G1361A Preparative Pump Reference Manual

Agilent 1100 Series
Preparative Pump
Reference Manual
s1
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Technologies 2000
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Part No. G1361-90000 including, but not
limited to, the implied
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Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Germany
Agilent 1100 Series Preparative Pump
Reference Manual
In This Book
This manual contains technical reference information about the Agilent 1100 Series
preparative pump. The manual describes the following:
• Installation,
• Optimizing performance,
• Troubleshooting,
• Repairing,
• Parts and materials,
• Introduction and theory of operation,
• Screens of local control,
• Specifications and
• Legal, safety and warranty information.
4
Contents
1 Installing the Pump

How to install the preparative pump. 13
Installing the Pump 14

Delivery Checklist 17
Optimizing the Stack Configuration 21
Installing the preparative pump 28
Flow Connections for a single (isocratic) Preparative Pump 31
Flow Connections for a dual (binary gradient) Preparative
Pump 34
Get the System Ready for the First Analysis 38
2 Optimizing Performance
How to optimize the preparative pump to achieve best
performance 41
Hints for Successful Use of the Preparative Pump 42

Solvent Information 43
Prevent Blocking of Solvent Inlet Filters 44
Checking the solvent inlet filters 44
Cleaning the Solvent Filters 45
How to Configure the Seal Wash Function 46
Water or Aqueous Buffer Solutions 46
Organic Solvents 46
How to Optimize the Compressibility Compensation Setting 47
3 Troubleshooting and Test Functions

The preparative pump’s built-in troubleshooting and test
5
Contents
functions 49
Status Indicators and Operation 50

Error Messages 50
Pressure Test 50
Leak Test 50
EMPV Cleaning 51
Status Indicators 52
Power Supply Indicator 53
Instrument Status Indicator 53
Error Messages 55
Time-out 56
Shutdown 57
Remote Time-out 58
Synchronization Lost 59
Leak 60
Leak Sensor Open 61
Leak Sensor Short 62
Compensation Sensor Open 63
Compensation Sensor Short 64
Fan Failed 65
Open Cover 66
Pressure Above Upper Limit 67
Pressure Below Lower Limit 68
Pressure Sensor not Connected 69
Wrong Data from Pressure Sensor 70
Motor Drive Temperature too High 71
Motor Drive Fault 72
Motor Maximum Power Fault 73
6
Contents
Motor Direction 74
Pump Off While Analysis 75
Index Missing 76
Position Error 77
Spurious Index 78
Encoder not Connected 79
Purge Valve (EMPV) Fuse Blown 80
Electro-Magnetic-Proportional-Valve (EMPV) Missing 81
No Pump Head 82
Wait for Volume Timed Out 83
Wait for Pressure Timed Out 84
Composition Cluster Partner Lost 85
Solvent Counter Zero 86
No Motor Motion 87
Seal Wash Current Low 88
Seal Wash 89
Wait for Encoder Position Timed Out 90
24V Supply for External Devices Low 91
Pressure Test 92
Description 92
Positioning the Blank Nut 94
Running the Pressure Test 95
Running the Test from the ChemStation 95
Running the Test from the Control Module 95
Evaluating the Results 97
Potential Causes of Pressure Test Failure 98
Leak Test 99
Description 99
Running the Leak Test 102
Running the Test from the ChemStation 102
7
Contents
Running the Test from the Control Module 102

Evaluating the Results 103
EMPV Cleaning 106
4 Repairing the Pump

Instructions on simple, routine repair procedures as well as
more extensive repairs requiring exchange of internal
parts 109
Simple Repairs 110

Exchanging Internal Parts 110
Using the ESD Strap 112
Using the ESD Strap 112
Overview 113
Simple Repair Procedures 114

Exchanging the Inlet/Outlet Ball Valves 115
Removing an Inlet Ball Valve (Bottom of the Pump Head) 115
Removing an Outlet Ball Valve (Top of the Pump Head) 116
Replacing an Inlet/Outlet Valve 116
Exchanging the Filter Cup 117
Exchanging the Filter Frit 119
Changing the Filter Frit 119
Exchanging the Complete EMPV or just the Valve 121
Changing the valve 121
Exchanging the Complete EMPV 123
Exchanging the Seal Wash Pump Cassette 124
Removing and Disassembling the Pump Head Assembly 125
Reassembling the Pump Head Assembly 128
Exchanging the Optional Interface Board 131
8
Contents
Exchanging Internal Parts 132

Removing the Top Cover and Foam 134
Exchanging the Multi Assembly 136
Exchanging the Preparative Pump Main Board (PPM
Board) 138
Entering the Serial Number 140
Replacing the Preparative Pump’s Firmware 142
Exchanging the Fan 143
Exchanging or Maintaining the Pump Drive 144
Exchanging Power Supply 145
Exchanging the Leak Sensor 146
Exchanging the Seal Wash Pump Drive 147
Exchanging Power Switch or Light Pipe 148
Exchanging Status Light Pipe 149
Assembling the Main Cover 150
Replacing the Top Cover and Foam 151
5 Parts and Materials

Detailed illustrations and lists for identification of parts and
materials 155
Pump Housing and Main Assemblies 156

Solvent Cabinet and Bottle-Head Assembly 159
Hydraulic Path 160
Pump-Head Assembly 162
Electro Mechanical Proportional Valve (EMPV) 164
Multi Assembly Parts 165
Cover Parts 166
Sheet Metal Kit 167
9
Contents
Foam Parts 168

Preparative Pump Basis Kit 169
Preparative Pump Gradient Kit 170
Control Module (G1323B) 171
Power and Status Light Pipes 172
Leak Parts 173
Cable Overview 174

Analog Cables 176
Remote Cables 178
BCD Cables 183
Auxiliary Cable 185
CAN Cable 185
External Contact Cable 186
RS-232 Cable Kit 187
LAN Cables 188
6 Introduction to the Preparative Pump

An introduction to the pump, instrument overview, theory of
operation, external communication and internal
connectors 189
Introduction to the Preparative Pump 190

Hydraulic Path Overview 192
Electrical Connections 195
Instrument Layout 197
Early Maintenance Feedback (EMF) 198
EMF Counters 198
Using the EMF Counters 199
The Electronics 200
10
Contents
Preparative Pump Main Board (PPM) 201

Firmware Description 206
Resident System 206
Main System 206
Firmware Updates 207
Optional Interface Boards 208
BCD Board 208
LAN Board 209
Agilent 1100 Series Interfaces 211
CAN Interface 212
APG Remote Interface 212
RS-232C 214
Setting the 8-bit Configuration Switch 215
Communication Settings for RS-232C Communication 216
Forced Cold-Start Settings 217
Stay Resident Settings 218
The Main Power Supply Assembly 219
7 Control Module Screens for the Preparative Pump

Introduction to the screens available for operation of the Agi-
lent 1100 Series preparative pump with the control
module 221
Major keys on the Agilent 1100 Control Module 222

Screens available from the Analysis view 223
Screens available from the System view 234
Screens available from the Records screen 238
Diagnostics and Tests 244
11
Contents
8 Specifications
Performance specifications of the preparative pump 245
Performance Specifications 246
Legal, Safety and Warranty Information

All Chemical Analysis Products 248
Limitation of Warranty 248
Limitation of Remedies and Liability 249
Responsibilities of the Customer 249
Responsibilities of Agilent Technologies 250
Safety Information 251
General 251
Operation 251
Safety Symbols 252
Lithium Batteries Information 254
Radio Interference 255
Test and Measurement 255
Sound Emission 255
Manufacturer’s Declaration 255
Solvent Information 256
Solvents 256
Agilent Technologies on Internet 257
12
1
1 Installing the Pump
How to install the preparative pump.

Installing the Pump
Installing the Pump
A suitable site environment is important to ensure optimal performance of

the preparative pump.
Power Consideration
The preparative pump power supply has wide-ranging capability (see Table
1). Consequently there is no voltage selector in the rear of the preparative
pump. There are also no externally accessible fuses.
WAR NI N G To disconnect the preparative pump from line power, unplug the power
cord. The power supply still uses some power, even if the power switch
on the front panel is turned off.
WAR NI N G Shock hazard or damage of your instrumentation can result if the

devices are connected to a line voltage higher than specified.
Power Cords
Different power cords are offered as options with the preparative pump. The
female end of each power cord is identical. It is plugged into the power input
socket at the rear of the preparative pump. The male ends of the power cords
are available with different designs. The power cord, which is delivered with
your preparative pump is designed to match the wall socket of your
particular country or region.
WAR NI N G Never operate your instrumentation from a power outlet that has no
ground connection. Never use a power cord other than the power cord
designed for your region.
14
Installing the Pump
Installing the Pump
WAR NI N G Never use cables other than the ones supplied by Agilent Technologies
to ensure proper functionality and compliance with safety or EMC
regulations.
Bench Space
The preparative pump dimensions and weight (see Table 1) allow the
instrument to be placed on almost any laboratory bench. The instrument
requires an additional 2.5 cm (1.0 inch) of space on either side, and
approximately 8 cm (3.1 inches) at the rear for the circulation of air, and
room for electrical connections. Ensure the preparative pump is installed in a
horizontal position.
If a complete Agilent 1100 Series system is to be installed on the bench, make
sure that the bench is designed to carry the weight of all the modules. For a
complete system including multiple pumps, Agilent 220 micro plate sampler
or an LC-MSD it is recommended to position the modules in two or more
stacks.
Environment
Your preparative pump will work within specifications at ambient
temperatures and relative humidity as described in Table 1.
CA UT IO N Do not store, ship or use your preparative pump under conditions where
temperature fluctuations may cause condensation within the preparative
pump. Condensation will damage the system electronics. If your preparative
pump was shipped in cold weather, leave it in its box, and allow it to warm up
slowly to room temperature to avoid condensation
15
Installing the Pump
Installing the Pump
Table 1 Physical Specifications - Preparative Pump
Type Specification Comments
Weight 15.0 kg
Dimensions 200 × 345 × 440 mm
(height × width × depth) (8 × 13.5 × 18 inches)
Line voltage 100 – 240 VAC, ± 10 % Wide-ranging capability
Line frequency 50 – 60 Hz, ± 5 %

Power consumption 250 VA Maximum
Ambient operating temperature 4 – 40 °C (41 – 104 °F)
Ambient non-operating temperature -40 – 70 °C (-4 – 158 °F)
Humidity < 95 %, at 25 – 40 °C (77 – 104 °F) Non-condensing
Operating Altitude Up to 2000 m (6500 ft)

Non-operating altitude Up to 4600 m (14950 ft) For storing the preparative
pump
Safety standards: IEC, CSA, UL Installation Category II, Pollution Degree 2
16
Installing the Pump
Installing the Pump
Unpacking the Preparative Pump
CA UT IO N In case you need to ship the preparative pump at a later date, it is useful to
keep the packing materials for a later shipment.
Damaged Packaging
Upon receipt of your preparative pump, inspect the shipping containers for
any signs of damage. If the containers or cushioning material are damaged,
save them until the contents have been checked for completeness and the
preparative pump has been mechanically and electrically checked. If the
shipping container or cushioning material is damaged, notify the carrier and
save the shipping material for the carrier’s inspection.
CA UT IO N If there are signs of damage to the preparative pump, please do not attempt to
install the preparative pump.
Delivery Checklist
Ensure all parts and materials have been delivered with the preparative
pump. The delivery checklist is shown in Table 2 - 5. To aid in parts
identification, please see chapter 5 “Parts and Materials”. There are two
different kits that can possibly be delivered with the pump:
1 A Basis kit (G1361-68708) is delivered with every isocratic configuration
or if the pump is the first pump in a system (isocratic configuration, order
No. G1361A).
2 A Gradient kit (G1361-68707) is delivered with every additional pump
that is added to a system’s first pump (gradient configuration, order No.
G1391A).
Please report missing or damaged parts to your local Agilent Technologies
sales and service office.
17
Installing the Pump
Installing the Pump
Table 2 First Preparative Pump (G1361A) Delivery Checklist
Description Quantity
Preparative Pump 1
Corrugated waste tubing 1
Power cable (local) 1
Manual (local) 1
Solvent cabinet 1
Basis kit (see Table 4) 1 with every FIRST (G1361A) pump of a system, only!
Table 3 Gradient Preparative Pump (G1391A) Delivery Checklist
Description Quantity
Preparative Pump 1
Corrugated waste tubing 1
Power cable (local) 1
Gradient kit (see Table 5) 1 with very ADD-ON (G1391A) pump of a system!
Table 4 Basis Kit Contents G1361-68708 (shipped with the first pump of a system)
Description Part Number Quantity
Bottle head assembly G1361-60002 1

Tube seal wash, 2m 0890-1764 1
Waste tube, reorder No. 5042-2461 1
Capillary 3, pump outlet to system G1361-67302 1
Bottle AMBER 2,0 l 9301-6341 1
Wrench open end 14-17 mm 8710-2435 1
Wrench open end 1/4-5/16" 8710-0510 2
Wrench open end 7/16-3/8” 8710-0972 1
18
Installing the Pump
Installing the Pump
Hex-key set 8710-0641 1

Pair of tweezers 5022-2195 1
Spare bottle inlet filter (frit) 3150-0944 1
Spare filter cup 3150-0942 1
Spare SST frit 2 µm 5022-2192 1
CAN cable, 1 m 5181-1519 1
Union preparative 5022-2133 1
Sanding paper no PN 2
ESD wrist-strap 9300-1408 1
19
Installing the Pump
Installing the Pump
Table 5 Gradient Kit Contents G1361-68707 (shipped with every additional pump in a
gradient system)

Tube seal wash 0890-1764 1
Waste tube 5042-2461 1
Bottle, clear 2,0 l 9301-6342 1
Capillary 5, pump A to T-union G1361-67304 1
Capillary 6, T-union to inlet of mixer G1361-67305 1
Female-T-union 0100-1016 1
Mixer 79835-87330 1
Capillary 7, pump B to T-union G1361-67306 1
Spare SST frit 2 µm 5022-2192 1
CAN cable, 0.5 m 5181-1516 1
20
Installing the Pump
Optimizing the Stack Configuration
If your preparative pump is part of a complete 1100 series system, you can
ensure optimum performance by limiting the configuration of the system
stack to one of the following configurations. The configurations shown on
the following pages are typical configurations. They optimize the systems
flow paths, ensuring minimum delay volumes.
NOTE For a detailed view of the flow connections refer to the section “Flow
connections” in chapter 1 of the reference manuals of the individual modules.
NOTE If a single stack of the 1100 modules becomes too high, e.g. if additional
modules like a G1327A ALS Thermostat or a second detector are added or if
your bench is too high, a two or more stack configuration may be a better
setup. Separate the stacks where appropriate and place the stacks beside each
other. This may require an additional (longer) capillary between the two
modules, where the stack has been separated. You should avoid any
configuration that adds unwanted delay volume between Sampler and
Column or dispersion volume between column and the detector(s).
The following sample setups are shown on the following pages:

1 A one Pump setup (isocratic system) with an Agilent 220 micro plate
sampler and one of the 1100 detectors.
2 Two pumps in a cluster acting as a binary Pump (gradient system) with an
Agilent 220 micro plate sampler and one of the 1100 detectors.
3 Two pumps in a cluster acting as a binary Pump with the Agilent 220 micro
plate sampler and the Agilent Quadrupol LC/MS detector.
21
Installing the Pump
Figure 1 Front View of the Recommended Stack Configuration for a one Pump setup
(isocratic system) with an Agilent 220 micro plate sampler and one of the 1100
Detectors
Solvent cabinet
Preparative pump
Micro plate
sampler Detector
NOTE For details on flow connections between the individual modules refer to
Chapter 1: “Flow connections” in the reference manuals of the individual
modules.
22
Installing the Pump
Figure 2 Rear View of the Recommended Stack Configuration for a one Pump setup
(isocratic system) with an Agilent 220 micro plate sampler and one of the 1100
Detectors
CAN Bus cable
Analog signal
to recorder
GPIB or LAN
to LC ChemStation
AC power
NOTE For details about control, remote and data cables and for flow connections to
and from the Agilent 220 MPS refer to Chapter 4: “Connecting the Agilent 220
micro plate sampler to 1100 Series LC” in the Agilent 220 micro plate sampler
reference manual (PN G2250-90002).
23
Installing the Pump
Figure 3 Front View of the Recommended Stack Configuration for a dual Pump setup (binary
gradient system) with an Agilent 220 micro plate sampler and one of the 1100
Detectors
Solvent cabinet
Preparative pumps
Micro plate
sampler Detector
modules.
24
Installing the Pump
Figure 4 Rear View of the Recommended Stack Configuration for a dual Pump setup (binary
gradient system) with an Agilent 220 micro plate sampler and one of the 1100
Detectors
CAN Bus cables
Analog signal
to recorder
GPIB or LAN
to LC ChemStation AC power
and from the Agilent 220 micro plate sampler refer to Chapter 4: “Connecting
the Agilent 220 micro plate sampler to 1100 Series LC” in the Agilent 220 micro
plate sampler reference manual (PN G2250-90002).
25
Installing the Pump
Figure 5 Front View of the Recommended Stack Configuration for a dual Pump setup (binary
gradient system) with the Agilent 220 micro plate sampler and the Agilent
Quadrupol LC/MS detector
Solvent cabinet
Preparative pump A
Agilent 220 micro LC/MS

plate sampler Detector
Preparative pump B
modules.
26
Installing the Pump
Figure 6 Rear View of the Recommended Stack Configuration for a dual Pump setup (binary
gradient system) with the Agilent 220 micro plate sampler and the Agilent
Quadrupol LC/MS detector
CAN bus cable
Remote start
cable
Network cable
LAN to LC
ChemStation
AC power
and from the Agilent Quadrupol LC-MS Detector refer to Chapter 2:
“Installation - Connecting the LC/MSD” in the Agilent 1100 Series LC/MSD
System Installation Manual (PN G1946-90002). For details on flow
connections between the individual modules refer to Chapter 1: “Flow
connections” in the reference manuals of the individual modules.
27
Installing the Pump
Installing the preparative pump
Preparations Locate bench space

Provide power connections
Unpack the pump
Parts required Pump
Power cord, for other cables see text below
ChemStation and/or Control module G1323A/B
1 Place the preparative pump on the bench in a horizontal position.

2 Ensure the power switch on the front of the preparative pump is OFF (switch
stands out).
3 At the rear of the preparative pump move the security lever to its maximum
right position.
Figure 7 Front View of the Preparative Pump
Status Lamp
Power Switch
Serial number
28
Installing the Pump
Figure 8 Power Connector and Safety Lever at Rear of the Preparative Pump
Safety lever
Power connector
4 Connect the power cable to the power connector at the rear of the module
(see Figure 8).
5 If required, connect additional interface and control cables to the preparative
pump (Figure 9). Refer to the documentation of the Agilent 1100 Series
control module or ChemStation for LC for more information.
NOTE In an Agilent 1100 Series system, the individual modules are connected by a
CAN cable. The Agilent 1100 control module can be connected to the CAN bus
on any of the modules in the system. The Agilent Chemstation can be
connected to the system by one GPIB cable at any of the modules that have a
GPIB port, however, it is recommended to connect the GPIB cable to the
detector. A second possibility to connect the 1100 system to the ChemStation
is via LAN, preferably at the detector. A LAN board can be delivered as an
option with one of the 1100 modules and installed in the BCD board slot. For
more information about connecting the control module or ChemStation refer
to the respective user manual.
regulations.
29
Installing the Pump
Figure 9 Preparative Pump Electrical Connections

CAN cable to previous module
Interface (BCD) board slot
8-bit configu-
ration switch
Remote
CAN-DC-out
RS232C CAN cable to next module
CAN-bus
• The CAN bus is a serial bus with high-speed data transfer. The two
connectors for the CAN bus are used for internal Agilent 1100 Series
module data transfer and synchronization.
• The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
common shut down, prepare, and so on.
• The RS-232 connector may be used to control the preparative pump from
a computer through an RS-232 connection, using appropriate software.
This connector needs to be activated by the 8-bit configuration switch
module in the upper right corner of the rear of the pump. The software
needs the appropriate drivers to support this communication. See your
software documentation for further information.
• The power input socket accepts a line voltage of 100 – 240 Volts AC ± 10 %
with a line frequency of 50 - 60 Hz. max. power consumption is 250 VA
(Volt-Amps). There is no voltage selector on your preparative pump
because the power supply has wide-ranging capability. There are no
externally accessible fuses. The security lever at the power input socket
prevents that the preparative pump cover is taken off when line power is
still connected.
• The interface (BCD) board slot is used for external contacts, BCD output
and for LAN communication.
• The CAN-DC-out provides 24 Volts DC power for external CAN devices
like a switch valve. Max. permanent power consumption is
100 mA/channel or 2 A for 2 sec.
30
Installing the Pump
Flow Connections for a single (isocratic) Preparative Pump
Flow Connections for a single (isocratic)

Preparative Pump
WAR NI N G When opening capillary or tube fittings solvents may leak out. Please
observe appropriate safety procedures (for example, goggles, safety
gloves and protective clothing) as described in the material handling
and safety data sheet supplied by the solvent vendor, especially when
toxic or hazardous solvents are used.
Preparation Pump is installed in the HPLC system

Parts required Other modules
Parts from basis kit (PN G1361-68708 see Table 4)
Wrench 1/4 - 5/16 inch for capillary connections
1 Remove the front cover by pressing the snap fasteners on both sides.
Figure 10 Removing the Front Cover
2 Place the solvent cabinet on top of the preparative pump.

3 Place the bottle into the solvent cabinet and place a bottle head assembly into
the bottle. Ground the solvent bottle!
31
Installing the Pump
WAR NI N G Danger of Explosion! Do not use metal bottles without appropriate

grounding! Pumping solvents at high flow rates generally results in
electrostatic charging of the solvents. To avoid electrostatic
discharge, which may result in the potential ignition of flammable
vapors or solvents, never use the pump without grounded metal
solvent bottles.
4 Connect the solvent tubes from the bottle head assemblies to the inlet
adapters of the pump. Fix the tubes in the clips of solvent cabinet and
preparative pump.
5 By holding a piece of sanding paper around the waste tubing connect it to the
electromagnetic purge valve (EMPV) and place it into your waste system.
6 If the preparative pump is not part of an Agilent 1100 System stack or placed
on the bottom of a stack, connect the corrugated waste tube to the waste
outlet of the pump leak handling system.
NOTE In order to guarantee an error-free handling of any leakage that may occur in
the system, all modules of a stack must be thoroughly aligned on top of each
other. Outstanding knobs at the rear of the top covers of the modules and
holes as counterparts at the rear of the bottom covers of the modules will help
to align the instruments nicely on top of each other by making them slide into
the correct position. This will then allow any possible spills to be routed safely
from one instrument to the next one with the help of the built in leak drainage
system. (Each module has a funnel to catch drops coming from the above
instrument, from there spills a lead through a waste drain tube into the leak
pan and out from there through an outlet on to the next module).
7 Connect the Outlet Capillary (G1361-67302) to the electromagnetic purge

valve (EMPV) and make the necessary connections the other side of the
capillary (normally a capillary to an injector or injection valve).
8 Install the seal wash tubings, as shown in Figure 11. You need a tubing from
your seal wash solvent bottle to the seal wash pump, one from there to the
pump head and one from the pump head to waste.
9 Install the front cover.
10 Press in the power switch to turn on the preparative pump.
32
Installing the Pump
Figure 11 Flow connections to the preparative pump
Outlet ball valves From solvent bottle

Tube clip
Leak funnel
Tubing: Seal wash to Leak drainage

waste
Inlet ball
valves EMPV out to
next module
From seal wash solvent bottle to seal wash pump EMPV to waste
Seal wash pump to pump head Waste outlet - corrugated waste tubing
33
Installing the Pump
Flow Connections for a dual (binary gradient) Preparative Pump
Flow Connections for a dual (binary

gradient) Preparative Pump
Preparation Pumps are installed in the HPLC system

Parts required Other modules
Parts from basis kit (PN G1361-68708 see Table 4)
Parts from gradient kit (PN G1361-68707 see Table 5)
Wrench 1/4 - 5/16 inch for capillary connections
1 Remove the front covers of both pumps by pressing the snap fasteners on both
sides.
Figure 12 Removing the Front Cover
2 Place the solvent cabinet on top of the preparative pumps.

