Registration no:

Total Number of Pages: 1 B.Pharm

PH.E.5
8th Semester Regular / Back Examination 2015-16
BIOASSAYS
BRANCH: B.PHARM
Time: 3 Hours
Max Marks: 70
Q.CODE: W353
Answer Question No.1 which is compulsory and any five from the rest.
The figures in the right hand margin indicate marks.
Q1 Answer the following questions: (2 x 10)
a) What is clinical trial?
b) What do you mean by dose response curve?
c) Which drug is assayed by pigeon method?
d) Define fliducial limits.
e) Give examples of Quantal bioassay.
f) What is PA2 value and its importance?
g) Define the difference between potency and efficacy.
h) Name the preparations used for bioassay of thyrotrophin.
i) Write the principles of Bioassay.
j) What is Ringer solution?

Q2 a) Write preparation and any method used in the bioassay of (5)

progesterone.
b) Write any method for bioassay of Adrenaline. (5)

Q3 a) Define the microbiological assay of vitamine B12. (5)

b) Write any method for bioassay of Insulin. (5)

Q4 Describe different methods for bioassay of Histamine. (10)

Q5 a) Give short note on bioassay of agonist and antagonist. (5)

b) Write the bioassay of d-tubocurarine. (5)

Q6 a) Describe preparation and any method used in the bioassay of 5 – (5)

Hydroxy tryptamine.
b) Describe any one method for bioassay of oxytocin. (5)

Q7 a) Write any method for bioassay of Digitalis. (5)

b) Describe any method for bioassay of antibiotics. (5)

Q8 Write short notes on any two: (5 x 2)

a) Biological Standardisation.
b) Biological variation.
c) Design of Bioassays.
d) Advantages and disadvantages of Bioassays.