Based on paper EFFECTS OF TEMPARATURE AND PH ON

ANTIMICROBIAL ACTIVITY OF SILVER ANANOPARTICLES

Antibacterial activity was enhanced rapidly with increasing AgNPs concentrations.
AgNPs displayed significant antibacterial activity with a concentration at 5 mg/L
or more. At 5 mg/L concentration of AgNPs, nearly 50% of the bacteria in the
o
suspension at 37 C were killed. In comparison, less than 20% killing was achieved
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at 4 C. When the concentration of AgNPs reached the highest test concentration of 7
o
mg/L, 60% of the bacteria were killed at 37 C. This antibacterial activity was
o
almost three times higher than that at 4 C, under which condition only 20% of the
bacteria exposed to AgNPs at the concentration of 7 mg/L were killed. This
observation is in line with the general disinfection behavior of chemical
disinfectants wherein microbial inactivation decreases at lower temperature[29]
whereas Whereas on the basis of this experiment when AgNPS are chemically
synthesized it shows that nanosolution is more effective in higher temperature
while AgNPS are green synthesized nanosolution is more effective on lower
temperature

EFFECTS OF PH
The control AgNPs started to show evident antibacterial effect at the concentration
of 5 mg/L, with the mortality of nearly 50%, and almost all of the bacteria were killed
when the AgNPs concentration reached 10 mg/L. The AgNPs treated with alkaline
pH exhibited approximately the same antibacterial effect as the control AgNPs. On
the contrary, AgNPs treated with acidic pH did not exhibit any antibacterial effect
until the AgNPs concentration was increased to 7.5 mg/L. Only half of the bacteria
were killed when the concentration of AgNPs reached 10 mg/L, while a mortality
close to 100% were achieved for the control AgNPs at the same concentration.
These results clearly demonstrate that treatment of AgNPs at pH 2.0 caused a
much feeble antibacterial activity compared to control AgNPs without pH
treatment, while antibacterial activity of AgNPs treated with pH 12.0 was similar
with the treatment-free AgNPs. Whereas in this experiment it shows that for
alkaline effect MIC formed at 80 cm for E.coli for 2mM TA, TSC the zone of
inhibition is 1.2 cm whereas for Staphylococcus The zone of inhibition at 80 mcg is
1.6cm for the same combination .
For green synthesis it shows that the MIC for acidic effect was found at 10 mcg
for both the combinations for Staphylococcus and E.coli as well. The zone of
inhibition for E.coli is 1.1 cm for combination Tulsi and for staphylococcus is
1.8cm.