3 Place the bottles into the solvent cabinet and place a bottle head assembly into
each bottle. Ground the solvent bottles!
34
Installing the Pump
WAR NI N G Danger of Explosion! Do not use metal bottles without appropriate

grounding! Pumping solvents at high flow rates generally results in
electrostatic charging of the solvents. To avoid electrostatic
discharge, which may result in the potential ignition of flammable
vapors or solvents, never use the pump without grounded metal
solvent bottles.
4 Connect the solvent tubes from the bottle head assemblies to the inlet
adapters of the pumps. Fix the tubes in the clips of solvent cabinet and
preparative pumps.
5 By holding a piece of sanding paper around the waste tubing connect it to the
electromagnetic purge valve (EMPV) of each pump and place it into your
waste system.
6 If one of the preparative pumps is placed on the bottom of a stack, connect the
corrugated waste tube to the waste outlet of the pump leak handling system.
NOTE In order to guarantee an error-free handling of any leakage that may occur in
the system, all modules of a stack must be thoroughly aligned on top of each
other. Outstanding knobs at the rear of the top covers of the modules and
holes as counterparts at the rear of the bottom covers of the modules will help
to align the instruments nicely on top of each other by making them slide into
the correct position. This will then allow any possible spills to be routed safely
from one instrument to the next one with the help of the built in leak drainage
system. (Each module has a funnel to catch drops coming from the above
instrument, from there spills a lead through a waste drain tube into the leak
pan and out from there through an outlet on to the next module).
7 Connect the long capillary (300 mm, G1361-67306) to the electromagnetic

purge valve (EMPV) of the upper pump and connect the female T-piece to the
other end of the capillary.
8 Connect the medium length capillary (140 mm, G1361-67304) to the
electromagnetic purge valve (EMPV) of the lower pump and connect the other
end of the capillary to the female T-piece.
9 Install the mixer (79835-87330) in the clips on the front panel of the bottom
pump.
10 Connect the capillary (G1361-67305) from the outlet of the female T-piece to
the mixer.
35
Installing the Pump
11 Install the outlet capillary (G1361-67302) to the mixer and make the necessary
connections the other side of the capillary (normally a capillary to an injector
or injection valve).
12 Install the seal wash tubings, as shown in Figure 13. You need - for each of the
pumps - a tubing from your seal wash solvent bottle to the seal wash pump,
one from there to the pump head and one from the pump head to waste.
13 Install the front covers.
14 Press in the power switch to turn on the preparative pumps.
36
Installing the Pump
Figure 13 Flow connections to the preparative pump

From solvent bottle A
Outlet ball valves
Tube clip
Leak funnel
Tubing: Seal wash to

waste Leak drainage
EMPV out to
Inlet ball bottom pump
valves G1361-67306
From seal wash
solvent bottle
From solvent bottle B
Female T-piece
Tubing: Seal wash to
waste G1361-67305
Mixer
G1361-67304
Seal wash pump to

pump head
From seal wash solvent bottle to seal wash pump EMPV to waste 0890-0615
Waste outlet - corrugated waste tubing Mixer out to next module G1361-67302
37
Installing the Pump
Get the System Ready for the First Analysis
Get the System Ready for the First

Analysis
When you are using the system for the first time it is recommended to prime
it to remove all the air and the possible contamination introduced in the flow
path during the installation.
NOTE The pump should never be used for priming empty tubings (never let the pump
run dry). Use a syringe to draw enough solvent for completely filling the
tubings to the pump inlet before continuing to prime with the pump.
Priming your preparative LC system with the pump

1 Set the flow rate to 5 ml/min and pump for 5 minutes with an open EMPV to
prime your new tubings and capillaries and to remove potential air bubbles or
contamination from the flow path.
2 Check your system for leak tightness with the help of the built in pressure test
(see “Pressure Test” on page 92).
3 Refer to Chapter 2 “Optimizing Performance” for optimizing your pump’s
performance.
4 Before starting an analysis pump until you receive a stable pressure signal
from your pump and until your detector baseline has stabilized (normally no
more than five minutes). Low %-ripple is a vital sign for a smoothly operating
pump and a stable system.
5 When the pumping system has been turned off for a certain time (for example,
overnight) repeat step 4.
38
Installing the Pump
Table 6 Choice of Priming Solvents for Different Purposes
Activity Solvent Comments
After an installation Isopropanol Best solvent to flush air out of

the system
When switching between Isopropanol Best solvent to flush air out of

reverse phase and normal the system
phase (both times)
After an installation Ethanol or Methanol Alternative to Isopropanol
(second choice) if no
Isopropanol is available
To clean the system when Bidistilled water Best solvent to re-dissolve
using buffers salts
After a solvent change Bidistilled water Best solvent to re-dissolve

salts
After the installation of Hexane + 5% Isopropanol Good wetting properties
normal phase seals (P/N
0905-1420)
39
Installing the Pump
40
2
2 Optimizing Performance
How to optimize the preparative pump to achieve

best performance
Optimizing Performance
Hints for Successful Use of the Preparative Pump
Hints for Successful Use of the

Preparative Pump
• Flush the pump extensively, when changing to a new solvent.

• The system pressure must be higher than 20 bar at the pump outlet for
optimum performance of the pump.
• Place the solvent cabinet with the solvent bottles always on top of the
preparative pump (or at a higher level).
• Prevent blocking of solvent inlet filters (never use the pump without
solvent inlet filters). Growth of algae should be avoided (see “Prevent
Blocking of Solvent Inlet Filters” on page 44).
• Regularly clean the filter cup and the filter frit installed in the multi
assembly. A back pressure greater than 10 bar, when pumping pure HPLC
grade water at a flow rate of 50 ml/min while the EMPV is open indicates
that one of the two filters is blocked or that the EMPV does not switch
(open) properly. Make sure to follow the correct procedures for cleaning
filters (see “Simple Repair Procedures” on page 114) and the EMPV (see
“EMPV Cleaning” on page 106). Always clean the pump’s filters, after
exchanging seals.
• Confirm that the pump and the rest of the system are completely leak tight
by performing the built in Leak-test (see “Leak Test” on page 99) and
Pressure-test (see “Pressure Test” on page 92), regularly.
• When using buffer solutions, flush the system with plenty of water to
remove all buffer solution from the entire system before switching it off or
before changing to an organic solvent.
• Always use the seal wash function, when working with buffers (see “How
to Configure the Seal Wash Function” on page 46).
• Check the pump plungers for scratches when changing the piston seals.
Scratched plungers will lead to micro leaks and will decrease the lifetime
of the seal.
• For the generation of gradients in systems with multiple pump setups
make sure that none of the pumps delivers less than a minimum flow rate
of 5 ml/min at any time during the gradient run, in order to achieve best
performance.
42
Solvent Information
Solvent Information
Always filter solvents through 0.4 µm filters, small particles can permanently
block the capillaries and valves. Avoid the use of the following
steel-corrosive solvents:
• Solutions of alkali halides and their respective acids (for example, lithium
iodide, potassium chloride, and so on).
• High concentrations of inorganic acids like sulfuric and nitric acid,
especially at higher temperatures (replace, if your chromatography
method allows, by phosphoric acid or phosphate buffer which are less
corrosive against stainless steel).
• Halogenated solvents or mixtures which form radicals and/or acids, for
example:
2CHCl3 + O2 → 2COCl2 + 2HCl
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the
stabilizing alcohol.
• Chromatographic grade ethers, which can contain peroxides (for example,
THF, dioxane, di-isopropylether) such ethers should be filtered through
dry aluminium oxide which adsorbs the peroxides.
• Solvents containing strong complexing agents (e.g. EDTA).
• Mixtures of carbon tetrachloride with 2-propanol or THF dissolve stainless
steel.
43
Prevent Blocking of Solvent Inlet Filters
Contaminated solvents or algae growth in the solvent bottle will reduce the
lifetime of the solvent filter and will influence the performance of the
preparative pump. This is especially true for aqueous solvents or phosphate
buffers (pH 4 to 7). The following suggestions will prolong lifetime of the
solvent filter and will maintain the performance of the preparative pump.
• Use sterile, if possible amber, solvent bottles to slow down algae growth.
• Filter solvents through filters or membranes that remove algae.
• Exchange solvents every two days or refilter.
• If the application permits add 0.0001 to 0.001 Molar sodium azide to the
solvent.
• Place a layer of argon on top of your solvent.
• Avoid exposure of the solvent bottles to direct sunlight.
• Filter HPLC grade (dry) Acetonitrile before use. The dryer the Acetonitrile,
the stronger the tendency to form polymers and therefore block the
system. Refilter at least every two days.
Checking the solvent inlet filters

The solvent filters are located on the low-pressure side of the preparative
pump. A blocked filter therefore does not affect the pressure readings of the
preparative pump. The pressure readings cannot be used to check whether
the filter is blocked or not. If the solvent cabinet is placed on top of the
preparative pump, the filter condition can be checked in the following way:
Remove the solvent inlet tube from the inlet valve of the pump and let the
tube hang down. If the filter is in good condition the solvent will freely run
out of the solvent tube (due to hydrostatic pressure). If the solvent filter is
partly blocked only little solvent will drip out of the solvent tube.
44
Cleaning the Solvent Filters

• Remove the blocked solvent filter from the bottle-head assembly and place
it in a beaker with concentrated nitric acid (65%) for one hour.
• Thoroughly flush the filter with bidistilled water (remove all nitric acid,
some columns can be damaged by nitric acid).
• Replace the filter.
NOTE Never use the system without solvent filters installed to avoid possible
blockages of the system.
45
How to Configure the Seal Wash Function
How to Configure the Seal Wash Function
Always use the seal wash function which is delivered with your pump.
NOTE The seal wash function cannot be operated permanently (danger of

overheating). Switch on the seal wash pump in regular intervals (normally for
30 s every 30 minutes) for a certain length of time. It is however important to
keep the back of the seals wet with the help of the seal wash function.
Water or Aqueous Buffer Solutions

We recommend a composition of 10% Isopropanol in HPLC grade water for
water or aqueous buffer solutions. With buffer solutions, the seal wash pump
should be configured such, that it is turned on for a duration of 30 s every
5 minutes (with a maximum of 30% cycle time). Shorten the intervals
between the pump turn-ons for highly concentrated buffer solutions (>0.1 M
buffer solutions).
Organic Solvents
For all organic non buffered solutions, we recommend to use the same
solvent for the seal wash as the one that is currently pumped by your
preparative pump. In this case, the seal wash pump has to be operated in
large intervals, only. Basically it is enough to keep the back of seals wet with
the help of the seal wash option.
NOTE Beware that the seal wash tubing is made of silicon. Never use any solvents
for the seal wash, that are not compatible with silicone.
46
How to Optimize the Compressibility Compensation Setting
How to Optimize the Compressibility

Compensation Setting
The compressibility compensation default settings are 75 × 10-6 /bar (best for
most aqueous solutions). Under normal conditions the default settings
reduce the pressure pulsation to values (below 2 % of system pressure for a
complete system with a preparative scale sized column installed) that will be
sufficient for most applications. If the compressibility values for the solvents
used differ from the default settings, it is recommended to change the
compressibility values accordingly. Compressibility settings can be
optimized by using the values for various solvents described in Table 7 on
page 48. If the solvent in use is not listed in the compressibility table, when
using premixed solvents and if the default settings are not sufficient for your
application the following procedure can be used to optimize the
compressibility settings:
NOTE Use the preparative pump with a flow rate of at least 20 ml/min.
1 Start the preparative pump with the adequate flow rate. The system pressure
should be between 50 and 150 bar
2 Before starting the optimization procedure, the flow must be stable. Before
starting the procedure, check the tightness of the system with the help of the
built in pressure test (see “Pressure Test” on page 92).
3 Verify the tightness of the pump itself (piston/seal combinations, valves, filters
fittings, etc.) with the help of the built in Leak Test (see “Leak Test” on page
99).
4 Your pump must be connected to a ChemStation or handheld controller, the
pressure and %-ripple can be monitored with one of these instruments.
5 Starting with a compressibility setting of 50 × 10-6 /bar increase the value in
steps of 10. The compressibility compensation setting that generates the
smallest pressure ripple is the optimum value for your solvent composition.
6 Repeat step 1 through step 5 for each preparative pump in a system with a
multiple pump setup.
47
How to Optimize the Compressibility Compensation Setting
Table 7 Solvent Compressibility
Solvent (pure) Compressibility (10-6/bar)
Acetone 126
Acetonitrile 115
Benzene 95
Carbon tetrachloride 110
Chloroform 100
Cyclohexane 118
Ethanol 114
Ethyl acetate 104
Heptane 120
Hexane 150
Isobutanol 100
Isopropanol 100
Methanol 120
1-Propanol 100
Toluene 87
THF 95
Water 46
48
3
3 Troubleshooting and
Test Functions
The preparative pump’s built-in troubleshooting

and test functions
Troubleshooting and Test
Functions
Status Indicators and Operation
This chapter describes the instrument’s built in troubleshooting and test

functions.
Status Indicators
The preparative pump is provided with two status indicators which indicate
the operational state (prerun, run, and error states) of the preparative pump.
The lower left indicates the power supply status, the upper right indicates the
preparative pump status.The status indicators provide a quick visual check of
the operation of the preparative pump (see “Status Indicators” on page 52).
Error Messages
In the event of an electronic, mechanical or hydraulic failure, the preparative
pump generates an error message in the user interface. The following pages
describe the meaning of these error messages. For each message, a short
description of the failure, a list of probable causes of the problem, and a list
of suggested actions to fix the problem are provided (see “Error Messages”
on page 55).
Pressure Test
The pressure test is a quick test designed to determine the pressure tightness
of the system. After exchanging flow path components (e.g., pump seals or
injection seal), use this test to verify the system is pressure tight up to 400 bar
(see “Pressure Test” on page 92).
Leak Test
The leak test is a diagnostic test designed to determine the pressure tightness
of the preparative pump. When a problem with the preparative pump is
suspected, use this test to help troubleshoot the preparative pump and its
pumping performance (see “Leak Test” on page 99).
50
Troubleshooting and Test Functions
EMPV Cleaning
Depending on the application, particles can sometimes be collected in the
EMPV valve. This cleaning procedure is designed to remove the particle
deposits. This procedure should always be performed when the EMPV is
suspected of being leaky, or contaminated with particles (see “EMPV
Cleaning” on page 106).
51
Status Indicators
Two status indicators are located on the front of the preparative pump. The
lower left one indicates the power supply status, the upper right one
indicates the instrument status.
Figure 14 Location of Status Indicators
Status indicator
Power supply indicator
52
Power Supply Indicator
Power Supply Indicator
The power supply indicator is integrated into the main power switch. When
the indicator is illuminated (green) the power is ON.
NOTE The power switch stays pressed in and a green indicator lamp in the power
switch is on when the preparative pump is turned on. When the line power
switch stands out and the green light is off, the preparative pump is turned off.
Instrument Status Indicator
The instrument status indicator on the top right corner of the preparative
pump’s front indicates one of four possible instrument conditions:
• When the status indicator is OFF (and power switch light is on), the
preparative pump is in a prerun condition, and is ready to begin an
analysis.
• A green status indicator, indicates the preparative pump is performing an
analysis (run mode).
• A yellow indicator indicates a not-ready condition. The preparative pump
is in a not-ready state when it is waiting for a specific condition to be
reached or completed (for example, immediately after changing a
setpoint), or while the purge valve is open or while a self-test procedure is
running.
• An error condition is indicated when the status indicator is red. An error
condition indicates the preparative pump has detected an internal problem
which affects correct operation of the instrument. Usually, an error
condition requires attention (for example, leak, defective internal
components). An error condition always interrupts the analysis.
• A flashing yellow status indicator indicates that the module is in its
resident mode. Call your local service provider for assistance upon
observing this error condition.
53
Instrument Status Indicator
• A flashing red status indicator indicates a severe error during the startup
procedure of the module. Call your local service provider for assistance
upon observing this error condition.
54
Error Messages
Error Messages
Error messages are displayed in the user interface when an electronic,

mechanical, or hydraulic (flow path) failure occurs which requires attention
before the analysis can be continued (for example, repair, frit exchange or
exchange of consumables required). In the event of such a failure, the red
status indicator at the front of the module is switched on, and an entry is
written into the instrument logbook.
55
Time-out
Time-out
The time-out threshold was exceeded.

Probable Causes • The analysis was completed successfully, and the time-out function
switched off the preparative pump as requested.
• A not-ready condition was present during a sequence or multiple-injection
run for a period longer than the time-out threshold.
Suggested Actions ❏ Check the logbook for the occurrence and source of a not-ready condition.
Restart the analysis where required.
56
Shutdown
Shutdown
An external instrument has generated a shut-down signal on the remote line.

The preparative pump continually monitors the remote input connectors for
status signals. A LOW signal input on pin 4 of the remote connector generates
the error message.
Probable Causes • Leak detected in another module with a CAN connection to the system.
• Leak detected in an external instrument with a remote connection to the
system.
• Shut-down in an external instrument with a remote connection to the
system.
Suggested Actions ❏ Fix the leak in the external instrument before restarting the preparative
pump.
❏ Check external instruments for a shut-down condition.
57
Remote Time-out
Remote Time-out
A not-ready condition is still present on the remote input.

When an analysis is started, the system expects all not-ready conditions (e.g.
a not-ready condition during detector balance) to switch to run conditions
within one minute of starting the analysis. If a not-ready condition is still
present on the remote line after one minute the error message is generated.
Probable Causes • Not-ready condition in one of the instruments connected to the remote
line.
• Defective remote cable.
• Defective components in the instrument showing the not-ready condition.
Suggested Actions ❏ Ensure the instrument showing the not-ready condition is installed
correctly, and is set up correctly for analysis.
❏ Exchange the remote cable.
❏ Check the instrument for defects (refer to the instrument’s reference
documentation).
58
Synchronization Lost
Synchronization Lost
During an analysis, the internal synchronization or communication between

one or more of the modules in the system has failed.
The system processors continually monitor the system configuration. If one
or more of the modules is no longer recognized as being connected to the
system, the error message is generated.
Probable Causes • CAN cable disconnected.
• Defective CAN cable.
• Defective main board in another module.
Suggested Actions ❏ Ensure all the CAN cables are connected correctly.
❏ Switch off the system. Restart the system, and determine which module or
modules are not recognized by the system.
❏ Ensure all CAN cables are installed correctly.
59
Leak
Leak
A leak was detected in the preparative pump.

The signals from the two temperature sensors (leak sensor and
board-mounted temperature-compensation sensor) are used by the leak
algorithm to determine whether a leak is present. When a leak occurs, the
leak sensor is cooled by the solvent. This changes the resistance of the leak
sensor which is sensed by the leak-sensor circuit on the PPM board.
Probable Causes • Loose fittings.
• Broken capillary.
• Loose or leaking inlet or outlet ball valve or EMPV.
• Defective pump seals.
Suggested Actions ❏ Ensure all fittings are tight.
❏ Exchange defective capillaries.
❏ Ensure pump components are seated correctly. If there are still signs of a
leak, exchange the appropriate seal (inlet or outlet ball valve or EMPV).
❏ Exchange the pump seals.
60
Leak Sensor Open
Leak Sensor Open
The leak sensor in the preparative pump has failed (open circuit).
The current through the leak sensor is dependent on temperature. A leak is
detected when solvent cools the leak sensor, causing the leak-sensor current
to change within defined limits. If the current falls outside the lower limit, the
error message is generated.
Probable Causes • Leak sensor not connected to the PPM board.
• Defective leak sensor.
• Leak sensor incorrectly routed, being pinched by a metal component.
Suggested Actions ❏ Ensure the leak sensor is connected correctly.
❏ Exchange the leak sensor.
61
Leak Sensor Short
Leak Sensor Short
The leak sensor in the preparative pump has failed (short circuit).
The current through the leak sensor is dependent on temperature. A leak is
detected when solvent cools the leak sensor, causing the leak-sensor current
to change within defined limits. If the current increases above the upper
limit, the error message is generated.
Probable Causes • Defective leak sensor.
• Leak sensor incorrectly routed, being pinched by a metal component.
Suggested Actions ❏ Exchange the leak sensor.
62
Compensation Sensor Open
Compensation Sensor Open
The ambient-compensation sensor (NTC) on the PPM board in the

preparative pump has failed (open circuit).
The resistance across the temperature compensation sensor (NTC) on the
PPM board is dependent on ambient temperature. The change in resistance is
used by the leak circuit to compensate for ambient temperature changes. If
the resistance across the sensor increases above the upper limit, the error
message is generated.
Probable Causes • Defective PPM board.
Suggested Actions ❏ Exchange the PPM board.
63
Compensation Sensor Short
Compensation Sensor Short
The ambient-compensation sensor (NTC) on the PPM board in the

preparative pump has failed (short circuit).
The resistance across the temperature compensation sensor (NTC) on the
PPM board is dependent on ambient temperature. The change in resistance is
used by the leak circuit to compensate for ambient temperature changes. If
the resistance across the sensor falls below the lower limit, the error message
is generated.
Probable Causes • Defective PPM board.
Suggested Actions ❏ Exchange the PPM board.
64
Fan Failed
Fan Failed
The cooling fan in the preparative pump has failed.

The hall sensor on the fan shaft is used by the PPM board to monitor the fan
speed. If the fan speed falls below a certain limit for a certain length of time,
the error message is generated.
Probable Causes • Fan cable disconnected.
• Defective fan.
• Defective PPM board.
• Improperly positioned cables or wires obstructing fan blades.
Suggested Actions ❏ Ensure the fan is connected correctly.
❏ Exchange fan.
❏ Exchange the PPM board.
❏ Ensure the fan is not mechanically blocked.
65
Open Cover
Open Cover
The top foam has been removed.

