Proposal Reference No.

: BIRAC/NAARM0147/BIG-17/20

FACE SHEET

Title Of The Proposal

Cost effective Continuous production of omega 3 rich Chlorella sorkniana by using noval closed race way system
Proposal Duration
18 (months)
Thematic Area
Agriculture and allied areas
Sub Thematic Area
Fisheries
Thematic Options
None of these
Keywords
Chlorella sorkniana, PUFA, DHA, EPA, Nutracueticals
Have you sought mentoring/guidance through an Associate Partner?
No
Application Type
Individual
Incubatee Name
Tritonbiosolutions
Incubated At
WE HUB ALEAP
BIG Partner
A-IDEA NAARM
Project Co-ordinator
tritonbiosolutions
Have you applied through another BIG partner(s) in the current call?
No
Is this the same proposal
No
Have you applied for BIG in earlier rounds?
Yes

Same /
Big Action
Reference No. Title Status Reason Sim /
Partner taken
Diff

1/18
 improvisations

2/18
 Cordycepin has a
niche market.
There are already
a few
manufacturers in
India. Projects
were funded by
DBT and BIRAC in
the past and I do
not know the
status of those
projects. The team
does not have
skills in extraction,
purification,
formulation and
marketing of such
products. A few
Large scale milestones like
production isolation,
of screening, medium
Cordycepin optimization are
from already completed.
IKP
Cordyceps Advantages: 1.
Knowledge
militaris by There is scope for
Park,
BIRAC/NAARM0060/BIG-16/20 solid state generation of new
Hyderabad
fermentation IP with the novel
and strain. 2. Media is
capsulation based on
of the alternatives to
ingredient silkworm pupae,
however it uses
pure glucose.
Alternatives to this
can be looked into.
Not Concerns: 1. A
Recommended comparison of
productivity with
known literature
would have been
helpful. Limited
comparison with
existing literature
and market data,
adding that
information would
have made
assessment clear

3/18
 giving a clear
difference between
methods used and
their advantages.
2. The application
suggests better
efficacy of isolated
cordycepin and
higher purity,
however it is
unclear whether
this data been
obtained during
preliminary
studies? No
information
regarding purity
has been provided
to make an
assessment on
this aspect. The
application
mentions the use
of a novel
extraction process
however details
are not given. A
comparison in this
area would have
helped too. 3.
Details about
novelty of the
formulation are not
provided. Addition
of prebiotics is
mentioned. 4.
Patent information
covering similar
technologies has
not been provided.
5. The novelty of
the proposal is not
clear. The
proposal address
a fundamental
process for
production of
Cordycepin using

4/18
 a fermentation
process. There are
some clear gaps in
the proposal that
need to be
addressed. Would
be good to see
details on 1
Product/Process
Differentiators 2
USP 3 Business
potential 4 Path to
Commercialization.
They may respond
to the issues
raised to convince
the evaluating
team. Some
unanswered we gone

questions are through

there. Why solid suggestions

state fermentation made by

when submerged panel and

also is claimed as we are

feasible. Why tray working on Different

fermenter low it for better

productivity in outcome.

place of forced we already

convection column growing in

contractor high smaller

productivity. Not quantities.

recommended with
given comments.

5/18
Total Funding Requirement
INR 41.44 Lakhs

6/18
 PARTICULARS OF THE APPLICANT

Applicant Name : Tritonbiosolutions

Contact Details
Address1: PLOT NO 24 bhavyas srikrishna avenue Address2: PRAGATHINAGAR
Street/Village: PRAGATHINAGAR City/Town: HYDERABAD
State: TELANGANA Country: India
Pincode/Zip: 500090 Landline: 91-040-48504937
Mobile: 91-7981878649 Website:
Fax: --

Brief Background Of The Incubatee

PAN Card
View file
Passports of PI
View file
Are you a promoter shareholder of a company or one of the partners in a LLP(irrespective of the percentage of shares)?
No
Have you been been associated with any other BIRAC funding scheme?
No

