Tabel nilai Absorban Rhodamin B pada beberapa kadar untuk pembuatan kurva baku

Kadar Sampel-Blanko
0.25 0.2764
0.5 0.356 0.8 Kurva Baku
1 0.4394
2 0.5429 0.7
3 0.6241
5 0.7141 0.6 y = 0.0879x + 0.3201
R² = 0.9178
R 0.917826726 0.5
A 0.320086454
Series1
B 0.087862236 0.4
Linear (Series1)
SD 0.165599284
RSD 0.336481326 0.3
x 0.49215
0.2

0.1

0
0 1 2 3 4 5 6

Panjang gelombang (nm) Absorban

C1 (2 μg/mL) C2 (5 μg/mL)
545 546
λmaks = 545+546/2 = 545,5nm
 NAMA: Moraganda Kristianto NIM: 20170311001

Kadar Rhodamin B
Waktu (μg/ml) Intravaskular
(menit) I II III IV
0 115 112 112 109 Area=(C1+C2/2)x Δt
30 95 92 92 93 Area terakhir=Konsentrasi Terakhir/K
60 80 79 81 78
75 63 64 65 58
90 57 54 53 49
120 43 42 39 38
240 20 22 25 21

waktu
(menit) Cp I μg/mL AvCp Waktu ΔT AUC lnCp time ln cp
kurva log kadar Rhodamin B Percobaan I vs t dari rute
0 115 105 30 3150 4.744932 0 4.744932
5
intravaskular
30 95 87.5 30 2625 4.553877 30 4.553877
60 80 71.5 15 1072.5 4.382027 60 4.382027 4 y = -0.0075x + 4.7454
75 63 60 15 900 4.143135 75 4.143135 R² = 0.99
3
90 57 50 30 1500 4.043051 90 4.043051
Series1
120 43 31.5 120 3780 3.7612 120 3.7612 2
240 20 20 -240 2.995732 240 2.995732 Linear (Series1)
1

0
0 50 100 150 200 250 300

AUC0-240 13027.5
AUC240∞ 2666.667 y=-0,0075x+4,7454
AUC total 15694.17 y=4,7454+0,0075
k=0,0075
AUCtakhingga=Cpterakhir/k
2666.667

Waktu t vs log c percobaan ii

sampling 5
y = -0.0058x + 4.4782
(menit) CP II AvCp Waktu ΔT AUC lnCp time lnCp 4 R² = 0.9929
0 112 102 30 3060 4.718499 0 4.718499
30 92 85.5 30 2565 4.521789 30 4.521789 3
Series1
60 79 71.5 15 1072.5 4.369448 60 4.369448 2
75 64 59 15 885 4.158883 75 4.158883
1
90 54 48 30 1440 3.988984 90 3.988984
Linear
120 42 32 120 3840 3.73767 120 3.73767 0
(Series1)
240 22 22 -240 3.091042 240 3.091042 0 100 200 300
 AUC0-240 12862.5
AUC240-∞ 3793.103
AUCTotal 16655.6 K=0,0058/jam

waktu 5
(Menit) CIII Avcp waktu Δt AUC lnCp time lncp
4 y = -0.0065x + 4.6681
0 112 102 30 3060 4.718499 0 4.718499 R² = 0.9427
30 92 86.5 30 2595 4.521789 30 4.521789 3
Series1
60 81 73 15 1095 4.394449 60 4.394449 2
Linear (Series1)
75 65 59 15 885 4.174387 75 4.174387
1
90 53 46 30 1380 3.970292 90 3.970292
120 39 32 120 3840 3.663562 120 3.663562 0
0 100 200 300
240 25 25 -240 3.218876 240 3.218876

AUC0-240 12855
AUC240-∞ 3846.154 K=0,0065/jam
AUCTotal 16701.15

waktu 5
(Menit) C IV AvCp waktu Δt AUC lncp time lncp
4 y = -0.0072x + 4.6607
0 109 101 30 3030 4.691348 0 4.691348
3 R² = 0.9583
30 93 85.5 30 2565 4.532599 30 4.532599
Series1
60 78 68 15 1020 4.356709 60 4.356709 2
75 58 53.5 15 802.5 4.060443 75 4.060443 Linear (Series1)
1
90 49 43.5 30 1305 3.89182 90 3.89182
120 38 29.5 120 3540 3.637586 120 3.637586 0
240 21 21 -240 3.044522 240 3.044522 0 100 200 300

AUC0-240 12262.5 μg/mL

∞
AUC240 2916.667 μg/mL K=0,0072
AUCtotal 15179.17 μg/mL