The sensor on the PPM board detects when the top foam is in place. If the
foam is removed, the pump is turned off within 4s for safety reasons, and the
error message is generated. If the pump is started without cover, this error
message is also generated and the pump is not turned on.
Probable Causes • The top foam was removed during operation or pump started without
cover.
• Foam not activating the sensor.
• Rear of the module is exposed to strong direct sunlight.
• Sensor defective.
Suggested Actions ❏ Replace the top foam.
❏ Ensure that the top foam is positioned correctly.
❏ Ensure that the rear of the module is not exposed to strong sunlight.
WAR NI N G Any adjustment, maintenance or repair of the instrument with the top
cover removed and with the power cord plugged in is strictly
forbidden.
WAR NI N G Disconnect the instrument from the line and unplug the power cord
before maintenance.
Do not remove instrument covers when operating. Before the instrument is

switched on, all protective earth terminals, extension cords,
auto-transformers, and devices connected to it must be connected to a
protective earth via a ground socket. Any interruption of the protective earth
grounding will cause a potential shock hazard that could result in serious
personal injury. Whenever it is likely that the protection has been impaired,
the instrument must be made inoperative and be secured against any
intended operation.
66
Pressure Above Upper Limit
Pressure Above Upper Limit
The system pressure has exceeded the upper pressure limit. This error
condition automatically results in the opening of the purge valve.
Probable Causes • Upper pressure limit set too low.
• Blockage in the flowpath (after the pressure sensor).
• Defective pressure sensor.
Suggested Actions ❏ Ensure the upper pressure limit is set to a value suitable for the analysis.
❏ Check for blockage in the flowpath.
❏ Exchange the Multi Assembly.
67
Pressure Below Lower Limit
Pressure Below Lower Limit
The system pressure has fallen below the lower pressure limit.
Probable Causes • Lower pressure limit set too high.
• Air bubbles in the mobile phase.
• Leak.
Suggested Actions ❏ Ensure the lower pressure limit is set to a value suitable for the analysis.
❏ Ensure bubble free flow. Purge the preparative pump.
❏ Ensure solvent inlet filters are not blocked.
❏ Inspect the pump head, capillaries and fittings for signs of a leak.
❏ Purge the preparative pump. Run a pressure test to determine whether the
seals or other pump components are defective.
❏ Exchange the Multi Assembly.
68
Pressure Sensor not Connected
Pressure Sensor not Connected
The pressure readings, read by the pump ADC (analog-digital converter), are
missing.
The ADC reads the pressure readings from the pressure sensor every 1ms. If
the readings are missing for longer than 10 seconds, the error message is
generated.
Probable Causes • Pressure sensor not connected (should be connected to P29 on the PPM
board) or connected to the seal wash cable connector (P23).
Suggested Actions ❏ Ensure the pressure sensor connector is clean, connected and seated
correctly.
❏ Exchange multi assembly.
69
Wrong Data from Pressure Sensor
Wrong Data from Pressure Sensor
The pressure sensor delivers readings within the range of -5 to -25 bar.
Probable Causes • Defective pressure sensor.
Suggested Actions ❏ Exchange the pressure sensor.
70
Motor Drive Temperature too High
Motor Drive Temperature too High
The temperature of the motor-drive circuits is too high.

The processor continually monitors the temperature of the drive circuits on
the PPM board. If excessive current is being drawn for long periods, the
temperature of the circuits increase. If the temperature exceeds the upper
limit of 95 ºC, the error message is generated.
Probable Causes • High friction (partial mechanical blockage) in the pump drive assembly.
• Partial blockage of the flowpath in front of the pressure sensor.
• Defective drive assembly.
Suggested Actions ❏ Ensure the capillaries and frits between the pump head and pressure
sensor (at the rear of the multi assembly) are free from blockage. Clean the
filters in the multi assembly.
❏ Ensure the outlet valve is not blocked.
❏ Remove the pump head assembly. Ensure there is no mechanical blockage
of the pump head assembly or pump drive assembly.
❏ Exchange defective drive assembly.
71
Motor Drive Fault
Motor Drive Fault
The motor drive chip has issued a fault signal. The current drawn by the
pump motor exceeded the maximum limit or under voltage lockout.
Blockages in the flowpath are usually detected by the pressure sensor, which
result in the preparative pump switching off when the upper pressure limit is
exceeded. If a blockage occurs before the pressure sensor (i.e. the pressure
increase cannot be detected by the pressure sensor), the preparative pump
will continue to pump. As pressure increases, the pump drive draws more
current. When the current reaches the maximum limit, the preparative pump
is switched off, and the error message is generated.
Probable Causes • Flow path blockage in front of the pressure sensor.
• Blocked outlet ball valve.
• High friction (partial mechanical blockage) in the drive assembly.
❏ Exchange the outlet ball valve.
of the pump head assembly or drive assembly.
❏ Exchange the drive assembly.
72
Motor Maximum Power Fault
Motor Maximum Power Fault
The motor control has determined that the set maximum for the motor power
was exceeded for longer than 1 second. To protect the circuitry from damage
this error event is issued in this case.
Blockages in the flowpath are usually detected by the pressure sensor in the
multi assembly, which results in the preparative pump switching off when the
upper pressure limit is exceeded. If a blockage occurs before the pressure
sensor (i.e. the pressure increase cannot be detected by the pressure sensor),
the preparative pump will continue to pump. As pressure increases, the pump
drive draws more current. When the current reaches the maximum limit, the
preparative pump is switched off, and the error message is generated.
Probable Causes • Flow path blockage in front of the pressure sensor.
• Blocked outlet ball valve.
• High friction (partial mechanical blockage) in the drive assembly.
❏ Exchange the outlet ball valve.
of the pump head assembly or drive assembly.
❏ Exchange the drive assembly.
73
Motor Direction
Motor Direction
The motor control has determined that the motor turns in opposite direction.
Probable Causes • Cables connected in wrong orientation after a repair.
Suggested Actions ❏ Ensure the cables are correctly connected (on the main board and at the
pump drive) and reconnect with new orientation, if necessary.
74
Pump Off While Analysis
Pump Off While Analysis
This error message is generated, if the pump was turned off, during an
analysis (Power Off or pump in a Not-Ready condition).
Probable Causes • Power Off or pump in a Not-Ready condition.
Suggested Actions ❏ Turn On the Pump.
❏ Eliminate the cause of the Not-Ready condition.
75
Index Missing
Index Missing
The pump motion control expects one index signal for every turn of the drive
wheel. The error message is generated, if this signal is not received within
one complete turn of the drive wheel.
Probable Causes • Defective or disconnected index cable.
• Defective Pump drive defective index board on pump drive.
• Defective main board.
Suggested Actions ❏ Ensure the connector connected and seated correctly.
❏ Exchange the pump drive assembly.
❏ Exchange the main board.
76
Position Error
Position Error
The motion control of the pump drive could not reach the desired condition
within a certain time frame. The deviation from the desired position
exceeded the error limit.
Probable Causes • Blocked flow path.
• Mechanical blockage of the pump drive assembly.
Suggested Actions ❏ Ensure that there is no blockage in the flow path before the pressure
sensor.
77
Spurious Index
Spurious Index
The pump motion control expects one (and only one) index signal for every
turn of the drive wheel. This error message is generated, if this signal is
received more often than within one complete turn of the drive wheel.
Probable Causes • Missing reflections to light switch because of contamination on drive
wheel.
• Defective Pump drive.
Suggested Actions ❏ Ensure the drive wheel is clean.
78
Encoder not Connected
Encoder not Connected
There is an encoder wheel mounted to the shaft of the pump motor, that gives
pulses to the motion control according to the rotation of the shaft. The error
message is generated if the short bridge on the connector is not sensed.
Probable Causes • Defective or disconnected pump encoder connector or cable.
Suggested Actions ❏ Ensure the connector is clean, and seated correctly.
❏ Exchange main board.
79
Purge Valve (EMPV) Fuse Blown
Purge Valve (EMPV) Fuse Blown
The electronic fuse has detected that the current through the purge valve was
too high.
Probable Causes • Defective purge valve.
Suggested Actions ❏ Exchange the drive of the EMPV.
80
Electro-Magnetic-Proportional-Valve (EMPV) Missing
Electro-Magnetic-Proportional-Valve
(EMPV) Missing
The EMPV in the preparative pump is missing or defective.

Probable Causes • Disconnected or defective cable.
• Defective purge valve.
Suggested Actions ❏ Ensure the connection cable is seated correctly.
❏ Exchange the drive of the EMPV.
❏ Exchange PPM board.
81
No Pump Head
No Pump Head
The pump-head RF-tag in the preparative pump was not found.

Probable Causes • Pump head not installed correctly (screws not secured, or pump head not
seated correctly).
• RF-tag on pump-head missing or defective.
• Wrong pump-head connected.
Suggested Actions ❏ Install the pump head correctly. Ensure nothing (e.g. capillary) is trapped
between the pump head and body.
❏ Install missing or replace defective RF-tag.
❏ Install the original Agilent pump-head with RF-tag.
❏ Exchange PPM board.
82
Wait for Volume Timed Out
Wait for Volume Timed Out
The wait time limit until a given volume has been pumped, has been
exceeded.
Probable Causes • Wrong limit set for the time limit.
• Flow setting too low.
Suggested Actions ❏ Set limit accordingly.
❏ Set flow accordingly.
83
Wait for Pressure Timed Out
Wait for Pressure Timed Out
The limit, for waiting until a given pressure is reached, has been exceeded.
Probable Causes • Wrong limit set for the time limit.
• Leak.
Suggested Actions ❏ Set limit accordingly.
❏ Check for leaks with the help of the built in pressure or leak test and
correct them, where necessary.
84
Composition Cluster Partner Lost
Composition Cluster Partner Lost
Communication to a composition cluster partner was interrupted.

In a gradient system, two (or more) isocratic preparative pumps can be
linked together in a cluster acting as one gradient pump. The error message is
generated, if the communication between the partners of a cluster is
interrupted.
Probable Causes • Composition cluster partner was turned off.
• CAN-cable to composition cluster partner disconnected.
• Defective CAN-bus.
Suggested Actions ❏ Turn on composition cluster partner.
❏ Reconnect or replace CAN-cable.
❏ Replace PPM main board.
85
Solvent Counter Zero
Solvent Counter Zero
The volume counter for the bottle filling reached zero.

Probable Causes • Value for bottle filling set incorrectly.
• Solvent bottle empty.
Suggested Actions ❏ Reset bottle filling to the correct value.
❏ Refill solvent bottle.
86
No Motor Motion
No Motor Motion
No motor rotation feedback.

Probable Causes • Defective or disconnected index cable.
• Encoder cable not connected at the encoder.
• Motor defective or not connected.
Suggested Actions ❏ Ensure the connector connected and seated correctly.
❏ Ensure the encoder cable is connected and seated correctly at the
encoder.
87
Seal Wash Current Low
Seal Wash Current Low
The current sensing of the seal wash motor was lower than expected.
Probable Causes • Defective or disconnected seal wash pump motor.
Suggested Actions ❏ Ensure the cable is correctly connected.
❏ Exchange the seal wash pump motor.
88
Seal Wash
Seal Wash
This error message is generated, if the pressure sensor is installed to the

connector on the main board, that should hold the seal wash motor cable.
WAR NI N G If the seal wash pump is turned on, while the pressure sensor is
connected to the main board port that should have the seal wash pump
installed, this can result in a defective pressure sensor (requires
exchange of complete multi assembly).
Probable Causes • Pressure sensor and seal wash pump connector were connected to the
wrong position on the main board (identical connectors allow to plug into
the wrong positions by mistake)
Suggested Actions ❏ Switch the connectors of the seal wash pump cable and the pressure
sensor to meet the correct positions (P23 for seal wash cable, P29 for the
pressure sensor cable from the multi assembly).
89
Wait for Encoder Position Timed Out
Wait for Encoder Position Timed Out
Encoder position was not reached within specified time.

Probable Causes • Flow too low.
• Pump Off.
Suggested Actions ❏ Recalculate flow or wait time, to allow to reach encoder position before
encoder position time-out.
❏ Turn On pump.
90
24V Supply for External Devices Low
24V Supply for External Devices Low
Voltage sensing of the 24 Volts CAN outlet shows low voltage, probably fuse
has blown.
Probable Causes • Excessive current has been drawn by an external device connected to the
external 24 CAN-DC out.
Suggested Actions ❏ Ensure, that any device, connected to the external 24 CAN-DC out, does
not draw more than 100 mA as a permanent current or a maximum of 2 A
for no longer than 2 seconds. The error condition will be reset
automatically, when the excessive current is not drawn from the external
24 CAN-DC out port any more.
91
Pressure Test
Pressure Test
Description
The pressure test is a quick, built-in test designed to demonstrate the
pressure-tightness of the system. The test should be used when problems
with leaks are suspected, or after maintenance of flow-path components
(e.g., pump seals, injection seal) to prove pressure tightness up to 350 bar.
The test involves monitoring the flow rate, required to keep a constant
pressure of 350 bar, as the preparative pump runs through a predefined
pumping sequence. The resulting flow rate provides information about the
pressure tightness of the system.
The last connection on the high pressure side of the system (usually the
fitting that connects to the inlet of the column) is blocked by a blank nut, and
then the test is run using isopropyl alcohol (IPA), while monitoring the flow
rate. The screen for the pressure test, performed with the ChemStation, is
shown in Figure 15.
92
Pressure Test
Figure 15 Pressure-Test Screen in the ChemStation
Step 1 After the preparation of the system by thoroughly flushing the entire system
with Isopropanol and removing all contaminations and potential air bubbles,
releasing the pressure in the system and positioning the blank nut, the test
begins with an automated flushing of the pump at a flow rate of 25 ml/min
with an open EMPV.
Step 2 The EMPV is closed and next, piston A begins pumping solvent with a flow
rate of 2 ml/min until reaching a pressure of 150 bar, then the pump delivers
at a flow rate of 0.2 ml/min until reaching a pressure of 250 bar, then the
pump delivers at a flow rate of 0.1 ml/min until reaching a pressure of
345 bar, then the pump switches into its “Keep pressure mode”.
NOTE To test the pressure tightness of all individual components in the flow path of
the pump itself use the leak test, see “Leak Test” on page 99.
Step 3 When the system pressure reaches 350 bar, the preparative pump switches to
its “Keep-pressure-mode”. The flow rate to compensate for leaks should from
this point onwards be no more than 10 µl/minute. The results of the test are
automatically evaluated and updated. So it is possible to tighten some fittings
in order to remove leaks, while monitoring the flow rate, required to keep the
constant pressure. Figure 16 shows the result of the pressure test.
93
Pressure Test
Figure 16 Result of the Pressure Test
Positioning the Blank Nut

If a specific component is suspected of causing a system leak, place the blank
nut immediately before the suspected component, then run the pressure test
again.If the test passes, the defective component is located after the blank
nut. Confirm the diagnosis by placing the blank nut immediately after the
suspected component. The diagnosis is confirmed if the test fails.
94
Running the Pressure Test
Tools required Wrench 1/4 inch.

Parts and materials Blank nut, 01080-83202
required Isopropanol, 500 ml
NOTE Make absolutely sure that all parts of the flow path that are part of the
test are very thoroughly flushed with IPA before starting to pressurize
the system! Any trace of other solvents or the smallest air bubble
inside the flow path definitely will cause the test to fail!
Running the Test from the ChemStation

1 Select the “Pressure Test” from the preparative pump’s tests selection box in
the ChemStation’s Diagnosis screen.
2 Start the test and follow the instructions.
3 After having received the first result for the test, the value for the flow rate
required to keep the system at a constant pressure, is permanently updated
automatically. Work on the system, until you have identified the leaky part and
removed the leak.
4 Finishing the test will release the pressure in the system.
5 Remove the blanking nut after finishing the test and reconnect all capillaries.
Running the Test from the Control Module

1 Select the ‘Pressure Test’ from the preparative pump’s tests selection box in
the Control Modules’s Test screen. (From the ‘Analysis’ screen press ‘F5’ and
select the ‘System’ screen. In the ‘System’ screen press ‘F3’ and select the
‘Prep pump’).
2 Start the test by pressing ‘Execute’ and follow the instructions.
3 After having received the first result for the test, the value for the flow rate
required to keep the system at a constant pressure, is permanently updated
automatically. Work on the system, until you have identified the leaky part and
removed the leak.
95
4 Finishing the test will release the pressure in the system.

5 Remove the blanking nut after finishing the test and reconnect all capillaries.
96
Evaluating the Results
The sum of all leaks between the pump and the blank nut will be indicated by
flow rate of >10 µl/minute to keep the pressure constant. Note that small
leaks may cause the test to fail, but solvent may not be seen leaking from a
module.
NOTE Please notice the difference between an error in the test and a failure of the
test! An error means that during the operation of the test there was an
abnormal termination. If a test failed, this means that the results of the test
where not within the specified limits.
If the pressure test fails:

• Ensure all fittings between the pump and the blank nut are tight. Watch any
effects on the flow rate to keep the pressure constant, while tightening the
fittings.
NOTE Do not overtighten fittings! After the first major increase of resistance when
tightening a previously loose fitting, the fitting should not be tightened any
further than an additional 1/8 to 1/4 wrench turn.
NOTE Often it is only a damaged blank nut itself (poorly shaped from overtightening)
that causes a failure of the test. Before investigating on any other possible
sources of failure make sure that the blank nut you are using is in good
condition and properly tightened!
• If the test fails again, insert the blank nut at the outlet of the previous
module in the stack and repeat the pressure test. Exclude each module one
by one to determine which module is leaking.
• If the pump is determined to be the source of the leak, run the EMPV
cleaning procedure, repeat the pressure test and if the test fails again, run
the leak test.
• Large internal leaks at the EMPV can easily be identified by observing a
flow out through the waste tubing of the EMPV, while the EMPV should be
closed.
97
Potential Causes of Pressure Test Failure

After isolating and fixing the cause of the leak, repeat the pressure test to
confirm the system is pressure tight.
Potential Cause Pump Corrective Action
Loose or leaky fitting. Tighten the fitting or exchange the capillary.

Untight EMPV Run the EMPV cleaning procedure.
Damaged pump seals or plungers. Run the leak test to confirm the leak.
Potential Cause Autosampler Corrective Action
Loose or leaky fitting. Tighten or exchange the fitting or capillary.

Needle seat. Exchange the needle seat.
Rotor seal (injection valve). Exchange the rotor seal.
Damaged metering seal or plunger. Exchange the metering seal. Check the
plunger for scratches. Exchange the plunger if
required.
98
Leak Test
Leak Test
Description
The leak test is a quick, built-in test designed to demonstrate the
pressure-tightness of the pump. The test should be used when problems with
leaks are suspected, or after maintenance of flow-path components (e.g.,
pump seals, filters, EMPV or capillaries) to prove pressure tightness up to
350 bar. The test involves monitoring the pump drive steps needed to build up
a pressure of 350 bar and the flow rate, required to keep a constant pressure
of 350 bar, as the preparative pump runs through a predefined pumping
sequence. The resulting flow rate provides information about the pressure
tightness of the pump. Each step is separately performed and evaluated
separately for piston 1 and piston 2.
The outlet of the EMPV is blocked by a blank nut, and then the test is run
using isopropyl alcohol (IPA), while monitoring the flow rate. The screen for
the leak test, performed with the ChemStation, is shown in Figure 15.
99
Leak Test
Figure 17 Leak-Test Screen in the ChemStation
Step 1 After the preparation of the pump by thoroughly flushing the pump with
Isopropanol and removing all contaminations and potential air bubbles,
releasing the pressure in the system and positioning the blank nut, the test
begins with an automated flushing of the pump at a flow rate of 25 ml/min
with an open EMPV.
Step 2 The EMPV is closed and next, piston 1 begins pumping solvent with a flow
rate of 2 ml/min until reaching a pressure of 150 bar, then the pump delivers
at a flow rate of 0.2 ml/min until reaching a pressure of 250 bar, then the
pump delivers at a flow rate of 0.1 ml/min until reaching a pressure of
345 bar, then the pump switches into its “Keep pressure mode”.
The result of the encoder value position shows how many metering steps
were needed to build up the pressure of 350 bar for each individual piston.
For piston 1 an encoder value position from 3500 - 16000 is allowed, for
piston 2 a value from 19500 - 32000 is allowed. Any failure to pressurize the
system within the specified ranges normally indicates an air bubble in the
pump.
Step 3 The flow rate to compensate for leaks should from this point onwards be no
more than 10 µl/minute. The results of the test are automatically evaluated.
Step 4 Steps 2 and three are automatically repeated for piston 2
100
Leak Test
Figure 18 Leak Test Results
101
Running the Leak Test
Running the Leak Test
Tools required Wrench 1/4 inch

required Isopropanol, 500ml
NOTE Make absolutely sure that all parts of the flow path that are part of the
test are very thoroughly flushed with IPA before starting to pressurize
the pump! Any trace of other solvents or the smallest air bubble inside
the flow path definitely will cause the test to fail!
NOTE It is recommended to run the EMPV cleaning procedure (see “EMPV Cleaning”
on page 106) prior to running the Leak test, to remove any potential deposits
from the EMPV valve.
The slopes and plateaus are evaluated automatically. “Evaluating the Results”
on page 103 describes the evaluation and interpretation of the leak test
results.
Running the Test from the ChemStation

1 Select the leak test from the test selection box in the Diagnosis screen.
2 Start the test and follow the instructions.
Running the Test from the Control Module

1 Select the ‘Leak Test’ from the preparative pump’s tests selection box in the
Control Modules’s Test screen. (From the ‘Analysis’ screen press ‘F5’ and
select the ‘System’ screen. In the ‘System’ screen press ‘F3’ and select the
‘Prep pump’).
2 Start the test by pressing ‘Execute’ and follow the instructions.
102
Defective or leaky components in the pump’s flow path lead to changes in the
leak-test pressure plot. Typical failure modes are described below.
Figure 19 Leak Test Pressure Plot
NOTE Please notice the difference between an error in the test and a failure of the
test! An error means that during the operation of the test there was an
abnormal termination. If a test failed, this means that the results of the test
where not within the specified limits.
NOTE Often it is only a damaged blank nut itself (poorly shaped from overtightening)
that causes a failure of the test. Before investigating on any other possible
sources of failure make sure that the blank nut you are using is in good
condition and properly tightened!
103
Error Limits Exceeded for One or Both of the Encoder Values and One
or Both of the Maximum allowed Flow Rates in the Constant Pressure
Mode
Potential Cause Corrective Action
Air bubble. Flush pump thoroughly.

Loose or leaky fittings. Ensure all fittings are tight, or exchange
capillary.
Large leaks (visible) at the pump seals. Exchange the pump seals.
Large leaks (visible) at valve, outlet valve, Ensure the leaky components are installed
filters, multi assembly, capillaries or EMPV. tightly. Exchange the component if required.
Run the EMPV cleaning procedure.
Maximum Allowed Flow Rate Exceeded for Both Pistons (1 and 2)
Wrong solvent. Use Isopropyl alcohol only.