Project Coordinators Details

Title Organization TRITON BIOSOLUTIONS
First Name tritonbiosolutions Last Name
Designation principal investigator Gender Male
Email gbreddy1980@gmail.com Landline 91-040-48504937
Mobile 91-7981534904 Resume View file
Passport Copy View file
Are you working as a Faculty
with a teaching/research No
institution

Team Members
Title Mr Organization TRITON BIOSOLUTIONS
First Name sukumar Last Name k
Designation Co Principal Investigator Gender Male
Email sukku4evr@gmail.com Landline --
Mobile 91-9133286963 Resume View file

Team Members
Title Mr Organization TRITON BIOSOLUTIONS
First Name Muralikrishna Last Name N
Designation Team member Gender Male
Email tritonbiosolutions@gmail.com Landline --
Mobile 91-9000768786 Resume View file

Team Members
Title Dr Organization WE HUB ALEAP
First Name Zahoorullah Last Name S.MD

7/18
Designation Co Principal Investigator Gender Male
Email zahoor.pharoah6@gmail.com Landline --
Mobile 91-7729015999 Resume View file

Team Members
Title Dr Organization SRI TANVI
First Name RANJITH Last Name R
Designation advisor Gender Male
Email ranjithku2@gmail.com Landline --
Mobile 91-9912268982 Resume View file

Scientific Advisors
Title Dr Organization vegesna biosolutions
First Name Renu sarath babu Last Name V
Designation MENTOR Gender Male
Affiliation Area(s) of Expertise enzymes and microbial
vegesna biosolutions
technology,decaffeination
Email renusarath@gmail.com Landline --
Mobile 91-9177893456 Resume View file

8/18
 PROPOSAL SUMMARY

1. Proposal Summary [Provide a brief one paragraph overview of the proposal, i.e. the idea and the problem it may solve and
brief project plan.]
The Omega 3 fatty acids from chlorella are predominantly Ecosapentanoic acids EPA and meet the fish Omega 3 fatty acids.
Chlorella can be cultivated with simpler cultivation methods and can accumulate very biomass as well as lipids with simpler
techniques of induction. Unlike Spirulina and other species of microalgae, the digestibility of Chlorella biomass is very high and
provides a very rich source of crude protein which can be used as feed.
Aquaculture is the fast growing sector which has doubled its production in the past few decades. Aquaculture feed and supplements
are always a challenge in the sector due to their high cost. Microalgae play a major role in the aquaculture as they are a part of the
food chain. Similarly, the derivatives of the microalgae i.e. lipids and pigments can be used as feed supplements in aquaculture.
Along with the aquaculture, these omega 3 fatty acids can be used as feed supplements for poultry and large animals also.
The current project aims in the production of EPA and DHA from microalgae by using nitrogen rich cost effective medium in closed
race way system and its applicability as the aquaculture, poultry and large animals feed supplement.

Please upload a concept note explaining the technology with necessary figures and diagrams :
View file

