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CONTENTS
• PHOTOSYNTHESIS
• BIOGAS
• FEED OF BIO-DIGESTER
• DESIGN OF BIO-DIGESTER
INTRODUCTION
• The longer the wavelength the lower the energy, thus the
lower the energy.
Sunlight
Sunlight is actually white light made of all wavelength
colors
Sunlight is visible light
Different colors=different wavelengths of light
380 nm 750 nm
Chlorophyll (the photosynthetic pigment)
Both photosystems are identical chlorophyll a molecules, except that they interact
with different proteins of the thylakoids
Excited electrons that have absorbed photons of light pass from molecule to
molecule until they reach the chlorophyll at the center of the photosystem
The photosystem (the chlorophyll) will then pass the excited electrons to a chain of
electron carriers
C. Oxygen production
Photosystem II absorbs light
Its electrons become excited
Photosystem II donates its electrons to an electron transport chain and the
flow of electrons will generate an ATP molecule
Photosystem II has been oxidized (LEO)
To get the electrons back (that were donated) an enzyme in the center of
photosystem II breaks a water molecule (photolysis)
The water is split into hydrogen ions, oxygen and electrons
Electrons are donated to PS II (GER)
Oxygen and hydrogen ions are byproducts
Oxygen is released to the atmosphere
The production of oxygen in photosynthesis is done by photolysis and requires
sunlight.
D. ATP Production
**IMPORTANT.**
1. Chemiosmosis involves the pumping of H+ ions through the
membrane.
2. The protons go from the stroma to the thylakoid space.
3. This creates a proton gradient.
4. The protons later flow through ATP synthase (back to the stroma) and
their energy is captured in order to join a phosphate with ADP
5. This produces ATP.
Light-independent reactions
Calvin cycle-
1. takes place in the stroma
2. begins with a 5 carbon sugar called ribulose biphosphate
3. Ribulose biphosphate = RuBP
4. ATP and NADPH from the light dependent reactions drive the Calvin cycle
5. ATP provides the energy
6. NADPH provides reducing power
Carbon is:
-absorbed as carbon dioxide
-released as sugar
B. ATP=energy for reactions
NADPH=reducing agent
C. Net sugar production per turn (3 carbon dioxide and 3 RuBP) is 1 G3P.
Limiting Factors in Photosynthesis
For photosynthesis to occur the following criteria must be met:
-Suitable temperature
-Presence of: chlorophyll
light
carbon dioxide
water
Changes to one limiting factor will change the rate of photosynthesis
Limiting factors are those that are near their minimum or maximum level
Limiting factors determine the rate-limiting step
For example: If light intensity is the limiting factor, the light dependent reaction will
limit the rate of photosynthesis.
The limiting-step will be the reduction reaction in the Calvin cycle (when the
products of the light dependent stage are needed)
E. Light as a limiting factor
1. At low light NADPH and ATP are not produced (b/c they are light-
dependent products)
2. If NADPH and ATP are not produced the Calvin cycle will stop at the
reduction and phosphorylation reactions
Rate of photosynthesis
Light intensity
F. Carbon dioxide as a limiting factor
1. If there is little or no carbon dioxide the Calvin cycle is limited at carbon
fixation
Rate of photosynthesis
1. At low temperature the enzymes that catalyze the reactions work slowly
2. At high temperature rubisco is ineffective (it is denatured)
3. Carbon fixation becomes the rate-limiting step
Temperature
BIOGAS
Introduction
The main source of raw material for production of biogas is Plant and
Animal biomass .
Biogas is primarily methane (CH4) and carbon dioxide (CO2) and may
have small amounts of hydrogen sulfide (H2S), moisture and siloxanes.
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TYPICAL GAS PRODUCTION FROM DIFFERENT FEEDSTOCK
Sl. Typical Gas Typical Methane
Feedstock
No. Yield (liters/kg) Content (%)
1. Paper Waste 480 53
2. Bagasse 330 57
3. Spent Tea Waste 235 57
4. Food Waste 160 62
5. Bamboo Pulp 145 54
6. Dry Leaves 118 60
7. Green leaves & 100 65
twigs
8. Fruit waste 91 50
9. Bamboo dust 53 72
10. Distillery effluent 31 75
11. Black liquor 22 69
(Paper Mill)
12. Animal Excreta
- Cow/Bullock 36 60-65%
- Buffalo 36 “
- Pig 78 “
- Chicken 62 “
13. Human Excreta 70 “
Production
Production
Hydrolysis
In general, hydrolysis is a chemical reaction in which the breakdown of water occurs to form H+ cations
and OH- anions. Hydrolysis is often used to break down larger polymers, often in the presence of an acidic
catalyst.
