Shang. 2017 Oct 25;30(10):946-951. doi:
10.3969/j.issn.1003-0034.2017.10.013.
PMID- 22570220
OWN - NLM
STAT- MEDLINE
DCOM- 20120703
LR - 20181201
IS - 0736-0266 (Linking)
VI - 30
IP - 7
DP - 2012 Jul
TI - Comparison of the osteogenic capacity of minipig and human bone marrow-
derived
mesenchymal stem cells.
PG - 1019-25
LID - 10.1002/jor.22049 [doi]
AB - Minipigs are a recommended large animal model for preclinical testing of
human
orthopedic implants. Mesenchymal stem cells (MSCs) are the key repair cells
in bone
healing and implant osseointegration, but the osteogenic capacity of minipig
MSCs is
incompletely known. The aim of this study was to isolate and characterize
minipig
bone marrow (BM) and peripheral blood (PB) MSCs in comparison to human BM-
MSCs. BM
sample was aspirated from posterior iliac crest of five male Göttingen
minipigs (age
15 ± 1 months). PB sample was drawn for isolation of circulating MSCs. MSCs
were
selected by plastic-adherence as originally described by Friedenstein. Cell
morphology, colony formation, proliferation, surface marker expression, and
differentiation were examined. Human BM-MSCs were isolated and cultured from
adult
fracture patients (n = 13, age 19-60 years) using identical techniques. MSCs
were
found in all minipig BM samples, but no circulating MSCs could be detected.
Minipig
BM-MSCs had similar morphology, proliferation, and colony formation
capacities as
human BM-MSCs. Unexpectedly, minipig BM-MSCs had a significantly lower
ability than
human BM-MSCs to form differentiated and functional osteoblasts. This
observation
emphasizes the need for species-specific optimization of MSC culture protocol
before
direct systematic comparison of MSCs between human and various preclinical
large
animal models can be made.
FAU - Heino, Terhi J
AU - Heino TJ
AD - Orthopaedic Research Unit, University of Turku, Turku, Finland.
terhi.j.heino@utu.fi
FAU - Alm, Jessica J
AU - Alm JJ
FAU - Moritz, Niko
AU - Moritz N
FAU - Aro, Hannu T
AU - Aro HT
LA - eng
DEP - 20120103
PL - United States
TA - J Orthop Res
Research
Society
JID - 8404726
RN - 0 (Biomarkers)
RN - 0 (Plastics)
SB - IM
MH - Adipocytes/*cytology/metabolism
MH - Adolescent
MH - Adult
MH - Animals
MH - Biomarkers/metabolism
MH - Bone Marrow Cells/*cytology
MH - Cell Culture Techniques
MH - Cell Differentiation/physiology
MH - Cryopreservation
MH - Female
MH - Humans
MH - Male
MH - Mesenchymal Stem Cells/*cytology/metabolism
MH - Middle Aged
MH - Osteoblasts/*cytology/metabolism
MH - Plastics
MH - Species Specificity
MH - Swine
MH - Swine, Miniature
MH - Young Adult
EDAT- 2012/05/10 06:00
MHDA- 2012/07/04 06:00
CRDT- 2012/05/10 06:00
PHST- 2011/06/29 00:00 [received]
PHST- 2011/12/05 00:00 [accepted]
PHST- 2012/05/10 06:00 [entrez]
PHST- 2012/05/10 06:00 [pubmed]
PHST- 2012/07/04 06:00 [medline]
AID - 10.1002/jor.22049 [doi]
PST - ppublish
SO - J Orthop Res. 2012 Jul;30(7):1019-25. doi: 10.1002/jor.22049. Epub 2012 Jan
3.
PMID- 23567945
OWN - NLM
STAT- MEDLINE
DCOM- 20131017
LR - 20161125
IS - 1742-7061 (Linking)
VI - 9
IP - 7
DP - 2013 Jul
TI - Bi-layer collagen/microporous electrospun nanofiber scaffold improves the
osteochondral regeneration.
PG - 7236-47
LID - S1742-7061(13)00175-X [pii]
LID - 10.1016/j.actbio.2013.04.003 [doi]
AB - An optimal scaffold is crucial for osteochondral regeneration. Collagen and
electrospun nanofibers have been demonstrated to facilitate cartilage and
bone
regeneration, respectively. However, the effect of combining collagen and
electrospun nanofibers on osteochondral regeneration has yet to be evaluated.
Here,
we report that the combination of collagen and electrospun poly-l-lactic acid
nanofibers synergistically promotes osteochondral regeneration. We first

fabricated
bi-layer microporous scaffold with collagen and electrospun poly-l-lactic
acid
nanofibers (COL-nanofiber). Mesenchymal stem cells were cultured on the bi-
layer
scaffold and their adhesion, proliferation and differentiation were examined.
Moreover, osteochondral defects were created in rabbits and implanted with

COL-nanofiber scaffold. Cartilage and subchondral bone regeneration were
evaluated
at 6 and 12weeks after surgery. Compared with COL scaffold, cells on COL-
nanofiber
scaffold exhibited more robust osteogenic differentiation, indicated by
higher
expression levels of OCN and runx2 genes as well as the accumulation of
calcium
nodules. Furthermore, implantation of COL-nanofiber scaffold seeded with
cells
induced more rapid subchondral bone emergence, and better cartilage
formation, which
led to better functional repair of osteochondral defects as manifested by
histological staining, biomechanical test and micro-computed tomography data.
Our
study underscores the potential of using the bi-layer microporous COL-
nanofiber
scaffold for the treatment of deep osteochondral defects.
reserved.
FAU - Zhang, Shufang
AU - Zhang S
AD - Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang
University, Hangzhou, Zhejiang, People's Republic of China.
FAU - Chen, Longkun
AU - Chen L
FAU - Jiang, Yangzi
AU - Jiang Y
FAU - Cai, Youzhi
AU - Cai Y
FAU - Xu, Guowei
AU - Xu G
FAU - Tong, Tong
AU - Tong T
FAU - Zhang, Wei
AU - Zhang W
FAU - Wang, Linlin
AU - Wang L
FAU - Ji, Junfeng
AU - Ji J
FAU - Shi, Peihua
AU - Shi P
FAU - Ouyang, Hong Wei
AU - Ouyang HW
LA - eng
DEP - 20130406
PL - England
TA - Acta Biomater
JID - 101233144
RN - 0 (Polyesters)
RN - 0 (Polymers)
SB - IM
MH - Animals
MH - Chondrogenesis/physiology
MH - Collagen/*chemistry
MH - Fractures, Cartilage/pathology/*physiopathology/*surgery
MH - Guided Tissue Regeneration/*instrumentation
MH - Male
MH - Mesenchymal Stem Cell Transplantation/*instrumentation
MH - Nanofibers/*chemistry/ultrastructure
MH - Polyesters
MH - Polymers/chemistry
MH - Porosity
MH - Rabbits
MH - Regeneration/physiology
MH - Swine
EDAT- 2013/04/10 06:00
MHDA- 2013/10/18 06:00
CRDT- 2013/04/10 06:00
PHST- 2012/10/17 00:00 [received]
PHST- 2013/03/20 00:00 [revised]
PHST- 2013/04/01 00:00 [accepted]
PHST- 2013/04/10 06:00 [entrez]
PHST- 2013/04/10 06:00 [pubmed]
PHST- 2013/10/18 06:00 [medline]
AID - S1742-7061(13)00175-X [pii]
AID - 10.1016/j.actbio.2013.04.003 [doi]
PST - ppublish
SO - Acta Biomater. 2013 Jul;9(7):7236-47. doi: 10.1016/j.actbio.2013.04.003. Epub
2013
Apr 6.
PMID- 22848334
OWN - NLM
DCOM- 20120823
LR - 20200929
IS - 1874-1207 (Linking)
VI - 6
DP - 2012
TI - Hybrid matrix grafts to favor tissue regeneration in rabbit femur bone
lesions.
PG - 85-91
LID - 10.2174/1874120701206010085 [doi]
AB - At present, typical approaches employed to repair fractures and other bone
lesions
tend to use matrix grafts to promote tissue regeneration. These grafts act as
templates, which promote cellular adhesion, growth and proliferation,

osteoconduction, and even osteoinduction, which commonly results in de novo
osteogenesis. The present work aimed to study the bone-repairing ability of
hybrid
matrixes (HM) prepared with polyvinyl alcohol (PVA) and bioactive glass in an
experimental rabbit model. The HM were prepared by combining 30% bioactive

glass
(nominal composition of 58% SiO2 -33 % CaO - 9% P2O5) and 70% PVA. New
Zealand
rabbits were randomly divided into the control group (C group) and two groups
with
bone lesions, in which one received a matrix implant HM (Implant group),
while the
other did not (no Implant group). Clinical monitoring showed no altered
parameters
from either the Implant or the no Implant groups as compared to the control
group,
for the variables of diet grades, day and night temperatures and hemograms.
In the
Implant group, radiologic and tomographic studies showed implanted areas with
clean
edges in femoral non-articular direction, and radio-dense images that suggest
incipient integration. Minimum signs of phlogosis could be observed, whereas

no
signs of rejection at this imaging level could be identified. Histological
analysis
showed evidence of osteo-integration, with the formation of a trabecular bone
within
the implant. Together, these results show that implants of hybrid matrixes of
bioactive glass are capable of promoting bone regeneration.

FAU - Goy, Dante Pascual
AU - Goy DP
AD - LABOATEM: Fac Cs Médicas, Universidad Nacional de Rosario, Argentina.
FAU - Gorosito, Emmanuel
AU - Gorosito E
FAU - Costa, Hermes S
AU - Costa HS
FAU - Mortarino, Pablo
AU - Mortarino P
FAU - Pedemonte, Noelia Acosta
AU - Pedemonte NA
FAU - Toledo, Javier
AU - Toledo J
FAU - Mansur, Herman S
AU - Mansur HS
FAU - Pereira, Marivalda M
AU - Pereira MM
FAU - Battaglino, Ricardo
AU - Battaglino R
FAU - Feldman, Sara
AU - Feldman S
LA - eng
DEP - 20120710
TA - Open Biomed Eng J
JT - The open biomedical engineering journal
JID - 101507900
PMC - PMC3406269
OTO - NOTNLM
OT - femur bone lesion.
OT - hybrid matrix
OT - polyvinyl alcohol
EDAT- 2012/08/01 06:00
MHDA- 2012/08/01 06:01
CRDT- 2012/08/01 06:00
PHST- 2012/03/08 00:00 [received]
PHST- 2012/05/15 00:00 [revised]
PHST- 2012/05/29 00:00 [accepted]
PHST- 2012/08/01 06:00 [entrez]
PHST- 2012/08/01 06:00 [pubmed]
PHST- 2012/08/01 06:01 [medline]
AID - TOBEJ-6-85 [pii]
AID - 10.2174/1874120701206010085 [doi]
PST - ppublish
SO - Open Biomed Eng J. 2012;6:85-91. doi: 10.2174/1874120701206010085. Epub 2012
Jul 10.
PMID- 22218382
OWN - NLM
STAT- MEDLINE
DCOM- 20120221
LR - 20160512
IS - 0021-9355 (Linking)
VI - 94
IP - 1
DP - 2012 Jan 4
TI - Temporal and spatial vascularization patterns of unions and nonunions: role
of
vascular endothelial growth factor and bone morphogenetic proteins.
PG - 49-58
LID - 10.2106/JBJS.J.00795 [doi]
AB - BACKGROUND: Failure of fracture-healing with nonunion is a major clinical
problem.
Angiogenesis is closely linked to bone regeneration, but the role of
angiogenesis in
nonunion formation remains unclear. Because established nonunions are well
vascularized, we hypothesized that lack of vascular endothelial growth factor
(VEGF)
expression and vascularization during the early time course of fracture-
healing
determine nonunion formation. METHODS: In seventy-two CD-1 mice, a femoral
osteotomy
with a gap size of 1.80 mm (nonunion group) or a gap size of 0.25 mm (union
group)
was created and stabilized by a pin-clip technique. Healing was analyzed
after
three, seven, fourteen, twenty-one, twenty-eight, and seventy days by micro-
computed
tomography and histomorphometry. Vascularization was determined in different
healing
zones by immunohistochemical staining of PECAM-1 (platelet-endothelial cell
adhesion
molecule). Additional animals were analyzed after seven, fourteen, and
twenty-one
days with Western blot analysis of VEGF, bone morphogenetic protein (BMP)-2,
and
BMP-4 expression. RESULTS: Micro-computed tomography and histomorphometry
showed
complete bone-bridging in the union group, whereas animals in the nonunion
group
showed atrophic nonunion formation. Vascularization increased from day 3 to
day 7 in
both groups, with a subsequent decrease after fourteen days. However, overall
vascularization did not differ between unions and nonunions over time. It is
of
interest that vascularization within the endosteal healing zone was even
higher in
nonunions than in unions after fourteen days. Expression of VEGF was
significantly
higher in nonunions, while expression of BMP-2 and 4 and proliferating cell
nuclear
antigen were found significantly reduced compared with unions. CONCLUSIONS:
Because
vascularization during the early time course of fracture-healing was not
impaired
despite the failure of bone-healing in nonunions, we rejected our hypothesis
and
accepted the null hypothesis that nonunion formation is not due to failure of
VEGF-mediated angiogenesis. Failure of fracture-healing was associated with a
decreased expression of BMP-2 and 4 and a disturbed ratio of angiogenic to

osteogenic growth factors, which may be responsible for nonunion. CLINICAL
RELEVANCE: Because the intrinsic angiogenic response during nonunion
formation was
sufficient for adequate vascularization, treatment strategies for nonunions
should
focus on the stimulation of osteogenesis rather than on the stimulation of
angiogenesis.
FAU - Garcia, P
AU - Garcia P
AD - Department of Trauma, Hand and Reconstructive Surgery, Collaborative Research
Center, AO Foundation, University of Saarland, D-66421 Homburg/Saar, Germany.
patric.garcia@uks.eu
FAU - Pieruschka, A
AU - Pieruschka A
FAU - Klein, M
AU - Klein M
FAU - Tami, A
AU - Tami A
FAU - Histing, T
AU - Histing T
FAU - Holstein, J H
AU - Holstein JH
FAU - Scheuer, C
AU - Scheuer C
FAU - Pohlemann, T
AU - Pohlemann T
FAU - Menger, M D
AU - Menger MD
LA - eng
PL - United States
TA - J Bone Joint Surg Am
JT - The Journal of bone and joint surgery. American volume
JID - 0014030
RN - 0 (Bone Morphogenetic Proteins)
SB - AIM
SB - IM
MH - Animals
MH - Bone Morphogenetic Proteins/*physiology
MH - Fractures, Ununited/*etiology
MH - Mice
MH - Time Factors
MH - Vascular Endothelial Growth Factor A/*physiology
EDAT- 2012/01/06 06:00
MHDA- 2012/02/22 06:00
CRDT- 2012/01/06 06:00
PHST- 2012/01/06 06:00 [entrez]
PHST- 2012/01/06 06:00 [pubmed]
PHST- 2012/02/22 06:00 [medline]
AID - 10.2106/JBJS.J.00795 [doi]
PST - ppublish
SO - J Bone Joint Surg Am. 2012 Jan 4;94(1):49-58. doi: 10.2106/JBJS.J.00795.
PMID- 27026212
OWN - NLM
STAT- MEDLINE
DCOM- 20170728
LR - 20200810
IS - 1883-1958 (Linking)
VI - 60
IP - 4
DP - 2016 Oct
TI - The effect of low-intensity pulsed ultrasound on wound healing using scratch
assay
in epithelial cells.
PG - 308-314
LID - S1883-1958(16)30001-9 [pii]
LID - 10.1016/j.jpor.2016.03.002 [doi]
AB - PURPOSE: Low-intensity pulsed ultrasound (LIPUS) is widely used in medical
fields
because it shortens the time required for biologic wound healing in fracture
treatment. Also, in dental fields, LIPUS should be effectively employed for
implant
treatment. However, most of the relevant reports have been published on its
effects
on bone formation around implants, and the effects of LIPUS on soft tissue
healing
remain unclear. In the present study, we examined the effects of LIPUS on
soft
tissue healing using gingival epithelial cells. METHODS: Gingival epithelial
cells
were cultured on a dish, followed by LIPUS exposure at a frequency of 3MHz
for
15min. The cells were counted with a hemocytometer, and a scratch assay was
conducted by measuring the closing area of the scratch wound using a
microscope.
Following LIPUS exposure, total RNA was collected for microarray analysis. In
addition, real-time PCR was performed to examine the mRNA expression level of
integrin α6β4. Furthermore, total protein was collected to examine the

protein
expression level of integrin α6β4 by western blotting. RESULTS: The cell
count and
scratch assay demonstrated that LIPUS exposure promoted cell proliferation
and
scratch-wound closure. Microarray analysis demonstrated the increased
expression
levels of adhesion-related genes, including integrin. Real-time PCR analysis
demonstrated that LIPUS exposure significantly up-regulated the mRNA
expression
level of integrin α6β4. Western blotting showed intense staining of integrin
α6β4.
CONCLUSION: LIPUS exposure promotes wound closure in the scratch assay and
up-regulates the expression level of integrin α6β4 as compared with the
control.
CI - Copyright © 2016 Japan Prosthodontic Society. Published by Elsevier Ltd. All
rights
reserved.
FAU - Iwanabe, Yujiro
AU - Iwanabe Y
AD - Department of Oral Reconstruction and Rehabilitation, Kyushu Dental
University,
Japan.
FAU - Masaki, Chihiro
AU - Masaki C
University,
Japan. Electronic address: masaki@kyu-dent.ac.jp.
FAU - Tamura, Akiko
AU - Tamura A
University,
Japan.
FAU - Tsuka, Shintaro
AU - Tsuka S
University,
Japan.
FAU - Mukaibo, Taro
AU - Mukaibo T
University,
Japan.
FAU - Kondo, Yusuke
AU - Kondo Y
University,
Japan.
FAU - Hosokawa, Ryuji
AU - Hosokawa R
University,
Japan.
LA - eng
DEP - 20160326
PL - Japan
TA - J Prosthodont Res
JT - Journal of prosthodontic research
JID - 101490359
RN - 0 (Integrin alpha6beta4)
RN - 0 (RNA, Messenger)
SB - D
SB - IM
MH - Animals
MH - Cell Adhesion/genetics
MH - Epithelial Cells/*physiology
MH - Gingiva/cytology
MH - Integrin alpha6beta4/genetics/metabolism
MH - RNA, Messenger
MH - *Ultrasonic Therapy
MH - *Ultrasonic Waves
MH - Up-Regulation
MH - *Wound Healing/genetics
OTO - NOTNLM
OT - Integrin α6β4
OT - LIPUS
OT - Wound healing
EDAT- 2016/10/25 06:00
MHDA- 2017/07/29 06:00
CRDT- 2016/03/31 06:00
PHST- 2016/01/27 00:00 [received]
PHST- 2016/03/07 00:00 [revised]
PHST- 2016/03/15 00:00 [accepted]
PHST- 2016/10/25 06:00 [pubmed]
PHST- 2017/07/29 06:00 [medline]
PHST- 2016/03/31 06:00 [entrez]
AID - S1883-1958(16)30001-9 [pii]
AID - 10.1016/j.jpor.2016.03.002 [doi]
PST - ppublish
SO - J Prosthodont Res. 2016 Oct;60(4):308-314. doi: 10.1016/j.jpor.2016.03.002.
Epub
2016 Mar 26.
PMID- 23124266
OWN - NLM
STAT- MEDLINE
DCOM- 20130701
LR - 20130114
IS - 0362-2436 (Linking)
VI - 38
IP - 2
DP - 2013 Jan 15
TI - Cyclic-RGD is as effective as rhBMP-2 in anterior interbody fusion of the
sheep
cervical spine.
PG - E59-65
LID - 10.1097/BRS.0b013e31827ad2ff [doi]
AB - STUDY DESIGN: Radiological and histological assessment of fusion status after
anterior cervical discectomy and fusion (ACDF) procedure in a sheep spinal

