1 Insert data for x and y axis in a table

Concentration
A250 (y)
ug/ml (x)
0 0.002
Do not include
10 0.168 data for sample as
20 0.329 you need to
30 0.508 calculate it later.
40 0.66
50 0.846

2 Highlight all the data in the table > Insert >

Charts (Scatter)

Chart Title
1
0.8
0.6
0.4
0.2
0
0 10 20 30 40 50 60
3 Change Chart Title by clicking on the "Chart Title" and type the actual title.

Calibration Curve
Absorbance (250 nm) 1

0.8

0.6

0.4

0.2

0
0 10 20 30 40 50 60
Concentration, ug/ml

4 Insert Axis Title

Click Chart > Design > Add Chart Element > Axis Title > Primary Horizontal
& Primary Vertical

Calibration Curve
Absorbance (250 nm)

0.5

0
0 10 20 30 40 50 60
Concentration, ug/ml
5 Insert Trendline, Equation and R2
Click chart > Click + on the right > Click Trendline > Click arrow and More
Options > Scroll down and Click Equation and R2