S kandan

8sgnmeot IMG198TO46
Sem Sec R
raocgenfc Anmals

Introdu.cion
a tron sqenic onfmal s one that carrie a gene that hos beon deltlerutely

nsetted tote tts geneme

he erocess mixing do oenes 1 called transgenests

be transmited to
hergn genes are fo setedl Pdo germ Ane o ontmol, Ae tt an

pragen
other anfnals ath
tronsaenic technolegy hox led to derelopment o he,te toca 4
altered genetfic proiles ahich ore tkcu to monefock

the tronsans qeofc aafnal oa6uermoukt created b Ralh BRoser 4 Rtchar)

fn 1982

.tcm created by ogeHfog a numan groath hormone gene m mouse

Qenome

tho oing c much arqer than the arents

ffoduxtion_eTroosgenfc Rnimals

A) Hethadolog-

STep : Constructian ronsgene

* trans gene mde uo d 3 partg bromoter, gene to be exbtossed & termoatic

6eqenie
 romoter Po A
Geqenre Gnene Seqende

Step 2: Intreductien o preRgn gene frte anfna

Xpronucleor mfcreTnferfon method

Xermaryonkc Stem cel methed

Bfcroofectfon ethod-

aemale anïmal Ts Super eNulated 4 eggs are collectes

keggs are eize tn vitre

K Habsaene containiag seluton 1S fafecte te male pooucleus usiag-

mfcropete
X e99s oth ransqenes are kept overn+gt o an tacubator to dereloe a

2 cell tage

Keggs are hen Tmplanted rote uterus o pseuda- pregnat emale

hold Poc_pi pete polar body
nuteolus
nertton needle
emale Contalniag DNA 6alutian
orbouclewS mgle
od noeleus

c Embycoie em (ell Hethod

transgenik animala a n be created by manfpulatfog. embyomic tem _cella.

x_ScelUs are ebtafoed rem fnner cell mass blottoqt

 * ronSLene ncorporatel îorto ES
cela mfcro fnfectifbo, retpvrus or by

electhocorhation
xtransgenic dem cell ave grow0 th Vitre

Thy are then 1tel trde a

Voetoat tmplanteolTmo
a hott uterus To

re normay

D Blacto cut Rcroinfectfon

Step 3 Screenn transqenfc posthres

*tronsgenie proqenies ore Screeneok bu PCRto xamine.stteo nadporatiam

h e 9ene
K Same transgene) may_pot be etpresse fote gmtes Rota a

fYanakpahionaly Yaactre Ste

Step 4: Futheran?mal breeling fs done ta auttia moaimal expreslan

beterezugevs ags ore mote k orm Docnoos

Shreng

Aralblems
HuHple in setfon prcefns

lega f66oeA regaarding e nsertiam t o an essenttal gene

nsetio ote a eng leadtng te gene Atlenctog

loseion scte a dlleren drea can_alect ene regukation

ooalonegeni. Ant.mals

Xfocreased greuwh4 6ze

*grouth hormone gene Tosete 1ote
OnSRencsalmon ous about lo-U Hmesner than amal
E)_ Iransqerkc (atie:

madeto pmdue ototen3Aacte eta A.inte øms io thetr milea

R_pfan ea cos estat to mad coku

dRseoke
Traagent flaakey
AANDE a the raazgenîc mamrea bom fa 200c
K Apet ack abardS, ANDI dtnnds ase

NDporea that traosaenicpmatea an be (heates_a can ezprrss a

gene delMerea) hta hor aeme

Imaoctaoce
mecal importane.

drseae model

bioreactors arma casticals

agncoural tmortance

seoe eastort anfmads

 mWk,meat, e9s d co0ol prodvction

fodUstRa Tmortane-

* toxicrt Sensistire traugenfc anfna to teyt chemfcals

ssusea
bluriag Aiaes between spede reatfnq transgeni conbiretiora

heath Rshs
iotolel
e T m eecta on environment hen trougefc anfmala are heleaze

that TtCrDng to oeate antmals thet cole solle dot

many bie ethict_aqire

to eetic alteraiion .

onclusian
tramgenfc techoelopu a tell thada under ontoot evelution

tholds Graat potential Ia man ields like agicaktue, mesicine, indUstne ,et
man ronggenic anmals haane been Succe\olu create