fusion
model. OBJECTIVE: To evaluate the efficacy of cyclic arginine-glycine-
aspartic
(cRGD) in comparison with recombinant human bone morphogenetic protein-2
(rhBMP-2)
on a mineralized collagen matrix (MCM). SUMMARY OF BACKGROUND DATA: A
previous
evaluation of MCM alone in comparison with autologous bone graft alone was
not able
to show an advantage on spinal fusion. The cRGD peptide sequence plays a
major role
in mediating cell adhesion. Studies have demonstrated enhances osteoblasts
adhesion
resulting in increased periimplant bone formation after implantcoating with
cRGD.
rhBMP-2 has already proven its ability to enhance spinal fusion. To date, no
comparative in vivo evaluation of cRGD and rhBMP-2 in combination with a MCM
for
spinal fusion has been performed. METHODS: Twenty-four sheep (N = 8/group)
underwent
C3-C4 fusion. Implants: group 1: titanium cage with MCM and rhBMP-2; group 2:
titanium cage with MCM and cRGD; control group: titanium cage with MCM alone.
After
12 weeks fusion sites were evaluated by computed tomography to assess fusion
status,
bone mineral density as well as bony callus volume. Furthermore,
histomorphological
and histomorphometrical analysis of the fusion sites were performed. RESULTS:
In
comparison with the control group, cRGD, and rhBMP-2 groups showed a higher
fusion
rate in radiographical findings and a higher degree of interbody fusion in
histomorphometrical analysis (P < 0.05). There was no significant difference
in
radiographical and histological parameters between the rhBMP-2 and the cRGD
group.
Although rhBMP-2 demonstrated ectopic prevertebral bone formations, this
effect was
less prominent in the cRGD group. CONCLUSION: In this animal model the
combination
of cRGD and a mineralized collagen matrix showed superior fusion results in
comparison with the mineralized collagen alone. Further, cRGD was comparably
effective to rhBMP-2 in promoting interbody fusion by demonstrating less
ectopic
bone formations.
FAU - Scholz, Matti
AU - Scholz M
AD - Center for Spinal Surgery and Neurotraumatology, Berufsgenossenschaftliche
Unfallklinik Frankfurt am Main, Friedberger Landstraße 430, Frankfurt am
Main,
Germany. matti.scholz@bgu-frankfurt.de
FAU - Schleicher, Philipp
AU - Schleicher P
FAU - Sewing, Andreas
AU - Sewing A
FAU - Gelinsky, Michael
AU - Gelinsky M
FAU - Kandziora, Frank
AU - Kandziora F
LA - eng
PL - United States
TA - Spine (Phila Pa 1976)
JT - Spine
JID - 7610646
RN - 0 (Peptides, Cyclic)
RN - 0 (Transforming Growth Factor beta)
RN - 0 (cyclic arginine-glycine-aspartic acid peptide)
RN - 0 (recombinant human bone morphogenetic protein-2)
SB - IM
MH - Animals
MH - Bone Matrix
MH - Bone Morphogenetic Protein 2/*pharmacology
MH - Bone Plates
MH - Cervical Vertebrae/*drug effects/pathology/surgery
MH - Collagen/administration & dosage
MH - Diskectomy
MH - Drug Therapy, Combination
MH - Female
MH - Osteoblasts/drug effects/pathology
MH - Peptides, Cyclic/*pharmacology
MH - Sheep
MH - Spinal Fusion/instrumentation/*methods
MH - Transforming Growth Factor beta/*pharmacology
EDAT- 2012/11/06 06:00
MHDA- 2013/07/03 06:00
CRDT- 2012/11/06 06:00
PHST- 2012/11/06 06:00 [entrez]
PHST- 2012/11/06 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
AID - 10.1097/BRS.0b013e31827ad2ff [doi]
PST - ppublish
SO - Spine (Phila Pa 1976). 2013 Jan 15;38(2):E59-65. doi:
10.1097/BRS.0b013e31827ad2ff.
PMID- 21776677
OWN - NLM
STAT- MEDLINE
DCOM- 20110823
LR - 20190715
IS - 1533-4880 (Print)
IS - 1533-4880 (Linking)
VI - 11
IP - 4
DP - 2011 Apr
TI - A new growth factor controlled drug release system to promote healing of bone
fractures: nanospheres of recombinant human bone morphogenetic-2 and

polylactic
acid.
PG - 3107-14
AB - To prepare a new drug control release system, which can markedly promote the
healing
of bone fractures. Optimized water-in-oil-in-water multiple emulsion
evaporation
method, prepared nanospheres of recombinant human bone morphogenetic-2 and
polylactic acid (rhBMP-2-PLA-Ns). Its physical character was determined by
the
enzyme linked immunosorbent assay method. Its bioactivity was measured with
the
microculture tetrazolium test immunohistochemical analyses, alizarin red
staining
and western blot analysis. rhBMP-2-PLA-Ns exhibited an even and uniform
spherical
appearance without adhesion, with a particle size distribution between 35 and
65 nm,
and a mean size of 45 nm. The drug loading volume and encapsulation
efficiency
reached ([124.73 +/- 0.41] x 10(-3))% and (90.54 +/- 1.32)%, respectively.
The drug
release in vitro persisted for 14 days, with a mean concentration of 73.44
+/- 5.38
ng/ml, and corresponded to the Higuichi equation (r = 0.9962). The
microculture
tetrazolium test showed that 4 days later, the optical density value ranking
was
rhBMP-2-PLA-N group > rhBMP-2 group > blank control group. Fluorescence
immunocytochemical analysis showed that 10 days later the fluorescent density
of the
rhBMP-2-PLA-N group was significantly higher than the other two groups.
Western blot
analysis confirmed that the amount of vascular endothelial growth factor in
the
rhBMP-2-PLA-N group was the greatest. This study showed that rhBMP-2-PLA-Ns
have
excellent biological activity, can promote proliferation, differentiation and
mineralization of osteoblasts. The drug release time is suitable for fracture
healing and is an ideal delivery system for fracture healing.

FAU - Chen, Lin
AU - Chen L
AD - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology,
Sichuan
University, Chengdu 610041, P.R. China.
FAU - Liu, Lei
AU - Liu L
FAU - Li, Cai
AU - Li C
FAU - Tan, Yinghui
AU - Tan Y
FAU - Zhang, Gang
AU - Zhang G
LA - eng
PL - United States
TA - J Nanosci Nanotechnol
JT - Journal of nanoscience and nanotechnology
JID - 101088195
RN - 0 (Delayed-Action Preparations)
RN - 0 (Nanocapsules)
RN - 0 (Polyesters)
RN - 0 (Polymers)
SB - IM
MH - Bone Morphogenetic Protein 2/chemistry/genetics/*pharmacology
MH - Cell Line
MH - Delayed-Action Preparations/chemistry/*pharmacology
MH - Fractures, Bone/drug therapy
MH - Humans
MH - Intercellular Signaling Peptides and Proteins/chemistry/pharmacology
MH - Lactic Acid/*chemistry
MH - Nanocapsules/*chemistry
MH - Osteoblasts/*drug effects/*physiology
MH - Polyesters
MH - Polymers/*chemistry
MH - Recombinant Proteins/chemistry/pharmacology
EDAT- 2011/07/23 06:00
MHDA- 2011/08/24 06:00
CRDT- 2011/07/23 06:00
PHST- 2011/07/23 06:00 [entrez]
PHST- 2011/07/23 06:00 [pubmed]
PHST- 2011/08/24 06:00 [medline]
AID - 10.1166/jnn.2011.3820 [doi]
PST - ppublish
SO - J Nanosci Nanotechnol. 2011 Apr;11(4):3107-14. doi: 10.1166/jnn.2011.3820.
PMID- 26695113
OWN - NLM
STAT- MEDLINE
DCOM- 20160928
LR - 20181202
IS - 1748-6041 (Linking)
VI - 11
IP - 1
DP - 2015 Dec 23
TI - Fabrication and characterization of a novel carbon fiber-reinforced calcium
phosphate silicate bone cement with potential osteo-inductivity.
PG - 015003
LID - 10.1088/1748-6041/11/1/015003 [doi]
AB - The repair of bone defects is still a pressing challenge in clinics.
Injectable bone
cement is regarded as a promising material to solve this problem because of
its
special self-setting property. Unfortunately, its poor mechanical
conformability,
unfavorable osteo-genesis ability and insufficient osteo-inductivity
seriously limit
its clinical application. In this study, novel experimental calcium phosphate
silicate bone cement reinforced by carbon fibers (CCPSC) was fabricated and
characterized. First, a compressive strength test and cell culture study were
carried out. Then, the material was implanted into the femoral epiphysis of
beagle
dogs to further assess its osteo-conductivity using a micro-computed
tomography scan
and histological analysis. In addition, we implanted CCPSC into the beagles'
intramuscular pouches to perform an elementary investigation of its
osteo-inductivity. The results showed that incorporation of carbon fibers
significantly improved its mechanical properties. Meanwhile, CCPSC had better
biocompatibility to activate cell adhesion as well as proliferation than

poly-methyl
methacrylate bone cement based on the cell culture study. Moreover,
pronounced
biodegradability and improved osteo-conductivity of CCPSC could be observed
through
the in vivo animal study. Finally, a small amount of osteoid was found at the
heterotopic site one month after implantation which indicated potential

osteo-inductivity of CCPSC. In conclusion, the novel CCPSC shows promise as a
bioactive bone substitute in certain load-bearing circumstances.

FAU - Zheng, Jiangjiang
AU - Zheng J
AD - State Key Laboratory of Oral Diseases, West China School of Stomatology,
Sichuan
University, Chengdu, Sichuan 610041, People's Republic of China.
FAU - Xiao, Yu
AU - Xiao Y
FAU - Gong, Tianxing
AU - Gong T
FAU - Zhou, Shuxin
AU - Zhou S
FAU - Troczynski, Tom
AU - Troczynski T
FAU - Yang, Quanzu
AU - Yang Q
FAU - Bao, Chongyun
AU - Bao C
FAU - Xu, Xiaoming
AU - Xu X
LA - eng
DEP - 20151223
PL - England
TA - Biomed Mater
JID - 101285195
RN - 0 (Carbon Fiber)
RN - 0 (Silicates)
RN - 7440-44-0 (Carbon)
RN - 9011-14-7 (Polymethyl Methacrylate)
SB - IM
MH - Animals
MH - Bone Cements/*chemical synthesis/*therapeutic use
MH - Carbon/*chemistry
MH - Carbon Fiber
MH - Dogs
MH - Femoral Fractures/pathology/*therapy
MH - Male
MH - Polymethyl Methacrylate/chemistry
MH - Silicates/chemistry/therapeutic use
EDAT- 2015/12/24 06:00
MHDA- 2016/09/30 06:00
CRDT- 2015/12/24 06:00
PHST- 2015/12/24 06:00 [entrez]
PHST- 2015/12/24 06:00 [pubmed]
PHST- 2016/09/30 06:00 [medline]
AID - 10.1088/1748-6041/11/1/015003 [doi]
PST - epublish
SO - Biomed Mater. 2015 Dec 23;11(1):015003. doi: 10.1088/1748-6041/11/1/015003.
PMID- 24167618
OWN - NLM
STAT- MEDLINE
DCOM- 20140528
LR - 20190226
IS - 1932-6203 (Linking)
VI - 8
IP - 10
DP - 2013
TI - Periostin deficiency increases bone damage and impairs injury response to
fatigue
loading in adult mice.
PG - e78347
LID - e78347
AB - Bone damage removal and callus formation in response to fatigue loading are
essential to prevent fractures. Periostin (Postn) is a matricellular protein
that
mediates adaptive response of cortical bone to loading. Whether and how
periostin
influences damage and the injury response to fatigue remains unknown. We
investigated the skeletal response of Postn(-/-) and Postn(+/+) mice after
fatigue
stimulus by axial compression of their tibia. In Postn(+/+) mice, cracks
number and
surface (CsNb, CsS) increased 1h after fatigue, with a decrease in strength
compared
to non-fatigued tibia. At 15 days, CsNb had started to decline, while CtTV
and CtBV
increased in fatigued vs non-fatigued tibia, reflecting a woven bone response
that
was present in 75% of the fatigued bones. Cortical porosity and remodelling
also
prominently increased in the fatigued tibia of Postn(+/+) mice. At 30 days,
paralleling a continuous removal of cortical damage, strength of the fatigued
tibia
was similar to the non-fatigue tibia. In Postn(-/-) mice, cracks were
detectable
even in the absence of fatigue, while the amount of collagen crosslinks and
tissue
hardness was decreased compared to Postn(+/+). Fatigue significantly
increased CsNb
and CsS in Postn(-/-), but was not associated with changes in CtTV and CtBV,
as only
16% of the fatigued bones formed some woven bone. Cortical porosity and
remodelling
did not increase either after fatigue in Postn(-/-), and the level of damage
remained high even after 30 days. As a result, strength remained compromised
in
Postn(-/-) mice. Contrary to Postn(+/+), which osteocytic lacunae showed a
change in
the degree of anisotropy (DA) after fatigue, Postn(-/-) showed no DA change.
Hence
periostin appears to influence bone materials properties, damage accumulation
and
repair, including local modeling/remodeling processes in response to fatigue.
These
observations suggest that the level of periostin expression could influence
the
propensity to fatigue fractures.
FAU - Bonnet, Nicolas
AU - Bonnet N
Geneva
University Hospital, Geneva, Switzerland.
FAU - Gineyts, Evelyne
AU - Gineyts E
FAU - Ammann, Patrick
AU - Ammann P
FAU - Conway, Simon J
AU - Conway SJ
FAU - Garnero, Patrick
AU - Garnero P
FAU - Ferrari, Serge
AU - Ferrari S
LA - eng
DEP - 20131022
TA - PLoS One
JT - PloS one
JID - 101285081
RN - 0 (Postn protein, mouse)
SB - IM
MH - Animals
MH - *Bone Remodeling
MH - Cell Adhesion Molecules/genetics/*metabolism
MH - Fractures, Stress/genetics/*metabolism/pathology
MH - Mice
MH - Mice, Knockout
MH - Osteocytes/*metabolism/pathology
MH - Time Factors
MH - Weight-Bearing
PMC - PMC3805534
exist.
EDAT- 2013/10/30 06:00
MHDA- 2014/05/29 06:00
CRDT- 2013/10/30 06:00
PHST- 2013/07/04 00:00 [received]
PHST- 2013/09/16 00:00 [accepted]
PHST- 2013/10/30 06:00 [entrez]
PHST- 2013/10/30 06:00 [pubmed]
PHST- 2014/05/29 06:00 [medline]
PST - epublish
SO - PLoS One. 2013 Oct 22;8(10):e78347. doi: 10.1371/journal.pone.0078347.
eCollection
2013.
PMID- 25726945
OWN - NLM
STAT- MEDLINE
DCOM- 20170703
LR - 20170703
IS - 0143-2885 (Linking)
VI - 49
IP - 3
DP - 2016 Mar
TI - Intentional replantation of adhesively reattached vertically fractured
maxillary
single-rooted teeth.
PG - 227-36
LID - 10.1111/iej.12444 [doi]
AB - AIM: To evaluate the clinical outcomes of intentionally replanted maxillary
single-rooted teeth with vertical root fractures (VRFs) after being repaired
extraorally using 4-methacryloxyethyl trimellitate
anhydride/methacrylate-tri-n-butyl borane (4-META/MMA-TBB) resin cement.
METHODOLOGY: Twenty-one root filled maxillary single-rooted teeth with VRFs
were
evaluated. After atraumatic extraction, fractured fragments were adhesively
cemented. The teeth were then replanted and splinted to the neighbouring
teeth for 2
weeks. Plaque index (PI), gingival index (GI), probing depth (PD) and
clinical
attachment level (CAL) were assessed at baseline, 6 and 12 months, and
radiographic
evaluations were made using PAI scores at baseline and 12 months. Mobility
was
evaluated using periotest values (PTV) at baseline, 1, 3, 6 and 12 months.
Replanted
teeth, contralateral teeth (control teeth) and adjacent teeth were analysed
statistically using repeated measures one-way anova, unpaired t-tests and
Wilcoxon
matched-pairs signed-rank tests. RESULTS: Two teeth were extracted in the
first
month after surgery. PI, GI, CAL and PD scores of the replanted teeth were
significantly lower at 6 month (P < 0.0001 for all) and 12 month (P < 0.0001
for
all) postoperatively when compared to baseline, but the values were not
significantly different from those of the control and adjacent teeth. PTV of
the
test teeth increased significantly (P < 0.0001) after the intervention and
decreased
to baseline levels by month 12. PTVs were significantly higher (P < 0.05) at
baseline, 1, 3 and 6 months in the test teeth when compared with the control
teeth,
but were not significantly different at month 12. PAI scores of teeth with
VRF were
significantly lower (P < 0.05) at 12 months compared with baseline.
CONCLUSIONS:
Adhesive cementation and intentional replantation were an effective treatment
modality for this group of vertically fractured maxillary single-rooted

teeth. The
clinical periodontal parameters decrease by month 6, and the mobility
returned to
the physiological limits of natural teeth 12 months after replantation.
CI - © 2015 International Endodontic Journal. Published by John Wiley & Sons Ltd.
FAU - Nizam, N
AU - Nizam N
AD - Department of Periodontology, School of Dentistry, Turkey.
FAU - Kaval, M E
AU - Kaval ME
AD - Department of Endodontology, School of Dentistry, Ege University, Izmir,
Turkey.
FAU - Gürlek, Ö
AU - Gürlek Ö
FAU - Atila, A
AU - Atila A
FAU - Çalışkan, M K
AU - Çalışkan MK
AD - Department of Endodontology, School of Dentistry, Ege University, Izmir,
Turkey.
LA - eng
DEP - 20150316
PL - England
TA - Int Endod J
JT - International endodontic journal
JID - 8004996
RN - 0 (Boron Compounds)
RN - 0 (Methylmethacrylates)
RN - 95508-14-8 (Super-bond)
SB - D
MH - Adult
MH - Boron Compounds/*therapeutic use
MH - Dental Plaque Index
MH - Female
MH - Humans
MH - Male
MH - Maxilla
MH - Methacrylates/*therapeutic use
MH - Methylmethacrylates/*therapeutic use
MH - Middle Aged
MH - Periodontal Index
MH - Resin Cements/*therapeutic use
MH - Root Canal Therapy
MH - Tooth Replantation/*methods
OTO - NOTNLM
OT - adhesive bonding
OT - intentional replantation
OT - periodontal healing
OT - vertical root fracture
EDAT- 2015/03/03 06:00
MHDA- 2017/07/04 06:00
CRDT- 2015/03/03 06:00
PHST- 2014/04/14 00:00 [received]
PHST- 2015/02/25 00:00 [accepted]
PHST- 2015/03/03 06:00 [entrez]
PHST- 2015/03/03 06:00 [pubmed]
PHST- 2017/07/04 06:00 [medline]
AID - 10.1111/iej.12444 [doi]
PST - ppublish
SO - Int Endod J. 2016 Mar;49(3):227-36. doi: 10.1111/iej.12444. Epub 2015 Mar 16.
PMID- 29577100
OWN - NLM
LR - 20201001
IS - 2451-9936 (Linking)
VI - 9
DP - 2018 Mar
TI - Lagophthalmos caused by cicatricial adhesion of orbital adipose tissue to
orbital
roof: A case report.
PG - 99-102
LID - 10.1016/j.ajoc.2018.01.014 [doi]
AB - PURPOSE: To report a case of lagophthalmos caused by cicatricial adhesion of
orbital
adipose tissue to the orbital roof. OBSERVATIONS: A 23-year-old female was
presented
with right lagophthalmos. Five months prior to consult at our clinic, she
suffered
from a penetrating trauma to the frontal lobe of the brain through the right
orbital
roof with cerebrospinal fluid leakage. Decompressive craniectomy was
performed
immediately after the injury using a coronal incision, which was followed by
reconstruction with an artificial bone 1 month later. On examination at our
clinic,
she showed right exposure keratopathy with best corrected visual acuity of
20/100
due to corneal opacity. The palpebral contracted scar was first elongated
using
Z-plasty technique but excursion of the upper eyelid under a finger force
assistance
was insufficient to eliminate lagophthalmos. However, complete eyelid closure
under
a finger force assistance was achieved after sharp dissection of the
cicatrized
adipose tissue from the orbital roof. An autogenous dermis-fat was grafted on
the
orbital roof and superior orbital rim to avoid adhesion of orbital adipose
tissue
onto the bone again. CONCLUSION AND IMPORTANCE: Cicatricial adhesion of an
orbital
adipose tissue to the orbital roof is one of the possible causes of
posttraumatic
lagophthalmos in patients with an orbital roof fracture. Surgeons need to be
aware
of this condition in planning of surgical repair when such a fracture is
encountered.
FAU - Kitaguchi, Yoshiyuki
AU - Kitaguchi Y
AD - Department of Oculoplastic, Orbital & Lacrimal Surgery, Aichi Medical
University
Hospital, Aichi, Japan.
FAU - Mupas-Uy, Jacqueline
AU - Mupas-Uy J
University
FAU - Takahashi, Yasuhiro
AU - Takahashi Y
University
FAU - Kakizaki, Hirohiko
AU - Kakizaki H
University
LA - eng
PT - Case Reports
DEP - 20180106
TA - Am J Ophthalmol Case Rep
JT - American journal of ophthalmology case reports
JID - 101679941
PMC - PMC5862542
OTO - NOTNLM
OT - Cicatricial adhesion
OT - Dermis-fat graft
OT - Lagophthalmos
OT - Orbital adipose tissue
OT - Orbital roof
EDAT- 2018/03/27 06:00
MHDA- 2018/03/27 06:01
CRDT- 2018/03/27 06:00
PHST- 2017/03/18 00:00 [received]
PHST- 2018/01/02 00:00 [revised]
PHST- 2018/01/03 00:00 [accepted]
PHST- 2018/03/27 06:00 [entrez]
PHST- 2018/03/27 06:00 [pubmed]
PHST- 2018/03/27 06:01 [medline]
AID - S2451-9936(17)30068-3 [pii]
AID - 10.1016/j.ajoc.2018.01.014 [doi]
PST - epublish
SO - Am J Ophthalmol Case Rep. 2018 Jan 6;9:99-102. doi:
10.1016/j.ajoc.2018.01.014.
eCollection 2018 Mar.
PMID- 27484679
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR - 20181113
IS - 0934-6694 (Linking)
VI - 28
IP - 5
DP - 2016 Oct
TI - [Osteosynthesis of displaced fractures of the greater tuberosity with the
Bamberg
plate].
PG - 392-401
LID - 10.1007/s00064-016-0462-8 [doi]
AB - OBJECTIVE: Internal fixation of displaced fractures of the greater tuberosity
allowing functional aftercare. INDICATIONS: Displaced fractures of the

greater
tuberosity >5 mm. Displaced fractures of the greater tuberosity >3 mm in
athletes or
overhead workers. Multiply fragmented fractures of the greater tuberosity.
CONTRAINDICATIONS: Displaced 3- or 4-part fractures of the proximal humerus.
Nondisplaced fractures of the greater tuberosity. SURGICAL TECHNIQUE:
Exposure of
the fracture of the greater tuberosity by an anterolateral approach. Open
reduction
and temporary retention with a Kirschner wire or a "Kugelspieß" or
reinforcement of
the supraspinatus tendon and distal retention. Bending and positioning of the
Bamberg plate and fixation by conventional or locking screws. Optional

fixation of
the rotator cuff to the plate. Exact monitoring of the implant position using
the
image intensifier to avoid inadequate distalization of the greater
tuberosity.
POSTOPERATIVE MANAGEMENT: Arm sling (e. g. Gilchrist) for 2 weeks. Start
passive
assisted exercise on postoperative day 1. Movement allowed up to the pain
threshold.
Physiotherapeutic treatment to prevent adhesions and capsular shrinking.
RESULTS: In
all, 10 patients with displaced fractures of the greater tuberosity underwent
osteosynthesis using the Bamberg plate. After a follow-up of at least

