Q7. MS ag) ati) ‘independent variable: temperature ; dependent variable: distance travelled by liquid / volume of oxygen taken up ; 1. (because) these are temperatures at which, enzymes are expected to be functioning / woodlice may survive ; 2 (intervals of) 5°C/3°C/2" ax) inert material/ glass beads, of same volume (as woodiice) ; A dead (sterilised) woodlice ayiv) ‘dea that absorption of carbon dioxide allows measurement of uptake in oxygen / AW ; ayy) ‘any 1 from: 1. movement of woodlice ; 2. size/age/ sex, of woodlice ; 3 AVP: poe allow in context of using the same respirometer for each temperature, or separate respirometers set up in the same way for ‘each temperature any 7 of, {dea of adding a fixed volume of carbon dioxide absorbent solution ; 2. dea of adding fed, number /mass, of woodiice to tube 3. idea of method of adding dye solution to capilary tube 4 idea of attaching tubes and making airtight ; 5. idea of placing respirometer tube into a water-bath ata stated temperature ; 6 7 a 9 {dea of leaving apparatus to equilibrate with tap open ; {dea of closing the tap and, marking recording, position of liquid; {dea of measuring movement of dye on the scale for, 5 minutes / a set tim {dea of re-opening tap / using syringe, to allow liquid to equalise and then closing to take another measurement ; 10 idea of a minimum of three measurements (in sequence) and taking a mean 11 idea of repeating experiment at each temperature, e.g. 5°C, 10°C, 15°C, etc ; 12 low tisk experiment ‘medium risk experiment due to (named) carbon dioxide absorbent is harmful and wear gloves ; 13. AVP; qi {dea of converting the distance in om to mm by multiplying by 10 ; volume calculated = volume of oxygen absorbed by woodlice ; divide each of the volumes by time (oxygen uptake per unit time rate of respiration); by ‘axes correctly orientated with labels ; axes have units ; line shows (exponential) increase as temperature increases ; °° Volume of oxygen absorbed ‘/mim? per unit time. ee Temperature? 1) ‘woodlice are using mostly carbohydrate as their energy source ; Ausing a mixed energy source ‘A.sing, protein lipid, under anaerobic conditions. R using, protein / fat only [MStER Topic: 12 of 19 (Respiration) Page 1 of?Q8. MS )() | (approximately) 1 ; A. value in 0.94 to 1 range to allow for mixed CHO and prote yt) | any 7 from: 1 idea of adding a fixed, number / mass, of peas to respirometer (below the shelf with soda lime) ; 2 idea of a control containing dead (sterilised) peas / glass beads of same mass ; 3 idea of inserting bung with tubing with clip and glass tubing with scale so that the apparatus is airtight /AW ; 4 idea of dipping glass tube into coloured liquid 5 idea of placing respirometer tube into a water-bath at a stated temperature in range 13°C to18°C ; 6 idea of (opening clip and) leaving to equilibrate ; 7. idea of closing the clip and marking the position of dye and idea of starting stop-clock immediately ; 8 idea of measuring movement of dye on the scale, for § minutes / at timed intervals ; 9. idea of opening clip and resetting the coloured liquid ; 10. idea of doing minimum of three measurements in sequence and taking @ mean ; 11. idea of removing carbon dioxide absorbent ; 12. idea of again measuring movement of dye on the scale, for § minutes / at timed intervals ; must bo linked to mp1 13. low risk experiment 14 medium risk experiment and carbon dioxide absorbent is harmful and wear gloves ; 15 AVP; (©) | any 3 rom: 1 find radius of glass tubing and use nr (to find surface area) ; 2 multiply xr2by distance moved by liquid with carbon dioxide absorbent to find volume of oxygen used ; 3 multiply x by distance moved by liquid without carbon dioxide absorbent ; ‘4 add together the volume with carbon dioxide absorbent and volume without carbon dioxide absorbent (to find volume of ‘carbon dioxide released) ; ‘A marks on a correct formula 2.g. oxygen consumed = nr distance moved with carbon dioxide absorbent ‘©M) | 1 idea of replacing the sodium hydroxide by (@ named) oxygen absorbent / replacing air with ritrogen ; 2 idea of setting dye position, close to the tube containing peas /half way along the tube ; 3 100k for liquid moving away from the peas (showing that carbon dioxide is produced in anaerobic conditions) ; Mi) tial 2 and the resut, is anomalous / does not fit the pattern ; 2 of 19 (Respiration) Page 2 of 2