Diagnostic Reagents: Manual Methods

DIAGNOSTIC
REAGENTS
MANUAL METHODS
Version : MMAN-09 – April 2010
SEPPIM S.A.S.
ZONE INDUSTRIELLE
61500 SEES - FRANCE
: 02 33 81 21 00 : 02 33 28 77 51
REAGENTS
G Version
GAMMA GT SL........................................... MM-GASL-1
A Version GAMMA GT ............................................... MM-GAGT-1
ACID PHOSPHATASE .................................MM-PACI-1 GLUCOSE HK SL........................................ MM-GHSL-1
ALBUMIN .................................................. MM-ALBU-1 GLUCOSE PAP SL ...................................... MM-GPSL-2
GLUCOSE PAP............................................ MM-GLUP-1
ALP (DEA) SL.............................................. MM-PASL-1
ALP (DEA) ...................................................MM-PALC-1
ALT/GPT 4+1 SL .................................... MM-ALSL4/1-1 I
ALT/GPT ..................................................... MM-ALAT-2
IRON CHROMAZUROL ............................. MM-FECA-1
AMYLASE SL ............................................. MM-AMSL-3 IRON FERROZINE® ................................... MM-FEFR-2
AST/GOT 4+1 SL .................................... MM-ASSL4/1-1
AST/GOT ..................................................... MM-ASAT-2
L
LACTATE .................................................... MM-LACT-2
B New LDH-L SL......................................................MM-LLSL-1
BILIRUBIN TOTAL & DIRECT 4+1 .......... MM-BITD-2 LDH-P .......................................................... MM-LDHP-2
M
C MAGNESIUM CALMAGITE ................... MM-MAGN-1
CALCIUM ARSENAZO ............................. MM-CALA-1 MICROPROTEIN ........................................ MM-PRTP-2
CALCIUM OCPC ........................................ MM-CALO-1
Update CHLORIDE ................................................. MM-CHLO-2
CHOLESTEROL SL .....................................MM-CHSL-2 P
Update CHOLESTEROL ......................................... MM-CHOL-3 PHOSPHORUS ............................................ MM-PHOS-1
CHOLESTEROL HDL SL 2G .................... MM-HDLL-1
CHOLESTEROL LDL SL 2G ......................MM-LDLL-1
HDL CHOLESTEROL ................................ MM-HDLC-2
CHOLINESTERASE ....................................MM-CHES-2 T
TOTAL PROTEIN PLUS ............................. MM-PROB-1
CK MB SL ................................................... MM-CMSL-2
CK MB ........................................................ MM-CKMB-2 TRIGLYCERIDES MONO SL NEW ...... MM-TGMLN-2
TRIGLYCERIDES ........................................ MM-TRIG-2
CK NAC SL ..................................................MM-CKSL-2
CK NAC.......................................................MM-CKNA-2
COPPER........................................................ MM-CUIV-1 U
CREATININE JAFFE.................................. MM-CRCO-1 UREA UV SL ............................................... MM-URSL-2
CREATININE PAP SL ................................. MM-CRSL-3 UREA UV.................................................... MM-URUV-1
URIC ACID ................................................. MM-ACUR-2
URIC ACID MONO SL ..............................MM-AUML-2
Update URIC ACID SL ............................................ MM-AUSL-2
SEPPIM S.A.S.
ZONE INDUSTRIELLE
61500 SEES - FRANCE
: 02 33 81 21 00 : 02 33 28 77 51
ELITECH ELITECH ELITECH
ACID Ref. : PACI- 0030 18 x 3 mL
PHOSPHATASE
MANUAL METHOD
PROCEDURE
Wavelength : 405 nm
Temperature : 37°C
Cuvette : 1 cm light path
Read against reagent blank.
Total acid phosphatase Non-prostatic acid phosphatase