6 months,
a Constant-Murley score of 94.2 points (range 91-98 points) was achieved.
The
patients' average age was 45.6 years (range 29-68 years).
FAU - Popp, D
AU - Popp D
AD - Klinik für Orthopädie und Unfallchirurgie Sektion Sportorthopädie,
Sporttraumatologie, Sportmedizin und Chirurgie der oberen Extremität,
Sozialstiftung
Bamberg, Klinikum am Bruderwald, Buger Straße 80, 96049, Bamberg,
Deutschland.
dominik.popp@sozialstiftung-bamberg.de.
FAU - Schöffl, V
AU - Schöffl V
Sozialstiftung
Deutschland.
FAU - Strecker, W
AU - Strecker W
Sozialstiftung
Deutschland.
LA - ger
PT - Clinical Trial
TT - Osteosynthese dislozierter Tuberculum-majus-Frakturen mit der Bamberger
Platte.
DEP - 20160802
PL - Germany
TA - Oper Orthop Traumatol
JT - Operative Orthopadie und Traumatologie
JID - 9604937
SB - IM
MH - Adult
MH - Aged
MH - *Bone Plates
MH - Fracture Fixation, Internal/*instrumentation/methods
MH - Fractures, Comminuted/diagnosis/*surgery
MH - Humans
MH - Middle Aged
MH - Minimally Invasive Surgical Procedures/*instrumentation/methods
MH - Shoulder Fractures/diagnosis/*surgery
OTO - NOTNLM
OT - *Bone plate
OT - *Fixed-angle implant
OT - *Humeral fracture
OT - *Minimally invasive surgical procedures
OT - *Prostheses and implants
EDAT- 2016/08/04 06:00
MHDA- 2017/04/07 06:00
CRDT- 2016/08/04 06:00
PHST- 2015/04/22 00:00 [received]
PHST- 2016/02/23 00:00 [accepted]
PHST- 2016/02/02 00:00 [revised]
PHST- 2016/08/04 06:00 [entrez]
PHST- 2016/08/04 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
AID - 10.1007/s00064-016-0462-8 [pii]
AID - 10.1007/s00064-016-0462-8 [doi]
PST - ppublish
SO - Oper Orthop Traumatol. 2016 Oct;28(5):392-401. doi: 10.1007/s00064-016-0462-
8. Epub
2016 Aug 2.
PMID- 28487939
OWN - NLM
STAT- MEDLINE
DCOM- 20180305
LR - 20181113
IS - 1791-2997 (Print)
IS - 1791-2997 (Linking)
VI - 15
IP - 6
DP - 2017 Jun
TI - Identification of novel genes associated with fracture healing in
osteoporosis
induced by Krm2 overexpression or Lrp5 deficiency.
PG - 3969-3976
LID - 10.3892/mmr.2017.6544 [doi]
AB - The aim of the present study was to screen potential key genes associated
with
osteoporotic fracture healing. The microarray data from the Gene Expression
Omnibus
database accession number GSE51686, were downloaded and used to identify
differentially expressed genes (DEGs) in fracture callus tissue samples
obtained
from the femora of type I collagen (Col1a1)-kringle containing transmembrane
protein 2 (Krm2) mice and low density lipoprotein receptor-related
protein 5-/-
(Lrp5-/-) transgenic mice of osteoporosis compared with those in wild-type
(WT)
mice. Enrichment analysis was performed to reveal the DEG function. In
addition,
protein-protein interactions (PPIs) of DEGs were analyzed using the Search
Tool for
the Retrieval of Interacting Genes database. The coexpression associations
between
hub genes in the PPI network were investigated, and a coexpression network
was
constructed. A total of 841 DEGs (335 upregulated and 506 downregulated) were
identified in the Col1a1-Krm2 vs. the WT group, and 50 DEGs (16 upregulated
and 34
downregulated) were identified in the Lrp5-/- vs. the WT group. The DEGs in
Col1a1-Krm2 mice were primarily associated with immunity and cell adhesion
(GO:
0007155) functions. By contrast, the DEGs in Lrp5-/- mice were significantly
associated with muscle system process (GO: 0003012) and regulation of
transcription
(GO: 0006355). In addition, a series of DEGs demonstrated a higher score in
the PPI
network, and were observed to be coexpressed in the coexpression network, and
included thrombospondin 2 (Thbs2), syndecan 2 (Sdc2), FK506 binding protein

10
(Fkbp10), 2'-5'-oligoadneylate synthase-like protein 2 (Oasl2), interferon
induced
protein with tetratricopeptide repeats (Ifit) 1 and Ifit2. Thbs2 and Sdc2
were
significantly correlated with extracellular matrix-receptor interactions. The
results suggest that Thbs2, Sdc2, Fkbp10, Oasl2, Ifit1 and Ifit2 may serve
important
roles during the fracture healing process in osteoporosis. In addition, this
is the
first study to demonstrate that Sdc2, Fkbp10, Oasl2, Ifit1 and Ifit2 may be
associated with osteoporotic fracture healing.
FAU - Gao, Feng
AU - Gao F
AD - Department of Orthopedics, The Second Hospital of Jilin University,
Changchun, Jilin
130041, P.R. China.
FAU - Xu, Feng
AU - Xu F
AD - Department of Spine Surgery, The First Hospital of Jilin University,
Changchun,
Jilin 130021, P.R. China.
FAU - Wu, Dankai
AU - Wu D
Changchun, Jilin
130041, P.R. China.
FAU - Cheng, Jieping
AU - Cheng J
Changchun, Jilin
130041, P.R. China.
FAU - Xia, Peng
AU - Xia P
Changchun, Jilin
130041, P.R. China.
LA - eng
DEP - 20170503
TA - Mol Med Rep
JT - Molecular medicine reports
JID - 101475259
RN - 0 (Kremen protein, mouse)
RN - 0 (Low Density Lipoprotein Receptor-Related Protein-5)
RN - 0 (Lrp5 protein, mouse)
RN - 0 (Membrane Proteins)
RN - 0 (collagen type I, alpha 1 chain)
SB - IM
MH - Animals
MH - Collagen Type I/genetics
MH - Computational Biology/methods
MH - Databases, Nucleic Acid
MH - Fracture Healing/*genetics
MH - Fractures, Bone/*etiology
MH - *Gene Expression
MH - Gene Ontology
MH - Gene Regulatory Networks
MH - Low Density Lipoprotein Receptor-Related Protein-5/*deficiency/metabolism
MH - Membrane Proteins/*genetics/metabolism
MH - Mice
MH - Mice, Knockout
MH - Osteoporosis/*complications/*genetics
MH - Protein Interaction Mapping
MH - Protein Interaction Maps
PMC - PMC5436207
EDAT- 2017/05/11 06:00
MHDA- 2018/03/06 06:00
CRDT- 2017/05/11 06:00
PHST- 2016/01/20 00:00 [received]
PHST- 2017/01/30 00:00 [accepted]
PHST- 2017/05/11 06:00 [pubmed]
PHST- 2018/03/06 06:00 [medline]
PHST- 2017/05/11 06:00 [entrez]
AID - mmr-15-06-3969 [pii]
AID - 10.3892/mmr.2017.6544 [doi]
PST - ppublish
SO - Mol Med Rep. 2017 Jun;15(6):3969-3976. doi: 10.3892/mmr.2017.6544. Epub 2017
May 3.
PMID- 23289495
OWN - NLM
STAT- MEDLINE
DCOM- 20141107
LR - 20130722
VI - 22
IP - 5
DP - 2013 Jul
TI - Implant-supported fixed dental prostheses with CAD/CAM-fabricated porcelain
crown
and zirconia-based framework.
PG - 402-7
LID - 10.1111/jopr.12001 [doi]
AB - Recently, fixed dental prostheses (FDPs) with a hybrid structure of CAD/CAM
porcelain crowns adhered to a CAD/CAM zirconia framework (PAZ) have been
developed.
The aim of this report was to describe the clinical application of a newly
developed
implant-supported FDP fabrication system, which uses PAZ, and to evaluate the
outcome after a maximum application period of 36 months. Implants were placed

in
three patients with edentulous areas in either the maxilla or mandible. After
the
implant fixtures had successfully integrated with bone, gold-platinum alloy
or
zirconia custom abutments were first fabricated. Zirconia framework wax-up
was
performed on the custom abutments, and the CAD/CAM zirconia framework was
prepared
using the CAD/CAM system. Next, wax-up was performed on working models for
porcelain
crown fabrication, and CAD/CAM porcelain crowns were fabricated. The CAD/CAM
zirconia frameworks and CAD/CAM porcelain crowns were bonded using adhesive
resin
cement, and the PAZ was cemented. Cementation of the implant superstructure
improved
the esthetics and masticatory efficiency in all patients. No undesirable
outcomes,
such as superstructure chipping, stomatognathic dysfunction, or periimplant
bone
resorption, were observed in any of the patients. PAZ may be a potential
solution
for ceramic-related clinical problems such as chipping and fracture and
associated
complicated repair procedures in implant-supported FDPs.
FAU - Takaba, Masayuki
AU - Takaba M
AD - Department of Prosthodontics, Showa University School of Dentistry, Tokyo,
Japan.
mtakaba@dent.showa-u.ac.jp
FAU - Tanaka, Shinpei
AU - Tanaka S
FAU - Ishiura, Yuichi
AU - Ishiura Y
FAU - Baba, Kazuyoshi
AU - Baba K
LA - eng
PT - Case Reports
DEP - 20130104
PL - United States
TA - J Prosthodont
Prosthodontists
JID - 9301275
RN - 0 (Dental Implants)
RN - 0 (Gold Alloys)
RN - 49DFR088MY (Platinum)
RN - C6V6S92N3C (Zirconium)
RN - S38N85C5G0 (zirconium oxide)
SB - D
MH - Aged
MH - Cementation/methods
MH - *Computer-Aided Design
MH - *Crowns
MH - Dental Implants
MH - Dental Porcelain/*chemistry
MH - *Dental Prosthesis Design
MH - *Dental Prosthesis, Implant-Supported
MH - Denture Design
MH - Denture, Complete
MH - Denture, Partial, Fixed
MH - Esthetics, Dental
MH - Female
MH - Gold Alloys/chemistry
MH - Humans
MH - Male
MH - Mastication/physiology
MH - Middle Aged
MH - Osseointegration/physiology
MH - Platinum/chemistry
MH - Resin Cements/chemistry
MH - Zirconium/*chemistry
OTO - NOTNLM
OT - CAD/CAM
OT - fixed dental prostheses
OT - porcelain crown
OT - zirconia framework
EDAT- 2013/01/08 06:00
MHDA- 2014/11/08 06:00
CRDT- 2013/01/08 06:00
PHST- 2012/10/20 00:00 [accepted]
PHST- 2013/01/08 06:00 [entrez]
PHST- 2013/01/08 06:00 [pubmed]
PHST- 2014/11/08 06:00 [medline]
AID - 10.1111/jopr.12001 [doi]
PST - ppublish
SO - J Prosthodont. 2013 Jul;22(5):402-7. doi: 10.1111/jopr.12001. Epub 2013 Jan
4.
PMID- 26926190
OWN - NLM
STAT- MEDLINE
DCOM- 20160706
LR - 20181202
IS - 1002-0098 (Print)
IS - 1002-0098 (Linking)
VI - 51
IP - 2
DP - 2016 Feb
TI - [Four year's clinical evaluation of glass fiber reinforced resin-bonded fixed
partial denture as a periodontal splint to replace lost anterior teeth].

PG - 76-80
LID - 10.3760/cma.j.issn.1002-0098.2016.02.003 [doi]
AB - OBJECTIVE: To evaluate the clinical performance of glass fiber reinforced
resin-bonded fixed partial denture(GFR-RBFPD) as a periodontal splint on
abutment
teeth with reduced periodontal support. METHODS: Thirty fixed-fixed GFR-RBFPD
were
delivered to restore anterior partial edentulous dentitions. The adjacent
abutment
teeth had severely reduced periodontal support and were not indicated for
full crown
retained FPD. The success rate and functional survival rate had been recorded
and
the periodontal condition had been evaluated for 4 years. The results were
statistically analyzed with single factor variance analysis and chi square
test(α=0.05). RESULTS: In the first, second, and third year following
restoration,
one connector fractured in each year and they were repaired with adhesive
resin and
the pontics were kept in place for function. In the third and fourth years
after
restoration, there was one pontic displacement with fracture of connectors.
The
total survive rate was 83%(25/30) and the functional survival rate was 93%
(28/30)
at the end of the fourth year. The main reason of failure was fracture of
connector.
About 22%(13/60) of the adjacent teeth showed marginal bone height decrease,
while
the other 78% (47/60) increased with statistic significant difference from
one year
after the restoration to the end of the observation term(P<0.05). The
periodontal
condition of the adjacent teeth was improved after the restoration.
CONCLUSIONS: The
four years clinical evaluation indicates that the GFR-RBFPD may be used as
fixed
prostheses to replace lost one to three anterior teeth with reduced
periodontal
support of abutment teeth.
FAU - Xiao, Zunsheng
AU - Xiao Z
AD - Department of Prosthodontics, Peking University School and Hospital of
Stomatology,
Beijing 100081, China.
FAU - Jiang, Ting
AU - Jiang T
Stomatology,
FAU - Fang, Xiaoqian
AU - Fang X
Stomatology,
FAU - Lyu, Pin
AU - Lyu P
Stomatology,
FAU - Li, Jian
AU - Li J
Stomatology,
FAU - Jia, Lu
AU - Jia L
AD - Denture Processing Center, Peking University School and Hospital of
Stomatology,
LA - chi
PL - China
TA - Zhonghua Kou Qiang Yi Xue Za Zhi
JT - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese
journal
of stomatology
JID - 8711066
RN - 0 (fiberglass)
SB - D
SB - IM
MH - Composite Resins
MH - Dental Abutments
MH - Dental Restoration Failure/*statistics & numerical data
MH - Denture Design
MH - Denture Retention
MH - Denture, Partial, Fixed
MH - *Denture, Partial, Fixed, Resin-Bonded
MH - *Glass
MH - Humans
MH - *Incisor
MH - *Periodontal Splints
MH - Time Factors
EDAT- 2016/03/02 06:00
MHDA- 2016/07/07 06:00
CRDT- 2016/03/02 06:00
PHST- 2016/03/02 06:00 [entrez]
PHST- 2016/03/02 06:00 [pubmed]
PHST- 2016/07/07 06:00 [medline]
AID - 10.3760/cma.j.issn.1002-0098.2016.02.003 [doi]
PST - ppublish
SO - Zhonghua Kou Qiang Yi Xue Za Zhi. 2016 Feb;51(2):76-80. doi:
10.3760/cma.j.issn.1002-0098.2016.02.003.
PMID- 22654119
OWN - NLM
STAT- MEDLINE
DCOM- 20121011
LR - 20210205
IS - 0021-9258 (Print)
IS - 0021-9258 (Linking)
VI - 287
IP - 31
DP - 2012 Jul 27
TI - Optimal intensity shock wave promotes the adhesion and migration of rat
osteoblasts
via integrin β1-mediated expression of phosphorylated focal adhesion kinase.
PG - 26200-12
LID - 10.1074/jbc.M112.349811 [doi]
AB - To search for factors promoting bone fracture repair, we investigated the
effects of
extracorporeal shock wave (ESW) on the adhesion, spreading, and migration of
osteoblasts and its specific underlying cellular mechanisms. After a single
period
of stimulation by 10 kV (500 impulses) of shock wave (SW), the adhesion rate
was
increased as compared with the vehicle control. The data from both wound
healing and
transwell tests confirmed an acceleration in the migration of osteoblasts by
SW
treatment. RT-PCR, flow cytometry, and Western blotting showed that SW
rapidly
increased the surface expression of α5 and β1 subunit integrins, indicating
that
integrin β1 acted as an early signal for ESW-induced osteoblast adhesion and
migration. It has also been found that a significant elevation occurred in
the
expression of phosphorylated β-catenin and focal adhesion kinase (FAK) at the
site
of tyrosine 397 in response to SW stimulation after the increasing expression
of the
integrin β1 molecule. When siRNAs of integrin α5 and β1 subunit were added,
the
level of FAK phosphorylation elevated by SW declined. Interestingly, the
adhesion
and migration of osteoblasts were decreased when these siRNA reagents as well
as the
ERK1/2 signaling pathway inhibitors, U0126 and PD98059, were present. Further
studies demonstrated that U0126 could inhibit the downstream integrin-

dependent
signaling pathways, such as the FAK signaling pathway, whereas it had no
influence
on the synthesis of integrin β1 molecule. In conclusion, these data suggest
that ESW
promotes the adhesion and migration of osteoblasts via integrin β1-mediated
expression of phosphorylated FAK at the Tyr-397 site; in addition, ERK1/2 are
also
important for osteoblast adhesion, spreading, migration, and integrin
expression.
FAU - Xu, Jian-kun
AU - Xu JK
AD - Department of Orthopedics, First Affiliated Hospital, Shantou University
Medical
College, 57 Changping Road, Shantou, Guangdong 515041, China.
FAU - Chen, Hong-jiang
AU - Chen HJ
FAU - Li, Xue-dong
AU - Li XD
FAU - Huang, Zhong-lian
AU - Huang ZL
FAU - Xu, Huan
AU - Xu H
FAU - Yang, Hai-long
AU - Yang HL
FAU - Hu, Jun
AU - Hu J
LA - eng
DEP - 20120531
TA - J Biol Chem
JT - The Journal of biological chemistry
JID - 2985121R
RN - 0 (Integrin alpha5)
RN - 0 (Integrin beta1)
RN - 0 (beta Catenin)
SB - IM
MH - Animals
MH - Cell Adhesion/*radiation effects
MH - Cell Movement/*radiation effects
MH - Female
MH - Focal Adhesion Protein-Tyrosine Kinases/genetics/*metabolism
MH - *High-Energy Shock Waves
MH - Integrin alpha5/genetics/metabolism
MH - Integrin beta1/genetics/*metabolism
MH - MAP Kinase Signaling System
MH - Male
MH - Osteoblasts/metabolism/*physiology/radiation effects
MH - Primary Cell Culture
MH - Protein Processing, Post-Translational/radiation effects
MH - Rats
MH - Skull/cytology
MH - Wnt Signaling Pathway
MH - beta Catenin/metabolism
PMC - PMC3406705
EDAT- 2012/06/02 06:00
MHDA- 2012/10/12 06:00
CRDT- 2012/06/02 06:00
PHST- 2012/06/02 06:00 [entrez]
PHST- 2012/06/02 06:00 [pubmed]
PHST- 2012/10/12 06:00 [medline]
AID - S0021-9258(20)73632-X [pii]
AID - M112.349811 [pii]
AID - 10.1074/jbc.M112.349811 [doi]
PST - ppublish
SO - J Biol Chem. 2012 Jul 27;287(31):26200-12. doi: 10.1074/jbc.M112.349811. Epub
2012
May 31.
PMID- 26460819
OWN - NLM
STAT- MEDLINE
DCOM- 20161005
LR - 20161230
IS - 1944-8244 (Linking)
VI - 7
IP - 44
DP - 2015 Nov 11
TI - Cellulose Nanocrystals--Bioactive Glass Hybrid Coating as Bone Substitutes by
Electrophoretic Co-deposition: In Situ Control of Mineralization of Bioactive

