Ashraf Ali

PhD (Analytical Chemistry/Separation Science)

Advanced Separation Science Laboratory, Department of Chemistry & Chemical Engineering, Inha University
Incheon South Korea.
E-mail: ashrafaliswati@gmail.com & ashraf@inha.edu.kr Ph: +82-10-27961986
Author ID (Scopus): https://www.scopus.com/authid/detail.uri?authorId=55992086700
ORCID: http://orcid.org/0000-0003-2472-0195
Date of birth: 1st March 1986, Nationality: Pakistani
PhD (Analytical Chemistry) (March 2014- Dec 2017)
Dissertation title: Development of columns of the world-best separation performance in LC & CEC
Advisor: Prof. Won Jo Cheong,
Department of Chemistry Inha University, Incheon, South Korea.
MS/M.Phil (March 2010- June 2013)
Thesis title: Removal of Cr(VI), Mn(II) and phenol from aqueous medium using chemically modified
banana peel as adsorbent.
Department of Chemistry, University of Malakand, KPK, Pakistan.
M.Sc (March 2007- April 2009)
Thesis title: Grafting Co-polymerization of Acrylonitrile onto cellulose obtained from banana peels.
Department of Chemistry, University of Malakand, KPK, Pakistan.
Research area
➢ Preparation of silica particles
Preparation of porous silica particles, ground particles of silica monoliths, ranging from (5 µm-2 µm)
with proper pore volume, pore size and surface area.
➢ Synthesis of various stationary phases
➢ Synthesis of various stationary phases for the separation of various analytes such as small organic
molecules (benzene and its derivatives), peptides and proteins.
➢ Preparation of C8, C18, and Polystyrene bound phases for RP-HPLC
➢ Preparation of a novel mixed-mode stationary phase (C18-1-[3-(trimethoxysilyl) propyl] urea) for the
separation of peptides and proteins.
➢ RAFT polymerization
Preparation of polystyrene bound phases: immobilization of polystyrene thin layer over silica particles
to prepare polystyrene bound phases which show properties like core-shell particles and show very high
efficiency for the separation of small organic molecules.

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➢ Packing HPLC columns by slurry packing method
Packing of narrow bore micro-columns by sophisticated slurry packing method (various ID 0.5 mm,
1.0 mm and 2.6 mm ID) using our laboratory made stationary phases. We have designed the best
packing hardware for packing narrow-bore stainless steel columns.
➢ Preparation of open tubular capillary columns (OT-CEC)
Open tubular capillary (OT-CEC) columns for the separation of peptides, proteins and tryptic digest of
cytochrome C. I have successfully prepared long OT-CEC columns (1.2 m) and got very high
efficiency, record high in the world (1.5 million/meter) for synthetic peptides and proteins.
➢ LC-MS analysis of peptides and proteins
➢ Organic synthesis
Publications (PhD)
1) A. Ali, F. Ali. W.J. Cheong, Cheap C18-modified Silica Monolith Particles as HPLC Stationary
Phase of Good Separation Efficiency. Bulletin of the Korean chemical society. 36 (2015) 1733–1736.
2) A. Ali, W.J. Cheong, Open tubular capillary column of 50 μm internal diameter with a very high
separation efficiency for the separation of peptides in CEC and LC. J. Sep. Sci. 2017;40: 2654–2661.
(Front cover)
3) A. Ali, W.J. Cheong, Production of Raw and Ligand-modified Silica Monolith Particles in an
Enhanced Scale and their Application in High Performance Liquid Chromatography, Bull. Korean
Chem. Soc. 2017, Vol. 38, 919–927.
4) A. Ali, F. Ali, W.J. Cheong, Sedimentation assisted preparation of ground particles of silica
monolith and their C18 modification resulting in a chromatographic phase of improved separation
efficiency. J. Chromatgr. A 1525 (2017) 79–86.
5) A. Ali, Yune Sung Kim and Won Jo Cheong, A novel mixed-mode stationary phase based on silica
monolith particles for the separation of peptides and proteins in HPLC. Submitted to Anal. Chem
6) A. Ali, Hong Kyeong Cheong, Sun Gen Lin, Won Jo Cheong, Polystyrene bound silica monolith
particles as HPLC stationary phase of excellent separation efficiency, ready for submission
7) A. Ali, W.J. Cheong, Fast analysis using short C18 column packed with partially sub 2 µm silica
monolith particles. In preparation