Working Reagent 1 1 mL 1 mL
Working Reagent 2 10 µL
Sample 100 µL 100 µL
Mix and after a 5 minute incubation measure the change of optical density per minute (∆OD/min.) during 3
minutes.
CALCULATION
Total ACP : Activity (U/L) = ∆OD/min. x 853

NP ACP : Activity (U/L) = ∆OD/min. x 860
Prostatic fraction (U/L) = Total ACP (U/L) - NP ACP (U/L).
LINEARITY
If ∆OD/min. exceeds 0.070 at 405 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L).
Multiply result by 10.
NOTE: For more details, see the Instructions for use
(07/2005)
MM-PACI-1
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ALBUMIN Ref.: ALBU - 0600

ALBU - 0700
2 x 125 mL
4 x 250 mL
MANUAL METHOD
PROCEDURE
Wavelength : 628 nm
Temperature : 37°C

BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 10 µL
Standard 10 µL
Sample 10 µL
Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 15 minutes.
CALCULATION
OD Sample
xn
OD Standard
n = standard concentration
(07/2005)
MM-ALBU-1

ALP (DEA) SL Réf. : PASL- 0400

PASL- 0420
2 x 62.5 mL
4 x 62.5 mL
PASL- 0500 5 x 125 mL
MANUAL METHOD
PROCEDURE
Wavelength : 405 nm (410)

Temperature : 37°C, 30°C, 25°C
Read against distilled water.
One-reagent procedure
Working reagent : 1 mL
Sample : 20 µL
Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3
minutes.
• Two-reagent procedure
R1 : 1 mL
Sample : 20 µL
Mix, wait 1 minute and add:
R2 : 250 µL
minutes.
CALCULATION
One reagent procedure Two reagents procedure
At 405 nm: Activity (U/L) = ∆OD/min. x 2 750 3424
LINEARITY
If ∆OD/min. exceeds 0.250 at 405 nm, repeat the test using serum diluted 1/10 with sodium chloride solution
(9 g/L). Multiply result by 10.
(07/2005)
MM-PASL-1

ALP (DEA) Réf. : PALC- 0030

PALC- 0200
20 x 3 mL
12 x 20 mL
MANUAL METHOD
PROCEDURE

Sample : 20 µL
minutes.
CALCULATION
At 405 nm: Activity (U/L) = ∆OD/min. x 2 750

LINEARITY
If ∆OD/min. exceeds 0.250 at 405 nm, repeat the test using serum diluted 1/10 with sodium chloride solution (9
g/L). Multiply result by 10.
(07/2005)
MM-PALC-1

ALT/GPT 4+1 SL Ref.: ALSL – 0410

ALSL – 0430
2 x 62.5 mL
4 x 62.5 mL
ALSL – 0510 5 x 125 mL
MANUAL METHOD
PROCEDURE
Wavelength : 340 nm
Temperature : 37°C
• One-reagent procedure
Sample : 100 µL
Mix and after a 1 minute incubation, measure the change of optical density per minute
(∆OD/min.) during 3 minutes.
R1 : 1 mL
Sample : 125 µL
R2 : 250 µL
CALCULATION
For the two procedures, at 340 nm: Activity (U/L) = ∆OD/min. x 1 746
LINEARITY
(07/2005)
MM-ALSL4/1-1

ALT/GPT Ref.: ALAT - 0200

ALAT - 0400
12 x 20 mL
9 x 50 mL
MANUAL METHOD
PROCEDURE
Wavelength : 340 nm (334 – 365)

Temperature : 30°C, 37°C
Sample : 100 µL
minutes.
CALCULATION

At 365 nm: Activity (U/L) = ∆OD/min. x 3235
LINEARITY
(03/2008)
MM-ALAT-2

AMYLASE SL Réf. : AMSL - 0390

AMSL - 0400
1 x 50 mL
6 x 50 mL
MANUAL METHOD
PROCEDURE
Wavelength : 405 nm
37 °C 30 °C
Reagent 1 mL 1 mL
Sample 15 µL 25 µL
minutes.
CALCULATION
37 °C 30 °C
Take dilution factor into account for the calculation of the activity in urine.
LINEARITY
Up to 900 U/L
If ∆OD/min. exceeds 0.190 at 405 nm, repeat test using sample diluted 1/10 with sodium chloride solution
(11/2006)
MM-AMSL-3

