Iron liquicolor Calculation of the Iron Concentration with Standard

If a different wavelength (620 nm - 640 nm) is to be used for measure-

Photometric Colorimetric Test for Iron with ment the standard provided with the kit has to be employed for the
calculation.
Lipid Clearing Factor (LCF)
A sample
CAB Method C = 100 x [µg/dl]
Package Sizes A [STD]
[REF] 10229 2 x 30 ml Complete Test Kit A sample
10230 2 x 100 ml Complete Test Kit C = 17.9 x [µmol/l]
A [STD]
[IVD]
Linearity
Method1
The test is linear up to an iron concentration of 500 µg/dl or 89.5 µmol/l.
Iron (III) reacts with chromazurol B (CAB) and cetyltrimethylammonium-
bromide (CTMA) to form a coloured ternary complex with an absorbance Reference Values3
maximum at 623 nm. The intensity of the colour produced is directly
Male: 59 - 148 µg/dl or 10.6 - 28.3 µmol/l
proportional to the concentration of iron in the sample.
Female: 37 - 145 µg/dl or 6.6 - 26 µmol/l
The test can also be used in combination with the TIBC kit ([REF] 10670)
to determine the total iron binding capacity. Quality Control
All control sera with iron values determined by this method can be
Contents
employed.
[RGT] 2 x 30 ml or 2 x 100 ml CAB Reagent
CAB 0.18 mmol/l We recommend to use our HUMATROL quality control sera based on
CTMA 2.2 mmol/l animal serum or our SERODOS based on human serum.
Guanidinium chloride 2.6 mol/l
Sodium acetate buffer (pH 4.7) 45 mmol/l Automation
Proposals to apply the reagents on analysers are available on request.
[STD] 5 ml Standard Each laboratory has to validate the application in its own responsibility.
Iron (ionised) 100 µg/dI
or 17.9 µmol/I Performance Characteristics
Typical performance data can be found in the Verification Report,
Reagent Preparation
accessible via:
[RGT] and [STD] are ready for use.
www.human.de/data/gb/vr/su-fe.pdf or
Reagent Stability www.human-de.com/data/gb/vr/su-fe.pdf
[RGT] is stable even after opening up to the stated expiry date when
stored at 2...25°C. Contamination of the reagents must be absolutely Notes
avoided. 1. This iron test is very sensitive.
To avoid contamination the glassware used must be iron free. We
Specimen
strongly recommend to use disposable laboratory materials when
Serum, heparinised plasma.
performing this test.
Do not use EDTA plasma, citrate plasma or haemolytic sera!
2. Make sure that the distilled water is absolutely iron free.
Note 3. Do not use turbid or haemolytic sera or plasma.
Lipemic specimens usually generate turbidity of the sample reagent 4. Bilirubin up to 15 mg/dl and copper up to 500 µg/dl do not interfere.
mixture which Ieads to falsely high results.
The IRON liquicolor test avoids these falsely high results by its built-in References
Lipid Clearing Factor (LCF). The LCF clears turbidity caused by lipemic 1. Garcic A. Clin. Chem. Acta 94, 115-119 (1979)
specimens during incubation. 2. Callahan J. H., Cook K. O., Anal. Chem. 54, 59-62 (1982)

Assay 3. Weippl G. et al., Blut 27, 261-270 (1973)

Wavelength:
Optical path:
623 nm, Hg 623 nm
1 cm
SU-FE INF 1022901 GB 07-2008-23 |
Temperature: 20...25°C
Measurement: against reagent blank (Rb).
Only one reagent blank per series is required.

Pipetting Scheme
Pipette into cuvettes: Rb Sample / [STD]
Sample / [STD] --- 50 µl
Distilled water 50 µl ---
[RGT] 1000 µl 1000 µl
Mix well, incubate for 15 minutes at 20...25°C. Measure the absorbance
of the sample ( A sample) and the standard ( A [STD]) against the reagent
blank within 60 minutes.

Calculation of the Iron Concentration with Factor

Wavelength Iron [µg/dl] Iron [µmol/I]
Hg 623 nm 830 x A sample 149 x A sample

Human Gesellschaft für Biochemica und Diagnostica mbH

Max-Planck-Ring 21 · 65205 Wiesbaden · Germany
Telefon +49 6122-9988-0 · Telefax +49 6122-9988-100 · e-Mail human@human.de