Professional Documents
Culture Documents
MARC S. MICOZZI
11
Introduction
The field of taphonomy has been actively considered in the forensic context for only about
one decade. Many taphonomic studies have been carried out and observations made in an
effort to further archaeologic interpretations of past human behavior and to make such
interpretations more scientific. These observations and studies are now helping to make the
estimation of postmortem interval in the forensic context more scientific (Micozzi 1991).
When we first used the term “forensic taphonomy” in a window display at the National
Museum of Health and Medicine, Armed Forces Institute of Pathology, during 1986–1987,
the term was not familiar to many forensic scientists. This volume and others are serving to
make the concepts and terminology of taphonomy more commonplace and useful in the
forensic sciences.
The baseline temperature for most studies of taphonomic processes has been room
temperature or within middle ranges. Changes in temperature tend to alter the rate but not
the fundamental character of taphonomic change (Micozzi 1986, 1991). In frozen environ-
ments, however, the types of changes may be altered substantially, while the rates of change
may slow to virtually zero. Freezing will, of course, preserve soft tissue while in the frozen
state. After thawing, however, the effects of prior freezing may accelerate certain postthaw
decompositional changes, while slowing others.
Johnson (1978) have illustrated zones of continuous and discontinuous permafrost world-
wide, and maximum depths of frost penetration in seasonally frozen ground in the U.S.
(Figures 1 and 2).
desiccation required is minimal. Below 0°C, no desiccation is required (given sustained tem-
peratures) since freezing is the most effective preservative technique. Prior freezing also retards
bacterial growth after thawing at ambient temperature (Micozzi 1986).
It must be considered that the temperature of decomposing soft tissue may differ widely
from the ambient air or soil temperature, due to the creation of an internal microenvironment
by the action of bacteria (Payne 1965; Rodriguez and Bass 1983, 1985). At higher temperatures,
Figure 2 Maximum depths of frost penetration in seasonally frozen ground in the U.S.
desiccation occurs more rapidly but bacterial growth is also greater. Thus the outcome of this
balance at a given temperature depends upon humidity.
Different geographic regions are subject to these processes to varying extents, and a site-
specific index of pedoturbation may be developed with respect to climate, rainfall, seasonality,
latitude, and other factors. The predominance of these processes may be estimated for a given
site at a given time, but the temporal dimension must be added to determine changes in
climate over time. Various combinations of processes may lead to specific feature formation
in various regions. Gelifluction lobes occur at high latitudes and altitudes due to the action
of graviturbation, cryoturbation, and secondary aquaturbation.
In addition to specific cryoturbative processes, whether or not a region is subject to frost
quantitatively alters the character of many other pedoturbative processes. In frost zones,
graviturbation will be manifest as gelifluction (rather than solifluction), as well as soil creep.
Aquaturbation will be differentially manifest as cryostatic rather than artesian processes. The
occurrence of these various specific topographic features at a given site may help identify the
soil pedoturbative processes in operation over time.
There may also be subregional, site-specific variations in pedoturbative processes based
upon topography and drainage. Cryoturbation causes resurfacing, while faunalturbation
causes burial. In well-drained soil, faunal alterations predominate over cryoturbation, while
in poorly drained soils, the opposite will pertain. As a result of all these processes, remains
may alternatively sink into soil, concentrate into layers at various depths, become reoriented,
thrust to the surface, or move horizontally on a plane or downslope, within soil. Processes
occurring in short time-frame windows are superimposed upon the substrate of more gradual
activities, and instantaneous events (seismiturbation or earthquakes) and rapid processes
(graviturbation or landslides) are superimposed upon both.
Baseline
Baseline necropsies at 0 h revealed differences between the “fresh” and “frozen” animals. The
freshly killed animals had normal gross and microscopic examinations, and the internal organs
Day Two
Remaining pairs of animals were observed undisturbed in the field after 2 days (48 h). Insects
(Hymenoptera, Coleoptera) and insect larvae were concentrated around carcasses and the
surrounding areas. All frozen–thawed animals manifested greater external aerobic decompo-
sition (decay) than the fresh-killed controls assessed by greater percent external hair loss,
greater percent skin mummification, and greater percent loss of facial features. For the pair
examined, the upper surface of the fresh-killed animal was intact (left eye present) and fully
articulated. The undersurface (right) showed massive abdominal distention. The upper surface
of the frozen–thawed animal showed focally denuded fur (left eye absent), partially mummi-
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fied skin, and numerous ectoparasites. The undersurface was completely denuded, with skin
focally decayed and flat abdominal wall. Internally, the freshly killed animal showed greater
anaerobic decomposition (putrefaction) of abdominal fat and viscera, massive gastrointestinal
distention, and “moth-eaten” lungs (right more than left). Insect larvae were present only in
the anus and vagina. By contrast, in the frozen–thawed animal internally, the abdominal
organs were preserved, lungs were intact, and gastrointestinal tract was not distended. How-
ever, there were insect larvae in the oral cavity, tracheobronchial tree, and abdominal wall, as
well as anal and vaginal external orifices. Microscopically, the fresh animal showed histologic
preservation of the skin, superficial insect larvae, marked autolysis of liver and other viscera,
and bacterial overgrowth in the gastrointestinal tract.