𝐶12 𝐻22 𝑂11 → 𝐶6 𝐻12 𝑂6
Acidogenesis
Acidogenesis is the next step of anaerobic digestion in which acidogenic microorganisms further break
down the Biomass products after hydrolysis. These fermentative bacteria produce an acidic environment in
the digestive tank while creating ammonia, H2, CO2, H2S, shorter volatile fatty acids, carbonic acids,
alcohols, as well as trace amounts of other byproducts.
𝐶6 𝐻12 𝑂6 → 2𝐶2 𝐻5 𝑂𝐻 + H2 + CO2
Production
Acetogenesis
In general, acetogenesis is the creation of acetate, a derivative of acetic acid, from
carbon and energy sources by acetogens. These microorganisms catabolize many of the products
created in acidogenesis into acetic acid, CO2 and H2.
2𝐶2 𝐻5 𝑂𝐻--> CH3COO- + H2 + CO2
Methanogenesis
Methanogenesis constitutes the final stage of anaerobic digestion in which methanogens create methane
from the final products of acetogenesis as well as from some of the intermediate products from hydrolysis
and acidogenesis.
CO2 + 4 H2 → CH4 + 2H2O
CH3COOH → CH4 + CO2
Processing of Biogas
The scrubbing of the biogas in order to remove impurities that are generated
during the digestion process such as CO2 (Carbon Dioxide) and H2S (Hydrogen
Sulfide)
Biogas go through a cleaning process, and becomes bio-methane.
A simple method for Hydrogen Sulfide utilizing steel wool in a glass bottle
For Carbon Dioxide removal, as well as additional Hydrogen Sulfide removal a
method of water spray crossflow can be used . In this method the Biogas enters
one end of a tube and experiences water streams flowing in the opposite
direction.
Benefits Of Biogas
Biogas can be explosive when mixed in the ratio of one part biogas
to 8-20 parts air.
Biogas leaks smell like rotten eggs (hydrogen sulfide)
Not feasible
Not Attractive on Large Scale
Little technological advancement
Contain Impurities
Cannot be easily stored .
Applications
Biogas in transport
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How to Feed Digesters
A good way to find out what is happening inside a digester is to attach a strip of
litmus paper and a thermometer to a long stick.
Put it down the digester inlet for five minutes, bring it out, read and record the
results, change the litmus paper and shake down the thermometer, and take a
second reading down the overflow pipe at the outlet end.
Compare the two sets of readings. Because of the different types of biological
activity going on at the beginning and end of digesters, there may also be a
difference in readings between the two ends.
Litmus paper is the easiest and cheapest way to measure pH levels, but it is not
the most accurate method. Litmus paper is useful for approximate, but not exact
readings.
A WELL-BUFFERED DIGESTER
It has been observed that a red or yellow biogas flame often means that the slurry is
slightly acid.
Adding a little lime or ash to the slurry mix should help adjust the acidity and restore
normal gas production.
Usually the answer is not to give the digester any medicine but rather to check and see
what it might be that you are doing wrong. If the bad practice can be stopped, the
digester will heal itself, usually.
The problem may be overfeeding of slurry or it may be a wrong balance of types of
plants and manure.
Using only sludge to feed the digester for a few days can help sometimes, but never
add any acid to a biogas digester that has become too base (alkaline).
Adding acid will only increase the production of hydrogen sulphide, which is of no use
at all.
48 BIOGAS DIGESTER
PRECAUTIONS AND PREVENTIVE MEASURES
Logically, fresh sludge from a digester looks very watery; there will
be very little solid matter floating on top of the bucket and very little
left in the bucket when we use the sludge to dilute fresh waste
going into the digester.
Everything is fine until a few months later when biogas production
drops off and nothing comes out of the outlet.
What our logical eyes did not see was solid matter suspended,
floating in the sludge, not much (only about ten percent), but
enough to make a difference in time--if the solids are not filtered
out of the sludge before it is used to make fresh slurry.
56 WORLDWIDE MARKET OF BIOGAS
COMPARISON OF WORLD’S BIOGAS UTILISATION
DESIGN OF BIO-DIGESTER
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How is biogas made ?
Biogas is made by fermenting organic waste in a biogas digester.