2. Briefly state the Objectives and Proposed Approach

[Describe how the proposed project addresses the problem. Clarify the current status of the innovation.]
The description should cover the following points:
1). Strategy and/or methodology of work.
2). Scope and boundaries of the work, including any issues that will not be covered.
3). Data analysis (sample size,data collection)
In the current scenario, polyunsaturated fatty acids viz. omega 3 fatty acids are well needed in the human diet. As per the World
Health Organization, a person should consume approx. 200 -500 mg of omega 3 fatty acids. Similarly, omega 3 fatty acids have wide
applicability in the fields of pharmaceuticals, aquaculture and veterinary also. In Aquaculture and Veterinary fields, omega 3 fatty
acids can be used as feed which shows the huge scope of PUFA. Though conventional method of extracting EPA ad DHA from
marine fishes is available, several disadvantages have been noticed. When compared to the fishes, microalgae are easy to grow and
posses good PUFA content. The current project proposal is designed on the following objectives:
* The microalgae is identified by using morphological and molecular sequencing studies.
1. Cost effective medium optimization studies for high yield production of EPA and DHA.
** Production optimization is carried out by taking one factor at a time.
2. Lab trial by using the novel medium composition.
3. Development novel extraction process for achieving high Omega 3 fatty acids yield
4. Qualitative and Quantitative estimation of EPA and DHA content
*** The omega 3 fatty acids obtained needs to be tested qualitatively and quantitatively by using HPLC, GCMS etc.
5. In-vivo growth enhancement study
6. Designing of cost effective closed race way system which can be a substitute to photo bioreactors.
7. Large scale production of microalgae, extraction of EPA and DHA and QC check
8. Formulation of the EPA and DHA
9. Usage of left over microalgal biomass a fodder for large animals.

3. Novelty
[Explain how your idea is innovative and how it is different from the existing products in the markets or current
state-of-the-art. Tabular representation of the difference between your idea and the other products in market or competitive
product which are under development will be appreciated. Concrete market data is encouraged.]

9/18
The project proposal laid down has several novel aspects covering and trying to rectify all the drawbacks in the production of
microalgae. This project targets mainly on the novel production strategies which can reduce the production cost with high yield
enhancement. Following are the novel strategies intended to be implemented in the project:\r\n1. Thermostable potent native isolate
of microalgae with high capacity of EPA and DHA production.\r\n2. Both macro and micronutrients play a major role in the high yield
production of EPA and DHA. The conventional medium BG11 used is very costly and it needs to replace with a cost effective medium.
In the current scenario, the nutrients of the BG11 medium will be replaced with the nitrogen, phosphorous and potash fertilizers which
is cost effective.\r\n3. Development of closed race way system with the polythene sheets which can be a substitute for the high cost
photo bioreactors and conventional raceways.\r\n4. Novel extraction process to achieve high EPA and DHA content.\r\n5. Utilization
of left over algal biomass as fodder for the large animals..View file

4. Opportunity
[What is the potential societal and market impact? Provide details of the problem you propose to solve.]
Market summary:
• As a wholesome food ingredient Chlorella sorokina has good demand in Japan and Europe.
• The market for Omega 3 oils is increasing and lower cost of production provides an opportunity to exploit this technology on
a commercial scale.
• As a source of easily digestible protein, vitamins and antioxidants Chlorella can be a replacement to soy and other algal
products. It can be incorporated to meals for undernourished children, women and old aged people.
• On the basis of ingredient, fishmeal represents the largest segment, followed by fish oil, wheat flour, soybean meal and
others. Chlorella can widely replace these ingredients as a feed stock for animal nutrition.
• Based on additives, the market is segregated as vitamin and protein, fatty acid, antibiotics, antioxidants and feed enzyme.

Major opportunity:

Human Functional Foods market:

Chlorella algae is used as a functional food and food ingredient due to its taste profile. The present market for Chlorella as whole
powder is 20000 Tonnes per year and presently the worldwide production is limited to 2000 Tonnes per year. Omega 3 rich chlorella
is expected to have a market of USD 30 Millions in India, Japan and Europe. The market is growing at an AAGR of 12.7 according to
an US report.

In Aquaculture:

Omega 3 fatty acid has huge market potential in the various fields of pharmaceutical, aquaculture and veterinary. In the Indian
Shrimp feed market is projected to reach 1.8 million tonnes by 2024 registering a CAGR of 10 during 2019-2024. Above data shows
huge potential of omega 3 fatty acids i.e. approximately 1,00,000 tonnes.

In Veterinary:
Taking account of 5 states in our country i.e. Tamilnadu, Andhra Pradesh, Maharashtra, West Bengal, Hariyana and Punjab there is
a huge requirement of poultry feed which is around 12 million tonnes for both boilers and layers. Indian animal feed market is
expected to touch INR 898.5 Billion growing at a CAGR of 14.3 during 2019-2024.In this huge market value, the proposed project has
a good capacity to grab the share.