Loose or leaky fittings. Ensure all fittings are tight, or exchange
capillary.
Contaminated EMPV. Run the EMPV cleaning procedure.
Loose pump head screws. Ensure the pump head screws are tight.
Large leaks (visible) at the pump inlet/outlet Tighten or exchange the pump inlet/outlet
valves. valves.
Large leaks (visible) at filters, multi assembly, Ensure the leaky components are installed
capillaries or EMPV. tightly. Exchange the component if required.
Run the EMPV cleaning procedure.
Leaky pressure sensor. Exchange the multi assembly.
Leaking seal or scratched plungers of channel Exchange the pump seals in both channels.
1 or channel 2. Check the plungers for scratches. Exchange if
scratched.
104
Maximum Allowed Flow Rate exceeded for Piston 1
Externally leaking inlet or outlet valve in Ensure the valves are installed correctly.
channel 1. Tighten the valves.
Internally leaking inlet valve in channel 1 Clean or replace the inlet valve in channel 1.
(back flow).
Internally leaking outlet valve in channel 2 Clean or replace the outlet valve in channel 2.
(back flow).
Leaking seal or scratched plunger in channel Exchange the pump seals in channel 1. Check
1. the plunger for scratches. Exchange if
scratched.
Loose or leaky fitting or capillary or fitting in Tighten fitting or replace capillary if
channel 1. necessary in channel 1.
Maximum Allowed Flow Rate Exceeded for Piston 2
Externally leaking inlet or outlet valve in Ensure the valves are installed correctly.
channel 2. Tighten the valves.
Internally leaking inlet valve in channel 2 Clean or replace the inlet valve in channel 2.
(back flow).
Internally leaking outlet valve in channel 1 Clean or replace the outlet valve in channel 1.
(back flow).
Leaking seal or scratched plunger in channel Exchange the pump seals in channel 2. Check
2. the plunger for scratches. Exchange if
scratched.
Loose or leaky fitting or capillary or fitting in Tighten fitting or replace capillary if
channel 2. necessary in channel 2.
105
EMPV Cleaning
EMPV Cleaning
Tools required Wrench7/16 inch.

required
Depending on the application, particles can sometimes be collected in the

EMPV valve. This fast cleaning routine is designed to remove such particle
deposits. The routine should always be performed when the EMPV is
suspected of being leaky, or contaminated with particles.
NOTE Large internal leaks at the EMPV can easily be identified by observing a flow
out through the waste tubing of the EMPV, while the EMPV should be closed.
The EMPV outlet is plugged with a blank nut (01080-83202). After a short
flushing routine the EMPV is closed and the pressure is increased to
approximately 350 bar. Then the EMPV is opened and the pressure is
released very fast. The procedure is repeated 5 times in a sequence.
Figure shows, the ChemStation’s screen for the procedure:
Figure 20 Cleaning the EMPV with the ChemStation’s built in procedure
106
EMPV Cleaning
1 Plug the pump outlet with a blank nut (01080-83202) at EMPV outlet.
2 Execute procedure (In the ChemStation’s Diagnosis view select ‘Tests’ and
choose the pump - EMPV Cleaning. In the Control Module go to System
Screen (F5, choose ‘System’)- Tests (F3) - select the pump and the EMPV
Cleaning procedure.).
NOTE This procedure can also be performed with the Control module by manually
entering a flow rate that pressurizes the pump that is blocked with a blanking
nut (01080-83202) at the EMPV outlet to 350 bar. Lower the flow rate with
increasing pressure. As the pressure approaches 350 bar, the pressure should
be released suddenly by turning on the EMPV. This procedure should be
repeated 5 times. Run a leak test (see “Leak Test” on page 99 after having
performed the cleaning procedure, to ensure tightness of the EMPV.
3 For better removal of any left-over particles, it will help to clean the ball of
the valve with an additional procedure, if the previous step did not help:
Using a 7/16” wrench remove deposits by turning the holding screw of the
EMPV body back and forth about 1/4 wrench turn several times.
Figure 21 Removing Deposits from the EMPV with the Help of a Wrench:
4 Remove the blank nut.

5 Reconnect all capillaries.
107
EMPV Cleaning
108
4
4 Repairing the Pump
Instructions on simple, routine repair procedures

as well as more extensive repairs requiring
exchange of internal parts
Repairing the Pump
Simple Repairs
The preparative pump is designed for easy repair. The most frequent repairs
such as plunger seal change and filter change can be done from the front of
the preparative pump with the preparative pump in place in the system stack.
These repairs are described in “Simple Repair Procedures” on page 114.
Exchanging Internal Parts

Some repairs may require exchange of defective internal parts (see
“Exchanging Internal Parts” on page 132). Exchange of these parts requires
removing the preparative pump from the stack, removing the covers, and
disassembling the preparative pump. The security lever at the power input
socket prevents that the pump cover is taken off when line power is still
connected.
WAR NI N G The following procedures require opening the main cover of the
preparative pump. Always ensure the preparative pump is
disconnected from the line power when the main cover is removed. The
security lever at the power input socket prevents that the pump cover
is taken off when line power is still connected. To prevent personal
injury, the power cable must be removed from the preparative pump
before opening the pump cover. Do not connect the power cable to the
preparative pump while the covers are removed.
110
Repairing the Pump
Simple Repairs
WAR NI N G To disconnect the preparative pump from line, unplug the power cord.
The power supply still uses some power, even if the switch on the front
panel is turned off.
WAR NI N G NEVER remove the cover of the power supply. Charged capacitor
devices may still contain voltages hazardous for the operator’s health,
even if the pump is NOT connected to line power. The power supply
does not contain any user serviceable parts.
NOTE The electronics of the preparative pump will not allow operation of the
preparative pump when the top cover and the top foam are removed. A safety
light switch on the main board will inhibit the operation of the preparative
pump. Always operate the preparative pump with the top foam and top covers
in place.
CA UT IO N Electronic boards and components are sensitive to electrostatic discharge

(ESD). In order to prevent damage always use an ESD protection (for
example, the ESD wrist strap from the accessory kit) when handling
electronic boards and components.
111
Repairing the Pump
Using the ESD Strap
Using the ESD Strap
Electronic boards are sensitive to electrostatic discharge (ESD). In order to

prevent damage, always use an ESD strap supplied in the standard accessory
kit (see “Preparative Pump Basis Kit” on page 169) when handling electronic
boards and components.
Using the ESD Strap

1 Unwrap the first two folds of the band and wrap the exposed adhesive side
firmly around your wrist.
2 Unroll the rest of the band and peel the liner from the copper foil at the
opposite end.
3 Attach the copper foil to a convenient and exposed electrical ground.
Figure 22 Using the ESD Strap
Cleaning the Preparative Pump

The preparative pump case should be kept clean. Cleaning should be done
with a soft cloth slightly dampened with water or a solution of water and a
mild detergent. Do not use an excessively damp cloth that liquid can drip into
WAR NI N G Do not let liquid drip into the preparative pump. It could cause shock
hazard and it could damage the preparative pump.
112
Repairing the Pump
Overview
Overview
Figure 23 shows the main assemblies of the preparative pump. The pump
heads and its parts do require normal maintenance (for example, seal
exchange) and can be accessed from the front (simple repairs). Replacing
internal parts will require to remove the module from its stack and to open
the top cover.
Figure 23 Overview of Repair Procedures

Pump drive, see page 144 Power supply, see
page 145
PPM board, see

page 138
Fan, see page 143

Outlet valves, see page 115
Pump head, see page 125
Inlet valves, see

page 115 Electromagnetic
purge valve, EMPV,
see page 121
Leak sensor, see page 146 Seal wash pump,
Multi assembly with filters and see page 124
pressure sensor (at the rear), see
page 117 , page 119 , page 136
113
Repairing the Pump
Simple Repair Procedures
Simple Repair Procedures
The procedures described in this section can be done with the preparative
pump in place in the system stack.
Table 8 Simple Repair Procedures
Procedure Symptom Potential causes

“Exchanging the Inlet/Outlet Ball Valves” on If leaking or not External/internal leak. Pressure ripple unstable, run
page 115 opening/closing properly leak test for verification
“Exchanging the Filter Cup” on page 117 Excessive back pressure After replacing pump seals,
with open EMPV, unstable
contaminated filter
pressure
“Exchanging the Filter Frit” on page 119 Excessive back pressure After replacing filter cup,
with open EMPV, unstable
particles on filter frit
pressure
“Exchanging the Complete EMPV or just the Valve internally/externally Deposits on valve ball or defective EMPV
Valve” on page 121 leaking
Intermittent pressure drops
“Exchanging the Seal Wash Pump Cassette” on Seal wash pump not If mechanically defect
page 124 delivering flow
Seal wash pump leaking
“Removing and Disassembling the Pump Head Leaks, unstable flow or If seal life time is shorter than normally expected —
Assembly” on page 125 pressure check plungers for scratches while changing the
seals
“Reassembling the Pump Head Assembly” on After pumphead repair
page 128
114
Repairing the Pump
Exchanging the Inlet/Outlet Ball Valves
When required If internally / externally leaking (back flow)

Tools required Wrench 17 mm
Wrench 1/4 inch
Material Valve assembly, PN G1361-60012
Removing an Inlet Ball Valve (Bottom of the Pump Head)
NOTE The inlet valves do not necessarily have to be replaced in pairs. Use the built
in Leak test (see “Leak Test” on page 99) to identify the valve, that is not
working properly.
1 Disconnect the solvent inlet tube at the inlet valve.

2 Using a 17-mm wrench loosen the inlet valve and remove the valve adapter,
including the valve assembly from the pump head.
Figure 24 Ball Valve Parts
Valve assembly
Valve adapter
115
Repairing the Pump
Removing an Outlet Ball Valve (Top of the Pump Head)
NOTE The outlet valves do not necessarily have to be replaced in pairs. Use the built
in Leak test (see “Leak Test” on page 99) to identify the valve, that is not
working properly.
1 Disconnect the solvent outlet capillary at the valve adapter, with a 1/4 inch
wrench. Beware of leaking solvents.
2 Using a 17-mm wrench loosen the outlet valve adapter and remove the valve
adapter from pump head.
3 Lift the valve adapter out of the housing in the pumphead.
4 With a pair of tweezers carefully remove valve assembly.
Replacing an Inlet/Outlet Valve

1 Before inserting the new valve assembly, clean the valve adapter by placing it
into an ultrasonic bath, if it shows visible signs of contamination. Clean the
valve housing in the pumphead by filling a syringe with alcohol and flushing
the housing thoroughly.
2 Insert a new valve assembly into the valve adapter. The arrow on Figure 24
indicates the flow direction. Make sure the valve assembly is fully inserted
into the valve adapter.
NOTE The side of the valve assembly that has the two holes must always face in flow
direction!
3 Insert the valve adapter with the new valve assembly into the housing and
screw tight.
4 Reconnect all capillaries and tubings.
NOTE Don’t reverse inlet and outlet valve adapters. The outlet valve adapters have a
smaller hole for fitting of the SST outlet capillary. The inlet adapters are
designed for the finger tight fittings of the inlet tubing.
116
Repairing the Pump
Exchanging the Filter Cup
When required After the pump seals have been exchanged

When the back pressure exceeds 10 bar pumping 50 ml/min of pure water with open purge
valve.
Tools required Wrench 17 mm
Wash bottle containing isopropanol
Parts required Filter cup, PN 3150-0942
NOTE Whenever replacing the filters on the multi assembly is required because of a
pump seal change or because excessive back pressure indicates the need for
a replacement, make sure to replace the filter cup first and then the filter frit
(see“Exchanging the Filter Frit” on page 119), after having pumped another
200 ml of solvent.
NOTE It is not possible to clean a contaminated filter cup in an ultrasonic bath

because any contamination of the filter is normally permanent.
1 Using a 17 mm wrench loosen filter screw on the multi assembly.

2 Carefully pull out filter screw and filter cup.
3 Remove the filter cup from the screw by holding the screw and filter and
pulling them apart.
NOTE Generally, all components which are in direct contact with solvents should be
handled wearing gloves.
117
Repairing the Pump
Figure 25 Exchanging the Filter Cup
Filter Screw
Filter Cup
4 Before reinserting the new filter screw into the multi assembly, wash the filter
housing thoroughly with a wash bottle, containing Isopropanol, to remove any
contamination.
5 Install a new filter into the filter screw.
6 Reinsert the filter and filter screw and turn until finger tight, then another 1/8
turn using a 17 mm wrench.
NOTE Do not overtighten the filter screw. Excessive force on the filter screw doesn’t
help in case of a leak.
7 Pump about 200 ml of solvent before replacing the filter frit installed at the
multi assembly: the filter frit between the outlet of the multi assembly and the
EMPV (see “Exchanging the Filter Frit” on page 119).
118
Repairing the Pump
Exchanging the Filter Frit
When required When the back pressure exceeds 10 bar pumping 50 ml/min of pure water with open purge
valve. After 200 ml solvent have been pumped through the pump after a filter cup exchange.
Wrench 1/4 inch
Hex-key 4 mm
Wash bottle containing Isopropanol
Parts required Filter frit 2µm, PN 5022-2192
NOTE Whenever replacing the filters on the multi assembly is required because of a
pump seal change or because excessive back pressure indicates the need for
a replacement, make sure to replace the filter cup first (see “Exchanging the
Filter Cup” on page 117) and then the filter frit, after having pumped another
200 ml of solvent.
Changing the Filter Frit

1 Using a 1/4 inch wrench disconnect the capillary between multi assembly and
EMPV.
2 Insert a hex-key in to hole in the multi assembly. Hold the hex-key firmly and
unscrew the locknut using the 7/16 inch wrench.
119
Repairing the Pump
Figure 26 Changing the Filter Frit
Filter frit
Hole
Filter holder
3 Pull out the filter holder and filter.

4 Flush the filter port and filter holder using a wash bottle containing
isopropanol.
5 Install a new filter frit into the filter holder with the collar of the filter frit
facing towards the multi assembly. Reconnect the filter holder into
multi-assembly. Finger tighten screw and then turn 1/8 turn using 7/16 inch
wrench and hex-key.
Figure 27 Exchanging the Filter frit
Filter holder
Filter frit
6 Reconnect capillary between multi assembly and EMPV using a 1/4 inch
wrench.
120
Repairing the Pump
Exchanging the Complete EMPV or just the Valve
Exchanging the Complete EMPV or just

the Valve
When required If valve internally leaking.

Intermittent system pressure drops.
Wrench 14 mm
Wrench 17 mm
Parts required Complete valve G1361-60000
Valve G1361-60009
NOTE For this procedure it is especially important to follow the instructions given
below. A failure to align the EMPV valve correctly might result in a failure of
the EMPV or in a defective EMPV.
Changing the valve

1 Using a 1/4 inch wrench disconnect the capillaries:
• going to the system.
• coming from the multi assembly.
2 Disconnect the waste tube. Beware of leaking solvents due to hydrostatic
pressure.
3 Using the 14 mm wrench unscrew the locknut, while using a 7/16 inch wrench
to counter-hold the valve.
4 Unscrew the EMPV valve.
5 Using your hand screw the new valve into the valve body until you feel the first
resistance (Verify the position, where the resistance starts, several times).
6 From the point of first resistance unscrew the valve exactly 1/4 turn back
(counterclockwise).
7 Using a 7/16 inch wrench hold the valve in position and tighten the locknut
using a 14 mm wrench.
8 Rotate the valve so that the waste port points down.
121
Repairing the Pump
9 Using a 1/4 inch wrench reconnect the capillaries:

10 Test proper opening and closing of the valve, by switching the valve on and off
at a low flow rate (Pressure should be below 5 bar with open purge valve at a
flow rate of 10 ml/min with pure water. There should be no visible flow
through the purge valve waste tubing, if the purge valve is closed). Run a leak
test to test the tightness of the valve (see “Leak Test” on page 99).
Figure 28 Electro-Magnetical-Purge-Valve
Valve body
Capillary from filter
EMPV valve
Capillary to system
Screws
122
Repairing the Pump
Exchanging the Complete EMPV

1 Turn off the pump.
2 Remove the pump from the stack and remove the top cover and foam.
3 Using a 1/4 inch wrench disconnect the capillaries:
4 Disconnect the waste tube. Beware of leaking solvents due to hydrostatic
pressure.
5 Disconnect the EMPV connector (P18) on the main board.
6 Using a hex key, unscrew the complete EMPV and remove it.
7 Screw a new one in place.
8 Return to “changing the valve” step 9 in this section and follow the information
step by step.
123
Repairing the Pump
Exchanging the Seal Wash Pump Cassette
Exchanging the Seal Wash Pump Cassette
When required If mechanically defect

Tools required none
Parts required Cassette 5042-6401
1 Disconnect seal wash tubing at the connectors, and place the ends of each
tubing at a level above seal wash bottle (to avoid leaking solvents).
2 By pressing the upper and lower clip remove the seal wash cassette.
3 Replace the defective cassette and slide the new cassette on to the shaft
pressing upper and lower clip. Push forward until the clips snap into place.
The preferred orientation of the cassette is as shown in picture below.
4 Reconnect seal wash tubing.
Figure 29 Seal wash pump

Seal Wash connectors
124
Repairing the Pump
Removing and Disassembling the Pump Head Assembly
Removing and Disassembling the Pump

Head Assembly
WAR NI N G Never start the pump when the pump head is removed. This may
damage the pump drive.
NOTE Seals should always be replaced as pairs (2x front seals + 2x back seals)!
When required: 1 Disconnect the capillaries at the outlet valves

and the tubes at the inlet valves. Beware of
❏ When leaky or blocked leaking solvents. Remove seal wash tubes.
❏ For exchanging the seals
❏ For exchanging the plungers
Outlet Capillaries
Tools required:
❏ Screwdriver Pozidrive#1
❏ 4-mm hexagonal key
❏ 10-mm wrench
❏ Toothpick
Preparations for this procedure:
❏ Switch off preparative pump at power switch
❏ Remove the front cover to have access to the Inlet
pump mechanics tubings
125
Repairing the Pump
2 Using a 4-mm hexagonal key step wise loosen 3 Place the pump head on a flat surface
and remove the four pump head screws and facedown. Pull out the plungers from the
remove the pump head from the pump drive. spring assembly.
Pump head screws Plungers
Spring assembly
4 Loosen the two screws on the spring 5 Remove secondary seals between the
assembly and carefully pull the spring pumphead and the spring assembly, by using
assembly and pumphead apart. the plungers.
Secondary seals
Screws
Spring assembly
Hold here
Pump head
126
Repairing the Pump
6 Using a 10-mm wrench loosen and remove the 7 Carefully lift up the rear pump head.
two special screws.
Special screws
Pump head rear
Pump head front
8 Carefully remove the seals from the pump 9 Remove the O-rings from the rear pump head
head front using the pistons. using a toothpick, if they require
replacement.
O-rings
Seals
Pump head rear

Pump head front
127
Repairing the Pump
Reassembling the Pump Head Assembly
Tools required: 1 Place front pump head facedown on a flat

surface. Insert new seals with the spring
❏ Screwdriver Pozidrive#1 facing the pump body. Press the seals firmly
❏ 4-mm hexagonal key into place. Make sure not to damage seal
surface.
❏ 10-mm wrench
Preparations for this procedure:
Pump seals
❏ Clean all parts before reassembly
Pump head front
128
Repairing the Pump
2 Take rear pump head and place on top of 3 Insert and tighten special screws using a
front pump head. Beware that the O-rings are 10 mm wrench.
in proper place and the seal wash fitting is
facing in same direction as outlet valves.
Special screws
Seal wash fitting
Outlet ball valve
4 Insert new secondary seals with the spring 5 Place Spring assembly on special screws and
facing the pump body. Press the seals firmly tighten the two flathead screws using a
into place. pozidrive#1. The RF-tag must be on the upper
side of the pumphead.
Flathead screws
Seals
Spring assembly
RF-tag
129
Repairing the Pump
6 Reinsert Plungers by pushing until Plunger collars hit springs.
Plungers
130
Repairing the Pump
Exchanging the Optional Interface Board
Exchanging the Optional Interface Board
CA UT IO N The interface board is sensitive to electrostatic discharge. Always use the ESD
kit when handling electronic boards.
When required Board defective

Part required BCD (Interface) board, see “Optional Interface Boards” on page 208
1 Switch off the preparative pump at the main power switch. Unplug the pump
from main power.
2 Disconnect cables from the interface board connectors.
3 Loosen the screws. Slide out the interface board from the preparative pump.
4 Install the new interface board. Secure screws.
5 Reconnect the cables to the board connector.
Figure 30 Exchanging the Interface Board
BCD (Interface) board
131
Repairing the Pump
WAR NI N G The following procedures require opening the main cover of the
preparative pump. Always ensure the preparative pump is
disconnected from the line power when the main cover is removed. The
security lever at the power input socket prevents that the pump cover
is taken off when line power is still connected. To prevent personal
injury, the power cable must be removed from the preparative pump
before opening the pump cover. Do not connect the power cable to the
preparative pump while the covers are removed.
The power supply still uses some power, even if the switch on the front
panel is turned off.
NOTE The electronics of the preparative pump will not allow operation of the
preparative pump when the top cover and the top foam are removed. A safety
light switch on the main board will inhibit the operation of the preparative
pump. Always operate the preparative pump with the top foam and top covers
in place.
CA UT IO N Internal components may be sensitive to electrostatic discharge (ESD).