Glass
and Enhancement of Osteoblastic Performance.
PG - 24715-25
LID - 10.1021/acsami.5b07294 [doi]
AB - Surface functionalization of orthopedic implants is being intensively
investigated
to strengthen bone-to-implant contact and accelerate bone healing process. A
hybrid
coating, consisting of 45S5 bioactive glass (BG) individually wrapped and
interconnected with fibrous cellulose nanocrystals (CNCs), is deposited on
316L
stainless steel from aqueous suspension by a one-step electrophoretic
deposition
(EPD) process. Apart from the codeposition mechanism elucidated by means of
zeta-potential and scanning electron microscopy measurements, in vitro
characterization of the deposited CNCs-BG coating in simulated body fluid
reveals an
extremely rapid mineralization of BG particles on the coating (e.g., the
formation
of hydroxyapatite crystals layer after 0.5 day). A series of comparative
trials and
characterization methods were carried out to comprehensively understand the
mineralization process of BG interacting with CNCs. Furthermore, key factors
for
satisfying the applicability of an implant coating such as coating
composition,
surface topography, and adhesion strength were quantitatively investigated as
a
function of mineralization time. Cell culture studies (using MC3T3-E1)
indicate that
the presence of CNCs-BG coating substantially accelerated cell attachment,
spreading, proliferation, differentiation, and mineralization of
extracellular
matrix. This study has confirmed the capability of CNCs to enhance and
regulate the
bioactivity of BG particles, leading to mineralized CNCs-BG hybrids for
improved
bone implant coatings.
FAU - Chen, Qiang
AU - Chen Q
AD - Institute of Biomaterials, Department of Materials Science and Engineering,
University of Erlangen-Nuremberg , Cauerstrasse 6, 91058 Erlangen, Germany.
FAU - Garcia, Rosalina Pérez
AU - Garcia RP
AD - CIDETEC, Parque Tecnológico de Miramón , Paseo Miramón 196, 20009 San
Sebastian,
Spain.
FAU - Munoz, Josemari
AU - Munoz J
AD - CIDETEC, Parque Tecnológico de Miramón , Paseo Miramón 196, 20009 San
Sebastian,
Spain.
FAU - Pérez de Larraya, Uxua
AU - Pérez de Larraya U
AD - CEMITEC, Materials Department, Polígono Mocholí , Plaza Cein 4, 31110 Noain,
Navarra, Spain.
FAU - Garmendia, Nere
AU - Garmendia N
AD - CEMITEC, Materials Department, Polígono Mocholí , Plaza Cein 4, 31110 Noain,
Navarra, Spain.
FAU - Yao, Qingqing
AU - Yao Q
AD - Institute of Advanced Materials for Nano-Bio Applications, School of
Ophthalmology &
Optometry, Wenzhou Medical University , Wenzhou, 270 Xueyuan Xi Road,
Zhejiang
325027, China.
FAU - Boccaccini, Aldo R
AU - Boccaccini AR
AD - Institute of Biomaterials, Department of Materials Science and Engineering,
University of Erlangen-Nuremberg , Cauerstrasse 6, 91058 Erlangen, Germany.
LA - eng
DEP - 20151027
PL - United States
JID - 101504991
RN - 0 (Ions)
RN - 0 (Solvents)
RN - 0 (bioactive glass 45S5)
RN - 9004-34-6 (Cellulose)
SB - IM
MH - 3T3 Cells
MH - Animals
MH - Body Fluids
MH - Cell Adhesion
MH - Cell Survival
MH - Cellulose/*chemistry
MH - Ceramics/*chemistry
MH - Electrophoresis
MH - Extracellular Matrix/metabolism
MH - Glass/*chemistry
MH - Ions
MH - Mice
MH - Nanoparticles/*chemistry
MH - Orthopedics
MH - Osteoblasts/*metabolism
MH - Solvents/chemistry
MH - Temperature
MH - X-Ray Diffraction
OTO - NOTNLM
OT - electrophoresis
OT - guided mineralization
OT - hybrid materials
OT - nanocellulose
EDAT- 2015/10/16 06:00
MHDA- 2016/10/07 06:00
CRDT- 2015/10/14 06:00
PHST- 2015/10/14 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2016/10/07 06:00 [medline]
AID - 10.1021/acsami.5b07294 [doi]
PST - ppublish
SO - ACS Appl Mater Interfaces. 2015 Nov 11;7(44):24715-25. doi:
10.1021/acsami.5b07294.
Epub 2015 Oct 27.
PMID- 22248526
OWN - NLM
STAT- MEDLINE
DCOM- 20120622
LR - 20131121
IS - 1742-7061 (Linking)
VI - 8
IP - 4
DP - 2012 Apr
TI - Effect of Si and Fe doping on calcium phosphate glass fibre reinforced
polycaprolactone bone analogous composites.
PG - 1616-26
LID - 10.1016/j.actbio.2011.12.030 [doi]
AB - Reinforcing biodegradable polymers with phosphate-based glass fibres (PGF) is
of
interest for bone repair and regeneration. In addition to increasing the
mechanical
properties, PGF can also release bioinorganics, as they are water soluble, a
property that may be controllably translated into a fully degradable
composite.
Herein, the effect of Si and Fe on the solubility of calcium-containing
phosphate-based glasses (PG) in the system
(50P(2)O(5)-40CaO-(10-x)SiO(2)-xFe(2)O(3), where x=0, 5 and 10 mol.%) were
investigated. On replacing SiO(2) with Fe(2)O(3), there was an increase in
the glass
transition temperature and density of the PG, suggesting greater crosslinking
of the
phosphate chains. This significantly reduced the dissolution rates of
degradation
and ion release. Two PG formulations, 50P(2)O(5)-40CaO-10Fe(2)O(3) (Fe10) and
50P(2)O(5)-40CaO-5Fe(2)O(3)-5SiO(2) (Fe5Si5), were melt drawn into fibres and
randomly incorporated into polycaprolactone (PCL). Initially, the flexural

strength
and modulus significantly increased with PGF incorporation. In deionized
water,
PCL-Fe(5)Si(5) displayed a significantly greater weight loss and ion release
compared with PCL-Fe10. In simulated body fluid, brushite was formed only on
the
surface of PCL-Fe(5)Si(5). Dynamic mechanical analysis in phosphate buffered
saline
(PBS) at 37°C revealed that the PCL-Fe10 storage modulus (E') was unchanged
up to
day 7, whereas the onset of PCL-Fe(5)Si(5)E' decrease occurred at day 4. At
longer-term ageing in PBS, PCL-Fe(5)Si(5) flexural strength and modulus
decreased
significantly. MC3T3-E1 preosteoblasts seeded onto PCL-PGF grew up to day 7
in
culture. PGF can be used to control the properties of biodegradable
composites for
potential application as bone fracture fixation devices.
CI - Copyright Â© 2012 Acta Materialia Inc. All rights reserved.
FAU - Mohammadi, M Shah
AU - Mohammadi MS
AD - Department of Mining and Materials Engineering, McGill University, Montreal,
QC,
Canada H3A 2B2.
FAU - Ahmed, I
AU - Ahmed I
FAU - Muja, N
AU - Muja N
FAU - Almeida, S
AU - Almeida S
FAU - Rudd, C D
AU - Rudd CD
FAU - Bureau, M N
AU - Bureau MN
FAU - Nazhat, S N
AU - Nazhat SN
LA - eng
DEP - 20120110
PL - England
TA - Acta Biomater
JID - 101233144
RN - 0 (Ions)
RN - 0 (Polyesters)
RN - 059QF0KO0R (Water)
RN - E1UOL152H7 (Iron)
RN - Z4152N8IUI (Silicon)
SB - IM
MH - Animals
MH - Body Fluids/chemistry
MH - Bone Substitutes/*pharmacology
MH - Differential Thermal Analysis
MH - Hydrogen-Ion Concentration/drug effects
MH - Ions
MH - Iron/*pharmacology
MH - Mice
MH - Polyesters/*pharmacology
MH - Silicon/*pharmacology
MH - Temperature
MH - Time Factors
MH - Water/chemistry
EDAT- 2012/01/18 06:00
MHDA- 2012/06/23 06:00
CRDT- 2012/01/18 06:00
PHST- 2011/08/30 00:00 [received]
PHST- 2011/12/19 00:00 [revised]
PHST- 2011/12/22 00:00 [accepted]
PHST- 2012/01/18 06:00 [entrez]
PHST- 2012/01/18 06:00 [pubmed]
PHST- 2012/06/23 06:00 [medline]
AID - S1742-7061(11)00566-6 [pii]
AID - 10.1016/j.actbio.2011.12.030 [doi]
PST - ppublish
SO - Acta Biomater. 2012 Apr;8(4):1616-26. doi: 10.1016/j.actbio.2011.12.030. Epub
2012
Jan 10.
PMID- 26415835
OWN - NLM
STAT- MEDLINE
DCOM- 20160808
LR - 20191210
IS - 2045-2322 (Linking)
VI - 5
DP - 2015 Sep 29
TI - Synergy of multi-scale toughening and protective mechanisms at hierarchical
branch-stem interfaces.
PG - 14522
LID - 10.1038/srep14522 [doi]
LID - 14522
AB - Biological materials possess a variety of artful interfaces whose size and
properties are adapted to their hierarchical levels and functional
requirements.
Bone, nacre, and wood exhibit an impressive fracture resistance based mainly
on
small crystallite size, interface organic adhesives and hierarchical
microstructure.
Currently, little is known about mechanical concepts in macroscopic
biological
interfaces like the branch-stem junction with estimated 10(14) instances on
earth
and sizes up to few meters. Here we demonstrate that the crack growth in the
upper
region of the branch-stem interface of conifer trees proceeds along a narrow
predefined region of transversally loaded tracheids, denoted as sacrificial
tissue,
which fail upon critical bending moments on the branch. The specific
arrangement of
the tracheids allows disconnecting the overloaded branch from the stem in a
controlled way by maintaining the stem integrity. The interface
microstructure based
on the sharply adjusted cell orientation and cell helical angle secures a
zig-zag
crack propagation path, mechanical interlock closing after the bending moment
is
removed, crack gap bridging and self-repairing by resin deposition. The
multi-scale
synergetic concepts allows for a controllable crack growth between stiff stem
and
flexible branch, as well as mechanical tree integrity, intact physiological
functions and recovery after the cracking.
FAU - Müller, Ulrich
AU - Müller U
AD - Institute of Wood Technology and Renewable Materials, University of Natural
Resources and Life Science, Vienna, Austria.
FAU - Gindl-Altmutter, Wolfgang
AU - Gindl-Altmutter W
FAU - Konnerth, Johannes
AU - Konnerth J
FAU - Maier, Günther A
AU - Maier GA
AD - Materials Center Leoben, Leoben, Austria.
FAU - Keckes, Jozef
AU - Keckes J
AD - Department of Materials Physics, Montanuniversität Leoben, Leoben, Austria.
LA - eng
DEP - 20150929
TA - Sci Rep
JID - 101563288
SB - IM
MH - Hardness Tests
MH - Plant Shoots/anatomy & histology/*physiology
MH - Plant Stems/anatomy & histology/*physiology
MH - Tensile Strength
MH - Tracheophyta/anatomy & histology/*physiology
MH - Trees/anatomy & histology/*physiology
PMC - PMC4586606
EDAT- 2015/09/30 06:00
MHDA- 2016/08/09 06:00
CRDT- 2015/09/30 06:00
PHST- 2015/02/25 00:00 [received]
PHST- 2015/08/28 00:00 [accepted]
PHST- 2015/09/30 06:00 [entrez]
PHST- 2015/09/30 06:00 [pubmed]
PHST- 2016/08/09 06:00 [medline]
AID - 10.1038/srep14522 [doi]
PST - epublish
SO - Sci Rep. 2015 Sep 29;5:14522. doi: 10.1038/srep14522.
PMID- 22082621
OWN - NLM
STAT- MEDLINE
DCOM- 20120402
LR - 20201209
IS - 0142-9612 (Linking)
VI - 33
IP - 5
DP - 2012 Feb
TI - Hydrophobic polycationic coatings that inhibit biofilms and support bone
healing
during infection.
PG - 1245-54
AB - Adhesion of microorganisms to biomaterials with subsequent formation of
biofilms on
such foreign bodies as orthopedic trauma hardware is a critical factor in
implant-associated infections; once a biofilm has been established, its
microorganisms become recalcitrant to the host's immune surveillance and
markedly
resistant to drugs. We have previously reported that painting with the
hydrophobic
polycation N,N-dodecyl,methyl-PEI (PEI = polyethylenimine) renders solid
surfaces
bactericidal in vitro. Herein we observe that N,N-dodecyl,methyl-PEI-
derivatized
titanium and stainless steel surfaces resist biofilm formation by
Staphylococcus
aureus compared to the untreated ones. Using imaging, microbiology-,
histopathology-, and scanning electron microscopy (SEM) experiments in a
clinically
relevant large-animal (sheep) trauma model, we subsequently demonstrate in
vivo that
orthopedic fracture hardware painted with N,N-dodecyl,methyl-PEI not only
prevents
implant colonization with biofilm but also promotes bone healing.
Functionalizing
orthopedic hardware with hydrophobic polycations thus holds promise in
supporting
bone healing in the presence of infection in veterinary and human orthopedic
patients.
FAU - Schaer, Thomas P
AU - Schaer TP
AD - Department of Clinical Studies, New Bolton Center, University of Pennsylvania
School
of Veterinary Medicine, Kennett Square, PA 19348, USA. tpschaer@vet.upenn.edu
FAU - Stewart, Suzanne
AU - Stewart S
FAU - Hsu, Bryan B
AU - Hsu BB
FAU - Klibanov, Alexander M
AU - Klibanov AM
LA - eng
DEP - 20111113
PL - Netherlands
TA - Biomaterials
JT - Biomaterials
JID - 8100316
RN - 0 (Polyamines)
RN - 0 (Polyelectrolytes)
RN - 0 (polycations)
RN - 9002-98-6 (Polyethyleneimine)
SB - IM
MH - Animals
MH - Biofilms/*drug effects
MH - Bone and Bones/diagnostic imaging/*drug effects/pathology/surgery
MH - Colony Count, Microbial
MH - Humans
MH - Hydrophobic and Hydrophilic Interactions/*drug effects
MH - Osteotomy
MH - Polyamines/*pharmacology
MH - Polyelectrolytes
MH - Polyethyleneimine/pharmacology
MH - Radiography
MH - Sheep
MH - Staphylococcal Infections/microbiology/*pathology
MH - Staphylococcus aureus/drug effects/growth & development/physiology
EDAT- 2011/11/16 06:00
MHDA- 2012/04/03 06:00
CRDT- 2011/11/16 06:00
PHST- 2011/07/11 00:00 [received]
PHST- 2011/10/01 00:00 [accepted]
PHST- 2011/11/16 06:00 [entrez]
PHST- 2011/11/16 06:00 [pubmed]
PHST- 2012/04/03 06:00 [medline]
AID - S0142-9612(11)01242-7 [pii]
PST - ppublish
SO - Biomaterials. 2012 Feb;33(5):1245-54. doi:
10.1016/j.biomaterials.2011.10.038. Epub
2011 Nov 13.
PMID- 26179911
OWN - NLM
STAT- MEDLINE
DCOM- 20160316
LR - 20150716
IS - 1748-3182 (Linking)
VI - 10
IP - 4
DP - 2015 Jul 16
TI - Experimental and numerical measurements of adhesion energies between PHEMA
and
PGLYMA with hydroxyapatite crystal.
PG - 046011
LID - 10.1088/1748-3190/10/4/046011 [doi]
AB - Synthetic orthopaedic materials consisting of a single bioinert polymeric
material
do not meet the complex biological and physical requirements of scaffold-
guided bone
tissue repair and regeneration. Of particular interest is the design of
biocompatible hydrogel-hydroxyapatite composite bone substitutes with
outstanding
interfacial adhesion that would warranty the ability for the composite to
withstand
functional loadings without exhibiting brittle fractures during the dynamic
guided
tissue regeneration. For this purpose, the hydroxylated side chain of
chemically
cross-linked poly (2-hydroxyethyl methacrylate) (pHEMA) is substitute with a
carboxylated side chain to make poly (glycerol methacrylate) (pGLYMA). Here,
we
carry out atomistic simulations and atomic force microscopy to predict and
experimentally determine the interfacial adhesion energies of pHEMA and
pGLYMA with
the surface of single-crystalline hydroxyapatite (HA) whiskers. Both
experimental
and numerical results showed that pGLYMA has stronger adhesion forces with HA
and
may be used for preparing a high-affinity polymer-HA composite. The high
adhesive
interactions between pGLYMA and HA were found to be due to strong
electrostatic
energies.
FAU - Youssefian, Sina
AU - Youssefian S
AD - Department of Mechanical Engineering, Worcester Polytechnic Institute, 100
Institute
Rd, Worcester, MA 01609, USA.
FAU - Liu, Pingsheng
AU - Liu P
FAU - Askarinejad, Sina
AU - Askarinejad S
FAU - Shalchy, Faezeh
AU - Shalchy F
FAU - Song, Jie
AU - Song J
FAU - Rahbar, Nima
AU - Rahbar N
LA - eng
DEP - 20150716
PL - England
TA - Bioinspir Biomim
JT - Bioinspiration & biomimetics
JID - 101292902
RN - 0 (poly(glycerol dimethacrylate))
RN - 25249-16-5 (Polyhydroxyethyl Methacrylate)
SB - IM
MH - Adhesiveness
MH - Bone Substitutes/*chemical synthesis
MH - Computer Simulation
MH - Crystallization
MH - Energy Transfer
MH - Methacrylates/*chemistry
MH - *Models, Chemical
MH - Polyhydroxyethyl Methacrylate/*chemistry
EDAT- 2015/07/17 06:00
MHDA- 2016/03/17 06:00
CRDT- 2015/07/17 06:00
PHST- 2015/07/17 06:00 [entrez]
PHST- 2015/07/17 06:00 [pubmed]
PHST- 2016/03/17 06:00 [medline]
AID - 10.1088/1748-3190/10/4/046011 [doi]
PST - epublish
SO - Bioinspir Biomim. 2015 Jul 16;10(4):046011. doi: 10.1088/1748-
3190/10/4/046011.
PMID- 25158903
OWN - NLM
STAT- MEDLINE
DCOM- 20150812
LR - 20141202
IS - 0894-1130 (Linking)
VI - 27
IP - 4
DP - 2014 Oct-Dec
TI - Swing traction versus no-traction for complex intra-articular proximal
inter-phalangeal fractures.
PG - 309-15; quiz 316
LID - S0894-1130(14)00079-9 [pii]
LID - 10.1016/j.jht.2014.07.003 [doi]
AB - INTRODUCTION: Traction orthoses are thought to optimize recovery from
intra-articular finger fractures by restoring joint space and allowing early
motion.
Evidence to date has, however, consisted only of case series. PURPOSE OF THE
STUDY:
To compare swing traction versus no-traction management of complex fractures
of
proximal inter-phalangeal (PIP) finger joints. We hypothesized that there is
no
long-term (i.e. >12 month) difference between swing traction and no-traction
(with
or without surgical fixation) in terms of motion, pain, function, patient
satisfaction, or treatment cost. METHODS: Adults with a history of complex
PIP
fractures affecting ≥30% of articular surface injury were identified from
database
searches at three public hospitals and a private clinic and invited to
participate.
X-rays taken at the time of injury were graded by two blinded assessors, and
participants attended a clinic for measurement of range of motion (ROM) and
self-reported function, pain, and satisfaction at least one year post injury.
Participant data were then were grouped by treatment provided. One group (N =
17)
was treated with swing traction and the other group (N = 14) had no-traction.
The
primary outcome was combined motion of the PIP and distal inter-phalangeal
(DIP)
joints, expressed as both total active motion and Strickland score. Secondary
outcomes were physical function and symptoms as measured by the Disabilities