AST/GOT 4+1 SL Réf. : ASSL- 0410

ASSL- 0430
2 x 62.5 mL
4 x 62.5 mL
ASSL- 0510 5 x 125 mL
MANUAL METHOD
PROCEDURE
Wavelength : 340 nm
Temperature : 37°C
Sample : 100 µL
R1 : 1 mL
Sample : 125 µL
R2 : 250 µL
CALCULATION
For the two procedures, at 340 nm: Activity (U/L) = ∆OD/min. x 1 746
LINEARITY
(07/2005)
MM-ASSL4/1-1

AST/GOT Ref.: ASAT - 0200

ASAT - 0400
12 x 20 mL
9 x 50 mL
MANUAL METHOD
PROCEDURE
Wavelength : 340 nm (334 – 365)

Sample : 100 µL
minutes.
CALCULATION

LINEARITY
(07/2008)
MM-ASAT-2

BILIRUBIN Ref.: BITD – 0600 Total

Direct
125 mL
125 mL
TOTAL & DIRECT 4+1 BITO - 0600
BIDI - 0600
Total 2 x 125 mL
Direct 2 x 125 mL
MANUAL METHOD
PROCEDURE for TOTAL BILIRUBIN

Wavelength : 550 nm Temperature : 37°C Cuvette : 1 cm light path

CALIBRATION TEST
R1 BILIRUBIN TOTAL 4+1 800 µL 800 µL
Calibrator 100 µL
Sample 100 µL
Mix and read the optical density (OD) after 5 minutes of incubation at 37°C
OD1 OD2
Then add:
R2 BILIRUBIN TOTAL & DIRECT 4+1 200 µL 200 µL
OD3 OD4
PROCEDURE for DIRECT BILIRUBIN

Wavelength : 550 nm Temperature : 37°C Cuvette : 1 cm light path

CALIBRATION TEST
R1 BILIRUBIN DIRECT 4+1 800 µL 800 µL
Calibrator 100 µL
Sample 100 µL
OD1 OD2
Then add:
R2 BILIRUBIN TOTAL & DIRECT 4+1 200 µL 200 µL
OD3 OD4
CALCULATION FOR BOTH PROCEDURES (Total and Direct Bilirubin)

With Factor: (OD4 - OD2) x F With calibrator: OD4 - OD2 x n
OD3 - OD1
mg/dL: F = 14.0
mg/L: F = 140 n = Calibrator concentration
µmol/L: F = 240

(08/2009)
MM-BITD-2

CALCIUM Ref.: CALA - 0600 2 x 125 mL
ARSENAZO
MANUAL METHOD
PROCEDURE

Temperature : 37°C

Standard 10 µL
Sample 10 µL
The final colour is stable for at least 1 hour.
CALCULATION
OD Sample
xn
OD Standard
Take dilution factor into account for the calculation of the concentration in urine.
(07/2005)
MM-CALA-1

CALCIUM OCPC Ref.: CALO - 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE
Wavelength : 570 nm
Temperature : 37°C

Working Reagent 1 mL 1 mL 1 mL
Standard 10 µL
Sample 10 µL
CALCULATION
OD Sample
xn
OD Standard
(07/2005)
MM-CALO-1

CHLORIDE Réf. : CHLO – 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE
Wavelength : 480 nm

Standard 10 µL
Sample 10 µL
Information for urine sample: The urine sample must be diluted ½ with distilled water before analysis.
CALCULATION
Serum/Plasma: Urine:
OD Sample OD Sample
n n2
OD Standard OD Standard
n= Standard concentration
(09/2009)
MM-CHLO-2