Small-Scale Digesters
Fixed Dome Biogas Plants
Floating Drum Plants
Balloon(Polythene Bag) Plants
Industrial Digester Types
Batch plants
Continuous-feed plants
Batch digesters
Horizontal digester
Vertical digester
Comparison between Vertical and horizontal
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In the vertical digester organic waste often escapes being "eaten" by the
bacteria. Slurry added one day can easily be withdrawn soon afterwards at the
nearby outlet, as incompletely digested waste.
In horizontal digesters the slurry must pass an area of maximum digestion on its
way from inlet to outlet, with no part of the slurry spending less time in the
digester than any other part.
From a practical point of view, above ground digesters are easier to get at to
repair and clean than underground digesters.
The problem of large scum layers is less for horizontal digesters because they
have a larger slurry surface area than vertical digesters of the same size.
Horizontal digesters do not usually have to be repaired or cleaned as often as
vertical digesters.
Given equal size and other factors, horizontal digesters will produce more
biogas than vertical digesters
FIXED DOME DIGESTER
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Fixed dome plant design: 1.Mixing tank I
with inlet pipe and sand trap. I
67 2.Digester. 3.Compensation and I
removal tank. 4.Gasholder. 5.Gaspipe. I Basic function of a fixed-dome
6.Entry hatch, with gastight seal. I biogas plant: 1.Mixing pit,
7.Accumulation of thick sludge. I 2.Digester, 3.Gasholder,
8.Outlet pipe. 9.Reference level. I 4.Displacement pit, 5.Gas pipe
10.Supernatant scum, broken up by I
varying level. I
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• A fixed-dome plant consists of a digester with a fixed, non-movable gas holder,
which sits on top of the digester.
• When gas production starts, the slurry is displaced into the compensation tank.
• Gas pressure increases with the volume of gas stored and the height difference
between the slurry level in the digester and the slurry level in the compensation
tank.
• The costs of a fixed-dome biogas plant are relatively low. It is simple as no moving
parts exist.
• There are also no rusting steel parts and hence a long life of the plant (20 years or
more) can be expected.
• The plant is constructed underground, protecting it from physical damage and
saving space.
• While the underground digester is protected from low temperatures at night and
during cold seasons, sunshine and warm seasons take longer to heat up the
digester.
• No day/night fluctuations of temperature in the digester positively influence the
bacteriological processes.
• The construction of fixed dome plants is labor-intensive, thus creating local
employment.
FUNCTIONS
• The gas is stored in the upper part of the digester. When gas production
commences, the slurry is displaced into the compensating tank.
• Gas pressure increases with the volume of gas stored, i.e. with the height
difference between the two slurry levels. If there is little gas in the gas-holder, the
gas pressure is low.
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ADVANTAGES DISADVANTAGES
• Low initial costs and long useful life- • Masonry gas-holders require
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span. special sealants and high technical
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• No moving or rusting parts involved. skills for gas-tight construction.
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• Basic design is compact, saves • Gas leaks occur quite frequently;
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space and is well insulated. fluctuating gas pressure complicates
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• Construction creates local gas utilization; amount of gas
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employment. produced is not immediately visible,
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• Advantages are the relatively low plant operation not readily
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construction costs, the absence of understandable.
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moving parts and rusting steel • Fixed dome plants need exact
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parts. If well constructed, fixed planning of levels.
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dome plants have a long life span. • Excavation can be difficult and
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The underground construction expensive in bedrock.
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saves space and protects the • Disadvantages are mainly the
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digester from temperature frequent problems with the gas-
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changes. The construction provides tightness of the brickwork gas holder
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opportunities for skilled local (a small crack in the upper brickwork
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employment. can cause heavy losses of biogas).
FLOATING DRUM DIGESTER
• In the case of the Low-Cost Polyethylene Tube Digester model which is applied
in Bolivia (Peru, Ecuador, Colombia, Centro America and Mexico), the tubular polyethylene
film (two coats of 300 microns) is bended at each end around a 6 inch PVC drainpipe and is
wound with rubber strap of recycled tire-tubes. With this system a hermetic isolated tank is
obtained.
75 BALLOON TYPE DIGESTER
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• A balloon plant consists of a heat-sealed plastic or rubber bag (balloon), combining
digester and gas-holder.
• The gas is stored in the upper part of the balloon. The inlet and outlet are attached directly
to the skin of the balloon.
• If the gas pressure exceeds a limit that the balloon can withstand, it may damage the skin.
• Therefore, safety valves are required. If higher gas pressures are needed, a gas pump is
required.