of Arm,
Shoulder and Hand (DASH), patient satisfaction, pain, complication rates, and
cost
of treatment, based on mean resource consumption per group. RESULTS: Patients
treated with swing traction had greater finger motion than those in the no-
traction
group, which was statistically and clinically significant. There were no
differences
in patient ratings of function, pain or satisfaction. Complications, such as
swan-neck deformity, cold sensitivity, malunion, infection, or adhesions
occurred in
over half of both groups of participants. During the treatment phase, the
swing
traction group attended hand therapy an average of 13.3 times, and the no-
traction
group attended 11.7 times. Average costs for swing traction were less than
for
surgical fixation with no-traction. DISCUSSION: The significantly different
range of
motion found in our study did not translate to better DASH scores. The DASH
is
designed to measure global upper limb physical functioning and symptoms, but
lacks
sensitivity in populations with finger injuries. CONCLUSIONS: Patients
treated with
the swing traction protocol had greater range of motion in the finger,
however this
did not translate to improved patient ratings of function, pain or
satisfaction. A
basic cost comparison indicated that swing traction may be less expensive
than other
forms of surgical repair. LEVEL OF EVIDENCE: 3.
CI - Crown Copyright © 2014. Published by Elsevier Inc. All rights reserved.
FAU - O'Brien, Lisa J
AU - O'Brien LJ
AD - Department of Occupational Therapy, The Alfred, Melbourne, Victoria,
Australia;
Department of Occupational Therapy, Monash University, Melbourne, Victoria,
Australia. Electronic address: lisa.obrien@monash.edu.
FAU - Simm, Andrew T
AU - Simm AT
AD - Plastic and Reconstructive Unit, Melbourne Health, Melbourne, Victoria,
Australia.
FAU - Loh, Ian W H
AU - Loh IW
AD - Department of Plastic Surgery, The Alfred, Melbourne, Victoria, Australia.
FAU - Griffiths, Kim M
AU - Griffiths KM
AD - Department of Occupational Therapy, Monash Health, Melbourne, Victoria,
Australia.
LA - eng
PT - Observational Study
DEP - 20140723
PL - United States
TA - J Hand Ther
JT - Journal of hand therapy : official journal of the American Society of Hand
Therapists
JID - 8806591
SB - IM
MH - Adult
MH - Aged
MH - Cohort Studies
MH - Female
MH - Finger Injuries/*rehabilitation
MH - Fractures, Bone/*rehabilitation
MH - Humans
MH - Injury Severity Score
MH - Intra-Articular Fractures/*rehabilitation
MH - Male
MH - Middle Aged
MH - *Orthotic Devices
MH - Patient Satisfaction/statistics & numerical data
MH - Range of Motion, Articular/*physiology
MH - Reference Values
MH - Risk Assessment
MH - Time Factors
MH - Traction/instrumentation/*methods
MH - Young Adult
OTO - NOTNLM
OT - Dislocation
OT - Finger
OT - Fracture
OT - Orthosis
OT - Traction
EDAT- 2014/08/28 06:00
MHDA- 2015/08/13 06:00
CRDT- 2014/08/28 06:00
PHST- 2014/03/24 00:00 [received]
PHST- 2014/07/15 00:00 [revised]
PHST- 2014/07/15 00:00 [accepted]
PHST- 2014/08/28 06:00 [entrez]
PHST- 2014/08/28 06:00 [pubmed]
PHST- 2015/08/13 06:00 [medline]
AID - S0894-1130(14)00079-9 [pii]
AID - 10.1016/j.jht.2014.07.003 [doi]
PST - ppublish
SO - J Hand Ther. 2014 Oct-Dec;27(4):309-15; quiz 316. doi:
10.1016/j.jht.2014.07.003.
Epub 2014 Jul 23.
PMID- 26723294
OWN - NLM
STAT- MEDLINE
DCOM- 20160822
LR - 20191019
IS - 1878-8750 (Linking)
VI - 88
DP - 2016 Apr
TI - Posttraumatic Bilateral Epidural Hygroma of the Posterior Cranial Fossa: Case
Report
and Brief Review of Literature.
PG - 694.e1-694.e4
LID - S1878-8750(15)01766-0 [pii]
LID - 10.1016/j.wneu.2015.12.062 [doi]
AB - BACKGROUND: Posttraumatic posterior fossa epidural hygroma is a rare entity,
and a
clear management has not been established in the medical literature. We
present 1
case and review the literature relevant to this unusual entity. The mechanism
of
formation and management of posterior cranial fossa epidural hygroma are also
outlined. CASE DESCRIPTION: A 2-year-old child presented after a rooftop fall

injury
with symptoms of headache, drowsiness, vomiting, and brief loss of
consciousness.
Computed tomography scan demonstrated swelling in the left occipital region
and
epidural hygroma. After conservative management failed, surgical repair of
the dura
mater was performed. The child was discharged postoperative day 11 in stable
condition with marked improvement in occipital swelling. CONCLUSIONS: It is
imperative to consider epidural hygroma in very small children presenting
with
occipital injury. As a result of loose adhesion of dura mater and internal
cranial
lamina layers in younger pediatric patients, potential epidural space may be
easily
created secondary to injury, and small breaches in meningeal integrity near
the
cisterna magna may favor cerebrospinal fluid leak. During surgery, if
watertight
repair of a visible dural tear is performed, duro-periosteal hitching or
vacuum
drain placement may not be required.
FAU - Kumar, Jayendra
AU - Kumar J
AD - Department of Neurosurgery, Narayan Medical College and Hospital, Sasaram,
Bihar,
India.
FAU - Harsh, Viraat
AU - Harsh V
AD - Department of Neurosurgery, Rajendra Institute of Medical Sciences, Ranchi,
Jharkhand, India.
FAU - Strickland, Ben A
AU - Strickland BA
AD - Department of Neurosurgery, The Keck School of Medicine of the University of
Southern California, Los Angeles, California, USA.
FAU - Sahay, Chandra B
AU - Sahay CB
Jharkhand, India.
FAU - Kumar, Anil
AU - Kumar A
Jharkhand, India. Electronic address: dr.anilkumarranchi@gmail.com.
LA - eng
PT - Case Reports
PT - Review
DEP - 20151224
PL - United States
TA - World Neurosurg
JT - World neurosurgery
JID - 101528275
SB - IM
MH - Brain Injuries/complications/*pathology/surgery
MH - Cranial Fossa, Posterior/injuries/pathology/*surgery
MH - Diagnosis, Differential
MH - Humans
MH - Male
MH - Neurosurgical Procedures/*methods
MH - Reconstructive Surgical Procedures/methods
MH - Subdural Effusion/*etiology/pathology/*surgery
OTO - NOTNLM
OT - Cerebrospinal fluid
OT - Epidural hygroma
OT - Occipital bone fracture
OT - Posterior cranial fossa
EDAT- 2016/01/03 06:00
MHDA- 2016/08/23 06:00
CRDT- 2016/01/03 06:00
PHST- 2015/09/28 00:00 [received]
PHST- 2015/12/14 00:00 [revised]
PHST- 2015/12/15 00:00 [accepted]
PHST- 2016/01/03 06:00 [entrez]
PHST- 2016/01/03 06:00 [pubmed]
PHST- 2016/08/23 06:00 [medline]
AID - S1878-8750(15)01766-0 [pii]
AID - 10.1016/j.wneu.2015.12.062 [doi]
PST - ppublish
SO - World Neurosurg. 2016 Apr;88:694.e1-694.e4. doi: 10.1016/j.wneu.2015.12.062.
Epub
2015 Dec 24.
PMID- 25242729
OWN - NLM
STAT- MEDLINE
DCOM- 20161111
LR - 20170103
IS - 1888-4415 (Linking)
VI - 59
IP - 1
DP - 2015 Jan-Feb
TI - [Use of adipose-derived stem cells in an experimental rotator cuff fracture
animal
model].
PG - 3-8
LID - S1888-4415(14)00168-4 [pii]
LID - 10.1016/j.recot.2014.07.008 [doi]
AB - AIM: Rotator cuff repairs have shown a high level of re-ruptures. We
hypothesized
that the use of adipose-derived stem cells (ASC) could improve the
biomechanical and
histological properties of the repair. MATERIAL AND METHODS: Controlled
experimental
study conducted on 44 BDIX rats with section and repair of the supraspinatus
tendon
and randomization to one of three groups: group A, no intervention (control);
group
B, local applications of a fibrin sealant; and group C, application of the
fibrin
sealant with 2 x 10(6) ASC. At 4 and 8 weeks a biomechanical and histological
analysis was performed. RESULTS: There were no differences in load-to-failure

at 4
and 8 weeks between groups. The load-to-failure did increase between week 4
and week
8. Histologically the tendon-to bone union showed a disorganized
fibrovascular
tissue. Group C showed a different inflammatory pattern, with less presence
of
neutrophils and more presence of plasma cells. CONCLUSION: The use of ASC
does not
improve the biomechanical or histological properties of the repair site. More
studies are needed to improve techniques that enhance the healing site of the
repair.
CI - Copyright © 2014 SECOT. Published by Elsevier Espana. All rights reserved.
FAU - Barco, R
AU - Barco R
AD - Servicio de Cirugía Ortopédica y Traumatología, IDIPAZ, Madrid, España.
Electronic
address: raulbarco@hotmail.com.
FAU - Encinas, C
AU - Encinas C
FAU - Valencia, M
AU - Valencia M
FAU - Carrascal, M T
AU - Carrascal MT
AD - Departamento de Medicina. Escuela Técnica Superior Ingeniaría Industrial,
UNED,
Madrid, España.
FAU - García-Arranz, M
AU - García-Arranz M
AD - Unidad de Terapia Celular, Instituto de Investigación Sanitaria-Fundación
Jiménez
Díaz, Madrid, España.
FAU - Antuña, S
AU - Antuña S
LA - spa
TT - Uso de células troncales derivadas de lipoaspirado en un modelo experimental
animal
de rotura de manguito rotador.
DEP - 20140918
PL - Spain
TA - Rev Esp Cir Ortop Traumatol
JT - Revista espanola de cirugia ortopedica y traumatologia
JID - 101477399
SB - IM
MH - Animals
MH - Combined Modality Therapy
MH - Rats
MH - *Rotator Cuff Injuries
MH - Subcutaneous Fat/*cytology
MH - Tendon Injuries/*therapy
OTO - NOTNLM
OT - Adipose-derived stem-cell
OT - Cell therapy
OT - Celula troncal derivada de lipoaspirado
OT - Experimental
OT - Manguito rotador
OT - Repair
OT - Reparación
OT - Supraspinatus
OT - Terapia celular
EDAT- 2014/09/23 06:00
MHDA- 2016/11/12 06:00
CRDT- 2014/09/23 06:00
PHST- 2014/05/19 00:00 [received]
PHST- 2014/07/12 00:00 [revised]
PHST- 2014/07/15 00:00 [accepted]
PHST- 2014/09/23 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2016/11/12 06:00 [medline]
AID - S1888-4415(14)00168-4 [pii]
AID - 10.1016/j.recot.2014.07.008 [doi]
PST - ppublish
SO - Rev Esp Cir Ortop Traumatol. 2015 Jan-Feb;59(1):3-8. doi:
10.1016/j.recot.2014.07.008. Epub 2014 Sep 18.
PMID- 23962074
OWN - NLM
STAT- MEDLINE
DCOM- 20140828
LR - 20181202
IS - 1547-3287 (Print)
IS - 1547-3287 (Linking)
VI - 23
IP - 1
DP - 2014 Jan 1
TI - Aqp1 enhances migration of bone marrow mesenchymal stem cells through
regulation of
FAK and β-catenin.
PG - 66-75
LID - 10.1089/scd.2013.0185 [doi]
AB - Bone marrow mesenchymal stem cells (MSCs) have the potential to migrate to
the site
of injury and regulate the repair process. Aquaporin 1 (Aqp1) is a water
channel
molecule and a regulator of endothelial cell migration. To study the role of
Apq1 in
MSC migration, we manipulated the expression of the Aqp1 gene in MSCs and
explored
its effects on MSC migration both in vitro and in vivo. Overexpression of
Aqp1
promoted MSC migration, while depletion of Aqp1 impaired MSC migration in
vitro.
When the green fluorescent protein (GFP) labeled Aqp1 overexpressing MSCs
were
systemically injected into rats with a femoral fracture, there were
significantly
more GFP-MSCs found at the fracture gap in the Aqp1-GFP-MSC-treated group
compared
to the GFP-MSC group. To elucidate the underlying mechanism, we screened
several
migration-related regulators. The results showed that β-catenin and focal
adhesion
kinase (FAK) were upregulated in the Aqp1-MSCs and downregulated in the
Aqp1-depleted MSCs, while C-X-C chemokine receptor type 4 had no change.
Furthermore, β-catenin and FAK were co-immunoprecipitated with Aqp1, and
depletion
of FAK abolished the Aqp1 effects on MSC migration. This study demonstrates
that
Aqp1 enhances MSC migration ability mainly through the FAK pathway and
partially
through the β-catenin pathway. Our finding suggests a novel function of Aqp1
in
governing MSC migration, and this may aid MSC therapeutic applications.
FAU - Meng, Fanbiao
AU - Meng F
AD - 1 Department of Orthopaedics and Traumatology, Faculty of Medicine, Prince of
Wales
Hospital, The Chinese University of Hong Kong , Shatin, Hong Kong SAR,
People's
Republic of China .
FAU - Rui, Yunfeng
AU - Rui Y
FAU - Xu, Liangliang
AU - Xu L
FAU - Wan, Chao
AU - Wan C
FAU - Jiang, Xiaohua
AU - Jiang X
FAU - Li, Gang
AU - Li G
LA - eng
DEP - 20130927
TA - Stem Cells Dev
JT - Stem cells and development
JID - 101197107
RN - 0 (Aqp1 protein, rat)
RN - 0 (Cxcr4 protein, rat)
RN - 0 (Receptors, CXCR4)
RN - 0 (beta Catenin)
RN - 146410-94-8 (Aquaporin 1)
RN - 147336-22-9 (Green Fluorescent Proteins)
RN - EC 2.7.10.2 (Focal Adhesion Kinase 1)
RN - EC 2.7.10.2 (Ptk2 protein, rat)
SB - IM
MH - Animals
MH - Aquaporin 1/biosynthesis/genetics/*metabolism
MH - Bone Marrow Cells/metabolism
MH - Bone Marrow Transplantation
MH - Bone Regeneration/genetics/*physiology
MH - Cell Movement/genetics
MH - Cell- and Tissue-Based Therapy
MH - Focal Adhesion Kinase 1/biosynthesis/*metabolism
MH - Fractures, Bone/genetics/therapy
MH - Green Fluorescent Proteins
MH - Male
MH - Mesenchymal Stem Cell Transplantation
MH - Mesenchymal Stem Cells/*metabolism
MH - Rats
MH - Receptors, CXCR4/biosynthesis
MH - Tibia/cytology/injuries
MH - beta Catenin/biosynthesis/*metabolism
PMC - PMC3870604
EDAT- 2013/08/22 06:00
MHDA- 2014/08/29 06:00
CRDT- 2013/08/22 06:00
PHST- 2013/08/22 06:00 [entrez]
PHST- 2013/08/22 06:00 [pubmed]
PHST- 2014/08/29 06:00 [medline]
AID - 10.1089/scd.2013.0185 [pii]
AID - 10.1089/scd.2013.0185 [doi]
PST - ppublish
SO - Stem Cells Dev. 2014 Jan 1;23(1):66-75. doi: 10.1089/scd.2013.0185. Epub 2013
Sep
27.
PMID- 27861393
OWN - NLM
STAT- MEDLINE
DCOM- 20170207
LR - 20210109
IS - 0025-7974 (Print)
IS - 0025-7974 (Linking)
VI - 95
IP - 46
DP - 2016 Nov
TI - Vacuum sealing drainage therapy in the presence of an external fixation
device: A
case report.
PG - e5444
LID - 10.1097/MD.0000000000005444 [doi]
LID - e5444
AB - RATIONALE: Vacuum sealing drainage (VSD) is widely utilized for treating
traumatic
wounds. PATIENT CONCERNS: It is particularly difficult and time consuming to
use in
combination with an external fixator. DIAGNOSES: This is because the hardware
or
pins used for fixation interfere with maintaining a seal, resulting in poor
adhesion
and subsequent air leakage. INTERVENTIONS: To resolve this problem, we have
devised
a new method for sealing the wound dressing, while maintaining the required
vacuum.When using this technique, a rubber strip is wrapped around each pin
in 3
circles outside the plastic drape, and then tightly tied. OUTCOMES: After
completing
this procedure, a vacuum is obtained, and any air leakage stops. We employed
this
technique to treat a cohort of patients in our department over a period of
two
years, and obtained good healing of soft tissue without air leakage, as well
as good
clinical outcomes. LESSONS: We have observed that patients treated with this
method
experienced good clinical outcomes without air leakage, and we recommend its
use in
treating cases where an external fixation device is present.
FAU - Sun, Dahui
AU - Sun D
AD - Division of Orthopedic Traumatology, The First Hospital of Jilin University
Department of Neurology, The First Hospital of Jilin University, Changchun,
China.
FAU - Ju, Weina
AU - Ju W
FAU - Wang, Tiejun
AU - Wang T
FAU - Yu, Tiecheng
AU - Yu T
FAU - Qi, Baochang
AU - Qi B
LA - eng
PT - Case Reports
TA - Medicine (Baltimore)
JT - Medicine
JID - 2985248R
RN - 9006-04-6 (Rubber)
SB - AIM
SB - IM
MH - Adult
MH - China
MH - *External Fixators
MH - Fracture Fixation/instrumentation
MH - Humans
MH - Male
MH - *Negative-Pressure Wound Therapy/instrumentation/methods
MH - Rubber/*therapeutic use
MH - Wound Healing
MH - Wounds and Injuries/*therapy
PMC - PMC5120950
COIS- The authors have no conflicts of interest to disclose.
EDAT- 2016/11/20 06:00
MHDA- 2017/02/09 06:00
CRDT- 2016/11/19 06:00
PHST- 2016/11/19 06:00 [entrez]
PHST- 2016/11/20 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
AID - 00005792-201611150-00064 [pii]
AID - 10.1097/MD.0000000000005444 [doi]
PST - ppublish
SO - Medicine (Baltimore). 2016 Nov;95(46):e5444. doi:
10.1097/MD.0000000000005444.
PMID- 23238777
OWN - NLM
STAT- MEDLINE
DCOM- 20130507
LR - 20200225
IS - 0736-0266 (Print)
IS - 0736-0266 (Linking)
VI - 31
IP - 5
DP - 2013 May
TI - Effects of local insulin delivery on subperiosteal angiogenesis and
mineralized
tissue formation during fracture healing.
PG - 783-91
LID - 10.1002/jor.22288 [doi]
AB - Local insulin delivery has been shown to improve osseous healing in diabetic
animals. The purpose of this study was to quantify the effects of local
intramedullary delivery of saline or Ultralente insulin (UL) on various
fracture
healing parameters using an in vivo non-diabetic BB Wistar rat model.
Quantitation
of local insulin levels showed a rapid release of insulin from the fractured
femora,
demonstrating complete release at 2 days. RT-PCR analysis revealed that the
expression of early osteogenic markers (Col1α2, osteopontin) was
significantly
enhanced with UL treatment when compared with saline controls (p < 0.05).
Significant differences in VEGF + cells and vascularity were evident between
the
treatment and control groups at day 7 (p < 0.05). At day 21,
histomorphometric
analysis demonstrated a significant increase in percent mineralized tissue in
the
UL-treated animals compared with controls (p < 0.05), particularly within the
subperiosteal region of the fracture callus. Mechanical testing at 4 weeks

showed
significantly greater mechanical strength for UL-treated animals (p < 0.05),
but
healing in control animals caught up at 6 weeks post-fracture. These results
suggest
that the primary osteogenic effect of UL during the early stages of fracture
healing
(1-3 weeks) is through an increase in osteogenic gene expression,
subperiosteal
angiogenesis, and mineralized tissue formation.
FAU - Paglia, David N
AU - Paglia DN
AD - Department of Orthopaedics, University of Medicine and Dentistry of New
Jersey-New
Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, NJ 07103, USA.
dnp6@njit.edu
FAU - Wey, Aaron
AU - Wey A
FAU - Breitbart, Eric A
AU - Breitbart EA
FAU - Faiwiszewski, Jonathan
AU - Faiwiszewski J
FAU - Mehta, Siddhant K
AU - Mehta SK
FAU - Al-Zube, Loay
AU - Al-Zube L
FAU - Vaidya, Swaroopa
AU - Vaidya S
FAU - Cottrell, Jessica A
AU - Cottrell JA
FAU - Graves, Dana
AU - Graves D
FAU - Benevenia, Joseph
AU - Benevenia J
AU - O'Connor JP
AU - Lin SS
LA - eng
DEP - 20121213
TA - J Orthop Res
Research
Society
JID - 8404726
RN - 0 (Hypoglycemic Agents)
RN - 0 (Insulin, Ultralente)
SB - IM
MH - Animals
MH - Biomechanical Phenomena/drug effects/physiology
MH - Blood Vessels/metabolism
MH - Calcification, Physiologic/*drug effects/physiology
MH - Diaphyses/diagnostic imaging/drug effects/physiology
MH - Female
MH - Femoral Fractures/diagnostic imaging/*drug therapy/physiopathology
MH - Femur/diagnostic imaging/drug effects/physiology
MH - Fracture Healing/*drug effects/physiology
MH - Hypoglycemic Agents/pharmacology
MH - Injections, Intralesional
MH - Insulin, Ultralente/*pharmacology
MH - Male
MH - Neovascularization, Physiologic/*drug effects/physiology
MH - Radiography
MH - Rats
MH - Rats, Inbred BB
PMC - PMC6446235
MID - NIHMS1004518
EDAT- 2012/12/15 06:00
MHDA- 2013/05/08 06:00
CRDT- 2012/12/15 06:00
PHST- 2012/08/31 00:00 [received]
PHST- 2012/11/08 00:00 [accepted]
PHST- 2012/12/15 06:00 [entrez]
PHST- 2012/12/15 06:00 [pubmed]
PHST- 2013/05/08 06:00 [medline]
AID - 10.1002/jor.22288 [doi]
PST - ppublish
SO - J Orthop Res. 2013 May;31(5):783-91. doi: 10.1002/jor.22288. Epub 2012 Dec
13.
PMID- 24092898
OWN - NLM
STAT- MEDLINE
DCOM- 20151230
LR - 20150408
IS - 0266-7681 (Linking)
VI - 40
IP - 4
DP - 2015 May
TI - Salvage of a recurrent phalangeal non-union with bone defect using autologous
platelet glue and corticocancellous bone graft.