CHOLESTEROL SL Réf. : CHSL- 0490

CHSL- 0500
1
6
x
x
100
100
mL
mL
CHSL- 0700 4 x 250 mL
CHSL- 0507 6 x 100 mL*
CHSL- 0707 4 x 250 mL*
MANUAL METHOD
PROCEDURE
Wavelength : 500 nm (492-550)

Temperature : 37°C

Standard 10 µL
Sample 10 µL
CALCULATION
OD Sample x n
OD Standard
(07/2007)
* these kits contain a standard MM-CHSL-2
SEPPIM S.A.S. ZONE INDUSTRIELLE - 61500 SEES - FRANCE

CHOLESTEROL Ref.: CHOL - 0220

CHOL - 0420
12 x 20 mL
9 x 50 mL
MANUAL METHOD
PROCEDURE
Wavelength : 500 nm (492 – 550)

Temperature : 37°C

Standard 10 µL
Sample 10 µL
CALCULATION
OD Sample x n
OD Standard
(03/2010)
MM-CHOL-3

CHOLESTEROL Ref.: HDLL – 0380

HDLL – 0390
1 x 80 mL
3 x 80 mL
HDL SL 2G
MANUAL METHOD
PROCEDURE
Wavelength : 600 nm
Temperature : 37°C

R1 900 µL 900 µL 900 µL
Distilled water 9 µL
Standard 9 µL
Sample 9 µL
Mix and read absorbance (A1) after 5 minutes of incubation at 37°C.
Then add:
R2 300 µL 300 µL 300 µL
Mix and read absorbance (A2) after 5 minutes of incubation at 37°C
CALCULATION
( A2 − A1 × Fdil ) Sample
×n n = Calibrator concentration
( A2 − A1 × Fdil )Calibrator
Fdil = Dilution factor = (R1 volume + sample volume)/(R1 volume+R2 volume + sample volume)
(04/2009)
MM-HDLL-1

CHOLESTEROL Ref.: LDLL – 0380 1 x 80 mL
LDL SL 2G
MANUAL METHOD
PROCEDURE
Wavelength : 550 nm
Temperature : 37°C

R1 900 µL 900 µL 900 µL
Distilled water 9 µL
Standard 9 µL
Sample 9 µL
Mix and read absorbance (A1) after 5 minutes of incubation at 37°C.
Then add:
R2 300 µL 300 µL 300 µL
Mix and read absorbance (A2) after 5 minutes of incubation at 37°C
CALCULATION
( A2 − A1 × Fdil ) Sample
×n n = Calibrator concentration
( A2 − A1 × Fdil )Calibrator
Fdil = Dilution factor = (R1 volume + sample volume)/(R1 volume+R2 volume + sample volume)
(04/2009)
MM-LDLL-1

HDL Ref.: HDLC - 0060 3 x 10 mL
CHOLESTEROL
MANUAL METHOD
PROCEDURE
1) Sample preparation:
Add to 500 µL of sample, 50 µL of precipitating reagent. Mix, wait for 10 minutes and centrifuge at 5000 rpm
for 15 minutes. The supernatant is collected for HDL determination.
2) HDL determination:

Temperature : 37°C

Standard 50 mg/dL 50 µL
Supernatant 50 µL
CALCULATION
OD Sample x n x 1.1
OD Standard
n = Standard concentration
1.1 = Sample dilution factor
(09/2009)
MM-HDLC-2

CHOLINESTERASE Ref.: CHES - 0053 16 x 3 mL
MANUAL METHOD
PROCEDURE
Wavelength : 405 nm (400 –440)

Working reagent 1 : 3 mL
Sample : 20 µL
Working reagent 2 : 100 µL
Mix and measure the change of optical density every 30 seconds (∆OD/30 s.) during 90 seconds.
CALCULATION
Activity (U/L) = ∆OD/30 s. x 23 460
LINEARITY
If ∆OD/30 sec. exceeds 0.200 at 405 nm, repeat test using serum diluted 1/5 with sodium chloride solution
(03/2008)
MM-CHES-2

CK MB SL Réf. : CMSL- 0410

CMSL- 0430
2 x 62.5 mL
4 x 62.5 mL
MANUAL METHOD
PROCEDURE
Wavelength : 340 nm
Sample : 40 µL
minutes.
)• Two-reagent procedure
R1 : 1 mL
R2 : 250 µL
Mix and wait 30 seconds
Sample : 50 µL
minutes.
CALCULATION
For the two procedures, at 340 nm: Activity (U/L) = ∆OD/min. x 8254
LINEARITY
Inhibition : up to 2 000 U/L of CK-MM at 37° C.