The frozen animal showed decay of skin muscle, larvae in deep tissues, partial preservation
of viscera, intact pulmonary and biliary trees, and bacterial overgrowth in the upper airway.
Weight change in the fresh animal was 2% loss, and in the frozen 8% loss (including biomass).
Day Four
After 4 days (96 h), both remaining pairs of animals displayed advanced decomposition with
partial mummification and skeletonization, and insect larvae in abdominal cavities. Internally,
abdominal and thoracic viscera were grossly absent in the fresh animal necropsied at this
interval. The skeleton was fully articulated, except for the temporomandibular joint (TMJ).
Insect larvae were present only in the right (ground contact) posterior thoracic region. In the
frozen animal, internal organs were still partially preserved, but insect larvae were present
throughout. The skeleton had undergone partial disarticulation (at TMJ, cervical vertebrae,
symphysis pubis, and right knee), with forelimbs and thoracic cage completely articulated.
Microscopic examination revealed extensive decay, large gram-positive bacterial rods, and
multiple insect larvae. Weight loss was 83% of baseline in each animal at this stage.
Day Six
After 6 days (144 hours), the remaining pair of animals showed no insects or insect larvae on
the carcasses or in the surrounding areas. There was mummification and partial skeletoniza-
tion. Partial disarticulation processes in the fresh animal (TMJ, atlantooccipital, cervical, and
lumbar vertebrae, sternocostal junctions, symphysis pubis) continued to be less advanced
than in the frozen animal (TMJ, cervical and lumbar vertebrae, costovertebral, sternocostal,
lumbosacral, sacroiliac, pelvis-hip). The fresh animal had forelimbs fully articulated, but
remnants of viscera were still present. Weight loss in the fresh animal was 72%, and in the
frozen 87%.
perforation in the head would allow entrance of organisms from the outside. Moreover, if the
body had indeed been frozen, the head may have decomposed more rapidly because of rapid
thawing due to its small size. Tissue sections were evaluated for the presence of ice crystal artifacts
in an attempt to confirm that the body may have been intentionally frozen prior to discarding
it. Careful examination of heart muscle sections revealed features suggestive of ice crystal artifacts.
The body was subsequently identified as that of a man last seen alive about 2¼ years
prior to the supposed time of death. This was fully corroborated almost 3 years later, when
the New Jersey Attorney General’s Office held a suspect named Richard Kuklinski in connec-
tion with a series of homicides, including this case. They indicated that the victim’s body had
been frozen, because one of their informants provided them with a sworn statement that he
had observed a body hanging in a freezer compartment within Kuklinski’s North Bergen, NJ,
warehouse. This case was responsible for Kuklinski being dubbed the “Iceman,” although this
was purportedly the only victim that he froze.
The experimental study of laboratory animals frozen for a period of time after death,
and then thawed, showed decomposition more marked externally than internally (Micozzi
1986). This study supported the preliminary observation and hypothesis proposed by Zugibe
and Costello (1993) at the time of autopsy. The finding of ice crystal artifacts in the tissue
provided them additional evidence that the body appeared to have been frozen prior to
dumping it in Rockland County, NY.
The effects of freezing on tissue have been extensively investigated. Zugibe and Costello
(1993) note the ice crystal artifacts are small disruptions of cellular structures within cells
caused by ice crystal formation during freezing and thawing. During the freezing process,
water is transformed into ice crystals with a rigid crystal lattice within the intracellular,
extracellular, and intranuclear spaces, producing artifactual distortion of the tissue if the
crystals are sufficiently large. The morphological features in the tissues can be recognized as
empty spaces. Careful studies also reveal nuclear distortions due to the pressure exerted by
the ice crystals (vacuolation around the nuclei). Repeated freezing and thawing disrupts cell
organelles, releases enzymes, and produces diffusion of solubilizable constituents (Lillie 1965).
The amount of distortion depends on the rate of freezing, the rate of thawing, the number
of crystallation nuclei, the thermal conductivity properties of the tissue, and the tissue size
and shape (Zugibe and Costello 1993).
The “Iceman” case provides a good example that caution must be exercised in interpreting
the time of death in frozen environments. Although the perpetrator attempted to conceal the
time of death in order to confuse the authorities, he was thwarted by careful observation and
reconstruction of the autopsy findings and other ancillary studies (Zugibe and Costello 1993).
In any case involving a decomposing body that has been dumped during the months of the
year when the climate is cool or warm (not freezing), the medical examiner should consider
References
Artamanov, M.L.
1965 Frozen Tombs of the Scythians. Scientific American 212:101–109.
Aufderheide, A.C.
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