L1354/Appendix C/Frame Page 263 Tuesday, April 18, 2000 1:45 AM

Appendix C
Sampling Containers,
Minimum Sample Size,
Preservation Procedures,
and Storage Times
(Adapted from 40 CFR Parts 100-149, July 1, 1992.)

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 L1354/Appendix C/Frame Page 264 Tuesday, April 18, 2000 1:45 AM

Minimum
Measured Sample Maximum Storage Time
Parameter Container Size (mL) Preservation (Recommended/Regulatory)

Acidity. P, G(B) 100 Cool at 4°C. 14 d.

Alkalinity. P, G 200 Cool at 4°C. 14 d.
BOD. P, G 1000 Cool at 4°C. 48 h.
Boron. P 100 None required. 6 months.
Bromide. P, G 100 None required. 28 d.
Carbon, organic, total. G 100 Analyze immediately, or add 28 d.
H3PO4 or H2SO4 to pH < 2;
cool at 4°C.
COD. P, G 100 Analyze as soon as possible, or 28 d.
add H2SO4 to pH < 2, cool at 4°C.
Chlorine, residual. P, G 500 Analyze immediately. Analyze immediately.
Color. P, G 500 Cool at 4°C. 48 h.
Conductivity. P, G 500 Cool at 4°C. 28 d.
Cyanide: total. P, G 500 Add NaOH to pH >l2, 14 d or 24 h
cool at 4°C in dark. if sulfide present.
Cyanide: P, G 500 Add 100 mg Na2S2O3/L. 14 d or 24 h
amenable to if sulfide present.
chlorination.
Fluoride. P 300 None required. 28 d.
Hardness. P, G 100 Add HNO3 to pH <2. 6 months.
Metals, general. P(A), G(A) 500 For dissolved metals filter 6 months.
immediately, add HNO2 to pH <2.
Chromium VI. P(A), G(A) 300 cool at 4°C. 24 h.
Mercury. P(A), G(A) 500 Add HNO2 to pH <2, 4°C, 28 d.
cool at 4°C.
Nitrogen: ammonia. P, G 500 Analyze as soon as possible or 28 d.
add H2SO4 to pH <2,
cool at 4°C.
Nitrate. P, G 100 Analyze as soon as possible, 48 h (28 d for
or cool at 4°C. chlorinated samples).
Nitrate + nitrite. P, G 200 Add H2SO4 to pH <2, 48 h.
cool at 4°C.
Nitrite. P, G 100 Analyze as soon as possible, or 48 h.
cool at 4°C.
Organic, Kjeldahl. P, G 500 Cool at 4°C, add H2SO4 28 d.
to pH <2.
Oil and grease. G, wide-mouth 1000 Add H2SO4 to pH <2, 28 d/28 d.
calibrated. cool at 4°C.

Organic compounds
MBAS. P, G 250 Cool at 4°C. 48 h.
Pesticides. G(S), TFE-lined Cool at 4°C, 1000 mg ascorbic 7 d/7 d until extraction,
cap. acid/L if residual chlorine 40 d after extraction.
present.
Phenols. P, G 500 Cool at 4°C, add H2SO4 to */28 d.
pH <2.
Purgeables by G, TFE-lined 50 Cool at 4°C, add HCl to pH <2, 7 d/14 d.
purge and trap. cap. 1000 mg ascorbic acid/L if
residual chlorine present.
Oxygen, dissolved: G, BOD 300 Analyze immediately, Analyze immediately,
electrode winkler. bottle titration may be delayed 8 h/8 h.
after acidification.
Ozone. G 1000 Analyze immediately. Analyze immediately.
pH. P, G 50 Analyze immediately. Analyze immediately.

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 L1354/Appendix C/Frame Page 265 Tuesday, April 18, 2000 1:45 AM

Minimum
Measured Sample Maximum Storage Time
Parameter Container Size (mL) Preservation (Recommended/Regulatory)

Organic compounds
Phosphate. G(A) 100 For dissolved phosphate, 48 h.
filter immediately,
cool at 4°C.
Silica. P 200 Cool at 4°C, do not freeze. 28 d.
Solids. P, G 200 Cool at 4°C. 7 d/2-7 d.**
Sulfate. P, G 100 Cool at 4°C. 28 d.
Sulfide. P, G 100 Cool at 4°C, add 4 drops 2N 7 d.
zinc acetate/l00 mL, add NaOH
to pH >9.
Temperature. P, G Analyze immediately. Analyze immediately.
Turbidity. P, G Cool at 4°C. 48 h.

* See text in 40 CFR Parts 100–149, July 1, 1992 for additional details. For determinations not listed, use glass or plastic
containers; preferably refrigerate during storage and analyze as soon as possible. Refrigerate = storage at 4°C, in the dark.
P = plastic (polyethylene or equivalent); G = glass; G(A) or P(A) = rinsed with 1 + 1 HNO3; G(B) = glass, borosilicate;
G(S) = glass, rinsed with organic solvents; N.S. = not stated in cited reference; stat = no storage allowed, analyze immediately.
** Environmental Protection Agency, Rules and Regulations. Federal Register 49; No. 209, October 26, 1984. See this citation
for possible differences regarding container and preservation requirements.

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