If ∆OD/min. exceeds 0.075 dilute the sample (one part with nine parts saline solution), repeat the test and
multiply the result by 10.
Linear up at least 600 U/L.
(11/2008)
): Modification from the previous version MM-CMSL-2

CK MB Réf. : CKMB- 0030 20 x 3 mL
MANUAL METHOD
PROCEDURE
Wavelength : 340 nm
Sample : 50 µL
minutes.
CALCULATION
a) Total CK activity :
Determine total CK activity in serum using the CK-NAC reagent.
b) CK-B activity :
CK-B (U/L) = ∆OD/min x 3 333
c) CK-MB activity :
CK-MB (U/L) = CK-B (U/L) x 2
d) Percentage of CK-MB activity in sample :

% CK-MB = CK-MB x 100
Total-CK
LINEARITY
Inhibition : up to 2000 U/L of CK-MM at 37° C.

If the assay of the total CK exceeds 1200 U/L at 37°C, dilute the sample appropriately with physiological saline
before assay of CK-MB.
Multiply the result by the dilution factor to obtain the correct value of the isoenzyme.
(05/2006)
MM-CKMB-2

CK NAC SL Réf. : CKSL- 0410

CKSL- 0430
2 x 62.5 mL
4 x 62.5 mL
MANUAL METHOD
PROCEDURE
Wavelength : 340 nm
Sample : 40 µL
minutes.
)• Two-reagent procedure
R1 : 1 mL
R2 : 250 µL
Mix and wait 30 seconds
Sample : 50 µL
minutes.
CALCULATION
For the two procedures, at 340 nm: Activity (U/L) = ∆OD/min. x 4127
LINEARITY
(11/2008)
): Modification from the previous version MM-CKSL-2

CK NAC Réf. : CKNA- 0030 20 x 3 mL
MANUAL METHOD
PROCEDURE

Sample : 20 µL
minutes.
CALCULATION
At 340 nm : Activity (U/L) = ∆OD/min. x 8 095

LINEARITY
(03/2008)
MM-CKNA-2

COPPER Réf. : CUIV- 0050 5 x 10 mL
MANUAL METHOD
PROCEDURE

Temperature : 37°C

Working Reagent 2 1 mL 1 mL 1 mL
Standard 50 µL
Sample 50 µL

Working Reagent 1 1 mL 1 mL 1 mL
Standard 50 µL
Sample 50 µL
Mix and read the optical density (OD1).
Reagent 2 50 µL 50 µL 50 µL
Mix and read the optical density (OD2) after a 5 minute incubation.
CALCULATION
• One-reagent procedure : • Two-reagent procedure :
OD Sample xn OD2 - OD1 Sample xn

OD Standard OD2 - OD1 Standard
(07/2005)
MM-CUIV-1

CREATININE Ref.: CRCO - 0600

CRCO - 0700
2 x 125 mL
4 x 250 mL
JAFFE
MANUAL METHOD
PROCEDURE
Wavelength : 492 nm (480 –520)

Temperature : 37°C
Sample : 100 µL
Mix and read the optical density (OD1) 10 seconds after the sample or standard addition. Exactly 2 minutes
after the first reading, take second reading (OD2).
CALCULATION
∆OD Sample xn
∆OD Standard
Take dilution factor into account for calculation of concentration in urine.
(07/2005)
MM-CRCO-1