Always use an ESD kit when handling internal parts.
132
Repairing the Pump
The procedures in this section describe how to exchange internal parts. You
must remove the preparative pump from the stack in order to open the main
cover.
Table 9 Exchanging Internal Parts
Procedure Symptom Potential causes

“Removing the Top Cover and Foam” on To access defective internal Defective top foam cover
page 134 parts, “Top foam cover
missing” error message
“Exchanging the Multi Assembly” on page 136 leaky or blocked multi Defective multi assembly or pressure sensor
assembly, no or wrong
pressure readings from
pressure sensor
“Exchanging the Preparative Pump Main Board (Intermittent) various error Board defective
(PPM Board)” on page 138 messages
“Exchanging the Fan” on page 143 Fan error Fan defective
“Exchanging or Maintaining the Pump Drive” on Bad or no movement of Pump drive defective
page 144 drive, various error
messages
“Exchanging Power Supply” on page 145 No or unstable power Defective power supply
“Exchanging the Leak Sensor” on page 146 Leak sensor error Defective leak sensor
“Exchanging the Seal Wash Pump Drive” on No or bad seal wash motor Defective motor seal wash pump
page 147 movement
“Exchanging Power Switch or Light Pipe” on Defective switch or light pipe
page 148
“Exchanging Status Light Pipe” on page 149 Defective Light pipe
“Assembling the Main Cover” on page 150 Broken or contaminated cover
“Replacing the Top Cover and Foam” on After an internal repair
page 151
133
Removing the Top Cover and Foam
Tools required Screwdriver Pozidriv #1
Preparations for this Switch off preparative pump at the main power switch and unplug the power cable.
procedure Disconnect the solvent inlet tubes from the inlet ball valve.
Beware of leaking solvents due to hydrostatic flow.
Remove leak funnel with the waste tube.
Remove the solvent cabinet from the preparative pump.
1 Remove the front cover by pressing the clip 2 Unplug the power cord and move the lever
fastener on both sides of the cover. towards the power socket.
(1)
Cover clip
Clip fastener (1)
134
Repairing the Pump
Removing the Top Cover and Foam
3 Lift the clips on both sides of the top cover 4 Unscrew the screws on the top plate and
(1). Remove the top cover (2). remove the plate by lifting its back first and
then sliding to the front.
(2)
(2) Screws
(1)
Cover (1)
5 If an optional interface board is installed, 6 Remove the top foam.

remove it from the preparative pump.
135
Repairing the Pump
Exchanging the Multi Assembly
When required Blocked or leaky multi assembly, defective pressure sensor

Preparations for this Switch off preparative pump at the main power switch and unplug the power cable.
procedure Remove top covers and foam (see “Removing the Top Cover and Foam” on page 134)
Parts required Multi assembly, PN G1361-60005
1 Remove top covers and foam (see “Removing 2 Unscrew the z-plane and lift it out of the
the Top Cover and Foam” on page 134). housing of the preparative pump.
136
Repairing the Pump
3 Remove all capillaries connected to the multi 4 Loosen the two holding screws of the multi
assembly. assembly.
(2) (3)
Screws
(1)
5 Disconnect the pressure sensor cable (P29) 6 Perform the opposite steps in reverse order to
from the PPM board and lift out the multi install a new multi assembly into the pump.
assembly from the pump.
137
Repairing the Pump
Exchanging the Preparative Pump Main Board (PPM Board)
Exchanging the Preparative Pump Main

Board (PPM Board)
When required Board defective

Wrench 5 mm
Parts required PPM board, PN G1361-66500 or exchange PN G1361-69500
1 Turn off the pump, disconnect all cables and remove the pump from the stack.
2 Remove the top covers and foam, see “Removing the Top Cover and Foam” on
page 134.
3 Disconnect all connectors from the main board.
Connector Description Connector Description
S1 Configuration switch P18 Motor U+

P13 Power supply main logic P19 Motor U-
P4 CAN connector P30 Leak sensor
P5 CAN connector P29 Multi Assembly
P6 RS232 P27 Fan
P7 Remote P17 Motor index
P28 Motor encoder P8 Power switch board
P10 Power supply 24V P2 CAN-DC-out
P25 MIO P3 CAN-DC-out
P20 TAG P22 EMPV
P23 Seal wash pump DS6-DS8 Status LED
NOTE When removing connectors, counterhold on connector P25 with one hand.
138
Repairing the Pump
Figure 31 Board Layout of the PPM Board
P4 P5 P6 P7
S1
P2 P3
P10
P8
P13
P17
P18 P19
P22
P23 P20
P25
P28 P29 P30 P27
4 Remove the connector screws from the remote connector and the additional
holding screw between the CAN interface ports and the CAN-DC OUT.
5 Remove the board. Place it on an ESD kit.
WAR NI N G The RFI spring plate sitting on the board connectors is very sharp! Be
careful, not to cut yourself, when removing it from the old board and
sliding it onto the new board.
6 In most cases the RFI spring plate (Radio Frequency Interference spring plate,
prevents radio emissions from the instrument to environment) remains on the
interface connectors of the board. Carefully remove the spring plate from the
old board and slide onto the new board before installing the new board in the
pump (the RFI spring plate is NOT part of an exchange board).
7 On the new board check the switch setting of address switch S1, see “Setting
the 8-bit Configuration Switch” on page 215.
139
Repairing the Pump
NOTE An incorrect switch setting (e.g., TEST/BOOT) may cause the pump to turn
into a basic mode (yellow or red flashing status light). In such a case turn off
the pump, re-set the address switches, and turn on the pump again.
8 Install the new board and reconnect the connectors. Make sure that the board
is fitted correctly in the board recess holes at the rear panel.
9 Replace the connector and holding screws.
Figure 32 Rear of preparative pump
Screws
10 Replace foam and top covers, see “Replacing the Top Cover and Foam” on
page 151.
11 Reinstall the pump in the stack. Reconnect all cables. Turn on the preparative
pump. Continue with the section “Entering the Serial Number” on page 140.
Entering the Serial Number

Entering the Serial Number using the Control Module
1 Connect the control module to the pump. Turn on the pump.
2 In the control module, press Views (F5) and select the system screen, then
press Records (F4). Using the up/down arrows, make sure that the
preparative pump is highlighted.
140
Repairing the Pump
3 Press FW Update (F5). Now, press the m key. This will display a box which
says Update Enter Serial#.
4 Press Enter. This will display the box labeled Serial#.
5 Letters and numbers are created using the up and down arrows. Into the box
labeled Serial#, enter the 10-character serial number for the preparative
pump. When the 10-character serial number is entered, press Enter to
highlight the complete serial number. Then, press Done (F6).
NOTE For firmware revisions below A02.00 it is very important never to press Done
if the Serial# box is blank. In this case, the module can no longer be recognized
by either the control module or the ChemStation. The main board must then
be replaced.
6 Turn the micro pump off, then on again. The Records screen should display the
correct serial number.
7 If a ChemStation is also connected, re-boot the ChemStation now as well.
Entering the Serial Number using the ChemStation

Module serial numbers are entered by typing specific commands into the
command line at the bottom of the main user interface screen.
1 To enter a module serial number, type the following command into the
command line:
print sendmodule$(lpmp, "ser YYYYYYYYYY")
Where: YYYYYYYYYY is the 10-character serial number of the preparative
pump
NOTE The first two characters are letters, which should be capitalized.
The reply line will respond with RA 0000 SER followed by the module serial
number you just entered.
2 Turn off the preparative pump, then on again. Then, re-boot the ChemStation.
If the serial number you have just entered is different than the original module
serial number, you will be given the opportunity to edit the configure 1100
access screen during the re-boot of the ChemStation.
141
Repairing the Pump
3 After boot-up, the serial number you have just entered can be seen under the
Instrument menu of the main user interface screen. The serial number of the
preparative pump can also be seen by typing the following command into the
command line:
print sendmodule$(lpmp, "ser?") The reply line will give the serial number of
Replacing the Preparative Pump’s Firmware

The installation of new firmware is required
• if a new version solves problems of the currently installed version.
• if the version of firmware on the new main board (CSM) after an exchange
of the board is older than the one previously installed.
To upgrade the preparative pump’s firmware follow the procedures and
instructions given on the internet at:
http://www.agilent.com/chem
To download and install always the newest available version of firmware on

your system or call your local service provider for assistance.
142
Repairing the Pump
Exchanging the Fan
Exchanging the Fan
When required: Defective fan

Tools required: Screwdriver Pozidriv #1
Parts required: Fan, PN 3160-1017
1 Remove the top covers (see “Removing the Top Cover and Foam” on page
134).
2 Disconnect the fan cable (P95) at the main board and move the wires from the
multi assembly and the EMPV out of the way. Slide the fan upwards and move
it out of the unit.
Figure 33 Replacing the fan
3 Place the new fan into the recess. Make sure that the air flow is directed as
indicated (arrow on the fan points in the same direction as on the foam).
Connect the cable to the main board (P95).
4 Replace the top foam section, optional interface board, front cover, and top
cover (see “Replacing the Top Cover and Foam” on page 151)
143
Repairing the Pump
Exchanging or Maintaining the Pump Drive
Exchanging or Maintaining the Pump

Drive
When required: Pump drive defective

Parts required: Pump drive assembly, PN G1361-60001
or
Pump drive exchange assembly, PN G1361-69001
WAR NI N G This is not a user-performable task. This part does not contain any
user serviceable parts. For safety reasons it is absolutely forbidden
that this task is performed by a customer! To avoid personal injury call
an Agilent Technologies service engineer or any service engineer
authorized by Agilent Technologies to perform this task.
144
Repairing the Pump
Exchanging Power Supply
Exchanging Power Supply
When required: Defective power supply

Parts required: Power supply, PN 0950-3907
145
Repairing the Pump
Exchanging the Leak Sensor
Exchanging the Leak Sensor
When required: Defective leak sensor

Parts required: Leak Sensor, PN 5061-3356
146
Repairing the Pump
Exchanging the Seal Wash Pump Drive
Exchanging the Seal Wash Pump Drive
When required: Defective seal wash pump motor

Parts required: Stepper motor - seal wash pump, PN 5065-4409
147
Repairing the Pump
Exchanging Power Switch or Light Pipe
Exchanging Power Switch or Light Pipe
When required: Defective switch or light pipe

Parts required: Power switch button, PN 5041-8381 or light pipe - power switch, PN 5041-8382
148
Repairing the Pump
Exchanging Status Light Pipe
Exchanging Status Light Pipe
When required: Defective status light pipe

Tools required: Screwdriver Pozidriv #1
Part required: Status light pipe 5041-8384
Preparation for this procedure:

❏ Remove the front cover and top cover, see “Removing the Top Cover and Foam” on page 134.
1 The status light pipe is clipped into the top 2 Replace the top cover, see “Replacing the Top
cover. Cover and Foam” on page 151.
3 Replace the preparative pump into the stack
and reconnect the cables and capillaries.
4 Turn on the preparative pump.
149
Repairing the Pump
Assembling the Main Cover
Assembling the Main Cover
When required Broken or contaminated cover

Tools required None
Parts required Cover kit G1361-68703 (includes base, top, left and right)
NOTE The cover kit contains all parts, but it is not assembled.
WAR NI N G In case you insert the left or right left / right side in the wrong
orientation or into the wrong side of the top cover, you may not be able
to remove the side from the top part.
1 Place the top part on the bench and insert the 2 Replace the cover.
left and right side into the top part.
Front
3 Replace the preparative pump into the stack and reconnect the cables and capillaries.
4 Turn on the preparative pump.
150
Repairing the Pump
Replacing the Top Cover and Foam

Preparations for this Make sure that after your repair all assemblies, cables, capillaries and connectors are
procedure located in its correct place.
1 Replace the top foam section. 2 Make sure that the foam is installed correctly.
151
Repairing the Pump
3 Location of foam in the light switch. 4 Replace the optional interface board or the
board cover plate.
Light switch
5 Replace the metal cover (slide the metal tabs 6 Replace the top cover.
into place (1) underneath the Z-Panel in the
front, then lower the back of the metal plate
(2)) and fix the two holding screws.
(2)
(1)
Holding
screws
152
Repairing the Pump
7 Ensure clips are seated correctly, move lever 8 Replace the leak funnel with the waste tube.
back. Locate the lower end of the waste tube in the
holder of the leak pan.
Waste funnel
Lever
Waste tube holder

Clip Leak pan
9 Place the preparative pump back to its Replace the front covers and reconnect the
position in the stack, replace solvent cabinet power cable to the preparative pump.
and reconnect all tubes and capillaries. Switch on the preparative pump.
153
Repairing the Pump
154
5
5 Parts and Materials
Detailed illustrations and lists for identification

of parts and materials
Parts and Materials
Pump Housing and Main Assemblies
Table 10 Repair Parts — Pump Housing and Main Assemblies (Front View)
Item Description Part Number
1 Pump drive assembly G1361-60001

Exchange assembly — pump drive G1361-69001
2 Pump head, see page 162 G1361-60004
3 Outlet valve, see page 162
4 Inlet valve, see page 162
5 Cap, seal wash, see page 162
6 Main Board PPM G1361-66500
Exchange assembly — Main Board PPM G1361-69500
7 Power supply, underneath the PPM board 0950-3907
8 Fan assembly 3160-1016
9 Leak pan - pump 5041-8390
10 Leak sensor 5061-3356
11 Multi assembly with filters and pressure sensor (at the rear) G1361-60005
Filter cup 2 µm 3150-0942
SST filter assembly 5022-2192
12 Electromagnetic purge valve (EMPV), see page 164 G1361-60000
13 Cassette — seal wash pump 5042-6401
Stepper motor — seal wash pump 5065-4409
156
Parts and Materials
Figure 34 Overview of Main Assemblies (Front View)
1 6
5
8
3
2
4
12
11
10
13
157
Parts and Materials
Table 11 Repair Parts — Pump Housing and Main Assemblies (Rear View)
1 Standoff — remote connector 1251-7788

2 Power supply (behind rear panel) 0950-3907
3 Screw 0515-0910
Lock washer 2190-0409
4 Board cover 5001-3772
Figure 35 Overview of Main Assemblies (Rear View)
3
4
158
Parts and Materials
Solvent Cabinet and Bottle-Head Assembly
Solvent Cabinet and Bottle-Head Assembly
Table 12 Solvent Cabinet and Bottle-Head Assembly Parts
1 Solvent cabinet, including all plastic parts 5062-8581

2 Name plate, Agilent 1100 5042-1381
3 Front panel, solvent cabinet 5062-8580
4 Leak pan, solvent cabinet 5042-1307
5 Bottle amber, 2 liter 9301-6341
Bottle clear, 2 liter, with second inlet 5065-4421
6 Bottle-head assembly for Prep pump G1361-60002
includes items 6, 7, 8, 9, and 10
7 Cap, bottle
8 Y-fitting and tube pump
9 Frit adapter G1361-23204
10 Solvent inlet filter 3150-0944
Figure 36 Solvent Cabinet Parts
8
1
7
2
3 5
9, 10
4
159
Parts and Materials
Hydraulic Path
Hydraulic Path
Table 13 Hydraulic Path
1 Tubing from solvent bottle, see “Solvent Cabinet and Bottle-Head G1361-60002
Assembly” on page 159)
2, 3 Tube clip, Leak funnel 5041-8387, 5041-8388
4 Leak drainage, same as item 7 (re-order 5 m) 5062-2463
5 Prep Capillary 3 (basis kit), EMPV out to next module, G1361-67302
SST 1/16” o.d., 0.6 mm i.d., 400 mm long
5a Prep Capillary 4, multi assembly to EMPV, G1361-67303
6 Tubing PTFE 0.057” i.d., EMPV to waste (re-order 5 m) 5062-2461
7 Corrugated waste tube, 120 cm (re-order 5 m) 5062-2463
8 Prep Capillary 1, Outlet valve 1 to multi assembly top, G1361-67300
9 Prep Capillary 2, Outlet valve 2 to multi assembly bottom, G1361-67301
10 Outlet ball valve, see “Pump-Head Assembly” on page 162
11 Inlet ball valve, see “Pump-Head Assembly” on page 184
12 Tubing, Seal wash to waste 0890-1764
13 Tubing, Seal wash solvent bottle to seal wash pump 0890-1764
14 Tubing, Seal wash pump to pump head 0890-1764
Tube adapter (for item 13 and 14) 5042-6422
15 Prep Capillary 5 (gradient kit), Union to EMPV2 G1361-67304
16 Prep Capillary 6 (gradient kit), Union to mixer G1361-67305
17 Prep Capillary 7 (gradient kit), EMPV1 to union G1361-67306
160
Parts and Materials
Hydraulic Path
Table 13 Hydraulic Path
18 Union (gradient kit) 0100-1016

Fitting FRONT, Fitting BACK, Fitting 1/16”, re-order 10/pk 5062-2418
Figure 37 Hydraulic Path

1
2
8
3
9
12 4
10
5a
11
5
13 6
14
7
15
Gradient interconnection
Pump 1
EMPV 1
17
Pump 2
EMPV 2 Union 18
15
16
Mixer Column
5
161
Parts and Materials
Pump-Head Assembly
Pump-Head Assembly
Table 14 Pump-Head Assembly
Complete assembly G1361-60004

1 Sapphire plunger G1361-22402
2 Pump head front G1361-25200
3 Pump head rear G1361-25201
4 O-ring 0905-1516
6 Plunger housing see note below
7 Cap seal wash fittings G1361-67307
8 Seal prep flange 5022-2188
9 Valve assembly for inlet and outlet G1361-60012
10 Adapter for outlet valve G1361-23202
11 Adapter for inlet valve G1361-23201
12 Screw M5, 55 mm lg 0515-4249
13 Special screw M6 G1361-22401
14 Screw for plunger housing see note below
NOTE Item 6 (plunger housing) and 14 (screw) are not available for replacement at
introduction. A complete assembly will be made available at a later stage.
162
Parts and Materials
Pump-Head Assembly
Figure 38 Pump-Head Assembly
14
7
1
13
6
10 8
9
3
2 8
12
11
163
Parts and Materials
Electro Mechanical Proportional Valve (EMPV)
Electro Mechanical Proportional Valve

(EMPV)
Table 15 EMPV Assembly
EMPV — complete assembly G1361-60000

1 EMPV solenoid assembly 5064-8283
2 Proportional Valve body G1361-60009
3 Prep Capillary 4, multi assembly to EMPV, G1361-67303
4 Prep Capillary 3 (basis kit), EMPV out to next module, G1361-67302
5 Screw M4, 40 mm long 0515-0850
Figure 39 EMPV Assembly
3
All fittings 1/16”
2
Fitting FRONT, Fitting
5 BACK, Fitting 1/16”,
re-order 10/pk
5062-2418
164
Parts and Materials
Multi Assembly Parts
Multi Assembly Parts
Table 16 Multi Assembly Parts
Multi assembly with filters and pressure sensor (at the rear) G1361-60005
1 Filter screw assembly, includes item 2 G1361-60006
2 Holder filter G1361-22300
3 Filter cup 3150-0942
4 Filter screw G1361-44601
5, 6 SST Filter assembly 2 µm 5022-2192
Figure 40 Multi Assembly Parts
Note:
5 Item 6 is part of item 5
165
Parts and Materials
Cover Parts
Cover Parts
Table 17 Cover Parts
1 Plastic cover kit (includes top, base and both sides) G1361-68703
2 Front plate G1361-68704
3 Logo plate, Agilent 1100 5042-1381
Figure 41 Cover Parts
166
Parts and Materials
Sheet Metal Kit
Sheet Metal Kit
Table 18 Sheet Metal Kit
1 Sheet metal kit, includes top, base and Z-panel G1361-68701
2* Screw, for cover and Z-Panel 5022-2112
3* Board cover 5001-3772
* Included in item 1
Figure 42 Sheet Metal Kit

2
167
Parts and Materials
Foam Parts
Foam Parts
Table 19 Foam Parts
1 Foam kit, including transport, upper and lower foam G1361-68702

2 Bushing, for pump drive (yellow, 5x) not shown 1520-0404
3 Bushing, for pump drive (blue, 5x) not shown 5041-8381
4 Board guide 5041-8395
Figure 43 Foam Parts
upper foam
lower foam
transport foam
168
Parts and Materials
Preparative Pump Basis Kit
Preparative Pump Basis Kit
This kit is part of the G1361A Preparative Pump.

Tube seal wash, 2m 0890-1764 1
Waste tube, reorder No. 5042-2461 1
Capillary 3, pump outlet to system G1361-67302 1
Bottle AMBER 2,0 l 9301-6341 1
Wrench open end 14-17 mm 8710-2435 1
Wrench open end 1/4-5/16" 8710-0510 2
Wrench open end 7/16-3/8” 8710-0972 1
Hex-key set 8710-0641 1
Pair of tweezers 5022-2195 1
Spare SST filter assembly 2 µm 5022-2192 1
CAN cable, 1 m 5181-1519 1
Union preparative 5022-2133 1
Sanding paper no PN 2
ESD wrist-strap 9300-1408 1
169
Parts and Materials
Preparative Pump Gradient Kit
Preparative Pump Gradient Kit
This kit is part of the G1391A Gradient Extension
Table 21 Gradient Kit Contents G1361-68707 (shipped with every additional pump in a
gradient system)

Tube seal wash 0890-1764 1
Waste tube 5042-2461 1
Bottle, clear 2,0 l 9301-6342 1
Capillary 5, pump A to T-union G1361-67304 1
Capillary 6, T-union to inlet of mixer G1361-67305 1
Female-T-union 0100-1016 1
Mixer 79835-87330 1
Capillary 7, pump B to T-union G1361-67306 1
Spare SST filter assembly 2 µm 5022-2192 1
CAN cable, 0.5 m 5181-1516 1
170
Parts and Materials
Control Module (G1323B)
Control Module (G1323B)
Table 22 Control Module Parts
Control Module, replacement part including cable G1323-67001

Plastic Housing Kit, includes front, back and a clamp 5062-8583
CAN cable Agilent 1100 module to control module G1323-81600
Figure 44 Control Module
171
Parts and Materials
Power and Status Light Pipes
Power and Status Light Pipes
Table 23 Power and Status Light Pipes
1 Power switch coupler 5041-8383

2 Light pipe — power switch 5041-8382
3 Power switch button 5041-8381
4 Light pipe — status lamp 5041-8384
Figure 45 Power and Status Light Pipes
3
4
172
Parts and Materials
Leak Parts
Leak Parts
Table 24 Leak Parts
1 Leak sensor 5061-3356

2 Tube clip 5042-6405
3 Leak plane, pump 5041-8390
4 Leak funnel 5042-6404
5 Holder, leak funnel 5041-8389
6 Corrugated waste tube (reorder pack, 5 m) 5062-2463
Figure 46 Leak Parts
2 1
5
4
173
Cable Overview
regulations.
Table 25 Cables Overview
Type Description Part Number
Analog 3390/2/3 integrators 01040-60101

cables
3394/6 integrators 35900-60750
Agilent 35900A A/D converter 35900-60750
General purpose (spade lugs) 01046-60105
Remote 3390 integrator 01046-60203
cables
3392/3 integrators 01046-60206
3394 integrator 01046-60210
3396A (Series I) integrator 03394-60600
3396 Series II / 3395A integrator, see page 180
3396 Series III / 3395B integrator 03396-61010
HP 1050 modules / HP 1046A FLD 5061-3378
HP 1046A FLD 5061-3378
Agilent 35900A A/D converter 5061-3378
HP 1040 diode-array detector 01046-60202
HP 1090 liquid chromatographs 01046-60202
Signal distribution module 01046-60202
BCD cables 3392/3 integrators 18594-60510
174
Parts and Materials
Cable Overview
Table 25 Cables Overview, continued
Type Description Part Number
3396 integrator 03396-60560

General purpose (spade Lugs) 18594-60520
Auxiliary Agilent 1100 Series vacuum degasser G1322-61600
CAN Agilent 1100 module to module,0.5m lg 5181-1516
cables Agilent 1100 module to module, 1m lg 5181-1519
Agilent 1100 module to control module G1323-81600
External Agilent 1100 Series interface board to general G1103-61611
contacts purpose
GPIB cable Agilent 1100 module to ChemStation, 1 m 10833A
Agilent 1100 module to ChemStation, 2 m 10833B
RS-232 Agilent 1100 module to a computer 34398A
cable This kit contains a 9-pin female to 9-pin female Null
Modem (printer) cable and one adapter.
LAN Twisted pair cross over LAN cable, 10 feet long 5183-4649
cable (for point to point connection)
Category 5 UTP cable, 8 m long G1530-61480
(for hub connections)
175
Parts and Materials
Analog Cables
Analog Cables
One end of these cables provides a BNC connector to be connected to

