Can Human Milk Become a

Natural Remedy?
Focus : HMO and HAMLET

Dr. dr. Citrakesumasari, M.Kes, SpGK Department of Nutrition

Faculty of Public Health
Hasanuddin University
Dibawakan dalam webinar internasional seri ke-4 Dep.Gizi FKM UNHAS, Makassar 05 Desember 2020
Outline

MILK Therapy

HAMLET in Human Milk

HMO in Human Milk

Department of Nutrition
Faculty of Public Health
Hasanuddin University
 Human Milk
Components

Department of Nutrition Lyons, KE, et al. 2020.Breast Milk, a Source of Beneficial Microbes and Associated
Faculty of Public Health Benefits for Infant Health. Nutrients. 12:1039
Hasanuddin University
• systematic review
• 15 articles
• published between 1
January 2010- 1 January
2019

Department of Nutrition
Faculty of Public Health
Hasanuddin University
 Findings
Benefits of Human Milk

Skin Problems: Antitumoricidal

Atopic Eczema Nipple Umbilical Cord
and Diaper
Eye Problems Effect of
Problems Care
Dermatitis HAMLET

therapeutic
Reduce nipple efficacy of
alternative to
results were not pain in care leads to shorter HAMLET has been
epithelial healers cord separation time demonstrated
consistent breastfeeding
and artificial tears
women through in vitro and
clinical research

Department of Nutrition
Faculty of Public Health
Hasanuddin University
HAMLET in Human Milk
HAMLET: human alpha-lactalbumin made lethal to tumor cells

• HAMLET was defined as a

complex between partially
unfolded a-lactalbumin and
oleic acid

• Potent tumoricidal activity is

found in both HAMLET and
ELOA (equine lysozyme with
oleic acid , and HAMLET has
also shown strong potential
as an antitumor drug in
different in vivo animal models
Department of Nutrition
Faculty of Public Health
and clinical studies.
Hasanuddin University
HAMLET: the anti-cancer

HAMLET induces apoptosis in tumor cells

Mossberg,AK, et al. 2020. Structure and Function of Human a-lactalbumin lethal to tumor cells (HAMLET).
FEBS Journal, 277 (2010) 4614–4625

Intracellular targets for HAMLET

Department of Nutrition
Faculty of Public Health Svensson, M, et al. 2002. HAMLET - A Complex From Human Milk That Induces Apoptosis
Hasanuddin University in Tumor Cells but Spares Healthy Cells
HAMLET: the biological Hydra

(A) The Lernaean Hydra is proposed as a
metaphor for HAMLET. The mythological animal
was said to use its many heads to attack
enemies. If one head was cut off, new heads
would soon emerge.
Department of Nutrition
Faculty of Public Health
Hasanuddin University
(B) So far, HAMLET has been shown to have
several targets in the tumor cells: (i) the
mitochondria, (ii) the proteasomes, (iii) the
endoplasmatic reticulum and (iv) the histones in
the cell nuclei.
 Evidences on HAMLET as anti-cancer
In Vitro Animals Humans
HAMLET topical HAMLET
HAMLET exerts administration of 0.7 administration of 0.7 mM
cytotoxicity to mM in invasive on papillomas for two
over 50 cancer glioblastoma cells, over months led to a 75%
cell lines, a two-month period, led reduction in the number
including cancer to the reduction of of participants allocated
cells resistant to to an intervention group
tumor size and,
apoptosis consequently, a fall in After two years of the
intracranial pressure, intervention, 83% of the
with no associated patients had total
Teixera, et al. 2019. Whey protein in cancer therapy: A
narrative review Pharmacological Research, 144 (2019) toxicity remission of cancer and
245–256

Department of Nutrition
no adverse effects were
Faculty of Public Health
Hasanuddin University
reported
150- 200 struktur HMO
HMO in Human Milk
The Function of HMO
HMO to Prevent NEC*
*Necrotizing Entrocolitis

• necrotizing enterocoliti (NEC) is

one of most common and
devastating intestinal disorders
that affect 5-10% of all very low
birth weight infants

Bode, Lars. 2018. Human Milk Oligosaccarides in the Prevention of Necrotizing Entrocolitis: A
journey from in vitro and in vivo Models to Mother-Infant Cohort Studies. Frontiers in Pediatric,6:385

Department of Nutrition
Faculty of Public Health
Hasanuddin University
 Immunological Effect of HMO

Bacterial
Intestinal
Adhesion and Intestinal Virus Respiratory Virus Enterocolitis Immune Function
Epithelium
Infection
• prevent • HMOs have • prevent • increased • contribute to the • acidic HMOs
adhesion of effects on viral infectivity of survival rates development may
several potential pathogens as influenza viruses and improved and maturation downregulate
pathogens to rotavirus, • HMO pathology scores of the intestinal Th2 responses
epithelial norovirus, and consumption is immune in infants
surfaces in the HIV inversely linked response.
intestine and to respiratory
other organs by infection
acting as decoy
receptors for
bacterial
pathogens like
Campylobacter
or E. colii
 Conclussion
The composition of breastmilk is
unique and provide short- and
long- term benefit for children.

HMO & HAMLET are potential

component for natural remedies

“The death of 823,000 children and 20,000 mothers each year

could be averted through universal breastfeeding, along with
economic savings US$ 300 billion”
The Lancet - Breastfeeding Series 2016
Department of Nutrition
Faculty of Public Health
Hasanuddin University
• “ and mothers (should) breastfeed
their children for a total of two years.
(This sentences) is for those who
wants to complete breast feeding
duration”

• QS. Al-Baqarah:233
Department of Nutrition
Thank You
Faculty of Public Health
Hasanuddin University