Patent (Korean)
Title: “Preparation of mixed ligand bound silica monolith particles for the separation of proteomic
samples” application submitted

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Publications MS (Adsorption, Water treatment)
1) A. Ali & K. Saeed, Decontamination of Cr(VI) and Mn(II) from aqueous media by untreated and
chemically treated banana peel: a comparative study, Desalination and Water Treatment, 53:13,
(2015) 3586-3591.
2) A. Ali & K. Saeed, Phenol removal from aqueous medium using chemically modified banana peels as
low-cost adsorbent, Desalination and Water Treatment, 57:24, (2016) 11242-11254.
3) A. Ali, K. Saeed and F. Mabood, Removal of chromium (VI) from aqueous medium using chemically
modified banana peels as efficient low-cost adsorbent. Alexandria Engineering Journal. 55(3) 2016,
2933-2942.
4) A. Ali, Removal of Mn(II) from water using chemically modified banana peels as efficient adsorbent.
Environmental Nanotechnology, Monitoring & Management. 7 (2017), 57–63.

International Conferences (Oral & poster presentations)

1) Ashraf Ali, Won Jo Cheong, New stationary phase made up of C18 bound sub 2 µm porous silica
monolith particles of enhanced separation performance in HPLC. Asian Conference on
Engineering and Natural Sciences (ACENS-2015), Feb 03-05, 2015, Tokyo Japan.
http://acens-conf.org/index.asp?id=18

2) Ashraf Ali, Won Jo Cheong, C18 bound sub-2um porous silica monolith stationary phase of
enhanced separation performance in liquid chromatography. Global Conference on Engineering
and Applied Science (2015 GCEAS) July 6-8, 2015 Hong Kong.
http://www.gceas-conf.org/index.asp?id=22

3) Ashraf Ali, Won Jo Cheong, Large scale production of C18 bound porous silica monolith
stationary phase of excellent separation performance in HPLC. 43rd International Symposium on
High Performance Liquid Phase Separations and Related Techniques, September 21-25 2015, Beijing.
http://www.hplc2015-beijing.org/

4) Ashraf Ali, Won Jo Cheong. C18 Bound Silica Monolith Particles as HPLC stationary Phase of
Improved Separation Efficiency: Effect of Sedimentation on Separation Efficiency. International
Symposium on Engineering and Applied Science, August 9-11, 2016 Hawaii, USA.
http://iseas.org/site/page.aspx?pid=171&sid=6029&lang=en

5) Ashraf Ali, Won Jo Cheong, Separation of Peptides and Proteins by Open Tubular Capillary
Electro-chromatography. The 9th Singapore International Chemistry Conference 2016, Singapore,
11-14 December 2016. Link: http://www.sicc9.org/
6) Ashraf Ali, Won Jo Cheong, Separation of Synthetic Peptides and Proteins Using Specially
Designed OT-CEC Column. 21st Symposium on the Interface of Regulatory and Analytical Sciences
for Biotechnology Health Products. The Mayflower Hotel, Washington, DC, January 24-26, 2017

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 http://c.ymcdn.com/sites/www.casss.org/resource/resmgr/wcbp/2017_WCBP_Final_Program.pdf12
7) Ashraf Ali, Won Jo Cheong, Sub-2 μm C18 Bound Silica Monolith Particles as HPLC Stationary
Phase of Excellent Separation Efficiency. Analytical Technologies in the Biopharmaceutical Industry
Symposium (AT Europe 2017) at the Radisson Blu Royal Hotel in Brussels, Belgium, March 14-17,
2017. http://c.ymcdn.com/sites/www.casss.org/resource/resmgr/analyt_technol_euro/2017_ate_preliminary_program.pdf
8) Ashraf Ali, Won Jo Cheong, Preparation of C18 bound sub-2 μm silica monolith particles as HPLC
stationary phase of excellent separation efficiency, 41st International Symposium on Capillary
Chromatography and the 14th GCxGC Symposium (ISCC & GCxGC 2017) held in Fort Worth, TX
USA from May 14-19, 2017.
https://static1.squarespace.com/static/573b22ce7c65e4d422bdb245/t/59131673db29d6c7825d5cc2/1494423156111/ISCC+P
oster+Assignments+By+Last+Name.pdf
9) Ashraf Ali, Won Jo Cheong, Separation of Synthetic Peptides by Open Tubular Capillary Electro
chromatography, 41st International Symposium on Capillary Chromatography and the 14th GCxGC
Symposium (ISCC & GCxGC 2017) held in Fort Worth, TX USA from May 14-19, 2017.
https://www.isccgcxgc.com/