Agilent 1100 Series modules. The other end depends on the instrument to
which connection is being made.
Agilent 1100 to 3390/2/3 Integrators
Connector Pin Pin

01040-60101 3390/2/3 Agilent 1100 Signal Name
1 Shield Ground
2 Not connected
3 Center Signal +
4 Connected to pin 6
5 Shield Analog -
6 Connected to pin 4
7 Key
8 Not connected
Agilent 1100 to 3394/6 Integrators
Connector Pin Pin

35900-60750 3394/6 Agilent 1100 Signal Name
1 Not connected
2 Shield Analog -
3 Center Analog +
176
Parts and Materials
Analog Cables
Agilent 1100 to BNC Connector
Connector Pin Pin

8120-1840 BNC Agilent 1100 Signal Name
Shield Shield Analog -

Center Center Analog +
Agilent 1100 to General Purpose
Connector Pin Pin

01046-60105 3394/6 Agilent 1100 Signal Name
1 Not connected
2 Black Analog -
3 Red Analog +
177
Parts and Materials
Remote Cables
Remote Cables
One end of these cables provides a Agilent Technologies APG (Analytical

Products Group) remote connector to be connected to Agilent 1100 Series
modules. The other end depends on the instrument to be connected to.
Agilent 1100 to 3390 Integrators
Connector Pin Pin Active

01046-60203 3390 Agilent 1100 Signal Name (TTL)
2 1 - White Digital ground

NC 2 - Brown Prepare run Low
7 3 - Gray Start Low
NC 4 - Blue Shut down Low
NC 5 - Pink Not connected
NC 6 - Yellow Power on High
NC 7 - Red Ready High
NC 8 - Green Stop Low
NC 9 - Black Start request Low
178
Parts and Materials
Remote Cables

01046-60206 3392/3 Agilent 1100 Signal Name (TTL)

9 7 - Red Ready High
1 8 - Green Stop Low
4 - Key


5,14 7 - Red Ready High
1 9 - Black Start request Low
13, 15 Not connected
NOTE START and STOP are connected via diodes to pin 3 of the 3394 connector.
179
Parts and Materials
Remote Cables
Agilent 1100 to 3396A Integrators


5,14 7 - Red Ready High
Agilent 1100 to 3396 Series II / 3395A Integrators

Use the cable 03394-60600 and cut pin #5 on the integrator side. Otherwise
the integrator prints START; not ready.
180
Parts and Materials
Remote Cables
Agilent 1100 to 3396 Series III / 3395B Integrators

03396-61010 33XX Agilent 1100 Signal Name (TTL)

Agilent 1100 to HP 1050, HP 1046A or Agilent 35900 A/D Converters

5061-3378 HP 1050/.... Agilent 1100 Signal Name (TTL)
1 - White 1 - White Digital ground

2 - Brown 2 - Brown Prepare run Low
3 - Gray 3 - Gray Start Low
4 - Blue 4 - Blue Shut down Low
5 - Pink 5 - Pink Not connected
6 - Yellow 6 - Yellow Power on High
7 - Red 7 - Red Ready High
8 - Green 8 - Green Stop Low
9 - Black 9 - Black Start request Low
181
Parts and Materials
Remote Cables
Agilent 1100 to HP 1090 LC, HP 1040 DAD or Signal Distribution

Module

01046-60202 HP 1090 Agilent 1100 Signal Name (TTL)
7 4 - Blue Shut down Low
8 5 - Pink Not connected
5 - Key

01046-60201 Universal Agilent 1100 Signal Name (TTL)
1 - White Digital ground

2 - Brown Prepare run Low
3 - Gray Start Low
4 - Blue Shut down Low
5 - Pink Not connected
6 - Yellow Power on High
7 - Red Ready High
8 - Green Stop Low
9 - Black Start request Low
182
Parts and Materials
BCD Cables
8 7 6 5 4 3 2 1
BCD Cables 15 14 13 12 11 10 9
One end of these cables provides a 15-pin BCD connector to be connected to

the Agilent 1100 Series modules. The other end depends on the instrument to
be connected to.
Connector Pin Pin

18584-60510 3392/3 Agilent 1100 Signal Name BCD Digit
10 1 BCD 5 20
11 2 BCD 7 80
3 3 BCD 6 40
9 4 BCD 4 10
7 5 BCD 0\ 1
5 6 BCD 3 8
12 7 BCD 2 4
4 8 BCD 1 2
6 - Key
1 9 Digital ground
2 15 +5V Low
183
Parts and Materials
BCD Cables
Connector Pin Pin

03396-60560 3392/3 Agilent 1100 Signal Name BCD Digit
1 1 BCD 5 20
2 2 BCD 7 80
3 3 BCD 6 40
4 4 BCD 4 10
5 5 BCD 0\ 1
6 6 BCD 3 8
7 7 BCD 2 4
8 8 BCD 1 2
9 9 Digital ground
NC 15 +5V Low
Connector Pin
18594-60520 Wire Color Agilent 1100 Signal Name BCD Digit
Green 1 BCD 5 20
Violet 2 BCD 7 80
Blue 3 BCD 6 40
Yellow 4 BCD 4 10
Black 5 BCD 0\ 1
Orange 6 BCD 3 8
Red 7 BCD 2 4
Brown 8 BCD 1 2
Gray 9 Digital ground
White 15 +5 Vt Low
184
Parts and Materials
Auxiliary Cable
Auxiliary Cable
One end of this cable provides a modular plug to be connected to the

Agilent 1100 Series vacuum degasser. The other end is for general purpose.
Agilent 1100 Series Degasser to general purposes
Connector Pin
G1322-81600 Color Agilent 1100 Signal Name
White 1 Ground
Brown 2 Pressure signal
Green 3
Yellow 4
Grey 5 DC + 5 V IN
Pink 6 Vent
CAN Cable
Both ends of this cable provide a modular plug to be connected to

Agilent 1100 Series module’s CAN-bus connectors.
Agilent 1100 module to module, 0.5m 5181-1516

lg
Agilent 1100 module to module, 1m lg 5181-1519
Agilent 1100 module to control G1323-81600
module
185
Parts and Materials
External Contact Cable
5 1
External Contact Cable 10
15 11
6
One end of this cable provides a 15-pin plug to be connected to Agilent 1100
Series module’s interface board. The other end is for general purpose.
Agilent 1100 Series Interface Board to general purposes
Connector Pin
G1103-61611 Color Agilent 1100 Signal Name
White 1 EXT 1
Brown 2 EXT 1
Green 3 EXT 2
Yellow 4 EXT 2
Grey 5 EXT 3
Pink 6 EXT 3
Blue 7 EXT 4
Red 8 EXT 4
Black 9 Not connected
Violet 10 Not connected
Grey/pink 11 Not connected
Red/blue 12 Not connected
White/green 13 Not connected
Brown/green 14 Not connected
White/yellow 156 Not connected
186
Parts and Materials
RS-232 Cable Kit
RS-232 Cable Kit
This kit contains a 9-pin female to 9-pin female Null Modem (printer) cable
and one adapter. Use the cable and adapter to connect Agilent Technologies
instruments with 9-pin male RS-232 connectors to most PCs or printers.
Agilent 1100 module to PC
RS-232 Cable Kit 34398A
Instrument PC
DCD 1 1 DCD
RX 2 2 RX
TX 3 3 TX
DTR 4 4 DTR
GND 5 5 GND
DSR 6 6 DSR
RTS 7 7 RTS
CTS 8 8 CTS
RI 9 9 RI
DB9 DB9 DB9 DB9

Male Female Female Male
187
Parts and Materials
LAN Cables
LAN Cables
Recommended Cables
For point to point connection (not using a network hub) use a twisted pair
cross over LAN cable (P/N 5183-4649, 10 feet long).
For standard network connections using a hub use category 5 UTP cables,
(P/N G1530-61480, 8 m long).
188
6
6 Introduction to the
Preparative Pump
An introduction to the pump, instrument

overview, theory of operation, external
communication and internal connectors
Introduction to the Preparative Pump
The preparative pump is a cam driven pump, with fixed stroke and two
parallel pistons, each with (identical ball and seat) inlet and outlet valve.
Each of the two pump pistons is capable of delivering a maximum of
50 ml/min flow at up to 400 bar pressure.
The two cams have overlapping delivery cycles. Compression losses are
calculated and smooth delivery is ensured through variation of the motor
speed over the pump cycle.
The pistons are guided through two seals each. The gap between those seals
is connected to capillary fittings, this way seal wash is not only an option, but
integral part of every pump. A peristaltic pump, to automate seal wash, is
also part of the system.
190
Figure 47 Overview of the Preparative Pump
Pump drive Power supply
PPM board
Fittings seal wash Fan

Outlet valves
Pump head
Inlet valves
Electromagnetic
Leak plane purge valve
Leak sensor
Seal wash pump
Multi
Solvent
Assy
selection
with filters
valveand
pressure sensor (at the rear)
191
Hydraulic Path Overview

The pump flow leaving the pump head passes through the multi assy, which
combines the flow of the two pistons, filters the solvent and contains a
pressure sensor to monitor system pressure. No hydraulic damper is included
in the system. This makes very fast gradients possible. From the multi assy
the flow is routed to the purge valve and from there either directly into waste
or on to the next module (normally injection valve or sampling device).
Gradients are formed with two combined pumps, as high pressure gradients.
The combined flow, is flowing through a passive mixer, to smooth
composition. With its high pressure mixing principle gradient systems and
the usually high flow rates, no degassing of solvents is necessary.
To avoid outgassing in the detector cell, a back pressure regulating device, is
recommended, for applications, that demand it.
192
Figure 48 Schematics of the preparative pump
Inlet Inlet From Pump B, only

valve 1 valve 2 in binary gradient system
From
solvent Outlet Outlet Female T, only
bottle valve 1 valve 2 in binary
gradient system
Mixer, only in
Seals (2x2)
binary gradient
EMPV system
Piston 1
Multi-Assembly
with 2 filters and
Piston 2 pressure sensor
To Waste
Table 26 Preparative Pump Details
Materials in contact with mobile phase

Bottle head Assy glass frits, PTFE compounds, PEEK
Pump head SST, sapphire
Inlet/Outlet Valves SST, sapphire, ruby, PEEK
EMPV SST, ruby, sapphire, PEEK
Filter Cup SST
Filter Plate SST, PEEK
Pressure Sensor SST, PEEK
Capillaries SST
For pump specifications, see “Specifications” on page 245.
How Does Compressibility Compensation Work?

The compressibility of the solvents in use will affect retention-time stability
when the back pressure in the system changes (for example, aging of
column). In order to minimize this effect, the pump provides a
193
compressibility compensation feature which optimizes the flow stability

according to the solvent type. The compressibility compensation is set to a
default value at the factory. The compensation value can be changed through
the user interface.
Without a compressibility compensation the following will happen during a
stroke of the first piston. The pressure in the piston chamber increases and
the volume in the chamber will be compressed depending on backpressure
and solvent type. The volume displaced into the system will be reduced by
the compressed volume.
When a compressibility compensation value for the pump head is set, the
pump processor calculates a compensation volume that depends on the
system pressure and the selected compressibility value. The pump has a fixed
stroke. To compensate for compressibility losses, the speed of the piston
movement has to be varied during the different parts of each stroke.
194
Electrical Connections
• The CAN bus is a serial bus with high-speed data transfer. The two
connectors for the CAN bus are used for internal Agilent 1100 Series
module data transfer and synchronization.
• The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
common shut down, prepare, and so on.
• The RS-232 connector may be used to control the preparative pump from
a computer through an RS-232 connection, using appropriate software.
This connector needs to be activated by the 8-bit configuration switch
module in the upper right corner of the rear of the pump. The software
needs the appropriate drivers to support this communication. See your
software documentation for further information.
• The power input socket accepts a line voltage of 100 – 240 Volts AC ± 10 %
with a line frequency of 50 - 60 Hz. max. power consumption is 250 VA
(Volt-Amps). There is no voltage selector on your preparative pump
because the power supply has wide-ranging capability. There are no
externally accessible fuses. The security lever at the power input socket
prevents that the preparative pump cover is taken off when line power is
still connected.
• The interface (BCD) board slot is used for external contacts, BCD output
and for LAN communication.
• The CAN-DC-out provides 24 Volts DC power for external CAN devices
like a switch valve. Max. permanent power consumption is
100 mA/channel or 2 A for sec peak consumption.
regulations.
The power supply still uses some power, even if the power switch on
the front panel is turned off.
195
Figure 49 Preparative Pump Electrical Connections

CAN cable to previous module
Interface (BCD) board slot
8-bit configu-
ration switch
Remote
CAN-DC-out
RS232C
CAN cable to next module
CAN-bus
196
Instrument Layout
Instrument Layout
The industrial design of the module incorporates several innovative features.

It uses Agilent’s E-PAC concept for the packaging of electronics and
mechanical assemblies. This concept is based upon the use of expanded
polypropylene (EPP) layers foam plastic spacers in which the mechanical
and electronic boards components of the module are placed. This pack is
then housed in a metal inner cabinet which is enclosed by a plastic external
cabinet. The advantages of this packaging technology are:
• virtual elimination of fixing screws, bolts or ties, reducing the number of
components and increasing the speed of assembly/disassembly,
• the plastic layers have air channels molded into them so that cooling air
can be guided exactly to the required locations,
• the plastic layers help cushion the electronic and mechanical parts from
physical shock, and
• the metal inner cabinet shields the internal electronics from
electromagnetic interference and also helps to reduce or eliminate radio
frequency emissions from the instrument itself.
197
Early Maintenance Feedback (EMF)
Maintenance requires the exchange of components in the flow path which

are subject to mechanical wear or stress. Ideally, the frequency at which
components are exchanged should be based on the intensity of usage of the
instrument and the analytical conditions, and not on a predefined time
interval. The early maintenance feedback (EMF) feature monitors the usage
of specific components in the instrument, and provides feedback when the
user-settable limits have been exceeded. The visual feedback in the user
interface provides an indication that maintenance procedures should be
scheduled.
EMF Counters
The preparative pump provides a series of EMF counters for the pump head.
Each counter increments with pump use, and can be assigned a maximum
limit which provides visual feedback in the user interface when the limit is
exceeded. Each counter can be reset to zero after maintenance has been
done. The preparative pump provides the following EMF counters:
• liquimeter pump head,
• seal wear pump head.
Liquimeter
The liquimeter displays the total volume of solvent pumped by the pump
head since the last reset of the counters. The liquimeter can be assigned an
EMF (maximum) limit. When the limit is exceeded, the EMF flag in the user
interface is displayed.
Seal Wear Counter

The seal wear counter displays a value derived from pressure and flow (both
contribute to seal wear). The value increments with pump usage until the
counter is reset after seal maintenance. The seal wear counter can be
assigned an EMF (maximum) limit. When the limit is exceeded, the EMF flag
in the user interface is displayed.
198
Using the EMF Counters

The user-settable EMF limits for the EMF counters enable the early
maintenance feedback to be adapted to specific user requirements. The wear
of pump components is dependent on the analytical conditions, therefore,
the definition of the maximum limits need to be determined based on the
specific operating conditions of the instrument.
Setting the EMF Limits

The setting of the EMF limits must be optimized over one or two
maintenance cycles. Initially, no EMF limit should be set. When performance
indicates maintenance is necessary, take note of the values displayed by
liquimeters and seal wear counters. Enter these values (or values slightly less
than the displayed values) as EMF limits, and then reset the EMF counters to
zero. The next time the EMF counters exceed the new EMF limits, the EMF
flag will be displayed, providing a reminder that maintenance needs to be
scheduled.
199
The Electronics
The Electronics
The electronics are comprised of four main components:

• The preparative pump main board (PPM), see page 201.
• Power supply, see page 219.
Optional:
• Interface board (BCD/external contacts), see page 208.
• Interface board (LAN), see page 209.
200
The Electronics
Preparative Pump Main Board (PPM)

The board controls all information and activities of all assemblies within the
preparative pump. The operator enters parameters, changes modes and
controls the preparative pump through interfaces (CAN or RS-232C),
connected to the user-interfaces. Figure 50 and Figure 51 show block
diagrams of this board.
ASIC — Application-Specific Integrated Circuit

The application-specific integrated circuit (ASIC) includes all digital logic for
the core processor functions and also for module-specific functions.
Motor Drive
The drive comprises motor control and motor amplifier.
Electromechanical Proportional Valve.

The EMPV consists of an electro-magnetic solenoid, driven by a PWM (Pulse
Width Modulator).
Pressure Converter
This block comprises a filter and amplifier for the pressure-sensor-signal, an
A/D converter with integrated multiplexer.
Leak Converter
This block comprises a PTC for the leak identification and a NTC for the
ambient temperature measurement. This assures that temperature changes
are not identified as leak. A leak would cool down the PTC and its change in
resistance results in a leak signal.
Fan Drive
The revolution of the fan is controlled by the main processor depending on
the internal heat distribution in the preparative pump. The fan provides a
PWM signal which is proportional to the revolution. This fan status signal is
used for diagnostics.
Seal Wash Pump Drive

The drive comprises a current control and full bridge driver for the seal wash
pump.
201
The Electronics
Pump head ID Module

The pump head ID Module is used to write pump head specific information
onto a tag that is installed in the pump head.
Index Board
The Index board is used to sense the movement and positioning of the pump
drive. It also comprises calibration information of the drive.
Electronic Fuses
The valve circuit is electronically fused on the board. Any error on the board
or shortages of the valve will activate the electronic fuse that will switch off
the supply voltage. This prevents the damage of components.
Onboard Battery
An onboard lithium battery buffers the electronic memories when the
preparative pump is turned off.
For safety information on lithium batteries, see “Lithium Batteries
Information” on page 254.
Interfaces
The preparative pump provides the following interfaces:
• two CAN connectors as interface to other Agilent 1100 Series modules,
• one RS-232C as interface to a computer,
• one REMOTE connector as interface to other Agilent products,
• one optional interface board.
• two CAN-DC-out for external CAN devices like a switch valve,
• LAN.
202
The Electronics
Figure 50 Block Diagram Pump Controller Board
to A/D Converter
1 (see next page)
Encoder
Optocoupler
Pump head Motor Drive J
Identification tag Index EEPROM

Comparator
Transceiver (TAG)
2 to A/D Converter
Processor
(see next page)
FPGA
Seal wash pump Drive Control
ASIC
EMPV (el. magn.

Valve drive
purge valve)
CAN-DC OUT
ON/OFF
CAN-DC OUT
3 to ADC (see next page)
203
The Electronics
Figure 51 Block Diagram Pump Controller Board
(see previous page)

1 2 3
Filter & Pressure

A/D converter Multiplexer sensor
amplifier
Processor
ASIC
Amplifier &
ambient meas. Leak sensor
Drive Fan
Sensor
Control Signal
Data/Address
Diagnose signals
ϑ
204
The Electronics
Figure 52 Interconnection Diagram CSM Board
Encoder P28 5V ASIC

SMF fuse
P18 +24Vm
Motor U+ 5V 10A +24V
Drive +24VCAN 3A P10 Power
U- +24VF
P19 +24VF 3A Supply
Lambda
P13 10A/24V/
+5V/2A 250W
+/-15V/0.2A
5V
Index P17
EEPROM Processor Optocoupler
+24VF
P8 Power
Switch
+24VF Status
P22 Electronic Hardware +24VF
EMPV Valve drive
fuse switch
Seal P23 Seal wash +24VF

wash pump drive 5V
+15V Leak P27 Leak

converter sensor
Pump head P20 +24VF P30

Control Fan drive Fan
ID (Tag) 5V
+/-15V Pressure P29 Pressure

converter sensor
-12V
P2
CAN P4, P5 CAN-DC OUT
RS232 P6 Power
+24VCAN
Remote P7 switch
P3
MIO P25 CAN-DC OUT
205
Firmware Description
The firmware of the instrument consists of two independent sections:

• a non-instrument specific section, called ‘resident system’,
• an instrument specific section, called ‘main system’.
Resident System
This resident section of the firmware is identical for all Agilent 1100 series
modules. Its properties are:
• the complete communication capabilities (CAN, LAN and RS-232C),
• memory management,
• ability to update the firmware of the ‘main system’.
Main System
Its properties are:
• the complete communication capabilities (CAN, LAN and RS-232C),
• memory management,
• ability to update the firmware of the ‘resident system’.
In addition the main system comprises the instrument functions that are
divided into common functions like
• run synchronization via APG remote
• error handling,
• diagnostic functions and so on,
or module specific functions like
• internal events such as motor control, flow rates and so on,
• calculation of compensation values for compressibility.
206
Firmware Updates
Firmware updates can be done using your user interface:
• handheld control module with files from a PC-card or
• Agilent ChemStation with files from floppy disk
The file naming conventions are:
xxxxvvvv.DLB, where
xxxx is the product number, e.g. 1361 for the G1361A preparative pump), and
vvvv is the revision number, for example A402 is revision A.04.02.
For instructions refer to your user interface.
NOTE Update of main system can be done in the resident system only.
Update of the resident system can be done in the main system only.
Figure 53 Firmware Update Mechanism
main FW update
Resident System Main System
resident FW update
207
Optional Interface Boards
The Agilent 1100 Series modules have one optional board slot that allows to
add an interface board to the modules.
Table 27 Optional Interface Boards
Description Part Number
BCD Board G1351-68701

Fuse 250 mA (four are on the board) 2110-0004
LAN Board (see next page for details)
BCD Board
The BCD board provides a BCD output for the bottle number of the
Agilent 1100 Series autosampler and four external contacts. The external
contact closure contacts are relay contacts. The maximum settings are: 30 V
(AC/DC); 250 mA (fused). There are general purpose cables available to
connect the BCD output (see “BCD Cables” on page 183) and the external
outputs (see “Remote Cables” on page 178) to external devices.
Figure 54 Block Diagram BCD Board
Board
identification
RFI filter
12 BCD
Processor
BCD register
interface connector
Line driver
RFI filter
+ 250 mA
External External
contacts 4× contact
connector
208
LAN Board
The HP LAN board is actually an HP JetDirect card, which is a network
interface card used in HP printers.
NOTE One board is required per Agilent 1100 stack. If the Agilent 1100 stack
has a DAD, then the DAD MUST be the module used for LAN board
installation. If no DAD is present a pump should be used for the LAN
board installation.
NOTE The LAN board can only be used together with:
A DAD, VWD, standard Pumps and ALS with main board version G13XX-66520
and greater and ChemStation software revision A.06.01 or above. The
following modules can be used with main board version G13XX-66500 and
greater and ChemStation revisions as indicated:
FLD A.06.03 or above,
RID, MWD A.06.04 or above,
WPS A.08.03 or above,
Cap. LC System A.08.03 or above,
Prep. Pump A.08.04 or above.
The following boards can be used with the Agilent 1100 modules.
Table 28 LAN Boards
Agilent Order Number Supported networks
J4106A Ethernet/802.3, RJ-45 (10Base-T)

