Phenol-Chloroform Extraction

Phenol chloroform extraction involves, firstly, cell lysis and DNA release using
sodium dodecylsulfate (SDS) and proteinase K. Next a phenol/chloroform/isoamyl
alcohol mixture is added to the cell lysate to separate the proteins from the DNA.

From: Trends in Food Science & Technology, 2021

Related terms:

Chromatin, Plasmid, Chloroform, Ethanol Precipitation, Copurification, pH, Super-

natant, Digestion

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