10) Ashraf Ali, Genlin Sun, Won Jo Cheong, Polystyrene bound silica monolith particles as HPLC
stationary phase of excellent separation efficiency, 46th International Symposium on High
Performance Liquid Phase Separations and Related Techniques, which will be held on November 5-9,
2017 at the International Convention Center, Jeju, Korea. http://www.hplc2017-jeju.org/
11) Invited talk in (ISCC 2018, Italy) Younger Scientists Session
Very high efficiency Open tubular capillary column for the separation of peptides and tryptic
digest of Cytochrome C. http://iscc42.chromaleont.it/4DAction/web_home_item/17
Local conferences (South Korea)
1) Ashraf Ali, Won Jo Cheong, C18 bound porous silica monolith stationary phase: A step forward
in the development of disposable HPLC micro column. 117 general meeting of Korean Chemical
Society, Oct 14-16, 2014, Daegu, South Korea. http://new.kcsnet.or.kr/eng_news/21161
2) Ashraf Ali, Won Jo Cheong, C18 bound sub 2 µm Porous Silica Monolith Particles as HPLC
Stationary Phase of excellent separation efficiency. Korean Chemical Society conference, Oct 15-
17, 2014, Gwanju, South Korea. http://new.kcsnet.or.kr/eng_news/17950
3) Ashraf Ali, Won Jo Cheong, Preparation of C18 bound silica monolith particles as HPLC
stationary phase of improved separation efficiency. Korean chemical society meeting, October
12~14, 2016, BEXCO, Busan, South Korea. https://new.kcsnet.or.kr/eng_meet.

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Awards and honors
❖ Selected for full scholarship (Jungseok International Scholarship Program) for doctoral study (PhD in
Chemistry) in 2014 from Inha University, Incheon South Korea.
❖ Travel grant award of 1000 USD from California advanced separation science society (CASSS) to
attend 43rd International Symposium on High Performance Liquid Phase Separations and Related
Techniques, in Beijing, September 2015. http://www.casss.org/default.asp/
❖ Travel grant award of 1250 USD from California advanced separation science society (CASSS) to
attend 21st Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology
Health Products (WCBP 2017) which is being held at the Mayflower Hotel in Washington, D.C.
❖ Travel grant award of 1250 USD from California advanced separation science society (CASSS) to
attend Analytical Technologies in the Biopharmaceutical Industry Symposium (AT Europe 2017)
March 2017 at the Radisson Blu Royal Hotel in Brussels, Belgium.
http://c.ymcdn.com/sites/www.casss.org/resource/resmgr/analyt_technol_euro/2017_ate_preliminary
_program.pdf
❖ Travel grant award of 1000 Euros from Chromaleont (Separation Science Foundation Research and
Training Center with University of Messina, Italy) to attend 41st International Symposium on Capillary
Chromatography and the 14th GCxGC Symposium (ISCC & GCxGC 2017) held in Fort Worth, TX
USA from May 14-19, 2017.

Book
Banana peels: A green & efficient adsorbent for heavy metals & phenols, LAP LAMBERT Academic Publishing (2016-
08-02) ISBN-13:978-3-659-90608-4 ISBN-10: 3659906085 EAN:9783659906084 https://www.amazon.com/Banana-
peels-efficient-adsorbent-phenols/dp/3659906085

Reviewer of scientific journals

CLEAN – Soil, Air, Water (WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
Desalination and Water Treatment (Tayler & Francis), Clean Technologies and Environmental Policy, Journal of
Separation Science, Journal of Macromolecular Science, Part A: Pure and Applied Chemistry
Work experience
Teaching assistant for seven semesters during PhD
One year work experience as quality control analyst in Swat pharmaceutical company
References (references will be provided upon request)