J4105A Token Ring/802.5, DB9, RJ-45 (10Base-T)
J4100A Fast Ethernet, Ethernet/802.3, RJ-45 (10/100Base-TX) + BNC
(10Base2)
NOTE Minimum firmware of the LAN boards is A.05.05.
209
Recommended Cables
For point to point connection (not using a network hub) use a twisted pair
cross over LAN cable (P/N 5183-4649, 10 feet long).
For standard network connections using a hub use category 5 UTP cables,
(P/N G1530-61480, 8 m long).
210
Agilent 1100 Series Interfaces
The Agilent 1100 Series modules provide the following interfaces:
Table 29 Agilent 1100 Series Interfaces
DA Detector Thermostatted
Well Plate MW Detector VW Detector Column Vacuum
Interface Type Pumps Autosampler Sampler FL Detector RI Detector Compartment Degasser
CAN Yes Yes Yes Yes Yes Yes No

GPIB Yes/No* Yes No** Yes Yes Yes No
RS-232C Yes Yes Yes Yes Yes Yes No

Remote Yes Yes Yes Yes Yes Yes Yes
Analog Yes/No*** No No 2× 1× No Yes****
Interface board Yes Yes Yes Yes Yes No No
*
No for the G1361A Preparative Pump
**
The well plate sampler can be controlled through CAN via a detector that is connected via GPIB to a ChemStation.
*** No for the G1361A Preparative Pump
****
The vacuum degasser will have a special connector for specific use. For details see description of main board.
• The CAN connectors serve as the interface between the Agilent 1100
Series modules themselves,
• the GPIB connector serves as the interface between an Agilent1100
module and the Agilent ChemStation,
• RS-232C provides an interface to a computer,
• the APG remote connector serves as the interface between the
Agilent 1100 and other Agilent (APG remote compatible) products,
• the analog output connector(s) provide an analog signal output,
• the interface board slot (not common to all modules) provides specific
interfacing needs (external contacts, BCD, LAN and so on).
For identification and location of the connectors see Figure 31 on page 139.
211
regulations, see “Cable Overview” on page 174.
CAN Interface
The CAN is an inter module communication interface. It is a 2 wire serial bus
system supporting high speed data communication and real-time
requirements.
APG Remote Interface

The APG Remote connector may be used in combination with other
analytical instruments from Agilent Technologies if you want to use features
as common shut down, prepare, and so on.
Remote control allows easy connection between single instruments or
systems to ensure coordinated analysis with simple coupling requirements.
The subminiature D connector is used. The preparative pump provides one
remote connector which is inputs/outputs (wired-or technique).
To provide maximum safety within a distributed analysis system, one line is
dedicated to SHUT DOWN the system’s critical parts in case any module
detects a serious problem. To detect whether all participating modules are
switched on or properly powered, one line is defined to summarize the
POWER ON state of all connected modules. Control of analysis is maintained
by signal readiness READY for next analysis, followed by START of run and
optional STOP of run triggered on the respective lines. In addition PREPARE
and START REQUEST may be issued. The signal level are defined as:
• standard TTL levels (low = 0.0 - 0.8 V, high = +2.0 - 5 V)
• fan-out is 10,
• input load is 1.7 kOhm against + 5 V, and
• output are open collector type, inputs/outputs (wired-or technique).
212
Table 30 APG Remote Signal Distribution
Pin Signal Description
1 DGND Digital ground

2 PREPARE (L) Request to prepare for analysis (for example, detector lamp
on). Receiver is any module performing preanalysis activities.
3 START (L) Request to start run / timetable. Receiver is any module
performing run-time controlled activities.
4 SHUT DOWN (L) System has serious problem (for example, leak: stops pump).
Receiver is any module that needs to be shutdown to avoid a
safety risk.
5 Not used
6 POWER ON (H) All modules connected to system are switched on. Receiver is
any module relying on operation of others.
7 READY (H) System is ready for next analysis. Receiver is any sequence
controller.
8 STOP (L) Request to reach system ready state as soon as possible (for
example, stop run, abort or finish and stop injection). Receiver is
any module performing run-time controlled activities.
9 START REQUEST (L) Request to start injection cycle. Receiver is the autosampler.
213
RS-232C
The RS-232C connector is used to control the instrument from a computer
through RS-232C connection, using the appropriate software. This connector
can be configured with the configuration switch module next to the CAN-DC
OUT connector. The RS-232C is designed as DCE (data communication
equipment) with a 9-pin male SUB-D type connector. The pins are defined as:
Table 31 RS-232C Connection Table
Pin Direction Function
1 In DCD
2 In RxD
3 Out TxD
4 Out DTR
5 Ground
6 In DSR
7 Out RTS
8 In CTS
9 In RI
Figure 55 RS-232 Cable
Instrument PC
DCD 1 1 DCD
RX 2 2 RX
TX 3 3 TX
DTR 4 4 DTR
GND 5 5 GND
DSR 6 6 DSR
RTS 7 7 RTS
CTS 8 8 CTS
RI 9 9 RI
DB9 DB9 DB9 DB9

Male Female Female Male
214
Setting the 8-bit Configuration Switch
The 8-bit configuration switch is located in the upper right corner of the rear
of the instrument, next to the CAN-DC OUT connectors. Switch settings
provide configuration parameters for serial communication protocol and
instrument specific initialization procedures.
The factory default setting for the preparative pump is:
all switches set to 0 (down).
Figure 56 8-bit Configuration Switch
Factory setting is shown

for preparative pump
Table 32 8-bit Configuration Switch
Mode Select 1 2 3 4 5 6 7 8
RS-232C 0 1 Baudrate Data Bits Parity

Reserved 1 0 Reserved
TEST/BOOT 1 1 RSVD SYS RSVD RSVD FC
Switches 1 and 2 define which set of parameters (for example, for RS-232C)
will be changed. Once the change has been completed, the instrument must
be powered up again in order to store the values in the non-volatile memory.
215
In the non-volatile memory the parameters are kept, regardless of whether

you turn the instrument off and on again. They will be kept until the same set
of parameters is subsequently changed and power is reset. All other
previously stored configuration settings will still remain in the non-volatile
memory.
Communication Settings for RS-232C Communication

The communication protocol used in the instrument supports only hardware
handshake (CTS/RTR).
Switches 1 in down and 2 in up position define that the RS-232C parameters
will be changed. Once the change has been completed, the instrument must
be powered up again in order to store the values in the non-volatile memory.
Use the following tables for selecting the setting which you want to use for
RS-232C communication. The number 0 means that the switch is down and 1
means that the switch is up.
Table 33 Communication Settings for RS-232C Communication
Mode Select 1 2 3 4 5 6 7 8
RS-232 0 1 Baudrate Data Bits Parity
Use the following tables for selecting the setting which you want to use for
RS-232C communication. The number 0 means that the switch is down and 1
means that the switch is up
Table 34 Baudrate Settings
Switches Baud Rate Switches Baud Rate
3 4 5 3 4 5
0 0 0 9600 (default) 1 0 0 9600

0 0 1 1200 1 0 1 14400
0 1 0 2400 1 1 0 19200
0 1 1 4800 1 1 1 38400
216
Table 35 Data Bit Settings
Switch 6 Data Word Size
0 7 Bit Communication
1 8 Bit Communication
One start bit and one stop bit are always used (not selectable).
Per default, the module will turn into 19200 baud, 8 data bit with no parity.
Table 36 Parity Settings
Switches Parity
7 8
0 0 No Parity
0 1 Odd Parity
1 0 Even Parity
Forced Cold-Start Settings

Switches 1 and 2 do not force storage of this set of parameters in non-volatile
memory. Returning the switches 1 and 2 to other positions (other than being
both up) will allow for normal operation.
CA UT IO N A forced cold start erases all methods and data stored in non-volatile memory.
Exceptions are diagnose and repair logbooks which will not be erased.
217
If you use the following switch settings and power the instrument up again, a
forced cold start has been completed.
Table 37 Forced Cold Start Settings
Mode Select 1 2 3 4 5 6 7 8
TEST/BOOT 1 1 0 0 0 0 0 1
To return to normal operation, set switches back to your GPIB or RS 232C

configuration settings.
Stay Resident Settings

Firmware update procedures may require this mode in case of firmware
loading errors.
Switches 1 and 2 do not force storage of this set of parameters in non-volatile
memory. Returning the switches 1 and 2 to other positions (other than being
both up) will allow for normal operation.
If you use the following switch settings and power the instrument up again,
the instrument firmware stays in the resident part, that is, it is not operable as
a preparative pump. It only uses basic functions of the operating system, for
example, for communication, and so on.
Table 38 Stay Resident Settings
Mode Select 1 2 3 4 5 6 7 8
TEST/BOOT 1 1 0 0 1 0 0 0
To return to normal operation, set switches back to your RS 232C

configuration settings.
218
The Main Power Supply Assembly
The main power supply comprises a closed assembly (no component-level

repair possibility).
The power supply provides 24 DC voltage used in the preparative pump
module. The line voltage can vary in a range from 100 – 240 volts AC ± 10 %
and needs no manual setting.
WAR NI N G To disconnect the instrument from line, unplug the power cord. The
power supply still uses some power, even if the power switch on the
front panel is turned off.
No accessible hardware fuse is needed because the main power supply is

safe against any short circuits or overload conditions on the output lines.
When overload conditions occur, the power supply turns off the output
voltage, until the cause of the overload condition has been removed.
An over-temperature sensor in the main power supply is used to turn off
output voltages if the temperature exceeds the acceptable limit (for example,
if the cooling fan of the preparative pump fails). To reset the main power
supply to normal operating conditions, turn the preparative pump off, wait
until it is approximately at ambient temperature and turn the preparative
pump on again.
The following table gives the specifications of the main power supply.
Table 39 Main Power Supply Specifications
Maximum power 250 VA Continuous output

Line Input 100 – 240 volts AC ± 10 %, Wide ranging
line frequency of 50 - 60 Hz ± 5.0 %
Output + 24 V
219
220
7
7 Control Module Screens

for the Preparative Pump
Introduction to the screens available for
operation of the Agilent 1100 Series preparative
pump with the control module
Control Module Screens for
the Preparative Pump
This chapter is intended to introduce an operator to the functionality

available for operation of the Agilent 1100 preparative pump with the
Agilent 1100 control module.
Please use the manual of the control module for further detailed reference.
Major keys on the Agilent 1100 Control Module
ESC Return to previous screen, abort any change of parameters and

toggle between the last two top layer views
m Open context sensitive menus
i Information/Help
Enter Store changed parameters or execute a choice
Done (If available) Activate settings of current screen
On/Off Switch on individual Instrument(s) or complete System
Start Start a location range or sequence
Plot View online signals
Views Change between analysis - (samples)- status - system views
NOTE The screens shown on the next pages are based on the following firmware
revisions:
Control Module firmware revision B.02.0x (G1323B).
HPLC Module firmware revision 4.1x.
NOTE In case the control module’s display seems to be frozen (hang-up due to a
communication problem on the CAN bus) unplug the control module from the
HPLC module and reconnect.
222
Control Module Screens for the Preparative Pump
Screens available from the Analysis view
Analysis view This is the wake-up screen, if the Agilent 1100 preparative pump is the only
configured Agilent 1100 module. It is used to enter the most common pump
method parameters.
The m-key allows access to the context sensitive menus. Date&Time allows
you to change time settings. Print Screen gives access to the print screen.
About tells you the current firmware revision and the serial# of your control
module. Setup view leads you to analysis view configuration for additional
Agilent 1100 modules. Restart re-boots the control module.
223
Setup View In the Setup view, modules can be added or removed to the analysis view.
Select a module, you want to move, with the help of the selection keys. Use
function keys F7/F8 (Remove/Add) to move the highlighted module.
Changes must be activated with Done (F6).
Here, e.g. in addition to a binary preparative pump, the thermostatted column

compartment and diode array detector parameters are shown on the display
as well. The number of parameters for each module is reduced as additional
modules are added to the display. A maximum of 4 modules can be shown
simultaneously (If more modules are connected to the system, you can
choose up to 4 of them in Setup view).
224
On/Off From the Analysis view use the F7-key (On/Off) to proceed to the Turn On
screen. Press F8 (On) once to turn on the pump. If more than one module is
available, select F8 (Pump) or turn on the entire system by selecting the
System On button and pressing Enter.
Settings With the F1-key (Settings) you open a pull-down menu where you can select
the preparative pump module with the help of the selection or
alphanumeric keys.
225
Settings In the Settings screen you can change the pump parameters. Here, you can
enter values for the flow or composition (for gradient prep. pump, only). In
addition, you have access to a different set of parameters available through
the F1-5 navigation-keys. The F7-key (Default) resets the pump to default
values. F8 (On/Off) opens a window to turn on the pump. The following
figures show the screens for an isocratic preparative pump or a gradient
preparative pump. Changes must be acknowledged with F6 (Done).
Use the m-key for the context sensitive menu. Reset will load the pump
default parameters. The Status command pulls up the module specific setup
screen.
226
Use the F1-key (More) to enter special pump setpoints:
Settings - More... Select Flow Ramp / Compr. to enter appropriate for the flow ramp up speed
and the solvent compressibility (see “How to Optimize the Compressibility
Compensation Setting” on page 47). Changes must be activated with F6
(Done).
Select Automatic Purge to enter a purge time (EMPV opened). Changes

must be activated with F6 (Done).
227
Settings - Timetable With the F2-key (Timetable) you can list and edit the timetable for the
pump. Press F7-key (Insert) to add entries, F6-key (Delete) to remove or
Enter to edit selected/highlighted entries .
Edit the different sections of each timetable line. Use the pull-down menus, if
available. Use the F6-key (Done) to view the entered lines of the timetable or
F7 (Enter) to acknowledge current settings.
Use the m-key for the context sensitive menu. It gives you additional tools for
the timetable.
228
Settings - Pressure With the F3-key (Pressure) you can change the settings for the pressure
limits.
Settings - Bottle With the F4-key (Bottle Fillings) you can adjust the settings for the bottle
Fillings fillings to their current state. Select F8 (Refill) to refill All bottles or a
Selected bottle to total volume. By selecting the respective tick-boxes, you
can also choose whether and when to have Not-Ready or Error conditions.
The following figures show the screens for an a binary gradient preparative
pump. Changes must be activated with F6 (Done).
229
Settings - Runtimes With the F5-key (Runtimes) you can change the stop time and the post-run
time for this module individually. Changes must be activated with F6 (Done).
Analysis -Status Press F5-key (Views) and select Status.
Status View This is an example if an Agilent 1100 binary gradient preparative pump is
configured standalone. Information on the actual flow rate, mobile phase
composition, pressure and %-Ripple, elapsed run time and the pressure plot
are shown. Press key F8 (Start) to start a run, key F7 (Rescale) to
maximize the signal.
230
This is a Status view for multiple instruments:
231
Signal plot Press F6-key (Plot) to enter the plot screen (available also from all other
views). Here you can observe online signal(s). If more than one signal is
configured, use the 1-2-3 numeric keys to switch between the signals.
The m-key allows you to Clear All signals, Maximize signals and Print the
plot.
Press F6 (Select) to configure online signals (maximum are 3). Additional

signals can also be chromatograms or temperature signals from other
modules. Use the Right/Left selection keys to switch between ‘Available
Signals’ and ‘Selected Signals’. Use the F8-key (Move) or Enter to move
available signals into the box for selected signals or vice versa. Use F7 to
Setup ranges for each individual (highlighted) signal. Press F6 (Done) to
activate the changed settings and to return to the Plot screen.
232
Method screens On the Analysis view use the F3-key (Method) to view the parameters in a
method. Use the F8-key (Save As) to save the method in the module(s). The
PC-Card-key (F2) is only active when a PCMCIA card is inserted in the
control module.
NOTE The PCMCIA card is only recognized, if inserted before last start-up.
Use F2-key (PC-Card) to transfer a method from/to a PCMCIA card. Use the
Right/Left selection keys to switch between PC-Card and Instrument
window. Use the Up/Down selection keys to select the method. Use the
F7/F8-keys (Copy) or Enter to move available methods between PCMCIA
card and Modules. F6 Deletes a selected method.
233
Screens available from the System view
System view Use the Esc-key to until you receive Views on the F5-key. Choose System
from the pull-down menu. This screen shows the last activities in the system.
System - Control Use the F1-key (Control) and select the preparative pump.
234
In the preparative pump’s Control screen, you can turn On or Off (F8) the
pump, or Reset (F7) the pump. Here you receive information about the
not-ready conditions if needed.
Use F1 (Purge Task) to access the pump’s Purge Task. After selecting a
channel (gradient system, only), you can edit its Purge Time. Changes must
be activated with F6 (Done).
Press F2 (Seal wash) in the ‘System/Control’ screen to configure the seal

wash pump. You can turn Off the seal wash pump, define a Single Wash off a
certain Duration or setup Periodic wash cycles with certain wash Periods
and a %- value for the overall seal wash pump ON time (should never exceed
30%).
235
System - On the System view use the F2-key (Configure) and select the pump.
Configuration
This screen gives you information about installed options and allows you to
configure your pump to the filter and mixer type that is actually installed in
the pump. Changes must be activated with F6 (Done).
Enter Baudrate, Bits and Parity to configure your serial interface.
236
Press F5 (Gradient Pump) in the Config screen. Here you can configure,
which of the two isocratic preparative pumps that form a gradient
preparative pump represents channel A, which represents channel B. In case
you are not sure, you can press F8 (Identify) to identity, which pump
represents which channel. The status indicator of the respective instrument
will then start to blink. By pressing F7 (Break) you can separate the two
combined pumps and use each of them as an individual module. Changes
must be activated with Done (F6).
237
Screens available from the Records screen
Records screen Use the Esc-key until you receive Views on the F5-key. Choose System from
the pull-down menu. Use the F4-key (Records) and select the pump. Errors
are reported either into the F2 (System Log) or F3 (Error Log).
EMF (Early Use the F1-key (EMF) to set EMF Limits. Choose menu item Setup Limits
Maintenance to set the amount of pumped solvent or seal wear, when you want to receive
Feedback) a warning for exceeded limits.
238
Press F7 (Reset) to reset the counters after exchanging parts that are
subject to wear. Changes must be activated with F6 (Done).
If a set limit has been exceeded, a message box will pop up.
NOTE An exceeded limit will not stop a sequence or run (information only, to plan
maintenance activities).
If you press Reset, the limits will be removed. Ignore will continue to keep
the EMF flag set.
System / Error Log Use the F2-key (System Log) or F3-key (Error Log) to look for errors on
the selected/ highlighted module.
239
Maintenance Log Use the F4-key (Maint Log) to review and enter maintenance activities in
the maintenance logbook.
Use the F7-key (Add) to add new maintenance activities. You can add a
comment to the activity into the line ‘Add’ using the control modules
alphanumeric keys. Changes must be activated with F6 (Done).
240
Firmware Update Use the Esc-key to receive Views on the F5-key. Choose System from the
pull-down menu. Use the F3-key (Records) to select the pump. Use the
F5-key (FW Update) to enter the Update section. If you want to update the
resident firmware (together with specific main firmware revisions), select
the file from the PCMCIA card (RESnnnn.DLB) and press execute. If you
want to update the main firmware, press F7-key (Transfer) to turn the
module into the resident mode (LED on module should blink yellow).
Use the Esc-key to receive Views on the F5-key. Choose System from the
pull-down menu. Use the F3-key (Records) to select the Generic module. In
this screen the resident firmware revision is shown.
241
Use the F5-key (FW Update) to enter the Update section. Select the a file
from the PCMCIA card (1361nnnn.DLB) and press execute.
If you have not saved your methods, please do it before continuing.

Otherwise they will be overwritten during the update process.
When the update has finished, press F7-key (Transfer) to return the module
into the normal mode (LED on module should stay yellow).
242
Changing the serial In case the serial number of the module has to be added, use the m-key to
number open the menu Enter Serial#. The serial number becomes active after
restart of the module.
Type in the module’s Serial# as indicated on the front or rear label of the
module using the alphanumeric keys. Changes must be activated with F6
(Done).
243
Diagnostics and Tests
Diagnostics and Tests
Tests screen Use the Esc-key until you receive Views on the F5-key. Choose System from
the pull-down menu. Use the F3-key (Tests) to select the pump.
Leak Test Press F2 (Leak Test) on the Test screen to perform a leak test. Several steps
like purging the system, setting up Isopropanol as solvent and blocking the
purge valve outlet with a blank nut have to be performed before operating the
leak test. For details use the i-key to achieve context sensitive help, follow
the instructions on the screen and refer to “Running the Leak Test” on page
102. For evaluating the leak test refer to “Evaluating the Results” on page 103.
Pressure Test Use the F3-key (Pressure Test) to perform a pressure test of the system.
Several steps like purging the system, setting up Isopropanol as solvent and
blocking the column outlet with a blank nut have to be performed before
operating the pressure test. For details use the i-key to achieve context
sensitive help, follow the instructions on the screen and refer to “Running the
Pressure Test” on page 95. For evaluating the pressure test refer to
“Evaluating the Results” on page 97.
244
8
8 Specifications
Performance specifications of the preparative

pump
Specifications
Performance Specifications
Performance Specifications
Table 40 Performance Specification Agilent 1100 Series Preparative Pump
Type Specification
Hydraulic system Dual pistons in parallel

Settable flow range 0.001 – 100 ml/min
Flow precision < 0.5 % RSD
Pressure range 20 to 400 bar (5880 psi) system pressure
Compressibility User-selectable, based on mobile phase compressibility
compensation
Recommended pH range 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack
stainless steel.
Control and data Agilent ChemStation for LC
evaluation
Communications Controller-area network (CAN), RS-232, APG Remote: ready, start, stop and
shut-down signals, CAN-DC OUT, LAN optional
Safety and maintenance Extensive diagnostics, error detection and display (through control module
and Agilent ChemStation), leak detection, safe leak handling, leak output
signal for shutdown of pumping system. Low voltages in major
maintenance areas.
GLP features Early maintenance feedback (EMF) for continuous tracking of instrument
usage in terms of seal wear and volume of pumped mobile phase with
user-settable limits and feedback messages. Electronic records of
maintenance and errors.
Housing All materials recyclable.
246
Legal, Safety and Warranty
Information
Warranty Statement
Warranty Statement
All Chemical Analysis Products

Agilent Technologies warrants its chemical analysis products against defects in
materials and workmanship. For details of the warranty period in your country, call
Agilent. During the warranty period, Agilent will, at its option, repair or replace
products which prove to be defective. Products that are installed by Agilent are
warranted from the installation date, all others from the ship date.
If buyer schedules or delays installation more than 30 days after delivery, then
warranty period starts on 31st day from date of shipment (60 and 61 days,
respectively for products shipped internationally).
Agilent warrants that its software and firmware designed by Agilent for use with a
CPU will execute its programming instructions when properly installed on that
CPU. Agilent does not warrant that the operation of the CPU, or software, or
firmware will be uninterrupted or error-free.
Limitation of Warranty
Onsite warranty services are provided at the initial installation point. Installation and
onsite warranty services are available only in Agilent service travel areas, and only
in the country of initial purchase unless buyer pays Agilent international prices for
the product and services. Warranties requiring return to Agilent are not limited to the
country of purchase.
For installation and warranty services outside of Agilent’s service travel area,
Agilent will provide a quotation for the applicable additional services.
If products eligible for installation and onsite warranty services are moved from the
initial installation point, the warranty will remain in effect only if the customer
purchases additional inspection or installation services, at the new site.
The foregoing warranty shall not apply to defects resulting from:
1 improper or inadequate maintenance, adjustment, calibration, usage of corrosive
solvent as described in the solvent information note later in this chapter or
operation by buyer,
2 buyer-supplied software, hardware, interfacing or consumables,
3 unauthorized modification or misuse,
248
Warranty Statement
4 operation outside of the environmental and electrical specifications for the

product,
5 improper site preparation and maintenance, or
6 customer induced contamination or leaks.
THE WARANTY SET FORTH IS EXCLUSIVE AND NO OTHER WARRANTY,
WHETHER WRITTEN OR ORAL, IS EXPRESSED OR IMPLIED. AGILENT
SPECIFICALLY DISCLAIMS THE IMPLIED WARRANTIES OF
MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE.
Limitation of Remedies and Liability

THE REMEDIES PROVIDED HEREIN ARE BUYER’S SOLE AND
EXCLUSIVE REMEDIES. IN NO EVENT SHALL AGILENT BE LIABLE FOR
DIRECT, INDIRECT, SPECIAL, INCIDENTAL, OR CONSEQUENTIAL
DAMAGES (INCLUDING LOSS OF PROFITS) WHETHER BASED ON
CONTRACT, TORT OR ANY OTHER LEGAL THEORY.
Responsibilities of the Customer

The customer shall provide:
1 access to the products during the specified periods of coverage to perform
maintenance,
2 adequate working space around the products for servicing by Agilent personnel,
3 access to and use of all information and facilities determined necessary by
Agilent to service and/or maintain the products (insofar as these items may
contain proprietary or classified information, the customer shall assume full
responsibility for safeguarding and protection from wrongful use),
4 routine operator maintenance and cleaning as specified in the Agilent operating
and service manuals, and
5 consumables such as paper, disks, magnetic tapes, ribbons, inks, pens, gases,
solvents, columns, syringes, lamps, septa, needles, filters, frits, fuses, seals,
detector flow cell windows, and so on.
249
Warranty Statement
Responsibilities of Agilent Technologies

Agilent Technologies will provide warranty services as described in Table 41.
Table 41 Warranty Services
Services During Warranty* Warranty Period** Type
Agilent 1100 Series of Modules 1 Year Onsite

GC, LC, UV-Visible, and LAS supplies and 90 Days Onsite
accessories
Columns and Consumables*** 90 Days Return to

Agilent
Gas Discharge and Tungsten Lamps 30 Days Return to
Agilent
Repairs performed on-site by Agilent**** 90 Days Onsite
*
This warranty may be modified in accordance with the law of your country. Please consult your
local Agilent office for the period of the warranty, for shipping instructions and for the applicable
wording of the local warranty.
**
Warranty services are included as specified for Analytical products and options purchased
concurrently provided customer is located within a Agilent defined travel area. Agilent warranty
service provides for 8 a.m. to 5 p.m. on-site coverage Monday through Friday, exclusive of Agilent
holidays.
*** Columns and Consumables are warranted to be free from defects for a period of 90 days after
shipment and will be replaced on a return-to-Agilent basis if unused.

