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Article History: Received 27th October 2013, Revised 17th December 2013, Accepted 18th December 2013.
Abstract: Flax (Linum usitatissimum) is an annual plant of the linaceae family with several biological properties such as
an indirect effect on hair regrowth through the intermediary of γ-glutamyl transpeptidase. The aim of this study was to
investigate its effect on hair growth process in rabbits. Two trials were carried out using two plant forms and administra-
tion routes: oil topical application and seed ingestion. For each trial animals were assigned into two groups, a control and
a tested one. A 10cm/10cm area on the back of each rabbit was limited and every four weeks, 10 hairs were plucked to
measure their lengths and widths, then the hair of the whole limited area was shaved and weighed. After 4 weeks of use,
linseed oil topical application has a significant positive effect on hair width (39.00 µm against 27.2 µm for the control
group). Like linseed oil application, the linseed supplementation showed a significant (P<0.10) beneficial effect on hair
width, this effect appeared at the 12th week of ingestion, and did not stopped 04 weeks after (P<0.5) supplementation
withdraw. Both seed and oil had no significant positive effect on hair length and weight. These results suggest that flax-
seed oil has some hair growth promoting potential. The mechanism of action, and the plant component(s) responsible of
this activity, should be investigated.
Keywords: Linseed, ingestion; linseed oil; topical application; hair growth; rabbits.
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461
Int. J. Med. Arom. Plants Effects of Linium usitatissium on hair growth in rabbit
During 48 hours following linseed oil appli- Table 3: Mean hair width (µm).
cation, animals were observed to detect any skin 4 weeks 08 weeks 12 weeks 16 weeks
irritation. Linseed oil application did not cause CTLi 49.90 45.32 32.60 27.20
any erythema or edema (as indicated by UNO LSI 50.09 37.06 40.30 38.00
H, 1991); this attests that linseed oil is non irri- P value 0.44 0.93 0.096 0.048
tant to rabbit skin.
After 04 weeks of topical application, lin- Hair weight
seed improved hair width but not hair length and As for hair width, after 12 weeks ingestion,
weight. A decrease in hair length was observed hair weight, showed a non significant beneficial
in LSOA group, while its width has significantly effect of linseed which continued 04 weeks after
increased compared to the CTLo group (α<9%) supplementation withdraw. At that time, hair
(Table 1). weight showed an acute decrease. A very signif-
Table 1: Hair characteristics after 04 weeks top- icant positive effect (α<2%) was recorded at the
ical application. 2nd hair harvest compared to the 1st one. This
effect vanished soon after (Table 4).
Hair Length (cm) Width (µm) Weight (gr)
CTLo 2.72 27.17 0.77 Results of the two administrations routes (ie
LSOA 2.38 39.00 1.17 4 weeks of linseed oil topical application and 12
P value 0.74 0.086 0.39
weeks of linseed ingestion) were compared. Oil
application is significantly (α<4%) more effec-
Effect of linseed ingestion on hair growth tive in promoting hair length than the ingestion
of the seeds (Table 5)
Hair length
Compared to the control group, linseed in-
gestion had no beneficial effect on hair length Table 4: Mean weight (g) of shaved hair.
during the 12 weeks of ingestion and 04 weeks 4 weeks 08 weeks 12 weeks 16 weeks
after supplementation withdraw. A significant CTLi 1.25 2.05 0.47 0.77
transient positive effect was recorded in LSI LSI 0.54* 1.47* 0.68 0.24
group at the 8th week compared the 4th one (Ta- P value 0.95 0.77 0.30 0.75
ble 2). * P=0.01
Table 2: Mean hair length (cm). Table 5: Hair characteristics with tow use ap-
plication.
4 weeks 08 weeks 12 weeks 16 weeks
CTLi 1.73 2.74 2.32 2.72 Length (cm) Width (µm) Weight (g)
LSI 1.66* 2.08* 2.04 2.15 LSOA 2.38 39.00 1.17
P value 0.64 0.99 0.80 0.81 LSI 2.04 40.30 0.68
*P=0.006, α< 1% P value 0.035 0.55 0.40
Discussion
Hair width (diameter)
Several authors reported that fur quality de-
During the first 08 weeks, hair mean width
pends on some factors such as gender, environ-
has linearly decreased in the two groups. The
mental conditions, season, photoperiodism and
slight beneficial effect of supplementation was
sampling method (shaving or depilation)
significant (α<10%) after 12 weeks of intake.
(Charlet-Lery et al., 1985; Rochambeau and
However, this effect didn’t last; since it stopped
Vrillon , 1985). This is why animals used in this
04 weeks after supplementation withdraw (cor-
study were of the same sex and breed, and kept
responding to the 5th hair harvest) (α<5%) (Ta-
at the same environmental conditions and during
ble 3).
the same season.
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462
Int. J. Med. Arom. Plants Effects of Linium usitatissium on hair growth in rabbit
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Acknowledgements: Authors are thankful to Faseehuddin, S.K.A., Basavaraj, M. 2007. Ef-
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