PG - 422-3
LID - 10.1177/1753193413507521 [doi]
FAU - Lin, C-T
AU - Lin CT
AD - Division of Plastic and Reconstructive Surgery, Department of Surgery, Tri-
Service
General Hospital, National Defense Medical Center, Taipei, Taiwan.
FAU - Chen, S-G
AU - Chen SG
Service
General Hospital, National Defense Medical Center, Taipei, Taiwan.
FAU - Chang, S-C
AU - Chang SC
Service
General Hospital, National Defense Medical Center, Taipei, Taiwan
aarondakimo@yahoo.com.tw.
LA - eng
PT - Case Reports
PT - Letter
DEP - 20131002
PL - England
TA - J Hand Surg Eur Vol
JT - The Journal of hand surgery, European volume
JID - 101315820
SB - IM
MH - Amputation, Traumatic/*surgery/therapy
MH - Blood Platelets
MH - Bone Malalignment/etiology
MH - Bone Plates
MH - Finger Injuries/*surgery/therapy
MH - Finger Phalanges/injuries/*surgery
MH - Fractures, Ununited/etiology/*surgery
MH - Humans
MH - Male
MH - Recurrence
MH - Replantation
MH - Salvage Therapy
MH - Tissue Adhesives
MH - Young Adult
EDAT- 2013/10/05 06:00
MHDA- 2015/12/31 06:00
CRDT- 2013/10/05 06:00
PHST- 2013/10/05 06:00 [entrez]
PHST- 2013/10/05 06:00 [pubmed]
PHST- 2015/12/31 06:00 [medline]
AID - 1753193413507521 [pii]
AID - 10.1177/1753193413507521 [doi]
PST - ppublish
SO - J Hand Surg Eur Vol. 2015 May;40(4):422-3. doi: 10.1177/1753193413507521.
Epub 2013
Oct 2.
PMID- 26728514
OWN - NLM
STAT- MEDLINE
DCOM- 20160808
LR - 20181113
IS - 0009-921X (Print)
VI - 474
IP - 5
DP - 2016 May
TI - Is Arthroscopic Technique Superior to Open Reduction Internal Fixation in the
Treatment of Isolated Displaced Greater Tuberosity Fractures?

PG - 1269-79
LID - 10.1007/s11999-015-4663-5 [doi]
AB - BACKGROUND: Arthroscopic double-row suture-anchor fixation and open reduction
and
internal fixation (ORIF) are used to treat displaced greater tuberosity
fractures,
but there are few data that can help guide the surgeon in choosing between
these
approaches. QUESTIONS/PURPOSES: We therefore asked: (1) Is there a difference
in
surgical time between arthroscopic double-row suture anchor fixation and ORIF
for
isolated displaced greater tuberosity fractures? (2) Are there differences in
the
postoperative ROM and functional scores between arthroscopic double-row
suture
anchor fixation and ORIF for isolated displaced greater tuberosity fractures?
(3)
Are there differences in complications resulting in additional operations
between
the two approaches? METHODS: Between 2006 and 2012, we treated 79 patients
surgically for displaced greater tuberosity fractures. Of those, 32 (41%)
were
considered eligible for our study based on inclusion criteria for isolated
displaced
greater tuberosity fractures with a displacement of at least 5 mm but less
than 2
cm. During that time, we generally treated patients with displaced greater
tuberosity fractures with a displacement greater than 1 cm or with a fragment
size
greater than 3×3 cm with open treatment, and patients with displaced greater
tuberosity fractures with a displacement less than 1 cm or with a fragment
size less
than 3×3 cm with arthroscopic treatment. Fifty-three underwent open treatment
based
on those indications, and 26 underwent arthroscopic treatment, of whom 17
(32%) and
15 (58%) were available for followup at a mean of 34 months (range, 24-28
months).
All patients with such fractures identified from our institutional database
were
treated by these two approaches and no other methods were used. Surgical time
was
defined as the time from initiation of the incision to the time when suture
of the
incision was finished, and was determined by an observer with a stopwatch.
Patients
were followed up in the outpatient department at 6, 12, and 24 weeks, and
every 6
month thereafter. Radiographs showed optimal reduction immediately after
surgery and
at every followup. Radiographs were obtained to assess fracture healing.
Patients
were followed up for a mean of 34 months (range, 24-48 months). At the last
followup, ROM, VAS score, and American Shoulder and Elbow Surgeons (ASES)
score were
used to evaluate clinical outcomes. All these data were retrieved from our
institutional database through chart review. Complications were assessed
through
chart review by one observer other than the operating surgeon. RESULTS:
Patients who
underwent arthroscopic double-row suture anchor fixation had longer surgical
times
than did patients who underwent ORIF (mean, 95.3 minutes, SD, 10.6 minutes vs
mean,
61.5 minutes, SD, 7.2 minutes; mean difference, 33.9 minutes; 95% CI, 27.4-
40.3
minutes; p < 0.001). All patients achieved bone union within 3 months.
Compared with
patients who had ORIF, the patients who had arthroscopic double-row suture
anchor
fixation had greater ranges of forward flexion (mean, 152.7°, SD, 13.3° vs
mean,
137.7°, SD, 19.2°; p = 0.017) and abduction (mean, 146.0°, SD, 16.4° vs mean,
132.4°, SD, 20.5°; p = 0.048), and higher ASES score (mean, 91.8 points, SD,
4.1
points vs mean, 87.4 points, SD, 5.8 points; p = 0.021); however, in general,
these
differences were small and of questionable clinical importance. With the
numbers
available, there were no differences in the proportion of patients
experiencing
complications resulting in reoperation; secondary subacromial impingement
occurred
in two patients in the ORIF group and postoperative stiffness in one from the
ORIF
group. The two patients experiencing secondary subacromial impingement
underwent
reoperation to remove the implant. The patient with postoperative stiffness
underwent adhesion release while receiving anesthesia, to improve the
function of
the shoulder. These three patients had the only reoperations. CONCLUSIONS: We
found
that in the hands of surgeons comfortable with both approaches, there were
few
important differences between arthroscopic double-row suture anchor fixation
and
ORIF for isolated displaced greater tuberosity fractures. Future, larger
studies
with consistent indications should be performed to compare these treatments;
our
data can help inform sample-size calculations for such studies. LEVEL OF
EVIDENCE:
Level III, therapeutic study.
FAU - Liao, Weixiong
AU - Liao W
AD - Department of Orthopedics, General Hospital of PLA, No. 28 Fuxing Road,
Haidian
District, Beijing, China.
FAU - Zhang, Hao
AU - Zhang H
Haidian
FAU - Li, Zhongli
AU - Li Z
Haidian
District, Beijing, China. lizhongli@263.net.
FAU - Li, Ji
AU - Li J
Haidian
LA - eng
DEP - 20160104
TA - Clin Orthop Relat Res
JT - Clinical orthopaedics and related research
JID - 0075674
SB - AIM
SB - IM
CIN - Clin Orthop Relat Res. 2016 May;474(5):1280-2. PMID: 26797910
MH - Adult
MH - Aged
MH - *Arthroscopy/adverse effects
MH - Databases, Factual
MH - Female
MH - *Fracture Fixation, Internal/adverse effects
MH - Humans
MH - Humeral Head/diagnostic imaging/physiopathology/*surgery
MH - Male
MH - Middle Aged
MH - Operative Time
MH - Postoperative Complications/etiology
MH - Radiography
MH - Shoulder Fractures/diagnosis/physiopathology/*surgery
MH - Shoulder Joint/diagnostic imaging/physiopathology/*surgery
MH - Suture Techniques
MH - Time Factors
PMC - PMC4814441
EDAT- 2016/01/06 06:00
MHDA- 2016/08/09 06:00
CRDT- 2016/01/06 06:00
PHST- 2015/08/19 00:00 [received]
PHST- 2015/11/24 00:00 [accepted]
PHST- 2016/01/06 06:00 [entrez]
PHST- 2016/01/06 06:00 [pubmed]
PHST- 2016/08/09 06:00 [medline]
AID - 10.1007/s11999-015-4663-5 [pii]
AID - 4663 [pii]
AID - 10.1007/s11999-015-4663-5 [doi]
PST - ppublish
SO - Clin Orthop Relat Res. 2016 May;474(5):1269-79. doi: 10.1007/s11999-015-4663-
5. Epub
2016 Jan 4.
PMID- 23861888
OWN - NLM
STAT- MEDLINE
DCOM- 20140211
LR - 20181113
IS - 1932-6203 (Linking)
VI - 8
IP - 7
DP - 2013
TI - In vitro and in vivo evaluations of nano-hydroxyapatite/polyamide 66/glass
fibre
(n-HA/PA66/GF) as a novel bioactive bone screw.
PG -e68342
LID -10.1371/journal.pone.0068342 [doi]
LID -e68342
AB -In this study, we prepared nano-hydroxyapatite/polyamide 66/glass fibre
(n-HA/PA66/GF) bioactive bone screws. The microstructure, morphology and
coating of
the screws were characterised, and the adhesion, proliferation and viability
of
MC3T3-E1 cells on n-HA/PA66/GF scaffolds were determined using scanning
electron
microscope, CCK-8 assays and cellular immunofluorescence analysis. The
results
confirmed that n-HA/PA66/GF scaffolds were biocompatible and had no negative
effect
on MC3T3-E1 cells in vitro. To investigate the in vivo biocompatibility,
internal
fixation properties and osteogenesis of the bioactive screws, both n-
HA/PA66/GF
screws and metallic screws were used to repair intercondylar femur fractures
in
dogs. General photography, CT examination, micro-CT examination, histological
staining and biomechanical assays were performed at 4, 8, 12 and 24 weeks

after
operation. The n-HA/PA66/GF screws exhibited good biocompatibility, high
mechanical
strength and extensive osteogenesis in the host bone. Moreover, 24 weeks
after
implantation, the maximum push-out load of the bioactive screws was greater
than
that of the metallic screws. As shown by their good cytocompatibility,
excellent
biomechanical strength and fast formation and ingrowth of new bone, n-
HA/PA66/GF
screws are thus suitable for orthopaedic clinical applications.
FAU - Su, Bao
AU - Su B
AD - Department of Orthopaedics, The First Affiliated Hospital of Chongqing
Medical
University, Chongqing, China.
FAU - Peng, Xiaohua
AU - Peng X
FAU - Jiang, Dianming
AU - Jiang D
FAU - Wu, Jun
AU - Wu J
FAU - Qiao, Bo
AU - Qiao B
FAU - Li, Weichao
AU - Li W
FAU - Qi, Xiaotong
AU - Qi X
LA - eng
DEP - 20130708
TA - PLoS One
JT - PloS one
JID - 101285081
RN - 0 (Ions)
RN - 0 (Nylons)
RN - 0 (fiberglass)
SB - IM
MH - Animals
MH - Biocompatible Materials/*pharmacology
MH - Biomechanical Phenomena/drug effects
MH - *Bone Screws
MH - Cell Shape/drug effects
MH - Dogs
MH - Femur/diagnostic imaging/drug effects/pathology
MH - Humans
MH - Imaging, Three-Dimensional
MH - Ions/blood
MH - Mice
MH - Nanoparticles/ultrastructure
MH - Nylons/*pharmacology
MH - Osteoblasts/cytology/drug effects/ultrastructure
MH - Spectrometry, X-Ray Emission
PMC - PMC3704538
exist.
EDAT- 2013/07/19 06:00
MHDA- 2014/02/12 06:00
CRDT- 2013/07/18 06:00
PHST- 2013/04/09 00:00 [received]
PHST- 2013/05/28 00:00 [accepted]
PHST- 2013/07/18 06:00 [entrez]
PHST- 2013/07/19 06:00 [pubmed]
PHST- 2014/02/12 06:00 [medline]
PST - epublish
SO - PLoS One. 2013 Jul 8;8(7):e68342. doi: 10.1371/journal.pone.0068342. Print
2013.
PMID- 28885383
OWN - NLM
STAT- MEDLINE
DCOM- 20181010
LR - 20181010
IS - 1089-3393 (Linking)
VI - 21
IP - 4
DP - 2017 Dec
TI - Application of a Modified Pedicled Adipofascial Lateral Arm Flap in the
Prevention
and Treatment of Radial Nerve Injuries.
PG - 155-160
LID - 10.1097/BTH.0000000000000171 [doi]
AB - The fasciocutaneous lateral arm flap is a workhorse flap in upper extremity
reconstruction. However, its adipofascial variant is not widely used. The
technique
can be used in various clinical scenarios. The adipofascial flap can be
transposed
to circumferentially wrap the radial nerve with a pliable, vascularized fat
and
fascial envelope, mimicking the natural fatty environment of peripheral
nerves. This
technique has the advantage of providing a scar tissue barrier, a barrier to
hardware irritation and a milieu for vascular regeneration of the nerve.
Suggested
applications include nerve coverage in the setting of posterior humerus
plating to
prevent adhesions; anticipation of bone grafting in the setting of an open
fractures
with bone loss, infection, or with the use of the Masquelet technique; in
revision
total elbow arthroplasty or endoprosthetic humerus replacement; and in the
setting
of neurolysis, repair or nerve grafting. The technique is straightforward and
does
not require microvascular expertise.
FAU - Koehler, Steven M
AU - Koehler SM
AD - Department of Orthopaedic Surgery, Division of Hand and Upper Extremity, Duke
University, Durham, NC.

FAU - Matson, Andrew P
AU - Matson AP
FAU - Mithani, Suhail K
AU - Mithani SK
LA - eng
PL - United States
TA - Tech Hand Up Extrem Surg
JT - Techniques in hand & upper extremity surgery
JID - 9704676
SB - IM
MH - Fasciotomy
MH - Humans
MH - Humeral Fractures/surgery
MH - Radial Nerve/*injuries/*surgery
MH - Surgical Flaps/*blood supply
EDAT- 2017/09/09 06:00
MHDA- 2018/10/12 06:00
CRDT- 2017/09/09 06:00
PHST- 2017/09/09 06:00 [pubmed]
PHST- 2018/10/12 06:00 [medline]
PHST- 2017/09/09 06:00 [entrez]
AID - 10.1097/BTH.0000000000000171 [doi]
PST - ppublish
SO - Tech Hand Up Extrem Surg. 2017 Dec;21(4):155-160. doi:
10.1097/BTH.0000000000000171.
PMID- 27076604
OWN - NLM
STAT- MEDLINE
DCOM- 20160824
LR - 20181202
IS - 0266-7681 (Linking)
VI - 41
IP - 4
DP - 2016 May
TI - Commentary on Implantation of a denatured cellulose adhesion barrier after
plate
osteosynthesis of finger proximal phalangeal fractures: results of a
randomized
controlled trial. E. A. Kappos, P. Esenwein, M. Meoli, R. Meier and J.
Grunert. J
Hand Surg Eur. 2016, 41: 413-20.
PG - 421-2
LID - 10.1177/1753193416635974 [doi]
FAU - Lees, V C
AU - Lees VC
AD - University Hospitals South Manchester, Institute of Inflammation and Repair,
University of Manchester, Manchester, UK vivienlees@live.com.
LA - eng
PT - Comment
PL - England
TA - J Hand Surg Eur Vol
JT - The Journal of hand surgery, European volume
JID - 101315820
RN - 9004-34-6 (Cellulose)
SB - IM
CON - J Hand Surg Eur Vol. 2016 May;41(4):413-20. PMID: 26228699
MH - *Bone Plates
MH - *Cellulose
MH - Finger Phalanges/surgery
MH - Fractures, Bone
MH - Humans
EDAT- 2016/04/15 06:00
MHDA- 2016/08/25 06:00
CRDT- 2016/04/15 06:00
PHST- 2016/04/15 06:00 [entrez]
PHST- 2016/04/15 06:00 [pubmed]
PHST- 2016/08/25 06:00 [medline]
AID - 41/4/421 [pii]
AID - 10.1177/1753193416635974 [doi]
PST - ppublish
SO - J Hand Surg Eur Vol. 2016 May;41(4):421-2. doi: 10.1177/1753193416635974.
PMID- 21902699
OWN - NLM
STAT- MEDLINE
DCOM- 20120306
LR - 20111006
IS - 0143-2885 (Linking)
VI - 44
IP - 11
DP - 2011 Nov
TI - Intentional re-plantation of a vertically fractured tooth repaired with an
adhesive
resin.
PG - 1069-78
LID - 10.1111/j.1365-2591.2011.01922.x [doi]
AB - AIM: To present the successful treatment of a vertically fractured tooth by
intentional re-plantation after root canal treatment and repair with an
adhesive
resin. SUMMARY: Vertical root fracture is a challenging problem in respect of
diagnosis and management options. In this case, a vertically fractured

maxillary
premolar was treated by intentional re-plantation after repairing it with
4-Methacryloxyethyl trimellitate anhyride/methacrylate-tri-n-butyl borane
(4-META/MMA-TBB) resin cement. At the 36-month follow-up, the tooth was
asymptomatic, radiographically sound with reduced deep periodontal pockets
and
vertical bone loss. KEY LEARNING POINTS: • Intentional replantation after
repairing
fractured fragments with an adhesive resin extraorally is a treatment option.
•
Long-term follow-up is necessary to evaluate the outcome of this technique.
CI - © 2011 International Endodontic Journal.
FAU - Unver, S
AU - Unver S
AD - Department of Endodontics, School of Dentistry, Baskent University, Ankara,
Turkey.
dtsaadet@gmail.com
FAU - Onay, E O
AU - Onay EO
FAU - Ungor, M
AU - Ungor M
LA - eng
PT - Case Reports
DEP - 20110908
PL - England
TA - Int Endod J
JT - International endodontic journal
JID - 8004996
RN - 0 (Boron Compounds)
RN - 0 (Methylmethacrylates)
RN - 95508-14-8 (Super-bond)
SB - D
MH - Adult
MH - Bicuspid/injuries
MH - Boron Compounds/therapeutic use
MH - Dental Bonding/methods
MH - Female
MH - Fracture Fixation/methods
MH - Humans
MH - Maxilla
MH - Methacrylates/therapeutic use
MH - Methylmethacrylates/therapeutic use
MH - Resin Cements/*therapeutic use
MH - Root Canal Therapy/methods
MH - *Tooth Extraction
MH - Tooth Replantation/*methods
MH - Tooth Root/*injuries/surgery
EDAT- 2011/09/10 06:00
MHDA- 2012/03/07 06:00
CRDT- 2011/09/10 06:00
PHST- 2011/09/10 06:00 [entrez]
PHST- 2011/09/10 06:00 [pubmed]
PHST- 2012/03/07 06:00 [medline]
AID - 10.1111/j.1365-2591.2011.01922.x [doi]
PST - ppublish
SO - Int Endod J. 2011 Nov;44(11):1069-78. doi: 10.1111/j.1365-2591.2011.01922.x.
Epub
2011 Sep 8.
PMID- 26619948
OWN - NLM
STAT- MEDLINE
DCOM- 20160620
LR - 20160208
IS - 0020-7136 (Linking)
VI - 138
IP - 8
DP - 2016 Apr 15
TI - Novel highly specific anti-periostin antibodies uncover the functional
importance of
the fascilin 1-1 domain and highlight preferential expression of periostin in
aggressive breast cancer.