5. Challenges or risk factors associated with the project

[What are the challenges and risk factors that you envision which may affect this project?]
What are the critical success factors/potential barriers?

10/18
The major challenge faced in the proposed project is to develop best cost effective extraction process to achieve high purity product
which is stable and consistent. The cost of technologies such as supercritical CO2 extraction is prohibitive although it provides a very
good quality product. Solvent extraction is a economical alternative but solvent recovery and removal of trace solvents is a
technological challenge. Stability of the product post purification and improving the thermal stability on oxygen sensitivity of the
product are challenges to be overcome. These challenges will be tackled and solutions will be developed by the end of the project.

6. Has any preliminary work been carried out? Give status of work done
If no, please provide the background details.
Native strains of Chlorella species were isolated from Aquaculture tanks, fresh water tanks and river Godavari in Andhra Pradesh and
Telangana states. The strains were cultivated in shakeflasks and screened for their ability to accumulate lipids. The strains having the
ability of producing high biomass above 25 g/L in shake flasks were selected. The PUFA content and Lipid content of the biomass
were analysed and a thermotolerant strain of green unicellular alga Chlorella sorokiana has been isolated from aquaculture water
samples. The isolate was further grown in an optimized medium containing Urea, Ammonium nitrae and Monnoammonium
Phosphates as Nitrogen sources. The role of metal ions like calcium, magnesium and manganese on the biomass production and
lipid accumulation in the cells was investigated. A medium consisting of Triple nitrogen source viz Urea, Ammonium Nitrate and MAP
at 0.1 w/v of culture broth was found to be optimum for highest biomass accumulation 37G/L with a 52 total lipid content on dry weight
basis. A laboratory scale raceway system enclosed with Polypropylene sheet and PVC lining was developed and the efficiency of the
developed raceway was tested. It was found that the lowcost raceway developed could support the growth of chlorella upto 35G/L
biomass. Further work on the enhancement of biomass production in the developed raceway system is being carried out. The low
cost enclosed raceway however is feasible to grow the algae with high cell density and high lipid content. The proposed project
envisages the improvement of process and engineering parameters for a more economical, high yield production of Omega 3 fatty
acids acids through Chlorella sorokiana.

7. Please provide current and expected Technology Readiness Level (TRL)

Current TRL
TRL - 3
preliminary work like identification of thermo stable organism Chlorella soronkiana and lab scale cultivation of organism and small
scale experiment on race way system was done.
Expected TRL
TRL - 7
we are expecting Lipid content of 35 gm/kg dry weight biomass with high purity of EPA & DHA with good market potential.
8. Proposed end-outcomes (Your BIG Project is expected to result in the following end-outcomes ).
A Product for customers
A Product for customers
An intellectual property right for licensing or sale
9. Future Plan of Commercialization
[What do you envision to be the key next step to making impact with this innovation (e.g., sponsored research support,
licensing, venture financing)? What is the time frame?]
Commercialization plan should indicate :
1). Market entry strategy.
2). Timelines and Milestones.
3). Data analysis (sample size,data collection)
BY Analyzing the potential competitors and possible customers there is huge market requirement for PUFA in market.However we
initially concentrate on aquaculture and animal nutrition. We initially concentrate on feed pellets manufacturing companies who needs
huge quantities of PUFA for manufacturing in their feed formulations.As we already mentioned nearly 1 lakh tons fat is needed in
Indian feed formulations for aquaculture. It is mainly in the form of fish oil. As fish oil has its limiting factors like low content of EPA
and DHA ,odour and limited cultivation space and production cost. Crude fish oil is Rs.120/kg in Indian market. with our technology
we can produce it for Rs.50. So there is huge demand we need to met with.