CREATININE Ref.: CRSL - 0630 2 x 133 mL
PAP SL
MANUAL METHOD
PROCEDURE
Wavelength : 550 nm
Temperature : 37°C

R1 1.5 mL 1.5 mL 1.5 mL
Standard 35 µL
Sample 35 µL
Mix, and wait 5 minutes at 37°C. Then, add:
R2 0.5 mL 0.5 mL 0.5 mL
Mix and read the change of optical density (∆OD) between 1 and 4 minutes..
CALCULATION
∆OD Sample
xn
∆OD Standard
(03/2008)
MM-CRSL-3

GAMMA GT SL Ref.: GASL - 0400

GASL - 0420
2 x 62.5 mL
4 x 62.5 mL
GASL - 0500 5 x 125 mL
MANUAL METHOD
PROCEDURE
Wavelength : 405 nm
Sample : 100 µL
R1 : 1 mL
Sample : 100 µL
R2 : 250 µL
CALCULATION
One reagent procedure Two reagents procedure
LINEARITY
(07/2005)
MM-GASL-1

GAMMA GT Ref.: GAGT – 0030

GAGT – 0200
20 x 3 mL
12 x 20 mL
MANUAL METHOD
PROCEDURE
Wavelength : 405 nm
Sample : 100 µL
minutes.
CALCULATION
LINEARITY
(07/2005)
MM-GAGT-1

GLUCOSE HK SL Ref.: GHSL - 0600 5 x 125 mL
MANUAL METHOD
PROCEDURE

Temperature : 37°C

Standard 10 µL
Sample 10 µL
• Two-reagents procedure

R1 1 mL 1 mL 1 mL
Standard 10 µL
Sample 10 µL
R2 250 µL 250 µL 250 µL
CALCULATION
OD Sample x n
OD Standard
(07/2005)
MM-GHSL-1

GLUCOSE PAP SL Ref.: GPSL - 0490

GPSL - 0500
1 x 100 mL
6 x 100 mL
GPSL - 0700 4 x 250 mL
GPSL - 0507 6 x 100 mL*
GPSL - 0707 4 x 250 mL*
MANUAL METHOD
PROCEDURE

Temperature : 37°C

Standard 10 µL
Sample 10 µL
Mix and measure the optical density (OD) after a 10 minute incubation.
The reading can be done during 30 minutes.
CALCULATION
OD Sample x n
OD Standard
(07/2007)
* these kits contain a standard MM-GPSL-2

GLUCOSE PAP Ref.: GLUP - 0700

GLUP - 0800
4 x 250 mL
5 x 500 mL
MANUAL METHOD
PROCEDURE

Temperature : 37°C

Standard 10 µL
Sample 10 µL
CALCULATION
OD Sample x n
OD Standard
(07/2005)
MM-GLUP-1

IRON Ref.: FECA - 0600 2 x 125 mL
CHROMAZUROL
MANUAL METHOD
PROCEDURE
Wavelength : 637 nm
Temperature : 37°C

Standard 50 µL
Sample 50 µL
CALCULATION
OD Sample x n
OD Standard
(07/2005)
MM-FECA-1

IRON FERROZINE Ref.: FEFR – 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE

Temperature : 37°C

Standard 200 µL
Sample 200 µL
Mix and read the optical density (OD).
- OD1 OD2
Then add:
R2 50 µL 50 µL 50 µL
Mix and read the optical density (OD) within one hour.
- OD3 OD4
CALCULATION
OD4 - OD2
xn
OD3 - OD1
(09/2009)
MM-FEFR-2

LACTATE Réf. : LACT- 0100 10 x 10 mL
MANUAL METHOD
PROCEDURE

Temperature : 37°C

Standard 10 µL
Sample 10 µL
CALCULATION
OD Sample x n
OD Standard
(05/2006)
MM-LACT-2

