ASPARTATE

COD 21531 8 x 60 mL + 8 x 15 mL AMINOTRANSFERASE

(AST/GOT)
STORE AT 2-8ºC
Reagents for measurement of AST/GOT concentration ASPARTATE AMINOTRANSFERASE (AST/GOT)
Only for in vitro use in the clinical laboratory IFCC

PRINCIPLE OF THE METHOD QUALITY CONTROL

Aspartate aminotransferase (AST or GOT) catalyzes the transfer of the amino group from It is recommended to use the Biochemistry Control Serum level I (cod. 18005, 18009 and
aspartate to 2-oxoglutarate, forming oxalacetate and glutamate. The catalytic concentration is 18042) and II (cod. 18007, 18010 and 18043) to verify the performance of the measurement
determined from the rate of decrease of NADH, measured at 340 nm, by means of the malate procedure.
dehydrogenase (MDH) coupled reaction1,2,3. Each laboratory should establish its own internal Quality Control scheme and procedures for
AST corrective action if controls do not recover within the acceptable tolerances.
Aspartate + 2 - Oxoglutarate Oxalacetate + Glutamate
METROLOGICAL CHARACTERISTICS
MDH
Oxalacetate + NADH + H+ Malate + NAD+ − Detection limit: 1.1 U/L = 0.018 µkat/L

COMPOSITION − Linearity limit: 500 U/L = 8.33 µkat/L. For higher values dilute sample 1/ 10 with distilled
water and repeat measurement.
A. Reagent: 8 x 60 mL. Tris 121 mmol/L, L-aspartate 362 mmol/L, malate dehydrogenase >
− Repeatibility (within run):
460 U/L, lactate dehydrogenase > 660 U/L, pH 7.8.
Irritant (Xi): R36/38: Irritating to eyes and skin. S26: In case of contact with eyes, rinse Mean Concentration CV n
immediately with plenty of water and seek medical advice. S37/39: Wear suitable gloves and 38 U/L = 0.63 µkat/L 1.4 % 20
eye/face protection. 119 U/L = 1.98 µkat/L 1.5 % 20
B. Reagent: 8 x 15 mL. NADH 1.9 mmol/L, 2-oxoglutarate 75 mmol/L, sodium hydroxide 148 − Reproducibility (run to run):
mmol/L, sodium azide 9.5 g/L.
Mean Concentration CV n
Harmful (Xn): R22: Harmful if swallowed. R31: Contact with acids liberates toxic gas.
S28.1: After contact with skin, wash immediately with plenty of water. S45: In case of 38 U/L = 0.63 µkat/L 5.9 % 25
accident or if you feel unwell, seek medical advice immediately. 119 U/L = 1.98 µkat/L 3.8 % 25

STORAGE − Trueness: Results obtained with this reagent did not show systematic differences when
compared with reference reagents. Details of the comparison experiments are available on
Store at 2-8ºC. request.
Reagents are stable until the expiry date shown on the label when stored tightly closed and if − Interferences: Bilirubin (20 mg/dL) does not interfere. Lipemia (triglycerides 2 g/L) and
contaminations are prevented during their use. hemolysis may affect the results. Other drugs and substances may interfere5.
Indications of deterioration: These metrological characteristics have been obtained using an analyzer. Results may vary if a
− Reagents: Presence of particulate material, turbidity, absorbance of the blank lower than different instrument or a manual procedure are used.
1.400 at 340 nm (1 cm cuvette).
DIAGNOSTIC CHARACTERISTICS
SAMPLES The aminotransferases catalyze the formation of glutamic acid from 2-oxoglutarate by transfer of
Serum collected by standard procedures. amino groups. AST is found in highest concentration in the liver and heart muscle but it is also
abundant in skeletal muscle, kidney and pancreas.
Aspartate aminotransferase in serum is stable for 7 days at 2-8ºC.
The serum concentration of AST is elevated in hepatitis and other forms of hepatic disease
REFERENCE VALUES associated with necrosis: infectious mononucleosis, cholestasis, cirrhosis, metastasic carcinoma
of the liver, delirium tremens, and after administration of various drugs4,6.
Reaction temperature 37ºC 30ºC Serum AST concentration is also elevated after myocardial infarction, in sketetal muscle disease
(as progressive muscular distrophy), in acute pancreatitis or hemolytic disease and other4,6.
Without pyr-P, up to4 40 U/L = 0.67 µkat/L 25 U/L = 0.42 µkat/L
With pyr-P, up to1,2 50 U/L = 0.83 µkat/L 30 U/L = 0.50 µkat/L Clinical diagnosis should not be made on the findings of a single test result, but should integrate
both clinical and laboratory data.
Concentrations in newborns and infant are higher than in adults. Values in men are slightly
higher than in women. BIBLIOGRAPHY
These ranges are given for orientation only; each laboratory should establish its own reference 1. Sociedad Española de Química Clínica, Comité Científico, Comisión de Enzimas. Método
ranges. recomendado para la determinación en rutina de la concentración catalítica de la aspartato
aminotransferasa en suero sanguíneo humano. Quim Clin 1987; 6: 235-239.
CALIBRATION 2. Approved recommendations (1985) on IFCC Methods for the Measurement of Catalytic
It is recommended to use a serum based calibrator (Biochemistry Calibrator, cod. 18011 and Concentration of Enzymes. Part 2: IFCC Method for Aspartate Aminotransferase (EC
18044). 2.6.1.1). J Clin Chem Clin Biochem 1986; 24:497-510.
3. Gella FJ, Olivella T, Cruz Pastor M, Arenas J, Moreno R, Durban R and Gómez JA. A simple
ASSAY PARAMETERS procedure for routine determination of aspartate aminotransferase and alanine
These reagents may be used in several automatic analysers. Specific instructions for application aminotransferase with pyridoxal phosphate. Clin Chim Acta 1985; 153: 241-247.
in many of them are available on request. 4. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 4th ed. Burtis CA, Ashwood
− Wavelength: 340 nm ER, Bruns DE. WB Saunders Co, 2005.
− Temperature: 37ºC 5. Young DS. Effects of drugs on clinical laboratory tests, 5th ed. AACC Press, 2000.
− Analysis mode: kinetic 6. Friedman and Young. Effects of disease on clinical laboratory tests, 4th ed. AACC Press,
Blank Sample / Standard 2001.

Sample / Standard - 25 µL
Distilled water 25 µL -
Reagent A 240 µL 240 µL
Mix and incubate 3 - 5 minutes. Then add:

Reagent B 60 µL 60 µL
Mix, incubate 1 minute and read initial absorbance and at intervals thereafter for 3 minutes.

M21531i-02 BioSystems S.A. Costa Brava, 30. 08030 Barcelona (Spain) 01/2014
Quality System certified according to
EN ISO 13485 and EN ISO 9001 standards
 ASPARTATE
COD 21531 8 x 60 mL + 8 x 15 mL AMINOTRANSFERASE
(AST/GOT)
STORE AT 2-8ºC
Reagents for measurement of AST/GOT concentration ASPARTATE AMINOTRANSFERASE (AST/GOT)
Only for in vitro use in the clinical laboratory IFCC

M21531i-02 BioSystems S.A. Costa Brava, 30. 08030 Barcelona (Spain) 01/2014
Quality System certified according to
EN ISO 13485 and EN ISO 9001 standards