11/18
10. Intellectual Property
i. Does the applicant or the applicant company own any IP related to this project. If yes, give details.(Please mention Patent
Number, Patent Title and Patent Assignee)
NA

ii. List Of Patents That Appear To Cover Any Part Of The Technology Of Interest Or Similar (And Possibly Overlapping)
Technologies And Thereby Restrict The Freedom-To-Operate In The Envisaged Area.
(Please mention Patent Number, Patent Title and Patent Assignee)
NA

iii. If there are patents that are overlapping and may restrict FTO, does the applicant have the required license/s to practise
these inventions for the purposes of the proposed project? Please provide license agreement details if any or provide
information of the proposed next steps to obtain said license/s.
NA

11. Relevant References.

1.Extraction and purification of high-value metabolites from microalgae: essential lipids, astaxanthin and phycobiliproteins *Sara P.
Cuellar-Bermudez, Iris Aguilar-Hernandez, Diana L. Cardenas-Chavez, Nancy Ornelas-Soto, Miguel A. Romero-Ogawa and Roberto
Parra-Saldivar* Cátedra de Bioprocesos Ambientales, Centro del Agua Para América Latina y el Caribe, Instituto
Tecnológico y de Estudios Superiores de Monterrey, Monterrey, Nuevo Leon 64849, Mexico.

2. Mass cultivation of microalgae Chlorella sorokiniana for biomass and lipid production by manpreet singh and monica sachdeva
taggar.

3. potential production of omega fatty acids from Microalgae

Sayeda, M. Abdo1 , Gamila H. Ali1 , Farouk K. El-Baz2 * 1Water pollution Research Department, National Research Centre, 33 El
Bohouth st. former El Tahrirst. -Dokki, Giza, Egypt. 2 Plant Biochemistry Department, National Research Centre, 33 El Bohouth st.
former El T -Dokki, Giza, Egypt. *Corresponding author’s E-mail: fa_elbaz@hotmail.com

4. Recent Advances in Omega-3: Health Benefits, Sources,Products and Bioavailability

Peter D. Nichols 1,*, Alexandra McManus 2, Kevin Krail 3, Andrew J. Sinclair 4, Matt Miller 5
1 Commonwealth Scientific Industrial Research Organization, Food and Nutrition Flagship, Oceans
and Atmosphere Flagship, GPO Box 1538, Hobart, TAS 7000, Australia
2 Centre of Excellence Science Seafood and Health, Curtin University, 7 Parker Place, Technology
Park, WA 6102, Australia E-Mail: A.McManus@curtin.edu.au
3 The Omega-3 Centre, 32A Owen Street, North Bondi, NSW 2026. Australia
E-Mail: kkrail@omega-3centre.com
4 Deakin University, School of Medicine, Waurn Ponds, VIC, 3217, Australia
E-Mail: andrew.sinclair@deakin.edu.au
5 The New Zealand Institute for Plant & Food Research Limited, P.O. Box 5114, Nelson 7010,
New Zealand E-Mail: matt.miller@plantandfood.co.nz
* Author to whom correspondence should be addressed E-Mail: Peter.Nichols@csiro.au
Tel.: +1-61-3-6232-5222.
Received: 8 April 2014 in revised form: 8 September 2014 / Accepted: 9 September 2014 /
Published: 16 September 2014

6.Omega-3 Fatty Acids EPA and DHA: HealthBenefits Throughout Life

1,2Danielle Swanson,3 Robert Block,4 and Shaker A. Mousa3,5*3The Pharmaceutical Research Institute, Albany College of
Pharmacy and Health Sciences, Rensselaer, NY 4Department of Community and Preventive Medicine, and Division of Cardiology,
Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 5C

12/18
12. Please upload declaration document on ethical/legal/safety/regulatory issues involved, if any .
View file