LDH-L SL Ref.: LLSL - 0400

LLSL - 0420
2 x 62.5 mL
4 x 62.5 mL
MANUAL METHOD
PROCEDURE
Wavelength : 340 nm
Temperature : 37°C
BLANK SAMPLE
R1 1 mL 1 mL
Sample 35 µL
R2 250 µL 250 µL
Mix and after a 100 second incubation, measure the change of optical density per minute
CALCULATION
(10/2007)
MM-LLSL-1

LDH-P Ref. : LDHP - 0030 20 x 3 mL
MANUAL METHOD
PROCEDURE

Sample : 20 µL
minutes.
CALCULATION

LINEARITY
If ∆OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/5 or 1/10 with sodium chloride solution
(9 g/L). Multiply result by 5 or 10.
(03/2008)
MM-LDHP-2

MAGNESIUM Ref.: MAGN - 0600 2 x 125 mL
CALMAGITE
MANUAL METHOD
PROCEDURE

Temperature : 37°C

Standard 10 µL
Sample 10 µL
CALCULATION
OD Sample x n
OD Standard
Take dilution factor into account for the calculation of magnesium concentration in urine.
(07/2005)
MM-MAGN-1

MICROPROTEIN Ref.: PRTP - 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE
Wavelength : 598 nm
Temperature : 37°C

Standard 20 µL
Sample 20 µL
) The final colour is stable for 10 minutes.
CALCULATION
OD Sample x n
OD Standard
(11/2008)
): Modification from the previous version MM-PRTP-2

PHOSPHORUS Ref.: PHOS - 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE
Wavelength : 340 nm
Temperature : 37°C

Standard 10 µL
Sample 10 µL
CALCULATION
OD Sample x n
OD Standard
Take dilution factor into account for the calculation of phosphorus concentration in urine.
(07/2005)
MM-PHOS-1

TOTAL PROTEIN PLUS Réf.: PROB - 0600

PROB - 0700
2 x 125 mL
4 x 250 mL
MANUAL METHOD
PROCEDURE
Wavelength : 546 nm
Temperature : 37°C

Standard 10 µL
Sample 10 µL
Mix and read the optical density (OD) after 10 minutes incubation.
The final colour is stable for 20 minutes.
CALCULATION
OD Sample
 n
OD Standard
(08/2009)
MM-PROB-1

TRIGLYCERIDES Réf. : TGML-0425

TGML-0515
6 x 50 mL
6 x 100 mL
TGML-0700 4 x 250 mL
MONO SL NEW TGML-0427
TGML-0517
6 x 50 mL*
6 x 100 mL*
TGML-0707 4 x 250 mL*
MANUAL METHOD
PROCEDURE
Wavelength : 500 nm
Temperature : 37°C

Standard 10 µL
Sample 10 µL
CALCULATION
OD Sample x n
OD Standard
(07/2007)
* these kits contain a standard MM-TGMLN-2

TRIGLYCERIDES Ref.: TRIG - 0200

TRIG - 0400
12 x 20 mL
9 x 50 mL
MANUAL METHOD
PROCEDURE
Wavelength : 546 nm (520 – 570)

Temperature : 37°C

Standard 10 µL
Sample 10 µL
CALCULATION
OD Sample x n
OD Standard
(07/2007)
MM-TRIG-2

UREA UV SL Réf. : URSL- 0400

URSL- 0420
2
4
x
x
62.5
62.5
mL
mL
URSL- 0500 5 x 125 mL
URSL- 0407 2 x 62.5 mL*
URSL- 0427 4 x 62.5 mL*
URSL- 0507 5 x 125 mL*
MANUAL METHOD
PROCEDURE
Wavelength : 340 nm
Temperature : 37°C
Sample : 10 µL
Mix and read the variation of optical density (∆OD) between 30 seconds and 90 seconds.
R1 : 1 mL
Sample : 10 µL
R2 : 250 µL
CALCULATION
∆OD Sample xn
∆OD Standard
(07/2007)
* these kits contain a standard MM-URSL-2