PG - 1959-70
LID - 10.1002/ijc.29946 [doi]
AB - Periostin (POSTN), a secreted homodimeric protein that binds integrins αvβ3,
αvβ5,
and α6β4, was originally found to be expressed in fetal tissues and in the
adult
upon injury particularly bone fractures due to its role in remodelling and
repair.
Recently it was found to be over-expressed in human breast cancer and a
variety of
other tumour types including head and neck squamous cell carcinoma, where its
overexpression correlates with increased tumour invasion. Progress in

studying its
functional role in tumour pathogenesis has been hampered by the paucity of
antibodies for its specific and sensitive detection. It has proven very
difficult to
obtain monoclonal antibodies (mAbs) against this highly conserved protein but
we
report here that combining infection of mice with lactate dehydrogenase
elevating
virus (LDV), a B cell activating arterivirus, with conjugation of human POSTN
to
ovalbumin as an immunogenic carrier, enabled us to develop six mAbs
recognizing both
human and mouse POSTN and inhibiting its binding to αvβ3 integrin. Two of the
mAbs,
MPB4B1 and MPC5B4, were tested and found to inhibit POSTN-induced migration
of human
endothelial colony forming cells. All six mAbs recognized amino acids 136-51
(APSNEAWDNLDSDIRR) within the POSTN fascilin (FAS) 1-1 domain revealing the
functional importance of this motif; this was further highlighted by the
ability of
aa 136-151 peptide to inhibit integrin-mediated cell migration.
Immunohistochemistry
using MPC5B4, indicated that breast tumour cell POSTN expression was a strong
prognostic indicator, along with tumour size, lymph node, and human epidermal
growth
factor receptor 2 (HER2) status.
CI - © 2015 UICC.
FAU - Field, Sarah
AU - Field S
AD - University of Oxford Branch, Ludwig Cancer Research, Oxford, United Kingdom.
AD - Department of Neurodegenerative Disease, UCL Institute of Neurology, Queen
Square,
London, United Kingdom.
FAU - Uyttenhove, Catherine
AU - Uyttenhove C
AD - Ludwig Cancer Research, Brussels Branch, Brussels, Belgium.
AD - de Duve Institute, Université Catholique De Louvain, Brussels, Belgium.
FAU - Stroobant, Vincent
AU - Stroobant V
FAU - Cheou, Paméla
AU - Cheou P
FAU - Donckers, Dominique
AU - Donckers D
FAU - Coutelier, Jean-Paul
AU - Coutelier JP
FAU - Simpson, Peter T
AU - Simpson PT
AD - The University of Queensland, UQ Centre for Clinical Research, Herston,
Brisbane,
Australia.
AD - Cancer Genetics Laboratory, QIMR Berghofer Medical Research Institute,
Queensland,
Herston, Australia.
AD - The University of Queensland, School of Medicine, Discipline of Molecular &
Cellular
Pathology, Herston, Brisbane, Australia.
FAU - Cummings, Margaret C
AU - Cummings MC
Brisbane,
Australia.
Cellular
AD - Pathology Queensland, The Royal Brisbane & Women's Hospital, Brisbane,
Australia.
FAU - Saunus, Jodi M
AU - Saunus JM
Brisbane,
Australia.
Queensland,
Herston, Australia.
FAU - Reid, Lynne E
AU - Reid LE
Brisbane,
Australia.
Queensland,
Herston, Australia.
FAU - Kutasovic, Jamie R
AU - Kutasovic JR
Brisbane,
Australia.
Queensland,
Herston, Australia.
FAU - McNicol, Anne Marie
AU - McNicol AM
Brisbane,
Australia.
Cellular
FAU - Kim, Ba Reun
AU - Kim BR
AD - Medical Research Centre for Ischemic Tissue Regeneration, School of Medicine,
Pusan
National University, Yangsan, Gyeongsangnam-do, Republic of Korea.
AD - Department of Physiology, School of Medicine, Pusan National University,
Yangsan,
Gyeongsangnam-do, Republic of Korea.
FAU - Kim, Jae Ho
AU - Kim JH
AD - Medical Research Centre for Ischemic Tissue Regeneration, School of Medicine,
Pusan
National University, Yangsan, Gyeongsangnam-do, Republic of Korea.
AD - Department of Physiology, School of Medicine, Pusan National University,
Yangsan,
Gyeongsangnam-do, Republic of Korea.
AD - Research Institute of Convergence Biomedical Science and Technology, Pusan
National
University Yangsan Hospital, Yangsan, Gyeongsangnam-do, Republic of Korea.
FAU - Lakhani, Sunil R
AU - Lakhani SR
Brisbane,
Australia.
Cellular
AD - Pathology Queensland, The Royal Brisbane & Women's Hospital, Brisbane,
Australia.
FAU - Neville, A Munro
AU - Neville AM
AD - Ludwig Cancer Research, New York, NY.
FAU - Van Snick, Jacques
AU - Van Snick J
FAU - Jat, Parmjit S
AU - Jat PS
AUID- ORCID: 0000-0002-3354-0594
AD - Department of Neurodegenerative Disease, UCL Institute of Neurology, Queen
Square,
London, United Kingdom.
AD - MRC Prion Unit, UCL Institute of Neurology, Queen Square, London, United
Kingdom.
LA - eng
DEP - 20151221
PL - United States
TA - Int J Cancer
JT - International journal of cancer
JID - 0042124
RN - 0 (Antibodies, Monoclonal)
RN - 0 (Biomarkers, Tumor)
SB - IM
MH - Adult
MH - Aged
MH - Amino Acid Motifs
MH - Animals
MH - *Antibodies, Monoclonal
MH - Antibody Specificity
MH - Binding Sites, Antibody
MH - Biomarkers, Tumor/*analysis
MH - Breast Neoplasms/metabolism/*pathology
MH - Cell Adhesion Molecules/*metabolism
MH - Cell Movement/physiology
MH - Female
MH - Humans
MH - Mice
MH - Middle Aged
MH - Tissue Array Analysis
OTO - NOTNLM
OT - FAS1-1 domain
OT - breast cancer
OT - diagnostic marker
OT - extracellular matrix protein
OT - monoclonal antibodies
OT - periostin
EDAT- 2015/12/02 06:00
MHDA- 2016/06/21 06:00
CRDT- 2015/12/02 06:00
PHST- 2015/04/22 00:00 [received]
PHST- 2015/11/04 00:00 [accepted]
PHST- 2015/12/02 06:00 [entrez]
PHST- 2015/12/02 06:00 [pubmed]
PHST- 2016/06/21 06:00 [medline]
AID - 10.1002/ijc.29946 [doi]
PST - ppublish
SO - Int J Cancer. 2016 Apr 15;138(8):1959-70. doi: 10.1002/ijc.29946. Epub 2015
Dec 21.
PMID- 24531609
OWN - NLM
STAT- MEDLINE
DCOM- 20160328
LR - 20140217
IS - 0970-4388 (Linking)
VI - 32
IP - 1
DP - 2014 Jan-Mar
TI - Biologic restoration of a traumatized maxillary central incisor in a toddler:
a case
report.
PG - 79-82
LID - 10.4103/0970-4388.127068 [doi]
AB - Trauma to the anterior teeth is relatively common in young children and
teenagers.
Traumatized anterior teeth require quick functional and aesthetic repair, and
poses
a challenge to the dental practitioner owing to the lack of co-operation
ceded and
the longer time invested. Reattachment of tooth fragment should be the first
choice
to restoring teeth when a usable fragment is available, since it gives a
psychological and aesthetic advantage over the conventional technique. With
the vast
improvement in adhesive technology, reattachment is definitely a predictable
treatment option for very young children. This paper describes the treatment
of a 2½
year old female child who sustained crown-root fracture, extending
subgingivally, in
primary upper central incisor.
FAU - John, Sheen Ann
AU - John SA
AD - Department of Pedodontics and Preventive Dentistry, PMS College of Dental
Science
and Research, Thiruvanthapuram, Kerala, India.
FAU - Anandaraj, S
AU - Anandaraj S
FAU - George, Sageena
AU - George S
LA - eng
PT - Case Reports
PL - India
TA - J Indian Soc Pedod Prev Dent
JT - Journal of the Indian Society of Pedodontics and Preventive Dentistry
JID - 8710631
SB - D
MH - Female
MH - Humans
MH - Incisor/*surgery
MH - Infant
MH - Maxilla/*pathology
MH - Tooth Injuries/*surgery
EDAT- 2014/02/18 06:00
MHDA- 2016/03/29 06:00
CRDT- 2014/02/18 06:00
PHST- 2014/02/18 06:00 [entrez]
PHST- 2014/02/18 06:00 [pubmed]
PHST- 2016/03/29 06:00 [medline]
AID - JIndianSocPedodPrevDent_2014_32_1_79_127068 [pii]
AID - 10.4103/0970-4388.127068 [doi]
PST - ppublish
SO - J Indian Soc Pedod Prev Dent. 2014 Jan-Mar;32(1):79-82. doi:
10.4103/0970-4388.127068.
PMID- 22854994
OWN - NLM
STAT- MEDLINE
DCOM- 20121024
LR - 20181113
IS - 0021-9355 (Print)
IS - 0021-9355 (Linking)
VI - 94
IP - 15
DP - 2012 Aug 1
TI - Vancomycin-modified implant surface inhibits biofilm formation and supports
bone-healing in an infected osteotomy model in sheep: a proof-of-concept
study.
PG - 1406-15
LID - 10.2106/JBJS.K.00886 [doi]
AB - BACKGROUND: Implant-associated infections contribute to patient morbidity and
health
care costs. We hypothesized that surface modification of titanium fracture
hardware
with vancomycin would support bone-healing and prevent bacterial colonization
of the
implant in a large-animal model. METHODS: A unilateral transverse mid-
diaphyseal
tibial osteotomy was performed and repaired with a titanium locking
compression
plate in nine sheep. Four control animals were treated with an unmodified
plate and
five experimental animals were treated with a vancomycin-modified plate. The
osteotomy was inoculated with 2.5 × 106 colony-forming units of
Staphylococcus
aureus. The animals were killed at three months postoperatively, and implants
were
retrieved aseptically. Microbiologic and histologic analyses, scanning
electron and
confocal microscopy, and microcomputed tomography were performed. RESULTS:
All
animals completed the study. Compared with the treatment cohort, control
animals
exhibited protracted lameness in the operatively treated leg. Gross findings
during
necropsy were consistent with an infected osteotomy accompanied by a florid
and
lytic callus. Microcomputed tomography and histologic analysis of the tibiae
further
supported the presence of septic osteomyelitis in the control cohort. Thick
biofilms
were also evident, and bacterial cultures were positive for Staphylococcus
aureus in
three of four control animals. In contrast, animals treated with vancomycin-
treated
plates exhibited a healed osteotomy site with homogenous remodeling, there
was no
evidence of biofilm formation on the retrieved plate, and bacterial cultures
from
only one of five animals were positive for Staphylococcus aureus.
CONCLUSIONS:
Vancomycin-derivatized plate surfaces inhibited implant colonization with
Staphylococcus aureus and supported bone-healing in an infected large-animal
model.
FAU - Stewart, Suzanne
AU - Stewart S
AD - Department of Clinical Studies, New Bolton Center, School of Veterinary
Medicine,
University of Pennsylvania, Kennett Square, PA 19348, USA.
FAU - Barr, Stephanie
AU - Barr S
FAU - Engiles, Julie
AU - Engiles J
FAU - Hickok, Noreen J
AU - Hickok NJ
FAU - Shapiro, Irving M
AU - Shapiro IM
FAU - Richardson, Dean W
AU - Richardson DW
FAU - Parvizi, Javad
AU - Parvizi J
FAU - Schaer, Thomas P
AU - Schaer TP
LA - eng
TA - J Bone Joint Surg Am
JT - The Journal of bone and joint surgery. American volume
JID - 0014030
RN - 6Q205EH1VU (Vancomycin)
SB - AIM
SB - IM
MH - Animals
MH - Bacterial Adhesion/drug effects
MH - Biofilms/*drug effects
MH - Bone Plates
MH - Coated Materials, Biocompatible
MH - Osteotomy
MH - Prosthesis-Related Infections/*prevention & control
MH - Sheep, Domestic
MH - Staphylococcal Infections/*prevention & control
MH - Staphylococcus aureus/drug effects
MH - Stem Cells/drug effects
MH - Tibia/*surgery
MH - Titanium
MH - Vancomycin/*pharmacology
PMC - PMC3401139
EDAT- 2012/08/03 06:00
MHDA- 2012/10/25 06:00
CRDT- 2012/08/03 06:00
PHST- 2012/08/03 06:00 [entrez]
PHST- 2012/08/03 06:00 [pubmed]
PHST- 2012/10/25 06:00 [medline]
AID - 1221618 [pii]
AID - K00886 [pii]
AID - 10.2106/JBJS.K.00886 [doi]
PST - ppublish
SO - J Bone Joint Surg Am. 2012 Aug 1;94(15):1406-15. doi: 10.2106/JBJS.K.00886.
PMID- 22138532
OWN - NLM
STAT- MEDLINE
DCOM- 20120720
LR - 20120213
IS - 1067-2516 (Linking)
VI - 51
IP - 2
DP - 2012 Mar-Apr
TI - Arthroscopic De Novo NT(®) juvenile allograft cartilage implantation in the
talus: a
case presentation.
PG - 218-21
LID - 10.1053/j.jfas.2011.10.027 [doi]
AB - Osteochondral defects of the talus are a challenging subject facing foot and
ankle
surgeons. The available treatment options have relatively good subjective
outcomes;
however, they are limited by the ability to reproduce hyaline cartilage, the
need
for multiple surgeries, and high morbidity. We present a new technique using
DeNovo
NT(®) juvenile allograft cartilage implantation introduced into a talar
lesion
arthroscopically in a single procedure to repair a posteriomedial talar
osteochondral defects in a healthy, active 30-year-old female. The patient
tolerated
the procedure well. At the 6-month follow-up visit, the patient had returned
to full
activity, and at 24 months, she remained completely pain free.
CI - Copyright © 2012 American College of Foot and Ankle Surgeons. Published by
Elsevier
Inc. All rights reserved.
FAU - Kruse, Dustin L
AU - Kruse DL
AD - Highlands-Presbyterian/St. Luke's Podiatric Medicine and Surgery Residency
Program,
Denver, CO, USA.
FAU - Ng, Alan
AU - Ng A
FAU - Paden, Matthew
AU - Paden M
FAU - Stone, Paul A
AU - Stone PA
LA - eng
PT - Case Reports
DEP - 20111203
PL - United States
TA - J Foot Ankle Surg
JT - The Journal of foot and ankle surgery : official publication of the American
College
of Foot and Ankle Surgeons
JID - 9308427
SB - IM
MH - Adult
MH - *Arthroscopy
MH - Cartilage/*injuries/*transplantation
MH - Female
MH - Fibrin Tissue Adhesive
MH - Fractures, Bone/diagnosis/surgery
MH - Humans
MH - Joint Instability/etiology/surgery
MH - Joint Loose Bodies/etiology
MH - Ligaments, Articular/injuries/surgery
MH - Magnetic Resonance Imaging
MH - Osteoarthritis/etiology
MH - Talus/*injuries/*surgery
MH - Tissue Adhesives
MH - Transplantation, Homologous
EDAT- 2011/12/06 06:00
MHDA- 2012/07/21 06:00
CRDT- 2011/12/06 06:00
PHST- 2010/09/26 00:00 [received]
PHST- 2011/12/06 06:00 [entrez]
PHST- 2011/12/06 06:00 [pubmed]
PHST- 2012/07/21 06:00 [medline]
AID - S1067-2516(11)00592-8 [pii]
AID - 10.1053/j.jfas.2011.10.027 [doi]
PST - ppublish
SO - J Foot Ankle Surg. 2012 Mar-Apr;51(2):218-21. doi:
10.1053/j.jfas.2011.10.027. Epub
2011 Dec 3.
PMID- 22100295
OWN - NLM
STAT- MEDLINE
DCOM- 20120126
LR - 20181201
IS - 0014-5793 (Linking)
VI - 585
IP - 24
DP - 2011 Dec 15
TI - Changes in cell migration of mesenchymal cells during osteogenic
differentiation.
PG - 4018-24
LID - 10.1016/j.febslet.2011.11.014 [doi]
AB - We showed that the migration, morphology and adhesiveness of undifferentiated
mesenchymal cells dramatically changed during osteogenic differentiation. The
migration of these cells was transiently upregulated early in osteogenic

differentiation. At a later stage, migration was decreased but adhesiveness
was
increased. Furthermore, Cdc42 and Rac1 Rho-family small GTPases were
activated at
early stages of differentiation and the phosphorylation level of FAK
decreased as
differentiation progressed. We also showed cell migration was promoted by
inhibition
of the Rho-ROCK-myosin signaling. Finally, using a mouse model of ectopic
bone
formation, we confirmed that treatment with ROCK inhibitor, Y-27632 increased
cell
movement into bone formation sites, resulting in enhanced osteogenesis. These
results provide a new insight into the link between cell migration and
osteogenic
differentiation.
CI - Copyright © 2011 Federation of European Biochemical Societies. Published by
Elsevier
B.V. All rights reserved.
FAU - Ichida, Masanori
AU - Ichida M
AD - Department of Biology, Osaka Medical Center of Cancer and Cardiovascular
Diseases,
Higashinari-ku, Osaka, Japan.
FAU - Yui, Yoshihiro
AU - Yui Y
FAU - Yoshioka, Kiyoko
AU - Yoshioka K
FAU - Tanaka, Takaaki
AU - Tanaka T
FAU - Wakamatsu, Toru
AU - Wakamatsu T
FAU - Yoshikawa, Hideki
AU - Yoshikawa H
FAU - Itoh, Kazuyuki
AU - Itoh K
LA - eng
DEP - 20111115
PL - England
TA - FEBS Lett
JT - FEBS letters
JID - 0155157
RN - 0 (Protein Kinase Inhibitors)
RN - EC 2.7.11.1 (rho-Associated Kinases)
SB - IM
MH - Animals
MH - *Cell Differentiation/drug effects
MH - Cell Line
MH - *Cell Movement/drug effects
MH - Mesenchymal Stem Cells/*cytology/drug effects/enzymology
MH - Mice
MH - *Osteogenesis/drug effects
MH - Protein Kinase Inhibitors/pharmacology
MH - Signal Transduction/drug effects
MH - rho-Associated Kinases/antagonists & inhibitors/metabolism
EDAT- 2011/11/22 06:00
MHDA- 2012/01/27 06:00
CRDT- 2011/11/22 06:00
PHST- 2011/08/24 00:00 [received]
PHST- 2011/10/20 00:00 [revised]
PHST- 2011/11/09 00:00 [accepted]
PHST- 2011/11/22 06:00 [entrez]
PHST- 2011/11/22 06:00 [pubmed]
PHST- 2012/01/27 06:00 [medline]
AID - S0014-5793(11)00839-8 [pii]
AID - 10.1016/j.febslet.2011.11.014 [doi]
PST - ppublish
SO - FEBS Lett. 2011 Dec 15;585(24):4018-24. doi: 10.1016/j.febslet.2011.11.014.
Epub
2011 Nov 15.
PMID- 27526074
OWN - NLM
DCOM- 20160818
LR - 20181113
IS - 1633-8065 (Print)
IS - 1633-8065 (Linking)
VI - 22
IP - 8
DP - 2012 Dec
TI - Dynamic percutaneous repair of the ruptured tendo Achillis.
PG - 709-12
LID - 10.1007/s00590-011-0897-4 [doi]
AB - We have modified the traditional percutaneous repair of the ruptured tendo
Achillis
so to obtain a lower rate of complications than in open repair, a low rate of
re-rupture and an early mobilization and return to full weight bearing and
sport
activities especially in professional sportsmen. We reviewed 80 patients (52
men and
28 women), 10 of which were professional athletes. We have named this
technique
"dynamic percutaneous suture" (DPS). The repair was carried out using 10
micro-incisions, 5 laterals and 5 medial to the posterior aspects of the
tendon with
absorbable suture. We used one suture through the four proximal incisions in
an
8-shaped and one suture through the four distal as well. The patients were
assessed
according to the criteria established by the clinical AOFAS rating score. No
re-rupture or sural nerve damages were observed. In all the treated patients,
the
results obtained were rated from good to excellent. One patient had mild
disturbances of sensibility over the lateral heels (completely resolved in
2 months), and two patients had scar adhesions. The absorbable suture permits
what
we call a "dynamic" healing of the tendon, through an "elastic" fixation of
the two
stumps, as in the healing of a fractured long bone treated with a dynamic
nail
fixation. We so obtained a short immobilization time and an early full motion
and
weight bearing. Return to sport activities was permitted in 8-12 weeks.
FAU - Gaiani, L
AU - Gaiani L
AD - Department of Orthopaedics Surgery, Ospedale Civile Nuovo Santa Maria della
Scaletta, Via Montericco 4, 40026, Imola (Bo), Italy.
FAU - Bertelli, R
AU - Bertelli R
FAU - Palmonari, M
AU - Palmonari M
massimopalmonari@yahoo.it.
AD - Divisione Ortopedia e Traumatologia, Ospedale Civile Nuovo Santa Maria della
Scaletta, Via Montericco 4, 40026, Imola, (Bo), Italy.
massimopalmonari@yahoo.it.
LA - eng
DEP - 20111022
PL - France
TA - Eur J Orthop Surg Traumatol
JT - European journal of orthopaedic surgery & traumatology : orthopedie
traumatologie
JID - 9518037
OTO - NOTNLM
OT - Dinamic
OT - Percutaneous
OT - Rupture
OT - Tendo Achillis
EDAT- 2012/12/01 00:00
MHDA- 2012/12/01 00:01
CRDT- 2016/08/16 06:00
PHST- 2011/02/11 00:00 [received]
PHST- 2011/10/11 00:00 [accepted]
PHST- 2016/08/16 06:00 [entrez]
PHST- 2012/12/01 00:00 [pubmed]
PHST- 2012/12/01 00:01 [medline]
AID - 10.1007/s00590-011-0897-4 [pii]
AID - 10.1007/s00590-011-0897-4 [doi]
PST - ppublish
SO - Eur J Orthop Surg Traumatol. 2012 Dec;22(8):709-12. doi: 10.1007/s00590-011-
0897-4.
Epub 2011 Oct 22.
PMID- 27068836
OWN - NLM
STAT- MEDLINE
DCOM- 20160801
LR - 20171107
IS - 0140-6736 (Linking)
VI - 388
IP - 10039
DP - 2016 Jul 2
TI - In-vivo oesophageal regeneration in a human being by use of a non-biological
scaffold and extracellular matrix.
PG - 55-61
LID - S0140-6736(15)01036-3 [pii]
LID - 10.1016/S0140-6736(15)01036-3 [doi]
AB - BACKGROUND: Tissue-engineered extracellular matrix populated with autologous
pluripotent cells can result in de-novo organogenesis, but the technique is
complex,
not widely available, and has not yet been used to repair large oesophageal
defects
in human beings. We aimed to use readily available stents and extracellular
matrix
to regenerate the oesophagus in vivo in a human being to re-establish
swallowing
function. METHODS: In a patient aged 24 years, we endoscopically placed a
readily
available, fully covered, self-expanding, metal stent (diameter 18 mm, length
120
mm) to bridge a 5 cm full-thickness oesophageal segment destroyed by a
mediastinal
abscess and leading to direct communication between the hypopharynx and the
mediastinum. A commercially available extracellular matrix was used to cover
the
stent and was sprayed with autologous platelet-rich plasma adhesive gel. The
sternocleidomastoid muscle was placed over the matrix. After 4 weeks, stent
removal
was needed due to stent migration, and was replaced with three stents
telescopically
aligned to improve anchoring. The stents were removed after 3·5 years and the
oesophagus was assessed by endoscopy, biopsy, endoscopic ultrasonography, and
high-resolution impedance manometry. FINDINGS: After stent removal we saw