****
Agilent repair warranty is limited to only the item repaired or replaced.
250
Safety Information
Safety Information
The following general safety precautions must be observed during all phases of
operation, service, and repair of this instrument. Failure to comply with these
precautions or with specific warnings elsewhere in this manual violates safety
standards of design, manufacture, and intended use of the instrument. Agilent
Technologies assumes no liability for the customer’s failure to comply with these
requirements.
General
This is a Safety Class I instrument (provided with terminal for protective earthing)
and has been manufactured and tested according to international safety standards.
Operation
Before applying power, comply with the installation section. Additionally the
following must be observed.
Do not remove instrument covers when operating. Before the instrument is switched
on, all protective earth terminals, extension cords, auto-transformers, and devices
connected to it must be connected to a protective earth via a ground socket. Any
interruption of the protective earth grounding will cause a potential shock hazard
that could result in serious personal injury. Whenever it is likely that the protection
has been impaired, the instrument must be made inoperative and be secured against
any intended operation.
Make sure that only fuses with the required rated current and of the specified type
(normal blow, time delay, and so on) are used for replacement. The use of repaired
fuses and the short-circuiting of fuseholders must be avoided.
WAR NI N G Any adjustment, maintenance, and repair of the opened instrument

under voltage is forbidden.
WAR NI N G Disconnect the instrument from the line and unplug the power cord
before maintenance.
251
Safety Information
Do not operate the instrument in the presence of flammable gases or fumes.

Operation of any electrical instrument in such an environment constitutes a definite
safety hazard.
Do not install substitute parts or make any unauthorized modification to the
instrument.
Capacitors inside the instrument may still be charged, even though the instrument
has been disconnected from its source of supply. Dangerous voltages, capable of
causing serious personal injury, are present in this instrument. Use extreme caution
when handling, testing and adjusting.
Safety Symbols
Table 42 shows safety symbols used on the instrument and in the manuals.
Table 42 Safety Symbols
Symbol Description
The apparatus is marked with this symbol when the user should refer
! to the instruction manual in order to prevent risk of harm to the
operator and to protect the apparatus against damage.
Indicates dangerous voltages.
Indicates a protected conductor terminal.
Eye damage may result from directly viewing the light produced by
the Xenon flash lamp used in this product. Always turn the xenon flash
lamp off before removing it.
WAR NI N G A warning alerts you to situations that could cause physical injury or
damage to the equipment. Do not proceed beyond a warning until you
have fully understood and met the indicated conditions.
252
Safety Information
CA UT IO N A caution alerts you to situations that could cause a possible loss of data. Do
not proceed beyond a caution until you have fully understood and met the
indicated conditions.
253
Lithium Batteries Information
Lithium Batteries Information
WAR NI N G Danger of explosion if battery is incorrectly replaced. Replace only

with the same or equivalent type recommended by the equipment
manufacturer. Lithium batteries may not be disposed-off into the
domestic waste.
Transportation of discharged Lithium batteries through carriers

regulated by IATA/ICAO, ADR, RID, IMDG is not allowed. Discharged
Lithium batteries shall be disposed off locally according to national
waste disposal regulations for batteries.
WAR NI N G Lithiumbatteri - Eksplosionsfare ved fejlagtic handtering. Udskiftning

ma kun ske med batteri af samme fabrikat og type. Lever det brugte
batteri tilbage til leverandoren.
WAR NI N G Lithiumbatteri - Eksplosionsfare. Ved udskiftning benyttes kun

batteri som anbefalt av apparatfabrikanten. Brukt batteri returneres
appararleverandoren.
NOTE Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet
weggooien maar inleveren als KCA.
254
Radio Interference
Radio Interference
Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
Test and Measurement

If test and measurement equipment is operated with equipment unscreened cables
and/or used for measurements on open set-ups, the user has to assure that under
operating conditions the radio interference limits are still met within the premises.
Sound Emission
Manufacturer’s Declaration
This statement is provided to comply with the requirements of the German Sound
Emission Directive of 18 January 1991.
This product has a sound pressure emission (at the operator position) < 70 dB.
• Sound Pressure Lp < 70 dB (A)
• At Operator Position
• Normal Operation
• According to ISO 7779:1988/EN 27779/1991 (Type Test)
255
Solvent Information
Solvent Information
Observe the following recommendations on the use of solvents.
Solvents
Brown glass ware can avoid growth of algae.
Always filter solvents, small particles can permanently block the capillaries. Avoid
the use of the following steel-corrosive solvents:
• Solutions of alkali halides and their respective acids (for example, lithium iodide,
potassium chloride, and so on).
• High concentrations of inorganic acids like nitric acid, sulfuric acid especially at
higher temperatures (replace, if your chromatography method allows, by
phosphoric acid or phosphate buffer which are less corrosive against stainless
steel).
• Halogenated solvents or mixtures which form radicals and/or acids, for example:
2CHCl3 + O2 → 2COCl2 + 2HCl
This reaction, in which stainless steel probably acts as a catalyst, occurs quickly
with dried chloroform if the drying process removes the stabilizing alcohol.
• Chromatographic grade ethers, which can contain peroxides (for example, THF,
dioxane, di-isopropylether) such ethers should be filtered through dry aluminium
oxide which adsorbs the peroxides.
• Solutions of organic acids (acetic acid, formic acid, and so on) in organic
solvents. For example, a 1-% solution of acetic acid in methanol will attack steel.
• Solutions containing strong complexing agents (for example, EDTA, ethylene
diamine tetra-acetic acid).
• Mixtures of carbon tetrachloride with 2-propanol or THF.
256
Agilent Technologies on Internet
For the latest information on products and services visit our worldwide web site on
the Internet at:
http://www.agilent.com
Select “Products” - “Chemical Analysis”
It will provide also the latest firmware of the Agilent 1100 series modules for
download.
257
258
Index
Numerics connecting APG remote, 23, 25, 27 E

24V supply for externa ldevices low, 91 connecting CAN, 23, 25, 27 electrical connections, 30, 195
connecting GPIB, 23, 25, 27 electromagnetic proportioning valve,
A connecting LAN, 23, 25, 27 201
adapter, 32, 35 connecting the ChemStation, 23, 25, electronic fuses, 202
additional interface and control cables, 27 electronics
29 connecting the power, 23, 25, 27 PPM board, 200
Agilent on internet, 257 external contacts, 175, 186 electrostatic discharge (ESD), 111
air circulation, 15 GPIB, 175 EMF, 198
air flow, 143 overview, 174 on control module, 241
algae, 42, 44 remote, 174, 178 EMF counter, 198
ambient non-operating temperature, 16 cables, 15 EMF flag, 198
ambient operating temperature, 16 CAN bus, 30 EMF limits, 199
analog cable, 174, 176 CAN cable, 29, 185 EMPV, 114, 121, 201
APG remote connector, 212 CAN interface, 212 EMPV Cleaning, 51
aqueous buffer solutions, 46 CAN-DC-out, 30 EMPV cleaning procedure, 106
ASIC - application-specific integrated cleaning the pump, 112 EMPV fuse blown, 80
circuit, 201 compensation sensor open, 63 EMPV Missing, 81
assembling compensation sensor short, 64 EMPV parts, 164
main cover, 133, 150 composition cluster partner lost, 85 encoder not connected, 79
auxiliary cable, 175, 185 compressibility compensation, 47, 246 environment, 14, 15
condensation, 15 error condition, 53
B connections, 31 error messages, 50, 55
back pressure, 42 electrical, 30 24V supply for externa ldevices low,
basis kit, 17, 18, 169 connections, flow, 34 91
basis kit parts, 169 control and data evaluation, 246 compensation sensor open, 63
battery, 202 control module compensation sensor short, 64
safety information, 254 EMF, 241 composition cluster partner lost, 85
baudrate setting, 216 firmware update, 241 EMPV fuse blown, 80
BCD board, 30, 131, 208 serial number change, 243 EMPV Missing, 81
BCD cable, 174, 183 tests, 244 encoder not connected, 79
bench space, 15 control module parts, 171 fan failed, 65
blank nut, 95, 106 cover parts, 166 index missing, 76
block diagram, 203, 204 leak, 60
pump controller board, 203, 204 D leak sensor open, 61
blocking of filters, 42 damaged packaging, 17 leak sensor short, 62
board connectors, 138 damaged parts, 17 motor direction, 74
board layout, 139 data bit setting, 217 motor -driver fault, 72
bottle head assembly parts, 159 delay volume, 21 motor driver temperature too high,
buffer application, 42 delivery checklist 71
buffer solutions, 46 preparative pump, 18 motor maximum power fault, 73
details no motor motion, 87
C preparative pump, 193 no pump head, 82
cable dimensions, 16 open cover, 66
analog, 174, 176 disassembling position error, 77
auxiliary, 175, 185 pump head assembly, 114, 125 pressure above upper limit, 67
BCD, 174, 183 drive, 133 pressure below lower limit, 68
CAN, 185 pressure sensor not connected, 69
259
Index
pump off while analysis, 75 F hydraulic path parts, 160

remote time-out, 58 fan, 133, 143 hydraulic system, 246
seal wash, 89 air flow, 143
seal wash current low, 88 fan drive, 201 I
shut-down, 57 fan failed, 65 index board, 202
solvent counter zero, 86 features index missing, 76
spurious index, 78 GLP, 246 indicator, status, 52
synchronization lost, 59 instrument layout, 197 inlet valve, 115
time-out, 56 safety and maintenace, 246 inlet valves, 114
wait for encoder position timed out, filter cup, 114, 117 installation
90 filter plate, 114, 119 pump module, 28
wait for pressure timed out, 84 filters, 117, 119 installing the pump, 13
wait for volume timed out, 83 firmware instrument layout, 197
wrong data from pressure sensor, 70 description, 206 instrument status indicator, 53
ESD strap, 112 main system, 206 interconnection Diagram
exchanging resident system, 206 CSM board, 205
EMPV, 114, 121 updates, 207 interface
fan, 133, 143 firmware update with control module, remote, 212
filter, 114 241 RS-232C, 214
filter cup, 114, 117 first analysis, 38 interface board, 30, 131
filter plate, 114, 119 flow, 31 interfaces, 202
filters, 117, 119 flow connections, 31, 34 internal parts, 110, 132
inlet valve, 114, 115 gradient pump, 34 internet, 257
interface board, 131 isocratic pump, 31 introduction
internal parts, 110, 132 flow precision, 246 preparative pump, 190
leak sensor, 133, 146 flow range, 246 introduction to the pump, 190
light pipe, 133, 148 foam, 133, 134, 151 isocratic preparative pump, 28
main board, 133, 138 foam parts, 168
main cover, 133, 150 forced cold-start setting, 217 L
multi assembly, 133, 136 front cover, 31 lamp
outlet valve, 114, 115 front view instrument status, 53
pistons, 114, 125 isocratic preparative pump, 28 lamp, status, 52
plungers, 114, 125 fuse, 202 LAN
power supply, 133, 145 fuses, 14 recommended cables, 210
power switch, 133, 148 LAN cables, 188
PPM board, 133, 138 G LAN interface board, 209
pressure sensor, 133, 136 GPIB cable, 175 leak, 60
pump drive, 133, 144 gradient kit, 17, 20, 170 leak converter, 201
purge valve, 114, 121 gradient kit parts, 170 leak parts, 173
seal wash pump cassette, 114, 124 ground connection, 14 leak sensor, 133, 146
seal wash pump drive, 133, 147 grounding leak sensor open, 61
seals, 114, 125 metal bottles, 32 leak sensor short, 62
status light pipe, 133, 149 grounding of solvent bottle, 31 leak test, 50, 99
valves, 114, 115, 121 evaluation, 103
external contact cable, 175, 186 H leakage, 32
externally accessible fuses, 14 hints for successful use, 42 light pipe, 133, 148
housing, 246 light pipes, 172
humidity, 16 line frequency, 16, 30
260
Index
line input, 219 preparative pump, 191 preparative pump

line voltage, 30 overview of parts, 113 connections, 30
liquimeter, 198 preparative pump delivery checklist, 18
lithium batteries, 254 P preparative pump main board (PPM),
logbook, 56 parity setting, 217 201
particles, 43 prerun condition, 53
M parts pressure, 246
main assemblies parts, 156 basis kit, 169 pressure above upper limit, 67
main assemblies, overview, 113 bottle head assembly, 159 pressure below lower limit, 68
main board, 133, 201 control module, 171 pressure converter, 201
main cover, 133, 150 cover, 166 pressure pulsation, 47
main power supply assembly, 219 damaged, 17 pressure sensor, 133, 136
main power supply specification, 219 EMPV parts, 164 pressure sensor not connected, 69
maintaining foam, 168 pressure test, 50, 92
internal parts, 110 gradient kit, 170 priming, 38
pump drive, 144 hydraulic path, 160 priming solvents, 39
maintenance procedures leak handling, 173 pump
EMF, 198 light pipes, 172 installing, 13
Materials in contact with mobile phase, main assemblies, 156 unpacking, 17
193 missing, 17 pump drive, 133, 144
metal bottles, 32 multi assembly parts, 165 pump head assembly, 114, 125, 128, 162
missing parts, 17 power and status light pipes, 172 pump head ID module, 202
motor direction, 74 pump head, 162 pump housing parts, 156
motor drive, 201 pump housing, 156 pump main board, 133, 138
motor -driver fault, 72 sheet metal kit, 167 pump off while analysis, 75
motor driver temperature too high, 71 solvent cabinet, 159 purge valve, 114, 121
motor maximum power fault, 73 parts identification
multi assembly, 133, 136 cables - LAN cables, 188 R
multi assembly parts, 165 performance specification, 246 radio interference, 255
pH range, 246 reassembling
N physical specifications, 16 pump head assembly, 114, 128
no motor motion, 87 pistons, 114, 125 recommended pH range, 246
no pump head, 82 plungers, 42, 114, 125 remote
not-ready condition, 53 position error, 77 signal distribution, 213
power connections, 29 remote cable, 174, 178
O Power Consideration, 14 REMOTE connector, 30
onboard battery, 202 power considerations, 14 remote interface, 212
open cover, 66 power consumption, 16, 30 remote time-out, 58
operating Altitude, 16 power cords, 14 removing
operating range, 246 power input, 30 foam, 133, 134
optimizing performance, 41 power light pipe, 172 pump head assembly, 114, 125
optimum performance, 21 power parts, 172 top cover, 133, 134
optional interface boards, 208 power supply, 133, 145, 219 repair procedures, 114
organic solvents, 46 wide-ranging capability, 14, 30 repairing
outet valve, 115 power supply indicator, 53 inlet valve, 114
outlet valves, 114 power supply specification, 219 internal parts, 110
overview power switch, 28, 133, 148 outlet valve, 114
hydraulic path, 192 PPM board, 133, 138, 200 valves, 114
261
Index
replacing RS-232C communication, 216 W

foam, 133, 151 stay resident, 218 wait for encoder position timed out, 90
top cover, 133, 151 sheet metal kit, 167 wait for pressure timed out, 84
resident mode condition, 53 shut-down, 57 wait for volume timed out, 83
results simple repair procedures, 114 warranty, 248
pressure test, 97 simple repairs, 110 responsibility of Agilent Technolo-
RS-232 snap fastener, 31, 34 gies, 250
cable kit to PC, 187 solvent cabinet, 42 services, 250
RS-232 connector, 30 solvent cabinet parts, 159 weight, 15, 16
RS-232 port, 212 solvent counter zero, 86 wide-ranging capability, 14, 30
RS-232C communication setting, 216 solvent filters wrong data from pressure sensor, 70
RS-232C connection table, 214 cleaning, 45
RS-232C interface, 214 solvent information, 42, 43, 256
run mode, 53 solvent inlet filter, 42
running the leak test, 102 sound emission, 255
running the pressure test, 95 specification
main power supply, 219
S performance, 246
safety specifications, 16
maintenance, 251 spectrum
safety information, 251 range, 229
on lithium batteries, 254 spurious index, 78
safety standards, 16 stack configuration, 21, 22, 23, 24, 25,
safety symbols, 252 26, 27
saftey front view, 22, 24, 26
operation, 251 rear view, 23, 25, 27
saftey lever, 29 startup error condition, 54
schematics status indicators, 52
preparative pump, 193 status light pipe, 133, 149, 172
seal wash, 46, 89 stay resident settings, 218
seal wash current low, 88 storage, 15
seal wash function, 42, 46 synchronization lost, 59
seal wash pump cassette, 114, 124
seal wash pump drive, 133, 147, 201 T
seal wash tubings, 32 temperature, 15
seal wear counter, 198 tests on control module, 244
seals, 42, 114, 125 time-out, 56
security lever, 28, 110 top cover, 133, 134, 151
serial number
entered on control module, 243 U
serial number change unpacking the pump, 17
control module, 243
setable flow range, 246 V
setting valves, 114, 115, 121
baudrate, 216 voltage range, 219
data bit, 217 voltage selector, 14, 30
forced cold-start, 217
parity, 217
262
s1
In This Book
This manual contains technical reference

information about the Agilent 1100 Series
preparative pump. The manual describes
the following:
• Installation,
• Optimizing performance,
• Diagnostics and troubleshooting,
• Repairing,
• Parts and materials,
• Introduction to the pump, theory of

operation,
• Screens of the local control module,
• Specifications and
• Legal safety and warranty information.

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Agilent 1100 Series

• Parts and materials,

• Introduction and theory of operation,

• Screens of local control,

• Legal, safety and warranty information.

1 Installing the Pump

Installing the Pump 14

Hints for Successful Use of the Preparative Pump 42

3 Troubleshooting and Test Functions

Status Indicators and Operation 50

Running the Test from the Control Module 102

4 Repairing the Pump

Simple Repairs 110

Simple Repair Procedures 114

Exchanging Internal Parts 132

5 Parts and Materials

Pump Housing and Main Assemblies 156

Foam Parts 168

Cable Overview 174

6 Introduction to the Preparative Pump

Introduction to the Preparative Pump 190

Preparative Pump Main Board (PPM) 201

7 Control Module Screens for the Preparative Pump

Major keys on the Agilent 1100 Control Module 222

Performance Specifications 246

Legal, Safety and Warranty Information

1 Installing the Pump

How to install the preparative pump.

Installing the Pump

A suitable site environment is important to ensure optimal performance of

WAR NI N G Shock hazard or damage of your instrumentation can result if the

Table 1 Physical Specifications - Preparative Pump

Type Specification Comments

Line frequency 50 – 60 Hz, ± 5 %

Operating Altitude Up to 2000 m (6500 ft)

Unpacking the Preparative Pump

Table 2 First Preparative Pump (G1361A) Delivery Checklist

Table 3 Gradient Preparative Pump (G1391A) Delivery Checklist

Description Part Number Quantity

Bottle head assembly G1361-60002 1

Description Part Number Quantity

Hex-key set 8710-0641 1

Description Part Number Quantity

Bottle head assembly G1361-60002 1

Optimizing the Stack Configuration

The following sample setups are shown on the following pages:

CAN Bus cable

CAN Bus cables

Agilent 220 micro LC/MS

CAN bus cable

Installing the preparative pump

Preparations Locate bench space

1 Place the preparative pump on the bench in a horizontal position.

Figure 7 Front View of the Preparative Pump

Figure 9 Preparative Pump Electrical Connections

Interface (BCD) board slot

Flow Connections for a single (isocratic)

Preparation Pump is installed in the HPLC system

Figure 10 Removing the Front Cover

2 Place the solvent cabinet on top of the preparative pump.

WAR NI N G Danger of Explosion! Do not use metal bottles without appropriate

7 Connect the Outlet Capillary (G1361-67302) to the electromagnetic purge

Figure 11 Flow connections to the preparative pump