• Since the material has to be weather- and UV resistant, specially stabilized, reinforced
plastic or synthetic caoutchouc is given preference.
• Other materials which have been used successfully include RMP (red mud
plastic), Trevira and butyl. The useful life-span does usually not exceed 2-5 years.
ADVANTAGES DISADVANTAGES
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• Standardized prefabrication at I • Low gas pressure may require gas
low cost. I pumps.
• Low construction sophistication. I • Scum cannot be removed during
• Ease of transportation. I operation.
• Shallow installation suitable for I • The plastic balloon has a relatively
use in areas with a high I short useful life-span and is
groundwater table. I susceptible to mechanical
• High temperature digesters in I damage and usually not available
warm climates. I locally.
• Uncomplicated cleaning. I • In addition, local craftsmen are
• Emptying and maintenance. I rarely in a position to repair a
• Difficult substrates like water I damaged balloon. There is only
hyacinths can be used I little scope for the creation of local
I employment and, therefore,
I limited self-help potential.
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Other designs of bio-digesters
Should the digester be underground ?
Biogas digesters should be built above ground for several reasons. (At most, only a few
inches of the digester should be underground).
The closer the temperature of the slurry (the mixture of organic waste and water) inside the
digester is to 35 degrees centigrade/95 degrees Fahrenheit, the better it will be for the
biogas producing bacteria.
Underground digesters in hot climates will always be cooler than above ground digesters in
the same areas, which means underground digesters will, everything else being equal,
produce less gas.
In climates with cold weather, the extra expense of heating digesters will prove more
profitable in the long run than avoiding some of the cold by building underground.
The main advantage to building underground is that the dirt will help support the digester
walls.
The walls do not have to be as strong or expensive as the walls of above ground digesters.
But if an above ground digester is made well, the increased construction costs can be
rewarded with increased biogas production and a higher quality fertilizer.
Advantages and Disadvantages
to all possible construction materials
Concrete (using the ferro-cement method) may be the cheapest method, but
concrete digesters cannot be moved.
Concrete digesters have to be very carefully made if they are to be watertight and
airtight.
Concrete will stay warmer at night longer than metal or plastic, and that means
more gas.
Metal can rust; the welding and painting must be done perfectly.
The zinc in galvanized iron can kill biogas producing bacteria, so the inside walls of
metal digesters must be painted.
Once made, plastic and metal digesters are less likely to leak. When empty, plastic
bag digesters can be moved. e Plastic and concrete will not rust.
Important points to consider during design
If a digester is too long and thin, the fresh slurry will not mix properly with the active
bacteria and the digestion process will be slow in starting.
Fresh slurry should come into contact with the slurry of previous days, which in turn,
should be in the active stages of decomposition leading to the final stage of methane
production.
If a digester is too short or too wide, the physical and biological steps will not be spread
out enough.
Square and round digesters produce less gas and a less sanitary fertilizer than long
digesters.
Today's fresh slurry is mixed at random with previous slurry, some will be taken out before
it has been completely digested, and some will stay in the digester long after it has
been completely digested.
The proportions of diameter to length of a digester is not very critical. A ratio of five in
length to one in diameter is best. Ratios between 8/1 and 3/1 length to diameter are
the outside extremes of digester proportions.
How to make more profits?
1. Include fish ponds and/or other uses for the fertilizer that is produced.
2. Be large enough to benefit from the addition of a stationary engine to
the system, fueled by the biogas, and heating the digester with the
excess engine heat.
3. Consider making financial payments to the investors in the business or
cooperative as an alternative to using the fuel and fertilizer as payments.
4. Consider trading for or buying organic wastes for the digester
5. Consider building central piggeries, chicken coops, and cattle stalls
animals owned by cooperative members or business partners in order to
collect as much organic waste as possible.
Comparison of Different designs
Plastic
Factors Fixed dome Floating drum Tubular design
containers
Gas storage Internal Gas Internal Gas Internal Internal Gas
storage up to storage drum eventually storage drum
20 m³ (large) size (small) external sizes (small)
plastic bags
Gas pressure Between 60 Upto 20 mbar Low, around 2 Low around
and 120 mbar mbar 2mbar
Skills of High; masonry, High; masonry, Medium; Low; plumbing
contractor plumbing plumbing, plumbing
welding
Durability Very high >20 High; drum is Medium; Medium
years weakness Depending on
chosen liner
Agitation Self agitated Manual Not possible; Evtl Manual
by Biogas steering plug flow type steering
pressure
Methane High Medium Low Medium
emission
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Thank you