13. Presentation
View file

Any other information relevant to the project

Please Upload the Relevant Document

View file
No File Uploaded
No File Uploaded

OBJECTIVE AND TIMELINES

PROPOSAL OBJECTIVES & WORK PLAN

Objective1: Novel medium optimization for high yield production of EPA & DHA
Methodology/Experimental Design Detailed Work Plan :
Different variables tested viz. temperature, pH,nutrient ingredients micro and macro ,harvesting time etc.by taking one factor at a time
Alternate Strategies:
NA
Objective2: Design of different stressors for high yield production of EPA & DHA
Methodology/Experimental Design Detailed Work Plan :
Different stressors like
co2,dark,and nutrient stress is tested
Alternate Strategies:
NA
Objective3: Designing of novel cost effective enclosed race way system for mass production of micro algae which is substitute for
photo bioreactor and conventional race ways
Methodology/Experimental Design Detailed Work Plan :
Enclosed race way system designed using polypropylene sheet with light source and cotton plug embedded for proper light source
and continuous feeding of nutrients. LABSCALE
Alternate Strategies:
Alternate materials alike PVC & poly acrylene sheet are tested
Objective4: Micro algal biomass collection
Methodology/Experimental Design Detailed Work Plan :
Biomass will be collected by:
1.continuously flocculation by using biological origin flocculants chitosan and moringa seed sxtract
2.belt press method
Alternate Strategies:
Micron sieve ,centrifugation and flocculation by chemical means.
Objective5: Extraction of omega 3 fatty acids EPA & DHA
Methodology/Experimental Design Detailed Work Plan :
Solvent base method
Alternate Strategies:
Supercritical extraction outsourcing
Objective6: Scale up to 10000 lit capacity raceway system
Methodology/Experimental Design Detailed Work Plan :
Built up of 3 raceways capacity of each 3000 lit
Alternate Strategies:
NA

13/18
Objective7: Biomass utilization
Methodology/Experimental Design Detailed Work Plan :
Protein rich biomass can be utilized as aquaculture feed supplement
Alternate Strategies:
Can be used as poultry and cattle feed supplement.

TIME LINES
Activities Month Of Start Of Activity Month Of End Of Activity Indicators Of Progress
OBJECTIVE :novel medium optimization for high yield production of EPA & DHA
Replacement of macronutrients and
conventional nutrient system to
0 3 High yield of biomass 30g/lit
different NPK ,which involves
urea,diammonium phosphate.
Activities Month Of Start Of Activity Month Of End Of Activity Indicators Of Progress
OBJECTIVE :Design of different stressors for high yield production of EPA & DHA
Using one variable at a time system
following stressors are used: High yield EPA & DHA
1.dark 4 6 accumulation 35g/1kg dry
2.co2 biomass
3.nutrient stress
Activities Month Of Start Of Activity Month Of End Of Activity Indicators Of Progress
OBJECTIVE :designing of novel cost effective enclosed race way system for mass production of micro algae which is
substitute for photo bioreactor and conventional race ways
Cost effective enclosed race way with
Easily movable to one place to
light source and cotton plug provisions
7 8 another and high yield biomass
designed and tested for cultivation of
with less contamination.
micro algae.
Activities Month Of Start Of Activity Month Of End Of Activity Indicators Of Progress
OBJECTIVE :micro algal biomass collection
Flocculation using chitosan and
9 10 Achieve 98 flocculation
moringa seed extract.
Belt press method for collection of
10 11 Achieve 80 in continuous process
algal biomass
Activities Month Of Start Of Activity Month Of End Of Activity Indicators Of Progress
OBJECTIVE :extraction of omega 3 fatty acids EPA & DHA
Extraction using different solvents 12 14 Achieve 30-35g/kg dry biomass
Activities Month Of Start Of Activity Month Of End Of Activity Indicators Of Progress
OBJECTIVE :scale up to 10000 lit capacity raceway system
Scaling up the process to 3 *3000 lit Efficacy of scaleup system is
15 17
capacity closed race way systems tested
Activities Month Of Start Of Activity Month Of End Of Activity Indicators Of Progress
OBJECTIVE :biomass utilization
Assessment of nutrient and growth
promoting parameters of leftover Growth parameters and FCR are
18 18
biomass before extraction and after observed in aquatic animals.
extraction.