UREA UV Réf. : URUV-0400

URUV-0500
9 x 50 mL
6 x 100 mL
MANUAL METHOD
PROCEDURE
Wavelength : 340 nm

Standard 10 µL
Sample 10 µL
CALCULATION
∆OD Sample x n
∆OD Standard
Take the dilution factor into account for the calculation of urea concentration in urine.
(07/2005)
MM-URUV-1

URIC ACID Réf. : ACUR - 0200

ACUR - 0400
12 x 20 mL
9 x 50 mL
MANUAL METHOD
PROCEDURE

Temperature : 37°C

Standard 25 µL
Sample 25 µL
CALCULATION
OD Sample x n
OD Standard
Take the dilution factor into account for the calculation of uric acid concentration in urine.
(09/2009)
MM-ACUR-2
SEPPIM S.A..S- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

URIC ACID Réf. : AUML - 0420

AUML - 0500
6 x 50 mL
6 x 100 mL
AUML - 0700 4 x 250 mL
MONO SL AUML - 0427
AUML - 0507
6 x 50 mL*
6 x 100 mL*
AUML - 0707 4 x 250 mL*
MANUAL METHOD
PROCEDURE
Wavelength : 555 nm
Temperature : 37°C

Standard 25 µL
Sample 25 µL
CALCULATION
OD Sample x n
OD Standard
(07/2007)
* these kits contain a standard MM-AUML-2

URIC ACID SL Réf. : AUSL – 0400

AUSL – 0600
2 x 62.5 mL
5 x 125 mL
MANUAL METHOD
PROCEDURE
Wavelength : 555 nm
Temperature : 37°C

Standard 25 µL
Sample 25 µL

R1 1 mL 1 mL 1 mL
Standard 25 µL
Sample 25 µL
R2 250 µL 250 µL 250 µL
CALCULATION
OD Sample x n
OD Standard
(03/2010)
MM-AUSL-2

Diagnostic Reagents: Manual Methods

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Diagnostic Reagents: Manual Methods

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DIAGNOSTIC

Version : MMAN-09 – April 2010

ACID Ref. : PACI- 0030 18 x 3 mL

Read against reagent blank.

Total acid phosphatase Non-prostatic acid phosphatase

Total ACP : Activity (U/L) = ∆OD/min. x 853

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ALBUMIN Ref.: ALBU - 0600

Read against reagent blank.

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ALP (DEA) SL Réf. : PASL- 0400

Wavelength : 405 nm (410)

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ALP (DEA) Réf. : PALC- 0030

Wavelength : 405 nm (410)

Read against distilled water.

At 405 nm: Activity (U/L) = ∆OD/min. x 2 750

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ALT/GPT 4+1 SL Ref.: ALSL – 0410

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ALT/GPT Ref.: ALAT - 0200

Wavelength : 340 nm (334 – 365)

Read against distilled water.

At 340 nm: Activity (U/L) = ∆OD/min. x 1 746

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

AMYLASE SL Réf. : AMSL - 0390

Read against distilled water.

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

AST/GOT 4+1 SL Réf. : ASSL- 0410

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

AST/GOT Ref.: ASAT - 0200

Wavelength : 340 nm (334 – 365)

Read against distilled water.

At 340 nm: Activity (U/L) = ∆OD/min. x 1 746

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

BILIRUBIN Ref.: BITD – 0600 Total

PROCEDURE for TOTAL BILIRUBIN

Read against distilled water.

PROCEDURE for DIRECT BILIRUBIN

Read against distilled water.

CALCULATION FOR BOTH PROCEDURES (Total and Direct Bilirubin)

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

CALCIUM Ref.: CALA - 0600 2 x 125 mL

Wavelength : 650 nm (600)

Read against distilled water.

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

CALCIUM OCPC Ref.: CALO - 0600 2 x 125 mL

Read against distilled water.

BLANK STANDARD SAMPLE

NOTE: For more details, see the Instructions for use

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

CHLORIDE Réf. : CHLO – 0600 2 x 125 mL