full-thickness regeneration of the oesophagus with stratified squamous
epithelium, a
normal five-layer wall, and peristaltic motility with bolus transit. 4 years
after
stent removal, the patient was eating a normal diet and maintaining a steady
weight.
INTERPRETATION: Maintenance of the structural morphology of the oesophagus
with
off-the-shelf non-biological scaffold and stimulation of regeneration with
commercially available extracellular matrix led to de-novo structural and
functional
regeneration of the oesophagus. FUNDING: None.
FAU - Dua, Kulwinder S
AU - Dua KS
AD - Division of Gastroenterology and Hepatology, Department of Medicine, Medical
College
of Wisconsin, Milwaukee, WI, USA. Electronic address: kdua@mcw.edu.
FAU - Hogan, Walter J
AU - Hogan WJ
AD - Division of Gastroenterology and Hepatology, Department of Medicine, Medical
College
of Wisconsin, Milwaukee, WI, USA.
FAU - Aadam, Abdul A
AU - Aadam AA
AD - Division of Gastroenterology and Hepatology, Northwestern University,
Evanston, IL,
USA.
FAU - Gasparri, Mario
AU - Gasparri M
AD - Division of Cardiothoracic Surgery, Department of Surgery, Medical College of
Wisconsin, Milwaukee, WI, USA.

LA - eng
PT - Case Reports
PT - Video-Audio Media
DEP - 20160408
PL - England
TA - Lancet
JT - Lancet (London, England)
JID - 2985213R
SB - AIM
SB - IM
CIN - Lancet. 2016 Jul 2;388(10039):6-7. PMID: 27068835
CIN - Nat Rev Gastroenterol Hepatol. 2016 Jun;13(6):314. PMID: 27165514
MH - Abscess/*surgery
MH - *Bone Plates
MH - Cervical Vertebrae/*surgery
MH - Device Removal
MH - Endoscopy
MH - Esophageal Diseases/*surgery
MH - Esophagus
MH - *Extracellular Matrix
MH - Humans
MH - Male
MH - Manometry
MH - Mediastinal Diseases/*surgery
MH - Pharyngeal Diseases/*surgery
MH - Spinal Diseases/surgery
MH - Spinal Fractures/*surgery
MH - Stents
MH - Young Adult
EDAT- 2016/04/14 06:00
MHDA- 2016/08/02 06:00
CRDT- 2016/04/13 06:00
PHST- 2016/04/13 06:00 [entrez]
PHST- 2016/04/14 06:00 [pubmed]
PHST- 2016/08/02 06:00 [medline]
AID - S0140-6736(15)01036-3 [pii]
AID - 10.1016/S0140-6736(15)01036-3 [doi]
PST - ppublish
SO - Lancet. 2016 Jul 2;388(10039):55-61. doi: 10.1016/S0140-6736(15)01036-3. Epub
2016
Apr 8.
PMID- 21815576
OWN - NLM
STAT- MEDLINE
DCOM- 20111207
LR - 20110805
IS - 0147-7447 (Linking)
VI - 34
IP - 8
DP - 2011 Aug 8
TI - Comparison of MRI and arthroscopy in modified MOCART scoring system after
autologous
chondrocyte implantation for osteochondral lesion of the talus.
PG - e356-62
LID - 10.3928/01477447-20110627-10 [doi]
AB - Magnetic resonance imaging (MRI) and arthroscopy have frequently been used to
evaluate articular cartilage. Many studies have compared the accuracy of MRI
to that
of arthroscopy. However, there have been no previous comparison studies
between MRI
and arthroscopy in the evaluation of repaired cartilage after autologous
chondrocyte
implantation using the Magnetic Resonance Observation of Cartilage Repair
Tissue
(MOCART) scoring system. The purpose of this study was to compare the results
between MRI and arthroscopy after autologous chondrocyte implantation of an

osteochondral lesion of the talus using a modified MOCART scoring system. Our
study
investigated 27 consecutive cases in 26 patients who underwent follow-up MRI
and
second-look arthroscopy 1 year following autologous chondrocyte implantation
based
on their osteochondral lesion of the talus diagnosis. According to the
comparison
results of those 5 categories, the agreement between MRI and arthroscopy
evaluation
results was statistically significant with good reliability in the categories
of the
degree of defect repair and defect filling, the quality of repaired tissue
surface,
and synovitis. However, the integration with the border zone and the adhesion
category showed poor to moderate reliability. There has been no well-

established
correlation method between arthroscopy and MRI after autologous chondrocyte
implantation of an osteochondral lesion of the talus.
CI - Copyright 2011, SLACK Incorporated.
FAU - Lee, Kyung Tai
AU - Lee KT
AD - Foot and Ankle Clinic, KT Lee’s Orthopedic Hospital, Seoul, Republic of
Korea.
FAU - Choi, Yun Sun
AU - Choi YS
FAU - Lee, Young Koo
AU - Lee YK
FAU - Cha, Seung Do
AU - Cha SD
FAU - Koo, Hyung Mo
AU - Koo HM
LA - eng
DEP - 20110808
PL - United States
TA - Orthopedics
JT - Orthopedics
JID - 7806107
SB - IM
MH - Adolescent
MH - Adult
MH - Arthroscopy/*methods
MH - Cartilage, Articular/injuries/*surgery
MH - Chondrocytes/*transplantation
MH - Female
MH - Graft Survival
MH - Humans
MH - Magnetic Resonance Imaging/*methods
MH - Male
MH - Middle Aged
MH - Reproducibility of Results
MH - Talus/injuries/*surgery
MH - Young Adult
EDAT- 2011/08/06 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/08/06 06:00
PHST- 2011/08/06 06:00 [entrez]
PHST- 2011/08/06 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.3928/01477447-20110627-10 [doi]
PST - epublish
SO - Orthopedics. 2011 Aug 8;34(8):e356-62. doi: 10.3928/01477447-20110627-10.
PMID- 24021742
OWN - NLM
STAT- MEDLINE
DCOM- 20140717
LR - 20131111
IS - 0363-5023 (Linking)
VI - 38
IP - 11
DP - 2013 Nov
TI - Treatment of split-thickness skin graft-related forearm scar contractures
with a
carbon dioxide laser protocol: 3 case reports.
PG - 2164-8
LID - S0363-5023(13)00840-X [pii]
LID - 10.1016/j.jhsa.2013.06.036 [doi]
AB - Split-thickness skin grafts in the forearm can lead to motion restriction and
disability through the dense scarring of the skin and formation of graft-
tendon
adhesions. Three patients were referred for laser treatment of motion-
limiting
scar-associated split-thickness skin grafts to the forearm. All patients had
reached
a plateau in range of motion despite aggressive hand therapy and underwent
serial
laser scar treatments at 6- to 8-week intervals. Treatments were performed in
a
clinic setting and were initiated 2 to 5 months after reconstructive surgery.
Rapid
subjective functional and objective improvements in range of motion were
noted after
laser therapy. Results were cumulative and durable at final follow-up ranging
from
10 to 15 months after the initial treatment. No complications were noted.
Fractionated carbon dioxide laser therapy is a promising adjunct to hand
therapy
when the main restraint to motion is superficial skin scarring and skin-
tendon
adhesions.
CI - Copyright © 2013. Published by Elsevier Inc.
FAU - Kroonen, Leo
AU - Kroonen L
AD - Departments of Orthopedics and Dermatology, Naval Medical Center San Diego,
San
Diego, California. Electronic address: leo.kroonen@med.navy.mil.
FAU - Shumaker, Peter R
AU - Shumaker PR
FAU - Kwan, Julia M
AU - Kwan JM
FAU - Uebelhoer, Nathan
AU - Uebelhoer N
FAU - Hofmeister, Eric
AU - Hofmeister E
LA - eng
PT - Case Reports
DEP - 20130908
PL - United States
TA - J Hand Surg Am
JT - The Journal of hand surgery
JID - 7609631
SB - IM
MH - Adult
MH - Aged
MH - Blast Injuries/surgery
MH - Cicatrix/complications
MH - Contracture/etiology/*surgery
MH - Forearm Injuries/surgery
MH - Fractures, Bone/surgery
MH - Humans
MH - Laser Therapy/*methods
MH - Lasers, Gas/*therapeutic use
MH - Male
MH - Sarcoma/*surgery
MH - Skin Transplantation/*adverse effects
MH - Wrist Joint/physiopathology/surgery
MH - Young Adult
OTO - NOTNLM
OT - Laser
OT - contracture
OT - scar
OT - skin-graft
EDAT- 2013/09/12 06:00
MHDA- 2014/07/18 06:00
CRDT- 2013/09/12 06:00
PHST- 2012/12/06 00:00 [received]
PHST- 2013/06/19 00:00 [revised]
PHST- 2013/06/21 00:00 [accepted]
PHST- 2013/09/12 06:00 [entrez]
PHST- 2013/09/12 06:00 [pubmed]
PHST- 2014/07/18 06:00 [medline]
AID - S0363-5023(13)00840-X [pii]
AID - 10.1016/j.jhsa.2013.06.036 [doi]
PST - ppublish
SO - J Hand Surg Am. 2013 Nov;38(11):2164-8. doi: 10.1016/j.jhsa.2013.06.036. Epub
2013
Sep 8.
PMID- 22702049
OWN - NLM
STAT- MEDLINE
DCOM- 20130215
LR - 20120618
IS - 1002-1892 (Print)
IS - 1002-1892 (Linking)
VI - 26
IP - 5
DP - 2012 May
TI - [Effectiveness of vacuum sealing drainage combined with anti-taken skin graft
for
primary closing of open amputation wound].
PG - 558-62
AB - OBJECTIVE: To observe the effectiveness of vacuum sealing drainage (VSD)
combined
with anti-taken skin graft on open amputation wound by comparing with direct
anti-taken skin graft. METHODS: Between March 2005 and June 2010, 60 cases of
amputation wounds for limbs open fractures were selected by using the random
single-blind method. The amputation wounds were treated with VSD combined
with
anti-taken skin graft (test group, n = 30) and direct anti-taken skin graft
(control
group, n = 30). No significant difference was found in age, gender, injury
cause,
amputation level, defect size, preoperative albumin index, or injury time
between 2
groups (P > 0.05). In test group, the redundant stump skin was used to
prepare
reattached staggered-meshed middle-thickness skin flap by using a drum
dermatome
dealing after amputation, which was transplanted amputation wounds, and then
the
skin surface was covered with VSD for continuous negative pressure drainage
for 7-10
days. In control group, wounds were covered by anti-taken thickness skin flap
directly after amputation, and conventional dress changing was given.

RESULTS: To
observe the survival condition of the skin graft in test group, the VSD
device was
removed at 8 days after operation. The skin graft survival rate, wound
infection
rate, reamputation rate, times of dressing change, and the hospitalization
days in
test group were significantly better than those in control group [ 90.0% vs.
63.3%,
3.3% vs. 20.0%, 0 vs. 13.3%, (2.0 +/- 0.5) times vs. (8.0 +/- 1.5) times, and
(12.0
+/- 2.6) days vs. (18.0 +/- 3.2) days, respectively] (P < 0.05). The patients
were
followed up 1-3 years with an average of 2 years. At last follow-up, the scar
area
and grading, and two-point discrimination of wound in test group were better
than
those in control group, showing significant differences (P < 0.05). No
obvious
swelling occurred at the residual limbs in 2 groups. The limb pain incidence
and the
residual limb length were better in test group than those in control group (P
<
0.05). Whereas, no significant difference was found in the shape of the
residual
limbs between 2 groups (P > 0.05). In comparison with the contralateral
limbs, the
muscle had disuse atrophy and decreased strength in residual limbs of 2
groups.
There was significant difference in the muscle strength between normal and
affected
limbs (P < 0.05), but no significant difference was found in affected limbs
between
2 groups (P > 0.05). CONCLUSION: Compared with direct anti-taken skin graft
on
amputation wound, the wound could be closed primarily by using the VSD
combined with
anti-taken skin graft. At the same time it could achieve better wound
drainage,
reduce infection rate, promote good adhesion of wound, improve skin survival
rate,
and are beneficial to lower the amputation level, so it is an ideal way to
deal with
amputation wound in the phase I.
FAU - Liao, Qiande
AU - Liao Q
AD - Department of Orthopedics, Xiangya Hospital, Central South University,
Changsha
Hunan 410008, PR China.
FAU - Xu, Jian
AU - Xu J
FAU - Weng, Xiao-Jun
AU - Weng XJ
FAU - Zhong, Da
AU - Zhong D
FAU - Liu, Zhiqin
AU - Liu Z
FAU - Wang, Chenggong
AU - Wang C
LA - chi
PT - Randomized Controlled Trial
PL - China
zazhi =
JID - 9425194
SB - IM
MH - Adolescent
MH - Adult
MH - Aged
MH - *Amputation
MH - Arm Injuries/surgery
MH - Female
MH - Fractures, Bone/surgery
MH - Graft Survival
MH - Humans
MH - Leg Injuries/surgery
MH - Male
MH - Middle Aged
MH - Negative-Pressure Wound Therapy/*methods
MH - Single-Blind Method
MH - Skin/injuries
MH - Skin Transplantation/*methods
MH - Surgical Flaps
MH - Surgical Wound Infection/epidemiology/prevention & control
MH - *Wound Healing
MH - Wounds and Injuries/*surgery
MH - Young Adult
EDAT- 2012/06/19 06:00
MHDA- 2013/02/16 06:00
CRDT- 2012/06/19 06:00
PHST- 2012/06/19 06:00 [entrez]
PHST- 2012/06/19 06:00 [pubmed]
PHST- 2013/02/16 06:00 [medline]
PST - ppublish
SO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2012 May;26(5):558-62.

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PMID- 25861724

tendon, peripheral circulation, umbilical cord blood, skin and dental

emphasis on Charcot neuropathy, fifth metatarsal fractures, arthrodesis, and

Medical School, Worcester, MA, USA.

Medical School, Worcester, MA, USA.

leading to fracture consolidation. Periosteum, the tissue located at the

composition of the periosteum defines its roles in bone growth, in cortical

Periostin is a key extracellular matrix component of the periosteum involved

conventional titanium plate when implanted in rat bone defects. Capable of

Research, Shinshu University, Matsumoto, 390-8621, Japan.

Research, Shinshu University, Matsumoto, 390-8621, Japan.

Research, Shinshu University, Matsumoto, 390-8621, Japan.

Research, Shinshu University, Matsumoto, 390-8621, Japan.

Research, Shinshu University, Matsumoto, 390-8621, Japan.

Periostin-deficient periosteum cannot reconstitute a pool of PCs after injury

demonstrating the presence of SSCs within periosteum and the requirement of

specimens were subjected to thermocycling (5°C to 55°C/5000 cycles) and

fracture pattern using a stereomicroscope, and then representative specimens

fracture resistance of the groups. The results of fracture patterns were

composite, and ceramic inlay showed fracture resistance similar to that of

presented high strength by tensile tests, sensitivity to pH and temperature

effects such as infection, inflammation, and pain. As a new biomaterial with

regeneration and consider possible strategies for its application to improve

antigen-antibody interactions, as in in vitro cell adhesion tests, we

compared to control groups at post-surgery day 3, 7, and 14. Co-staining with

osteoclasts and vascular endothelial cells. The positions of these cellular

foramen. Patients were treated and followed in private practice by the

derived factor-1)/CXCR-4 signalling axis. MSCs would then differentiate

biomechanical performance of calcified tissue adhesives, in vivo. We present,

adhesives intended for use in both osseous and osteochondral tissue

(Tisseel(tm)), and a novel, biocompatible, calcium phosphate-based tissue

disparate, with Tisseel failing gradually, while OsStic failed abruptly, as

evaluation methods (i.e. in vivo imaging, histological analysis, etc.).

indirectly influence other cells in their vicinity. To guide MSC infiltration

formation. The development of periostin null mice has allowed to elucidate

Implications on bone repair.

additionally supported the stability of the scaffold in cell culture, and

substitutes. These polymers provide a suitable environment for the growth of

suitable to stimulate osteogenesis in cranial defects, although this process

PAAm/Dex-U hydrogel (HAp-PADH) has extremely high compressive strength

Differentiation of Equine Induced Pluripotent Stem Cells.

interests or personal relationships.

pathways and physiologic effects of current and potential anabolic drugs.

enhance the management of bone quality-related injuries and diseases in the

obtained by both mechanical and thermally point of views. As a result of this

scaffolds able to function as periosteum substitutes can significantly

chitosan-xanthan-based scaffolds for periosteal tissue engineering. Porous

improved osteoinductivity of these materials, indicating that, when used in

9 weeks, histological measurements were made to determine the length of the

osteopontin-mediated mesenchymal stem cell migration in vitro.

completely abrogated by co-addition of the BMP inhibitor noggin. Consistent

treatment as independent variables. RESULTS: The average AROM of the PIP +

obtained in 74 (63%) of 118 fingers by using the Strickland criteria. Old

differentiation and recruitment of OBs. These changes result in an uncoupling

Department of Orthopaedics, College of Medicine, Kaohsiung Medical

Department of Orthopaedics, College of Medicine, Kaohsiung Medical

Department of Orthopaedics, College of Medicine, Kaohsiung Medical

Department of Physiology, College of Medicine, Kaohsiung Medical University,

histopathology observation, and biomechanical test postoperation regularly.

internal fixation using the modified ilioinguinal approach for acetabular

important for surgeons to consider EIA thrombosis as a potential complication

Okayama City, 700-8558, Okayama, Japan. tnoda@md.okayama-u.ac.jp.

recipient-site/graft interface. Graft incorporation to the recipient site was