QUANTIFIABLE MILESTONES

Sl. No Milestone Name Month Of End Of Activity(In Months)

14/18
1 Signing of Contract --
Replacement of macronutrients and conventional nutrient
2 system to different NPK ,which involves urea,diammonium 3
phosphate.
Cost effective enclosed race way with light source and
3 cotton plug provisions designed and tested for cultivation of 8
micro algae.
4 Extraction using different solvents 14
5 Submission Of Report --

15/18
 PROPOSED EQUIPMENTS & ACCESSORIES DETAILS

Details Of Equipment Proposed To Be Acquired Through BIRAC’s Grant

Specific Requirement Estimated

S.No Infrastructure/Equipment Capacity Quantity
In The Project Value(Rs.In Lakhs)
For fabrication of
closed raceway
systems, for purchase
of oil separation
system, protein and
feed ingredient
1 Equipment 3000 3 11.00
separation, drier for
moisture reduction and
downstream
processing, drum
filtration equipment for
biomass separation.
For chemicals, Media
components, Lab ware,
2 consumables 20 20 glassware, reagents for 4.00
analysis, storage of
biomass and oil.
Total :15.00

HUMAN RESOURCES TO BE INVOLVED

Human Resource to be involved with the project

Hired
Age(In
S.No Position Duration Role In Project
Years)
(in
research months)
1 1 M.Sc 2.00 35 18.0 research 0.16 2.88
scholor
EPA & DHA
2 Chemist 1 M.Sc 2.00 35 18.0 0.16 2.88
QUANTIFICATIONSTUDIES
Total
3 helper 1 inter 1.00 25 18.0 Help for research scholors 0.08 1.44
No Of for cleaning and raceway Monthly
4 helper 1 inter 1.00 23 18.0 0.08 1.44
Cost(In
maintance
Positions for advising in extraction Salary(In Lakhs)
5 ADVISOR 1 PhD 5.00 40 12.0 0.25 3.00
Exp.(Years) process Lakhs)
Qualification
6 mentor 1 PhD 8.00 50 18.0 for mentoring 0.10 1.80
Total : 13.44

CONSUMABLES DETAILS

Through BIRAC’s Contribution

Approximate Cost Justification For The
S.No Items Quantity Units(e.g:- g/ml etc.)
(Rs.in lakhs) Requirement
for usage as nutrient
1 chemicals 40 kg 1.50 and for media
preparation

16/18
 for cultivationof
2 nutrient media 10 kg 1.00
microalgae
3 reagents for analysis 10 kg 0.50 for analysis
storage for fats and
4 10 kg 1.00 for storage
oils
Total Amount Required For Consumables: 4.00

JUSTIFICATION FOR OTHER RECURRING HEADS

Through BIRACs Contribution

Other Cost
S.No Justification
(Rs.in lakhs)
for incubation services,For travel to discusions with mentors,
technical discussions with experts in field, submission of
progress reports, symposia and seminars.
For analysis of Fatty acids, characterization of improved strains
1 9.00
and other analytical services, expert charges, fabrication
charges.
To meet unforeseen expenditure, utility charges for equipment
in incubator etc.

17/18
 BUDGET DETAILS

Non Recurring Cost(Rs. In Lakhs)

S.No Equipments/Accessories Total
1 15.00 15.00

Recurring Cost(Rs. In Lakhs)

Human Resources(A) Consumables(B) Other Heads(C) Total(A+B+C)
13.44 4.00 9.00 26.44

OTHER FINANCIAL DETAILS

Have you approached any other organisation/agency for financial support for the present activity? Please give details etc.
no

18/18